A method for disassembling forest biomass and its application

Abstract

The present invention relates to a method for disassembling forest biomass and its application. The method of the present invention changes the particle size of lignocellulose raw materials, couples with appropriate pretreatment methods for green disassembly, and then performs enzymatic hydrolysis to complete the splitting and conversion process of forest biomass. The present invention can effectively break the high-density anti-degradation barrier of forest biomass, reduce the pretreatment intensity, improve the utilization rate of component separation and extraction, and improve the pretreatment energy efficiency, thereby promoting enzymatic hydrolysis and subsequent conversion and utilization. The method utilizes the particle size effect and has the advantages of simple process, energy saving and low consumption, water saving and environmental protection, high sugar concentration, and low inhibitors.

Description

Technical Field

[0001] The invention belongs to the technical field of lignocellulose processing and relates to a method for disassembling forest biomass and application thereof. Background Art

[0002] As an important renewable energy source, forest biomass energy has the characteristics of large reserves, clean and renewable, and is one of the important renewable resources. Forest biomass is mainly composed of interwoven cellulose, hemicellulose and lignin. It is a dense and complex functional supramolecular body with a natural anti-degradation barrier. The natural anti-degradation barrier of forest biomass is one of the fundamental reasons for the difficulty in biorefining conversion. The pretreatment of forest biomass is crucial to changing the dense structure and splitting cellulose, hemicellulose and lignin. So far, the pretreatment of forest biomass is still one of the most expensive and critical unit operations in the biorefining process. It is the source of enzyme and microbial inhibition and determines the enzyme loading and the yield of enzyme hydrolysis products.

[0003] Forest biomass pretreatment refers to the destruction of the dense structure of raw materials through certain technical means, making them easier to be further degraded and utilized. Pretreatment is the entry point for high-value utilization of forest biomass components and the basis for component research. In the process of lignocellulose conversion and utilization, pretreatment plays a vital role. At present, the research on pretreatment mainly focuses on serious factors, lignin inhibition, catalysts and coupling integration. Studies have shown that too high pretreatment intensity will cause serious pollution, excessive energy consumption and excessive generation of inhibitors, affecting the entire biorefining process chain.

[0004] CN111995768A discloses a method for extracting wood fiber from tree branches and straws, which comprises crushing or slicing the raw materials into short strips and wood chips, soaking, crushing, and sieving to extract the wood fiber. However, the size of the raw materials mechanically treated by this method is at the centimeter level, and the wood fiber needs to be crushed and kneaded to collect the wood fiber, which is not applicable to a high-solid enzymatic hydrolysis system.

[0005] CN111154817A discloses a method for efficiently separating lignocellulose and enzymolysis using an ionic liquid-high boiling alcohol composite system, wherein the lignocellulose is crushed to less than 20 meshes, and after removing non-structural components, the lignocellulose is pretreated using an ionic liquid and high boiling alcohol composite system. However, this method only increases the effect of pretreatment by reducing particle size, and does not involve the effect of changes in particle size on the pretreatment effect.

[0006] CN106065080A discloses a dissolution-regeneration lignin extraction method, which uses crushing and ball milling pretreatment to change the size of lignocellulose particles and improve the efficiency of dissolution-regeneration lignin extraction of lignocellulose. However, the method is limited to micro-plant crushers and planetary ball mills for mechanically changing the particle size, and its purpose is to extract lignin, and it does not involve the effect of changes in particle size on the pretreatment effect.

[0007] It can be seen that although the above-mentioned pretreatment processes all crushed the raw materials, they did not involve the impact of changes in particle size on the pretreatment effect. The particle size, bulk density and mechanical properties of forest biomass raw materials are the decisive factors for pretreatment strength. Therefore, how to effectively reduce the pretreatment intensity by regulating the particle size and solve problems such as high cost, low efficiency and serious environmental pollution has become the dominant direction of pretreatment research. Summary of the invention

[0008] In view of the deficiencies in the prior art, the present invention provides a method for disassembling forest biomass and its application. The method of the present invention solves the problem that the pretreatment intensity of current forest biomass is too high due to its dense structure, achieves the purpose of breaking the high-density anti-degradation barrier, reduces the subsequent pretreatment intensity of lignocellulose, increases the utilization rate of component separation and extraction, improves the pretreatment energy efficiency, and has a simple process, energy saving and low consumption, and is easy to use for industrial application.

