An ECMO combined blood purification device for animals

Abstract

This invention discloses an ECMO combined with blood purification device for animals and its usage method, relating to the field of medical device technology. It includes: an extracorporeal blood circulation pipeline; a first driving metering element and an oxygenator, the first driving metering element and the oxygenator being connected in series on the extracorporeal blood circulation pipeline, and the first driving metering element and the oxygenator being sequentially distributed along the blood flow direction within the extracorporeal blood circulation pipeline; and a branch circulation pipe, the first end of which is connected to the input end of the oxygenator. This invention, by connecting the adsorption column and the oxygenator in parallel, eliminates the need for pipe joints at the input end of the first driving metering element, thereby reducing the risk of air entering the main circulation pipe or branch circulation pipe during experiments. Simultaneously, through the above parallel connection, in the event of oxygenator blockage, blood can flow back through the adsorption column, avoiding the problems of excessive blood loss and death in small animals or excessive blood dilution.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to an ECMO combined blood purification device for animals and a method of using the same. Background Art

[0002] Extracorporeal membrane oxygenation (ECMO) is an extension and expansion of extracorporeal circulation technology in clinical use. Its essence is an artificial heart-lung machine, which achieves the treatment purpose by fully or partially replacing the functions of the heart and lungs to reduce the pressure on the patient's own heart and lungs, thereby gaining recovery opportunities.

[0003] in, Figure 1 ECMO combined with blood purification device for human use. Figure 1 As shown, the adsorption column is connected in parallel with the power pump. When the experiment is carried out (due to the large negative pressure at the input end of the power pump), air easily enters the pipeline from the connection gap between the sub-circulation pipe and the main circulation pipe. When excessive bubbles are generated in the pipeline and enter the animal's body circulation, air embolism will be formed, resulting in the death of the animal. At the same time, the oxygenator is connected in series to the main circulation pipeline. When the oxygenator is blocked or the blood flow resistance is large, the animal's blood cannot be transported back to the animal's body in time. In order to prevent the presence of gas in the ECMO extracorporeal pipeline, the pipeline will be pre-filled before the flow is switched, and the pre-filled liquid in the liquid storage part will be added to the main circulation pipeline. When the experimental animal is small (for example: rats), its whole blood volume is also small. The pre-flushing liquid in the pipeline can easily cause excessive dilution of the rat's blood, affecting the metabolism of tissues and organs, and in severe cases, it can cause the death of the animal. The above phenomenon will cause large errors in the experimental data at the least, and cause the death of the animal and lead to the failure of the experiment at the worst. Summary of the invention

[0004] The object of the present invention is to provide an ECMO combined blood purification device for animals and a method of using the same, so as to prevent technical problems such as excessive dilution of animal blood and generation of bubbles in the pipeline during animal experiments.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an ECMO combined blood purification device for animals, comprising: an extracorporeal blood circulation pipeline; a first drive metering component and an oxygenator, wherein the first drive metering component and the oxygenator are installed in series on the extracorporeal blood circulation pipeline, and the first drive metering component and the oxygenator are distributed sequentially along the blood flow direction in the extracorporeal blood circulation pipeline; a branch circulation pipe, wherein the first end of the branch circulation pipe is connected to the input end of the oxygenator, and the second end of the branch circulation pipe is connected to the output end of the oxygenator; and an adsorption column, wherein the adsorption column is installed on the branch circulation pipe.

[0006] Preferably in the present technical solution, the extracorporeal blood circulation circuit includes: a main circulation tube; a blood collection piece, which is arranged at the first end of the main circulation tube and is used to connect the animal's blood drawing blood vessel with the main circulation tube; and a blood return piece, which is arranged at the second end of the main circulation tube and is used to connect the animal's blood return blood vessel with the main circulation tube.

[0007] Preferably in the present technical solution, the inner diameter of the main circulation pipe is greater than or equal to 1.6 mm and less than or equal to 3.2 mm; the inner diameter of the branch circulation pipe is greater than or equal to 0.5 mm and less than or equal to 1.5 mm.

[0008] Preferably, the technical solution further comprises a liquid storage element, wherein the liquid storage element is used to store a blood diluent, and the liquid storage element is connected to an input end of the first driving metering element.

[0009] Preferably, the technical solution further comprises a second driving metering member, wherein the second driving metering member is installed on the sub-circulation pipe and is connected in series with the adsorption column.

