A Chinese medicinal probiotic composition for improving oral bacterial flora and its preparation method and application

Abstract

The present invention relates to a Chinese medicine probiotic composition for improving oral bacteria, and a preparation method and application thereof, and belongs to the technical field of probiotic application. The Chinese medicine probiotic composition of the present invention is formed by fermenting a Chinese medicine extract with a probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition; the probiotic composition is composed of the following raw materials: Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius; the Chinese medicine composition is composed of the following raw materials: Jerusalem artichoke, Artemisia argyi, cinnamon bark, Evergreen, tea tree leaf, Magnolia officinalis, corn silk, clove, Ginkgo biloba, licorice, loquat leaf and Polygonatum odoratum. The Chinese medicine probiotic composition of the present invention converts the Chinese medicine extract into a small molecule substance that is easily absorbed by fermentation, and then improves the oral bacteria group through the generated effective components, maintains oral health, and can effectively prevent and treat problems such as dental diseases and bad breath.

Description

Technical Field

[0001] The invention belongs to the technical field of probiotic application, and in particular relates to a traditional Chinese medicine probiotic composition for improving oral bacteria population, and a preparation method and application thereof. Background Art

[0002] In recent years, as living standards continue to improve, people's awareness of hygiene has also been increasing. At the same time, bad living habits, such as irregular diet, staying up late, little exercise, long working hours, and high pressure, have also led to impaired human immunity, and problems such as swollen gums and oral ulcers have also increased.

[0003] The oral cavity is the starting point of the digestive tract. It is the first to come into contact with probiotics and also has its own unique ecosystem. A large number of microorganisms of various types live in the normal human oral cavity, mainly bacteria, collectively referred to as oral flora. Bacteria can be divided into anaerobic bacteria, facultative anaerobic bacteria and aerobic bacteria according to their oxygen requirements. The oral flora is mostly composed of anaerobic bacteria. The relationship between the oral flora and the human body is intricate and is an indispensable part of the human body. Under normal circumstances, the various oral bacteria and the host are interdependent and mutually restricted, maintaining a dynamic ecological balance. Once affected by changes in the host and the external environment, the equilibrium state will be broken, resulting in oral flora imbalance. In recent years, more and more studies have focused on the role of probiotics in the balance of oral flora, and the research and development of oral probiotic products has very good social and economic benefits.

[0004] Probiotics are a type of active microorganisms that are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host. They promote nutrient absorption and maintain intestinal health by regulating the host mucosal and systemic immune functions or by regulating the balance of intestinal flora, thereby producing single microorganisms or mixed microorganisms with clear composition that are beneficial to health. They mainly exist in the gastrointestinal tract, oral cavity and reproductive system of the host, and can improve the microecological environment in the host, thereby promoting the host's physical health.

[0005] At present, probiotics are widely used in the fields of medical treatment, food, etc. For example, patent CN 112754987A discloses an oral care composition containing probiotics and its application, using lac resin sodium salt-carboxymethyl chitosan as a wall material to form a package for probiotics, and adding probiotics to toothpaste in the form of microcapsules. The toothpaste provided adds a variety of probiotics that have inhibitory effects on oral pathogens, which can secrete a variety of antibacterial substances and maintain the microecological balance in the oral cavity, and has a good therapeutic effect on gingivitis and halitosis. However, when this existing probiotic composition is applied to the oral cavity, it is mostly in the form of toothpaste, which is poor for the user's experience and is not convenient for frequent use in daily life. Therefore, the therapeutic effect for oral problems is poor.

[0006] As a traditional medicine, Chinese medicine has significantly promoted human survival and reproduction through disease prevention and treatment. However, the bioactivity and bioavailability of most chemical substances in Chinese medicine are low, while oral probiotics can metabolize Chinese medicine chemical substances, and the metabolites produced have high bioactivity and bioavailability. Chinese medicine chemicals can also effectively promote the growth of oral probiotics, increase the survival rate of live bacteria entering the oral cavity, and regulate the composition of oral microbial flora to restore its homeostasis, thereby better improving human health. Therefore, it is of great significance to develop a Chinese medicine probiotic composition by combining oral probiotics with Chinese medicine compound compositions, which can be used to improve oral bacterial flora. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a Chinese medicine probiotic composition for improving oral bacterial flora, and its preparation method and application. The content of active ingredients of Chinese medicine can be increased by probiotic fermentation of Chinese medicine, and the extracellular enzymes produced by the probiotics, such as cellulase, hemicellulase and pectinase, can rupture the cells of Chinese medicine and expose the active ingredients, and degrade macromolecular substances into small molecules that are easier to absorb. At the same time, it has the advantages of enhancing drug efficacy, expanding the indications of Chinese medicine, reducing the toxicity of Chinese medicine, regulating intestinal flora, and improving utilization.