[0009] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0010] In a first aspect, the present invention provides a method for disassembling forest biomass, the method for disassembling forest biomass comprising: regulating the particle size of forest biomass raw materials, and then disassembling the forest biomass by coupling a pretreatment process, to obtain;

[0011] The particle size of the forest biomass raw material after regulation ranges from 100 μm to 3000 μm, for example, it can be 150 μm, 200 μm, 500 μm, 1000 μm, 1500 μm, 2000 μm, 2500 μm, etc.

[0012] Adjusting the particle size of forest biomass raw materials is a necessary step in pretreatment, but it is also a high-energy-consuming process. The particle size of forest biomass raw materials is an important parameter affecting the bulk density. In the present invention, by adjusting the particle size of forest biomass raw materials to 100μm-3000μm, within this size range, the pretreatment of wood cellulose can break the wrapping of lignin and hemicellulose on cellulose in the forest biomass cell structure, better remove lignin, degrade hemicellulose, change the crystal structure of cellulose, and at the same time increase the accessible internal surface area and looseness of cellulose, thereby increasing the reaction area between enzyme and substrate and significantly increasing the subsequent enzymatic hydrolysis efficiency.

[0013] Preferably, the forest biomass raw material includes any one of wood, bamboo or vine raw materials, or a combination of at least two of them.

[0014] Preferably, the wood includes any one of eucalyptus, poplar, pine, fir or locust wood, or a combination of at least two of them.

[0015] Preferably, the moisture content of the forest biomass raw material is ≤5%, for example, it can be 1%, 2%, 3%, 4%, etc.

[0016] In the present invention, by controlling the moisture content of the raw material, the particle size of the forest biomass can be controlled more quickly within a limited range, thereby more effectively reducing the pretreatment intensity of the lignocellulose.

[0017] Preferably, the step of regulating the particle size of the forest biomass raw material comprises: utilizing mechanical force to regulate the particle size of the forest biomass raw material by changing the intensity of the force.

[0018] Preferably, the source of the mechanical force includes any one of mechanical crushing, shearing, ball milling, disc milling or air flow crushing, or a combination of at least two of them.

[0019] Preferably, the operating time of the mechanical force is 30s-5h, for example, it can be 1min, 10min, 30min, 1h, 1.5h, 2h, 2.5h, 3h, 3.5h, 4h, 4.5h, etc.

[0020] Preferably, the operating temperature of the mechanical force is 10°C-80°C, for example, it can be 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, 50°C, 60°C, 70°C, 75°C, etc.

[0021] Preferably, the particle size of the forest biomass raw material after regulation is in the range of 500 μm-2000 μm.

[0022] Preferably, the method for disassembling forest biomass further comprises: subjecting the raw materials to freeze-thaw cycle treatment after adjusting the particle size of the forest biomass raw materials.

[0023] Preferably, the freeze-thaw cycle treatment includes: rehydrating the raw material after adjusting the particle size in a liquid medium, and then freezing it at -50 to -20°C (for example, it can be -45°C, -40°C, -35°C, -30°C, -25°C, etc.), and then thawing it at 20-40°C (for example, it can be 25°C, 28°C, 30°C, 33°C, 35°C, 38°C, etc.), and the freezing and thawing cycle is 2-8 times (for example, it can be 3 times, 4 times, 5 times, 6 times, 7 times).

[0024] Preferably, the solid-liquid volume ratio of the raw material to the liquid medium is 1:1-1:5, for example, it can be 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, etc.

[0025] Preferably, the rehydration treatment time is 20 min-50 min, for example, it can be 25 min, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min, etc.

[0026] As a preferred technical solution of the present invention, after the size of forest biomass particles is regulated, the raw materials are subjected to freeze-thaw cycles. The freeze-thaw cycles can increase the microcracks in the wood cellulose structure, increase the ion channels, and increase the diffusion coefficient, thereby further enhancing the component disassembly effect.

[0027] Preferably, the pretreatment process comprises any one of steam explosion, hydrothermal treatment, acid hydrolysis, alkaline hydrolysis or white rot fungus biodegradation, or a combination of at least two thereof.

