A highly effective and highly permeable double-layer mask containing small molecule essence ingredients

Abstract

The present application provides a high-efficiency and high-penetration double-layer mask containing small molecule essence ingredients, wherein the double-layer mask consists of a first mask layer and a second mask layer, wherein the first mask layer contains a first matrix and a first essence, and the second mask layer contains a second matrix and a second essence; the first essence and / or the second essence contain a variety of effective ingredients and penetration enhancers with different functions, and have good and diverse penetration effects.

Description

Technical Field

[0001] The present application belongs to the technical field of facial masks in cosmetics. Specifically, the present application provides a highly efficient and highly permeable double-layer facial mask containing small molecule essence ingredients. Background Art

[0002] It usually takes a certain amount of time for the mask liquid to penetrate into the stratum corneum of the facial epidermis to be absorbed and utilized. The skin's absorption of the mask liquid greatly affects the effect of the mask liquid on the facial skin.

[0003] In addition to extending the attachment time, optimizing the shape and matrix of the mask sheet, penetration enhancers are often added in the art to promote the absorption of the active ingredients in the mask liquid. The penetration enhancers that can be used in the mask are mainly azone, borneol, peppermint oil, DCMS, etc. These penetration enhancers may bring unexpected irritation and allergy risks to the mask ingredients. If ingredients with penetration-promoting functions can be found in plant extracts that can be used in cosmetics and masks, the above problems can be solved while providing additional effects for mask products.

[0004] Currently, only a small amount of plant volatile oils and penetration-enhancing short peptides have been discovered as such ingredients. However, when volatile oils are used in facial masks, it will bring difficulties to the preparation and preservation of mask liquids. Summary of the invention

[0005] In response to the above situation, on the one hand, the present application provides a high-efficiency and high-penetration double-layer mask containing small molecule essence ingredients, the double-layer mask consisting of a first mask layer and a second mask layer, the first mask layer comprising a first matrix and a first essence, and the second mask layer comprising a second matrix and a second essence; the first essence and / or the second essence contain a penetration enhancer.

[0006] Furthermore, the first essence is composed of deionized water and one or more effective ingredients selected from niacinamide, tranexamic acid, resveratrol, ascorbyl glucoside, galactose yeast fermentation product, olive leaf extract, purslane extract, bifid yeast fermentation product lysate, hydroxyphenylpropionamide benzoic acid, sodium DNA, adenosine, bosine, collagen, caffeine, aescin, bifid yeast fermentation product filtrate, C coffee repair peptide, ascorbyl tetraisopalmitate, Melaleuca alternifolia leaf extract, white willow bark extract, colloidal sulfur, dipotassium glycyrrhizate, Scutellaria baicalensis extract, matrine, capryloyl glycine, North American witch hazel water, lactobionic acid compound white willow bark extract, and silymarin.

[0007] Furthermore, the second essence is composed of deionized water and one or more effective ingredients selected from the group consisting of rosa damascena flower water, inositol, phospholipids, avocado butter, lactic acid, niacinamide, undecylenoyl phenylalanine, 4-butylresorcinol, magnolia sieboldii extract, ectoine, ceramide NP, fibronectin, Centella asiatica extract, dipotassium glycyrrhizate, panthenol, water-locking magnet, HEPE, hydrolyzed collagen, C-caffeine repair peptide, ergothioneine, lactobacillus fermentation product, β-glucan, witch hazel water, capryloyl glycine, sodium hyaluronate or its derivatives, and biotin VB7.

[0008] Furthermore, the first matrix is ​​a Tencel fiber dressing, and / or the second matrix is ​​a carboxymethyl cellulose fiber dressing or a composite dressing formed by blending carboxymethyl cellulose fiber and chitin fiber.

[0009] Furthermore, the content of the penetration enhancer in the first essence and / or the second essence is 0.5% w / v-1% w / v.

[0010] Furthermore, the penetration enhancer is thyme water extract or Elsholtzia water extract.

[0011] Furthermore, the thyme water extract is prepared according to the following method:

[0012] Take dried thyme, crush it, add it into water with a mass of 10-20 times of the dried thyme, reflux and extract for 2 hours, collect the extract, filter it, and obtain the thyme water extract through rotary evaporation and drying steps.

[0013] Furthermore, the Elsholtzia water extract is prepared according to the following method:

[0014] The dried Elsholtzia ciliata is taken, crushed and added into water with a mass of 10-20 times of the dried Elsholtzia ciliata, refluxed and extracted for 2 hours, the extract is collected and filtered, and the Elsholtzia ciliata water extract is obtained through rotary evaporation and drying steps.

[0015] Furthermore, the penetration enhancer is an enzyme-modified thyme water extract or an Elsholtzia water extract.

