Analgesic and anti-inflammatory gel and preparation method thereof

Abstract

The invention relates to the technical field of medical gels, and proposes an analgesic and anti-inflammatory gel and a preparation method thereof, comprising 6-8 parts of Chinese medicine additives, 0.5-1 parts of polyvinyl caprolactam, 2-4 parts of oxidized sodium alginate, 1-3 parts of oligomaltose, 1-2 parts of histidine, 0.05-0.1 parts of silymarin, 3-6 parts of moisturizer, 0.2-0.4 parts of preservative, and 75-85 parts of water. The technical scheme solves the problems of poor antibacterial effect and long healing time of gel in the prior art.

Description

Technical Field

[0001] The present invention relates to the technical field of medical gels, and in particular to an analgesic and anti-inflammatory gel and a preparation method thereof. Background Art

[0002] With the continuous development of social economy and the improvement of people's living standards, more and more patients choose to be hospitalized for treatment of dentofacial deformity and orthognathic surgery. Dentofacial deformity refers to the abnormal volume and shape of the jaw and the relationship between the upper and lower jaws and other bones of the craniofacial region caused by abnormal jaw development, and the accompanying abnormal jaw-tooth relationship and oral and maxillofacial system function and facial morphology.

[0003] Before orthognathic surgery, some patients experience swollen and painful gums, which seriously affect their preoperative psychology. In addition, after orthognathic surgery, local swelling and high oral sensitivity will affect the patient's quality of life to a certain extent. Not only is it accompanied by pain, but severe cases will also change their appearance for a certain period of time. Therefore, an analgesic and anti-inflammatory gel is needed to reduce the patient's pain and local swelling before and after the operation, achieve the purpose of inhibiting bacteria and inflammation during and after the operation, and accelerate the healing of postoperative wounds. Summary of the invention

[0004] The present invention provides an analgesic and anti-inflammatory gel and a preparation method thereof, which solves the problems of poor antibacterial effect and long healing time of the gel in the related art.

[0005] The technical solution of the present invention is as follows:

[0006] An analgesic and anti-inflammatory gel comprises the following components in parts by weight: 6-8 parts of traditional Chinese medicine additives, 0.5-1 parts of polyvinyl caprolactam, 2-4 parts of oxidized sodium alginate, 1-3 parts of maltooligosaccharides, 1-2 parts of histidine, 0.05-0.1 parts of silymarin, 3-6 parts of moisturizing agents, 0.2-0.4 parts of preservatives, and 75-85 parts of water.

[0007] As a further technical solution, the mass ratio of the oxidized sodium alginate to polyvinyl caprolactam is 1-2:0.5-1.

[0008] As a further technical solution, the mass ratio of the oxidized sodium alginate to polyvinyl caprolactam is 0.6:2.6.

[0009] The present invention finds that when the mass ratio of oxidized sodium alginate to polyvinyl caprolactam is 0.6:2.6, the antibacterial rate of the gel can be further improved and the wound healing time can be shortened.

[0010] As a further technical solution, the preparation method of the oxidized sodium alginate is:

[0011] S1, mixing and oxidizing a sodium alginate aqueous solution and a sodium periodate aqueous solution, and then adding ethylene glycol to obtain a mixture;

[0012] S2. Sodium chloride is added to the mixture and mixed, and then ethanol is added to precipitate to obtain oxidized sodium alginate.

[0013] As a further technical solution, the traditional Chinese medicine additive includes the following components in parts by weight: 10-15 parts of Andrographis paniculata, 5-10 parts of Datura stramonium, 2-5 parts of Zanthoxylum bungeanum, 5-10 parts of Aconiti Lateralis Preparata, 15-20 parts of Honeysuckle, 15-20 parts of Taraxacum mongolicum, 2-4 parts of Scutellaria baicalensis, and 12-16 parts of Portulaca oleracea.

[0014] As a further technical solution, the moisturizing agent includes one or more of glycerol, mannitol, and sorbitol.

[0015] As a further technical solution, the preservative includes one or more of chlorphenesin and phenoxyethanol.

