A portable panoramic cell analysis system and panoramic microscopic imaging method thereof

Abstract

The embodiment of the present invention discloses a portable panoramic cell analysis system and a panoramic microscopic imaging method thereof. The portable panoramic cell analysis system includes a translation bracket, a panoramic microscopic imaging module, a stage and a light source; the translation bracket can translate in a first plane, and the panoramic microscopic imaging module is connected to the translation bracket; the panoramic microscopic imaging module includes a panoramic image acquisition unit and a microscopic magnification unit, and the effective working distance L1 between the panoramic image acquisition unit and the stage satisfies 30mm≤L1≤100mm; the focal length f of the objective lens satisfies 2mm≤f≤10mm. The panoramic microscopic imaging module is driven to move in the first plane by the translation bracket to realize real-time panoramic microscopic imaging; by reasonably setting the effective working distance between the panoramic image acquisition unit and the stage and the focal length of the objective lens, the panoramic microscopic imaging module is small and compact while ensuring clear panoramic imaging, thereby increasing the integration and portability of the portable panoramic cell analysis system.

Description

Technical Field

[0001] The embodiments of the present invention relate to the field of microscopic imaging technology, and in particular to a portable panoramic cell analysis system and a panoramic microscopic imaging method thereof. Background Art

[0002] Microscopic imaging technology is currently being used more and more, and is widely used in teaching, scientific research, cell analysis and other fields, helping people to achieve cognition of the microscopic field and playing an increasingly important role.

[0003] The observation of microscopic objects by a microscope depends on its own magnification. The larger the magnification, the smaller the field of view. When it is necessary to observe the overall situation of a large field of view, it is necessary to use stitching imaging technology to stitch different fields of view together to form an overall image within a large field of view. At present, the main method of stitching technology in the field of microscopic imaging is to take multiple adjacent pictures and then stitch them together, which cannot achieve real-time panoramic imaging. Summary of the invention

[0004] In view of this, an embodiment of the present invention provides a portable panoramic cell analysis system and a panoramic microscopic imaging method thereof to solve the technical problem that real-time panoramic microscopic imaging cannot be achieved in the prior art.

[0005] In a first aspect, an embodiment of the present invention provides a portable panoramic cell analysis system, characterized in that it includes a translation support, a panoramic microscopic imaging module, a stage and a light source;

[0006] The translation bracket can translate in a first plane, and the panoramic microscopic imaging module is connected to the translation bracket; wherein the first plane is parallel to the plane where the stage is located;

[0007] The panoramic microscopic imaging module includes a panoramic image acquisition unit and a microscopic magnification unit; along the direction perpendicular to the stage, the effective working distance L1 between the panoramic image acquisition unit and the stage satisfies 30mm≤L1≤100mm; the microscopic magnification unit at least includes a magnifying objective lens group, the magnifying objective lens group includes at least one objective lens, and the focal length f of the objective lens satisfies 2mm≤f≤10mm.

[0008] Optionally, the panoramic image acquisition unit includes a photoelectric conversion subunit, and the photoelectric conversion subunit is electrically connected to an external image processing device.

[0009] Optionally, the panoramic image acquisition unit includes a panoramic imaging camera.

[0010] Optionally, the panoramic microscopic imaging module is detachable.

[0011] Optionally, the portable panoramic cell analysis system further comprises a lifting component, the translation bracket is disposed on the lifting component, and the panoramic microscopic imaging module is detachably disposed on the translation bracket.

[0012] Optionally, when the portable panoramic cell analysis system is in working state, the panoramic microscopic imaging module is arranged on a side of the stage away from the light source;

[0013] An opening is formed on the stage; the panoramic microscopic imaging module includes a first part close to one side of the stage and a second part away from the side of the stage, and the vertical projection of the first part on the plane where the stage is located is located in the opening; when the portable panoramic cell analysis system is in a non-working state, the first part is nested in the opening.

[0014] Optionally, the distance between the panoramic image acquisition unit and the microscopic magnification unit is adjustable along a direction perpendicular to the stage.

[0015] Optionally, the light source is detachable.

[0016] Optionally, the portable panoramic cell analysis system further includes a lifting component, and the light source is detachably mounted on the lifting component.

[0017] Optionally, when the portable panoramic cell analysis system is in working state, the light source is arranged on a side of the stage away from the panoramic microscopic imaging module;

[0018] An opening is formed on the stage; a vertical projection of the light source on the plane where the stage is located is located in the opening; when the portable panoramic cell analysis system is in a non-working state, the light source is nested in the opening.

[0019] Optionally, the magnifying objective lens group includes a plurality of objective lenses with different magnifications.

[0020] Optionally, the microscope magnification unit further includes a magnifying eyepiece group, and the magnifying eyepiece group is located on a side of the magnifying objective lens group away from the stage;

[0021] The magnifying eyepiece set includes a plurality of eyepieces with different magnifications.

[0022] Optionally, the magnifying objective lens group includes a plurality of objective lenses with different magnifications;

[0023] The microscopic magnification unit further includes a magnifying eyepiece set, wherein the magnifying eyepiece set includes a plurality of eyepieces with different magnifications;

[0024] One of the eyepieces and one of the objective lenses form a combined lens.

[0025] Optionally, the size of the panoramic image acquisition unit is less than or equal to 1 / 3 inch.

