Rapid extraction tool for nucleic acid in nasopharyngeal swab samples and method of use thereof

Abstract

The present invention discloses a rapid extraction tool for nucleic acid in nasopharyngeal swab samples and a method for using the same, and relates to the technical field of nucleic acid detection. The invention aims to solve the problem that the existing collection tool and collection of nasopharyngeal swabs are divided into two parts, which is inconvenient to use, and is not sealed enough during the collection and storage process, resulting in susceptibility to external infection. A frosted belt is provided in the first storage box, a first connecting ring is provided in the first storage box, a second connecting ring is provided at the lower end of the first connecting ring, a second storage box is provided in the second connecting ring, a plug-in card is provided in the second storage box, a test rod is provided in the first storage box, a sampling head is provided at the upper end of the test rod, a rubber layer is provided in the first connecting ring, an opening is provided in the rubber layer, and the opening is connected to the upper end of the test rod by snapping.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a rapid extraction tool for nucleic acid in nasopharyngeal swab samples and a method for using the tool. Background Art

[0002] The substance of nucleic acid testing is the nucleic acid of the virus. Nucleic acid testing is to find out whether there is the nucleic acid of foreign invading viruses in the patient's respiratory specimens, blood or feces to determine whether the person is infected with the new coronavirus. After the new coronavirus infects the human body, it will first reproduce in the respiratory system. Therefore, it is possible to determine whether the human body is infected with the virus by detecting viral nucleic acids in sputum and nasopharyngeal swabs.

[0003] During the nucleic acid test, a special nasopharyngeal swab is needed to collect samples for the convenience of medical staff to conduct testing. The existing nasopharyngeal swab collection tool and collection are divided into two parts, which is inconvenient to use. In addition, the collection and storage process is not sealed enough, making it easy to be infected by the outside world. Summary of the invention

[0004] The object of the present invention is to provide a rapid extraction tool for nucleic acid in nasopharyngeal swab samples and a method for using the same, so as to solve the problem that the collection tool and collection of nasopharyngeal swabs proposed in the above background technology are divided into two parts, which causes certain inconveniences during use, and the collection and storage process is not sealed enough, resulting in susceptibility to external infection.

[0005] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: a tool for rapid extraction of nucleic acids in nasopharyngeal swab samples and a method of using the same, comprising a first storage box, a frosted belt is provided in the first storage box, a first connecting ring is provided in the first storage box, a second connecting ring is provided at the lower end of the first connecting ring, a second storage box is provided in the second connecting ring, an insertion card is provided in the second storage box, a test rod is provided in the first storage box, a sampling head is provided at the upper end of the test rod, a rubber layer is provided in the first connecting ring, an opening is provided in the rubber layer, and the opening is snap-connected with the upper end of the test rod.

[0006] Preferably, a first groove is provided in the second connecting ring, a first thread is provided at the lower end of the first connecting ring, the first thread is movably connected to the first groove, a second thread is provided in the second connecting ring, and a third thread is provided in the first connecting ring.

[0007] Preferably, a vacuum box is arranged in the first storage box, a first tin foil is arranged at the lower end of the vacuum box, and the vacuum box is movably connected to the sampling head.

[0008] Preferably, a first fixed edge is provided in the second storage box, a second tin foil is provided in the first fixed edge, and salt water is provided at the lower end of the second tin foil.

[0009] Preferably, a first slide groove is provided in the second storage box, the first slide groove is slidably connected to the card insertion, a second fixed edge is provided in the second storage box, the second fixed edge is fixedly connected to the first slide groove, and a symmetrical second slide groove is provided in the second fixed edge.

[0010] Preferably, symmetrical sliders are provided at the upper and lower ends of the plug-in card, symmetrical first magnetic layers are provided at the front ends of the two sliders, symmetrical second magnetic layers are provided in the two second slide grooves, and the two second magnetic layers are movably connected to the first magnetic layer.

[0011] Preferably, the front end of the card insertion unit is provided with a first sawtooth, and the first sawtooth is made of stainless steel.

[0012] Preferably, a third fixed edge is provided in the second storage box, the third fixed edge corresponds to the card insertion, a second groove is provided at the front end of the third fixed edge, a second serration is provided in the second groove, and a finger pressing groove is provided on one side of the card insertion.

