Dual-optical path micro-droplet chip analyzer and control method thereof

Abstract

The present invention provides a dual-light path micro-droplet chip analyzer and a control method thereof, wherein the dual-light path micro-droplet chip analyzer comprises a frame having an operating platform, the operating platform is provided with a chip holder capable of placing a micro-droplet chip, the operating platform is also provided with a first detection light path component and a second detection light path component, the micro-droplet chip has at least two spaced channels, the first detection light path component can detect one of the two channels, and the second detection light path component can detect the other of the two channels. The present invention can realize synchronous detection of multiple samples, shorten detection time, and improve detection speed and efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of digital PCR analyzers, and in particular relates to a dual-light path micro-droplet chip analyzer and a control method thereof. Background Art

[0002] Digital PCR is the latest quantitative technology. It is based on the single-molecule PCR method to count nucleic acid quantification, which is an absolute quantitative method. It mainly uses microfluidics or droplet methods to disperse a large amount of diluted nucleic acid solution into the microreactor or droplets of the chip, and the number of nucleic acid templates in each reactor is less than or equal to 1. In this way, after the PCR cycle, the microdroplets are irradiated with light of a specific wavelength. The reactor with a nucleic acid molecule template will give a specific fluorescent signal, and the reactor without a template will not have a specific fluorescent signal. According to the relative proportion and the volume of the reactor, the nucleic acid concentration of the original solution can be calculated. With the development of science and technology and the needs of social life, medical institutions and scientific research institutes have put forward higher requirements on the working rhythm, detection speed, efficiency and throughput of microdroplet chip analyzers. Summary of the invention

[0003] Therefore, the technical problem to be solved by the present invention is to provide a dual-optical path micro-droplet chip analyzer and a control method thereof, which can realize synchronous detection of multiple samples, shorten the detection time, and improve the detection speed and efficiency.

[0004] In order to solve the above problems, the present invention provides a dual-optical path micro-droplet chip analyzer, including a frame with an operating platform, on which a chip holder capable of placing a micro-droplet chip is arranged, and a first detection optical path component and a second detection optical path component are also arranged on the operating platform. The micro-droplet chip has at least two spaced-apart channels, and the first detection optical path component can detect one of the two channels, while the second detection optical path component can detect the other of the two channels.

[0005] Preferably, the first detection optical path component comprises an optical path component and a position adjustment component, wherein the position adjustment component can adjust the horizontal position and vertical position of the optical path component.

[0006] Preferably, the position adjustment component includes an adjustment positioning block and a support plate, the support plate is fixed to the operating platform through the adjustment positioning block, an X-axis position adjustment component is connected to the support plate, the X-axis position adjustment component is connected to the Y-axis position adjustment component, the Y-axis position adjustment component is connected to the optical path component, wherein the X-axis position adjustment component can adjust the horizontal position of the Y-axis position adjustment component and the optical path component, and the Y-axis position adjustment component can adjust the vertical position of the optical path component.

[0007] Preferably, the micro-droplet chip includes at least four channels, and the at least four channels are evenly spaced. The chip seat can be translated in the horizontal direction. The first detection optical path component and the second detection optical path component are spaced along the translation path of the chip seat, and the spacing between the first detection optical path component and the second detection optical path component is at least twice the spacing between two adjacent channels.

[0008] Preferably, a chip motion guide rail is fixedly connected to the operating platform, and the chip seat is slidably connected to the chip motion guide rail via a chip motion slider.

[0009] Preferably, a crimping assembly is also connected to the operating platform, and a crimping plate is also connected to the operating platform at a position corresponding to the crimping assembly. The chip holder has a crimping detection position in a first space formed between the crimping plate and the crimping assembly, and a chip loading and unloading position outside the first space. When the chip holder is in the crimping detection position, the crimping plate and the crimping assembly are respectively located on the upper and lower sides of the chip holder.

[0010] Preferably, the crimping assembly includes a guide rail fixing plate, a vertical screw motor is fixedly connected to the guide rail fixing plate via a vertical motor fixing plate, the vertical screw motor is connected to an oil injection needle via a vertical motor screw connector, the oil injection needle and the guide rail fixing plate are slidably connected via a crimping sliding assembly, and the guide rail fixing plate is fixedly connected to the operating platform.

