All-in-one kit

Abstract

The integrated test kit includes a storage area and a paving area; the paving area is used for holding and paving the sample mixture to be observed; the storage area is used for storing the sample mixture; the storage area and the paving area are connected by a connecting device; the integrated test kit also includes a liquid level balancing device; when the sample mixture is poured from the storage area to the paving area, the liquid level balancing device is used to discharge the gas in the paving area; after the sample mixture is paved, the liquid level balancing device is used to stabilize the liquid level in the paving area. With the liquid level balancing device, the sample mixture in the storage area can quickly and fully enter the paving area to complete the paving of the sample mixture, forming a stable liquid level state for observation.

Description

Technical Field

[0001] The present application belongs to the field of integrated reagent kit devices, and in particular to a reagent device integrating sample mixture storage and sample mixture tiling. Background Art

[0002] It is a common operation in sample inspection to make a sample mixture to be observed and spread it flat, and then observe it under a microscope. The sample mixture needs a flat field of view or observation area for microscopic observation, and the sample mixture needs to form a uniform and stable liquid surface to be observed.

[0003] In some existing reagent devices that integrate sample mixture storage and sample mixture flattening, a cover sheet is provided above the flattening area, and a vent is provided on the cover sheet. However, for liquid observation samples, the various components in them are originally very small, and the presence of the vent will cause the liquid or other components in the sample mixture to evaporate, thereby affecting the observability of the liquid surface and the accuracy of the observation results. Some may even dry up, making it impossible to observe the suspension.

[0004] In the existing reagent device that integrates the storage and tiling of sample mixture, the tiling area is sealed to solve the above problem. However, due to the limitation of the material and process of making the integrated reagent kit, although the tiling area is sealed, it is usually still in a non-vacuum state, that is, there is gas in the tiling area before it is filled with sample mixture.

[0005] When the sample mixture is perfused from the storage area into the paving area, if there is no corresponding connecting or dredging mechanism, the gases in the paving area will be compressed during the process of perfusing the sample mixture. These gases will counteract the progress of the perfusion, slowing down the perfusion process, and the residual gases will make it impossible for the sample mixture in the paving area to be stably spread, making the liquid surface of the spread sample mixture unstable and unable to be used for observation. Summary of the invention

[0006] The technical problem to be solved by the present application is to avoid the above-mentioned deficiencies of the prior art, and propose an integrated reagent kit with a liquid level balancing device, so that the sample mixture in the storage area can quickly and fully enter the tiling area to complete the tiling of the sample mixture, forming a stable liquid level state for observation.

[0007] The solution of the present invention to solve the above-mentioned technical problems is an integrated test kit, including a storage area and a spreading area; the spreading area is used to hold and spread the sample mixture to be observed; the storage area is used to store the sample mixture; the storage area and the spreading area are connected by a connecting device; the integrated test kit also includes a liquid level balancing device; when the sample mixture is poured from the storage area to the spreading area, the liquid level balancing device is used to discharge the gas in the spreading area; after the sample mixture is spread, the liquid level balancing device is used to stabilize the liquid level in the spreading area.

[0008] The connecting device comprises a first channel; one end of the first channel is connected to the storage area, and the other end of the first channel is connected to a side of the paving area close to the storage area.

[0009] The liquid level balancing device comprises a second channel; one end of the second channel is connected to the storage area, and the other end of the second channel is connected to a side of the paving area away from the storage area.

[0010] The liquid level balancing device also includes a second switch valve; the second switch valve is used to control the connection state of the two ends of the second channel; when the second switch valve is opened, the two ends of the second channel are connected; when the second switch valve is closed, the second channel is closed.

[0011] The liquid level balance device also includes a second power device; the second power device is used to extract the gas or sample mixed liquid in the flattening area from a side of the flattening area away from the storage area.

[0012] The second power device is arranged in the second channel, or the second power device is arranged outside the tiling area.

[0013] The liquid level balance device includes a third space; the third space is arranged on a side of the tiling area away from the storage area; one side of the third space is connected to the tiling area, and the other side of the third space is closed, or the other side of the third space is connected to the outside; the volume of the third space is greater than or equal to the volume of the tiling area for accommodating the sample mixture.

[0014] The liquid level balance device also includes a third switch valve; the third switch valve is used to control the connection state between the third space and the paving area; when the third switch valve is opened, the third space and the paving area are connected; when the third switch valve is closed, the third space and the paving area are not connected.

[0015] The liquid level balance device also includes a third power device; the third power device is used to extract the gas or sample mixed liquid in the paving area from a side of the paving area away from the storage area.

