A fishery regulating composition with lipid-lowering and blood sugar-controlling effects, and its preparation method and application

Abstract

The present invention belongs to the technical field of aquaculture, and discloses a fishery regulating composition with lipid-lowering and blood sugar-controlling effects, and a preparation method and application thereof. The composition comprises the following components in parts by weight: 2 to 50 parts of berberine, 4 to 50 parts of allicin, 2 to 30 parts of benfotiamine, 2 to 50 parts of γ-aminobutyric acid, 4 to 20 parts of sodium oxalate, and 200 to 530 parts of γ-glutamyl-phenylalanine. Among them, γ-glutamyl-phenylalanine is obtained by fermenting a mixture of cotton stalks and corn stalks. The fishery regulating composition of the present invention makes full use of the different biological structures of the raw materials to carry out scientific compatibility and exert synergistic effects. By regulating the sugar and lipid metabolism function of farmed animals, the effect of lipid-lowering and blood sugar-controlling is achieved, thereby improving the sugar metabolism function of aquatic animals and significantly improving their utilization rate of carbohydrates in feed. The regulating composition is used in freshwater fish farming.

Description

Technical Field

[0001] The present invention belongs to the technical field of aquaculture, and more specifically, relates to a fishery regulating and controlling composition having the effects of lowering blood lipids and controlling blood sugar, and a preparation method and application thereof. Background Art

[0002] As the cheapest energy source among the three major nutrients, adding an appropriate amount of sugar to aquatic feed can save the amount of protein raw materials in the feed. However, fish are congenitally diabetic and have a low utilization rate of sugars. After eating high-carbohydrate feeds, they will have persistent hyperglycemia symptoms. At the same time, there are also fatty liver phenomena such as significantly increased blood lipid and liver fat levels, hypertrophy of the liver, pale liver color, and increased vacuoles in hepatocytes. These phenomena can cause nutritional metabolic diseases, which in turn lead to decreased growth or even death of fish. At present, there are few reports on the development of aquatic animal sugar metabolism regulators, and existing studies have mainly focused on the hypoglycemic effect of Western medicine. However, Western medicines are prone to drug resistance and drug residues in aquatic animals during use, which restricts their widespread use in feed. At the same time, the bioavailability of aquatic animal sugar metabolism regulators currently sold on the market is low, which greatly limits their function.

[0003] In recent years, with the general trend of reducing, replacing and eliminating antibiotics in the national aquaculture industry, the research on active ingredients of medicinal plants, high-efficiency vitamins and amino acid products has received increasing attention. Berberine, also known as berberine, is a quaternary ammonium alkaloid in the traditional Chinese medicine Coptis chinensis; allicin is an organic sulfur compound extracted from the bulb (garlic head) of the Allium genus of the Allium family; benfotiamine is a derivative of thiamine (vitamin B1) and has higher bioavailability due to its fat solubility; γ-aminobutyric acid is a naturally occurring non-protein amino acid, which is considered to be an important inhibitory neurotransmitter in the central nervous system of organisms; sodium oxalate is an inhibitor of lactate dehydrogenase a in the body's glycolysis process. These substances have all been proven to be able to regulate the body's glucose metabolism function, but their mechanisms of action are significantly different. For example, γ-aminobutyric acid enhances the glucose metabolism function of brain cells by regulating the tricarboxylic acid cycle of brain tissue and increasing the activity of glucose phosphatase in the process of glucose metabolism. Benfotiamine regulates the glucose metabolism function of brain tissue by activating the glucose metabolism signaling pathway mediated by glycogen synthase kinase-3. Sodium oxalate, however, inhibits the activity of lactate dehydrogenase a, reduces the production of endogenous lactic acid, and thus regulates the sugar metabolism function of body tissues. In view of this, if the different characteristics of the biological structures and action mechanisms of the above five substances are used to scientifically match and make them play a synergistic role, and then a fishery sugar metabolism regulating composition with lipid-lowering and blood sugar-controlling functions is developed, it will be of great significance to reduce feed costs and improve the economic benefits of aquaculture.

[0004] The absorption rate and bioavailability of the regulated composition directly affect its effect, and increasing the appetitiveness of the regulated composition is an important method to improve its absorption rate and bioavailability. γ-Glutamyl-phenylalanine is a complex amino acid produced by microbial fermentation. It can significantly enhance the umami taste of food (such as soy sauce and model chicken soup) and has strong appetitiveness. In addition, microencapsulation technology refers to the process of encapsulating bioactive substances (capsule core) into tiny capsules using natural or synthetic polymer materials (capsule materials). This technology can improve the stability of bioactive substances and enhance their bioavailability. Summary of the invention

[0005] In order to solve the above-mentioned deficiencies and shortcomings of the prior art, the object of the present invention is to provide a fishery regulating composition with the effect of lowering blood lipids and controlling blood sugar.

[0006] Another object of the present invention is to provide a method for preparing the above-mentioned fishery regulating and controlling composition with lipid-lowering and blood sugar-controlling effects.

[0007] Another object of the present invention is to provide the application of the above-mentioned fishery regulating composition with the effects of lowering blood lipids and controlling blood sugar.

[0008] The purpose of the present invention is achieved through the following technical solutions:

[0009] A fishery regulating and controlling composition with lipid-lowering and blood sugar-control properties, the composition comprising the following components in parts by weight: 2-50 parts of berberine; 4-50 parts of allicin; 2-30 parts of benfotiamine; 2-50 parts of gamma-aminobutyric acid; 4-30 parts of sodium oxalate; 200-530 parts of gamma-glutamyl-phenylalanine;

[0010] Among them, γ-glutamyl-phenylalanine is prepared by washing cotton stalks and corn stalks with distilled water, drying them, and then crushing them into a mixture of 80-120 meshes. The mixture is added with Mandel salt solution and then inoculated with a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens. The pH of the mixed solution is adjusted to 8-10, and the mixture is cultured at a constant temperature of 35-50° C. for 2-4 days. The obtained enzyme fermentation solution is centrifuged at 1000-1300 r / min for 15-30 minutes, and the supernatant is rotary evaporated at 105-150° C. to obtain the product.