[0026] In a second aspect, an embodiment of the present invention further provides a panoramic microscopic imaging method of a portable panoramic cell analysis system, using the portable panoramic cell analysis system described in the first aspect, comprising:

[0027] Determine the panoramic microscopy imaging path;

[0028] The translation support is controlled according to the panoramic microscopic imaging path to drive the panoramic microscopic imaging module to move, so as to obtain a panoramic microscopic image of the observed sample.

[0029] Optionally, controlling the translation support to drive the panoramic microscopic imaging module to move according to the panoramic microscopic imaging path to obtain a panoramic microscopic image of the observed sample includes:

[0030] Continuously adjusting the distance between the panoramic image acquisition unit and the microscopic magnification unit to a preset distance along a direction perpendicular to the stage;

[0031] At the preset distance, using the portable panoramic cell analysis system to perform microscopic imaging on a single screen of the observed sample;

[0032] The translation support is controlled according to the panoramic microscopic imaging path to drive the panoramic microscopic imaging module to move, so as to obtain a panoramic microscopic image of the observed sample.

[0033] The portable panoramic cell analysis system and the panoramic microscopic imaging method thereof provided by the embodiments of the present invention, in the portable panoramic cell analysis system, a translation bracket is provided to be connected with a panoramic microscopic imaging module, the panoramic microscopic imaging module is driven to move in a first plane by the translation bracket, and real-time panoramic microscopic imaging is achieved based on the panoramic imaging function of the panoramic microscopic imaging module, thereby solving the technical problem that real-time panoramic microscopic imaging cannot be achieved in the prior art; and, the embodiments of the present invention reasonably set the effective working distance between the panoramic image acquisition unit and the stage in the panoramic microscopic imaging module and the focal length of the objective lens, so that the panoramic microscopic imaging module is small and compact while ensuring clear panoramic imaging, thereby increasing the integration and portability of the portable panoramic cell analysis system. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0035] Figure 1 It is a structural schematic diagram of a panoramic microscopic imaging device in the prior art;

[0036] Figure 2is a schematic structural diagram of a portable panoramic cell analysis system provided by an embodiment of the present invention;

[0037] Figure 3 is a schematic diagram of a top view of a magnifying objective lens assembly provided by an embodiment of the present invention;

[0038] Figure 4 is a schematic diagram of a microscopic image in a microscopic imaging field of view provided by an embodiment of the present invention;

[0039] Figure 5 is a structural schematic diagram of a panoramic image acquisition unit provided by an embodiment of the present invention;

[0040] Figure 6 is a schematic structural diagram of another portable panoramic cell analysis system provided by an embodiment of the present invention;

[0041] Figure 7 is a structural schematic diagram of a panoramic microscopic imaging module provided by an embodiment of the present invention;

[0042] Figure 8 is a schematic diagram of a top view of a stage provided by an embodiment of the present invention;

[0043] Fig. 9 is a schematic structural diagram of another portable panoramic cell analysis system provided by an embodiment of the present invention;

[0044] Fig.10 is a schematic structural diagram of another portable panoramic cell analysis system provided by an embodiment of the present invention;

[0045] Fig.11 is a schematic diagram of a top view structure of a magnifying eyepiece assembly provided by an embodiment of the present invention;

[0046] Fig.12 is a structural schematic diagram of a combined lens provided by an embodiment of the present invention;

[0047] Fig.13 is a structural schematic diagram of another panoramic image acquisition unit provided by an embodiment of the present invention;

[0048] Fig.14 It is a schematic flow chart of a panoramic microscopic imaging method provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0049] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be fully described below in combination with the drawings in the embodiments of the present invention through specific implementation methods. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work fall within the protection scope of the present invention.

[0050] Figure 1 is a schematic diagram of the structure of a panoramic microscopic imaging device in the prior art, such as Figure 1 As shown, when it is necessary to observe the overall situation of a large field of view, and one imaging cannot observe all target objects, multiple microscopic imaging is required. The prior art is to drive the sample plate 1 to translate through the translation stage 2, and the microscopic imaging device 3 obtains an imaging picture based on each movement, and realizes the splicing of different fields of view through the splicing imaging technology to form an overall image within a large field of view. However, the above panoramic microscopic imaging method cannot achieve real-time panoramic imaging.

[0051] Based on the above technical problems, an embodiment of the present invention provides a portable panoramic cell analysis system, including a translation bracket, a panoramic microscopic imaging module, a stage and a light source; the translation bracket can translate in a first plane, and the panoramic microscopic imaging module is connected to the translation bracket; wherein the first plane is parallel to the plane where the stage is located; the panoramic microscopic imaging module includes a panoramic image acquisition unit and a microscopic magnification unit; along the direction perpendicular to the stage, the effective working distance L1 between the panoramic image acquisition unit and the stage satisfies 30mm≤L1≤100mm; the microscopic magnification unit at least includes a magnifying objective lens group, the magnifying objective lens group includes at least one objective lens, and the focal length f of the objective lens satisfies 2mm≤f≤10mm. By adopting the above technical scheme, the panoramic microscopy imaging module is driven to move in the first plane by the translation bracket, and real-time panoramic microscopy imaging is achieved based on the panoramic imaging function of the panoramic microscopy imaging module, thereby solving the technical problem that real-time panoramic microscopy imaging cannot be achieved in the prior art; and, in the embodiment of the present invention, the effective working distance L1 between the panoramic image acquisition unit and the stage in the panoramic microscopy imaging module is reasonably set to satisfy 30mm≤L1≤100mm, and the focal length f of the objective lens satisfies 2mm≤f≤10mm. Under the premise of ensuring clear panoramic imaging, the panoramic microscopy imaging module is small and compact, thereby increasing the integration and portability of the portable panoramic cell analysis system.

