A wound dressing with piezoelectric properties and its application

Abstract

The present invention relates to a wound dressing with piezoelectric properties and its application, belonging to the field of biomedical technology. The present invention provides a wound dressing with piezoelectric properties, the dressing comprising a PDMS film containing 20% ​​barium titanate. The present invention provides a preparation method of a wound dressing with piezoelectric properties and its application.

Description

Technical Field

[0001] The invention relates to a wound dressing with piezoelectric properties and application thereof, belonging to the technical field of biomedicine. Background Art

[0002] Skin trauma is common in accidental injuries or surgical operations, which can affect the integrity of the skin and increase the risk of infection. In traditional wound treatment, bandages and gauze are widely used as external protective barriers, but they cannot effectively promote wound healing. Recent studies have shown that wound repair is closely related to the endogenous electric field of the wound, which can promote cell migration and proliferation, which is crucial for many skin regeneration activities (such as collagen deposition, angiogenesis, and re-epithelialization). Piezoelectric stimulation therapy simulates and enhances the effect of the endogenous electric field of the wound by applying exogenous electrical stimulation to the wound surface. It has been used to promote skin wound healing, but its application in clinical surgery is often limited by the size of the equipment, and it is difficult to achieve real-time treatment. Therefore, the technical field urgently needs a biomaterial that is easy to use, can achieve real-time treatment, and can generate electrical stimulation at the wound for skin wound healing. Summary of the invention

[0003] The purpose of the present invention is to solve the technical problem of how to obtain a biomaterial that is easy to use, can achieve real-time treatment, and can generate electrical stimulation at the wound site for skin wound healing.

[0004] In order to achieve the purpose of solving the above-mentioned problem, the technical solution adopted by the present invention is to provide a wound dressing with piezoelectric properties, wherein the dressing includes a PDMS film containing 20% ​​barium titanate.

[0005] The present invention provides a method for preparing a wound dressing with piezoelectric properties, comprising the following steps:

[0006] Step 1: Add barium titanate powder into PDMS to obtain a solution with a mass volume ratio of 20%;

[0007] Step 2: Alkylate the surface of the silicon wafer;

[0008] Step 3: Use a spin coater to make a membrane, drip the solution obtained in step 1 on the silicon wafer, centrifuge it, and then put it in an oven to dry it into a membrane, mechanically peel the membrane from the surface of the silicon wafer, and then polarize the membrane to make the membrane have piezoelectric properties.

[0009] Preferably, the centrifugation condition in step 3 is 1500 rpm for 20 seconds.

[0010] Preferably, in step 3, the oven temperature is 80° C. and the placement time is greater than or equal to 2 hours.

[0011] Preferably, the polarization treatment in step 3 is polarization at 120° C. and 5 kV for 2 hours.

[0012] The present invention provides an application of a wound dressing with piezoelectric properties in a non-diagnostic method and a non-therapeutic method.

[0013] Preferably, the application includes application in preparing a wound dressing.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The present invention provides a wound dressing with piezoelectric properties, which provides a basic material for the subsequent further acquisition of medical materials that can be used conveniently and quickly, achieve real-time treatment, and facilitate rapid healing of skin wounds. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a graph showing the experimental results of the 20% barium titanate + PDMS film of the present invention being able to generate current.

[0017] Figure 2 This is a schematic diagram of the surgical site results of experimental rats of the present invention.

[0018] Figure 3 The figure is a schematic diagram of the striking device for striking experimental rats according to the present invention.

[0019] Figure 4 This is a picture of the healing of the back skin of two groups of experimental rats after surgery in the verification experiment of the present invention.

[0020] Figure 5 This is a histological staining picture of the healing of the back skin of two groups of experimental rats after surgery in the verification experiment of the present invention.

[0021] Figure 6 This is a comparison chart of the wound widths of two groups of experimental rats after surgery in the verification experiment of the present invention.

[0022] Figure 7 This is a comparison chart of the wound areas of two groups of experimental rats after surgery in the verification experiment of the present invention. DETAILED DESCRIPTION

[0023] In order to make the present invention more clearly understood, a preferred embodiment is described in detail with reference to the accompanying drawings as follows:

[0024] like Figure 1-7 As shown; the present invention provides a wound dressing with piezoelectric properties, the dressing comprising a PDMS film containing 20% ​​barium titanate.

[0025] The present invention provides a method for preparing a wound dressing with piezoelectric properties, comprising the following steps:

[0026] Step 1: Add barium titanate powder into PDMS to obtain a solution with a mass volume ratio of 20%;

[0027] Step 2: Alkylate the surface of the silicon wafer;

[0028] Step 3: Use a spin coater to make a membrane, drip the solution obtained in step 1 on the silicon wafer, centrifuge it, and then put it in an oven to dry it into a membrane, mechanically peel the membrane from the surface of the silicon wafer, and then polarize the membrane to make the membrane have piezoelectric properties.

[0029] The centrifugation condition in the above step 3 is 1500 rpm for 20 seconds.

[0030] In the above step 3, the oven temperature is 80° C. and the placement time is greater than or equal to 2 hours.

