Pesticide composition for increasing frost resistance of citrus

Abstract

The present invention is a pesticide composition for increasing the antifreeze property of citrus, and solves the problem that it is difficult to protect the fruits and leaves of citrus after the ambient temperature is lower than 2-10°C. The present invention comprises the following components by weight percentage: 0.5-15% of soybean oligopeptide, 0.5-15% of yield-increasing amine, 5-30% of aloe vera extract, 0.5-8% of surfactant, 0.5-8% of cosolvent, and the balance of water. Compared with the control example, the pesticide composition for increasing the antifreeze property of citrus has significant differences in improving the leaf drop rate, fruit drop rate, and antifreeze level.

Description

Technical Field

[0001] The invention relates to a pesticide composition for increasing the frost resistance of citrus, belonging to the field of plant growth regulators. Background Art

[0002] Citrus is an important economic crop in the southern region. It is generally planted in the open air without protective measures such as greenhouses. It is obviously affected by natural factors. Every winter, due to the southward movement of cold waves and cold air, the southern region is often accompanied by a sharp drop in temperature. The temperature drop is relatively large. In southern regions such as Jiangxi and other places, the night temperature sometimes drops to between -2 and -10℃ around January each year, and then the temperature quickly rises to between 8 and 15℃. This has a great impact on citrus varieties whose growth cycle needs to span the winter, such as Wogan and Babagan. At present, there are two conventional methods of pesticide treatment: the first is to rely on spraying plant growth regulators brassinolide and abscisic acid for regulation, but this method is only more beneficial for fields where the night temperature is above 0℃ for a short time, but the natural environment with a temperature below 0℃ has a weakened effect; the second is to spray a mixture of urea, zinc sulfate and potassium dihydrogen phosphate for antifreeze, but when the ambient temperature is below 0℃, it is difficult to protect the fruits and leaves of citrus. Summary of the invention

[0003] The purpose of the present invention is to provide a pesticide composition which can significantly increase the temperature range of citrus antifreeze tolerance, greatly reduce the leaf drop rate and fruit drop rate, and is convenient to use, safe and environmentally friendly.

[0004] The objective of the present invention is achieved in this way:

[0005] The pesticide composition for increasing the frost resistance of citrus fruits comprises the following components by weight percentage:

[0006] Soybean oligopeptide 0.5~15%,

[0007] Increase amine production by 0.5~15%,

[0008] Aloe vera extract 5~30%,

[0009] Surfactant 0.5~8%,

[0010] Solvent 0.5~8%,

[0011] Make up the remaining water.

[0012] The preparation method of the aloe vera extract comprises the following steps: freezing the aloe vera to a vitrified state by liquid nitrogen, crushing the aloe vera by a pulverizer, and then performing reduced pressure extraction in an extraction tank according to a ratio of ethanol: glycerol: frozen and crushed aloe vera of 5:5:1, wherein the pressure is controlled between -0.02 and -0.04 MPa, the temperature is controlled between 40 and 50°C, the stirring time is 2 to 4 hours, and after the extraction is completed, the temperature is cooled to room temperature, and the filtrate is collected by filtration to obtain the aloe vera extract, and the filter cake is used as a matrix of a biological organic fertilizer for other purposes.

[0013] The surfactant is at least one of sodium dodecylbenzene sulfonate, lauryl betaine, and fatty alcohol polyoxyethylene ether.

[0014] The co-solvent is at least one of citric acid monohydrate and tartaric acid.

[0015] The pesticide composition for increasing the frost resistance of citrus is a liquid.

[0016] The pesticide composition for increasing the frost resistance of citrus fruits comprises the following components by weight percentage:

[0017] Soybean oligopeptide 5~10%,

[0018] Increase amine production by 6~12%,

[0019] Aloe vera extract 10~25%,

[0020] Surfactant 2~6%,

[0021] Solvent 3~8%,

[0022] Make up the remaining water.