[0016] The third space is a third pipeline, and the third pipeline is a pipeline with a gradually changing inner diameter; the inner diameter of the third pipeline at the end connected to the paving area is greater than the inner diameter of the third pipeline at the end.

[0017] The minimum aperture of the third pipe is between 0.5mm and 0.01mm (millimeter)

[0018] The third pipeline is a communicating pipeline that is bent multiple times.

[0019] The third pipe is a horizontal capital letter U-shaped pipe or a capital letter S-shaped pipe.

[0020] The liquid level balancing device also includes a first switch valve; the first switch valve is used to control the connectivity status of the two ends of the first channel; when the first switch valve is opened, the two ends of the first channel are connected, that is, the paving area and the storage area are connected; when the first switch valve is closed, the first channel is closed, that is, the paving area and the storage area are not connected.

[0021] When the first switch valve is closed, the paving area is in a vacuum state, or the paving area is in a negative pressure state relative to the atmosphere.

[0022] The liquid level balance device also includes a first power device; the first power device is used to send the sample mixture in the storage area into the paving area.

[0023] The first power device includes any one or more of a micro pump, a peristaltic pump, an external air pressure source or a syringe.

[0024] The first power device includes a centrifugal device.

[0025] The aperture range of the first channel is 0.1 mm to 5 mm; the length range of the first channel is 2 mm to 500 mm.

[0026] The aperture range of the second channel is 0.1 mm to 5 mm; the length range of the second channel is 2 mm to 500 mm.

[0027] The aperture range of the connecting end of the third pipeline and the paving area is 0.1 mm to 5 mm; the length range of the third pipeline is 2 mm to 500 mm.

[0028] The second power device includes a negative pressure pump or a negative pressure propulsion device.

[0029] The third power device includes a negative pressure pump or a negative pressure propulsion device.

[0030] The storage area is characterized in that the bottom surface of the flattening area is higher than the bottom surface of the storage area, or the bottom surface of the flattening area is flush with the bottom surface of the storage area.

[0031] The storage area and the tiling area are arranged on the same reagent box platform; in the first state, the reagent box platform is placed vertically, and the tiling area is located above the storage area; the storage area is used to store the sample mixture, and the sample mixture in the storage area does not flow into the tiling area; in the second state, the reagent box platform is placed horizontally, and the tiling area and the storage area are on the same horizontal plane; the sample mixture in the storage area flows into the sinking space of the tiling area under the action of gravity.

[0032] When the reagent kit platform is placed horizontally, the longitudinal height of the second channel is higher than the longitudinal height of the first channel.

[0033] The height difference between the longitudinal height of the second channel and the longitudinal height of the first channel is greater than or equal to the depth of the sample mixture in the tiling area.

[0034] The tiling area includes a supporting body; a sinking space is provided on the supporting body, and the sinking space is connected to the storage area; an observation area is provided in the sinking space, and the observation area is used for tiling the sample mixture to be observed to form an observation field for microscopic examination; after the sample mixture enters the tiling area, it diffuses and covers the observation area, the sample mixture to be observed is tiled in the observation area, and the cells or particles in the sample mixture stay or suspend in the observation area for observation or imaging.

[0035] A cover sheet is arranged on the observation area; the cover sheet is embedded above the sinking space, and a cover sheet support structure is arranged on the four edges of the sinking space to support the cover sheet; the sinking space covered by the cover sheet forms a space for accommodating the sample mixture.

[0036] Compared with the prior art, the beneficial effects of the present application are: 1. The design of the connecting device and the liquid level balance device between the storage area and the tiling area can prevent bubbles from gathering in the closed or non-closed tiling area, so that the sample mixture can quickly flow to the tiling area with the help of the air pressure balance or external force balance between the storage area and the tiling area, and discharge the original gas in the tiling area; whether it is a tiling area connected to the external atmosphere or a completely sealed tiling area, the technical solution in the present application can make the sample mixture quickly and fully tiling; 2. The power device arranged in the liquid level balance device further speeds up the tiling speed, so that the sample mixture to be observed can quickly complete the tiling process, which is beneficial to subsequent observation. and imaging; 3. The shut-off structure, such as a valve, provided in the liquid level balance device can increase the spatial independence between the storage area and the tiling area, so that the liquid surface after tiling can quickly and naturally enter a stable state, avoiding the disturbance of the liquid or gas in the storage area or other factors on the liquid surface in the tiling area; 4. The multiple-bend pipeline design and the gradient pipe diameter pipeline design, on the one hand, avoid the direct contact of the sample mixture in the tiling area with the outside, causing unnecessary evaporation or volatilization, affecting the observation; and provide a channel for the gas in the tiling area before infusion to come out; at the same time, it also provides space for caching the sample mixture in the pipeline, and the sample mixture cached in the pipeline plays the role of closing the tiling area. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is one of the axonometric projection schematic diagrams of one of the preferred embodiments of the integrated kit;