[0016] The present invention also includes a method for preparing an analgesic and anti-inflammatory gel, comprising the following steps:

[0017] S1, mixing polyvinyl caprolactam, oxidized sodium alginate and water uniformly to obtain a mixed solution;

[0018] S2. Add the remaining components to the mixed solution and mix them evenly to obtain a gel.

[0019] As a further technical solution, the stirring speed of the mixing in S1 is 100-150 rpm, and the stirring time is 60-90 min.

[0020] As a further technical solution, the mixing speed in S2 is 150-200 rpm, and the mixing time is 30-60 min.

[0021] The working principle and beneficial effects of the present invention are:

[0022] 1. The gel of the present invention is added with Chinese medicinal additives, histidine and silymarin to improve the antibacterial, analgesic and anti-inflammatory effects of the gel. The strength of the gel network is improved by adding oxidized sodium alginate and polyvinyl caprolactam, and the adsorption effect of components such as Chinese medicinal additives and histidine is increased, thereby improving the antibacterial and anti-inflammatory effects and shortening the wound healing time.

[0023] 2. The analgesic and anti-inflammatory gel prepared by the present invention can be used both before and after orthognathic surgery. Using it before surgery can relieve the patient's nervousness about orthognathic surgery, improve the microbial environment of the oral cavity before surgery, and reduce the patient's gingival swelling and pain; using it after surgery can relieve the patient's pain caused by surgery, improve the microbial environment in the patient's oral cavity, accelerate the healing of postoperative wounds, and reduce the impact on the patient's quality of life after surgery. DETAILED DESCRIPTION

[0024] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The preparation methods of oxidized sodium alginate in the following examples and comparative examples are:

[0026] S1, mixing 5 parts of 2.5 wt% sodium alginate aqueous solution and 3 parts of 0.2 mol / L sodium periodate aqueous solution, oxidizing in the dark for 24 hours, and then adding 0.5 parts of ethylene glycol to obtain a mixture;

[0027] S2. Add 0.3 parts of sodium chloride to the mixture, mix, and then add 80 parts of ethanol to precipitate to obtain oxidized sodium alginate.

[0028] Example 1

[0029] The preparation method of the gel comprises the following steps:

[0030] S1, 0.5 parts of polyvinyl caprolactam, 2 parts of oxidized sodium alginate and 75 parts of water were stirred at a speed of 100 rpm for 90 minutes to obtain a mixed solution;

[0031] S2. Add 6 parts of traditional Chinese medicine additives, 1 part of maltooligosaccharide, 1 part of histidine, 0.05 parts of silymarin, 3 parts of moisturizer and 0.2 parts of preservative into the mixed solution, stir at a speed of 150 rpm for 60 minutes to obtain a gel.

[0032] Wherein, the preparation method of the drug additive is:

[0033] The preparation method is prepared by mixing 10 parts of Andrographis paniculata, 5 parts of Datura stramonium, 2 parts of Zanthoxylum bungeanum, 5 parts of Aconiti Lateralis Preparata, 15 parts of Honeysuckle, 15 parts of Taraxacum Herba, 2 parts of Scutellariae Baicalensis, and 12 parts of Portulaca Oleracea.

[0034] Example 2

[0035] The preparation method of the gel comprises the following steps:

[0036] S1, 0.6 parts of polyvinyl caprolactam, 2.5 parts of oxidized sodium alginate and 80 parts of water were stirred at a rotation speed of 130 rpm for 70 minutes to obtain a mixed solution;

[0037] S2. Add 7 parts of traditional Chinese medicine additives, 2 parts of maltooligosaccharides, 1.5 parts of histidine, 0.08 parts of silymarin, 4 parts of moisturizer, and 0.3 parts of preservative into the mixed solution, stir at a rotation speed of 160 rpm for 50 minutes to obtain a gel.

[0038] Wherein, the preparation method of the drug additive is:

[0039] The preparation method is prepared by mixing 13 parts of Andrographis paniculata, 6 parts of Datura stramonium, 3 parts of Zanthoxylum bungeanum, 8 parts of Aconiti Lateralis Preparata, 17 parts of Honeysuckle, 17 parts of Taraxacum Herba, 3 parts of Scutellariae Baicalensis, and 14 parts of Portulaca Oleracea.