[0008] The first object of the present invention is to provide a Chinese medicine probiotic composition, which is obtained by fermenting a Chinese medicine extract with a probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition;

[0009] The probiotic composition is composed of the following raw materials: Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius;

[0010] The traditional Chinese medicine composition is composed of the following raw materials: Jerusalem artichoke, Artemisia argyi, cinnamon bark, evergreen, tea tree leaf, magnolia bark, corn silk, clove, ginkgo fruit, liquorice, loquat leaf and polygonatum odoratum.

[0011] In one embodiment of the present invention, the probiotic composition is composed of the following raw materials by weight: 5-10 parts of Lactobacillus rhamnosus, 5-10 parts of Lactobacillus reuteri, 5-10 parts of Lactobacillus bulgaricus, 8-20 parts of Bacillus subtilis, 2-6 parts of Bifidobacterium, 2-6 parts of Lactobacillus acidophilus, 2-6 parts of Lactobacillus plantarum and 8-20 parts of Lactobacillus salivarius;

[0012] The Chinese medicine composition consists of the following raw materials by weight: 12-20 parts of Jerusalem artichoke, 12-20 parts of Artemisia argyi, 12-20 parts of cinnamon bark, 12-20 parts of Sijiqing, 8-16 parts of tea leaves, 8-16 parts of Magnolia officinalis, 8-16 parts of corn silk, 8-16 parts of cloves, 5-10 parts of ginkgo nuts, 5-10 parts of liquorice, 5-10 parts of loquat leaves and 5-10 parts of Polygonatum odoratum.

[0013] In one embodiment of the present invention, the viable count of Lactobacillus rhamnosus in the probiotic composition is at least 1×10 9 CFU / g, the number of viable Lactobacillus reuteri is at least 1×10 9 CFU / g, the number of viable Lactobacillus bulgaricus is at least 1×10 9 CFU / g, the number of viable Bacillus subtilis is at least 1×10 9 CFU / g, the number of viable bifidobacteria is at least 1×10 9 CFU / g, the number of viable Lactobacillus acidophilus is at least 1×10 9 CFU / g, the number of viable Lactobacillus plantarum is at least 1×10 9 CFU / g, the number of viable Lactobacillus salivarius is at least 1×10 9 CFU / g.

[0014] The second object of the present invention is to provide a method for preparing the Chinese medicine probiotic composition, comprising the following steps:

[0015] S1. Grind Jerusalem artichoke, Artemisia argyi, cinnamon bark, evergreen, tea tree leaves, Magnolia officinalis, corn silk, clove, ginkgo fruit, licorice, loquat leaf and polygonatum odoratum, mix, add 10-15 times the weight of the raw materials in water, soak, extract with water, and sterilize to obtain a Chinese medicine extract;

[0016] S2. Activate Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius respectively with culture medium, and inoculate them into the Chinese medicine extract described in S1 at a volume percentage of 1%-5%, and obtain the Chinese medicine probiotic composition through fermentation and sterilization.

[0017] In one embodiment of the present invention, in S1, the mesh size after pulverization is 100-150 mesh.

[0018] In one embodiment of the present invention, in S1, the water extraction temperature is 100°C-110°C, and the time is 1h-2h.

[0019] In one embodiment of the present invention, in S2, the culture medium is selected from MRS medium or LB medium.

[0020] In one embodiment of the present invention, in S2, the fermentation temperature is 20°C-40°C, the fermentation time is 2-4 days, and the pH is 3.5-4.2.

[0021] In one embodiment of the present invention, the sterilization temperature is independently 100°C-110°C, and the sterilization time is independently 15min-30min.

[0022] The third object of the present invention is to provide a use of the Chinese medicinal probiotic composition in preparing a product having the function of improving oral bacterial flora.

[0023] The technical solution of the present invention has the following advantages over the prior art:

[0024] (1) The Qi Ai in the Chinese herbal probiotic composition of the present invention can bind to the iron ions on which harmful bacteria depend for survival, thereby blocking their nutrient source and reducing their absorption of iron, an essential element, to achieve the purpose of inhibiting the growth of harmful bacteria; Ginkgo biloba has the effect of nourishing the spleen and stomach, can replenish the body's nutrients, help improve digestive function, and enhance the body's immunity; Polygonatum odoratum is sweet, fat-rich, soft and edible, nourishes yin and qi, is nourishing but not greasy, and is neither cold nor dry. Therefore, it has the functions of nourishing the five internal organs, nourishing qi and blood, nourishing and moisturizing, and removing wind and heat.

