Method for inducing differentiation and seedling formation of passion fruit stem section callus

Abstract

The invention provides a method for inducing differentiation and seedling formation of passion fruit stem section callus. The method comprises the following steps of: establishing a sterile system bytaking a mature plant passion fruit stem as an explant, and performing induction culture and subculture. According to the method system, the problems that the passion fruit is developed in rhizome, rooted on the node, thick in root hair of the rhizome, rich in endophyte in woody stem, sensitive to bactericide reaction, easy to discolor, difficult in establishment of the sterile system and the likeare solved, and the bottleneck that the passion fruit callus is difficultly differentiated into buds and the rapid propagation technology is hindered is solved.

Description

technical field

[0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for inducing callus on cut surface of passion fruit stem to differentiate into seedlings. Background technique

[0002] Passion fruit: Passion fruit, also known as passion fruit, Brazil nut, egg fruit. Passion fruit is rich in amino acids, multivitamins, carotenoids, superoxide dismutase (SOD), selenium and various trace elements needed by the human body. Passion fruit is known as the king of VC in fruits. Regular consumption of passion fruit has the functions of refreshing the mind, beautifying the skin, promoting body fluid and quenching thirst, helping digestion, reducing phlegm and relieving cough, relieving constipation, promoting blood circulation and strengthening the body, and improving human immunity. At present, the quality of passion fruit seedlings is uneven, the seedlings are infected with diseases (mainly viral diseases), and the varieties are...

Examples

Embodiment 1

[0021] (1) Collection and disinfection of explants: use mature passion fruit stems as explants, select new shoots of vigorously growing and disease-free plants, wash the surface of tap water, soak in 1wt.% sodium hypochlorite solution for 15 min, and rinse with tap water Rinse well, sterilize in 75% alcohol by volume for 10 seconds, then sterilize with 0.1wt.% mercuric chloride for 5 minutes, and rinse with sterile water for 4 times;

[0022] (2) Induction culture: Under sterile conditions, the sterilized branches were cut into 1.2 cm stem segments and inserted upright into the induction medium. The light was continuously illuminated for 8 h every day, and the light intensity was 1200 lx. The culture temperature was 27 °C during the day and 21°C, cultivate for 130 days;

[0023] (3) Subculture; the seedlings formed on the induction medium were cut into stems with buds under aseptic conditions and transplanted into the growth medium, with continuous light for 12 h every day, li...

Embodiment 2

[0029] (1) Collection and disinfection of explants: use mature passion fruit stems as explants, select new shoots of vigorously growing and disease-free plants, wash the surface of tap water, soak in 1wt.% sodium hypochlorite solution for 15 min, and rinse with tap water Rinse well, sterilize in 75% alcohol by volume for 10 seconds, then sterilize with 0.1wt.% mercuric chloride for 6 minutes, and rinse with sterile water for 5 times;

[0030] (2) Induction culture: Cut the sterilized shoots into 1.3 cm stems and insert them upright into the induction medium under sterile conditions, continuously light for 8 hours a day, light intensity 1000 lx, culture temperature 21-27 ℃, cultivate 130 days;

[0031] (3) Subculture; the seedlings formed on the induction medium were cut into stems with buds under aseptic conditions and transplanted into the growth medium, with continuous light for 12 h every day, light intensity of 2 000 lx, and culture temperature of 25± 1 ℃, cultivated for ...

Embodiment 3

[0038] (1) Collection and disinfection of explants: use mature passion fruit stems as explants, select new shoots of vigorously growing and disease-free plants, wash the surface of tap water, soak in 1wt.% sodium hypochlorite solution for 15 min, and rinse with tap water Rinse well, sterilize in 75% alcohol by volume for 10 seconds, then sterilize with 0.1wt.% mercuric chloride for 6 minutes, and rinse 4 times with sterile water;

[0039] (2) Induction culture: Under sterile conditions, cut the sterilized branches into 1.3 cm stems and insert them upright into the induction medium, with continuous light for 8 hours a day, light intensity of 1500 lx, and culture temperature of 21-27 °C. 130 days;