[0010] Preferably in the present technical solution, the first end of the branch circulation pipe is connected to the input end of the oxygenator through a three-way pipe, the second end of the branch circulation pipe is connected to the output end of the oxygenator through another three-way pipe, and a control valve is provided on each pipeline of the two three-way pipes.

[0011] Based on the above-mentioned ECMO combined blood purification device for animals, the present invention also proposes an experimental method for ECMO combined blood purification of animals, the method comprising: using any one of the above-mentioned ECMO combined blood purification devices to conduct an ECMO combined blood purification experiment on animals.

[0012] Preferably in this technical solution, the method comprises:

[0013] S100: connecting the two ends of the extracorporeal blood circulation tube to the blood drawing blood vessel and the blood returning blood vessel of the animal respectively to form a blood circulation loop;

[0014] S200: Turn on the first driving metering component, the oxygenator and the second driving metering component respectively, wherein the first driving metering component pumps the blood of the animal from the blood drawing vessel to the blood returning vessel, and the second driving metering component pumps the blood of the animal from the blood returning vessel to the blood drawing vessel.

[0015] Preferably in the technical solution, after the step S200, the method further includes:

[0016] S300: monitoring the real-time flow of the first driving metering element and the second driving metering element, and calculating the actual oxygenated blood volume;

[0017] S400: If the actual oxygenated blood volume is greater than the oxygenated blood volume threshold range, the power of the second driving metering component is reduced; if the actual oxygenated blood volume is less than the oxygenated blood volume threshold range, the power of the second driving metering component is increased.

[0018] Preferably in the technical solution, after the step S200, the method further includes:

[0019] S500: If the oxygenator is blocked or damaged, replace the oxygenator;

[0020] S600: If the adsorption column is over-adsorbed or damaged, replace the adsorption column;

[0021] in:

[0022] The step S500 includes:

[0023] S510: adjusting the second driving metering element so that the blood in the sub-circulation pipe can flow from the first end to the second end of the sub-circulation pipe, and then closing the control valves on the pipes at both ends of the oxygenator;

[0024] S520: Remove the old oxygenator and install the new oxygenator;

[0025] S530: Open the control valves on the pipelines at both ends of the oxygenator, and then adjust the second driving metering element so that the blood in the sub-circulation pipe can flow from the second end to the first end of the sub-circulation pipe;

[0026] The step S600 includes:

[0027] S610: closing the second driving metering element, and then closing the control valves on both ends of the branch circulation pipe;

[0028] S620: dismantling the old adsorption column and installing a new adsorption column;

[0029] S630: Open the control valves on both ends of the branch circulation pipe, and open the second driving metering component.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The ECMO combined blood purification device for animals, by connecting the adsorption column and the oxygenator in parallel, makes the input end of the first driving metering member free of pipe joints, thereby reducing the risk of air inflow into the main circulation pipe or the branch circulation pipe during the experiment. At the same time, through the above-mentioned parallel connection, when the oxygenator is blocked, the blood can flow back through the adsorption column, avoiding the problem of excessive blood loss and death or excessive blood dilution of small animals. Furthermore, it is conducive to ensuring the stability of animal experiments and improving the success rate of experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a structural schematic diagram of the prior art solution;

[0033] Figure 2 for Figure 1 Schematic diagram of blood flow in the main circulation tube and the branch circulation tube;

[0034] Figure 3 A schematic diagram of the structure of an embodiment of the present invention;

[0035] Figure 4 for Figure 2 Schematic diagram of blood flow in the main circulation tube and the branch circulation tube;

[0036] Figure 5 A schematic diagram of the structure of another embodiment of the present invention;

[0037] Figure 6 for Figure 4 Schematic diagram of blood flow in the main circulation tube and the branch circulation tube;

[0038] Figure 7 is a first flow chart of the method of the present invention;

[0039] Figure 8 This is a second flow chart of the method of the present invention.

[0040] In the figure: 1. blood collection component; 2. main circulation tube; 3. first drive metering component; 4. oxygenator; 6. liquid storage component; 7. branch circulation tube; 8. adsorption column; 9. second drive metering component; 10. blood return component; 11. three-way pipe; 12. power pump. DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] It should be noted that, in the description of the present invention, the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0043] It should be noted that like reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined or described in one drawing, it will not require further detailed discussion and description in the description of the subsequent drawings.

