An exosome composition and its application in preparing a product with anti-allergic effect

Abstract

The present invention relates to the technical field of skin care products, and specifically provides a composition containing exosomes. The composition comprises the following components in parts by weight: 4-6 parts of umbilical cord mesenchymal stem cell exosomes, 1-3 parts of onion ginger mixed extract, 1-3 parts of anti-inflammatory liposomes, 0.5-1.5 parts of hyaluronic acid, and 3-6 parts of oat extract. The umbilical cord mesenchymal stem cell exosomes play a major anti-allergic role in the composition of the present invention. The exosomes are encapsulated by anti-inflammatory liposomes and supplemented with anti-inflammatory substances such as onion ginger mixed extract and oat extract. The anti-allergic effect is significantly enhanced, the skin barrier can be repaired, inflammation can be reduced, and the skin can be restored to a stable state.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an exosome composition and an application thereof in preparing a product with anti-allergic effect. Background Art

[0002] Many surveys show that most people have experienced skin allergies. Allergies are a common skin problem and many reasons may lead to skin allergies, such as allergies caused by allergens such as pollen and food, allergies caused by fragile and dry skin due to seasonal changes, allergies caused by contact with items containing too many bacteria, etc. Skin symptoms such as itching, redness, swelling, peeling, and bulging are ultimately caused by inflammation of the epidermis and superficial dermis, causing physical and mental distress. At this time, many skin care products are no longer suitable for allergic skin. Abuse of skin care products may aggravate allergies and create a heavier burden on the skin. Many anti-allergic products on the market have a counterproductive effect. Skin care products do not have a real anti-allergic effect. Instead, they irritate the skin due to their own irritation.

[0003] To reduce and eliminate allergies, it is necessary to inhibit the skin's immune response, such as the rupture of large cells to release active substances such as histamine that cause allergies; at the same time, it is necessary to strengthen the skin barrier and repair the sebum membrane so that it can normally play its role in locking in moisture and protecting it. Summary of the invention

[0004] Purpose of the invention: To solve the above-mentioned problem, the present invention adds a variety of effective anti-allergic substances, and finds that they play a coordinated role. Under the action of multiple active anti-allergic substances, the anti-allergic and anti-inflammatory effects of the composition are significantly improved;

[0005] The core of the invention is that the invention adds umbilical cord mesenchymal stem cell exosomes as the main anti-allergic component, and adds anti-inflammatory liposomes to encapsulate it, thereby maintaining its biological activity and enhancing the anti-allergic and anti-inflammatory effects;

[0006] The onion-ginger mixed extract prepared by the present invention adopts mixed extraction technology to effectively retain the anti-allergic active ingredients in onion and ginger. Experiments show that it has stronger anti-allergic and anti-inflammatory effects than single extraction.

[0007] The technical solution of the present invention is as follows:

[0008] The present invention provides an exosome-containing composition, comprising the following components in parts by weight: 4-6 parts of umbilical cord mesenchymal stem cell exosomes, 1-3 parts of onion and ginger mixed extract, 1-3 parts of anti-inflammatory liposomes, 0.5-1.5 parts of hyaluronic acid, and 3-6 parts of oat extract.

[0009] According to further research, the exosome-containing composition comprises the following components in parts by weight: 4-5 parts of umbilical cord mesenchymal stem cell exosomes, 2-3 parts of onion and ginger mixed extract, 2-3 parts of anti-inflammatory liposomes, 1-1.5 parts of hyaluronic acid, and 4-6 parts of oat extract.

[0010] According to further research, the exosome-containing composition is characterized in that it includes the following components in parts by weight: 5 parts of umbilical cord mesenchymal stem cell exosomes, 2 parts of onion and ginger mixed extract, 2 parts of anti-inflammatory liposomes, 1 part of hyaluronic acid, and 5 parts of oat extract.

[0011] According to further research, the preparation method of the anti-inflammatory liposome is:

[0012] Step 1: dissolving sodium polyglutamate and hydroxymethyl chitosan in a weight ratio of 2-4:7 in water with a mass multiple of 6-8 times to obtain a water-soluble mixture;

[0013] Step 2: Weigh soybean lecithin into a round-bottom flask, add an aqueous mixture with a mass ratio of 1:3-5, and then perform water bath sonication to obtain anti-inflammatory liposomes.

[0014] According to further research, the temperature of the water bath ultrasound is 50-70°C and the time is 10-20min.