[0011] Preferably, the composition comprises the following components in parts by weight: 10 to 45 parts of berberine; 15 to 40 parts of allicin; 8 to 25 parts of benfotiamine; 10 to 43 parts of γ-aminobutyric acid; 7 to 25 parts of sodium oxalate; and 260 to 500 parts of γ-glutamyl-phenylalanine.

[0012] Further preferably, the composition comprises the following components in parts by weight: 20-40 parts of berberine; 17-35 parts of allicin; 10-20 parts of benfotiamine; 15-40 parts of γ-aminobutyric acid; 10-20 parts of sodium oxalate; and 300-470 parts of γ-glutamyl-phenylalanine.

[0013] More preferably, the composition comprises the following components in parts by weight: 23-38 parts of berberine; 20-33 parts of allicin; 13-18 parts of benfotiamine; 30-39 parts of γ-aminobutyric acid; 12-16 parts of sodium oxalate; and 350-450 parts of γ-glutamyl-phenylalanine.

[0014] The preparation method of the fishery regulating and controlling composition having the functions of lowering blood lipids and controlling blood sugar comprises the following specific steps:

[0015] S1. Wash cotton stalks and corn stalks with distilled water, dry them in the sun, and then crush them into a mixture of 80-120 mesh. Add Mandel salt solution to the mixture and then inoculate it into a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens. Adjust the pH of the mixture to 8-10, culture it at a constant temperature of 35-50° C. for 2-4 days, centrifuge the obtained enzyme fermentation liquid at 1000-1300 r / min for 15-30 min, and take the supernatant and rotary evaporate it at 105-150° C. to obtain γ-glutamyl-phenylalanine;

[0016] S2. Grind berberine, allicin, benfotiamine, γ-aminobutyric acid, sodium oxalate and γ-glutamyl-phenylalanine separately, pass through a 120-150 mesh sieve and mix thoroughly;

[0017] S3. Add β-cyclodextrin and dispersant to methanol aqueous solution and homogenize at a speed of 10000-12000 r / min for 10-15 min, add 10-15% methanol solution of the regulating composition under stirring conditions of 1000-1300 r / min, stir at 30-65°C, 1000-1300 r / min for 4-5 hours, naturally cool to room temperature and stand for 24-36 hours, filter and wash, cool and dry to obtain a fishery regulating composition with the effects of lowering blood lipids and controlling blood sugar.

[0018] Preferably, the mass ratio of cotton stalks to corn stalks in step S1 is (1-2.5):(3-5.4), the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens in the mixed bacterial solution is (2.5-3):(1-2.5); the volume ratio of the mass of the mixture to the Mandel salt solution is (1-2) g:(2-5) mL.

[0019] Preferably, the temperature of the berberine, allicin, benfotiamine, γ-aminobutyric acid, sodium oxalate and γ-glutamyl-phenylalanine during pulverization in step S2 is less than 40°C.

[0020] Preferably, the dispersant in step S3 is a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 in a weight ratio of 2:1, the methanol aqueous solution is a mixed solution of methanol and distilled water in a volume ratio of 1:3; the mass ratio of the β-cyclodextrin and the dispersant is 3:(3.5-5); the volume ratio of the total mass of the β-cyclodextrin and the dispersant to the methanol aqueous solution is (400-500) g:(1-2.5) L; the volume ratio of the methanol aqueous solution to the methanol solution of the regulating composition is 20:(1-1.5).

[0021] The application of the fishery regulating and controlling composition with lipid-lowering and blood sugar-controlling effects in freshwater fish farming.

[0022] Preferably, the addition amount of the regulating composition in the freshwater fish feed is 0.5-1.4wt%; the freshwater fish is grass carp, bighead bream, carp, crucian carp, tilapia, channel catfish, largemouth bass or snakehead.

[0023] The present invention mixes cotton stalks and corn stalks in proportion and then adds a composite strain for fermentation to obtain high-purity γ-glutamyl-phenylalanine, which is then used to prepare a fishery control composition. This process can greatly improve the comprehensive utilization of crop straw resources, which is of great significance for promoting farmers' income increase, environmental protection, resource conservation and sustainable development of agricultural economy. At the same time, in order to further improve the bioavailability and effect of the composition, microencapsulation technology is applied to the preparation process of the composition. After technical optimization and breeding experiment screening, a fishery control composition with strong attractant is obtained based on the pharmacological synergistic relationship of benfotiamine, berberine, allicin, γ-aminobutyric acid and sodium oxalate. As an efficient and environmentally friendly aquatic feed additive, it can greatly reduce the use of protein and fat raw materials in feed and significantly improve the economic benefits of aquaculture.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The control composition of the present invention uses berberine, allicin, benfotiamine, γ-aminobutyric acid and sodium oxalate as main raw materials, and makes full use of the different characteristics of their biological structures to carry out scientific compatibility in order to exert synergistic effects. At the same time, plant straw resources are fully utilized, and strong food γ-glutamyl-phenylalanine is produced by biological fermentation technology, and added to the control composition. In addition, microencapsulation technology is also applied to the processing of the control composition to improve its bioavailability to aquatic animals.

[0026] 2. The regulating composition of the present invention achieves the effect of lowering blood lipids and controlling blood sugar by regulating the glucose and lipid metabolism function of aquatic animals, thereby improving the glucose metabolism function of aquatic animals and increasing their utilization rate of carbohydrates in feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The effect of different feeds on the weight gain rate of young bream in Application Example 1;

[0028] Figure 2 The effect of different feeds on the weight gain rate of young bream in Application Example 2;

[0029] Figure 3 The effect of different feeds on liver fat content of young bream in Application Example 2;

[0030] Figure 4 The effect of different feeds on blood sugar levels of young bream in Application Example 2;

[0031] Figure 5 It is the effect of different feeds on the blood sugar level of carp in Application Example 4;

[0032] Figure 6 The effect of different feeds on liver fat content of juvenile largemouth bass in Application Example 7;

[0033] Figure 7 The effect of different feeds on the blood sugar clearance ability of Channa argus in Application Example 9;