[0028] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0029] In a second aspect, the present invention provides an application of the method for disassembling forest biomass as described in the first aspect in the degradation of lignocellulose.

[0030] In a third aspect, the present invention provides a lignocellulose enzymatic hydrolysis product, wherein the lignocellulose enzymatic hydrolysis product is obtained by subjecting the decomposition product obtained by the method for decomposing forest biomass according to the first aspect to enzymatic hydrolysis.

[0031] In the present invention, enzymatic hydrolysis is performed using the lignocellulose decomposition product obtained by the method of decomposing forest biomass, which can significantly reduce the amount of cellulase used, and effectively improve the enzymatic hydrolysis efficiency, increase the sugar concentration and reduce the enzymatic hydrolysis and subsequent fermentation costs.

[0032] Preferably, the solid content of the disassembled product in the enzymatic treatment system is 15-60%, for example, it can be 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, etc.

[0033] Preferably, the enzyme dosage in the enzymatic hydrolysis process is 5-20FPU / g DM, for example, it can be 6FPU / g DM, 8FPU / gDM, 10FPU / g DM, 12FPU / g DM, 15FPU / g DM, 17FPU / g DM, 19FPU / g DM, etc.

[0034] Preferably, the temperature of the enzymatic hydrolysis process is 45-55°C (for example, it can be 46°C, 47°C, 48°C, 49°C, 50°C, 51°C, 52°C, 53°C, 54°C, etc.), and the time is 24-96h (for example, it can be 30h, 36h, 42h, 48h, 54h, 60h, 72h, 84h, 90h, etc.).

[0035] In a fourth aspect, the present invention provides a use of the enzymatic hydrolysis product of lignocellulose as described in the third aspect in the preparation of bioethanol.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] 1. Mechanical action is used as the external power source for particle size adjustment, and the equipment used has a wide range of applications and is reusable.

[0038] 2. Through particle size control, the dense structure of forest biomass can be effectively broken, the pretreatment intensity can be reduced, and pollution, energy consumption and inhibitor generation can be reduced.

[0039] 3. The lignocellulose decomposition product obtained by the method of decomposing forest biomass can reduce the amount of cellulase during the enzymatic hydrolysis process, effectively improve the enzymatic hydrolysis efficiency, increase the sugar concentration, and take into account the downstream process, further reduce energy consumption and cost, and the reaction conditions are mild, the equipment requirements are low, and it is easy to industrialize production. DETAILED DESCRIPTION

[0040] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0041] Example 1

[0042] This embodiment provides a method for preparing a lignocellulose enzymatic hydrolysis product, the method comprising:

[0043] 1) removing impurities from the surface of the poplar wood chips and maintaining the moisture content at 3% after drying;

[0044] 2) crushing the poplar wood chips in step 1) in a knife mill for 20 min at a temperature of 25° C. to form wood powder with a particle size of 1500 μm, and collecting all the raw materials in the mill for pretreatment;

[0045] 3) The wood powder crushed in step 2) is subjected to steam explosion pretreatment, the steam explosion condition is 1.0 MPa, the maintenance time is 10 min, the solid-liquid separation is performed after the pretreatment, and the solid material is dried;

[0046] 4) Take 1 g of the solid material dried in step 3), add 15 FPU / g DM cellulase and citric acid-sodium citrate buffer with a pH of 4.8, and add the solid content to 40%. Place it in a 50°C water bath shaker and react at 120 rpm for 72 hours to collect the lignocellulose enzymatic hydrolysis product.

[0047] Example 2

[0048] This embodiment provides a method for preparing a lignocellulose enzymatic hydrolysis product, the method comprising:

[0049] 1) Remove surface impurities from eucalyptus chips and keep the moisture content at 2% after drying;

[0050] 2) ball milling the eucalyptus chips in step 1) in a planetary ball mill for 1 h at a temperature of 20° C. to form wood powder with a particle size of 100 μm, and collecting all the raw materials in the ball mill for pretreatment;

[0051] 3) The wood powder crushed in step 2) is subjected to hydrothermal pretreatment in an autoclave at a temperature of 140° C. for 30 min, solid-liquid separation is performed after the pretreatment, and the solid material is dried;

[0052] 4) Take 1 g of the solid material dried in step 3), add 5 FPU / g DM cellulase and citric acid-sodium citrate buffer with a pH of 4.8 and a solid content of 15%, place in a 45°C water bath shaker, react at 120 rpm for 96 hours, and collect the lignocellulose enzymatic hydrolysis product.

