A soil DNA extraction device for investigating the diversity of plant root microbial communities

Abstract

The invention discloses a soil DNA extraction device for investigating the diversity of plant root microbial communities in the technical field of plant root microbial diversity research, comprising: a base and a bracket installed on the base, a turntable is rotatably installed on the top of the base, a mounting frame is provided on the turntable, and a plurality of mounting rings are provided on the mounting frame; a rotating ring and a driving motor are rotatably provided on the mounting ring; a cleaning component is provided on the base, and a sampling tube and a driving mechanism are provided on the bracket; in the present application, the turntable is rotated to make the mounting frame rotate with the mounting ring and the rotating ring, so as to drive a plurality of test tubes inserted in the rotating ring to circulate, move the used test tubes to the cleaning component for cleaning, and move the empty test tubes to the working area, so that a plurality of test tubes are circulated, the number of test tubes required to be used during work is reduced, and the test tubes are cleaned during work, so as to reduce the workload of the staff at a later stage.

Description

Technical Field

[0001] The invention relates to the technical field of plant root microbial diversity research, in particular to a soil DNA extraction device used for investigating the diversity of plant root microbial communities. Background Art

[0002] When investigating the diversity of plant root microbial communities, soil needs to be collected and DNA extraction and analysis is performed on the collected soil. The soil DNA extraction method is to add reagents to the sample soil, extract a clear liquid after centrifugal separation, add reagents to the clear liquid, centrifuge, extract the clear liquid again, and obtain soil DNA after multiple centrifugal extractions. For example, the soil DNA extraction method is disclosed in the public document of a soil DNA extraction kit and a method for extracting soil DNA with publication number CN106011130B;

[0003] However, in the process of DNA extraction, continuous centrifugation and reagent addition are required, resulting in the use of clean test tubes more frequently. Due to the lack of equipment for test tube processing during the extraction process, the test tubes cannot be used again after use (later cleaning is required), so a large number of test tubes are required in the DNA extraction process, resulting in test tube accumulation, which requires later unified processing, increasing the workload in the soil DNA extraction process; if the test tubes are simply rinsed and used, although the number of test tubes used can be reduced, since the test tubes are not cleaned properly and contain residues, the next extraction solution will contain interfering substances, which will affect the accuracy of the plant root microbial community diversity survey. Summary of the invention

[0004] The purpose of the present invention is to provide a soil DNA extraction device for investigating the diversity of plant root microbial communities, so as to solve the problem raised in the above-mentioned background technology that the test tubes cannot be reused after use due to the lack of equipment for handling test tubes during the extraction process, so that a large number of test tubes are required during the DNA extraction process, resulting in test tube accumulation.

[0005] To achieve the above object, the present invention provides the following technical solution: a soil DNA extraction device for investigating the diversity of plant root microbial communities, comprising: a base and a bracket mounted on the base, a turntable rotatably mounted on the top of the base, a mounting frame provided on the turntable, and a plurality of groups of mounting rings distributed in an annular shape provided on the mounting frame;

[0006] The mounting ring is rotatably provided with a rotating ring for placing the test tube and a driving motor for driving the rotating ring to rotate;

[0007] The base is provided with a cleaning assembly for cleaning the test tubes, the bracket is provided with a sampling tube for extracting liquid in the test tubes and a driving mechanism for driving the sampling tubes to rise and fall; the turntable rotates, so that the mounting frame rotates with the mounting ring and the rotating ring, driving a plurality of groups of test tubes inserted in the rotating ring to circulate, moving the used test tubes to the cleaning assembly for cleaning, and moving the empty test tubes to the working area.

[0008] Preferably, the cleaning component comprises a telescopic member mounted on the bracket, a first motor is provided on the moving end of the telescopic member, a rotating rod is provided on the output end of the first motor, a cleaning member is provided on the side wall of the rotating rod, and a water supply component is provided on the base;

[0009] A mounting piece is provided at the connection portion between the mounting frame and the mounting ring, the mounting ring is rotatably mounted on the mounting piece, a second motor for driving the mounting piece to rotate is provided on the mounting piece, and a first collecting plate is provided on the top of the turntable directly below the mounting ring.