[0025] (2) The Chinese herbal probiotic composition of the present invention uses Jerusalem artichoke, Artemisia argyi, cinnamon bark and Polygonatum odoratum in combination, and shows a relatively obvious synergistic promotion effect in inhibiting the growth of Streptococcus mutans and alleviating dental plaque and bad breath problems. In particular, the oligofructose in Jerusalem artichoke and the cinnamaldehyde in cinnamon bark have a synergistic antibacterial effect, and the antibacterial and bactericidal effect is stronger after combination. After the syringin and eugenol in clove extract, the long-stalked holly glycoside in evergreen and cinnamon bark are combined, their antibacterial and bactericidal effects are stronger, and they have a significant inhibitory effect on harmful bacteria in the oral cavity, which can better protect oral health. Magnolia bark phenol in Magnolia officinalis has a strong inhibitory effect on harmful bacteria in the oral cavity; tea leaves are rich in monounsaturated fatty acids, vitamin E, squalene, flavonoids, carotenoids and other nutrients. After the combination of Magnolia officinalis and tea leaves, it not only has a significant inhibitory effect on harmful bacteria in the oral cavity, but also can inhibit the adhesion of harmful bacteria in the oral cavity, effectively reduce dental plaque and bad breath rebound, thereby playing a role in protecting oral health.

[0026] (3) The Bacillus subtilis and Lactobacillus salivarius in the Chinese medicine probiotic composition of the present invention are bacteria with large oxygen consumption. They can consume a large amount of oxygen in the intestine, making the intestinal environment tend to be anaerobic, which is more conducive to the colonization and proliferation of anaerobic Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bifidobacterium and Lactobacillus acidophilus. Lactobacillus rhamnosus is acid-resistant, bile-resistant, and has strong intestinal colonization ability, which can reduce the number of harmful bacteria in the intestine, improve the intestinal environment, and reduce intestinal toxicity factors. Lactobacillus reuteri is acid-resistant and bile-resistant, and can produce short-chain fatty acids, thereby promoting the proliferation of intestinal epithelial cells and facilitating the repair of intestinal mucosa; it can produce reuterin to inhibit the growth of harmful bacteria. Lactobacillus bulgaricus can ferment lactose, and the galactose and glucose produced by fermentation are not only easily absorbed, but also can promote the emptying of gastric contents, reduce excessive gastric acid secretion, improve the utilization rate of calcium, phosphorus and iron, and inhibit harmful bacteria in the intestine. Bifidobacteria can inhibit the growth of harmful bacteria in the intestine, reduce toxins, and provide a good growth environment for beneficial bacteria in the intestine, synthesize multiple vitamins, and improve the body's immune ability. Bifidobacteria interact with gastrointestinal mucosal epithelial cells through teichoic acid, closely bind to them, and occupy the surface of the gastrointestinal mucosa together with other anaerobic bacteria to form a biological barrier.

[0027] (4) The Chinese herbal probiotic composition of the present invention transforms the Chinese herbal extract into easily absorbed small molecules through fermentation, and then improves the oral bacterial flora through the produced effective components, maintains oral health, and can effectively prevent and treat dental diseases and bad breath. DETAILED DESCRIPTION

[0028] The present invention is further described below in conjunction with specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0029] In the present invention, unless otherwise specified, the deposit number of Lactobacillus rhamnosus is CGMCC No.15027; the deposit number of Lactobacillus reuteri is CGMCC No.15703; the deposit number of Lactobacillus bulgaricus is ATCC 7517; and the deposit number of Bifidobacterium is CGMCC No.15708.

[0030] In the present invention, unless otherwise specified, the viable count of Lactobacillus rhamnosus in the examples is about 1×10 10 CFU / g, the number of viable Lactobacillus reuteri is about 1×10 10 CFU / g, the number of viable Lactobacillus bulgaricus is about 1×10 10 CFU / g, the number of viable bacteria of Bacillus subtilis is about 1×10 10 CFU / g, the number of viable bifidobacteria is about 1×10 11CFU / g, the number of viable Lactobacillus acidophilus is about 1×10 11 CFU / g, the number of viable Lactobacillus plantarum is about 1×10 11 CFU / g, the number of viable Lactobacillus salivarius is about 1×10 11 CFU / g.