[0053] Example 3

[0054] This embodiment provides a method for preparing a lignocellulose enzymatic hydrolysis product, the method comprising:

[0055] 1) drying the bamboo slices, wherein the moisture content after drying is 5%;

[0056] 2) crushing the bamboo chips in step 1) in a toothed crusher for 10 min at a temperature of 50° C. to form bamboo powder with a particle size of 3000 μm, and collecting all the raw materials in the crusher for pretreatment;

[0057] 3) The crushed bamboo powder in step 2) is subjected to steam explosion pretreatment, the steam explosion condition is 1.0 MPa, the maintenance time is 15 min, and after the pretreatment, solid-liquid separation is performed, and the solid material is dried;

[0058] 4) Take 1 g of the solid material dried in step 3), add 20 FPU / g DM cellulase and citric acid-sodium citrate buffer with a pH of 4.8, with a solid content of 60%, place in a 55°C water bath shaker, react at 120 rpm for 36 hours, and collect the lignocellulose enzymatic hydrolysis product.

[0059] Example 4

[0060] This embodiment provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method is different from that in Example 1 only in that the moisture content of the poplar wood chips in step 1) is 10%, and the other steps and parameters are the same as those in Example 1.

[0061] Example 5

[0062] This example provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method is different from that in Example 1 only in that the particle size of the wood powder in step 2) is 500 μm, and the other steps and parameters are the same as those in Example 1.

[0063] Example 6

[0064] This example provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method is different from that in Example 1 only in that the particle size of the wood powder in step 2) is 2000 μm, and the other steps and parameters are the same as those in Example 1.

[0065] Example 7

[0066] This example provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method is different from that in Example 1 only in that the particle size of the wood powder in step 2) is 50 μm, and the other steps and parameters are the same as those in Example 1.

[0067] Example 8

[0068] This embodiment provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method is different from that in embodiment 1 only in that the particle size of the wood powder in step 2) is 5000 μm, and the other steps and parameters are the same as those in embodiment 1.

[0069] Example 9

[0070] This embodiment provides a method for preparing a lignocellulose enzymatic hydrolysis product, the method comprising:

[0071] 1) removing impurities from the surface of the poplar wood chips and maintaining the moisture content at 3% after drying;

[0072] 2) crushing the poplar wood chips in step 1) in a knife mill for 20 min at a temperature of 25° C. to form wood powder with a particle size of 1500 μm, and collecting all the raw materials in the mill for pretreatment;

[0073] 3) mixing the crushed raw material in step 2) with a 15% zinc chloride solution at a solid-liquid volume ratio of 1:3, rehydrating for 40 minutes, freezing the rehydrated raw material at -25°C for 6 hours, and then thawing it at 25°C for 6 hours, and performing freeze-thaw cycles for 5 times;

[0074] 4) The raw material after the freeze-thaw cycle in step 3) is pretreated by steam explosion, the steam explosion condition is 1.0 MPa, the maintenance time is 10 min, and after the pretreatment, solid-liquid separation is performed, and the solid material is dried.

[0075] 5) Take 1 g of the solid material dried in step 4), add 15 FPU / g DM cellulase and citric acid-sodium citrate buffer with a pH of 4.8, and add the solid content to 40%. Place it in a 50°C water bath shaker and react at 120 rpm for 72 hours to collect the lignocellulose enzymatic hydrolysis product.