[0013] The method for using a rapid nucleic acid extraction tool in a nasopharyngeal swab sample comprises the following steps:

[0014] Step 1: First, the first storage box and the second storage box are connected together by the first connecting ring and the second connecting ring set between them, and the first storage box is first removed by the third thread, and the sampling head is pulled out from the first tin foil through the test rod. The first tin foil can ensure the tight effect without affecting the removal during use, and the sampling head at the front end of the test rod is used to sample the patient, thereby improving the convenience and efficiency of using the nasopharyngeal swab;

[0015] Step 2: When sampling, connect the first storage box to the first connection ring and remove it from the second connection ring for centralized collection and destruction. After collecting the sample, remove the second connection ring from the second storage box, and pass the sampling head of the front end of the test rod downward through the second tin foil to immerse it in salt water. The second tin foil set here can prevent the salt water from leaking out before use, and also ensure that the salt water is not invaded by external bacteria before use, which will affect the detection structure;

[0016] Step 3: To ensure the personal safety of medical staff, reduce contact with the test rod and avoid improper operation to injure fingers when breaking the test rod to reduce the chance of infection, press the thumb on the finger pressure groove of the card, push the card inward to make the slider slide forward in the second slide groove to make the first magnetic layer and the second magnetic layer adsorbed, and the test rod can be cut off when the first serration at the front end of the card is engaged with the second serration on the third fixed edge. Also, because the first magnetic layer and the second magnetic layer of the slider of the card are adsorbed to each other, the second serration and the first serration can remain in a matching state after cutting, thereby preventing the saline 19 from leaking out to protect the sample and also preventing the virus from affecting the outside world.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The rapid extraction tool for nucleic acid in nasopharyngeal swab samples and the use method thereof, the frosted belt can be used to facilitate the removal of the first storage box, the first connecting ring and the second connecting ring can be used to connect the first storage box and the second storage box, the test rod can pass through the rubber layer in the first connecting ring, which is convenient for limiting and fixing, the third thread is used to facilitate the connection of the first connecting ring with the first storage box, the first connecting ring is fixedly connected to the first groove of the second connecting ring through the first thread at the lower end, thereby combining the first storage box and the second storage box into one, the vacuum box is used to ensure that the sampling head is not affected by the external environment before use, and the first tin foil is used to ensure the tight effect without affecting the removal during use.

[0019] 2. The rapid extraction tool for nucleic acid in nasopharyngeal swab samples and the use method thereof can ensure that the sampling head is not affected by the external environment before use by setting a vacuum box, and the first tin foil set can ensure a tight effect without affecting the removal during use, and the second tin foil set in the second storage box can prevent saline from leaking out before use.

[0020] 3. The rapid extraction tool for nucleic acid in nasopharyngeal swab samples and the use method thereof can slide inward in the second slide groove through the sliders arranged above and below the insertion card, so that the first iron-absorbing layer and the second iron-absorbing layer of the slider are adsorbed on each other, which is convenient for fixing the insertion card. The first serration arranged at the front end of the insertion card can facilitate the nasopharyngeal test sample to be placed in the second storage box for cutting after sampling, thereby reducing the contact between medical staff and the test rod.

[0021] 4. The rapid extraction tool for nucleic acid in nasopharyngeal swab samples and the use method thereof, by setting a third fixed edge in the second storage box, the test rod can penetrate the second tin foil with the sampling head facing down to immerse it in saline after use, and the second groove is convenient for the test rod to go deep, and at the same time, the second serration in the third fixed edge coincides with the first serration to facilitate cutting of the test rod, and because the first magnetic absorption layer and the second magnetic absorption layer of the slider of the card insertion are mutually adsorbed, the second serration and the first serration can remain in a coincident state after cutting, thereby preventing the saline 19 from leaking out, which plays a protective role for the sample and also prevents the virus from affecting the outside world. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0023] Figure 2 The cross-sectional structure of the present invention is shown in FIG. Figure 1 ;

[0024] Figure 3 The cross-sectional structure of the present invention is shown in FIG. Figure 2 ;

[0025] Figure 4 It is a schematic structural diagram of part A of the present invention;

[0026] Figure 5 It is a schematic structural diagram of part B of the present invention.