[0015] According to further research, the onion ginger mixed extract is prepared by extracting and purifying onion and ginger in a weight ratio of 6-9:2-4.

[0016] Further research has shown that the preparation method of the onion ginger mixed extract is as follows: the onion and ginger are crushed and added to an extraction tank, an ethanol solution with a mass multiple of 3-5 times is added for extraction for 12-14 hours, and then after reduced pressure compression, methanol and ethyl acetate with mass multiples of 1-3 times and 2-2.8 times are added for purification, and after heating and refluxing, reduced pressure distillation is performed to obtain the onion ginger mixed extract.

[0017] According to further research, the heating reflux temperature is 70-80°C and the time is 6-8h.

[0018] According to further research, the preparation method of the composition is as follows: adding umbilical cord mesenchymal stem cell exosomes to anti-inflammatory liposomes for homogenous mixing at a mixing temperature of 50-60°C, then adding a mixture of hyaluronic acid and water, stirring for a second time, and finally adding onion ginger mixed extract and oat extract for heating and mixing to obtain the composition.

[0019] Further research is conducted on the application of the exosome-containing composition in anti-allergic skin care products.

[0020] Beneficial effects: The main anti-allergic effect of the composition described in the present invention is played by the umbilical cord mesenchymal stem cell exosomes, which are encapsulated by anti-inflammatory liposomes and supplemented with anti-inflammatory substances such as onion ginger mixed extract and oat extract. The anti-allergic effect is significantly enhanced, which can repair the skin barrier, reduce inflammation, and restore the skin to a stable state. DETAILED DESCRIPTION

[0021] Example 1

[0022] The present embodiment provides a composition containing exosomes, comprising the following components in parts by weight: 5 parts of umbilical cord mesenchymal stem cell exosomes, 2 parts of onion ginger mixed extract, 2 parts of anti-inflammatory liposomes, 1 part of hyaluronic acid, and 5 parts of oat extract.

[0023] The preparation method of the anti-inflammatory liposome is:

[0024] Step 1: dissolving sodium polyglutamate and hydroxymethyl chitosan in a weight ratio of 3:7 in water with a mass multiple of 7 to obtain a water-soluble mixture;

[0025] Step 2: Weigh soybean lecithin into a round-bottom flask, add an aqueous mixture with a mass ratio of 1:4, and then perform water bath sonication to obtain anti-inflammatory liposomes.

[0026] The temperature of the water bath ultrasound is 60°C and the time is 15 min.

[0027] The onion and ginger mixed extract is prepared by extracting and purifying onion and ginger in a weight ratio of 8:3.

[0028] The preparation method of the onion-ginger mixed extract is as follows: onion and ginger are crushed and added to an extraction tank, an ethanol solution with a mass multiple of 4 is added for extraction for 13 hours, methanol and ethyl acetate with a mass multiple of 2 and 2.4 respectively are added after decompression and compression for purification, and the onion-ginger mixed extract is obtained by decompression distillation after heating and reflux.

[0029] The heating reflux temperature is 75°C and the time is 7h.

[0030] The preparation method of the composition is as follows: adding umbilical cord mesenchymal stem cell exosomes to anti-inflammatory liposomes for homogenous mixing at a mixing temperature of 55°C, then adding a mixture of hyaluronic acid and water for secondary stirring, and finally adding onion ginger mixed extract and oat extract for heating and mixing to obtain the composition.

[0031] Example 2

[0032] The present embodiment provides a composition containing exosomes, comprising the following components in weight: 4 parts of umbilical cord mesenchymal stem cell exosomes, 1 part of onion ginger mixed extract, 1 part of anti-inflammatory liposomes, 0.5 parts of hyaluronic acid, and 3 parts of oat extract.

[0033] The preparation method of the anti-inflammatory liposome is:

[0034] Step 1: dissolving sodium polyglutamate and hydroxymethyl chitosan in a weight ratio of 2:7 in water with a mass multiple of 6 to obtain a water-soluble mixture;

[0035] Step 2: Weigh soybean lecithin into a round-bottom flask, add an aqueous mixture with a mass ratio of 1:3, and then perform water bath sonication to obtain anti-inflammatory liposomes.

[0036] The temperature of the water bath ultrasound is 50°C and the time is 10 min.

[0037] The onion and ginger mixed extract is prepared by extracting and purifying onion and ginger in a weight ratio of 6:2.

