A kind of alkaloid compound, preparation method and application

A technology of alkaloids and compounds, which is applied in the preparation of sugar derivatives, chemical instruments and methods, drug combinations, etc., can solve the problems of poor efficacy of complications, prolonged medication time, and prone to drug resistance, etc., to achieve beneficial The effects of large-scale production promotion, low cost, and simple extraction and separation methods

CN111518149BActive Publication Date: 2021-10-15XI AN JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2021-10-15

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Abstract

The present invention belongs to natural extraction compounds and their preparation methods and applications. Although there are many types of existing hypoglycemic drugs, they still have large side effects, poor curative effect on complications, and drug resistance is prone to appear with prolonged medication time. Technical problem, provide a kind of alkaloid compound, preparation method and application, the molecular formula of alkaloid compound is C 46 h 63 N 3 o 23 , is an indole glycoside dimer alkaloid, and the preparation method includes extracting the total extract from Jiuzilian, extracting the total extract, and performing column chromatography separation on the n-butanol extraction layer. The preparation method is simple and effective to obtain an alkaloid compound with high purity and low cost. It has been verified that the alkaloid compound can be applied to anti-inflammatory drugs and anti-diabetic drugs.
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Description

technical field

[0001] The invention belongs to natural extraction compounds and their preparation methods and applications, in particular to an alkaloid compound, preparation methods and applications. Background technique

[0002] In recent years, diabetes has become a common disease worldwide. There are many patients, and there are also a large number of pre-diabetic patients. Among this huge number of diabetic reserve army, one-third of them will eventually develop into real diabetes. The situation is dire. Although a variety of hypoglycemic drugs other than insulin are currently clinically used, on the one hand, these drugs have different degrees of side effects and are not effective in treating diabetic complications; on the other hand, with the prolongation of medication time, Prone to secondary failure, ie drug resistance. There are 67% of diabetic patients whose blood sugar control is not up to standard, which has caused a heavy life and economic burden to the fami...

Examples

preparation example Construction

[0051] The invention provides a kind of preparation method of alkaloid compound, specifically as follows:

[0052] S1, take a certain quality of Jiuzilian dry rhizome;

[0053]Use methanol or ethanol whose volume is 1 to 8 times the mass of the dried rhizome of Jiuzilian, heat and reflux for extraction 1 to 6 times near their respective boiling points, each time for 1 to 3 hours; wherein, if ethanol is used, the volume fraction of ethanol is 10 ~95%.

[0054] Alternatively, extract by cold soaking at room temperature for 1 to 5 times, each time for 1 to 3 hours;

[0055] Merge each extract and concentrate to obtain the total extract;

[0056] S2, the total extract obtained through step S1 is extracted at least once through ethyl acetate and n-butanol successively;

[0057] Liquid-liquid extraction is possible, that is, the total extract is suspended in water, the volume ratio of the total extract to water is 1:1 to 1:4, and the extract liquid is obtained, which is sequentia...

Embodiment 1

[0069] A kind of preparation method of alkaloid compound, concrete method is as follows:

[0070] 1) Take 20 kg of dried rhizomes of Jiuzilian, heat and reflux extract with methanol whose volume is 6 times the mass of dried rhizomes of Jiuzilian, extract 4 times, each time for 2 hours, combine the extracts to recover the solvent under reduced pressure, and obtain the total extract;

[0071] 2) Suspend the total extract in water of 4 times the amount, extract 4 times with ethyl acetate, then extract 4 times with n-butanol equal volume, and remove the organic solvent under reduced pressure in the extraction layer to obtain ethyl acetate and n-butyl alcohol respectively. Alcohol layer.

[0072] 3) Take 200 g of the n-butanol extraction layer, firstly, by using silica gel column chromatography, chloroform / methanol is 100:0~0:100 in volume ratio (v / v) for gradient elution, collect a fraction every 800mL, and pass through TLC A total of 15 fractions were obtained after identificati...

Embodiment 2

[0076] A kind of preparation method of alkaloid compound, concrete method is as follows:

[0077] 1) Take 20 kg of dried rhizomes of Jiuzilian, heat and reflux extraction with ethanol whose volume is 8 times the mass of dried rhizomes of Jiuzilian, and extract them under reflux for 3 times, each time for 3 hours, wherein the volume fraction of ethanol is 10%, and the combined extracts are decompressed Recover the solvent to obtain the total extract;

[0078] 2) Suspend the total extract in 3 times the amount of water, first extract 3 times with ethyl acetate, then extract 3 times with an equal volume of n-butanol, remove the organic solvent from the extraction layer to obtain ethyl acetate and n-butyl alcohol respectively Alcohol layer.

[0079] 3) Take 80 g of the n-butanol extraction layer, firstly, by using silica gel column chromatography, chloroform / methanol is 100:0~0:100 in volume ratio (v / v) for gradient elution, collect a fraction every 500mL, and pass through TLC A...