[0034] Figure 8 This is the dynamic change curve of blood sugar of snakehead fish in application example 9 with different feeds. DETAILED DESCRIPTION

[0035] The content of the present invention is further described below in conjunction with specific examples, but it should not be construed as limiting the present invention. If not specifically indicated, the technical means used in the examples are conventional means well known to those skilled in the art. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0036] In the embodiment of the present invention, the Mandel salt solution includes a major element solution and a trace element solution, wherein the major element solution is prepared by dissolving 2.0 g of K2HPO4, 1.4 g of (NH4)2SO4, 0.3 g of MgSO4·7H2O, and 0.3 g of CaCl2 in 600 mL of double distilled water, and the trace element solution is prepared by dissolving 5.0 mg of FeSO4·7H2O, 1.6 mg of MnSO4·H2O, 1.4 mg of ZnSO4·7H2O, and 2.0 mg of CoCl2 in 200 mL of double distilled water. After mixing the major element solution and the trace element solution, the pH is adjusted to 5.5-6.0 with HCl, and then the volume is fixed to 1000 mL with double distilled water to obtain the Mandel salt solution.

[0037] Example 1

[0038] 1. Wash the cotton stalks and corn stalks with distilled water, dry them, and crush them to 100 mesh. Take 10g of the mixture (the mass ratio of cotton stalks to corn stalks is 1:3) and add 20mL of Mandel salt solution. Then, inoculate the mixed bacterial liquid of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens is 2.5:1), adjust the pH to 8.5, and culture in a constant temperature incubator at 45°C for 2 days. The obtained enzyme fermentation liquid is centrifuged at 1300r / min for 20min, and the supernatant is rotary evaporated at 105°C to obtain γ-glutamyl-phenylalanine.

[0039] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 2g of berberine, 4g of allicin, 2g of benfotiamine, 2g of sodium oxalate, 4g of γ-aminobutyric acid and 11g of γ-glutamyl-phenylalanine, which have been sieved through a 120-mesh sieve, are fully mixed. This process requires that the mixture be uniform and stable to obtain 25g of a roughly controlled composition.

[0040] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 10000r / min for 10min. Add 100mL of a methanol solution of a 10wt% control composition under stirring conditions of 1300r / min, stir at 50°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, cool and dry, and obtain 10g of a control composition for fishery.

[0041] Example 2

[0042] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 13g of the mixture (the mass ratio of cotton stalks to corn stalks is 2.5:3) and add 50mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens is 3:2), adjust the pH to 10.0, and culture in a 50°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1000r / min for 30min, and the supernatant is rotary evaporated at 150°C to obtain γ-glutamyl-phenylalanine.

[0043] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 3g of berberine, 3g of allicin, 4g of benfotiamine, 3g of sodium oxalate, 2g of γ-aminobutyric acid and 10g of γ-glutamyl-phenylalanine that have passed through a 120-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25g of a roughly controlled composition.

[0044] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 12000r / min for 10min. Add 100mL of a methanol solution of a 10wt% control composition under stirring conditions of 1200r / min, stir at 65°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0045] Example 3

[0046] 1. Wash the cotton stalks and corn stalks with distilled water, dry them, and crush them to 80 mesh. Take 20g of the mixture (the mass ratio of cotton stalks to corn stalks is 2:5) and add 43mL of Mandel salt solution. Then, inoculate the mixed bacterial liquid of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae and Bacillus amyloliquefaciens = 3:1.5), adjust the pH to 9.0, and culture at 40°C for 4 days. Finally, the enzyme fermentation liquid is centrifuged at 1200r / min for 25min, and the supernatant is rotary evaporated at 145°C to obtain γ-glutamyl-phenylalanine.

[0047] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 1 g of berberine, 4 g of allicin, 3 g of benfotiamine, 6 g of sodium oxalate, 1 g of γ-aminobutyric acid and 10 g of γ-glutamyl-phenylalanine after passing through a 120-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25 g of a roughly controlled composition.

[0048] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 12000r / min for 10min. Add 100mL of a methanol solution of the control composition with a mass concentration of 10wt% under stirring conditions of 1300r / min, stir at 60°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0049] Example 4

[0050] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 17g of the mixture (the mass ratio of cotton stalks to corn stalks is 1.5:3.5) and add 40mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens = 2.3:2), adjust the pH to 8.0, and culture in a 45°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1200r / min for 30min, and the supernatant is rotary evaporated at 150°C to obtain γ-glutamyl-phenylalanine.

[0051] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 5 g of berberine, 1 g of allicin, 1 g of benfotiamine, 2 g of sodium oxalate, 7 g of γ-aminobutyric acid and 9 g of γ-glutamyl-phenylalanine that have passed through a 120-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25 g of a roughly controlled composition.

[0052] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 11000r / min for 10min. Add 100mL of a methanol solution of a 10wt% control composition under stirring conditions of 1300r / min, stir at 60°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0053] Example 5

[0054] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 15g of the mixture (the mass ratio of cotton stalks to corn stalks is 2.5:4.0) and add 40mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens = 2.5:1), adjust the pH to 9.5, and culture in a 50°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1000r / min for 30min, and the supernatant is rotary evaporated at 140°C to obtain γ-glutamyl-phenylalanine.

[0055] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 1 g of berberine, 1 g of allicin, 4 g of benfotiamine, 4 g of sodium oxalate, 1 g of γ-aminobutyric acid and 14 g of γ-glutamyl-phenylalanine after passing through a 120-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25 g of a roughly controlled composition.

[0056] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 11000r / min for 10min. Add 100mL of a methanol solution of a 10wt% control composition under stirring conditions of 1300r / min, stir at 55°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0057] Example 6

[0058] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 15g of the mixture (the mass ratio of cotton stalks to corn stalks is 2.5:4.5) and add 50mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae and Bacillus amyloliquefaciens = 2.5:2), adjust the pH to 10.0, and culture in a 40°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1300r / min for 30min, and the supernatant is rotary evaporated at 140°C to obtain γ-glutamyl-phenylalanine.