[0038] Figure 2 It is a second axonometric projection schematic diagram of one of the preferred embodiments of the integrated reagent kit;

[0039] Figure 3 It is a front view orthographic projection schematic diagram of one preferred embodiment of the integrated kit;

[0040] Figure 4 yes Figure 3 DD cross-sectional diagram;

[0041] Figure 5 yes Figure 4 FF cross-sectional diagram;

[0042] Figure 6 It is one of the axonometric projection diagrams of the second preferred embodiment of the integrated reagent kit, Figure 6 The integrated test kit is in a vertical upright position;

[0043] Figure 7 is a second axonometric projection diagram of the second preferred embodiment of the integrated reagent kit, Figure 7 The integrated reagent kit is in a horizontally flat state; in this state, the sample mixture in the storage area will flow to the flattening area;

[0044] Figure 8 It is a top view orthographic projection schematic diagram of the third preferred embodiment of the integrated reagent kit;

[0045] Fig. 9 It is a top view orthographic projection schematic diagram of the fourth preferred embodiment of the integrated reagent kit;

[0046] Fig.10 It is a top view orthographic projection schematic diagram of the fifth preferred embodiment of the integrated reagent kit;

[0047] Fig.11 It is a top view orthographic projection schematic diagram of the sixth preferred embodiment of the integrated reagent kit;

[0048] Fig.12 It is a top view orthographic projection schematic diagram of the seventh preferred embodiment of the integrated reagent kit. DETAILED DESCRIPTION

[0049] The implementation methods of the present application are further described in detail below in conjunction with the accompanying drawings.

[0050] like Figures 1 to 7 In the embodiment of the integrated test kit shown, it includes a storage area 100 and a spreading area 200; the spreading area 200 is used for storing and spreading the sample mixture to be observed in microscopy; the storage area 100 is used for storing the sample mixture; the storage area 100 and the spreading area 200 are connected by a connecting device; the integrated test kit also includes a liquid level balancing device; when the sample mixture is poured from the storage area to the spreading area, the liquid level balancing device is used to discharge the gas in the spreading area; after the sample mixture is spread, the liquid level balancing device is used to stabilize the liquid level in the spreading area.

[0051] The liquid level balance device is set up so that the sample mixture in the storage area 100 can quickly and fully enter the splaying area to complete the splaying of the sample mixture. Quickly entering the splaying area means that there is no pause or obstruction in the process of the sample mixture entering, and the splaying of the sample mixture in the splaying area is completed within 10 seconds to 120 seconds. Fully entering means that the splaying area is filled with the sample mixture without bubbles and can be used for microscopic observation or imaging; the sample mixture in the splaying area is splaying in place without disturbance.

[0052] Due to the sealing process, the tiling area needs to be made into a vacuum-sealed state, which has very high requirements for the process and is also very costly. Therefore, there is usually air in the tiling area. Even if the tiling area is in a negative pressure state relative to the atmospheric pressure, there will still be some gas in the tiling area. When the sample mixture enters it, the bubbles of residual gas need to be excluded from the observation area. If bubbles exist, on the one hand, it will affect the tiling of the sample mixture in the observation area, and on the other hand, it will also increase the disturbance or uncertainty of the tiling area, because bubbles may cause disturbances in the sample mixture in the observation area when they are slightly disturbed or when the state changes due to temperature or other reasons, thereby affecting the observation or imaging process.

[0053] like Figures 1 to 7 In the embodiment of the integrated reagent kit shown, the connecting device includes a first channel 700; one end of the first channel 700 is connected to the storage area 100, and the other end of the first channel 700 is connected to a side of the tiling area 200 close to the storage area.

[0054] like Figures 1 to 7 In the embodiment of the integrated reagent kit shown, the liquid level balance device includes a second channel 800; one end of the second channel 800 is connected to the storage area 100, and the other end of the second channel 800 is connected to the side of the tiling area 200 away from the storage area.

[0055] like Figures 1 to 7 In the embodiment of the integrated test kit shown, the first channel 700 and the second channel 800 are both connected to the storage area 100, and the other end of the second channel 800 is connected to the side of the tiling area 200 away from the storage area; when the sample mixture enters the tiling area 200, the gas in the tiling area 200 can be discharged through the second channel 800, so that the sample mixture can quickly enter the tiling area to complete the tiling process. After the tiling is completed, the first channel 700 and the second channel 800 are both connected to the storage area 100, so that the pressure balance can be maintained between the inlet and outlet of the sample mixture in the tiling area, so that the liquid level in the observation area is stable and undisturbed.