[0038] The preparation method of the onion-ginger mixed extract is as follows: onion and ginger are crushed and added to an extraction tank, an ethanol solution with a mass multiple of 3 is added for extraction for 12 hours, methanol and ethyl acetate with a mass multiple of 1 and 2 are added respectively for purification after reduced pressure compression, and the onion-ginger mixed extract is obtained by reduced pressure distillation after heating and reflux.

[0039] The heating reflux temperature is 70°C and the time is 6h.

[0040] The preparation method of the composition is as follows: adding umbilical cord mesenchymal stem cell exosomes to anti-inflammatory liposomes for homogenous mixing at a mixing temperature of 50°C, then adding a mixture of hyaluronic acid and water for secondary stirring, and finally adding onion ginger mixed extract and oat extract for heating and mixing to obtain the composition.

[0041] Example 3

[0042] The present embodiment provides a composition containing exosomes, comprising the following components in weight: 6 parts of umbilical cord mesenchymal stem cell exosomes, 3 parts of onion ginger mixed extract, 3 parts of anti-inflammatory liposomes, 1.5 parts of hyaluronic acid, and 6 parts of oat extract.

[0043] The preparation method of the anti-inflammatory liposome is:

[0044] Step 1: dissolving sodium polyglutamate and hydroxymethyl chitosan in a weight ratio of 4:7 in water with a mass multiple of 8 times to obtain a water-soluble mixture;

[0045] Step 2: Weigh soybean lecithin into a round-bottom flask, add an aqueous mixture with a mass ratio of 1:5, and then perform water bath sonication to obtain anti-inflammatory liposomes.

[0046] The temperature of the water bath ultrasound is 70°C and the time is 20 min.

[0047] The onion and ginger mixed extract is prepared by extracting and purifying onion and ginger in a weight ratio of 9:4.

[0048] The preparation method of the onion-ginger mixed extract is as follows: onion and ginger are crushed and added to an extraction tank, an ethanol solution with a mass multiple of 5 is added for extraction for 14 hours, methanol and ethyl acetate with a mass multiple of 3 and 2.8 times are added respectively after reduced pressure compression for purification, and the onion-ginger mixed extract is obtained by reduced pressure distillation after heating and reflux.

[0049] The heating reflux temperature is 80°C and the time is 8h.

[0050] The preparation method of the composition is as follows: adding umbilical cord mesenchymal stem cell exosomes to anti-inflammatory liposomes for homogenous mixing at a mixing temperature of 60°C, then adding a mixture of hyaluronic acid and water for secondary stirring, and finally adding onion ginger mixed extract and oat extract for heating and mixing to obtain the composition.

[0051] Comparative Example 1

[0052] The difference between Comparative Example 1 and Example 1 is that no umbilical cord mesenchymal stem cell exosomes are added in Comparative Example 1.

[0053] Comparative Example 2

[0054] The difference between Comparative Example 2 and Example 1 is that the onion ginger mixed extract is not added in Comparative Example 2.

[0055] Comparative Example 3

[0056] The difference between Comparative Example 3 and Example 1 is that no anti-inflammatory liposomes are added in Comparative Example 3.

[0057] Comparative Example 4

[0058] The difference between Comparative Example 4 and Example 1 is that hyaluronic acid is not added in Comparative Example 4.

[0059] Comparative Example 5

[0060] The difference between Comparative Example 5 and Example 1 is that no oat extract is added in Comparative Example 5.

[0061] Comparative Example 6

[0062] The difference between Comparative Example 6 and Example 1 is that the preparation method of the onion ginger mixed extract of Comparative Example 6 is: the onion and ginger are crushed and added to the extraction tank, and an ethanol solution with a mass multiple of 5 times is added for extraction for 13 hours, and then the onion ginger mixed extract is obtained after decompression and compression.

[0063] Comparative Example 7

[0064] The difference between Comparative Example 7 and Example 1 is that onion extract is used in Comparative Example 7 instead of the onion-ginger mixed extract, and the preparation method is the same as that of the onion-ginger mixed extract.

[0065] Comparative Example 8

[0066] The difference between Comparative Example 8 and Example 1 is that the preparation method of the anti-inflammatory liposomes in Comparative Example 8 is different, and the preparation method is: dissolving hydroxymethyl chitosan in water with a mass multiple of 7 times to obtain a water-soluble mixture;

[0067] Step 2: Weigh soybean lecithin into a round-bottom flask, add an aqueous mixture with a mass ratio of 1:4, and then stir to mix to obtain anti-inflammatory liposomes.