[0052] The above is the core idea of ​​the present invention. The technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] Specifically, Figure 2 is a schematic diagram of the structure of a portable panoramic cell analysis system provided by an embodiment of the present invention, Figure 3 is a schematic diagram of a top view of a magnifying objective lens assembly provided by an embodiment of the present invention, Figure 4 is a schematic diagram of a microscopic image in a microscopic imaging field of view provided by an embodiment of the present invention, such as Figure 2 , Figure 3 and Figure 4 As shown, the portable panoramic cell analysis system provided by the embodiment of the present invention includes a translation bracket 10, a panoramic microscopic imaging module 20, a stage 30 and a light source 40; the translation bracket 10 can translate in a first plane, and the panoramic microscopic imaging module 20 is connected to the translation bracket 10; wherein the first plane is parallel to the plane where the stage 30 is located; the panoramic microscopic imaging module 20 includes a panoramic image acquisition unit 21 and a microscopic magnification unit 22; along the direction perpendicular to the stage 30, the effective working distance L1 between the panoramic image acquisition unit 21 and the stage 30 satisfies 30mm≤L1≤100mm; the microscopic magnification unit at least 22 includes a magnifying objective lens group 221, the magnifying objective lens group 221 includes at least one objective lens 2211, and the focal length f of the objective lens 2211 satisfies 2mm≤f≤10mm.

[0054] contrast Figure 1 and Figure 2 It can be seen that, different from the solution of translating the sample plate by a translation stage in the prior art, the embodiment of the present invention creatively sets a translation bracket 10 and a panoramic microscopic imaging module 20, and the panoramic microscopic imaging module 20 is connected to the translation bracket 10. The translation bracket 10 can move in a plane parallel to the plane where the stage 30 is located, thereby driving the panoramic microscopic imaging module 20 to move in a plane parallel to the plane where the stage 30 is located. Since the panoramic microscopic imaging module 20 can realize real-time panoramic imaging, the technical solution of the embodiment of the present invention can realize real-time panoramic microscopic imaging with good real-time performance. In addition, the technical solution of the embodiment of the present invention does not need to move the stage, thereby avoiding the influence of the observed sample due to the movement of the stage, and improving the imaging effect of panoramic microscopic imaging.

[0055] Further, in the technical solution of the embodiment of the present invention, along the direction perpendicular to the stage 30 (i.e., the X direction shown in the figure), the effective working distance L1 between the panoramic image acquisition unit 21 and the stage 30 satisfies 30mm≤L1≤100mm, the microscopic magnification unit 221 includes at least one objective lens 2211, and the focal length f of the objective lens 2211 satisfies 2mm≤f≤10mm. Within the effective working distance of the panoramic image acquisition unit 21 and the focal length range of the objective lens 1211, clear microscopic imaging can be performed on the observed samples in a large size range of 1μm≤L2≤120μm, such as Figure 3The solution of the embodiment of the present invention is conducive to reducing the size of the microscopic magnification unit 22, ensuring that the overall structure of the panoramic microscopic imaging module 20 is small and compact, and increasing the integration and portability of the portable panoramic cell analysis system.

[0056] Optional, continue to refer to Figure 2 As shown, the portable panoramic cell analysis system provided by the embodiment of the present invention also includes a stage 30 and a light source 40, and the stage 30 is used to carry the observed sample. The light source 40 can be a bright field light source, which is used to illuminate the observed sample, so that the panoramic microscopic imaging module 20 can perform panoramic microscopic imaging on the observed sample. Optionally, the light source 40 can include at least one of an LED light source, an OLED light source, or a laser light source, and the embodiment of the present invention does not limit the specific form of the light source 40.

[0057] In summary, the portable panoramic cell analysis system provided by the embodiment of the present invention is provided with a translation bracket and a panoramic microscopic imaging module, the panoramic microscopic imaging module is connected to the translation bracket, and the translation bracket can move in a plane parallel to the plane where the stage is located, thereby driving the panoramic microscopic imaging module to move in a plane parallel to the plane where the stage is located. Since the panoramic microscopic imaging module can realize real-time panoramic imaging, the technical solution of the embodiment of the present invention can realize real-time panoramic microscopic imaging with good real-time performance; at the same time, along the direction perpendicular to the stage, the effective working distance L1 between the panoramic image acquisition unit and the stage satisfies 30mm≤L1≤100mm, and the microscopic magnification unit includes at least one objective lens, and the focal length f of the objective lens satisfies 2mm≤f≤10mm; within such an effective working distance and such a focal length range, clear microscopic panoramic imaging can be performed on observation samples in a large size range satisfying 1μm≤L2≤120μm, thereby ensuring that the overall structure of the panoramic microscopic imaging module is small and compact, thereby increasing the integration and portability of the portable panoramic cell analysis system.