[0016] Furthermore, the enzyme-modified thyme extract is prepared according to the following method:

[0017] The thyme water extract is prepared into a 4-6% w / v solution, the pH is adjusted to 7-8, 400-600 U / mL of trypsin is added, and the solution is treated at 45-55° C. for 1.5-3 hours to inactivate the enzyme, and the enzyme-modified thyme water extract is obtained through rotary evaporation and drying steps.

[0018] Furthermore, the enzyme-modified Elsholtzia water extract is prepared according to the following method:

[0019] The water extract of Elsholtzia ciliata is prepared into a 4-6% w / v solution, the pH is adjusted to 7-8, 400-600 U / mL of ficin is added, the solution is treated at 45-55° C. for 1.5-3 hours to inactivate the enzyme, and the enzyme-modified water extract of Elsholtzia ciliata is obtained through rotary evaporation and drying steps.

[0020] On the other hand, the present application provides the use of the above-mentioned thyme water extract or Elsholtzia water extract, or the above-mentioned enzyme-modified thyme water extract or Elsholtzia water extract in the preparation of a cosmetic penetration enhancer.

[0021] Furthermore, the cosmetic penetration enhancer is a penetration enhancer for facial masks.

[0022] According to the existing knowledge in the field, thyme or Elsholtzia extracts can be used in the field of cosmetics, and can provide antioxidant, tyrosine kinase inhibition, water retention and other effects. The present application uses thyme or Elsholtzia extracts as penetration enhancers, which can effectively improve the penetration effect and provide its inherent skin care effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The penetration-enhancing effect of thyme extract modified by various enzymes on matrine;

[0024] Figure 2 The penetration-enhancing effect of various enzyme-modified Elsholtzia extracts on matrine;

[0025] Figure 3 The penetration-enhancing effect of enzyme-modified or unmodified extracts on niacinamide;

[0026] Figure 4 The penetration-enhancing effect of enzyme-modified or unmodified extracts on panthenol;

[0027] Figure 5 The penetration-enhancing effect of enzyme-modified or unmodified extracts on hyaluronic acid.

[0028] Example 1 Basic research methods

[0029] Preparation of extracts:

[0030] In order to ensure stable and controllable quality, thyme and elsholtzia extracts were homemade during the research phase, and commercially available water extracts could be used later, and the extraction method, dosage, and processing steps could be adjusted based on practical results.

[0031] Take dried thyme or elsholtzia (produced in Shandong and Gansu, respectively), crush it and add it to water with a mass of 15 times that of dried thyme or elsholtzia, reflux and extract for 2 hours, collect the extract and filter it; the extract after filtration can be directly used after refrigeration, or obtain thyme or elsholtzia extract through rotary evaporation and drying. Penetration effect test (refer to GB / T27818-2011):

[0032] Ex vivo skin preparation:

[0033] Take healthy male SD rats (220g±10g); use 10% Na2S to remove the abdominal hair and wash; feed overnight and then kill; peel off the abdominal skin and remove fat, mucus and other debris; wash with saline; use it immediately or store it in saline at 4°C.

[0034] Transdermal absorption test:

[0035] The ex vivo skin was fixed with the keratin surface facing upward on the diffusion cell of a transdermal tester (TP-6, Jinyang Wanda); the receiving solution was saline, and the temperature was set at 37°C; the diffusion area was 3.2 cm 2 ; After regular sampling, add an equal amount of receiving solution (the sample is a solution or a mask sheet applied to the surface of the keratin).

[0036] The removed samples were filtered through a 0.22-micron membrane; matrine, niacinamide, panthenol, and sodium hyaluronate were determined by liquid chromatography (referring to the provisions of the 2020 edition of the Pharmacopoeia, using EclipsePlus C18 column or CK08EH column, Shimadzu LC-20A liquid chromatography instrument liquid chromatography and perchloric acid titration method for determination); the cumulative transdermal amount and the corresponding permeability were calculated; the Qt curve for 1 h can be further drawn, and the transdermal rate can be further calculated (4 parallel experiments for each formulation).

[0037] Example 2: Penetration-enhancing effect of thyme or elsholtzia water extract on various facial mask ingredients

[0038] Four representative molecules, matrine, niacinamide, panthenol, and sodium hyaluronate (nominal 1300 kDa-1500 kDa), were selected and prepared into 0.8% w / v aqueous solutions to study the effects of thyme or Elsholtzia water extracts on their penetration. The results are shown in Table 1:

[0039] Table 1 Effects of different penetration enhancers on the penetration of representative molecules

[0040]

[0041]

[0042]

[0043] The results showed that although there were still differences compared with low concentrations of azone, thyme extract and Elsholtzia ciliata extract had clear penetration-enhancing effects on both small and large molecular components.