[0076] Example 10

[0077] 1) Remove surface impurities from eucalyptus chips and keep the moisture content at 2% after drying;

[0078] 2) ball milling the eucalyptus chips in step 1) in a planetary ball mill for 1 h at a temperature of 20° C. to form wood powder with a particle size of 100 μm, and collecting all the raw materials in the ball mill for pretreatment;

[0079] 3) mixing the crushed raw material in step 2) with a 15% zinc chloride solution at a solid-liquid volume ratio of 1:1, rehydrating for 20 minutes, freezing the rehydrated raw material at -25°C for 6 hours, and then thawing it at 25°C for 6 hours, with 8 freeze-thaw cycles;

[0080] 4) subjecting the raw material after the freeze-thaw cycle in step 3) to hydrothermal pretreatment in an autoclave, the hydrothermal condition being 140° C. and the holding time being 30 min, performing solid-liquid separation after the pretreatment, and drying the solid material;

[0081] 5) Take 1 g of the solid material dried in step 4), add 5 FPU / g DM cellulase and citric acid-sodium citrate buffer with a pH of 4.8, with a solid content of 15%, place in a 45°C water bath shaker, react at 120 rpm for 96 hours, and collect the lignocellulose enzymatic hydrolysis product.

[0082] Embodiment 11

[0083] 1) drying the bamboo slices, wherein the moisture content after drying is 5%;

[0084] 2) crushing the bamboo chips in step 1) in a toothed crusher for 10 min at a temperature of 50° C. to form wood powder with a particle size of 3000 μm, and collecting all the raw materials in the crusher for pretreatment;

[0085] 3) mixing the crushed raw material in step 2) with a 15% zinc chloride solution at a solid-liquid volume ratio of 1:5, rehydrating for 50 minutes, freezing the rehydrated raw material at -25°C for 6 hours, and then thawing it at 25°C for 6 hours, and performing freeze-thaw cycles twice;

[0086] 4) The wood powder crushed in step 3) is subjected to steam explosion pretreatment, the steam explosion condition is 1.0 MPa, the maintenance time is 15 min, the solid-liquid separation is performed after the pretreatment, and the solid material is dried;

[0087] 5) Take 1 g of the solid material dried in step 4), add 20 FPU / g DM cellulase and citric acid-sodium citrate buffer with a pH of 4.8, with a solid content of 60%, place in a 55°C water bath shaker, react at 120 rpm for 36 hours, and collect the lignocellulose enzymatic hydrolysis product.

[0088] Comparative Example 1

[0089] This comparative example provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method is different from Example 1 only in that step 2) is not included, that is, the raw material treated in step 1) is directly pretreated, and the remaining steps and parameters are the same as those in Example 1.

[0090] Comparative Example 2

[0091] This comparative example provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method differs from Example 1 in that step 2) is not included, that is, the raw material treated in step 1) is directly pretreated, the intensity of the pretreatment is 3.0 MPa, the maintenance time is 50 min, and the remaining steps and parameters are the same as Example 1.

[0092] Comparative Example 3

[0093] This comparative example provides a method for preparing a lignocellulose enzymatic hydrolysis product. The method differs from Example 3 in that step 2) is not included, that is, the raw material treated in step 1) is directly pretreated, the pretreatment temperature is 200°C, the treatment time is 90 minutes, and the remaining steps and parameters are the same as Example 1.

[0094] The enzymolysis rates of Examples 1-11 and Comparative Examples 1-3 are shown in Table 1, and the enzymolysis rates are calculated as follows:

[0095] Enzymatic hydrolysis rate (glucose yield) = glucose production in enzymatic hydrolysis product (g) × 0.9 × 100% ÷ mass of cellulose in raw material (g)

[0096] Table 1: Enzymatic hydrolysis rate of lignocellulose

[0097]

[0098]