[0023] Spray 2-4 times 30-40 days before New Year's Day every year. By spraying in advance, the accumulation of citrus photosynthesis is enhanced and the anti-freeze ability of citrus protoplasts is trained.

[0024] The preparation method of the invention comprises the following steps: firstly, dissolving the auxiliary solvent completely with water, then adding the yield-increasing amine, heating and dissolving completely, then sequentially adding the surfactant, soybean oligopeptide and aloe vera extract, and stirring until completely dissolved.

[0025] Soybean oligopeptides are small molecule soybean oligopeptide powders obtained by enzymatic hydrolysis, separation, purification and drying of non-GMO soybean protein isolate. Its amino acid composition is the same as that of soybean protein, with a good balance of essential amino acids, rich content, and a molecular weight concentrated between 300-700 Daltons. Compared with ordinary amino acids, oligopeptides are more easily absorbed and utilized by plants, and compared with other oligopeptides and amino acids, soybean oligopeptides have a stronger calcium retention capacity in the body. Calcium, as the second messenger in plant cells, has a significant effect on enhancing the antifreeze ability of plants. Traditional calcium supplementation methods have a poor duration of effect. Through the action of soybean oligopeptides, calcium ions can enter cells along the concentration gradient, improve the antifreeze ability of cell protoplasts, and thus enhance the antifreeze ability of citrus.

[0026] The photosynthesis-increasing amine can significantly enhance the photosynthesis of plants. It is absorbed by the stems and leaves of plants and directly acts on the cell nucleus in the plants, enhancing the activity of enzymes and leading to an increase in the content of plant sap, oil and fat, thereby enhancing the frost resistance of plants.

[0027] Aloe vera extract is rich in aloe polysaccharides, anthraquinones, aloin, aloe-emodin, aloe-emodin phenol, aloe saponin, aloin, aloin, aloemycin and other active ingredients. After spraying, aloe polysaccharides are beneficial to promote the absorption and utilization of nutrients by citrus, and at the same time, synergistically promote the thickening of the wax layer of citrus leaves and fruits with other extracted ingredients, playing the role of forming a protective film. Anthraquinones help repair citrus damage and can synergistically enhance the frost resistance of citrus.

[0028] Enhancing the frost resistance of citrus through exogenous drugs is a gradual process. First, it is necessary to enhance the photosynthesis of citrus in advance through the use of yield-enhancing amines, and increase the content of cell sap and lipids in citrus leaves and fruits. Secondly, through the action of soybean oligopeptides, extracellular calcium ions are complexed into cells. Calcium, as the second messenger in plant cells, improves the frost resistance of protoplasts that have not been trained in low temperatures in the early stage when there is a large temperature difference in the early stage. The frost resistance of citrus can be effectively enhanced by spraying in advance. At the same time, the aloe polysaccharides in the Curaçao aloe extract promote the absorption and utilization of yield-enhancing amines and soybean oligopeptides by citrus, and then cooperate with other aloe extracts to thicken the wax layer of citrus leaves and fruits, ultimately playing an anti-freeze and cold-resistant role.

[0029] The pesticide composition for increasing the frost resistance of citrus fruits can be sprayed in advance to enhance the accumulation of photosynthesis in citrus fruits, exercise the frost resistance of protoplasts in citrus fruits, significantly increase the frost resistance temperature range of citrus fruits, prevent leaf and fruit drop, and significantly increase the economic benefits of growers. It has good use effect, is easy to apply, and is low in toxicity and environmentally friendly.