[0058] Figure 5 is a structural schematic diagram of a panoramic image acquisition unit provided by an embodiment of the present invention, such as Figure 5 As shown, the panoramic image acquisition unit 21 provided in the embodiment of the present invention may include a photoelectric conversion subunit 211, and the photoelectric conversion subunit 211 is electrically connected to an external image processing device.

[0059] Exemplarily, the panoramic image acquisition unit 21 provided in the embodiment of the present invention may only include a photoelectric conversion unit 211, and the photoelectric conversion unit 211 is used to convert the received optical signal into an electrical signal, and transmit the electrical signal to an external image processing module (not shown in the figure), and the external image processing module performs image processing based on the received electrical signal to obtain a panoramic microscopic image of the observed sample. The panoramic image acquisition unit 21 provided in the embodiment of the present invention is different from the CCD or CMOS structure in the prior art. The panoramic image acquisition unit 21 provided in the embodiment of the present invention may only include a photoelectric conversion subunit 211. The panoramic image acquisition unit 21 has a simple structure, which is conducive to reducing the volume of the portable panoramic cell analysis system and reducing the cost of the portable panoramic cell analysis system.

[0060] Furthermore, the panoramic image acquisition unit 21 provided in the embodiment of the present invention can be a panoramic imaging camera or a mobile terminal with a panoramic camera. The panoramic imaging camera or the mobile terminal with a panoramic camera, combined with at least one objective lens, can realize panoramic microscopic imaging, thereby ensuring that the portable panoramic cell analysis system has a simple structure and high practicality.

[0061] Optionally, the size of the panoramic image acquisition unit 22 is less than or equal to 1 / 3 inch.

[0062] For example, the chip size of CCD or CMOS is getting bigger and bigger, which makes the cost of scientific research-grade CCD or CMOS cameras also high. The portable panoramic cell analysis system provided by the embodiment of the present invention is different from the traditional CCD or CMOS camera in that a camera with a smaller panoramic image acquisition unit 22 size is used to achieve microscopic panoramic imaging in the field of cell analysis. For example, a panoramic image acquisition unit 22 with a lower cost of 1 / 3 inch photosensitive size is used to achieve a better imaging effect, which meets the use requirements of end customers and greatly reduces the purchase and use costs of end customers.

[0063] On the basis of the above-mentioned embodiment, the panoramic microscopic imaging module 20 is detachably arranged.

[0064] For example, different from the structure in which the microscopic imaging module is fixedly integrated in the existing CCD or CMOS structure, in the portable panoramic cell analysis system provided by the embodiment of the present invention, the panoramic microscopic imaging module 20 is detachably arranged. Specifically, the panoramic microscopic imaging module 20 can be detachably arranged on a supporting column (not shown in the figure), so that the user can select different panoramic microscopic imaging modules 20 according to different observed samples. For example, when performing high-magnification microscopic imaging and low-magnification microscopic imaging on the observed samples, different microscopic imaging modules can be directly replaced based on the detachable performance of the microscopic imaging module, and each microscopic imaging module only needs fine-tuning to achieve clear imaging, thereby ensuring that clear imaging can be achieved simply and quickly under different magnification requirements.

[0065] Figure 6 is a schematic diagram of another portable panoramic cell analysis system provided by an embodiment of the present invention, Figure 2 and Figure 6 As shown, the portable panoramic cell analysis system provided by the embodiment of the present invention may further include a lifting assembly 50, on which a translation bracket 10 is disposed, and a panoramic microscopic imaging module 20 is detachably disposed on the translation bracket 10, and the lifting assembly 50 may drive the translation bracket 10 to move, thereby realizing different working states of the portable panoramic cell analysis system.

[0066] Specifically, Figure 2 As shown, when the portable panoramic cell analysis system is in working state, the panoramic microscopic imaging module 20 is disposed on a side of the stage 30 away from the light source 40 .

[0067] Exemplarily, the portable panoramic cell analysis system being in working state can be understood as the portable panoramic cell analysis system being in normal panoramic microscopic imaging state. When the portable panoramic cell analysis system is in normal working state, the panoramic image acquisition unit 21 normally receives the optical signal, converts the optical signal into an electrical signal, and performs panoramic imaging at the same time. At this time, the lifting assembly 50 rises, and at the same time drives the translation bracket 10 and the panoramic microscopic imaging module 20 to rise, and is set in the direction perpendicular to the stage 30 (such as the X direction described in the figure). The distance L2 between the panoramic image acquisition unit 21 and the stage 30 satisfies 30mm≤L2≤100mm. Under the premise of satisfying the normal panoramic imaging of the panoramic image acquisition unit 21, the volume of the panoramic microscopic imaging module 20 is reduced, and the volume of the portable panoramic cell analysis system is further reduced, which is in line with the development trend of miniaturization and portability of the portable panoramic cell analysis system.

[0068] Figure 7 is a structural schematic diagram of a panoramic microscopic imaging module provided by an embodiment of the present invention, Figure 8 is a schematic diagram of a top view of a stage provided by an embodiment of the present invention, such as Figure 6 , Figure 7 and Figure 8 As shown, an opening 31 is formed on the stage 30; the panoramic microscopic imaging module 20 includes a first portion 201 close to a side of the stage 30 and a second portion 202 away from a side of the stage 30, and a vertical projection of the first portion 201 on the plane where the stage 30 is located is located in the opening 31; when the portable panoramic cell analysis system is in a non-working state, the first portion 201 is nested in the opening 31.