[0031] The polarization treatment in the above step 3 is to polarize at 120° C. and 5 kV for 2 hours.

[0032] The present invention provides an application of a wound dressing with piezoelectric properties in a non-diagnostic method and a non-therapeutic method.

[0033] The above application includes application in preparing wound dressing.

[0034] Example

[0035] 1. Material preparation:

[0036] Fabrication of membranes by spin coating:

[0037] Step 1: Add barium titanate powder to PDMS (Polydimethylsiloxane) (10:1 PDMS prepolymer and curing agent) to obtain a solution with a mass volume ratio of 20%, (ie, hereinafter: 20% barium titanate + PDMS solution)

[0038] Step 2: Alkylate the surface of the silicon wafer, which is conducive to mechanical peeling of the membrane;

[0039] Step 3: Add 20% barium titanate + PDMS solution on the silicon wafer at 1500rpm for 20 seconds, then put it into an oven at 80℃ for 2 hours to form a thin film, and then polarize the film at 120℃ and 5kv for 2 hours to make the film have piezoelectric properties; the obtained film is a 20% barium titanate + PDMS film.

[0040] The obtained thin film material can generate electric current under mechanical stimulation. Figure 1 (shown)

[0041] 2. Animal experiment verification:

[0042] All materials and surgical instruments were sterilized to prevent wound infection. Three-month-old SD rats were selected to evaluate the effect of piezoelectric material patch on skin wound healing.

[0043] The SD rats were divided into a control group (PDMS film patch group) and an experimental group (20% barium titanate + PDMS film patch group).

[0044] Before the operation, the rats were anesthetized, and the backs of the rats were depilated with depilatory cream. The back skin was disinfected with alcohol, and the full thickness skin layer of the back of the rats was removed (a longitudinal incision with an area of ​​3x8mm). The left side of the rats was used as the control group (the incision was covered with a PDMS film patch alone), and the right side was used as the experimental group (the incision was covered with a 20% barium titanate + PDMS film patch). The two groups of materials were pasted on the wounds, and then covered with a transparent film and an elastic bandage to protect the treatment. The patches were replaced once a day (such as Figure 2 All rats were housed individually.

[0045] Mechanical stimulation tapping device (such as Figure 3 ) can apply external force to the piezoelectric material to stimulate the generation of exogenous voltage on the wound surface. Due to the piezoelectric properties of barium titanate, the low-frequency pulse voltage generated by local mechanical energy can enhance the endogenous electric field on the wound surface and promote skin regeneration activities (such as cell behavior and growth factor expression). Figure 3 As shown in the figure, the beating device generates mechanical stimulation under adjustable voltage, the beating frequency is 10HZ, and the end is composed of a 50*50*3mm silicone block. The rat is fixed on the table so that the upper silicone block hits the wound patch on the rat's back twice a day, each time for 10 minutes. (As shown in the figure, the beating device generates mechanical stimulation under adjustable voltage, the beating frequency is 10HZ, and the end is composed of a 50*50*3mm silicone block. The rat is fixed on the table so that the upper silicone block hits the wound patch on the rat's back twice a day, each time for 10 minutes. Figure 3 );

[0046] The healing of the rat back skin was observed on the 7th, 10th and 14th days after surgery (eg Figure 4 ) and histological staining (such as Figure 5 ), it can be clearly observed that the wound healing was better in the 20% barium titanate + PDMS film patch group. Figure 6 , Figure 7 The figure shows the comparison of wound width and wound area between the two groups on the 7th and 14th days after surgery. It can also be clearly observed that the wound healing was better in the 20% barium titanate + PDMS film patch group.

[0047] The above is only a preferred embodiment of the present invention, and is not any formal or substantial limitation of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and supplements without departing from the present invention, and these improvements and supplements should also be regarded as the protection scope of the present invention. Any technician familiar with this profession, without departing from the spirit and scope of the present invention, can make some changes, modifications and equivalent changes made by using the technical content disclosed above, which are equivalent embodiments of the present invention; at the same time, any changes, modifications and evolutions of any equivalent changes made to the above embodiments based on the essential technology of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. Application of a wound dressing with piezoelectric properties in the preparation of a band-aid, characterized in that: The preparation method of the wound dressing comprises the following steps: Step 1: Add barium titanate powder into PDMS to obtain a solution with a mass volume ratio of 20%; Step 2: Alkylate the surface of the silicon wafer; Step 3: Use a spin coater to make a membrane, drip the solution obtained in step 1 on the silicon wafer, centrifuge it, and then put it in an oven to dry it into a membrane, mechanically peel the membrane from the surface of the silicon wafer, and then polarize the membrane to make the membrane have piezoelectric properties.

2. The use according to claim 1, characterized in that: The centrifugation condition in step 3 is 1500 rpm for 20 seconds.

3. The use according to claim 1, characterized in that: In step 3, the oven temperature is 80° C. and the placement time is greater than or equal to 2 hours.

4. The use according to claim 1, characterized in that: The polarization treatment in step 3 is to polarize at 120° C. and 5 kV for 2 hours.

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