[0044] Before understanding the present invention, it should be clear that extracorporeal membrane oxygenation mainly includes: intravascular cannula, connecting tube, power pump, oxygenator and other parts. Among them, the power pump is used to replace the artificial heart, and the oxygenator is used to replace the artificial lung. The principle is: first, the human blood is transported to the oxygenator through the power pump; then, the blood undergoes gas exchange in the oxygenator; finally, the blood carrying oxygen is transported back to the human body, thereby realizing the patient's extracorporeal breathing and circulation to maintain the patient's life.

[0045] Therefore, from the above, it can be seen that extracorporeal membrane oxygenation is mainly suitable for the following aspects: patients with cardiac arrest, patients with acute severe heart failure, patients with acute severe respiratory failure, other diseases that seriously threaten respiratory and circulatory functions, as well as organ transplant support, patients waiting for donors, etc.

[0046] Sepsis generally causes acute severe respiratory failure in patients. At the same time, septic patients will have cytokine storms, which will lead to a variety of complications in the later stage, mainly including acute respiratory distress syndrome (ARDS) with a high mortality rate. As a common clinical critical illness, it is the main cause of death in ICU patients worldwide, and can cause 10 million deaths worldwide each year. Extracorporeal membrane oxygenation support brings a turning point for the treatment of severe ARDS patients. However, extracorporeal membrane oxygenation only has a supporting function, which can buy more time for the treatment of ARDS patients, and cannot treat ARDS, and complications such as inflammatory reactions will be produced due to in vitro intervention during the support process. Therefore, there is currently an adsorption column added to the extracorporeal membrane oxygenation device on the market to try to remove new devices for inflammatory factors, that is, ECMO combined blood purification devices. However, in order to study the effect of ECMO combined blood purification in the treatment of ARDS and explore the optimal intervention time and adsorption duration of inflammatory factor adsorption, animal experiments need to be carried out for further research. At present, there are few related animal studies. For this reason, the present invention provides a stable and reliable ECMO combined blood purification device for animals, which is used to ensure the stability of animal experiments and improve the success rate of experiments.

[0047] Before understanding the present invention, it is also necessary to understand that Figure 1As shown, it is an ECMO combined blood purification device applied to human body in the prior art. It includes a main circulation tube 2, one end of which is connected to a blood collection part 1, and the other end is connected to a blood return part 10. And a power pump 12 and an oxygenator 4 are installed on the main circulation tube 2 in sequence according to the flow direction of blood. The adsorption column 8 is connected in parallel with the power pump 12 on the main circulation tube 2 through the branch circulation tube 7. And the input end of the power pump 12 is also connected to a liquid storage part 6, which mainly stores blood, physiological saline or medicine for treating patients.

[0048] And as Figure 1 The ECMO combined blood purification device for human body shown is not suitable for smaller animals. First, small animals (such as mice) are small in size, and after the power pump 12 is turned on, the input end of the power pump 12 is in a negative pressure state, which can easily lead to air intake at the connection interface (i.e., the three-way pipe 11) between the branch circulation tube 7 and the input end of the power pump 12. For small animals, a small amount of gas is also fatal, resulting in distortion of experimental data or failure of the experiment. Secondly, the total amount of blood in small animals is limited. When conducting experiments on small animals, in order to prevent the small animals from dying due to blood being drained, it is necessary to add blood diluent to the liquid storage part 6. If the negative pressure at the input end of the power pump 12 is too large, the blood diluent in the liquid storage part 6 can be automatically added to the main circulation tube 2. Figure 2 As shown, part of the blood passing through the power pump 12 returns to the small animal through the oxygenator 4 (i.e. Figure 2 In the direction E), since the output end of the power pump 12 is in a positive pressure state, another part of the blood passes through the adsorption column 8 and returns from the output end of the power pump 12 to the input end of the power pump 12 (that is, Figure 2 In the direction F). If the resistance of the oxygenator 4 used in the experiment is too large, or the oxygenator 4 is blocked by a thrombus and causes too large resistance, the blood forms an external circulation between the power pump 12 and the adsorption column 8, which may easily cause the small animal to die of hypoxia. In addition, if the amount of blood returning to the small animal through the oxygenator 4 is small, the main circulation tube 2 before the input end of the power pump 12 is in a negative pressure state for a long time, which may easily cause excessive dilution of the blood.