[0040] Example 3

[0041] The preparation method of the gel comprises the following steps:

[0042] S1, stirring 0.7 parts of polyvinyl caprolactam, 3 parts of oxidized sodium alginate and 85 parts of water at a rotation speed of 130 rpm for 60 minutes to obtain a mixed solution;

[0043] S2. Add 8 parts of traditional Chinese medicine additives, 3 parts of maltooligosaccharides, 2 parts of histidine, 0.1 parts of silymarin, 6 parts of moisturizers, and 0.4 parts of preservatives to the mixed solution, stir at a rotation speed of 200 rpm for 30 minutes to obtain a gel.

[0044] Wherein, the preparation method of the drug additive is:

[0045] The preparation method is prepared by mixing 15 parts of Andrographis paniculata, 10 parts of Datura stramonium, 5 parts of Zanthoxylum bungeanum, 10 parts of Aconiti Lateralis Preparata, 20 parts of Honeysuckle, 20 parts of Taraxacum Herba, 4 parts of Scutellariae Baicalensis, and 16 parts of Portulaca Oleracea.

[0046] Example 4

[0047] Compared with Example 2, Example 4 is different in that 0.4 parts of polyvinyl caprolactam and 2.7 parts of oxidized sodium alginate are used.

[0048] Example 5

[0049] Compared with Example 2, Example 5 is different in that 0.7 parts of polyvinyl caprolactam and 2.4 parts of oxidized sodium alginate are used.

[0050] Comparative Example 1

[0051] Compared with Example 2, Comparative Example 1 does not add polyvinyl caprolactam, and the other parts are the same as Example 2.

[0052] Comparative Example 2

[0053] Compared with Example 2, in Comparative Example 2, polyvinyl caprolactam is replaced with an equal amount of ethylenediamine, and the rest is the same as Example 2.

[0054] Comparative Example 3

[0055] Compared with Example 2, in Comparative Example 3, oxidized sodium alginate is replaced with chitosan, and the rest is the same as Example 2.

[0056] Test example

[0057] (1) The antibacterial properties of the gels prepared in Examples 1-5 and Comparative Examples 1-3 were determined by the following method:

[0058] The antibacterial effect of the gel on the tested bacteria (Escherichia coli and Staphylococcus aureus) was determined by agar diffusion paper method and colony counting method. 8 CFU / mL of the test bacterial suspension is evenly spread on the surface of the solidified nutrient agar medium plate, 100μL per plate; under sterile conditions, the gel is spread on a sterilized and dried filter paper with a diameter of 0.3cm, and the prepared gel paper (with the sterilized filter paper as the blank control) is pasted on the plate coated with the test strain, and four paper pieces are placed on each plate, and cultured at 37℃ for 24h, and the contact surface culture is taken for gradient elution to prepare a diluted bacterial suspension, 1mL of the diluted bacterial suspension is taken into the plate, and the plate is poured with agar, mixed, and after solidification, it is inverted and cultured at 37℃ for 24h, and the total number of colonies is calculated. The calculation method of the total number of colonies refers to the method in GB47892-2010 "National Food Safety Standard for Determination of Total Number of Colonies in Food Microbiological Examination";

[0059] Inhibition rate (100%) = (total number of colonies in blank control - total number of colonies in gel) / total number of colonies in blank control × 100;

[0060] The measurement results are shown in Table 1.

[0061] Table 1 Antibacterial test results of the gels in Examples 1-5 and Comparative Examples 1-3

[0062]

[0063]

[0064] Compared with Example 2, Example 4-5 changed the mass ratio of polyvinyl caprolactam and oxidized sodium alginate. As a result, the antibacterial rates of Example 2 against Escherichia coli and Staphylococcus aureus were higher than those of Example 4-5, indicating that when the mass ratio of polyvinyl caprolactam and oxidized sodium alginate was 0.6:2.6, the antibacterial rate of the gel against Escherichia coli and Staphylococcus aureus could be further improved.

[0065] Compared with Example 2, Comparative Example 1 does not add polyvinyl caprolactam, Comparative Example 2 replaces polyvinyl caprolactam with an equal amount of ethylenediamine, and Comparative Example 3 replaces oxidized sodium alginate with chitosan. As a result, the antibacterial rates of Example 2 against Escherichia coli and Staphylococcus aureus are higher than those of Comparative Examples 1-3, indicating that the simultaneous addition of polyvinyl caprolactam and oxidized sodium alginate can increase the antibacterial rates of the gel against Escherichia coli and Staphylococcus aureus.