[0011] The present invention also provides a control method for a dual-light-path micro-droplet chip analyzer, which is used to control the dual-light-path micro-droplet chip analyzer, comprising:

[0012] Injecting the micro-droplet chip into the chip holder at the chip loading and unloading position;

[0013] Controlling the chip horizontal driving assembly to operate, the chip holder carries the micro-droplet chip and moves it from the chip loading position to the crimping detection position, and when the micro-droplet chip is at the crimping detection position, the first detection optical path assembly and the second detection optical path assembly are respectively opposite to two non-adjacent channels on the micro-droplet chip;

[0014] Controlling the operation of the crimping assembly to lift the micro-droplet chip to contact the lower surface of the crimping plate, and controlling the oil injection needle in the crimping assembly to crimp the oil interface of the micro-droplet chip;

[0015] Controlling the first detection optical path component and the second detection optical path component to be turned on at the same time, and making the objective lenses of the first detection optical path component and the second detection optical path component respectively focus on the corresponding channels;

[0016] The oil is controlled to enter the oil injection needle and then enter the corresponding channel of the micro-droplet chip to complete the detection of the samples in the two non-adjacent channels.

[0017] Preferably, after completing the detection of the samples in the two non-adjacent channels, the control method further comprises:

[0018] Controlling the first detection optical path component and the second detection optical path component to be closed, and controlling the crimping component to reversely operate and return to zero;

[0019] Controlling the horizontal driving assembly to operate again to move the chip holder further along the direction from the chip loading position to the crimping detection position by a displacement of one channel pitch;

[0020] Controlling the operation of the crimping assembly to lift the micro-droplet chip to contact the lower surface of the crimping plate, and controlling the oil injection needle in the crimping assembly to crimp the oil interface of the micro-droplet chip;

[0021] Controlling the first detection optical path component and the second detection optical path component to be turned on at the same time, and making the objective lenses of the first detection optical path component and the second detection optical path component respectively focus on the corresponding channels;

[0022] The oil is controlled to enter the oil injection needle and then into the corresponding channel of the micro-droplet chip to complete the detection of the sample in the channel.

[0023] Preferably, the control method further includes:

[0024] After the samples in all channels in the micro-droplet chip are tested, the horizontal driving assembly is controlled to operate in reverse to move the chip holder from the crimping test position to the chip loading position, and the micro-droplet chip is unloaded at the chip loading position.

[0025] The present invention provides a dual-optical path micro-droplet chip analyzer and a control method thereof, which simultaneously uses the first detection optical path component and the second detection optical path component to perform synchronous detection of multiple samples on two different channels on the micro-droplet chip without interfering with each other, and can analyze the received signals separately, which significantly reduces the chip detection time and improves work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram (disassembled) of a dual-light-path micro-droplet chip analyzer according to an embodiment of the present invention;

[0027] Figure 2 for Figure 1 A schematic diagram of the structure of the first detection optical path component (disassembled);

[0028] Figure 3 for Figure 2 Schematic diagram of the structure of the position adjustment component (disassembled);

[0029] Figure 4 for Figure 2 Schematic diagram of the light transmission principle of the optical path component in FIG. 1 (the arrows in the figure show the light transmission path);

[0030] Figure 5 for Figure 1 Schematic diagram of the structure of the crimping assembly (disassembled).

[0031] The reference numerals are:

[0032] 100, first detection optical path component; 110, optical path component; 120, position adjustment component; 200, second detection optical path component; 300, crimping component; 1, operating platform; 2, chip motion guide rail; 3, chip motion slider; 4, chip seat; 5, micro droplet chip; 6, crimping plate; 7, horizontal screw motor; 8, nut connector; 9, support column; 10, adjustment positioning block; 11, support plate; 12, optical path connecting plate; 13, X-axis bottom plate; 14, first guide rail; 15, first slider; 16, Y-axis bottom plate; 17, X-axis screw motor; 18, X-axis motor fixing seat; 19, X-axis motor nut connector; 20, second Guide rail; 21. Second slider; 22. Y-axis lead screw motor; 23. Y-axis motor fixing seat; 24. Y-axis motor nut connector; 25. Y-axis slider connector; 26. Collimator; 27. First dichroic mirror; 28. Objective lens; 29. ​​Achromatic lens; 30. Second dichroic mirror; 31. Camera; 32. Fluorescence detection component; 33. Guide rail fixing plate; 34. Third guide rail; 35. Third slider; 36. Slider connector; 37. Oiling needle; 38. Liquid circuit connector; 39. First oblique rib; 40. Vertical motor fixing plate; 41. Vertical lead screw motor; 42. Vertical motor lead screw connector; 43. Second oblique rib; 44. Laser. DETAILED DESCRIPTION