[0049] In summary, in order to solve the technical problems existing in the above-mentioned prior art, the present invention provides a technical solution: an ECMO combined blood purification device for animals, such as Figure 3As shown, it includes: an extracorporeal blood circulation pipeline, a first driving metering component 3, an oxygenator 4, a branch circulation pipe 7 and an adsorption column 8. The first driving metering component 3 and the oxygenator 4 are installed in series on the extracorporeal blood circulation pipeline, and the first driving metering component 3 and the oxygenator 4 are sequentially distributed along the blood flow direction in the extracorporeal blood circulation pipeline. The branch circulation pipe 7 is connected in parallel with the extracorporeal blood circulation pipeline, the first end of the branch circulation pipe 7 is connected to the input end of the oxygenator 4, the second end of the branch circulation pipe 7 is connected to the output end of the oxygenator 4, and the adsorption column 8 is installed on the branch circulation pipe 7.

[0050] It should be clear that in the above embodiments, Figure 3 As shown, first, the connection interface between the sub-circulation pipe 7 and the main circulation pipe 2 is located after the output end of the first driving metering member 3. In other words, the connection interface between the sub-circulation pipe 7 and the main circulation pipe 2 is always in a positive pressure state, so the outside gas cannot enter the main circulation pipe 2 from this interface. Figure 4 As shown, Figure 4 FIG. 2 is a schematic diagram of the blood flow in the main circulation tube 2 and the branch circulation tube 7 in the above embodiment. Figure 4 It can be seen that the blood in the main circulation tube 2 flows from the blood collection unit 1 to the blood return unit 10 (i.e. Figure 4 The blood in the circulation tube 7 flows from the blood collection unit 1 to the blood return unit 10 (i.e. Figure 4 Therefore, the blood in the main circulation tube 2 and the branch circulation tube 7 flows in the same direction, that is, from the blood collection part 1 to the blood return part 10. Therefore, the blood can enter the body of the small animal through the adsorption column 8 or the oxygenator 4, thereby effectively avoiding the technical problem of excessive dilution of the blood caused by less blood return of the small animal.

[0051] At the same time, it should be clear that in this embodiment, the extracorporeal blood circulation pipeline can be any common pipeline that can be used for extracorporeal blood circulation. Figure 3 As shown, in one embodiment of the present invention, the extracorporeal blood circulation circuit includes: a main circulation tube 2, a blood sampling component 1 and a blood return component 10. The blood sampling component 1 is arranged at a first end of the main circulation tube 2, and is used to connect the blood drawing blood vessel of the animal with the main circulation tube 2. The blood return component 10 is arranged at a second end of the main circulation tube 2, and is used to connect the blood return blood vessel of the animal with the main circulation tube 2.

[0052] In the present invention, the main circulation tube 2 can be composed of one section of pipeline or multiple sections of pipeline. If the main circulation tube 2 is composed of one section of pipeline, each component (for example: blood sampling component 1, first driving metering component 3, oxygenator 4, adsorption column 8 and blood return component 10, etc.) can be designed as an integrated structure with the main circulation tube 2. However, in order to facilitate the use of daily experiments, such as Figure 3As shown, in a specific embodiment of the present invention, the main circulation pipe 2 is mainly composed of multiple sections of pipelines, which are connected to the blood sampling component 1, the first driving metering component 3, the oxygenator 4 and the blood return component 10 in sequence according to the flow direction of the blood, and are connected in parallel using a three-way pipe 11. Of course, in other embodiments of the present invention, other connection structures can also be used instead of the three-way pipe 11.

[0053] As can be seen from the above, in the present invention, the blood collection piece 1 is used to connect the blood drawing blood vessel of the animal with the main circulation tube 2. And the blood return piece 10 is used to connect the blood return blood vessel of the animal with the main circulation tube 2. That is, both the blood collection piece 1 and the blood return piece 10 need to be inserted into the body of the animal. Therefore, it is easy to associate that in the embodiment of the present invention, the blood collection piece 1 and the blood return piece 10 can be any medical device used to be inserted into the human body for blood collection or blood transfusion. For example: a blood collection needle, an intravascular cannula, a butterfly wing needle, a scalp needle, a double-wing needle or a straight needle head, etc., which are not described one by one here. At the same time, in other embodiments of the present invention, the blood collection piece 1 and the blood return piece 10 can also be designed as an integrated device. For example: a double-cavity venous cannula, etc.