[0069] Exemplarily, as shown in FIG7 , the panoramic microscopic imaging module 20 includes a first part 201 close to the stage 30 and a second part 202 away from the stage 30, wherein the first part 201 may be a microscopic magnification unit 22, and the second part 202 may be a panoramic image acquisition unit 21; or the first part 201 may be a part of the microscopic magnification unit 22, and the second part 202 may include a panoramic image acquisition unit 21 and a part of the microscopic magnification unit 22; or the first part 201 may include a microscopic magnification unit 22 and a part of the panoramic image acquisition unit 21, and the second part 202 may include a part of the panoramic image acquisition unit 21. The embodiment of the present invention does not limit the composition of the first part 201 and the second part 202. Figure 7 The first part 201 is a microscopic magnification unit 22, and the second part 302 is a panoramic image acquisition unit 21. Figure 6 and Figure 8 As shown, an opening 31 is formed on the stage 30, and the vertical projection of the first part 201 on the plane where the stage 30 is located is located in the opening 31. In this way, when the portable panoramic cell analysis system is in a non-working state, the panoramic microscopic imaging module 20 can be driven to descend to the side close to the stage 30 by the lifting assembly 50, so that the first part 201 of the panoramic microscopic imaging module 20 is nested in the opening 31, thereby ensuring that the portable panoramic cell analysis system is small in size when it is not in a working state, and is convenient for storage and moving; and, when the portable panoramic cell analysis system is set in a non-working state, the first part 201 of the panoramic microscopic imaging module 20 is nested in the opening 31, which can also protect the panoramic microscopic imaging module 20 and prevent the panoramic microscopic imaging module 20 from being damaged by external hard objects.

[0070] Optionally, along the direction perpendicular to the stage 30 , the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22 is adjustable.

[0071] For example, in addition to setting the panoramic microscopic imaging module 20 and the translation bracket 10 to be detachably connected, different magnifications can be achieved by replacing the panoramic microscopic imaging module 20 as a whole, ensuring that clear panoramic imaging can be achieved simply and quickly under different magnification requirements. In addition, the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22 can be set to be adjustable. By adjusting the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22, the magnification can be changed without changing the optical resolution. This can be applied to observation or detection scenes that require image magnification at the same optical resolution. In addition, unlike the fixed magnification switching of traditional microscopes, the embodiment of the present invention can achieve continuous magnification switching by setting the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22 to be adjustable, and by gradually adjusting the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22.

[0072] It should be noted that the above embodiment is described by taking the portable panoramic cell analysis system as an upright cell analysis system as an example, that is, in order from top to bottom, the portable panoramic cell analysis system includes a panoramic microscopic imaging module 20, a stage 30 and a light source 40 in sequence. It can be understood that the portable panoramic cell analysis system provided in the embodiment of the present invention can also be an inverted cell analysis system, that is, in order from top to bottom, the portable panoramic cell analysis system includes a light source 40, a stage 30 and a panoramic microscopic imaging module 20 in sequence. The inverted portable panoramic cell analysis system is described in detail below.

[0073] Optionally, the light source 40 is detachable.

[0074] Exemplarily, the light source 40 is detachably arranged, and the light source can be replaced simply and quickly according to different light source requirements. For example, when performing microscopic imaging on a large-area observation sample, a large-area light source or a wide-angle light source can be replaced to ensure that the large-area observation sample is in the bright field area; when performing microscopic imaging on a small-area observation sample, a small-area light source can be replaced, which is beneficial to energy saving and environmental protection.

[0075] Based on the above embodiments, Fig. 9 is a schematic diagram of the structure of another portable panoramic cell analysis system according to an embodiment of the present invention, Fig.10 FIG. 1 is a schematic diagram of the structure of another portable panoramic cell analysis system provided by an embodiment of the present invention. Fig. 9 and Fig.10 As shown, the portable cell analysis system provided by the embodiment of the present invention may further include a lifting assembly 50, on which the light source 40 is detachably mounted. The lifting assembly 50 may drive the light source 40 to move, and bright field areas of different areas may be formed according to the requirements of microscopic panoramic imaging, which is beneficial to improving the clarity of microscopic panoramic imaging.

[0076] For further information, please refer to Fig. 9 As shown, when the portable panoramic cell analysis system is in working state, the light source 40 is arranged on the side of the stage 30 away from the panoramic microscopic imaging module 20, and the light source 40 is driven to move by the lifting assembly 50, thereby ensuring that bright field areas of different areas can be formed according to the requirements of microscopic panoramic imaging, which is beneficial to improving the clarity of microscopic panoramic imaging.

[0077] Furthermore, Fig.10 is a front view of another portable panoramic cell analysis system provided by an embodiment of the present invention, such as Figure 8 and Fig.10 As shown, an opening 31 is formed on the stage 30; the vertical projection of the light source 40 on the plane where the stage 30 is located is located in the opening 31; when the portable panoramic cell analysis system is in a non-working state, the light source 40 is nested in the opening 31. In this way, when the portable panoramic cell analysis system is in a non-working state, the light source 40 can be driven by the lifting assembly 50 to descend to the side close to the stage 30 so that the light source 40 is nested in the opening 31, thereby ensuring that the portable panoramic cell analysis system is small in size when it is not in a working state, and is convenient for storage and movement; and, when the portable panoramic cell analysis system is in a non-working state, the light source 40 is nested in the opening 31 to protect the light source 40 and prevent the light source 40 from being damaged by external hard objects.