[0031] Example 1

[0032] The Chinese medicine probiotic composition of the present invention is obtained by fermenting a Chinese medicine extract with the probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition;

[0033] The probiotic composition is composed of the following raw materials by weight: 8 parts of Lactobacillus rhamnosus, 8 parts of Lactobacillus reuteri, 8 parts of Lactobacillus bulgaricus, 14 parts of Bacillus subtilis, 4 parts of Bifidobacterium, 4 parts of Lactobacillus acidophilus, 4 parts of Lactobacillus plantarum and 14 parts of Lactobacillus salivarius;

[0034] The Chinese medicine composition is composed of the following raw materials by weight: 16 parts of Jerusalem artichoke, 16 parts of Artemisia argyi, 16 parts of cinnamon bark, 16 parts of Evergreen, 12 parts of tea leaves, 12 parts of Magnolia officinalis, 12 parts of corn silk, 12 parts of cloves, 8 parts of ginkgo nuts, 8 parts of liquorice, 8 parts of loquat leaves and 8 parts of Polygonatum odoratum.

[0035] The preparation specifically comprises the following steps:

[0036] S1. Grind Jerusalem artichoke, Artemisia argyi, cinnamon bark, Evergreen, tea leaves, Magnolia officinalis, corn silk, cloves, ginkgo, licorice, loquat leaves and Polygonatum odoratum to 100-150 mesh, mix, add 13 times the weight of the raw materials in water, soak for 40 minutes, extract at 105°C for 1.5 hours, and sterilize at 105°C for 23 minutes to obtain a Chinese medicine extract;

[0037] S2. Use LB medium to activate Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius respectively, and inoculate them into the traditional Chinese medicine extract of S1 at a volume percentage of 3%, adjust the pH to 3.8, ferment at 30°C for 3 days, and sterilize at 105°C for 23 minutes to obtain a traditional Chinese medicine probiotic composition.

[0038] Example 2

[0039] The Chinese medicine probiotic composition of the present invention is obtained by fermenting a Chinese medicine extract with the probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition;

[0040] The probiotic composition is composed of the following raw materials by weight: 5 parts of Lactobacillus rhamnosus, 5 parts of Lactobacillus reuteri, 10 parts of Lactobacillus bulgaricus, 20 parts of Bacillus subtilis, 2 parts of Bifidobacterium, 2 parts of Lactobacillus acidophilus, 6 parts of Lactobacillus plantarum and 20 parts of Lactobacillus salivarius;

[0041] The Chinese medicine composition is composed of the following raw materials by weight: 12 parts of Jerusalem artichoke, 12 parts of Artemisia argyi, 20 parts of cinnamon bark, 20 parts of Evergreen, 8 parts of tea leaves, 8 parts of Magnolia officinalis, 16 parts of corn silk, 16 parts of cloves, 5 parts of ginkgo nuts, 5 parts of liquorice, 10 parts of loquat leaves and 10 parts of Polygonatum odoratum.

[0042] The preparation specifically comprises the following steps:

[0043] S1. Grind Jerusalem artichoke, Artemisia argyi, cinnamon bark, evergreen, tea leaves, Magnolia officinalis, corn silk, clove, ginkgo fruit, licorice, loquat leaves and polygonatum to 100-150 mesh, add 15 times the weight of the raw materials in water after mixing, soak for 20 minutes, extract at 100°C for 2 hours, and sterilize at 100°C for 30 minutes to obtain a Chinese medicine extract;

[0044] S2. Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius were activated with LB culture medium respectively, and inoculated into the traditional Chinese medicine extract of S1 at a volume percentage of 1%, the pH was adjusted to 3.5, fermented at 40°C for 2 days, and sterilized at 100°C for 30 minutes to obtain a traditional Chinese medicine probiotic composition.

[0045] Example 3

[0046] The Chinese medicine probiotic composition of the present invention is obtained by fermenting a Chinese medicine extract with the probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition;

[0047] The probiotic composition is composed of the following raw materials by weight: 10 parts of Lactobacillus rhamnosus, 10 parts of Lactobacillus reuteri, 5 parts of Lactobacillus bulgaricus, 8 parts of Bacillus subtilis, 6 parts of Bifidobacterium, 6 parts of Lactobacillus acidophilus, 2 parts of Lactobacillus plantarum and 8 parts of Lactobacillus salivarius;

[0048] The Chinese medicine composition is composed of the following raw materials by weight: 20 parts of Jerusalem artichoke, 20 parts of Artemisia argyi, 12 parts of cinnamon bark, 12 parts of Evergreen, 16 parts of tea leaves, 16 parts of Magnolia officinalis, 8 parts of corn silk, 8 parts of cloves, 10 parts of ginkgo nuts, 10 parts of liquorice, 5 parts of loquat leaves and 5 parts of Polygonatum odoratum.