[0010] Preferably, a mounting rod is provided on the bracket, a support plate and a third motor for driving the support plate to rotate are provided on the mounting rod, and a second collecting tray is provided on the support plate directly below the rotating rod.

[0011] Preferably, the mounting rod is provided with a pressure ring for being mounted on the rotating rod and a telescopic assembly for driving the pressure ring to rise and fall, and the distance between the axis of the rotating rod and the outer side wall of the cleaning piece is greater than the outer radius of the pressure ring.

[0012] Preferably, a rotating plate and a driving member for driving the rotating plate to rotate are provided on the bracket, and there are several groups of the sampling tubes and the driving mechanisms, and the several groups of the driving mechanisms are distributed in a ring shape on the rotating plate.

[0013] Preferably, a mounting plate is provided on the bracket, a heating element is provided on the side wall of the mounting plate, a mounting tube is provided on the mounting plate and is directly below a sampling tube, a liquid storage tank is provided on the mounting plate, a pump is provided in the liquid storage tank, the outlet of the pump is connected to the side wall of the mounting tube through a conduit, solenoid valves are provided on the conduit and the mounting tube, and a collecting box connected to the bottom end of the mounting tube is provided on the mounting plate.

[0014] Preferably, the mounting tube comprises a tube body mounted on a mounting plate, and a moving tube and a telescopic rod for driving the moving tube to move are slidably disposed on the top of the tube body.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: in the present application, the turntable is rotated to make the mounting frame rotate together with the mounting ring and the rotating ring, driving several groups of test tubes inserted in the rotating ring to rotate in a circular manner, moving the used test tubes to the cleaning assembly for cleaning, and moving the empty test tubes to the working area, so that several groups of test tubes can be recycled, reducing the number of test tubes required for work, and cleaning the test tubes during the work process, reducing the workload of the staff in the later stage, and improving the efficiency of soil DNA extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the present invention;

[0017] Figure 2 This is an enlarged schematic diagram of the structure at A of the present invention;

[0018] Figure 3 This is a schematic diagram of the connection structure between the base and the bracket of the present invention;

[0019] Figure 4 It is an enlarged schematic diagram of the structure at B of the present invention;

[0020] Figure 5 This is a schematic diagram of the connection structure between the rotating plate and the sampling tube of the present invention;

[0021] Figure 6 This is a schematic diagram of the connection structure between the mounting plate and the mounting pipe of the present invention;

[0022] Figure 7 It is an enlarged schematic diagram of the structure at position C of the present invention.

[0023] In the figure: 1. base; 2. bracket; 3. turntable; 4. mounting frame; 5. mounting ring; 51. rotating ring; 52. mounting part; 6. first collecting tray; 7. sampling tube; 8. mounting plate; 9. driving part; 10. rotating plate; 11. telescopic part; 12. driving mechanism; 13. water supply assembly; 14. mounting rod; 15. supporting plate; 16. rotating rod; 17. cleaning part; 18. second collecting tray; 19. pressing ring; 20. mounting tube; 201. tube body; 202. moving tube; 21. liquid storage tank; 22. collecting tank; 23. heating part. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] See also Figure 1 and Figure 3 A soil DNA extraction device for investigating the diversity of plant root microbial communities comprises: a base 1, a bracket 2 is installed on the top of the base 1, and a turntable 3 is rotatably installed on the top of the base 1.

[0027] In this embodiment, as a further optimized solution, please refer to Figure 1 and Figure 3 In order to make the turntable 3 rotate more conveniently, a motor is installed on the base 1, and the output end of the motor is connected to the turntable 3 for driving the turntable 3 to rotate.