[0078] Optionally, when the portable panoramic cell analysis system is an inverted cell analysis system, the distance between the panoramic image acquisition unit 21 and the microscope magnification unit 22 along the direction perpendicular to the stage 30 can also be adjusted.

[0079] Exemplarily, the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22 is set to be adjustable. By adjusting the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22, the magnification ratio can be changed without changing the optical resolution, which can be applied to observation or detection scenes that require image magnification at the same optical resolution. In addition, unlike the fixed magnification switching of traditional microscopes, the embodiment of the present invention sets the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22 to be adjustable. By gradually adjusting the distance between the panoramic image acquisition unit 21 and the microscopic magnification unit 22, continuous magnification switching can be achieved.

[0080] Further, when the portable panoramic cell analysis system is an inverted cell analysis system, the panoramic microscopic imaging module 20 can be a detachable structure. Specifically, the panoramic microscopic imaging module 20 can be detachably arranged on a support column (not shown in the figure), so that the user can select different panoramic microscopic imaging modules 20 according to different observation samples. For example, when performing high-magnification microscopic imaging and low-magnification microscopic imaging on the observation samples, different panoramic microscopic imaging modules can be directly replaced based on the detachable performance of the microscopic imaging module. Each panoramic microscopic imaging module only needs fine-tuning to achieve clear imaging, ensuring that clear panoramic imaging can be achieved simply and quickly under different magnification requirements.

[0081] On the basis of the above-mentioned embodiment, the microscopic magnification unit 22 provided in the embodiment of the present invention may have a plurality of different magnification ratios. Next, how the microscopic magnification unit realizes a plurality of different magnification ratios is described in detail.

[0082] Optional, continue to refer to Figure 3 As shown, the magnifying objective lens group 221 provided in the embodiment of the present invention may include a plurality of objective lenses 2211 with different magnifications.

[0083] For example, Figure 3 As shown, the magnifying objective lens group 221 provided in the embodiment of the present invention may include an objective lens turret 2212 and a plurality of objective lenses 2211 with different magnifications arranged on the objective lens turret 2212. By rotating the objective lens turret 1212, the objective lenses 2211 with different magnifications are replaced, and objective lenses 2211 with different magnifications are selected under different magnification requirements, thereby ensuring that the panoramic microscopic imaging module 20 can be applicable to situations with different magnification requirements, thereby improving the universality of the portable panoramic cell analysis system.

[0084] Optional, Fig.11 FIG. 1 is a schematic diagram of a top view structure of a magnifying eyepiece assembly provided by an embodiment of the present invention. Fig.11 As shown, the microscope magnification unit 22 provided in the embodiment of the present invention may further include a magnifying eyepiece group 222, which is located on the side of the magnifying objective lens group 221 away from the stage 30; the magnifying eyepiece group 222 may include a plurality of eyepieces 2221 with different magnifications.

[0085] For example, by adding a magnifying eyepiece set 222 to the microscopic magnification unit 22, and arranging the magnifying eyepiece set 222 to be located on the side of the magnifying objective lens set 221 away from the stage 30, the magnification of the microscopic magnification unit 22 can be further adjusted by the magnifying eyepiece set 222, thereby improving the panoramic microscopic imaging effect of the portable panoramic cell analysis system. Fig.11As shown, the magnifying eyepiece set 222 may include an eyepiece turntable 2222, and a plurality of eyepieces 2221 with different magnifications are arranged on the eyepiece turntable 2222. The eyepieces 2221 with different magnifications are replaced by rotating the eyepiece turntable 2222. The magnifying eyepiece set 222 may include a plurality of eyepieces 2221 with different magnifications. For different magnification requirements, the flexibility of magnification adjustment can be increased by adjusting the eyepieces 2221 with different magnifications.

[0086] It should be noted that Fig.11 The magnifying eyepiece assembly 222 is described as a feasible manner rather than being a limitation on the magnifying eyepiece assembly 222. It is understandable that other compositions of the magnifying eyepiece assembly 222 are also within the protection scope of the embodiment of the present invention.

[0087] Optionally, in the portable panoramic cell analysis system provided by the embodiment of the present invention, the magnifying objective lens group may include multiple objective lenses with different magnifications; the micro-magnification unit may also include a magnifying eyepiece group, which may include multiple eyepieces with different magnifications; an eyepiece and an objective lens may form a combined lens. Specifically, Fig.12 is a schematic diagram of the structure of a combined lens provided by an embodiment of the present invention, such as Fig.12 As shown, an eyepiece 2221 and an objective lens 2211 form a combined lens 223, and the combined lens 223 is used to combine and magnify the observed sample to improve the magnification effect of the observed sample.

[0088] In summary, in the microscopic magnification unit provided by the embodiment of the present invention, the magnifying objective lens group may include multiple objective lenses with different magnifications; and / or, the microscopic magnification unit may also include a magnifying eyepiece group, and the magnifying eyepiece group includes multiple eyepieces with different magnifications. Furthermore, the eyepiece and the objective lens may also form a combined lens, and through objective lenses with different magnifications, and / or, through eyepieces with different magnifications, and / or, through combined lenses with different magnifications, the requirements of different magnifications are met, thereby ensuring that the portable panoramic cell analysis system provided by the embodiment of the present invention has good universality.