[0054] It should also be clear that in the present invention, the first drive metering member 3 refers to a device with a drive function and a metering function (the second drive metering member 9 hereinafter is also the same). The drive function is to drive the blood as described above, so that the blood can form an extracorporeal circulation in the main circulation tube 2. And the metering function refers to the ability to measure the blood flow through the first drive metering member 3. Therefore, it is easy to associate that the first drive metering member 3 can be any device with metering and conveying functions, such as a peristaltic pump and a metering pump. Of course, in other embodiments of the present invention, the first drive metering member 3 can also be formed by combining a device with a conveying function and a device with a metering function. Among them, the device with a conveying function includes but is not limited to a roller pump, a centrifugal pump, a plunger pump, a peristaltic pump and a metering pump, and the device with a metering function includes but is not limited to a flow meter, a flow meter and a flow meter. Of course, it is easy to understand that the device with a conveying function in the present invention is not limited to a pump, but can be any other device capable of liquid conveying.

[0055] As can be seen from the above, the main function of the oxygenator 4 in the present invention is to oxygenate the input blood and output the oxygenated blood. The types of oxygenators 4 are also various, including membrane oxygenators and bubbling oxygenators. Among them, the membrane oxygenator is a tube where blood flows outside and air flows inside, which can reduce the area of ​​contact between blood and foreign matter and reduce the pre-charge amount. Bubbling oxygenation is to directly blow oxygen into the blood to form countless small bubbles, and the blood is in direct contact with oxygen, so it has a good oxygenation effect. Among the specific embodiments of the present invention, the appropriate type of oxygenator 4 can be selected according to the implementation requirements without any restriction, and the implementation requirements include but are not limited to blood flow resistance and oxygenation efficiency.

[0056] It should be clear that in different blood purifications, the types of adsorption columns 8 used are also different. For example, commonly used blood purification adsorption columns on the market include hemodialysis filters, plasma exchange filters, DNA immunoadsorption columns, and hemoperfusion adsorption columns. In the present invention, there is no restriction on the type of adsorption column 8. Any type of adsorption column can be used to obtain the ECMO combined blood purification device for animals proposed in the embodiment of the present invention.

[0057] In another embodiment of the present invention, Figure 3 As shown, it also includes a liquid storage part 6, which is used to store blood diluent, and the liquid storage part 6 is connected to the input end of the first driving metering part 3. It should be clear that the blood diluent component in the present invention can be any one component or a combination of multiple components, and there is no restriction on the components of the blood diluent. Specifically, the components of the blood diluent in the present invention include but are not limited to physiological saline, animal blood, drugs for treating diseases and anticoagulants. At the same time, in the present invention, the liquid storage part 6 can be any container that can store liquid, such as a syringe and a plastic bag.

[0058] It should be clear that, in most cases, the resistance of the adsorption column 8 to blood flow is much smaller than the resistance of the oxygenator 4 to blood flow. Therefore, during the long-term use of the oxygenator 4, if a thrombus is formed inside it, the resistance to blood flow will further increase, which will cause most of the blood to flow back into the small animal from the branch circulation tube 7 and through the adsorption column 8, so that the blood flowing back into the small animal is not fully oxygenated, which may easily lead to the death of the small animal due to lack of oxygen.

[0059] Based on the above reasons, in another embodiment of the present invention, Figure 5 As shown, it also includes a second driving metering member 9, which is installed on the sub-circulation pipe 7 and connected in series with the adsorption column 8. The second driving metering member 9 is similar to the first driving metering member 3 described above, and will not be described in detail here.