[0049] The preparation specifically comprises the following steps:

[0050] S1. Grind Jerusalem artichoke, Artemisia argyi, cinnamon bark, Evergreen, tea leaves, Magnolia officinalis, corn silk, cloves, ginkgo, licorice, loquat leaves and Polygonatum odoratum to 100-150 mesh, mix, add 10 times the weight of the raw materials in water, soak for 60 minutes, extract at 110° C. for 1 hour, and sterilize at 110° C. for 15 minutes to obtain a Chinese medicine extract;

[0051] S2. Use LB medium to activate Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius respectively, and inoculate them into the traditional Chinese medicine extract of S1 at a volume percentage of 5%, adjust the pH to 4.2, ferment at 20°C for 4 days, and sterilize at 110°C for 15 minutes to obtain a traditional Chinese medicine probiotic composition.

[0052] Comparative Example 1

[0053] The same as Example 1, except that no Magnolia officinalis was added.

[0054] Comparative Example 2

[0055] The method is basically the same as Example 1, except that no Magnolia bark and tea leaves are added.

[0056] Comparative Example 3

[0057] The method is basically the same as Example 1, except that cinnamon is not added.

[0058] Comparative Example 4

[0059] The method is basically the same as Example 1, except that Bacillus subtilis is not added.

[0060] Comparative Example 5

[0061] The method is basically the same as Example 1, except that Lactobacillus rhamnosus is not added.

[0062] Comparative Example 6

[0063] The method is basically the same as Example 1, except that no bifidobacterium is added.

[0064] Comparative Example 7

[0065] The method is basically the same as Example 1, except that Lactobacillus rhamnosus and Bifidobacterium are not added.

[0066] Comparative Example 8

[0067] Basically the same as Example 1, except that: Lactobacillus bulgaricus.

[0068] Test Example 1

[0069] The Chinese medicinal probiotic compositions of Examples 1-3 and Comparative Examples 1-8 were tested for thermal stability and long-term stability. The test results are shown in Table 1-2 below:

[0070] Table 1

[0071]

[0072]

[0073] Table 2

[0074]

[0075] It can be seen from Tables 1-2 that the Chinese medicinal probiotic composition of the embodiment has excellent thermal stability and long-term stability.

[0076] Test Example 2

[0077] The antibacterial properties of the Chinese herbal probiotic compositions of Examples 1-3 and Comparative Examples 1-8 were detected by diffusion method; the test steps were as follows: 5 mg of each of the above-mentioned groups of sample Chinese herbal probiotic compositions were taken and diluted with sterile deionized water at a ratio of 1:5 to obtain each sample test solution; Streptococcus mutans (ATCC 25175), Actinobacillus actinomycetemcomitans (ATCC 29523), Porphyromonas gingivalis (ATCC33277) and Fusobacterium nucleatum (ATCC 25586) were added to the culture medium (200 mL of 5% tryptic soy broth) to prepare a concentration of 10 6 CFU / mL of bacterial culture fluid. The above-mentioned bacterial culture fluid was evenly cross-smeared on the surface of the culture medium (tryptic soy broth culture medium) of the culture dish, and the culture dish was placed in a constant temperature incubator at 37°C for 24h, and 22 culture dishes of each bacteria were used (divided into 11 groups on average, 2 in each group, corresponding to Examples 1-3 and Comparative Examples 1-8, respectively). The above-mentioned Chinese medicine probiotic composition sample test solution was dripped on a round filter paper with a capillary, and symmetrically attached to the central area on the corresponding culture medium, and the culture dish was placed in a constant temperature incubator at 37°C for 24h, and the diameter (mm) of the circular antibacterial area in each group of culture dishes was measured after 24h and the average value was taken. The results are shown in Table 3 below.