[0059] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 4 g of berberine, 8 g of allicin, 1 g of benfotiamine, 1 g of sodium oxalate, 3 g of γ-aminobutyric acid and 8 g of γ-glutamyl-phenylalanine that have passed through a 120-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25 g of a roughly controlled composition.

[0060] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 12000r / min for 10min. Add 100mL of a methanol solution of the control composition with a mass concentration of 10wt% under stirring conditions of 1300r / min, stir at 60°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0061] Example 7

[0062] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 20g of the mixture (the mass ratio of cotton stalks to corn stalks is 2.5:5) and add 30mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens = 2:1), adjust the pH to 10.0, and culture in a 35°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1300r / min for 30min, and the supernatant is rotary evaporated at 150°C to obtain γ-glutamyl-phenylalanine.

[0063] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40°C when crushed. According to the step-by-step mixing method, 2g of berberine, 1g of allicin, 2g of benfotiamine, 1g of sodium oxalate, 3g of γ-aminobutyric acid and 16g of γ-glutamyl-phenylalanine after passing through a 150-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25g of a roughly adjusted composition.

[0064] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 11000r / min for 10min. Add 100mL of a methanol solution of the control composition with a mass concentration of 10wt% under stirring conditions of 1300r / min, stir at 62°C for 4.5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, cool and dry to obtain 10g of the control composition.

[0065] Example 8

[0066] 1. Wash the cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 20g of the mixture (the mass ratio of cotton stalks to corn stalks is 1.5:3) and add 50mL of Mandel salt solution. Then, inoculate the mixed bacterial liquid of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae and Bacillus amyloliquefaciens = 2.6:1.5), adjust the pH to 10.0, and culture in a constant temperature incubator at 50°C for 4 days. Finally, the enzyme fermentation liquid is centrifuged at 1000r / min for 25min, and the supernatant is rotary evaporated at 150°C to obtain γ-glutamyl-phenylalanine.

[0067] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40°C when crushed. 3g of berberine, 3g of allicin, 4g of benfotiamine, 4g of sodium oxalate, 1g of γ-aminobutyric acid and 10g of γ-glutamyl-phenylalanine after passing through a 150-mesh sieve are fully mixed according to a step-by-step mixing method. This process requires that the mixture be uniform and stable to obtain 25g of a roughly controlled composition.

[0068] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 12000r / min for 10min. Add 100mL of a methanol solution of a 10wt% control composition under stirring conditions of 1300r / min, stir at 62°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0069] Example 9

[0070] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 meshes. Take 10g of the mixture (the mass ratio of cotton stalks to corn stalks is 2.5-5.0) and add 50mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens = 2.5:1.0), adjust the pH to 10.0, and culture in a 50°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1200r / min for 30min, and the supernatant is rotary evaporated at 150°C to obtain γ-glutamyl-phenylalanine.

[0071] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40°C when crushed. 3g of berberine, 3g of allicin, 2g of benfotiamine, 2g of sodium oxalate, 8g of γ-aminobutyric acid and 7g of γ-glutamyl-phenylalanine after passing through a 130-mesh sieve are fully mixed according to a step-by-step mixing method. This process requires that the mixture be uniform and stable to obtain 25g of a roughly controlled composition.

[0072] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2.5L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 12000r / min for 10min. Add 100mL of a methanol solution of a 10wt% control composition under stirring conditions of 1300r / min, stir at 60°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, cool and dry to obtain 10g of the control composition.

[0073] Example 10

[0074] 1. Wash cotton stalks and corn stalks with distilled water, dry them, and crush them to 120 mesh. Take 20g of the mixture (the mass ratio of cotton stalks to corn stalks is 2.5:3) and add 0mL of Mandel salt solution. Then, inoculate a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens (the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens = 2.8:1.5), adjust the pH to 10.0, and culture in a 50°C constant temperature incubator for 4 days. The resulting enzyme fermentation liquid is centrifuged at 1300r / min for 30min, and the supernatant is rotary evaporated at 150°C to obtain γ-glutamyl-phenylalanine.

[0075] 2. Berberine, allicin, benfotiamine, sodium oxalate, γ-aminobutyric acid and γ-glutamyl-phenylalanine must be less than 40° C. According to the step-by-step mixing method, 1 g of berberine, 3 g of allicin, 2 g of benfotiamine, 2 g of sodium oxalate, 4 g of γ-aminobutyric acid and 13 g of γ-glutamyl-phenylalanine after passing through a 150-mesh sieve are fully mixed. This process requires that the mixture be uniform and stable to obtain 25 g of a roughly controlled composition.

[0076] 3. Add 300g of β-cyclodextrin and 500g of dispersant (a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 with a weight ratio of 2:1) to 2L of methanol aqueous solution (a mixed solution of methanol and distilled water with a volume ratio of 1:3) and homogenize at a speed of 12000r / min for 10min. Add 100mL of a methanol solution of the control composition with a mass concentration of 10wt% under stirring conditions of 1300r / min, stir at 65°C for 5h, cool naturally to room temperature and stand for 24h. Then filter and wash with a Buchner funnel, and cool and dry to obtain 10g of the control composition.

[0077] Application Example 1

[0078] Breeding experiment and result analysis of young giant bream (about 140g): This breeding experiment was carried out at the Aquatic Teaching and Research Base of Nanjing Agricultural University (Xingdian Town, Pukou District, Nanjing). The young giant bream used was purchased from the National Aquatic Breeding Farm in Yangzhou City, Jiangsu Province. 360 young fish of uniform specifications (initial weight: 143.12±2.10g) were selected and randomly placed in 12 cages (specifications: 2.0m×1.0m×1.0m), with 30 fish in each cage. Among them, (1) the control group was fed with a basic feed (the sugar level of the feed was 35%); (2) the high-sugar group was fed with a high-sugar feed (the sugar level of the feed was 45%); (3) the high-sugar + regulation composition group was fed with a high-sugar feed + 1.0% of the regulation composition prepared in Example 1. The feed formula components are shown in Table 1. The culture was continued for 12 weeks, during which the feeding and growth of young Megalobrama amblycephala were observed. After the culture was completed, blood and tissue samples were collected for subsequent index analysis. The specific results are shown in Table 2.