[0056] In some embodiments of the integrated reagent kit not shown in the accompanying drawings, the storage area is characterized in that the bottom surface of the tiling area is higher than the bottom surface of the storage area, or the bottom surface of the tiling area is flush with the bottom surface of the storage area. In this way, when the sample mixture is stored in the storage area, the liquid surface of the sample mixture has a height difference relative to the bottom surface of the tiling area. When the sample mixture can flow into the tiling area with the help of gravity or the potential energy stored in the storage area, such energy or force can smoothly and quickly fill the tiling area, so that the gas in the tiling area is quickly discharged, and no bubbles are generated in the tiling area to counterbalance and affect subsequent observation or imaging.

[0057] like Figures 1 to 7In the embodiment of the integrated reagent kit shown, the storage area 100 and the tiling area 200 are arranged on the same reagent kit platform 900. Figure 6 As shown, in the first state, the reagent kit platform 900 is placed vertically, and the tiling area 200 is located above the storage area 100; the storage area 100 is used to store the sample mixture, and the sample mixture in the storage area 100 does not flow into the tiling area. Figure 7 As shown, in the second state, the reagent kit platform 900 is placed horizontally, and the bottom surfaces of the tiling area 200 and the storage area 100 are on the same horizontal plane; the overall height of the storage area 100 is higher than the tiling area 200; when the sample mixture is stored in the storage area 100, the liquid level of the sample mixture is higher than the bottom surface of the tiling area 200, forming a potential difference. After the reagent kit platform 900 is placed horizontally, the sample mixture in the storage area 100 flows into the tiling area 200 under the action of gravity.

[0058] like Figures 1 to 7 In the embodiment of the integrated reagent kit shown, the tiling area 200 includes a carrier body 210; a sinking space 230 is provided on the carrier body 210, and the sinking space 230 is connected to the storage area 100; an observation area 260 is provided in the sinking space 230, and the observation area 260 is used for tiling the sample mixture to be observed to form an observation field for microscopic examination; after the sample mixture enters the tiling area, it diffuses and covers the observation area 260, and the sample mixture to be observed is tiled in the observation area 260, and the cells or particles in the sample mixture stay or suspend in the observation area 260 for observation or imaging. After entering the tiling area 200, the sample mixture to be observed needs a stable and undisturbed environment so that a stable image can be obtained during observation or imaging.

[0059] like Figures 1 to 7 In the embodiment of the integrated reagent kit shown, a cover sheet 290 is provided on the observation area 260; the cover sheet 290 is embedded above the sinking space 230, and the sinking space covered by the cover sheet forms a space for accommodating the sample mixture. The four edges of the sinking space 230 are provided with a cover sheet support structure 240; an overflow space 231 is also provided between the observation area 260 and the cover sheet support structure 240; when the integrated reagent kit is placed horizontally, the longitudinal height of the position where the top surface of the observation area 260 is located is less than or equal to the longitudinal height of the position where the top surface of the cover sheet support structure 240 is located.

[0060] like Figure 1 and Figure 2In the embodiment of the integrated reagent kit shown, the observation area 260 is a space with a square cross section; a cover sheet 290 is provided on the observation area 260; the shape and size of the cover sheet correspond to the shape and size of the observation area, and can be embedded and covered on the observation area. The cover sheet 290 is embedded above the sinking space 230, and the cover sheet support structure 240 supports the cover sheet 290, so that the sinking space 230 covered by the cover sheet 290 forms a sealed space for containing and paving the sample mixture to be observed; when the cover sheet 290 is covered on the cover sheet support structure 240, the space height between the top surface of the observation area 260 and the bottom surface of the cover sheet 290 ranges from 0.05mm to 0.35mm, which is just suitable for paving the suspended particles in the sample mixture.

[0061] In some embodiments not shown in the drawings, the cover sheet 290 is square, and the side length of the square cover sheet 290 ranges from 6 mm to 34 mm. The thickness of the cover sheet 290 ranges from 0.08 mm to 0.3 mm, or the thickness of the cover sheet is 0.17 mm; the thickness of the cover sheet is less than or equal to the sinking depth of the cover sheet support structure 240 relative to the top surface of the carrier body 210.

[0062] In some embodiments not shown in the drawings, the thickness range of the carrier body 210 is 0.8 mm to 2.0 mm, and the thickness of the carrier body is 1.1 mm; the length range of the carrier body is 15 to 100 mm, or the length of the carrier body is 50 mm; the width range of the carrier body is 10 to 50 mm, or the width of the carrier body is 30 mm.