[0028] See also Figure 1 , Figure 2 and Figure 3 A mounting frame 4 is provided on the turntable 3, and a plurality of mounting rings 5 ​​are provided on the mounting frame 4. The plurality of mounting rings 5 ​​are distributed in a ring shape on the mounting frame 4; a rotating ring 51 is rotatably provided on the mounting ring 5, and a driving motor is installed on the mounting ring 5, and an output end of the driving motor is connected to the rotating ring 51.

[0029] See also Figure 1 and Figure 3 , a cleaning assembly is provided on the base 1, a driving mechanism 12 (such as an electric telescopic rod) is provided on the bracket 2, and a sampling tube 7 is installed on the moving end of the driving mechanism 12;

[0030] Among them, see Figure 3 and Figure 4 The cleaning component includes a telescopic member 11 (such as an electric telescopic rod) installed on the bracket 2, a first motor is provided on the moving end of the telescopic member 11, a rotating rod 16 is provided on the output end of the first motor, and a cleaning member 17 (such as a sponge block) is provided on the side wall of the rotating rod 16; a water supply component 13 (including a water tank and a water pump installed in the water tank, a pipe is installed on the water outlet of the water pump, and the end of the pipe away from the water pump is directly above a mounting ring 5) is provided on the base 1; a mounting member 52 is provided at the connecting portion between the mounting frame 4 and the mounting ring 5, the mounting ring 5 is rotatably mounted on the mounting member 52, a second motor is provided on the mounting member 52, and the output end of the second motor is connected to the mounting ring 5 (the second motor drives the mounting ring 5 to rotate, so that the mounting ring 5 flips over), and a plurality of groups of first collection trays 6 are provided on the top of the turntable 3, the number of the first collection trays 6 is the same as the number of the mounting rings 5, and the plurality of groups of first collection trays 6 are respectively directly below the plurality of groups of mounting rings 5, and correspond one to one.

[0031] It should be noted that the working method of the extraction device is to insert a test tube into the rotating ring 51, then put the soil sample into a test tube and add the reagent, then drive the motor to rotate the rotating ring 51 here, and drive the test tube to rotate and centrifuge, and after the centrifugation is completed; the driving mechanism 12 moves the sampling tube 7 downward, and after the clear liquid in the test tube is sucked out, the driving mechanism 12 moves the sampling tube 7 upward; then the turntable 3 rotates to remove the used test tube, and moves the empty test tube to the bottom of the sampling tube 7, and then the driving mechanism 12 moves the sampling tube 7 downward, and after the clear liquid in the sampling tube 7 is discharged into the empty test tube, the driving mechanism 12 moves the sampling tube 7 upward, and then the additive liquid is added to the test tube containing the clear liquid; then the driving motor drives the rotating ring 51 and the test tube 7 to rotate. The tube rotates for centrifugal treatment. After completion, the clear liquid is taken out using the sampling tube 7, the turntable 3 rotates, and the operation is repeated until the soil DNA is extracted; when the used test tube rotates to the cleaning component, the water supply component 13 injects water into the test tube, and then the telescopic member 11 works, driving the rotating rod 16 downward to be inserted into the test tube, and then the first motor works to rotate the rotating rod 16 and the cleaning member 17, and the test tube is cleaned using the cleaning member 17. After completion, the telescopic member 11 drives the rotating rod 16 upward; then the second motor drives the mounting ring 5 to rotate, so that the test tube is turned over, and the dirt in the test tube is poured into the first collecting plate 6; the second motor drives the test tube to reverse and reset, and the cleaning component can repeatedly clean a test tube multiple times according to the setting to ensure that it is clean.