[0060] The purpose of designing the second driving metering element 9 is to pump the blood at the output end of the oxygenator 4 to the input end of the oxygenator 4. Figure 6 As shown, when the above embodiment is used for the experiment, the blood in the main circulation tube 2 flows from the blood collection part 1 to the blood return part 10 (that is, Figure 6 The blood in the circulation tube 7 flows from the blood return part 10 to the blood collection part 1 (i.e. Figure 6 Of course, it is easy to understand that in other embodiments of the present invention, if the pumping pressure of the second driving metering member 9 is less than the blood pressure at the input end of the oxygenator 4, the blood flow direction in the subcirculation pipe 7 will also be different from Figure 6 The direction D in the oxygenator 4 is opposite to that in the oxygenator 4, or a second driving metering member 9 (e.g., a peristaltic pump) capable of bidirectional delivery is used, then the blood flow direction in the sub-circulation tube 7 can be controlled as required, which will not be described in detail here. It is easy to understand that through the above design, it is possible to effectively prevent the phenomenon of insufficient blood flowing into the oxygenator 4, insufficient blood oxygenation, and lack of oxygen and death of small animals. At the same time, it is easy to understand that the flow rate of blood flowing through the oxygenator 4 can be adjusted by the second driving metering member 9, thereby avoiding the problem of excessive blood oxygenation.

[0061] As can be seen from the above, the main function of the second driving metering element 9 is to increase (or adjust) the resistance of blood flow in the branch circulation tube 7. Therefore, there is no restriction on the installation position of the second driving metering element 9 and the adsorption column 8 in the branch circulation tube 7. Figure 6 In the embodiment, the second driving metering member 9 and the adsorption column 8 are arranged in sequence along the direction C. Of course, during the installation process, the second driving metering member 9 and the adsorption column 8 can also be arranged in sequence along the direction D.

[0062] Further, in another embodiment of the present invention, Figure 3 and Figure 5 As shown, the first end of the branch circulation pipe 7 is connected to the input end of the oxygenator 4 through a three-way pipe 11, and the second end of the branch circulation pipe 7 is connected to the output end of the oxygenator 4 through another three-way pipe 11, and each pipeline of the two three-way pipes 11 is provided with a control valve. During the experiment, if the oxygenator 4 is too blocked and cannot be used, the pipeline in the three-way pipe 11 connected to the oxygenator 4 is closed, and the blood in the main circulation pipe 2 can flow back to the body of the small animal through the branch circulation pipe 7. After the oxygenator 4 is replaced, the corresponding pipeline is opened again so that the experiment can continue.

[0063] It is easy to understand that when the adsorption column 8 needs to be replaced, the pipelines on the two three-way pipes 11 connected to the sub-circulation pipe 7 can be closed according to the same principle as above, so as to replace the adsorption column 8.

[0064] In the present invention, the small animals used for the experiment can be various, without any restriction on their types, such as mice and rabbits. Although the ECMO combined blood purification device for animals of the present invention is mainly designed for small animals, it does not mean that the device of the present invention can only be applied to small animals. In other embodiments of the present invention, it can also be applied to large animals, such as monkeys and pigs.

[0065] It should be clear that small animals are commonly used experimental subjects when conducting various medical experiments. In order to prevent the blood pre-filling volume required in the extracorporeal blood circulation circuit from being too large and excessive blood extraction from causing ischemia and death of small animals, the present invention also provides a specific embodiment, which is mainly aimed at mice or other animals with body shapes similar to mice. In the present invention, the inner diameter of the main circulation tube 2 is greater than or equal to 1.6mm and less than or equal to 3.2mm. Specifically, it can be any inner diameter of 1.6mm, 1.7mm, 1.8mm, 1.9mm, 2.0mm, 2.1mm, 2.2mm, 2.3mm, 2.4mm, 2.5mm, 2.6mm, 2.7mm, 2.8mm, 2.9mm, 3.0mm, 3.1mm and 3.2mm, or any inner diameter between the above adjacent inner diameters. In the present invention, the inner diameter of the sub-circulation pipe 7 is greater than or equal to 0.5 mm and less than or equal to 1.5 mm. Specifically, it can be any inner diameter of 0.6 mm, 0.7 mm, 0.8 mm, 0.9 mm, 1.0 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm and 1.5 mm, or any inner diameter between the above adjacent inner diameters.

[0066] Based on the above-mentioned ECMO combined blood purification device for animals, the present invention also proposes an experimental method for ECMO combined blood purification of animals, the method comprising: using the ECMO combined blood purification device in any of the above-mentioned embodiments to conduct an ECMO combined blood purification experiment on animals.

[0067] In a specific embodiment of the method of the present invention, Figure 7 As shown, the method includes:

[0068] S100: Connecting the two ends of the extracorporeal blood circulation pipeline to the blood drawing blood vessel and the blood returning blood vessel of the animal respectively to form a blood circulation loop.