[0089] Based on the above embodiments, the portable panoramic cell analysis system provided by the embodiments of the present invention may further include a control module (not shown in the figure), which is connected to the microscope magnification unit and is used to adjust the magnification of the microscope magnification unit according to the size of the observed sample or based on the user's magnification requirements.

[0090] Exemplarily, according to the size of the observed sample, the user can manually adjust the control module to realize the microscopic magnification imaging of the sample to be measured by using objective lenses with different magnifications, eyepieces with different magnifications, and / or combined lenses with different magnifications; or, the control module receives the user's magnification requirements and automatically controls and adjusts according to the user's magnification requirements to realize the microscopic magnification imaging of the sample to be measured by using objective lenses with different magnifications, eyepieces with different magnifications, and / or combined lenses with different magnifications. The embodiment of the present invention does not limit the control module to realize the magnification switching.

[0091] Optional, Fig.13 is a schematic diagram of the structure of another panoramic image acquisition unit provided by an embodiment of the present invention. Fig.13 As shown, the panoramic image acquisition unit 21 may further include at least one magnifying lens 213. Fig.13 The panoramic image acquisition unit 21 including a magnifying lens 213 is taken as an example for description.

[0092] Exemplarily, by adding at least one magnifying lens 213 to the panoramic image acquisition unit 21 , the magnification effect of the portable panoramic cell analysis system is further improved.

[0093] Based on the same inventive concept, the embodiment of the present invention also provides a panoramic microscopic imaging method, using the portable panoramic cell analysis system provided by the embodiment of the present invention, such as Fig.14 As shown, the panoramic imaging microscopy method provided by the embodiment of the present invention includes:

[0094] S110, determining a panoramic microscopic imaging path.

[0095] S120, controlling the translation support to drive the panoramic microscopic imaging module to move according to the panoramic microscopic imaging path, so as to obtain a panoramic microscopic image of the observed sample.

[0096] Exemplarily, the panoramic microscopic imaging path can be understood as the moving path of the panoramic microscopic imaging module, and the moving path can be "U" shaped or "Z" shaped. The embodiment of the present invention does not limit the specific path shape. According to the determined panoramic microscopic imaging path, the translation bracket is controlled to drive the panoramic microscopic imaging module to move, and a panoramic microscopic image of the observed sample is obtained in real time.

[0097] Optionally, before determining the panoramic microscopy imaging path, the following may also be included:

[0098] Add the pre-processed observation sample to the stage;

[0099] Turn on the light source to stimulate the optical signal to be collected;

[0100] The magnification of the microscope magnification unit is adjusted so that the sample image observed by the panoramic image acquisition unit is clear.

[0101] In summary, the panoramic microscopic imaging method provided by the embodiment of the present invention first determines the panoramic microscopic imaging path, and then controls the translation bracket to move in a plane parallel to the plane where the stage is located according to the path, thereby driving the panoramic microscopic imaging module to move in a plane parallel to the plane where the stage is located, and realizes real-time panoramic imaging based on the panoramic imaging function of the panoramic microscopic imaging module. The panoramic imaging method is simple and fast; at the same time, there is no need to move the stage, which avoids affecting the observed sample due to the movement of the stage, thereby improving the imaging effect of panoramic microscopic imaging.

[0102] On the basis of the above-mentioned embodiment, controlling the translation support to drive the panoramic microscopic imaging module to move according to the panoramic microscopic imaging path to obtain a panoramic microscopic image of the observed sample may include:

[0103] Continuously adjusting the distance between the panoramic image acquisition unit and the microscopic magnification unit to a preset distance along a direction perpendicular to the stage;

[0104] A portable panoramic cell analysis system is used to perform microscopic imaging of a single frame of the observed sample at a preset distance;

[0105] The translation bracket is controlled according to the panoramic microscopic imaging path to drive the panoramic microscopic imaging module to move, so as to obtain a panoramic microscopic image of the observed sample.

[0106] Exemplarily, the distance between the panoramic image acquisition unit and the microscopic magnification unit can be continuously adjusted. During the panoramic microscopic imaging process, the distance between the panoramic image acquisition unit and the microscopic magnification unit can be continuously adjusted according to the size of the observed sample or user needs, so as to obtain a clear magnified image of the observed sample or meet the user's magnification needs for a single imaging screen. At this time, the distance between the panoramic image acquisition unit and the microscopic magnification unit can be understood as a preset distance; at the above-mentioned preset distance, the portable panoramic cell analysis system provided by the embodiment of the present invention is used to perform microscopic imaging on the observed sample to ensure that a clear microscopic magnified image for a single screen is obtained; then, according to the determined panoramic microscopic imaging path, the translation bracket is controlled to drive the panoramic microscopic imaging module to move, and a panoramic microscopic image of the observed sample is obtained in real time.

[0107] The microscopic imaging method provided in the embodiment of the present invention can realize continuous switching of magnification by continuously adjusting the distance between the image acquisition unit and the microscopic magnification unit, thereby ensuring that the clearest magnified image of the observed sample is obtained or meeting the user's magnification requirements. The microscopic imaging method is simple, accurate and highly practical.