[0027] In the figure: 1. first storage box; 2. frosted belt; 3. first connecting ring; 4. second connecting ring; 5. second storage box; 6. card insertion; 7. test rod; 8. sampling head; 9. rubber layer; 10. opening; 11. first groove; 12. first thread; 13. second thread; 14. third thread; 15. vacuum box; 16. first tin foil; 17. first fixed edge; 18. second tin foil; 19. salt water; 20. first slide groove; 21. second fixed edge; 22. second slide groove; 23. slider; 24. first magnetic layer; 25. second magnetic layer; 26. first serration; 27. third fixed edge; 28. second groove; 29. ​​second serration; 30. finger pressure groove. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0029] See also Figure 1-5An embodiment of the present invention provides a rapid extraction tool for nucleic acid in a nasopharyngeal swab sample and a method of using the same, comprising a first storage box 1, a frosted belt 2 is provided in the first storage box 1, a first connecting ring 3 is provided in the first storage box 1, a second connecting ring 4 is provided at the lower end of the first connecting ring 3, a second storage box 5 is provided in the second connecting ring 4, a plug-in card 6 is provided in the second storage box 5, a test rod 7 is provided in the first storage box 1, a sampling head 8 is provided at the upper end of the test rod 7, a rubber layer 9 is provided in the first connecting ring 3, an opening 10 is provided in the rubber layer 9, and the opening 10 is engaged and connected with the upper end of the test rod 7.

[0030] Furthermore, a first groove 11 is provided in the second connecting ring 4, a first thread 12 is provided at the lower end of the first connecting ring 3, the first thread 12 is movably connected to the first groove 11, a second thread 13 is provided in the second connecting ring 4, and a third thread 14 is provided in the first connecting ring 3. The frosted belt 2 can be used to facilitate the removal of the first storage box 1. The first connecting ring 3 and the second connecting ring 4 can play a role in connecting the first storage box 1 and the second storage box 5. The test rod 7 can pass through the rubber layer 9 in the first connecting ring 3 to facilitate the limiting and fixing. The third thread 14 facilitates the connection between the first connecting ring 3 and the first storage box 1. The first connecting ring 3 is fixedly connected to the first groove 11 of the second connecting ring 4 through the first thread 12 at the lower end, thereby combining the first storage box 1 and the second storage box 5 into one.

[0031] Furthermore, a vacuum box 15 is arranged in the first storage box 1, and a first tin foil 16 is arranged at the lower end of the vacuum box 15. The vacuum box 15 is movably connected to the sampling head 8. By arranging the vacuum box 15, it can be ensured that the sampling head 8 is not affected by the external environment before use, and the first tin foil 16 can ensure the tight effect without affecting the removal during use.

[0032] Furthermore, a first fixed edge 17 is provided in the second storage box 5, a second tin foil 18 is provided in the first fixed edge 17, and salt water 19 is provided at the lower end of the second tin foil 18. The second tin foil 18 provided in the second storage box 5 can prevent the salt water 19 from leaking out before use.

[0033] Furthermore, a first slide groove 20 is provided in the second storage box 5, and the first slide groove 20 is slidably connected to the card insertion 6. A second fixed edge 21 is provided in the second storage box 5, and the second fixed edge 21 is fixedly connected to the first slide groove 20. A symmetrical second slide groove 22 is provided in the second fixed edge 21. Through the first slide groove 20 provided in the second storage box 5, the card insertion 6 can slide in the second slide groove 22.

[0034] Furthermore, symmetrical sliders 23 are provided at the upper and lower ends of the plug-in card 6, and symmetrical first magnetic layers 24 are provided at the front ends of the two sliders 23. Symmetrical second magnetic layers 25 are provided in the two second slide grooves 22. The two second magnetic layers 25 are movably connected to the first magnetic layers 24. The sliders 23 provided at the upper and lower ends of the plug-in card 6 can slide inward in the second slide grooves 22, so that the first magnetic layer 24 and the second magnetic layer 25 of the slider 23 are adsorbed on each other, which is convenient for fixing the plug-in card 6.

[0035] Furthermore, a first serration 26 is provided at the front end of the insertion card 6. The first serration 26 is made of stainless steel. The first serration 26 provided at the front end of the insertion card 6 can facilitate the nasopharyngeal swab to be placed in the second storage box 5 for cutting after sampling, thereby reducing the contact between medical staff and the test rod 7.

[0036] Furthermore, a third fixed edge 27 is provided in the second storage box 5, and the third fixed edge 27 corresponds to the insertion card 6. A second groove 28 is provided at the front end of the third fixed edge 27, and a second serration 29 is provided in the second groove 28. A finger pressing groove 30 is provided on one side of the insertion card 6. By providing the third fixed edge 27 in the second storage box 5, the test rod 7 can penetrate the second tin foil 18 with the sampling head 8 facing downward to immerse it in the salt water 19 after use. The second groove 28 facilitates the penetration of the test rod 7. At the same time, the second serration 29 in the third fixed edge 27 coincides with the first serration 26 to facilitate cutting off the test rod 7. Also, because the first magnetic layer 24 and the second magnetic layer 25 of the slider 23 of the insertion card 6 are mutually adsorbed, the second serration 29 and the first serration 26 can remain in a coincident state after cutting, thereby preventing the salt water 19 from leaking out, which plays a protective role for the sample and also prevents the virus from affecting the outside world.