[0033] See also Figures 1 to 5As shown, according to an embodiment of the present invention, a dual-light path micro-droplet chip analyzer is provided, including a frame having an operating platform 1, several support columns 9 can be arranged below the operating platform 1 so that the operating platform 1 has a certain operating height, which is convenient for the operation of the operator, and the operating platform 1 is provided with a chip seat 4 capable of placing a micro-droplet chip 5, and the operating platform 1 is also provided with a first detection light path component 100 and a second detection light path component 200, and the micro-droplet chip 5 has at least two spaced channels, and the first detection light path component 100 can detect one of the two channels, and the second detection light path component 200 can detect the other of the two channels. In this technical solution, the first detection light path component 100 and the second detection light path component 200 are used at the same time to perform synchronous detection of multiple samples on two different channels on the micro-droplet chip 5, and they do not interfere with each other, and the received signals can be analyzed separately, which significantly reduces the chip detection time and improves the work efficiency.

[0034] In some embodiments, the first detection optical path component 100 includes an optical path component 110 and a position adjustment component 120, wherein the position adjustment component 120 can adjust the horizontal position and vertical position of the optical path component 110, that is, the position adjustment component 120 can adjust the actual position of the optical path component 110 to ensure that the position of the first detection optical path component 100 is more accurate, thereby ensuring the accuracy of the focus and detection results of the first detection optical path component 100. As a specific implementation, the structural composition of the second detection optical path component 200 is the same as that of the first detection optical path component 100. It should be noted that there may be some differences in the geometric dimensions of the specific components to ensure that the first detection optical path component 100 and the second detection optical path component 200 do not interfere with each other in arrangement and can meet the principle of simultaneously detecting two different channels.

[0035] As a specific implementation of the position adjustment component 120, the position adjustment component 120 includes an adjustment positioning block 10 and a support plate 11, wherein the support plate 11 is fixed to the operating platform 1 through the adjustment positioning block 10, and an X-axis position adjustment component (not labeled in the figure) is connected to the support plate 11, and a Y-axis position adjustment component (not labeled in the figure) is connected to the X-axis position adjustment component, and the Y-axis position adjustment component is connected to the optical path component 110, wherein the X-axis position adjustment component can adjust the horizontal position of the Y-axis position adjustment component and the optical path component 110, and the Y-axis position adjustment component can adjust the vertical position of the optical path component 110. In this technical solution, the position adjustment of the optical path component 110 in the vertical plane is finally achieved through the joint action of the X-axis position adjustment component and the Y-axis position adjustment component, and the structure is simple and compact. Specifically, the X-axis position adjustment component includes an X-axis base plate 13, on which an X-axis screw motor 17 is fixed through an X-axis motor fixing seat 18, and an X-axis motor nut connector 19 is threadedly connected to the screw of the X-axis screw motor 17, and the X-axis motor nut connector 19 is slidably connected to the first guide rail 14 through a first slider 15, and the first guide rail 14 is fixedly connected to the X-axis base plate 13; the Y-axis position adjustment component includes a Y-axis base plate 16, on which a Y-axis screw motor is fixed through a Y-axis motor fixing seat 23. The Y-axis motor 22 has a Y-axis motor nut connector 24 threadedly connected to the screw of the Y-axis screw motor 22. The Y-axis motor nut connector 24 is slidably connected to the second guide rail 20 through the second slider 21. The second guide rail 20 is fixedly connected to the Y-axis base plate 16. The second slider 21 is also connected to a Y-axis slider connector 25. The optical path assembly 110 is connected to the Y-axis slider connector 25 (specifically, it can be connected through the optical path connecting plate 12). The Y-axis base plate 16 is fixedly connected to the first slider 15.

[0036] In some embodiments, the optical path component 110 includes a laser 44, a collimator 26, a first dichroic mirror 27, an objective lens 28, an achromatic lens 29, a second dichroic mirror 30, a fluorescence detection component 32, and a camera 31. The laser light emitted by the laser 44 can pass through the collimator 26, be reflected from the first dichroic mirror 27 to the objective lens 28, and be focused onto the surface of the channel through the objective lens 28. A portion of the excited fluorescence enters from the objective lens 28, passes through the first dichroic mirror 27, passes through the achromatic lens 29 to reach the second dichroic mirror 30, a preset wavelength of light passes through the second dichroic mirror 30 to reach the fluorescence detection component 32, and another preset wavelength of light is refracted through the second dichroic mirror 30, enters the photosensitive surface of the camera 31, and displays the image of the channel on the computer screen.