[0078] Table 3

[0079] Sample Streptococcus mutans Actinobacillus actinomycetemcomitans Porphyromonas gingivalis Fusobacterium nucleatum Example 1 66.89 62.94 79.62 63.51 Example 2 66.35 62.68 79.46 63.29 Example 3 66.56 62.76 79.31 63.05 Comparative Example 1 54.36 50.19 66.28 50.34 Comparative Example 2 28.63 24.43 39.94 24.03 Comparative Example 3 56.49 52.76 68.43 52.61 Comparative Example 4 53.61 49.62 65.39 50.08 Comparative Example 5 56.48 52.64 67.16 52.63 Comparative Example 6 55.37 50.96 66.30 51.06 Comparative Example 7 25.47 21.36 39.16 22.35 Comparative Example 8 50.34 46.97 63.59 47.69

[0080] It can be seen from Table 3 that the Chinese medicinal probiotic composition prepared in the embodiment of the present invention has a good inhibitory effect on various pathogenic bacteria in the oral cavity by adding probiotics.

[0081] Obviously, the above embodiments are merely examples for clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the protection scope of the invention.

Claims

1. A Chinese medicinal probiotic composition, characterized in that: The Chinese medicine extract is fermented by the probiotic composition, and the Chinese medicine extract is extracted from the Chinese medicine composition; The probiotic composition is composed of the following raw materials by weight: 5-10 parts of Lactobacillus rhamnosus, 5-10 parts of Lactobacillus reuteri, 5-10 parts of Lactobacillus bulgaricus, 8-20 parts of Bacillus subtilis, 2-6 parts of Bifidobacterium, 2-6 parts of Lactobacillus acidophilus, 2-6 parts of Lactobacillus plantarum and 8-20 parts of Lactobacillus salivarius; The Chinese medicine composition consists of the following raw materials by weight: 12-20 parts of Jerusalem artichoke, 12-20 parts of Artemisia argyi, 12-20 parts of cinnamon bark, 12-20 parts of Sijiqing, 8-16 parts of tea leaves, 8-16 parts of Magnolia officinalis, 8-16 parts of corn silk, 8-16 parts of cloves, 5-10 parts of ginkgo nuts, 5-10 parts of liquorice, 5-10 parts of loquat leaves and 5-10 parts of Polygonatum odoratum.

2. The Chinese medicinal probiotic composition according to claim 1, characterized in that: The number of viable Lactobacillus rhamnosus in the probiotic composition is at least 1×10 9 CFU / g, the number of viable Lactobacillus reuteri is at least 1×10 9 CFU / g, the number of viable Lactobacillus bulgaricus is at least 1×10 9 CFU / g, the number of viable Bacillus subtilis is at least 1×10 9 CFU / g, the number of viable bifidobacteria is at least 1×10 9 CFU / g, the number of viable Lactobacillus acidophilus is at least 1×10 9 CFU / g, the number of viable Lactobacillus plantarum is at least 1×10 9 CFU / g, the number of viable Lactobacillus salivarius is at least 1×10 9 CFU / g.

3. A method for preparing the Chinese medicinal probiotic composition according to any one of claims 1 to 2, characterized in that: The following steps are included: S1. Grind Jerusalem artichoke, Artemisia argyi, cinnamon bark, evergreen, tea tree leaves, Magnolia officinalis, corn silk, clove, ginkgo fruit, licorice, loquat leaf and polygonatum odoratum, mix, add 10-15 times the weight of the raw materials in water, soak, extract with water, and sterilize to obtain a Chinese medicine extract; S2. Activate Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus bulgaricus, Bacillus subtilis, Bifidobacterium, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus salivarius respectively with culture medium, and inoculate them into the Chinese medicine extract described in S1 at a volume percentage of 1%-5%, and obtain the Chinese medicine probiotic composition through fermentation and sterilization.

4. The method for preparing the Chinese medicinal probiotic composition according to claim 3, characterized in that: In S1, the mesh size after pulverization is 100-150 mesh.

5. The method for preparing the Chinese medicinal probiotic composition according to claim 3, characterized in that: In S1, the water extraction temperature is 100°C-110°C, and the time is 1h-2h.

6. The method for preparing the Chinese medicinal probiotic composition according to claim 3, characterized in that: In S2, the culture medium is selected from MRS medium or LB medium.

7. The method for preparing the Chinese medicinal probiotic composition according to claim 3, characterized in that: In S2, the fermentation temperature is 20°C-40°C, the fermentation time is 2-4 days, and the pH is 3.5-4.

2.

8. The method for preparing the Chinese medicinal probiotic composition according to claim 3, characterized in that: The sterilization temperature is independently 100°C-110°C, and the sterilization time is independently 15min-30min.

9. Use of the Chinese medicinal probiotic composition according to any one of claims 1 to 2 in preparing a product having the effect of improving oral bacterial flora.

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