[0108] Note that the above are only preferred embodiments of the present invention and the technical principles used. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and the features of the various embodiments of the present invention may be partially or completely coupled or combined with each other, and may cooperate with each other in various ways and be technically driven. It is possible for those skilled in the art to make various obvious changes, readjustments, combinations and substitutions without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in more detail through the above embodiments, the present invention is not limited to the above embodiments, and may include more other equivalent embodiments without departing from the concept of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A portable panoramic cell analysis system, characterized in that: It includes a translation bracket, a panoramic microscopic imaging module, a stage and a light source; the light source includes at least one of an LED light source, an OLED light source or a laser light source; The translation bracket can translate in a first plane, and the panoramic microscopic imaging module is connected to the translation bracket; wherein the first plane is parallel to the plane where the stage is located; The panoramic microscopic imaging module includes a panoramic image acquisition unit and a microscopic magnification unit; along the direction perpendicular to the stage, the effective working distance L1 between the panoramic image acquisition unit and the stage satisfies 30mm≤L1≤100mm; the microscopic magnification unit at least includes a magnifying objective lens group, the magnifying objective lens group includes at least one objective lens, and the focal length f of the objective lens satisfies 2mm≤f≤10mm; The portable panoramic cell analysis system further comprises a lifting component, the translation support is arranged on the lifting component, and the panoramic microscopic imaging module is detachably arranged on the translation support; When the portable panoramic cell analysis system is in working state, the panoramic microscopic imaging module is arranged on a side of the stage away from the light source; An opening is formed on the stage; the panoramic microscopic imaging module includes a first part close to one side of the stage and a second part away from the side of the stage, and the vertical projection of the first part on the plane where the stage is located is located in the opening; when the portable panoramic cell analysis system is in a non-working state, the first part is nested in the opening.

2. The portable panoramic cell analysis system according to claim 1, characterized in that: The panoramic image acquisition unit includes a photoelectric conversion subunit, and the photoelectric conversion subunit is electrically connected to an external image processing device.

3. The portable panoramic cell analysis system according to claim 1, characterized in that: The panoramic image acquisition unit includes a panoramic imaging camera.

4. The portable panoramic cell analysis system according to claim 1, characterized in that: Along a direction perpendicular to the stage, the distance between the panoramic image acquisition unit and the microscopic magnification unit is adjustable.

5. The portable panoramic cell analysis system according to any one of claims 1 to 3, characterized in that: The magnifying objective lens group includes a plurality of objective lenses with different magnifications.

6. The portable panoramic cell analysis system according to any one of claims 1 to 3, characterized in that: The microscope magnification unit further comprises a magnifying eyepiece set, and the magnifying eyepiece set is located on a side of the magnifying objective lens set away from the stage; The magnifying eyepiece set includes a plurality of eyepieces with different magnifications.

7. The portable panoramic cell analysis system according to any one of claims 1 to 3, characterized in that: The magnifying objective lens group includes a plurality of objective lenses with different magnifications; The microscopic magnification unit further includes a magnifying eyepiece set, wherein the magnifying eyepiece set includes a plurality of eyepieces with different magnifications; One of the eyepieces and one of the objective lenses form a combined lens.

8. The portable panoramic cell analysis system according to any one of claims 1 to 3, characterized in that: The size of the panoramic image acquisition unit is less than or equal to 1 / 3 inch.

9. A portable panoramic cell analysis system, characterized in that: It includes a translation bracket, a panoramic microscopic imaging module, a stage and a light source; the light source includes at least one of an LED light source, an OLED light source or a laser light source; The translation bracket can translate in a first plane, and the panoramic microscopic imaging module is connected to the translation bracket; wherein the first plane is parallel to the plane where the stage is located; The panoramic microscopic imaging module includes a panoramic image acquisition unit and a microscopic magnification unit; along the direction perpendicular to the stage, the effective working distance L1 between the panoramic image acquisition unit and the stage satisfies 30mm≤L1≤100mm; the microscopic magnification unit at least includes a magnifying objective lens group, the magnifying objective lens group includes at least one objective lens, and the focal length f of the objective lens satisfies 2mm≤f≤10mm; The portable panoramic cell analysis system further comprises a lifting component, and the light source is detachably arranged on the lifting component; When the portable panoramic cell analysis system is in working state, the light source is arranged on a side of the stage away from the panoramic microscopic imaging module; An opening is formed on the stage; a vertical projection of the light source on the plane where the stage is located is located in the opening; when the portable panoramic cell analysis system is in a non-working state, the light source is nested in the opening.

10. A panoramic microscopic imaging method of a portable panoramic cell analysis system, using the portable panoramic cell analysis system according to any one of claims 1 to 9, characterized in that: include: Determine the panoramic microscopy imaging path; The translation support is controlled according to the panoramic microscopic imaging path to drive the panoramic microscopic imaging module to move, so as to obtain a panoramic microscopic image of the observed sample.

11. The panoramic microscopic imaging method according to claim 10, characterized in that: Controlling the translation support to drive the panoramic microscopic imaging module to move according to the panoramic microscopic imaging path to obtain a panoramic microscopic image of the observed sample, including: Continuously adjusting the distance between the panoramic image acquisition unit and the microscopic magnification unit to a preset distance along a direction perpendicular to the stage; At the preset distance, using the portable panoramic cell analysis system to perform microscopic imaging on a single screen of the observed sample; The translation support is controlled according to the panoramic microscopic imaging path to drive the panoramic microscopic imaging module to move, so as to obtain a panoramic microscopic image of the observed sample.

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