[0040](3) Subculture; the seedlings formed on the induction medium were cut into stems with buds under aseptic conditions and transplanted into the growth medium, with continuous light for 12 h every day, light intensity of 2500 lx, and culture temperature of 25±1 ℃, cultivated for ...

technical field

[0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for inducing callus on cut surface of passion fruit stem to differentiate into seedlings. Background technique

[0002] Passion fruit: Passion fruit, also known as passion fruit, Brazil nut, egg fruit. Passion fruit is rich in amino acids, multivitamins, carotenoids, superoxide dismutase (SOD), selenium and various trace elements needed by the human body. Passion fruit is known as the king of VC in fruits. Regular consumption of passion fruit has the functions of refreshing the mind, beautifying the skin, promoting body fluid and quenching thirst, helping digestion, reducing phlegm and relieving cough, relieving constipation, promoting blood circulation and strengthening the body, and improving human immunity. At present, the quality of passion fruit seedlings is uneven, the seedlings are infected with diseases (mainly viral diseases), and the varieties are...

Examples

Embodiment 1

[0021] (1) Collection and disinfection of explants: use mature passion fruit stems as explants, select new shoots of vigorously growing and disease-free plants, wash the surface of tap water, soak in 1wt.% sodium hypochlorite solution for 15 min, and rinse with tap water Rinse well, sterilize in 75% alcohol by volume for 10 seconds, then sterilize with 0.1wt.% mercuric chloride for 5 minutes, and rinse with sterile water for 4 times;

[0022] (2) Induction culture: Under sterile conditions, the sterilized branches were cut into 1.2 cm stem segments and inserted upright into the induction medium. The light was continuously illuminated for 8 h every day, and the light intensity was 1200 lx. The culture temperature was 27 °C during the day and 21°C, cultivate for 130 days;

[0023] (3) Subculture; the seedlings formed on the induction medium were cut into stems with buds under aseptic conditions and transplanted into the growth medium, with continuous light for 12 h every day, li...

Embodiment 2

[0029] (1) Collection and disinfection of explants: use mature passion fruit stems as explants, select new shoots of vigorously growing and disease-free plants, wash the surface of tap water, soak in 1wt.% sodium hypochlorite solution for 15 min, and rinse with tap water Rinse well, sterilize in 75% alcohol by volume for 10 seconds, then sterilize with 0.1wt.% mercuric chloride for 6 minutes, and rinse with sterile water for 5 times;

[0030] (2) Induction culture: Cut the sterilized shoots into 1.3 cm stems and insert them upright into the induction medium under sterile conditions, continuously light for 8 hours a day, light intensity 1000 lx, culture temperature 21-27 ℃, cultivate 130 days;

[0031] (3) Subculture; the seedlings formed on the induction medium were cut into stems with buds under aseptic conditions and transplanted into the growth medium, with continuous light for 12 h every day, light intensity of 2 000 lx, and culture temperature of 25± 1 ℃, cultivated for ...

Embodiment 3

[0038] (1) Collection and disinfection of explants: use mature passion fruit stems as explants, select new shoots of vigorously growing and disease-free plants, wash the surface of tap water, soak in 1wt.% sodium hypochlorite solution for 15 min, and rinse with tap water Rinse well, sterilize in 75% alcohol by volume for 10 seconds, then sterilize with 0.1wt.% mercuric chloride for 6 minutes, and rinse 4 times with sterile water;

[0039] (2) Induction culture: Under sterile conditions, cut the sterilized branches into 1.3 cm stems and insert them upright into the induction medium, with continuous light for 8 hours a day, light intensity of 1500 lx, and culture temperature of 21-27 °C. 130 days;

[0040](3) Subculture; the seedlings formed on the induction medium were cut into stems with buds under aseptic conditions and transplanted into the growth medium, with continuous light for 12 h every day, light intensity of 2500 lx, and culture temperature of 25±1 ℃, cultivated for ...

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