[0030] The advantages of the present invention are as follows:

[0031] The pesticide composition B of the present invention that increases the frost resistance of citrus can significantly improve the frost resistance of citrus, reduce the leaf and fruit drop rates of citrus (see the test example), and can significantly increase the economic benefits of citrus growers when extreme freezing weather comes. If the fruit drop rate of citrus reaches more than 10%, the impact on the yield is relatively large. During actual planting, the fruit drop rate of citrus must be strictly controlled to ensure stable economic benefits. Compared with the control example, the pesticide composition of the present invention that increases the frost resistance of citrus has significant differences in improving the leaf drop rate, fruit drop rate, and frost resistance level. Through comparative tests in two places, the effect of the pesticide composition of the present invention that increases the frost resistance of citrus is very stable, has a significant effect on improving the frost resistance of citrus fruits, and can significantly increase the economic benefits of growers. DETAILED DESCRIPTION

[0032] Embodiment 1:

[0033] The pesticide composition for increasing the frost resistance of citrus in this embodiment 1 includes, by weight percentage: 0.5% soybean oligopeptide, 0.5% yield-increasing amine, 5% aloe vera extract, 0.5% sodium dodecylbenzenesulfonate, 1% citric acid monohydrate, and the balance water. The preparation method of the aloe vera extract is: freezing the aloe vera to a vitrified state by liquid nitrogen, then crushing it by a pulverizer, and then performing reduced pressure extraction in an extraction tank according to the ratio of ethanol: glycerol: aloe vera frozen crushed material 5:5:1, the pressure is controlled between -0.02 and -0.04Mpa, the temperature is controlled at 40 to 50°C, the stirring time is 2 to 4h, and after the extraction is completed, it is cooled to room temperature, and the filtrate is collected by filtration, which is the aloe vera extract, and the filter cake is used as a matrix of biological organic fertilizer for other purposes.

[0034] Embodiment 2:

[0035] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 2 comprises, by weight percentage: 1% soybean oligopeptide, 1% yield-increasing amine, 6% aloe vera extract, 0.5% sodium dodecylbenzenesulfonate, 0.5% lauryl betaine, 1% citric acid monohydrate, 0.5% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0036] Embodiment 3:

[0037] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 3 comprises, by weight percentage: 5% soybean oligopeptide, 3% yield-increasing amine, 8% aloe vera extract, 0.5% sodium dodecylbenzenesulfonate, 0.5% lauryl betaine, 1% fatty alcohol polyoxyethylene ether, 1% citric acid monohydrate, 2% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0038] Embodiment 4:

[0039] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 4 comprises, by weight percentage: 8% soybean oligopeptide, 5% yield-increasing amine, 10% aloe vera extract, 1% sodium dodecylbenzenesulfonate, 0.5% lauryl betaine, 1% fatty alcohol polyoxyethylene ether, 3% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that in Example 1.

[0040] Embodiment 5:

[0041] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 5 comprises, by weight percentage: 9% soybean oligopeptide, 6% yield-increasing amine, 15% aloe vera extract, 1% sodium dodecylbenzenesulfonate, 1% lauryl betaine, 1% fatty alcohol polyoxyethylene ether, 3% tartaric acid, 3% citric acid monohydrate, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0042] Embodiment 6:

[0043] The pesticide composition for increasing the frost resistance of citrus fruits in Example 6 comprises, by weight percentage: 10% soybean oligopeptide, 8% yield-increasing amine, 20% aloe vera extract, 4% lauryl betaine, 1% fatty alcohol polyoxyethylene ether, 6% citric acid monohydrate, and the balance water. The preparation method of aloe vera extract is the same as that of Example 1.

[0044] Embodiment 7:

[0045] The pesticide composition for increasing the frost resistance of citrus fruits in Example 7 comprises, by weight percentage: 11% soybean oligopeptide, 10% yield-increasing amine, 21% aloe vera extract, 4% lauryl betaine, 1.5% fatty alcohol polyoxyethylene ether, 4% citric acid monohydrate, 3% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0046] Embodiment 8:

[0047] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 8 comprises, by weight percentage: 12% soybean oligopeptide, 11% yield-increasing amine, 22% aloe vera extract, 0.5% sodium dodecylbenzenesulfonate, 4% lauryl betaine, 1.5% fatty alcohol polyoxyethylene ether, 4.5% citric acid monohydrate, 3% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0048] Embodiment 9:

[0049] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 9 comprises, by weight percentage: 13% soybean oligopeptide, 13% yield-increasing amine, 25% aloe vera extract, 1% sodium dodecylbenzenesulfonate, 4% lauryl betaine, 1.5% fatty alcohol polyoxyethylene ether, 3.5% citric acid monohydrate, 4% tartaric acid, and the balance water. The preparation method of aloe vera extract is the same as that in Example 1.