[0037] The micro-droplet chip 5 includes at least four channels, and at least four channels are evenly spaced. The chip seat 4 can be translated in the horizontal direction. The first detection optical path component 100 and the second detection optical path component 200 are spaced along the translation path of the chip seat 4, and the spacing between the first detection optical path component 100 and the second detection optical path component 200 is at least twice the spacing between two adjacent channels. For example, the micro-droplet chip 5 has four channels. At this time, the spacing between the first detection optical path component 100 and the second detection optical path component 200 is twice the spacing between two adjacent channels. When the micro-droplet chip 5 has six channels, the spacing between the first detection optical path component 100 and the second detection optical path component 200 is three times the spacing between two adjacent channels, and so on. This method doubles the efficiency of sample detection.

[0038] Preferably, the operating platform 1 is fixedly connected with a chip motion rail 2, and the chip seat 4 is slidably connected to the chip motion rail 2 through a chip motion slider 3, so that the position of the micro-droplet chip 5 in the chip seat 4 can be converted by driving the movement of the chip seat 4. Specifically, the dual-light path micro-droplet chip analyzer also includes a chip horizontal drive component, which is fixed on the operating platform 1, and the chip horizontal drive component includes a horizontal screw motor 7, and a nut connector 8 is threadedly connected to the screw of the horizontal screw motor 7, and the nut connector 8 is also fixedly connected to the chip seat 4, so as to improve the movement automation of the chip seat 4.

[0039] Furthermore, the operating platform 1 is also connected to a crimping assembly 300, and a crimping plate 6 is also connected to the operating platform 1 at a position corresponding to the crimping assembly 300. The chip holder 4 has a crimping detection position in a first space formed between the crimping plate 6 and the crimping assembly 300, and a chip loading and unloading position outside the first space. When the chip holder 4 is in the crimping detection position, the crimping plate 6 and the crimping assembly 300 are respectively located at the upper and lower sides of the chip holder 4, so that the chip horizontal driving assembly can drive the chip The seat 4 drives the micro-droplet chip 5 to switch between the chip loading and unloading position and the crimping detection position. Specifically, when the micro-droplet chip 5 is in the chip loading and unloading position, the operator can load the micro-droplet chip 5 into the chip seat 4 or remove the micro-droplet chip 5 from the chip seat 4 manually or mechanically. At this time, since the chip seat 4 is outside the first space, there is no shielding component on it, that is, the micro-droplet chip 5 is placed vertically in the open space without avoiding any pillars, gantry and other mechanisms, and chip loading is simple and convenient.

[0040] In some embodiments, the crimping assembly includes a guide rail fixing plate 33, a vertical screw motor 41 is fixedly connected to the guide rail fixing plate 33 through a vertical motor fixing plate 40, the vertical screw motor 41 is connected to an oil injection needle 37 through a vertical motor screw connector 42, the oil injection needle 37 is slidably connected to the guide rail fixing plate 33 through a crimping sliding assembly, the guide rail fixing plate 33 is fixedly connected to the operating platform 1, and the oil injection needle 37 has a liquid path connector 38 connected to an external oil source. When the micro-droplet chip 5 needs to be crimped with oil, the micro-droplet core The sheet 5 is moved to the crimping detection position, at which time the vertical screw motor 41 is controlled to operate, and the oil injection needle 37 will drive the micro-droplet chip 5 above it to move closer, and the micro-droplet chip 5 will eventually be clamped by the oil injection needle 37 and the crimping plate 6 under the jacking action of the oil injection needle 37, and the oil injection needle 37 continues to jack up (move upward), and the columnar protrusion on the oil injection needle 37 (which has an oil hole) will be reliably inserted into the O-ring through hole in the oil interface on the micro-droplet chip 5, and the O-ring will produce a large deformation, forming a good seal, and the crimping is firm. It can be understood that the chip seat 4 should at least be constructed with a through hole that allows the oil injection needle 37 to pass through and contact the micro-droplet chip 5.

[0041] The crimping sliding assembly includes a third guide rail 34 fixedly connected to the guide rail fixing plate 33 and a third slider 35 slidably connected to the third guide rail 34. The third slider 35 is connected to the oil injection needle 37 through a slider connector 36 to ensure reliable and stable up and down linear movement of the oil injection needle 37. A first oblique rib 39 is also connected between the vertical motor fixing plate 40 and the guide rail fixing plate 33. A second oblique rib 43 is also connected to the guide rail fixing plate 33. The guide rail fixing plate 33 is fixedly connected to the operating platform 1 through the second oblique ribs 43 at both ends thereof to ensure that the vertical screw motor 41 is firmly installed.