[0079] Table 1 Test feed formula

[0080]

[0081]

[0082] Table 2 Effects of different feeds on growth and blood biochemical parameters of young Megalobrama amblycephala

[0083]

[0084] Note: The same lowercase letters in the same row indicate no significant difference.

[0085] Figure 1 This is the effect of different feeds on the weight gain rate of young bream in Application Example 1. The same lowercase letters in the figure indicate that the difference is not significant. Figure 1 As shown in Table 2, the weight gain rate, pancreatic β-cell function and insulin sensitivity index of fish fed the high-sugar group were significantly lower than those of the control group (P<0.05), but the liver-to-body ratio, abdominal fat rate, liver glycogen and fat content, blood glucose level, insulin content, aspartate aminotransferase activity, alanine aminotransferase activity, plasma proinflammatory cytokine (tumor necrosis factor, interleukin-1β and interleukin-6) levels, liver proinflammatory cytokine (interleukin-1β and interleukin-6) levels and insulin resistance index were significantly higher than those of the control group (P<0.05). The high-sugar + regulation combination feed increased the fish's weight gain rate, pancreatic β-cell function and insulin sensitivity, but the liver-to-body ratio, abdominal fat rate, liver glycogen content, blood glucose level, aspartate aminotransferase activity, alanine aminotransferase activity, plasma proinflammatory cytokine (tumor necrosis factor, interleukin-1β and interleukin-6) levels, liver proinflammatory cytokine (interleukin-1β and interleukin-6) levels and insulin resistance index were significantly lower (P<0.05) than those in the high-sugar group.

[0086] The above results show that the feed containing the regulatory composition prepared in Example 1 can improve the excessive accumulation of liver fat and liver inflammation caused by the intake of high-sugar feed in the bighead carp, enhance the function of pancreatic β cells in the fish, promote the secretion and release of insulin, increase insulin sensitivity and reduce the degree of insulin resistance, and ultimately reduce blood sugar levels and improve the utilization rate of sugars in the feed by the bighead carp. In addition, the use of sugar metabolism regulators significantly reduces liver glycogen deposition and prevents the occurrence of liver glucose and lipid metabolism disorders caused by liver enlargement.

[0087] Application Example 2

[0088] The aquaculture experiment and result analysis of young bream (about 50g) was carried out in the indoor recirculating aquaculture system of the Animal Science Building of South China Agricultural University. The young bream were purchased from an aquatic seed company in Guangzhou. 960 young breams of uniform size (initial weight: 53.31±3.03g) were selected and randomly placed in 32 cages (specifications: 2.0m×1.0m×1.0m), with 30 fish in each cage. Among them, (1) HC, high sugar group, fed with high sugar feed (sugar level of feed was 45%); (2) HC1, high sugar + 1.0% berberine group; (3) HC2, high sugar + 1.0% allicin group; (4) HC3, high sugar + 1.0% benfotiamine group; (5) HC4, high sugar + 1.0% sodium oxalate group; (6) HC5, high sugar + 1.0% γ-aminobutyric acid group; (7) HC6, high sugar + 1.0% γ-glutamyl-phenylalanine; and (8) HC7, high sugar + regulatory composition group, fed with high sugar feed + 1.0% regulatory composition prepared in Example 1. The basic components of the feed formula are shown in Table 1. The feeding and growth conditions of the young giant bream were observed during the 12 weeks of breeding. After the breeding, blood and tissue samples were collected for subsequent index analysis. The specific results are shown in Table 1. Figure 2-4 shown.

[0089] Figure 2 The effect of different feeds on the weight gain rate of young bream in Application Example 2; Figure 3 The effect of different feeds on liver fat content of young bream in Application Example 2; Figure 4 This is the effect of different feeds on blood sugar levels of young bream in Application Example 2. The same lowercase letters in the figure indicate no significant difference. Figure 2-4 It can be seen that the weight gain rate of fish in the high-sugar group was significantly lower than that of fish in other test groups (P<0.05), but the change trends of their liver fat content and blood sugar levels were opposite (P<0.05). In the high-sugar feed additive group, the feed of high sugar + regulatory composition significantly increased the weight gain rate of fish, but the liver fat content and blood sugar level were significantly lower (P<0.05) than those of fish in each high-sugar feed additive group. The above results show that compared with each single regulatory substance, the regulatory composition is more helpful in promoting the growth of fish fed high-sugar feed, and has a significant lipid-lowering and blood sugar-controlling effect.

[0090] Application Example 3

[0091] Grass carp breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Department of Special Economic Animals and Aquatic Products of Nanjing Agricultural University. Grass carps with an average weight of about 42g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating combination group. Each group had 4 circulating breeding tanks (specification: 480L), 30 fish in each tank, and the breeding cycle was 12 weeks. The feed formula is shown in Table 1. After the breeding experiment, blood and liver samples were collected for index determination.

[0092] As shown in Table 3, after 12 weeks of breeding experiment, the weight gain rate, blood glucose clearance ability and insulin sensitivity index of the high-sugar group were significantly lower than those of the control group (P<0.05), while the liver-to-body ratio, liver glycogen content, liver fat content, blood glucose level, plasma proinflammatory cytokine (tumor necrosis factor, interleukin-1β and interleukin-6) levels, blood glucose half-life and liver proinflammatory cytokine (tumor necrosis factor and interleukin-1β) expression levels of the high-sugar group were significantly increased (P<0.05), indicating that long-term feeding of high-sugar feed can lead to liver fat accumulation in fish and produce liver inflammatory response, reduce tissue insulin sensitivity, and thus reduce blood glucose regulation ability and weight gain rate. However, the liver glycogen content, liver fat content, blood sugar level, plasma proinflammatory cytokine (tumor necrosis factor, interleukin 1β and interleukin 6) level, blood sugar half-life and liver proinflammatory cytokine expression of the fish in the high sugar + regulation composition group were significantly lower than those in the high sugar group (P<0.05), but the weight gain rate and plasma insulin level increased. This shows that the feed containing the regulation composition prepared in Example 1 can improve the liver fat accumulation and inflammatory response caused by the intake of high-sugar feed in grass carp, thereby enhancing the fish's blood sugar clearance ability, reducing blood sugar half-life, promoting insulin secretion, and ultimately improving the weight gain rate and the economic benefits of breeding.