[0032] In this embodiment, as a further optimized solution, please refer to Figure 4 A mounting rod 14 is provided on the bracket 2, and a third motor is provided on the mounting rod 14. A support plate 15 is installed on the output end of the third motor, and a second collecting tray 18 is installed on the top of the support plate 15. The second collecting tray 18 is directly below the rotating rod 16; after the cleaning component completes cleaning and the telescopic member 11 brings the rotating rod 16 back to its original position, the third motor works to rotate the support plate 15, and rotates the second collecting tray 18 to directly below the rotating rod 16, so as to collect the sewage falling from the rotating rod 16 and the cleaning member 17.

[0033] In this embodiment, as a further optimized solution, please refer to Figure 4 A telescopic assembly (such as an electric telescopic rod) is provided on the mounting rod 14, and a pressure ring 19 is provided on the movable end of the telescopic assembly. The pressure ring 19 is sleeved on the rotating rod 16, and the distance between the axis of the rotating rod 16 and the outer side wall of the cleaning member 17 is greater than the outer radius of the pressure ring 19; when the second collecting tray 18 moves to the bottom of the rotating rod 16, the telescopic assembly works to move the pressure ring 19 downward to squeeze the cleaning member 17 to squeeze out the sewage adsorbed in the cleaning member 17, thereby ensuring the cleanliness of the cleaning member 17 and improving the effect of the cleaning member 17 when used next time.

[0034] Example 2

[0035] As an optimized solution, see Figure 1 , Figure 3 and Figure 5 A driving member 9 (such as a motor) is provided on the bracket 2, and a rotating plate 10 is installed on the output end of the driving member 9; there are several groups of sampling tubes 7 and driving mechanisms 12, and the several groups of driving mechanisms 12 are distributed in a ring shape on the rotating plate 10; after a sampling tube 7 discharges the absorbed clear liquid into the next test tube, the driving member 9 drives the rotating plate 10 to rotate, so that the driving mechanism 12 and the sampling tube 7 rotate, and the used sampling tube 7 is removed from the working area, and the unused sampling tube 7 is moved to the working area, so as to avoid the used sampling tube 7 being used again and affecting the accuracy of DNA extraction.

[0036] In this embodiment, as a further optimized solution, please refer to Figure 1 , Figure 3 , Figure 6 and Figure 7 , a mounting plate 8 is provided on the bracket 2, and a heating element 23 (electric heating element, such as a heating plate) is provided on the side wall of the mounting plate 8; a mounting tube 20 is provided on the mounting plate 8, and the mounting tube 20 is located directly below a sampling tube 7 (the used sampling tube 7 will be moved directly above the mounting tube 20), a liquid storage tank 21 is provided on the mounting plate 8 (the liquid storage tank 21 is filled with cleaning liquid), a pump is provided in the liquid storage tank 21, and the outlet of the pump is connected to the side wall of the mounting tube 20 through a conduit, and solenoid valves are provided on the conduit and the mounting tube 20, and a collecting box 22 connected to the bottom end of the mounting tube 20 is provided on the mounting plate 8; the mounting tube 20 includes a tube body 201 installed on the mounting plate 8, and a moving tube 202 is slidably provided on the top of the tube body 201 (the moving tube 202 is slidably sleeved on the outer wall of the tube body 201 or slidably inserted into the inner side of the tube body 201), and the tube body 201 A telescopic rod (such as an electric telescopic rod) is installed on it, and the movable end of the telescopic rod is connected to the movable tube 202; when the used sampling tube 7 moves to the top of the mounting tube 20, the telescopic rod works to move the movable tube 202 upward and insert it into the sampling tube 7; then the solenoid valve on the catheter is opened, and the pump works to input the cleaning liquid inside the liquid storage tank 21 from the catheter into the sampling tube 7 to flush the inside of the sampling tube 7; then the solenoid valve on the catheter is closed, the pump stops working, and the solenoid valve on the mounting tube 20 is opened, so that the used cleaning liquid in the sampling tube 7 enters the interior of the collecting box 22 through the mounting tube 20, and the used sampling tube 7 is cleaned, so that the sampling tube 7 can be reused, which is used to reduce the number of sampling tubes 7 used in work; after flushing is completed, the heating element 23 works to dry the flushed sampling tube 7.