[0050] Embodiment 10:

[0051] The pesticide composition for increasing the frost resistance of citrus fruits in Example 10 comprises, by weight percentage: 14% soybean oligopeptide, 14% yield-increasing amine, 27% aloe vera extract, 1% sodium dodecylbenzenesulfonate, 4.5% lauryl betaine, 1.5% fatty alcohol polyoxyethylene ether, 4% citric acid monohydrate, 4% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0052] Embodiment 11:

[0053] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 11 comprises, by weight percentage: 14% soybean oligopeptide, 14% yield-increasing amine, 28% aloe vera extract, 1% sodium dodecylbenzenesulfonate, 4% lauryl betaine, 2% fatty alcohol polyoxyethylene ether, 3.5% citric acid monohydrate, 4% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that in Example 1.

[0054] Embodiment 12:

[0055] The pesticide composition for increasing the antifreeze property of citrus fruits in Example 12 comprises, by weight percentage: 15% soybean oligopeptide, 15% yield-increasing amine, 30% aloe vera extract, 2% sodium dodecylbenzenesulfonate, 4% lauryl betaine, 1.5% fatty alcohol polyoxyethylene ether, 4% citric acid monohydrate, 4% tartaric acid, and the balance water. The preparation method of the aloe vera extract is the same as that of Example 1.

[0056] Test example

[0057] Comparative Example B is the pesticide composition for increasing the frost resistance of citrus fruits of Example 6, which is prepared according to the following weight percentages: 10% soybean oligopeptide, 8% yield-increasing amine, 20% aloe vera extract, 4% lauryl betaine, 1% fatty alcohol polyoxyethylene ether, 6% citric acid monohydrate, and the balance water.

[0058] The 12 comparative examples AL, including the pesticide composition (B) for increasing the frost resistance of citrus obtained in the above Example 6, were tested and compared on mandarin oranges.

[0059] The test was conducted in Fuzhou City, Jiangxi Province and Wogan Industrial Park, Shangrao City. 72 citrus trees with the same growth conditions were selected in the industrial park, 12 treatments were counted, and the comparative test was repeated three times, randomly arranged, and the twelve comparisons were: A: clean water control; B: 1000 times diluted in Example 6 (100ppm soybean oligopeptide + 80ppm yield-increasing amine + 200ppm aloe vera extract); C: 10% soybean oligopeptide diluted 1000 times (100ppm soybean oligopeptide); D: 8% yield-increasing amine diluted 1000 times (80ppm yield-increasing amine); E: 20% aloe vera extract diluted 1000 times (200ppm aloe vera extract); F: 10%% soybean oligopeptide + 8% yield-increasing amine diluted 1000 times (100ppm soybean oligopeptide + 80ppm yield-increasing amine); G: 10% soybean oligopeptide + 20% aloe vera extract diluted 1000 times (100ppm soybean oligopeptide + 200ppm aloe vera extract); H: 8% yield-increasing amine + 20% aloe vera extract diluted 1000 times (80ppm yield-increasing amine + 200ppm aloe vera extract); I: commercially available 0.004% brassinolide diluted 1000 times; J: commercially available 0.1% inducible diluted 333 times; K: commercially available 160g / L calcium fertilizer diluted 1000 times; L: 0.3% zinc sulfate + 0.3% urea + 0.3% potassium dihydrogen phosphate solution.

[0060] The above comparative test was repeated three times, and the medication period was November 25, 2020. The leaf surface was evenly sprayed three times, with a safety interval of 15 days. The statistics were counted on January 30, 2021, and the tests were conducted in two places. Statistical analysis was performed according to the citrus frost injury classification standard.