[0042] According to an embodiment of the present invention, a control method for a dual-light path micro-droplet chip analyzer is also provided, which is used to control the dual-light path micro-droplet chip analyzer, comprising: injecting a micro-droplet chip 5 into a chip holder 4 at a chip loading and unloading position; controlling the chip horizontal drive assembly to operate the chip holder 4 to carry the micro-droplet chip 5 from the chip loading position to a crimping detection position, and when the micro-droplet chip 5 is at the crimping detection position, the first detection light path assembly 100 and the second detection light path assembly 200 are respectively opposite to two non-adjacent channels on the micro-droplet chip 5; controlling the pressure The connection assembly is operated to lift the micro-droplet chip 5 to contact the lower surface of the crimping plate 6, and the oil injection needle 37 in the crimping assembly is controlled to be crimped with the oil interface of the micro-droplet chip 5; the first detection optical path assembly 100 and the second detection optical path assembly 200 are controlled to be turned on at the same time, and the objective lenses 28 respectively provided by the first detection optical path assembly 100 and the second detection optical path assembly 200 are made to focus on the corresponding channels; the oil is controlled to enter the oil injection needle 37 and enter the corresponding channels of the micro-droplet chip 5 to complete the detection of the samples in the two non-adjacent channels. In this technical solution, multiple optical paths are used to simultaneously perform multiple sample synchronous detection on the same chip, which greatly shortens the detection time, facilitates chip loading, has high positioning accuracy, and has good chip crimping sealing.

[0043] Furthermore, after completing the detection of the samples in the two non-adjacent channels, the control method also includes: controlling the first detection optical path component 100 and the second detection optical path component 200 to close, and controlling the crimping component to reversely operate and return to zero; controlling the horizontal drive component to operate again to continue to move the chip holder 4 along the direction from the chip loading position to the crimping detection position by a displacement of a channel spacing; controlling the crimping component to operate to lift the micro-droplet chip 5 to contact the lower surface of the crimping plate 6, and controlling the oil injection needle 37 in the crimping component to be crimped with the oil interface of the micro-droplet chip 5; controlling the first detection optical path component 100 and the second detection optical path component 200 to open at the same time, and making the objective lenses 28 of the first detection optical path component 100 and the second detection optical path component 200 respectively complete the focusing of the corresponding channels; controlling the oil to enter the oil injection needle 37 and enter the corresponding channel of the micro-droplet chip 5 to complete the detection of the sample in the channel. In this way, by adjusting the horizontal position of the micro-droplet chip 5, other channels on the chip can be further detected, which is simple and efficient.

[0044] Furthermore, the control method also includes: after the sample detection in all channels in the micro-droplet chip 5 is completed, controlling the horizontal drive component to operate in reverse to move the chip holder 4 from the crimping detection position to the chip loading position, and unloading the micro-droplet chip 5 at the chip loading position. After the detection of all channels of a chip is completed, the chip is unloaded at the chip loading position, which is simple and convenient.

[0045] The technical solution of the present invention is further described below by taking the micro-droplet chip 5 having eight channels as an example.

[0046] The dual-optical path micro-droplet chip analyzer is first in the initial position, at which time the six motors (i.e., the two X-axis lead screw motors 17, the two Y-axis lead screw motors 22, the one horizontal lead screw motor 7, and the one vertical lead screw motor 41) are in their respective zero states (each zero position has a photoelectric switch), the nut connector 8 on the horizontal lead screw motor 7, and the chip holder 4 are in Figure 1 Far right shown.

[0047] The instrument starts to run, and relies on the detection device or human eyes to distinguish whether there is a micro-droplet chip 5 on the chip seat 4. If there is no micro-droplet chip 5 on the chip seat 4, the micro-droplet chip 5 is correctly placed in the limiting groove on the chip seat 4 by relying on a robot or human hand. At this time, the micro-droplet chip 5 is limited on the chip seat 4 by gravity, and the horizontal screw motor 7 is activated to move the nut connector 8, the chip seat 4, and the micro-droplet chip 5 to the first detection position. The vertical screw motor 41 is activated to drive the oil injection needle 37 to move from the zero point to the crimping position (that is, rise), completing the crimping of the micro-droplet chip 5. At this time, the pinhole on the oil injection needle 37 is aligned with the oil injection hole (that is, the oil interface) on the micro-droplet chip 5 and pressed tightly.