[0066] (2) Animal experiments

[0067] 48 male New Zealand rabbits, each weighing 2-3 kg, were randomly divided into 8 groups, 6 rabbits in each group. Each rabbit was subjected to 30% TBSA shallow II degree burns according to the standard burn model preparation method for clinical animal experiments, and the gels prepared in Examples 1-5 and Comparative Examples 1-3 were used as dressings, respectively, and the dressings were changed twice a day until the wound healed. The animal experiment was terminated, and the healing time was calculated. The experimental results are shown in Table 2.

[0068] Table 2 Wound healing time experimental results

[0069]

[0070] Compared with Example 2, Example 4-5 changed the mass ratio of polyvinyl caprolactam and sodium alginate oxide. As a result, the healing time of Example 2 was shorter than that of Example 4-5, indicating that the healing time can be further shortened when the mass ratio of polyvinyl caprolactam and sodium alginate oxide is 0.6:2.6.

[0071] Compared with Example 2, Comparative Example 1 does not add polyvinyl caprolactam, Comparative Example 2 replaces polyvinyl caprolactam with an equal amount of ethylenediamine, and Comparative Example 3 replaces oxidized sodium alginate with chitosan. As a result, the healing time of Example 2 is shorter than that of Comparative Examples 1-3, indicating that the simultaneous addition of polyvinyl caprolactam and oxidized sodium alginate can shorten the wound healing time.

[0072] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An analgesic and anti-inflammatory gel, characterized in that: The invention is prepared from the following components in parts by weight: 6-8 parts of traditional Chinese medicine additives, 0.5-1 parts of polyvinyl caprolactam, 2-4 parts of oxidized sodium alginate, 1-3 parts of oligomaltose, 1-2 parts of histidine, 0.05-0.1 parts of silymarin, 3-6 parts of moisturizing agent, 0.2-0.4 parts of preservative, and 75-85 parts of water; The traditional Chinese medicine additive consists of the following components in parts by weight: 10-15 parts of Andrographis paniculata, 5-10 parts of Datura stramonium, 2-5 parts of Zanthoxylum bungeanum, 5-10 parts of Aconiti Lateralis Preparata, 15-20 parts of Honeysuckle, 15-20 parts of Taraxacum mongolicum, 2-4 parts of Scutellaria baicalensis, and 12-16 parts of Portulaca oleracea.

2. The analgesic and anti-inflammatory gel according to claim 1, characterized in that: The mass ratio of the oxidized sodium alginate to polyvinyl caprolactam is 1-2:0.5-1.

3. The analgesic and anti-inflammatory gel according to claim 1, characterized in that: The preparation method of the oxidized sodium alginate is: S1, mixing and oxidizing a sodium alginate aqueous solution and a sodium periodate aqueous solution, and then adding ethylene glycol to obtain a mixture; S2. Sodium chloride is added to the mixture and mixed, and then ethanol is added to precipitate to obtain oxidized sodium alginate.

4. The analgesic and anti-inflammatory gel according to claim 1, characterized in that: The humectant is selected from one or more of glycerol, mannitol and sorbitol.

5. The method for preparing an analgesic and anti-inflammatory gel according to claim 1, characterized in that: The preservative is selected from one or more of chlorphenesin and phenoxyethanol.

6. The method for preparing an analgesic and anti-inflammatory gel according to claim 1, characterized in that: The following steps are involved: S1, stirring and mixing polyvinyl caprolactam, oxidized sodium alginate and water to obtain a mixed solution; S2. Add the remaining components to the mixed solution and stir to mix evenly to obtain a gel.

7. The method for preparing an analgesic and anti-inflammatory gel according to claim 6, characterized in that: The stirring speed of the mixing in S1 is 100-150 rpm, and the stirring time is 60-90 min.

8. The method for preparing an analgesic and anti-inflammatory gel according to claim 6, characterized in that: The mixing speed in S2 is 150-200 rpm, and the mixing time is 30-60 min.

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