[0063] In some embodiments not shown in the drawings, the tiling area is connected to the storage area; when the integrated reagent box is placed horizontally, the bottom surface of the tiling area can be flush with the bottom surface of the storage area, or the bottom surface of the tiling area can be lower than the bottom surface of the storage area. Regardless of the relative heights of the bottom surfaces of the storage area and the sample processing area, when the integrated reagent box is switched from a vertical placement to a horizontal placement, the liquid level of the sample mixture in the storage area is higher than the bottom surface of the tiling area, so that the sample mixture can enter the tiling area from the storage area under the action of gravity.

[0064] In some embodiments not shown in the drawings, when the reagent kit platform is placed horizontally, the longitudinal height of the second channel is higher than the longitudinal height of the first channel. That is to say, when the integrated reagent kit is placed horizontally, the second channel and the first channel can be on the same longitudinal section, and the horizontal position of the second channel should not be lower than the horizontal position of the first channel. Of course, the second channel and the first channel can also be on different longitudinal sections, and the horizontal position of the second channel should be higher than the horizontal position of the first channel, or the horizontal position of the second channel is flush with the horizontal position of the first channel, to ensure that the gas in the paving area can be discharged through the second channel.

[0065] In some embodiments not shown in the drawings, when the reagent cartridge platform is placed horizontally, the height difference between the longitudinal height of the second channel and the longitudinal height of the first channel is greater than or equal to the depth of the sample mixture in the tiling area.

[0066] like Figures 1 to 7 In the embodiment of the integrated reagent kit shown, reference numeral 130 is a storage subspace of the storage area 100 for storing the sample mixture to be plated.

[0067] like Figure 3 and Figure 4 As shown, the second channel 800 is used for the liquid in the tiling area 200 to flow back to the storage area 100; the sample mixture passes through the second channel 800 and re-enters the storage area, and both ends of the tiling area are connected to the storage area to form a communicating vessel structure and achieve pressure balance at both ends. At this time, there is no lateral flow of the sample mixture in the tiling area.

[0068] like Figure 3 and Figure 4 As shown, the first connecting hole 133 is the connecting hole between the first channel 700 and the storage area 100; the second connecting hole 136 is the location of the connection between the second channel 800 and the storage area 100. The first connecting hole 133 and the second connecting hole 136 are arranged on the side wall of the storage subspace 130. The first connecting hole 133 and the second connecting hole 136 can also be arranged on the side wall of the storage area 100 connected to the tiling area. When the integrated reagent kit is placed horizontally, the first connecting hole 133 is close to the bottom 135 of the storage area, and the second connecting hole 136 is farther from the bottom 135 of the storage area. The distance between the second connecting hole 136 and the first connecting hole 133 is greater than or equal to the sinking depth of the sinking space of the tiling area.

[0069] In some embodiments not shown in the drawings, the aperture range of the first channel 700 may be larger than the aperture range of the second channel 800. The first channel 700 has a large aperture and a small fluid resistance. The second channel 800 has a small aperture and a large fluid resistance. This facilitates the sample mixture to flow into the tiling area. The sample mixture will form a hydraulic pressure at one end of the second channel, which is used to counteract the gas pressure at the end of the second channel when the second channel is closed, so that the liquid level in the tiling area is stabilized. The sample mixture will enter the second channel, and when the end of the second channel is opened, the liquid entering this section is connected to the outside, so that the liquid level in the tiling area is stabilized; at the same time, the liquid entering this section also plays a role in closing the tiling area.

[0070] like Figures 8 to 12 In the embodiment of the integrated reagent kit shown, reference numeral 130 is a subspace of the storage area 100, which is used to store the sample mixture to be plated. Reference numeral 260 is an observation area, and reference numeral 231 is an overflow area, i.e., an overflow space.

[0071] like Figure 8In the embodiment of the integrated test kit shown, the liquid level balancing device also includes a second switch valve 910; the second switch valve 910 is used to control the connectivity between the two ends of the second channel 800; when the second switch valve 910 is opened, the two ends of the second channel 800 are connected; when the second switch valve 910 is closed, the second channel 800 is closed.

[0072] like Fig. 9 In the embodiment of the integrated kit shown, the liquid level balancing device further comprises a second power device; Fig. 9 The second power device is a push-pull rod device 930; the second power device is used to extract the gas or sample mixture in the paving area from the side of the paving area away from the storage area. The second power device can be arranged in the second channel 800, or the second power device can also be arranged outside the paving area. The second power device includes a negative pressure pump or a negative pressure push device.