[0048] The external laser 44 is turned on, and the laser is emitted from the objective lens 28 of the first detection optical path component 100 and the second detection optical path component 200, and is focused on the focal plane of the objective lens 28. The X-axis lead screw motor 17 and the Y-axis lead screw motor 22 on the two detection optical path components are respectively operated, so that the laser focus falls on the channel of the micro-droplet chip 5, and the focal plane of the objective lens 28 coincides with the channel surface of the micro-droplet chip 5, that is, the image obtained by the camera 31 on the computer screen is the clearest, and the dual optical path is now focused on the channel of the micro-droplet chip 5. During the focusing process, the four motors in the two sets of detection optical path components are respectively operated, and fine-tuned according to the image algorithm to complete the focusing.

[0049] Afterwards, the oil enters the oil injection needle 37 through the liquid path connector 38, and enters the micro-droplet chip 5 through the oil injection hole of the micro-droplet chip 5, driving the droplets to be tested in the micro-droplet chip 5 to enter the channel and pass through the laser focus in sequence, such as Figure 4As shown, the fluorescent dye in the droplet is irradiated by laser and emits fluorescence, which passes through the objective lens 28, the first dichroic mirror 27, the achromatic lens 29, the second dichroic mirror 30 in sequence, and is focused at the fluorescence detection component 32. After being analyzed by the photoelectric analysis module, the signal value of the droplet to be detected is obtained, and a detection is completed.

[0050] After the first and fifth channel detections are completed, the external laser 44 is turned off, the vertical screw motor 41 returns to zero, the micro-droplet chip 5 leaves the lower surface of the crimping plate 6 under the action of gravity, and returns to the positioning groove of the chip seat 4, and the horizontal screw motor 7 is activated to drive the nut connector 8, the chip seat 4, and the micro-droplet chip 5 to move leftward along the X-axis (with a rotation of 1 / 40°). Figure 1 The orientation shown is for reference), the moving distance is the spacing distance between the two channels in the micro-droplet chip 5, the vertical screw motor 41 continues to move, driving the oiling needle 37 to move upward, and the crimping of the micro-droplet chip 5 is completed again, and the external laser 44 is turned on again, and the X-axis screw motor 17 and the Y-axis screw motor 22 on the first detection optical path component 100 and the second detection optical path component 200 refocus on the second channel and the sixth channel on the micro-droplet chip 5, and wait until the focus of the laser emitted by the optical path is exactly on the channel, and the focal plane coincides with the channel surface to complete the focusing. After the focusing is completed, oil is injected into the oiling needle 37 to push the droplets to be detected in the micro-droplet chip 5 to move into the channel, and make the droplets pass through the laser focus in turn, and the fluorescence emitted by the droplets is focused at the fluorescence detection component 32, and analyzed by the photoelectric analysis module to complete the signal detection.

[0051] Continue to complete the detection of the third and seventh channels and the fourth and eighth channels according to the above rules. When all eight channels in the micro-droplet chip 5 are detected, the external laser 44 is turned off, and the six motors on the dual-optical path micro-droplet chip analyzer are all returned to zero position. At this time, the micro-droplet chip 5 moves to the rightmost end of the instrument, and the micro-droplet chip 5 that has been detected is discarded to a designated recycling box or other chip placement device by hand or by a manipulator, and a new chip is placed to repeat the above action for detection.

[0052] The following describes the initial positioning adjustment method of the first detection optical path component 100 and the second detection optical path component 200:

[0053] See also Figure 1 , Figure 2 , Figure 4As shown, during the initial installation, the first detection optical path component 100 and the second detection optical path component 200 should be initially positioned by the adjustment and positioning blocks 10 located thereon, so that the axial center distance of the objective lens 28 respectively provided on the first detection optical path component 100 and the second detection optical path component 200 is roughly equal to the spacing between the first channel and the fifth channel on the micro-droplet chip 5; the crimping plate 6 has holes for the light of the two sets of detection optical path components to pass through. The specific adjustment method is as follows: first, the crimping plate 6 is fixed on the operating platform 1, and the horizontal screw motor 7 is driven to move the micro-droplet chip 5 to a suitable position below the crimping plate 6, so that the pinhole on the oil injection needle 37 can just align with the oil injection hole on the micro-droplet chip 5 during crimping, and the vertical screw motor 41 is actuated to move the oil injection needle 37 from the zero position to the crimping position, so that the oil injection needle 37 presses the micro-droplet chip 5, at which time the micro-droplet chip 5 rises upward from the placement groove in the chip seat 4 by about 2 mm, and the upper surface of the micro-droplet chip 5 The micro-droplet chip 5 is completely limited by fitting with the lower surface of the crimping plate 6, and the camera 31 in the first detection optical path component 100 is turned on. The X-axis screw motor 17 and the Y-axis screw motor 22 are fine-tuned until the image of the chip channel presented in the camera can be seen on the computer screen. The first detection optical path component 100 is fine-tuned along the X-axis direction so that the appropriate detection position is roughly located in the center of the screen image, and the adjustment and positioning block 10 on the first detection optical path component 100 is fixed to complete the position fixation of the first detection optical path component 100. Similarly, the second detection optical path component 200 is fixed.