[0093] Table 3 Effects of different feeds on growth performance and blood biochemical indicators of grass carp

[0094]

[0095] Note: The same lowercase letters in the same row indicate no significant difference.

[0096] Application Example 4

[0097] Carp breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Animal Science Building of South China Agricultural University. Carps with an average weight of about 51g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating composition group. Each group had 4 circulating breeding tanks (specification: 500L), 30 fish in each tank, and the breeding cycle was 12 weeks. The feed formula was the same as shown in Table 1, but 1.0% of the regulating composition of Example 2 was used. After the breeding experiment, blood and liver samples were collected for index determination.

[0098] Figure 5 The effects of different feeds on the blood glucose level of carp in Application Example 4 are shown in Table 4 and Table 4. Figure 5As shown, after 12 weeks of breeding experiments, the weight gain rate, blood sugar clearance ability and insulin sensitivity index of the high sugar group fish were significantly lower than those of the control group (P<0.05), while the abdominal fat rate, liver fat content, blood sugar level and blood sugar half-life of the high sugar group fish were significantly increased (P<0.05), indicating that long-term feeding of high sugar feed can lead to liver fat accumulation in fish, reduce tissue insulin sensitivity, and thus reduce blood sugar regulation ability, and the weight gain rate is significantly decreased. However, the liver fat content, blood sugar level and blood sugar half-life of the high sugar + regulation composition group fish were significantly lower than those of the high sugar group (P<0.05), but the weight gain rate and plasma insulin level were increased. This shows that the feed containing the regulation composition prepared in Example 2 can improve the liver fat accumulation caused by carp ingesting high sugar feed, enhance the blood sugar clearance ability of fish and promote insulin secretion, which is beneficial to improve the weight gain rate of fish.

[0099] Table 4 Effects of different feeds on growth performance and biochemical indicators of carp

[0100]

[0101] Note: The same lowercase letters in the same row indicate no significant difference.

[0102] Application Example 5

[0103] Crucian carp breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Animal Science Building of South China Agricultural University. Crucian carps with an average weight of about 10g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating composition group. Each group has 4 circulating breeding tanks (specification: 500L), 30 fish in each tank, and the breeding cycle is 12 weeks. The feed formula is the same as shown in Table 1, but 1.0% of the regulating composition of Example 5 is used. After the breeding experiment, blood and liver samples were collected for index determination.

[0104] As shown in Table 5, after 12 weeks of breeding experiment, the weight gain rate, blood sugar clearance ability and insulin sensitivity index of the high sugar group fish were significantly lower than those of the control group (P<0.05), while the abdominal fat rate, liver fat content, blood sugar level and blood sugar half-life of the high sugar group fish were significantly increased (P<0.05), indicating that the fish fed with high sugar feed had liver fat accumulation, tissue insulin sensitivity decreased, thereby reducing blood sugar regulation ability, and weight gain rate decreased significantly. However, the liver fat content, blood sugar level and blood sugar half-life of the high sugar + regulation composition group fish were significantly lower than those of the high sugar group (P<0.05), but the weight gain rate and plasma insulin level increased. This shows that the feed containing the regulation composition prepared in Example 5 can improve the liver fat accumulation caused by crucian carp due to eating high sugar feed, enhance the blood sugar clearance ability of fish and promote insulin secretion, and thus be beneficial to improve the weight gain rate of fish and the economic benefits of breeding.

[0105] Table 5 Effects of different feeds on growth performance and biochemical indicators of crucian carp

[0106]

[0107] Note: The same lowercase letters in the same row indicate no significant difference.

[0108] Application Example 6

[0109] Tilapia breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Animal Science Building of South China Agricultural University. Tilapia with an average weight of about 80g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating composition group. Each group had 4 circulating breeding tanks (specification: 500L), 30 fish in each tank, and the breeding cycle was 12 weeks. The feed formula was the same as shown in Table 1, but 1.2% of the regulating composition of Example 4 was used. After the breeding experiment, blood and liver samples were collected for index determination.

[0110] As shown in Table 6, after 12 weeks of breeding experiment, the weight gain rate of fish in the high sugar group was significantly lower than that in the control group (P<0.05), while the abdominal fat rate, liver fat content, and blood glucose level of fish in the high sugar group were significantly increased (P<0.05), indicating that fish fed with high sugar feed had liver fat accumulation, postprandial hyperglycemia symptoms, and the weight gain rate was significantly decreased. However, the liver fat content and blood glucose level of fish in the high sugar + regulation composition group were significantly lower than those in the high sugar group (P<0.05), but the weight gain rate and plasma insulin level were increased. This shows that the feed containing the regulation composition prepared in Example 4 can improve the liver fat accumulation caused by tilapia due to eating high sugar feed, enhance the blood glucose regulation ability of fish, and thus improve the weight gain rate of farmed tilapia.

[0111] Table 6 Effects of different feeds on growth performance and biochemical indicators of tilapia

[0112]

[0113] Note: The same lowercase letters in the same row indicate no significant difference.

[0114] Application Example 7

[0115] Largemouth bass breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Animal Science Building of South China Agricultural University. Largemouth bass with an average weight of about 5g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating combination group. Each group had 4 circulating breeding tanks (specification: 500L), 20 fish in each tank, and the breeding cycle was 12 weeks. The feed formula is shown in Table 7. After the breeding experiment, blood and liver samples were collected for index determination.