[0037] The method for using a rapid nucleic acid extraction tool in a nasopharyngeal swab sample comprises the following steps:

[0038] Step 1: First, the first storage box 1 and the second storage box 5 are connected together by the first connecting ring 3 and the second connecting ring 4 set between them. The first storage box 1 is first removed through the third thread 14, and the sampling head 8 is pulled out from the first tin foil 16 through the test rod 7. The vacuum box 15 can ensure that the sampling head 8 is not affected by the external environment before use, and the first tin foil 16 can ensure the tight effect without affecting the removal during use. The sampling head 8 at the front end of the test rod 7 is used to sample the patient, thereby improving the convenience and efficiency of using the nasopharyngeal swab;

[0039] Step 2: When sampling, the first storage box 1 connected to the first connecting ring 3 is removed from the second connecting ring 4 for centralized collection and destruction. After collecting the sample, the test rod 7 removes the second connecting ring 4 from the second storage box 5, and the sampling head 8 at the front end of the test rod 7 is passed downward through the second tin foil 18 to immerse it in the salt water 19. The second tin foil 18 provided here can prevent the salt water 19 from leaking out before it is used, and also ensures that the salt water 19 is not invaded by external bacteria before it is used, which will affect the detection structure;

[0040] Step 3: To ensure the personal safety of medical staff, reduce contact with the test rod 7 and avoid improper operation to injure fingers when breaking the test rod 7 to reduce the chance of infection, press the thumb on the finger pressing groove 30 of the card 6, push the card 6 inward to make the slider 23 slide forward in the second slide groove 22 to make the first magnetic layer 24 and the second magnetic layer 25 adsorbed, and the first serration 26 at the front end of the card 6 and the second serration 29 of the third fixed edge 27 can cut off the test rod 7. Because the first magnetic layer 24 and the second magnetic layer 25 of the slider 23 of the card 6 adsorb each other, the second serration 29 and the first serration 26 can keep in a matching state after cutting, so as to prevent the saline 19 from leaking out, which plays a protective role for the sample and also prevents the virus from affecting the outside world.

[0041] Working principle: When in use, first, connect the first storage box 1 and the second storage box 5 together through the first connecting ring 3 and the second connecting ring 4 set between them, first remove the first storage box 1 through the third thread 14, and pull the sampling head 8 out of the first tin foil 16 through the test rod 7. The set first tin foil 16 can ensure the tight effect without affecting the removal during use. The sampling head 8 at the front end of the test rod 7 is used to sample the patient, thereby improving the convenience and efficiency of using the nasopharyngeal swab. When sampling, the first storage box 1 is connected to the first connecting ring 3, removed from the second connecting ring 4, and collected and destroyed. After collecting the sample, the test rod 7, the second connecting ring 4 is removed from the second storage box 5, and the sampling head 8 at the front end of the test rod 7 is passed downward through the second tin foil 18 to immerse it in saline 19. The second tin foil 18 set here can avoid salt before use. Water 19 leaks out, which also ensures that the salt water 19 is not invaded by external bacteria before use, and affects the detection structure. In order to ensure the personal safety of medical staff, reduce contact with the test rod 7 and avoid improper operation to injure fingers when breaking the test rod 7 to reduce the chance of infection, press the thumb on the finger pressing groove 30 of the card 6, push the card 6 inward to make the slider 23 slide forward in the second slide groove 22 to make the first iron-absorbing layer 24 and the second iron-absorbing layer 25 adsorb, and the first serration 26 at the front end of the card 6 and the second serration 29 of the third fixed edge 27 can cut off the test rod 7. Because the first iron-absorbing layer 24 and the second iron-absorbing layer 25 of the slider 23 of the card 6 adsorb each other, the second serration 29 and the first serration 26 can keep in a matching state after cutting, avoiding the leakage of salt water 19 to protect the sample and also avoiding the influence of viruses on the outside.