[0054] The initial positioning adjustment method of the position adjustment component 120 is as follows:

[0055] See also Figure 1 , Figure 2 , the position adjustment component 120 and the support plate 11 also need to be adjusted and fixed when the instrument is initially installed. The function of the fine adjustment here is: when the optical path component 110 focuses on the channel on the micro-droplet chip 5, it needs to move downward from the zero point above until the focus falls on the channel on the micro-droplet chip 5. Due to the manufacturing error between different chips, the determination of this position determines the average distance that the optical path component 110 needs to move downward from the zero point position. The specific adjustment method of this position is: first, the first detection optical path component 100 is basically installed. At this time, the position adjustment component 120 and the support plate 11 are kept in a relatively loose threaded connection, and the X-axis screw motor 17 and the Y-axis screw motor 22 are fine-tuned until the image of the chip channel presented in the camera can be seen on the computer screen, and the absolute movement distance of the Y-axis screw motor 22 is checked. According to the distance, the position of the position adjustment component 120 on the support plate 11 is adjusted along the vertical groove on the support plate 11 to ensure the absolute movement distance of the Y-axis screw motor 22.

[0056] See also Figure 1 , Figure 5As shown, during the initial installation, the horizontal screw motor 7 moves to drive the micro-droplet chip 5 to move to the bottom of the crimping plate 6, and the vertical screw motor 41 manually fine-tunes to drive the oil injection needle 37 to move upward. By driving the horizontal screw motor 7 and the vertical screw motor 41, the two sets of pinholes on the oil injection needle 37 are just aligned with the first and fifth oil injection holes below the micro-droplet chip 5 through naked eye observation. At this time, the absolute position of the horizontal screw motor 7 is the first detection position to which the horizontal screw motor 7 needs to move from the zero position, and the first and fifth channels in the micro-droplet chip 5 can be detected at this position. Then, the vertical screw motor 41 continues to move, so that the oil injection needle 37 presses the micro-droplet chip 5 upward, so that when the external liquid path injects liquid into the oil injection needle 37, the oil does not leak from the connection between the oil injection needle 37 and the oil injection hole of the micro-droplet chip 5, and the micro-droplet chip 5 is not crushed. At this time, the absolute position of the vertical screw motor 41 is the position to which the vertical screw motor 41 moves from the zero position during crimping.

[0057] It is easy for those skilled in the art to understand that the above-mentioned advantageous methods can be freely combined and superimposed without conflict.

[0058] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A dual-optical path micro-droplet chip analyzer, characterized in that: The invention comprises a frame having an operating platform (1), wherein a chip seat (4) capable of placing a micro-droplet chip (5) is arranged on the operating platform (1), and a first detection optical path component (100) and a second detection optical path component (200) are also arranged on the operating platform (1), wherein the micro-droplet chip (5) has at least two spaced channels, and the first detection optical path component (100) can detect one of the two channels, while the second detection optical path component (200) can detect the other of the two channels; The first detection optical path component (100) comprises an optical path component (110) and a position adjustment component (120), wherein the position adjustment component (120) is capable of adjusting the horizontal position and the vertical position of the optical path component (110); The chip holder (4) is capable of being translated in a horizontal direction, the first detection optical path component (100) and the second detection optical path component (200) are arranged at intervals along the translation path of the chip holder (4), and the spacing between the first detection optical path component (100) and the second detection optical path component (200) is at least twice the spacing between two adjacent channels.