[0116] Figure 6The effects of different feeds on liver fat content of juvenile largemouth bass in Application Example 7 are shown in Table 8 and Table 9. Figure 6 As shown, the weight gain rate, blood sugar clearance ability and insulin sensitivity index of the high sugar group fish were significantly lower than those of the control group (P<0.05), while the abdominal fat rate, liver fat content, blood sugar level and blood sugar half-life of the high sugar group fish were significantly increased (P<0.05), indicating that the largemouth bass fed with high sugar feed had liver fat accumulation, reduced tissue insulin sensitivity and persistent high blood sugar, but the weight gain rate was significantly decreased. However, the liver fat content, blood sugar level and blood sugar half-life of the high sugar + regulation composition group fish were significantly lower than those of the high sugar group (P<0.05), but the weight gain rate and plasma insulin level were increased. This shows that the feed containing the regulation composition prepared in Example 5 can improve the liver fat accumulation caused by the largemouth bass due to the intake of high sugar feed, enhance the blood sugar clearance ability of the fish body and promote insulin secretion, thereby improving the weight gain rate of the fish body.

[0117] Table 7 Test feed formula

[0118]

[0119] Table 8 Effects of different feeds on growth performance and biochemical indices of largemouth bass

[0120]

[0121] Note: The same lowercase letters in the same row indicate no significant difference.

[0122] Application Example 8

[0123] Channel catfish breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Animal Science Building of South China Agricultural University. Channel catfish with an average weight of about 15g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating composition group. Each group had 4 circulating breeding tanks (specification: 500L), 25 fish in each tank, and the breeding cycle was 12 weeks. The feed formula is shown in Table 7, but 2.0% of the regulating composition of Example 6 was used. After the breeding experiment, blood and liver samples were collected for index determination.

[0124] As shown in Table 9, after 12 weeks of breeding experiment, the weight gain rate of fish in the high sugar group was significantly lower than that in the control group (P<0.05), while the abdominal fat rate, liver fat content, and blood sugar level of the high sugar group were significantly increased (P<0.05), indicating that the high sugar group fed with high sugar feed had liver fat accumulation, postprandial hyperglycemia symptoms, and the weight gain rate was significantly decreased. However, the liver fat content and blood sugar level of fish in the high sugar + regulation composition group were significantly lower than those in the high sugar group (P<0.05), but the weight gain rate and plasma insulin level were increased. This shows that the feed containing the regulation composition prepared in Example 6 can improve the liver fat accumulation caused by the intake of high sugar feed in the high sugar group, enhance the blood sugar regulation ability of the fish, and improve the weight gain rate of cultured tilapia.

[0125] Table 9 Effects of different feeds on growth performance and biochemical indicators of tilapia

[0126]

[0127] Note: The same lowercase letters in the same row indicate no significant difference.

[0128] Application Example 9

[0129] Black snakehead fish breeding experiment and result analysis: The breeding experiment was carried out in the indoor recirculating breeding system of the Animal Science Building of South China Agricultural University. Black snakehead fish with an average weight of about 35g were selected and randomly divided into three experimental groups, namely the control group, the high sugar group and the high sugar + regulating composition group. Each group had 4 circulating breeding tanks (specification: 500L), 30 fish in each tank, and the breeding cycle was 12 weeks. The feed formula is shown in Table 7, but 1.3% of the regulating composition of Example 8 was used. After the breeding experiment, blood and liver samples were collected for index determination.

[0130] The remaining fish were subjected to a glucose tolerance test, with the following specific steps: the fish were starved for 24 hours and then anesthetized; then, 1.67 g / kg body weight of glucose solution was injected intraperitoneally; after the injection, the fish were placed in 28 indoor circulating water tanks, i.e., 5 experimental groups, 7 tanks in each group (corresponding to 7 sampling points); then, blood samples were collected at 0, 1, 2, 4, 8 and 12 hours. One tank was sampled at each time point for each group to avoid the influence of density changes and reduce the stress caused by repeated sampling on the fish.

[0131] Figure 7 The following table shows the effects of different feeds on the blood sugar clearance ability of Channa argus in Application Example 9. The same lowercase letters in the figure indicate that the difference is not significant. Figure 7As shown, the weight gain rate, blood sugar clearance ability and insulin sensitivity index of the fish in the high sugar group feed were significantly lower than those in the control group (P<0.05), while the abdominal fat rate, liver fat content, blood sugar level and blood sugar half-life of the fish in the high sugar group were significantly increased (P<0.05), indicating that the black snakehead fed with high sugar feed had liver fat accumulation, reduced tissue insulin sensitivity and persistent high blood sugar, but the weight gain rate was significantly decreased. However, the liver fat content, blood sugar level and blood sugar half-life of the fish in the high sugar + regulation composition group were significantly lower than those in the high sugar group (P<0.05), but the weight gain rate and plasma insulin level were increased. This shows that the feed containing the regulation composition prepared in Example 8 can improve the liver fat accumulation caused by eating high sugar feed in black snakehead, enhance the blood sugar clearance ability of fish and promote insulin secretion, thereby improving the weight gain rate of fish.

[0132] Table 10 Effects of different feeds on growth performance and biochemical indices of Channa argus

[0133]

[0134] Note: The same lowercase letters in the same row indicate no significant difference.

[0135] Figure 8 This is the dynamic change curve of blood sugar of Channa argus in Application Example 9, which studies the effect of different feeds on glucose tolerance of Channa argus. Figure 8 It can be seen that after glucose loading, the maximum plasma glucose level of fish in each test group appeared at 2 hours, and showed a downward trend with the increase of sampling time. The blood glucose level of fish in the high sugar + regulation combination group was significantly lower than that of other test groups throughout the sampling time, and returned to the basic value at 12 hours. This shows that the high sugar + regulation combination group feed can significantly enhance the glucose tolerance of black snakehead.

[0136] In summary, the present invention, combined with aquaculture experiments, deeply explored the appropriate effects and dosages of the regulatory composition in feed, as well as its regulatory mechanism on the sugar metabolism function of herbivorous (grass carp and bighead bream), omnivorous (carp, crucian carp, tilapia and channel catfish) and carnivorous (largemouth bass and black snakehead) fish at different growth stages. Therefore, the regulation of sugar metabolism function of aquatic animals is highly targeted.

