Core-shell metal matrix, preparation and application thereof

A metal matrix, core-shell technology, applied in the field of chemical synthesis and characterization of micro-nano materials, can solve problems such as small molecule detection obstacles, impact on map quality, fragility and fragmentation, etc., to solve background interference and hot spot effects, detection High throughput, rapid detection of the effect

CN111747447AActive Publication Date: 2020-10-09浙江亿纳谱生命科技有限公司
9 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2020-10-09

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a core-shell metal matrix, preparation and application thereof, and relates to chemical synthesis and characterization of micro-nano materials and application of a belonging matrix-assisted laser desorption / ionization mass spectrometry technology to the fields of molecular biology and medical test. According to the present invention, a customized and synthesized micro-nanoparticle material is adopted as the matrix, the defects of the traditional organic matrix are effectively overcome, the matrix is suitable for a variety of different biological samples, the rapid, high-throughput and high-sensitivity detection of the metabolite small molecules in the biological samples is effectively achieved, and the matrix can be applied in a variety of clinical detection applications.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the chemical synthesis and characterization of micro-nano materials, in particular to the application of its matrix-assisted laser desorption / ionization mass spectrometry technology in the fields of molecular biology and medical detection. Background technique

[0002] Matrix-assisted laser desorption / ionization mass spectrometry (MALDI-MS), that is, the analyte and the matrix material are mixed to form a co-crystal, and crystallized by laser irradiation, the matrix efficiently transfers the laser energy to the analyte molecules so that the molecules are ionized to facilitate analyzed by subsequent mass spectrometry. Traditional MALDI-MS is widely used in the detection of biological macromolecules such as nucleic acid and protein. After years of development, MALDI-MS has also been used to detect small organic molecules, especially small biological molecules (such as small molecule metabolites).

[0003] MALDI-TOF-MS has the a...

Examples

Embodiment 1

[0057] Example 1: Detection of Small Molecules in Plasma Samples

[0058] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometry, positive ion reflection mode; the prepared core-shell structure metal nanoparticles are used as the matrix to dilute the plasma sample in a certain proportion;

[0059] (2) Prepare the plasma sample on the mass spectrometer target plate and dry it at room temperature;

[0060] (3) detect under the mass spectrometer, and analyze the mass spectrum image, such as figure 1 shown.

Embodiment 2

[0061] Example 2: Detection of Small Molecules in Serum Samples

[0062] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometry, positive ion reflection mode; the prepared core-shell structure metal nanoparticles are used as matrix to dilute serum samples in a certain proportion;

[0063] (2) Serum sample preparation is carried out on the mass spectrometry target plate, and dried at room temperature;

[0064] (3) detect under the mass spectrometer, and analyze the mass spectrum image, such as Figure 4 shown.

Embodiment 3

[0065] Example 3: Detection of Small Molecules in Urine Samples

[0066] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization time-of-flight mass spectrometry, cationic reflection mode; the prepared core-shell structure metal nanoparticles are used as the matrix to dilute the urine sample in a certain proportion;

[0067] (2) Prepare the urine sample on the mass spectrometer target plate and dry it at room temperature;

[0068] (3) detect under the mass spectrometer, and analyze the mass spectrum image, such as Figure 5 shown.