2. The dual-light path micro-droplet chip analyzer according to claim 1, characterized in that: The position adjustment component (120) comprises an adjustment positioning block (10) and a support plate (11); the support plate (11) is fixed to the operating platform (1) via the adjustment positioning block (10); an X-axis position adjustment component is connected to the support plate (11); the X-axis position adjustment component is connected to the Y-axis position adjustment component; the Y-axis position adjustment component is connected to the optical path component (110); the X-axis position adjustment component is capable of adjusting the horizontal position of the Y-axis position adjustment component and the optical path component (110); and the Y-axis position adjustment component is capable of adjusting the vertical position of the optical path component (110).

3. The dual-light path micro-droplet chip analyzer according to claim 1, characterized in that: The micro-droplet chip (5) comprises at least four channels, and the at least four channels are arranged at even intervals.

4. The dual-light path micro-droplet chip analyzer according to claim 3, characterized in that: The operating platform (1) is fixedly connected to a chip motion guide rail (2), and the chip seat (4) is slidably connected to the chip motion guide rail (2) via a chip motion slider (3).

5. The dual-light path micro-droplet chip analyzer according to claim 3, characterized in that: The operating platform (1) is also connected to a crimping assembly (300), and a crimping plate (6) is also connected to a position corresponding to the crimping assembly (300). The chip holder (4) has a crimping detection position located in a first space formed between the crimping plate (6) and the crimping assembly (300), and a chip loading and unloading position located outside the first space. When the chip holder (4) is in the crimping detection position, the crimping plate (6) and the crimping assembly (300) are located at the upper and lower sides of the chip holder (4), respectively.

6. The dual-light path micro-droplet chip analyzer according to claim 5, characterized in that: The crimping assembly comprises a guide rail fixing plate (33), a vertical screw motor (41) being fixedly connected to the guide rail fixing plate (33) via a vertical motor fixing plate (40), the vertical screw motor (41) being connected to an oil injection needle (37) via a vertical motor screw connecting piece (42), the oil injection needle (37) being slidably connected to the guide rail fixing plate (33) via a crimping sliding assembly, and the guide rail fixing plate (33) being fixedly connected to the operating platform (1).

7. A control method for a dual-light path micro-droplet chip analyzer, characterized in that: Used to control the dual-light path micro-droplet chip analyzer according to claim 6, comprising: Injecting the micro-droplet chip (5) into the chip holder (4) at the chip loading and unloading position; Controlling the operation of the chip horizontal drive assembly to move the chip holder (4) carrying the micro-droplet chip (5) from the chip loading position to the crimping detection position, wherein when the micro-droplet chip (5) is at the crimping detection position, the first detection optical path assembly (100) and the second detection optical path assembly (200) are respectively aligned with two non-adjacent channels on the micro-droplet chip (5); Controlling the operation of the crimping assembly to lift the micro-droplet chip (5) until it contacts the lower surface of the crimping plate (6), and controlling the oil injection needle (37) in the crimping assembly to crimp the oil interface of the micro-droplet chip (5); Controlling the first detection optical path component (100) and the second detection optical path component (200) to be turned on simultaneously, and causing the objective lenses (28) respectively provided in the first detection optical path component (100) and the second detection optical path component (200) to complete focusing on the corresponding channels; The oil is controlled to enter the oil injection needle (37) and then enter the corresponding channel of the micro-droplet chip (5), thereby completing the detection of the samples in the two non-adjacent channels.

8. The control method according to claim 7, characterized in that: After completing the detection of the samples in the two non-adjacent channels, the method further includes: Controlling the first detection optical path component (100) and the second detection optical path component (200) to be closed, and controlling the crimping component to reversely operate and return to zero; Controlling the horizontal driving assembly to operate again to move the chip holder (4) further along the direction from the chip loading position to the crimping detection position by a displacement of one channel pitch; Controlling the operation of the crimping assembly to lift the micro-droplet chip (5) until it contacts the lower surface of the crimping plate (6), and controlling the oil injection needle (37) in the crimping assembly to crimp the oil interface of the micro-droplet chip (5); Controlling the first detection optical path component (100) and the second detection optical path component (200) to be turned on simultaneously, and causing the objective lenses (28) respectively provided in the first detection optical path component (100) and the second detection optical path component (200) to complete focusing on the corresponding channels; The oil is controlled to enter the oil injection needle (37) and then enter the corresponding channel of the micro-droplet chip (5), thereby completing the detection of the sample in the channel.

9. The control method according to claim 7, characterized in that: Also includes: After the samples in all channels in the micro-droplet chip (5) are tested, the horizontal drive assembly is controlled to operate in reverse to move the chip holder (4) from the crimping test position to the chip loading position, and the micro-droplet chip (5) is removed at the chip loading position.

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