[0042] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A rapid extraction tool for nucleic acid in a nasopharyngeal swab sample, comprising a first storage box (1), characterized in that: The first storage box (1) is provided with an abrasive belt (2), the first storage box (1) is provided with a first connecting ring (3), the lower end of the first connecting ring (3) is provided with a second connecting ring (4), the second connecting ring (4) is provided with a second storage box (5), the second storage box (5) is provided with an insertion card (6), the first storage box (1) is provided with a test rod (7), the upper end of the test rod (7) is provided with a sampling head (8), the first connecting ring (3) is provided with a rubber layer (9), The rubber layer (9) is provided with an opening (10), the opening (10) is snap-connected with the upper end of the test rod (7), the first storage box (1) is provided with a vacuum box (15), the lower end of the vacuum box (15) is provided with a first tin foil (16), the vacuum box (15) is movably connected with the sampling head (8), the second storage box (5) is provided with a first fixed edge (17), the first fixed edge (17) is provided with a second tin foil (18), the lower end of the second tin foil (18) is provided with a salt water (19 ), a first slide groove (20) is provided in the second storage box (5), the first slide groove (20) is slidably connected to the plug-in card (6), a second fixed edge (21) is provided in the second storage box (5), the second fixed edge (21) is fixedly connected to the first slide groove (20), a symmetrical second slide groove (22) is provided in the second fixed edge (21), symmetrical sliders (23) are provided at the upper and lower ends of the plug-in card (6), and symmetrical first magnetic layers (22) are provided at the front ends of the two sliders (23). 4), symmetrical second magnetic absorption layers (25) are arranged in the two second slide grooves (22), the two second magnetic absorption layers (25) are movably connected to the first magnetic absorption layer (24), a third fixed edge (27) is arranged in the second storage box (5), the third fixed edge (27) corresponds to the plug-in card (6), a second groove (28) is provided at the front end of the third fixed edge (27), a second sawtooth (29) is provided in the second groove (28), and a finger pressing groove (30) is provided on one side of the plug-in card (6).

2. The rapid extraction tool for nucleic acid in nasopharyngeal swab samples according to claim 1, characterized in that: A first groove (11) is provided in the second connecting ring (4), a first thread (12) is provided at the lower end of the first connecting ring (3), the first thread (12) is movably connected to the first groove (11), a second thread (13) is provided in the second connecting ring (4), and a third thread (14) is provided in the first connecting ring (3).

3. The rapid extraction tool for nucleic acid in nasopharyngeal swab samples according to claim 2, characterized in that: The front end of the plug-in card (6) is provided with a first sawtooth (26), and the first sawtooth (26) is made of stainless steel.

4. The method for using the rapid extraction tool for nucleic acid in nasopharyngeal swab samples according to claim 3, characterized in that: The following steps are involved: Step 1: First, the first storage box (1) and the second storage box (5) are connected together by a first connecting ring (3) and a second connecting ring (4) arranged between them. The first storage box (1) is first removed by means of the third thread (14). The sampling head (8) is pulled out from the first tin foil (16) through the test rod (7). The first tin foil (16) is provided to ensure a tight seal without affecting the removal during use. The sampling head (8) at the front end of the test rod (7) is used to collect samples from the patient, thereby improving the convenience and efficiency of using the nasopharyngeal swab. Step 2: When sampling, the first storage box (1) is connected to the first connecting ring (3) and removed from the second connecting ring (4) for centralized collection and destruction. After collecting the sample, the test rod (7) removes the second connecting ring (4) from the second storage box (5), and the sampling head (8) at the front end of the test rod (7) is passed downward through the second tin foil (18) to immerse it in the salt water (19). The second tin foil (18) provided here prevents the salt water (19) from leaking out before use, and also ensures that the salt water (19) is not invaded by external bacteria before use, which may affect the detection structure; Step 3: To ensure the personal safety of medical personnel, reduce contact with the test rod (7) and avoid improper operation to injure fingers when breaking the test rod (7) to reduce the probability of infection, the thumb is pressed on the finger pressing groove (30) of the insertion card (6), and the insertion card (6) is pushed inward to make the slider (23) slide forward in the second slide groove (22) so that the first iron-absorbing layer (24) and the second iron-absorbing layer (25) are adsorbed. When the first sawtooth (26) at the front end of the insertion card (6) and the second sawtooth (29) of the third fixed edge (27) are engaged, the test rod (7) is cut off. Also, because the first iron-absorbing layer (24) and the second iron-absorbing layer (25) of the slider (23) of the insertion card (6) are mutually adsorbed, the second sawtooth (29) and the first sawtooth (26) remain in a matching state after cutting, thereby preventing the saline (19) from leaking out, which plays a protective role for the sample and also prevents the virus from affecting the outside world.

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