[0137] The present invention combines the growth and physicochemical index results of relevant breeding experiments, confirming that the regulatory composition can significantly improve the lipid accumulation and inflammatory response of fish metabolic tissues induced by high-sugar feed, and enhance their glucose and lipid metabolism function; significantly enhance the ability of fish to synthesize and secrete insulin, reduce the postprandial blood sugar level of fish, and relieve the metabolic stress of fish; significantly improve the sugar tolerance of fish, improve their utilization rate of sugars in feed, thereby reducing the feed conversion rate and increasing the economic benefits of breeding.

[0138] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A fishery control composition with lipid-lowering and blood sugar-controlling functions, characterized in that: The composition comprises the following components in parts by weight: 2-50 parts of berberine; 4-50 parts of allicin; 2-30 parts of benfotiamine; 2-50 parts of gamma-aminobutyric acid; 4-30 parts of sodium oxalate; 200-530 parts of gamma-glutamyl-phenylalanine; Among them, γ-glutamyl-phenylalanine is prepared by washing cotton stalks and corn stalks with distilled water, drying them, and then crushing them into a mixture of 80-120 mesh. The mixture is added with Mandel salt solution and then inoculated into a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens. The pH of the mixture is adjusted to 8-10, and the mixture is cultured at a constant temperature of 35-50°C for 2-4 days. The obtained enzyme fermentation liquid is centrifuged at 1000-1300r / min for 15-30min, and the supernatant is rotary evaporated at 105-150°C to obtain the product.

2. The fishery regulating composition with lipid-lowering and blood sugar-controlling functions according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 10-45 parts of berberine, 15-40 parts of allicin, 8-25 parts of benfotiamine, 10-43 parts of gamma-aminobutyric acid, 7-25 parts of sodium oxalate, and 260-500 parts of gamma-glutamyl-phenylalanine.

3. The fishery regulating composition with lipid-lowering and blood sugar-controlling functions according to claim 2, characterized in that: The composition comprises the following components in parts by weight: 20-40 parts of berberine, 17-35 parts of allicin, 10-20 parts of benfotiamine, 15-40 parts of gamma-aminobutyric acid, 10-20 parts of sodium oxalate, and 300-470 parts of gamma-glutamyl-phenylalanine.

4. The fishery regulating composition with lipid-lowering and blood sugar-controlling functions according to claim 3, characterized in that: The composition comprises the following components in parts by weight: 23-38 parts of berberine, 20-33 parts of allicin, 13-18 parts of benfotiamine, 30-39 parts of gamma-aminobutyric acid, 12-16 parts of sodium oxalate, and 350-450 parts of gamma-glutamyl-phenylalanine.

5. The method for preparing the fishery regulating composition with lipid-lowering and blood sugar-controlling functions according to any one of claims 1 to 4, characterized in that: The specific steps include: S1. Wash cotton stalks and corn stalks with distilled water, dry them in the sun, and then grind them into a mixture of 80-120 mesh. Add Mandel salt solution to the mixture and then inoculate it into a mixed bacterial solution of Aspergillus oryzae and Bacillus amyloliquefaciens. Adjust the pH of the mixture to 8-10, culture it at a constant temperature of 35-50°C for 2-4 days, centrifuge the obtained enzyme fermentation liquid at 1000-1300r / min for 15-30min, take the supernatant and rotary evaporate it at 105-150°C to obtain γ-glutamyl-phenylalanine; S2. After berberine, allicin, benfotiamine, γ-aminobutyric acid, sodium oxalate and γ-glutamyl-phenylalanine were crushed separately, passed through a 120-150 mesh sieve and then fully mixed to obtain a coarsely controlled composition; S3. Add β-cyclodextrin and dispersant to methanol aqueous solution and homogenize at a speed of 10000-12000 r / min for 10-15 min. Add methanol solution of the crude regulating composition with a mass concentration of 10-15 wt% under stirring at 1000-1300 r / min. Stir at 30-65°C and 1000-1300 r / min for 4-5 h. Cool naturally to room temperature and stand for 24-36 h. Filter and wash, cool and dry to obtain a fishery regulating composition with lipid-lowering and blood sugar-control properties.

6. The method for preparing the fishery control composition with lipid-lowering and blood sugar-controlling functions according to claim 5, characterized in that: The mass ratio of cotton stalks to corn stalks in step S1 is (1-2.5): (3-5.4), the mass ratio of Aspergillus oryzae to Bacillus amyloliquefaciens in the mixed bacterial solution is (2.5-3): (1-2.5); the mass ratio of the mixture to the volume ratio of Mandel salt solution is (1-2) g: (2-5) mL.

7. The method for preparing the fishery control composition with lipid-lowering and blood sugar-controlling functions according to claim 5, characterized in that: In step S2, the temperature of the berberine, allicin, benfotiamine, γ-aminobutyric acid, sodium oxalate and γ-glutamyl-phenylalanine during pulverization is all less than 40°C.

8. The method for preparing the fishery control composition with lipid-lowering and blood sugar-controlling functions according to claim 5, characterized in that: The dispersant in step S3 is a mixture of calcium lignin sulfonate and polyvinyl pyrrolidone K17 in a weight ratio of 2:1, the methanol aqueous solution is a mixed solution of methanol and distilled water in a volume ratio of 1:3; the mass ratio of the β-cyclodextrin and the dispersant is 3:(3.5-5); the volume ratio of the total mass of the β-cyclodextrin and the dispersant to the methanol aqueous solution is (400-500) g:(1-2.5) L; the volume ratio of the methanol aqueous solution to the methanol solution of the coarse control composition is 20:(1-1.5).

9. Use of the fishery regulating and controlling composition with lipid-lowering and blood sugar-controlling functions according to any one of claims 1 to 4 in the preparation of freshwater fish breeding feed.

10. The use of the fishery regulating composition with lipid-lowering and blood sugar-controlling functions according to claim 9 in preparing freshwater fish breeding feed, characterized in that: The addition amount of the regulating composition in the freshwater fish feed is 0.5-1.4 wt%; the freshwater fish is grass carp, bighead bream, carp, crucian carp, tilapia, channel catfish, largemouth bass or snakehead.

Images