Camellia oleifera high-yield and low-yield variety group metabolic expression profile marker identification method

An identification method and expression profile technology, which are applied in the field of identification of metabolic expression profile markers for high-yield and low-yield varieties of Camellia oleifera, and achieve the effects of scientific method, promotion of Camellia oleifera industrialization development, and simple sampling method.

CN112034070AActive Publication Date: 2020-12-04福建省沙县水南国有林场
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2020-12-04

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Abstract

The invention provides a camellia oleifera high-yield and low-yield variety group metabolic expression profile marker identification method, which belongs to the technical field of camellia oleifera breeding. At least three high-yield and low-yield camellia oleifera varieties are selected as a high-yield group and a low-yield group respectively; tender shoots are collected from the upper portion,the middle portion and the lower portion of each camellia oleifera single plant in the high-yield group or the low-yield group, and the collected tender shoots are mixed by taking the groups as units;a tender shoot sample of a high-yield group or a low-yield group is extracted by using an alcohol solvent, the solvent is removed, the obtained dark brown paste of the high-yield group or the low-yield group is diluted, analysis is carried out by using a GS-MS, and differential compounds in the metabolic expression profile of the high-yield group and the metabolic expression profile of the low-yield group are compared; and a compound with the content reaching a significant difference level as a marker to breed a high-yield camellia oleifera variety. The method provided by the invention is scientific, the sampling method is simple, the analysis method is convenient and rapid, and the method is suitable for camellia oleifera improved variety breeding and genetic improvement and has a greatpromotion effect on camellia oleifera industrialization development.
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Description

technical field

[0001] The invention belongs to the technical field of camellia oleifera breeding, in particular to a method for identifying markers of metabolic expression profiles of high-yield and low-yield varieties of camellia oleifera. Background technique

[0002] Camellia oleifera is an important woody oil tree species in my country and one of the four largest woody oil tree species in the world, mainly distributed in southern provinces. Its product camellia oil is a high-grade edible oil, and its fatty acid composition is similar to that of olive oil. Long-term consumption is very beneficial to human health. Camellia oleifera is planted on the mountain and does not compete with food for land, providing a large number of employment opportunities for forest farmers in mountainous areas. The camellia oleifera industry is a characteristic and advantageous industry in my country. The development of the camellia oleifera industry can not only ensure national grain and oi...

Examples

Embodiment 1

[0048] Three high-yielding camellia oleifera trees (numbered A, B, and C) were selected in Shuinan State-owned Forest Farm, Shaxian County, Fujian Province. The average fruit yield per plant in 2017-2019 was 2.62, 2.35, and 1.5Kg in turn. To build a high-yield group, select 3 plants For low-yield Camellia oleifera varieties (numbered D, E, and F), the average fruit yield per plant from 2017 to 2019 was 0.49, 0.46, and 0.01Kg, respectively, and the low-yield group was constructed.

[0049] On May 12, 2020, the sample BULK method was adopted, and 5 shoots were collected from the upper, middle, and lower parts of each individual plant, and mixed by each plant first, and then mixed by each group.

[0050] Take 100g of fresh mixed samples from the high-yield group and the low-yield group respectively, soak them in 1000mL of anhydrous methanol for 3 days, pour out the solvent and collect it, repeat 3 times, combine all the solutions obtained from the 3 times, then evaporate the solve...