[0099] It can be seen from the effect data of Examples 1-3 that in the present invention, the particle size of forest biomass is regulated before lignocellulose pretreatment, so that the enzymatic hydrolysis efficiency of the pretreated lignocellulose at a high solid concentration is significantly improved. The research results show that the glucose yield of the pretreated lignocellulose obtained by the method of the present invention is as high as 85.6% after enzymatic hydrolysis at a high solid concentration. In Example 4, when the moisture content of the raw material is too high, the glucose yield after enzymolysis of lignocellulose is 78.3%, which is a decrease; from Examples 5-6, it can be seen that when the particle size is controlled at 500μm-2000μm, the glucose yield after enzymolysis of lignocellulose is better; from the data of Examples 7 and 8, it can be seen that when the particle size of the forest biomass in the present invention is too small, although the enzymolysis efficiency does not change significantly, the crushing time is longer and the cost is higher when the particle size is too small, and when the particle size is too large, the glucose yield after enzymolysis of lignocellulose is 70.3%. This is because when the particle size is too large, the accessible internal surface area and looseness of cellulose are too low, thereby reducing the enzymolysis efficiency; from Examples 9-11, it can be seen that adjusting the particle size to The controlled raw materials are then subjected to freeze-thaw cycle treatment, and the glucose yield is improved after the enzymatic hydrolysis of lignocellulose. This is because the freeze-thaw cycle treatment of the raw materials after the particles are regulated can make the disassembly effect of the pretreated lignocellulose better, thereby improving the enzymatic hydrolysis efficiency; from Comparative Example 1, it can be seen that when the lignocellulose is not subjected to particle regulation before pretreatment, the glucose yield after the enzymatic hydrolysis of lignocellulose is 55.4%, which shows a significant decrease. It can be seen that the particle size has a greater impact on the subsequent enzymatic hydrolysis efficiency; from Comparative Examples 2 and 3, it can be seen that although increasing the pretreatment intensity can improve the disassembly effect of lignocellulose and thereby improve the enzymatic hydrolysis efficiency, compared with the method of coupling pretreatment after particle size regulation, the enzymatic hydrolysis effect is still poor and the energy consumption is higher.

[0100] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a method for disassembling forest biomass and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0101] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0102] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A method for disassembling forest biomass, characterized in that: The method for disassembling forest biomass comprises: regulating the particle size of forest biomass raw materials, performing freeze-thaw cycle treatment, and then disassembling the forest biomass by coupling the pretreatment process, so as to obtain; After regulation, the particle size of forest biomass raw materials ranges from 1000μm to 1500μm; The moisture content of the forest biomass raw material is ≤5%; The step of regulating the particle size of the forest biomass raw material comprises: using mechanical force to regulate the particle size of the forest biomass raw material by changing the strength of the force; the source of the mechanical force comprises any one of mechanical crushing, shearing, ball milling, disc milling or air flow crushing, or a combination of at least two thereof; The pretreatment process is steam explosion; The freeze-thaw cycle treatment includes: rehydrating the raw material after adjusting the particle size in a liquid medium, then freezing it at -50 to -20°C, and then thawing it at 20 to 40°C, and the freezing and thawing cycle is repeated 2 to 8 times.

2. The method for disassembling forest biomass according to claim 1, characterized in that: The forest biomass raw material includes any one of wood, bamboo or vine raw materials, or a combination of at least two of them.

3. The method for disassembling forest biomass according to claim 2, characterized in that: The wood includes any one of eucalyptus, poplar, pine, fir or locust wood, or a combination of at least two of them.

4. The method for disassembling forest biomass according to claim 1, characterized in that: The operating time of the mechanical force is 1 min-1 h.

5. The method for disassembling forest biomass according to claim 1, characterized in that: The operating temperature of the mechanical force is 10°C-80°C.

6. The method for disassembling forest biomass according to claim 1, characterized in that: The solid-liquid volume ratio of the raw material to the liquid medium is 1:1-1:

5.

7. The method for disassembling forest biomass according to claim 1, characterized in that: The time of the rehydration treatment is 20min-50min.

8. Use of the method for disassembling forest biomass according to any one of claims 1 to 7 in the pre-degradation treatment of lignocellulose.

9. A lignocellulose enzymatic hydrolysis product, characterized in that: The lignocellulose enzymatic hydrolysis product is obtained by subjecting forest biomass to a disassembly product obtained by the method for disassembling forest biomass according to any one of claims 1 to 7 and then subjecting it to enzymatic hydrolysis.

10. The enzymatic hydrolysis product of lignocellulose according to claim 9, characterized in that The solid content of the disassembled product in the enzymatic treatment system is 15-60%.

11. The enzymatic hydrolysis product of lignocellulose according to claim 9, characterized in that The enzyme dosage in the enzymatic hydrolysis process is 5-20 FPU / g DM.

12. The lignocellulose enzymatic hydrolysis product according to claim 9, characterized in that The temperature of the enzymatic hydrolysis process is 45-55°C and the time is 24-96h.

13. Use of the enzymatic hydrolysis product of lignocellulose according to any one of claims 9 to 12 in the preparation of bioethanol.

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