[0073] like Figures 10 to 12 In the embodiment of the integrated test kit shown, the liquid level balance device includes a third space 600; the third space 600 is arranged on a side of the tiling area away from the storage area; one side of the third space 600 is connected to the tiling area, and the other side of the third space 600 is closed, or the other side of the third space 600 is connected to the outside; the volume of the third space 600 is greater than or equal to the volume of the tiling area for accommodating the sample mixture.

[0074] The third space can be a tangible space, such as a square or circular space with gas or liquid accommodating capacity; the third space can also be a channel, which also forms a buffer space. When the sample mixture enters the flattening area, the gas in the flattening area enters the third space buffer.

[0075] In some embodiments of the integrated test kit not shown in the accompanying drawings, the liquid level balance device also includes a third switch valve; the third switch valve is used to control the state of connectivity between the third space and the tiling area; when the third switch valve is opened, the third space and the tiling area are connected; when the third switch valve is closed, the third space and the tiling area are not connected.

[0076] like Fig.10 In the embodiment of the integrated reagent kit shown, the liquid level balance device further includes a third power device 960; the third power device is used to extract the gas or sample mixture in the paving area from the side of the paving area away from the storage area. The third power device is arranged in the third space, or arranged outside the paving area. The third power device includes a negative pressure pump or a negative pressure driving device.

[0077] like Fig.12In the embodiment of the integrated reagent kit shown, the third space 600 is a third pipe, and the pipe of the third pipe is a pipe with a gradually changing inner diameter; the inner diameter of the third pipe at the end connected to the flat area is greater than the inner diameter of the third pipe at the end. A vent hole 680 is also provided at the end of the third pipe. In some embodiments not shown in the drawings, there may be no vent hole 680. The third space 600 is a connecting pipe with multiple bends.

[0078] like Figures 10 to 12 In the embodiment of the integrated kit shown, the third channel is a horizontal capital letter U-shaped channel or a capital letter S-shaped channel.

[0079] like Figures 10 to 12 In the embodiment of the integrated reagent kit shown, the third space is a third pipe, and the pipe of the third pipe is a pipe with a gradually changing inner diameter; the inner diameter of the third pipe at the end connected to the flat area is larger than the inner diameter of the third pipe at the end. The third pipe is designed with a gradually decreasing aperture, and the minimum aperture of the third pipe is between 0.5mm and 0.01mm, ensuring that the gas can be connected, but the liquid will not overflow due to the existence of molecular tension and large flow resistance.

[0080] In some embodiments of the integrated test kit not shown in the accompanying drawings, the liquid level balance device also includes a first switch valve; the first switch valve is used to control the connectivity status of the two ends of the first channel; when the first switch valve is opened, the two ends of the first channel are connected, that is, the tiling area and the storage area are connected; when the first switch valve is closed, the first channel is closed, that is, the tiling area and the storage area are not connected.

[0081] In some embodiments of the integrated reagent kit not shown in the drawings, when the first switch valve is closed, the tiling area is in a vacuum state, or the tiling area is in a negative pressure state relative to the atmosphere.

[0082] like Figures 10 to 12 In the embodiment of the integrated reagent kit shown, the liquid level balance device further includes a first power device; the first power device is used to deliver the sample mixture in the storage area to the flattening area. The first power device is arranged in the storage area, or the first power device is arranged in the first channel; or the first power device is arranged outside the storage area. The first power device includes any one or more of a micro pump, a peristaltic pump, an external air pressure source, or a syringe. Fig.11 The reference numeral 760 is a first power device, which is a micro pump.

[0083] In some embodiments of the integrated reagent kit not shown in the accompanying drawings, the first power device includes a centrifugal device. The centrifugal device acts on the integrated reagent kit as a whole, or the centrifugal device acts on the storage area, so that the sample mixture in the storage area enters the flattening area through the connecting device under the action of centrifugal force. A centrifugal force pointing to the flattening area can be applied to the integrated reagent kit manually, such as by shaking, or mechanically, such as by a centrifugal device, so that the sample mixture is driven by the centrifugal force to enter and fill the observation area in the flattening area through the flow channel, and when the centrifugal force disappears, the pressure at the inlet and outlet of the sample observation chamber is balanced, and there is no lateral flow of sample liquid.

[0084] In some embodiments of the integrated reagent kit not shown in the drawings, the aperture of the first channel ranges from 0.1 mm to 5 mm; the length of the first channel ranges from 2 mm to 500 mm. The aperture of the second channel ranges from 0.1 mm to 5 mm; the length of the second channel ranges from 2 mm to 500 mm. The aperture of the third conduit and the paving area connection end ranges from 0.1 mm to 5 mm; the length of the third conduit ranges from 2 mm to 500 mm.