[0037] It should be noted that, according to the cleaning requirements, a single sampling tube 7 can be rinsed multiple times to ensure that the sampling tube 7 is clean.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A soil DNA extraction device for investigating the diversity of plant root microbial communities, comprising: A base (1) and a bracket (2) mounted on the base (1), characterized in that a turntable (3) is rotatably mounted on the top of the base (1), a mounting frame (4) is provided on the turntable (3), and a plurality of groups of mounting rings (5) distributed in an annular shape are provided on the mounting frame (4); The mounting ring (5) is rotatably provided with a rotating ring (51) for placing a test tube and a driving motor for driving the rotating ring (51) to rotate; The base (1) is provided with a cleaning assembly for cleaning the test tubes, and the support (2) is provided with a sampling tube (7) for extracting liquid in the test tubes and a driving mechanism (12) for driving the sampling tube (7) to rise and fall; the turntable (3) rotates, so that the mounting frame (4) drives the mounting ring (5) and the rotating ring (51) to rotate together, driving a plurality of groups of test tubes inserted in the rotating ring (51) to rotate cyclically, moving the used test tubes to the cleaning assembly for cleaning, and moving the empty test tubes to the working area; The cleaning component comprises a telescopic member (11) mounted on a bracket (2); a first motor is provided on a movable end of the telescopic member (11); a rotating rod (16) is provided on an output end of the first motor; a cleaning member (17) is provided on a side wall of the rotating rod (16); and a water supply component (13) is provided on the base (1); Wherein, a mounting member (52) is provided at the connection portion between the mounting frame (4) and the mounting ring (5); the mounting ring (5) is rotatably mounted on the mounting member (52); a second motor for driving the mounting member (52) to rotate is provided on the mounting member (52); and a first collecting plate (6) is provided on the top of the rotating disk (3) and is located directly below the mounting ring (5); The bracket (2) is provided with a mounting rod (14), the mounting rod (14) is provided with a support plate (15) and a third motor for driving the support plate (15) to rotate, and the support plate (15) is provided with a second collecting plate (18) directly below the rotating rod (16); The mounting rod (14) is provided with a pressure ring (19) for being sleeved on the rotating rod (16) and a telescopic assembly for driving the pressure ring (19) to rise and fall. The distance between the axis of the rotating rod (16) and the outer side wall of the cleaning member (17) is greater than the outer radius of the pressure ring (19).

2. A soil DNA extraction device for investigating the diversity of plant root microbial communities according to claim 1, characterized in that: The support (2) is provided with a rotating plate (10) and a driving member (9) for driving the rotating plate (10) to rotate. The sampling tube (7) and the driving mechanism (12) are provided in a plurality of groups. The plurality of driving mechanisms (12) are distributed in a ring shape on the rotating plate (10).

3. A soil DNA extraction device for investigating the diversity of plant root microbial communities according to claim 2, characterized in that: The bracket (2) is provided with a mounting plate (8), a heating element (23) is provided on the side wall of the mounting plate (8), a mounting tube (20) is provided on the mounting plate (8) and is located directly below a sampling tube (7), a liquid storage tank (21) is provided on the mounting plate (8), a pump is provided in the liquid storage tank (21), an outlet of the pump is connected to the side wall of the mounting tube (20) through a conduit, and solenoid valves are provided on the conduit and the mounting tube (20), and a collecting box (22) is provided on the mounting plate (8) and is connected to the bottom end of the mounting tube (20).

4. A soil DNA extraction device for investigating the diversity of plant root microbial communities according to claim 3, characterized in that: The mounting tube (20) comprises a tube body (201) mounted on a mounting plate (8), and a moving tube (202) and a telescopic rod for driving the moving tube (202) to move are slidably provided on the top of the tube body (201).

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