[0069] Specifically, in the method of the present invention, the blood-drawing blood vessel refers to the blood vessel used to draw blood from an animal during an animal experiment, and the blood-returning blood vessel refers to the blood vessel used to transfuse blood into an animal during an animal experiment.

[0070] It is important to know that when ECMO is in operation, blood is generally drawn from the veins, oxygenated through the membrane lung, and carbon dioxide is discharged. The oxygenated blood can be returned to the veins (also known as VV bypass) or to the arteries (also known as VA bypass). VV bypass is mainly used for extracorporeal respiratory support. The venous blood has been partially exchanged with gases before flowing through the lungs to make up for the lack of lung function. VV bypass draws venous blood through the veins, oxygenates it through the oxygenator, and removes carbon dioxide before pumping it into another vein. Usually, the femoral vein is selected for extraction and the internal jugular vein is pumped in. The bilateral femoral veins can also be selected according to other circumstances. VA bypass can be used for both extracorporeal respiratory support and cardiac support. The power pump can replace the heart's pumping function to maintain blood circulation. VA bypass draws venous blood from the veins, oxygenates it through the oxygenator, and removes carbon dioxide before pumping it into the arteries. VA bypass is a connection method that supports both cardiopulmonary function and is suitable for studying conditions such as heart failure, severe lung failure, and possible cardiac arrest.

[0071] Therefore, it is clear that in the method of the present invention, the blood drawing vessel can be a vein, and the blood returning vessel can be an artery, or a vein different from the blood drawing vessel. Of course, in other embodiments of the present invention, the blood drawing vessel and the blood returning vessel can also be the same vein, for example, when a double-lumen venous cannula is used as the blood drawing unit 1 and the blood returning unit 10, the blood drawing vessel and the blood returning vessel are the same vein.

[0072] Before the experiment, the ECMO combined blood purification device can be installed in any of the above-mentioned ways. Specifically, in the method of the present invention, the ECMO combined blood purification device is installed as follows: Figure 5 shown.

[0073] S200: Turn on the first driving metering element 3, the oxygenator 4 and the second driving metering element 9 respectively, wherein the first driving metering element 3 pumps the blood of the animal from the blood drawing vessel to the blood returning vessel, and the second driving metering element 9 pumps the blood of the animal from the blood returning vessel to the blood drawing vessel.

[0074] Further, such as Figure 8 As shown, in one embodiment of the method of the present invention, after step S200, the method further includes:

[0075] S300: Monitor the real-time flow of the first driving metering element 3 and the second driving metering element 9 and calculate the actual oxygenated blood volume.

[0076] Specifically, Figure 5As shown in the figure, if the first driving metering member 3 and the second driving metering member 9 pump blood in the same direction, the amount of blood flowing through the oxygenator 4 (that is, the actual oxygenated blood volume) is equal to the flow rate of the first driving metering member 3 minus the flow rate of the second driving metering member 9. If the first driving metering member 3 and the second driving metering member 9 pump blood in opposite directions, the amount of blood flowing through the oxygenator 4 (that is, the actual oxygenated blood volume) is equal to the flow rate of the first driving metering member 3 plus the flow rate of the second driving metering member 9.

[0077] As can be seen from the above, the actual oxygenated blood volume can be controlled by increasing or decreasing the power of the second driving metering element 9. In other embodiments, further adjustment can be performed by even reversing the second driving metering element 9.

[0078] S400: If the actual oxygenated blood volume is greater than the oxygenated blood volume threshold range, the power of the second driving metering component is reduced; if the actual oxygenated blood volume is less than the oxygenated blood volume threshold range, the power of the second driving metering component is increased or the oxygenator is replaced.

[0079] It should be understood that the oxygenated blood volume threshold range refers to the range of blood volume flowing through the oxygenator 4 during the experiment.

[0080] Further, with Figure 1 Compared with the prior art, in the actual use process of the device of the present invention, the oxygenator 4 or the adsorption column 8 can be replaced. Therefore, in other embodiments of the method of the present invention, after step S200, the following steps are further included: S500: if the oxygenator 4 is blocked or damaged, the oxygenator 4 is replaced; S600: if the adsorption column 8 is over-adsorbed or damaged, the adsorption column 8 is replaced.