[0068] Efficacy verification

[0069] Pentachloronitrobenzene was used to model the right back skin of rats, so that the skin showed symptoms such as swelling, erythema, erosion, etc., and the compositions prepared in the embodiment and the comparative example were applied to the surface respectively. The application period was one month and the frequency was once a day. The skin lesions on the right back of rats in each group were observed with the naked eye, and the right back skin of rats without modeling was set as a control group, and the model was successfully established but no drug was applied as a blank control group, and the HIS and Ig E concentrations in the serum of each sample were detected. The test results are shown in Table 1.

[0070] Table 1

[0071]

[0072] It can be seen from the results in Table 1 that the composition prepared by the present invention has a significant anti-allergic effect, effectively reduces the concentration of HIS and Ig E in rat serum, and significantly reduces allergic inflammation; from Comparative Examples 1-5, it can be seen that the umbilical cord mesenchymal stem cell exosomes added by the present invention are effective anti-allergic components, and other components such as onion ginger mixed extract, anti-inflammatory liposomes, hyaluronic acid, and oat extract play a synergistic role, and the anti-allergic effect of the composition is significantly enhanced compared with not adding any of them; from Comparative Examples 6-7, it can be seen that the onion ginger mixed extract added by the present invention is extracted and purified by onion ginger mixed extraction, and the anti-allergic effect is stronger than that of using a single raw material and other preparation methods; from Comparative Example 8, it can be seen that the anti-allergic liposomes prepared by the present invention have anti-allergic and anti-inflammatory effects while encapsulating exosomes compared with those prepared by other preparation methods, and maintain their effects as much as possible, and the desensitization and repair effect of the prepared composition is greatly improved.

[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing exosomes, characterized in that: The invention comprises the following components in parts by weight: 4-6 parts of umbilical cord mesenchymal stem cell exosomes, 1-3 parts of onion and ginger mixed extract, 1-3 parts of anti-inflammatory liposomes, 0.5-1.5 parts of hyaluronic acid, and 3-6 parts of oat extract; The preparation method of the anti-inflammatory liposome is: Step 1: dissolving sodium polyglutamate and hydroxymethyl chitosan in a weight ratio of 2-4:7 in water with a mass multiple of 6-8 times to obtain a water-soluble mixture; Step 2: Weigh soybean lecithin into a round-bottom flask, add an aqueous mixture with a mass ratio of 1:3-5, and then perform water bath sonication to obtain anti-inflammatory liposomes; The onion ginger mixed extract is prepared by extracting and purifying onion and ginger in a weight ratio of 6-9:2-4; The preparation method of the onion ginger mixed extract is as follows: onion and ginger are crushed and Add to the extraction tank, add ethanol solution with a mass multiple of 3-5 times to extract for 12-14 hours, then reduce pressure and compress, add methanol and ethyl acetate with a mass multiple of 1-3 times and 2-2.8 times respectively to purify, heat and reflux, and perform reduced pressure distillation to obtain an onion ginger mixed extract.

2. The exosome-containing composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 4-5 parts of umbilical cord mesenchymal stem cell exosomes, 2-3 parts of onion and ginger mixed extract, 2-3 parts of anti-inflammatory liposomes, 1-1.5 parts of hyaluronic acid and 4-6 parts of oat extract.

3. The exosome-containing composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 5 parts of umbilical cord mesenchymal stem cell exosomes, 2 parts of onion and ginger mixed extract, 2 parts of anti-inflammatory liposomes, 1 part of hyaluronic acid and 5 parts of oat extract.

4. The exosome-containing composition according to claim 1, characterized in that: The temperature of the water bath ultrasound is 50-70°C and the time is 10-20min.

5. The exosome-containing composition according to claim 1, characterized in that: The heating reflux temperature is 70-80°C and the time is 6-8h.

6. The exosome-containing composition according to any one of claims 1 to 5, characterized in that: The preparation method of the composition is as follows: adding umbilical cord mesenchymal stem cell exosomes to anti-inflammatory liposomes for homogenous mixing at a mixing temperature of 50-60°C, then adding a mixture of hyaluronic acid and water for secondary stirring, and finally adding onion ginger mixed extract and oat extract for heating and mixing to obtain the composition.

7. Use of the exosome-containing composition according to any one of claims 1 to 5 in anti-allergic skin care products.

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