[0085] The valve structure in the above embodiment includes a first switch valve, a second switch valve and a third switch valve, which are locked at the front end or the rear end or both of the front and rear ends of the sample spreading area to realize the control of the connectivity state between the sample storage area and the sample spreading area. The connectivity state between the sample storage area and the sample spreading area, combined with the respective states of the sample storage area and the sample spreading area, balances the pressure at the inlet and outlet of the sample spreading area and does not generate lateral flow.

[0086] In some embodiments not shown in the accompanying drawings, the first switch valve can be arranged on the first channel; when the storage area is storing the sample mixture or preparing the sample mixture, the first switch valve is locked; when the integrated reagent kit is in a flat state, the first switch valve is opened, and the sample mixture flows due to the hydraulic pressure difference under the action of gravity, passes through the first channel through the first switch valve, and enters the tiling area, and the sample mixture fills the observation area and overflows through the second channel; the first switch valve is locked, the connection between the storage area and the tiling area is disconnected, the inlet and outlet pressures of the tiling area are balanced, and there is no lateral flow.

[0087] like Figure 8 In the illustrated embodiment, the second switch valve is disposed on the second channel to control the communication state between the second channel and the tiling area.

[0088] In some embodiments not shown in the accompanying drawings, a first switch valve can be arranged on the first channel to control the connectivity between the storage area and the tiling area at both ends of the first channel; at the same time, a second switch valve is arranged on the second channel to control the connectivity between the second channel and the tiling area.

[0089] In the present application, the cells in the sample to be stained complete the staining process in a liquid suspension, and the environment used for the staining solution and the diluent is relatively close to the biological physiological state. Therefore, during the staining process, the cell activity is better maintained. On the one hand, the Brownian motion of the cells and the dye molecules in the solution, and the electrostatic force can be utilized to carry out the staining reaction, which makes the staining speed faster and more efficient.

[0090] The first power device in the above embodiment can be a micro pump, a peristaltic pump, an external air pressure source, a syringe, etc. The first power device can apply pressure to the sample mixture stored in the storage area, thereby increasing the pressure of the sample mixture in the storage area, pushing the sample mixture to flow to the flattening area and fill the observation area, and when the observation area or the flattening area is completely filled, stop applying pressure, so that the inlet and outlet pressures of the flattening area are balanced without lateral liquid flow.

[0091] The second power device and the third power device in the above embodiment can apply suction force to the liquid or gas in the tiling area so that negative pressure is generated at the outlet of the tiling area away from the storage area. The sample mixture flows from the storage area into the tiling area and fills the observation area. The pressure at the inlet and outlet of the tiling area is balanced and there is no lateral flow of liquid.

[0092] The above descriptions are merely embodiments of the present application and are not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. An integrated kit, characterized in that: Includes storage and tiling areas; A spreading area, used for holding and spreading the sample mixture to be observed; A storage area for storing sample mixtures; The storage area and the paving area are connected via a connecting device; The integrated kit also includes a liquid level balance device; When the sample mixed liquid is poured from the storage area to the flattening area, the liquid level balance device is used to discharge the gas in the flattening area; After the sample mixture is spread, the liquid level balance device is used to stabilize the liquid level in the spreading area; The communication device comprises a first channel; one end of the first channel is connected to the storage area, and the other end of the first channel is connected to a side of the paving area close to the storage area; The liquid level balancing device comprises a second channel; one end of the second channel is connected to the storage area, and the other end of the second channel is connected to a side of the paving area away from the storage area.

2. The integrated kit according to claim 1, characterized in that: The liquid level balancing device also includes a second switch valve; The second switch valve is used to control the connection state between the two ends of the second channel; When the second switch valve is opened, both ends of the second channel are connected; When the second switch valve is closed, the second channel is closed.

3. The integrated kit according to claim 1, characterized in that: The liquid level balancing device also includes a second power device; The second power device is used to extract the gas or sample mixed liquid in the flattening area from a side of the flattening area away from the storage area; The second power device is arranged in the second channel, or the second power device is arranged outside the tiling area.

4. The integrated kit according to claim 1, characterized in that: The liquid level balancing device includes a third space; The third space is arranged on a side of the paving area away from the storage area; One side of the third space is connected to the tiled area, and the other side of the third space is closed, or the other side of the third space is connected to the outside; The volume of the third space is greater than or equal to the volume of the paving area for accommodating the sample mixture.

5. The integrated kit according to claim 4, characterized in that: The liquid level balancing device further includes a third switch valve; The third switch valve is used to control the state of the third space being connected to the tiled area; When the third switch valve is opened, the third space and the tiling areas are connected; When the third switch valve is closed, the third space and the tiling area are not connected.