[0044] Example 3 Enzyme modification of thyme or elsholtzia water extract

[0045] Considering that the representative small and large molecular hyaluronic acids with different structures and functions have a penetration-promoting effect, and the volatile oil components used are not rich in phenols, terpenes, olefins, and flavonoids. Considering that the penetration-promoting components of the water extract may come from its water-soluble protein, the penetration-promoting peptides that have the effect of separating cells or keratin (currently there are few precedents of plant extract polysaccharide components having a penetration-promoting effect). We used a variety of food-grade proteases to modify thyme or Elsholtzia water extracts, and the specific method determined after the initial screening conditions was:

[0046] The thyme or Elsholtzia water extract is prepared into a 5% w / v solution, the pH is adjusted to 7.5, 500 U / mL of different proteases (trypsin, pepsin, papain, ficin, bromelain; food grade, summer-rich) are added, the solution is treated at 50° C. for 2 hours, the enzyme is inactivated at 85-90° C. for 5 minutes, and the enzyme-modified thyme or Elsholtzia extract is obtained through rotary evaporation and drying steps.

[0047] Taking matrine as a representative, the penetration-enhancing effect of the above enzyme-modified extract was tested (as shown in Example 1). The results are as follows Figure 1 shown.

[0048] The results showed that enzymatic hydrolysis with pepsin had an adverse effect on the penetration-enhancing effect of the extracts, while enzymatic modification with trypsin and ficin significantly improved the penetration-enhancing effect of thyme or Elsholtzia water extracts, approaching the level of azone at the same concentration. The permeation rate of matrine was also significantly improved after calculation (due to poor r values ​​of multiple Qt curves, the rates listed in Table 2 are only approximate levels, not accurate values).

[0049] Table 2 Effect of penetration enhancers on the penetration rate of matrine

[0050]

[0051]

[0052] Example 4 Effect of enzyme-modified extracts on the permeation of other representative molecules

[0053] The penetration-enhancing effect of the enzyme-modified extract on niacinamide, panthenol, and hyaluronic acid was further verified. Figure 3-5 As shown. The results show that the penetration-enhancing effect of the thyme water extract modified by trypsin and the Elsholtzia water extract modified by ficin is improved compared with the unmodified extract. This penetration-enhancing effect and its improvement are common in many different types of small molecules and macromolecules. It is predicted that the principle is the production of penetration-enhancing small proteins or peptides that have the effect of dissociating cells or keratin. Further separation of these small proteins or peptides can further verify the effect and expand the application.

[0054] Embodiment 5 facial mask example

[0055] The physical mask product is a high-efficiency and high-permeability double-layer mask, consisting of an independent first mask layer and a second mask layer. The mask base material of the first mask layer is a Tencel fiber dressing, which consists of 4 pieces, namely a forehead mask patch, a nose mask patch and two cheek mask patches; the base material of the second mask layer is a carboxymethyl cellulose fiber dressing or a composite dressing formed by a blend of carboxymethyl cellulose fiber and chitin fiber.

[0056] The preparation method of the double-layer mask is as follows:

[0057] According to the formula, prepare the first essence and the second essence respectively;

[0058] Cut the Tencel fiber non-woven fabric into 4 partial mask patches, and cut the carboxymethyl cellulose fiber non-woven fabric into a whole mask patch;

[0059] Immerse 4 partial mask patches in the first essence liquid, and immerse the entire mask patch in the second essence liquid. After the mask liquid fully absorbs the essence liquid (about 1 hour), take it out.

[0060] Double layer mask 1:

[0061] First Essence: Niacinamide, tranexamic acid, resveratrol, ascorbyl glucoside, galactosyl yeast fermentation product, prepared with deionized water;

[0062] Second essence: Rosa damascena flower water, inositol, phospholipids, avocado butter, lactic acid, niacinamide, undecylenoyl phenylalanine, 4-butylresorcinol, Magnolia sieboldii extract, thyme or Elsholtzia water extract 0.5% w / v-1% w / v, prepared with deionized water.

[0063] Double layer mask 2:

[0064] First essence: olive leaf extract, purslane extract, bifida yeast fermentation product lysate, hydroxyphenylpropionamide benzoic acid, prepared with deionized water;

[0065] The second essence: Ectoin, ceramide NP, fibronectin, Centella asiatica extract, dipotassium glycyrrhizinate, panthenol, thyme or Elsholtzia water extract 0.5% w / v-1% w / v, prepared with deionized water.