[0081] Specifically, step S500 includes:

[0082] S510: Adjust the second driving metering element 9 so that the blood in the branch circulation tube 7 can flow from the first end to the second end of the branch circulation tube 7, and then close the control valves on the pipelines at both ends of the oxygenator 4.

[0083] Among them, in the method of the present invention, adjusting the second driving metering member 9 means turning off the second driving metering member 9 or making the second driving metering member 9 rotate in the opposite direction. For example, if some pumps that can rotate in the opposite direction under the action of liquid pressure are used as the second driving metering member 9 (for example, a fan pump), the second driving metering member 9 is turned off, and the blood in the branch circulation tube 7 can flow from the first end to the second end of the branch circulation tube 7 under the action of the blood pressure itself. If some pumps that cannot rotate in the opposite direction under the action of liquid pressure are used as the second driving metering member 9 (for example, a peristaltic pump), the second driving metering member 9 is turned off, and the blood in the branch circulation tube 7 cannot flow from the first end to the second end of the branch circulation tube 7 under the action of the blood pressure itself, then it is necessary to control the reverse drive of the second driving metering member 9 so that the blood in the branch circulation tube 7 can flow from the first end to the second end of the branch circulation tube 7.

[0084] S520: Remove the old oxygenator 4 and install a new oxygenator 4.

[0085] S530: Open the control valves on the pipelines at both ends of the oxygenator 4, and then adjust the second driving metering member 9 so that the blood in the branch circulation pipe 7 can flow from the second end to the first end of the branch circulation pipe 7.

[0086] Specifically, step S600 includes:

[0087] S610: close the second driving metering member 9, and then close the control valves on both ends of the branch circulation pipe 7;

[0088] S620: dismantling the old adsorption column 8 and installing a new adsorption column 8;

[0089] S630: Open the control valves on both ends of the sub-circulation pipe 7, and open the second driving metering member 9.

[0090] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An ECMO combined blood purification device for animals, characterized in that: include: Extracorporeal blood circulation circuit; A first driving metering element (3) and an oxygenator (4), wherein the first driving metering element (3) and the oxygenator (4) are installed in series on the extracorporeal blood circulation pipeline, and the first driving metering element (3) and the oxygenator (4) are sequentially distributed along the blood flow direction in the extracorporeal blood circulation pipeline; A branch circulation pipe (7), wherein a first end of the branch circulation pipe (7) is connected to an input end of the oxygenator (4), and a second end of the branch circulation pipe (7) is connected to an output end of the oxygenator (4); An adsorption column (8), wherein the adsorption column (8) is installed on the sub-circulation pipe (7); A second driving metering member (9), the second driving metering member (9) is installed on the sub-circulation pipe (7) and is connected in series with the adsorption column (8); the second driving metering member (9) is capable of bidirectional transportation; The second driving metering element (9) is used to control the actual oxygen content by increasing or decreasing its own power, or by reversing the power.

2. The ECMO combined blood purification device for animals according to claim 1, characterized in that: The extracorporeal blood circulation circuit comprises: Main circulation pipe (2); A blood sampling piece (1), the blood sampling piece (1) being arranged at a first end of the main circulation tube (2) and used for connecting a blood sampling blood vessel of an animal with the main circulation tube (2); A blood return piece (10), the blood return piece (10) is arranged at the second end of the main circulation tube (2) and is used to connect the animal's blood return blood vessel with the main circulation tube (2).

3. The ECMO combined blood purification device for animals according to claim 2, characterized in that: The inner diameter of the main circulation pipe (2) is greater than or equal to 1.6 mm and less than or equal to 3.2 mm; the inner diameter of the branch circulation pipe (7) is greater than or equal to 0.5 mm and less than or equal to 1.5 mm.

4. The ECMO combined blood purification device for animals according to claim 1, characterized in that: It also comprises a liquid storage element (6), wherein the liquid storage element (6) is used to store a blood diluent, and the liquid storage element (6) is connected to the input end of the first driving metering element (3).

5. The ECMO combined blood purification device for animals according to claim 1, characterized in that: The first end of the sub-circulation pipe (7) is connected to the input end of the oxygenator (4) through a three-way pipe (11), and the second end of the sub-circulation pipe (7) is connected to the output end of the oxygenator (4) through another three-way pipe (11), and each pipe of the two three-way pipes (11) is provided with a control valve.

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