6. The integrated kit according to claim 4, characterized in that: The liquid level balancing device also includes a third power device; The third power device is used to extract the gas or sample mixed liquid in the flattening area from the side of the flattening area away from the storage area.

7. The integrated kit according to claim 4, characterized in that: The third space is a third pipeline, and the pipeline of the third pipeline is a pipeline with a gradually changing inner diameter; The inner diameter of the third pipe at the end communicating with the tiling area is greater than the inner diameter of the third pipe at the end.

8. The integrated kit according to claim 7, characterized in that: The minimum aperture of the third pipe is between 0.5 mm and 0.01 mm.

9. The integrated kit according to claim 7, characterized in that: The third pipeline is a communicating pipeline that is bent multiple times.

10. The integrated kit according to claim 9, characterized in that: The third pipe is a horizontal capital letter U-shaped pipe or a capital letter S-shaped pipe.

11. The integrated kit according to claim 1, characterized in that: The liquid level balancing device further comprises a first switch valve; The first switch valve is used to control the connection state between the two ends of the first channel; When the first switch valve is opened, the two ends of the first channel are connected, that is, the paving area and the storage area are connected; When the first switch valve is closed, the first channel is closed, that is, the paving area and the storage area are not connected.

12. The integrated kit according to claim 11, characterized in that: When the first switch valve is closed, the paving area is in a vacuum state, or the paving area is in a negative pressure state relative to the atmosphere.

13. The integrated kit according to claim 1, characterized in that: The liquid level balancing device also includes a first power device; The first power device is used to deliver the sample mixture in the storage area to the flattening area.

14. The integrated kit according to claim 13, characterized in that: The first power device includes any one or more of a micro pump, a peristaltic pump, an external air pressure source or a syringe.

15. The integrated kit according to claim 13, characterized in that: The first power device includes a centrifugal device.

16. The integrated kit according to claim 1, characterized in that: The aperture range of the first channel is 0.1 mm to 5 mm; The length of the first channel ranges from 2 mm to 500 mm.

17. The integrated kit according to claim 1, characterized in that: The aperture range of the second channel is 0.1 mm to 5 mm; The length of the second channel ranges from 2 mm to 500 mm.

18. The integrated kit according to claim 7, characterized in that: The aperture range of the third pipeline and the connecting end of the paving area is 0.1 mm to 5 mm; The length of the third pipe ranges from 2 mm to 500 mm.

19. The integrated kit according to claim 3, characterized in that: The second power device includes a negative pressure pump or a negative pressure propulsion device.

20. The integrated kit according to claim 6, characterized in that: The third power device includes a negative pressure pump or a negative pressure propulsion device.

21. The integrated kit according to claim 1, characterized in that: The storage area is characterized in that the bottom surface of the flattening area is higher than the bottom surface of the storage area, or the bottom surface of the flattening area is flush with the bottom surface of the storage area.

22. The integrated kit according to claim 1, characterized in that: The storage area and the tiling area are arranged on the same reagent box platform; In the first state, the reagent kit platform is placed vertically, and the tiling area is located above the storage area; the storage area is used to store the sample mixture, and the sample mixture in the storage area does not flow into the tiling area; In the second state, the reagent kit platform is placed horizontally, and the paving area and the storage area are on the same horizontal plane; the sample mixture in the storage area flows into the sinking space of the paving area under the action of gravity.

23. The integrated kit according to claim 22, characterized in that: When the reagent kit platform is placed horizontally, the longitudinal height of the second channel is higher than the longitudinal height of the first channel.

24. The integrated kit according to claim 23, characterized in that: The height difference between the longitudinal height of the second channel and the longitudinal height of the first channel is greater than or equal to the depth of the sample mixture in the tiling area.

25. The integrated kit according to claim 1, characterized in that: The paving area includes a bearing body; a sinking space is provided on the bearing body, and the sinking space is connected to the storage area; An observation area is provided in the sinking space, and the observation area is used for tiling the sample mixture to be observed to form an observation field of view for microscopic examination; After the sample mixture enters the flattening area, it diffuses and covers the observation area. The sample mixture to be observed is flattened in the observation area, and the cells or particles in the sample mixture stay or suspend in the observation area for observation or imaging.

26. The integrated kit according to claim 25, characterized in that A cover sheet is provided on the observation area; The cover sheet is embedded above the sinking space, and at the same time, a cover sheet support structure is arranged on the four edges of the sinking space to support the cover sheet; the sinking space covered by the cover sheet forms a space for accommodating the sample mixture.

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