[0061] Test results:

[0062] 1. Effects of different treatments on the frost resistance of Wogan in Fuzhou and Shangrao, Jiangxi Province (see Table 1)

[0063] Table 1 Effects of different treatments on frost resistance of Wogan (2020)

[0064]

[0065] In January 2021, Fuzhou City and Shangrao City in Jiangxi Province experienced freezing weather below 0°C, and there was also rain and snow weather as low as -5°C. As can be seen from Table 1, the pesticide composition B of the present invention, which increases the frost resistance of citrus, can significantly improve the frost resistance of citrus, with a frost damage level of zero, and very low leaf and fruit drop rates. Compared with other comparison examples, there is a significant difference, which can greatly increase the economic benefits of growers and ensure that the damage to fruit trees is minimized. Citrus is a perennial economic crop. When evaluating the economic benefits of citrus, in addition to considering the leaf fall and fruit drop rates, it is also necessary to consider whether the citrus trees are damaged, that is, the frostbite level of the citrus, and whether it affects the growth of the next year. If the fruit drop rate of citrus reaches more than 10%, the impact on the current year's yield is relatively large. In actual planting, the fruit drop rate of citrus must be strictly controlled to ensure stable economic benefits. The pesticide composition for increasing the frost resistance of citrus of the present invention can solve the damage to the tree body caused by extreme freezing weather, without reducing production or damage. Compared with the control example, there are significant differences in improving the leaf fall rate, fruit drop rate, and frost resistance level. Through comparative tests in two places, the effect of the pesticide composition for increasing the frost resistance of citrus of the present invention is very stable, has a significant effect on improving the frost resistance of citrus fruits, and can significantly increase the economic benefits of growers.

[0066] The above embodiments are further explanations of the above contents of the present invention, but it should not be understood that the scope of the above subject matter of the present invention is limited to the above embodiments. All technologies realized based on the above contents belong to the scope of the present invention.

Claims

1. A pesticide composition for increasing the frost resistance of citrus fruits, characterized in that: The following components are included by weight percentage: Soybean oligopeptide 0.5~15%, Increase amine production by 0.5~15%, Aloe vera extract 5~30%, Surfactant 0.5~8%, Solvent 0.5~8%, Make up the remaining water. The preparation method of the aloe vera extract comprises the following steps: freezing the aloe vera to a vitrified state by liquid nitrogen, crushing the aloe vera by a pulverizer, and then performing reduced pressure extraction in an extraction tank according to a ratio of ethanol: glycerol: aloe vera frozen and crushed materials of 5:5:1, wherein the pressure is controlled between -0.02 and -0.04 MPa, the temperature is controlled between 40 and 50°C, the stirring time is 2 to 4 hours, and after the extraction is completed, the temperature is lowered to room temperature, and the filtrate is collected by filtration to obtain the aloe vera extract, and the filter cake is used as a matrix of a biological organic fertilizer for other purposes. The surfactant is at least one of sodium dodecylbenzene sulfonate, lauryl betaine and fatty alcohol polyoxyethylene ether. The co-solvent is at least one of citric acid monohydrate and tartaric acid. The pesticide composition for increasing the frost resistance of citrus is a liquid.

2. The pesticide composition for increasing the frost resistance of citrus according to claim 1, characterized in that: The above-mentioned pesticide composition for increasing the frost resistance of citrus fruits comprises the following components by weight percentage: Soybean oligopeptide 5~10%, Increase amine production by 6~12%, Aloe vera extract 10~25%, Surfactant 2~6%, Solvent 3~8%, Make up the remaining water.

3. The pesticide composition for increasing the frost resistance of citrus according to claim 1, characterized in that: The preparation method is as follows: firstly, the auxiliary solvent is completely dissolved with water, then the yield-increasing amine is added, heated to completely dissolve, and then the surfactant, soybean oligopeptide and aloe vera extract are added in sequence, and stirred until completely dissolved.

4. The method for using the pesticide composition for increasing the frost resistance of citrus fruits according to claim 1, characterized in that: Spray 2-4 times 30-40 days before New Year's Day every year.

Images