[0066] Double layer mask 3:

[0067] First essence: DNA sodium, adenosine, bosacein, collagen, caffeine, aescin, bifida yeast fermentation product filtrate, C coffee repair peptide, ascorbyl tetraisopalmitate, prepared with deionized water;

[0068] Second essence: Ecdoin, ceramide NP, water-locking magnet, HEPE, fibronectin, hydrolyzed collagen, C-coffee repair peptide, ergothioneine, thyme or Elsholtzia water extract 0.5% w / v-1% w / v, prepared with deionized water.

[0069] Double layer mask 4:

[0070] First essence: Melaleuca alternifolia leaf extract, willow bark extract, colloidal sulfur, dipotassium glycyrrhizinate, scutellaria baicalensis extract, matrine, capryloyl glycine, prepared with deionized water;

[0071] The second essence: Centella asiatica extract, lactobacillus fermentation product, ectoine, β-glucan, niacinamide, thyme or Elsholtzia water extract 0.5% w / v-1% w / v, prepared with deionized water.

[0072] Double layer mask 5:

[0073] First essence: double zinc and three bacteria dew, witch hazel water, lactobionic acid compound white willow bark extract, silymarin, prepared with deionized water;

[0074] Second essence: witch hazel water, capryloyl glycine, ectoine, ceramide NP, niacinamide, sodium hyaluronate derivative, biotin VB7, thyme or elsholtzia water extract 0.5% w / v-1% w / v, prepared with deionized water.

Claims

1. A highly efficient and highly permeable double-layer mask containing small molecule essence ingredients, characterized in that: The double-layer mask consists of a first mask layer and a second mask layer, wherein the first mask layer contains a first matrix and a first essence, and the second mask layer contains a second matrix and a second essence; the second essence contains a penetration enhancer; The ingredients of the first essence and the second essence are as follows: The first essence comprises: niacinamide, tranexamic acid, resveratrol, ascorbyl glucoside, and galactosyl yeast fermentation product, and is prepared with deionized water; the second essence comprises: rosa damascena flower water, inositol, phospholipids, avocado tree butter, lactic acid, niacinamide, undecylenyl phenylalanine, 4-butylresorcinol, magnolia sieboldii extract, and a penetration enhancer of 0.5% w / v-1% w / v, and is prepared with deionized water; or, The first essence comprises: olive leaf extract, purslane extract, bifida yeast fermentation product lysate, hydroxyphenylpropionamide benzoic acid, and is prepared with deionized water; the second essence comprises: ectoine, ceramide NP, fibronectin, Centella asiatica extract, dipotassium glycyrrhizinate, panthenol, and a penetration enhancer of 0.5% w / v-1% w / v, and is prepared with deionized water; or, The first essence comprises: Melaleuca alternifolia leaf extract, willow bark extract, colloidal sulfur, dipotassium glycyrrhizinate, scutellaria baicalensis extract, matrine, capryloyl glycine, and is prepared with deionized water; the second essence comprises: Centella asiatica extract, lactobacillus fermentation product, ectoine, β-glucan, niacinamide, and a penetration enhancer of 0.5% w / v-1% w / v, and is prepared with deionized water; The penetration enhancer is an enzyme-modified thyme water extract or an enzyme-modified Elsholtzia water extract; The preparation method of the enzyme-modified thyme extract is as follows: The method comprises the steps of taking dried thyme, crushing the thyme, adding the thyme to water with a mass of 10 to 20 times the mass of the dried thyme, refluxing and extracting the thyme for 2 hours, collecting the extract, filtering the extract, and performing rotary evaporation and drying to obtain a thyme water extract; preparing the thyme water extract into a 4 to 6% w / v solution, adjusting the pH to 7 to 8, adding 400 to 600 U / mL of trypsin, treating the solution at 45 to 55° C. for 1.5 to 3 hours, inactivating the enzyme, and performing rotary evaporation and drying to obtain an enzyme-modified thyme water extract; The preparation method of the enzyme-modified Elsholtzia water extract is as follows: The dried Elsholtzia ciliata is crushed and added into water with a mass of 10-20 times of the dried Elsholtzia ciliata, and refluxed for extraction for 2 hours. The extract is collected and filtered, and a water extract of the Elsholtzia ciliata is obtained through rotary evaporation and drying. The water extract of the Elsholtzia ciliata is prepared into a solution with a weight of 4-6%, the pH value is adjusted to 7-8, 400-600 U / mL of ficin is added, and the solution is treated at 45-55°C for 1.5-3 hours to inactivate the enzyme, and an enzyme-modified water extract of the Elsholtzia ciliata is obtained through rotary evaporation and drying.

2. The double-layer mask according to claim 1, wherein the first matrix is ​​a Tencel fiber dressing, and / or the second matrix is ​​a carboxymethyl cellulose fiber dressing or a composite dressing formed by a blend of carboxymethyl cellulose fiber and chitin fiber.

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