A plant extract composite antibacterial agent and its application

Abstract

The present invention discloses a plant extract composite antibacterial agent and application, the plant extract composite antibacterial agent comprises the following raw materials in percentage by mass: 20-70% of antibacterial agent, the antibacterial agent comprises at least two mixtures of aconite extract, orientalis leaf extract, and ginkgo nut extract; the present invention can inhibit the activity of tyrosinase by giving full play to the synergistic effect between the extracts of the antibacterial agent, thereby reducing the synthesis of melanin and achieving a good whitening effect. The plant extract composite antibacterial agent can be used in cosmetics, and has the advantages of low dosage, safety and effectiveness, and natural low stimulation.

Description

Technical Field

[0001] The invention belongs to the field of plant extracts, and in particular relates to a plant extract composite antibacterial agent and application thereof. Background Art

[0002] In recent years, with the improvement of living standards, people pay more and more attention to the maintenance of their skin, and the use of cosmetics and skin care products has become more frequent. Preservatives are an indispensable ingredient in cosmetics. Adding preservatives can inhibit the growth of microorganisms in cosmetics. Selecting efficient and safe preservatives is one of the keys to the development of cosmetic formulas. However, existing preservatives are difficult to achieve good whitening effects while maintaining good preservative effects. Therefore, the importance of developing a natural extract preservative with good whitening effects is becoming increasingly significant.

[0003] Qinpi is the dried bark of Fraxinus affine, Fraxinus chinensis, Fraxinus apicalensis or Fraxinus sibiricum, which belongs to the Oleaceae family. It has the effects of clearing away heat and dampness, astringing and stopping diarrhea, stopping leukorrhea and improving eyesight. Qinpi is mainly produced in Shaanxi, Hebei, Henan, Shanxi, Liaoning, Jilin and other places. It is peeled in spring and autumn and dried in the sun. Studies have shown that Qinpi mainly contains chemical components such as coumarins, lignans, and cyclopentanes; and modern pharmacological experiments have shown that it has antibacterial, anti-inflammatory, analgesic, anti-tumor, antitussive and expectorant effects and lowering blood uric acid.

[0004] Platycladus orientalis, also known as Platycladus orientalis, is an evergreen tree. Its young branches, leaves and fruits can all be used as medicine. It tastes bitter and astringent, is slightly cold in nature, and enters the lung, liver and large intestine meridians. It has the effects of cooling blood and stopping bleeding, blackening hair, and relieving cough and asthma. It is mainly used for bleeding diseases caused by blood heat, and has the effects of relieving cough, removing phlegm, lowering blood pressure, and preventing hair loss. The kernels in its fruits have the effects of nourishing the heart and calming the mind, and moistening the intestines and relieving constipation.

[0005] Ginkgo nut, commonly known as white nut, is a nutritious high-end tonic. The fruit of the ginkgo tree is the ginkgo nut, which is a kind of traditional Chinese medicine. The functions of ginkgo nut include delaying aging, beautifying the skin, preventing cardiovascular and cerebrovascular diseases, and disinfecting and sterilizing. Summary of the invention

[0006] In view of the above-mentioned deficiencies in the prior art, the present invention can inhibit the activity of tyrosinase by giving full play to the synergistic effect between the extracts of the antibacterial agent, thereby reducing the synthesis of pigments and achieving a better whitening effect. The plant extract composite antibacterial agent can be used in cosmetics, and has the advantages of low dosage, safety and effectiveness, and natural low irritation.

[0007] The object of the present invention is to provide a plant extract composite antibacterial agent, comprising the following raw materials in percentage by weight: 20-70% of the antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of a Fraxinus acuminata extract, a Platycladus orientalis leaf extract and a Ginkgo nut extract.

[0008] In some embodiments of the present invention, the plant extract composite antibacterial agent comprises the following raw materials in percentage by weight: 20-50% of antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of the extract of Fraxinus acuminata, the extract of Platycladus orientalis leaves, and the extract of Ginkgo biloba nuts.

[0009] In some embodiments of the present invention, the plant extract composite antibacterial agent comprises the following raw materials in percentage by weight: 20-40% of antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of the extract of Fraxinus acuminata, the extract of Platycladus orientalis leaves, and the extract of Ginkgo biloba nuts.

[0010] In some embodiments of the present invention, the plant extract composite antibacterial agent includes the following raw materials in percentage by weight: 30% antibacterial agent, the antibacterial agent includes a mixture of at least two of the extract of Fraxinus acuminata, the extract of Platycladus orientalis leaves, and the extract of Ginkgo biloba nuts.

[0011] In some embodiments of the present invention, the plant extract composite antibacterial agent further comprises 30-80% of a solvent.

[0012] In some embodiments of the present invention, the plant extract composite antibacterial agent further comprises 50-80% of a solvent.

[0013] In some embodiments of the present invention, the plant extract composite antibacterial agent further comprises 60-80% of a solvent.

[0014] In some embodiments of the present invention, the plant extract composite antibacterial agent further comprises 70% solvent.

[0015] In some embodiments of the present invention, the solvent includes at least one of 1,3-butanediol, 1,3-propylene glycol, 1,2-propylene glycol, 1,2-butanediol, 1,4-butanediol, 1,2-pentanediol, 1,5-pentanediol, isopentyl glycol, isohexanediol, 1,2-hexanediol, dipropylene glycol, water, glycerol, and methylpropanediol.

[0016] In some embodiments of the present invention, the mass ratio of the Fraxinus acuminata extract to the Platycladus orientalis leaf extract is 2-6:1-4.

[0017] In some embodiments of the present invention, the mass ratio of the Fragrant Cortex Acuminatum extract to the Platycladus orientalis leaf extract is 2:1.

[0018] In some embodiments of the present invention, the mass ratio of the Fraxinus acuminata extract to the Ginkgo biloba nut extract is 2-5:1-4.

[0019] In some embodiments of the present invention, the mass ratio of the Fraxinus acuminata extract to the Ginkgo biloba nut extract is 2:1.

[0020] In some embodiments of the present invention, the mass ratio of the Platycladus orientalis leaf extract to the Ginkgo biloba nut extract is 1-4:1-4.

[0021] In some embodiments of the present invention, the mass ratio of the Platycladus orientalis leaf extract to the Ginkgo biloba nut extract is 1:1.

[0022] In some embodiments of the present invention, the mass ratio of the Fraxinus acuminata extract, Platycladus orientalis leaf extract and Ginkgo biloba nut extract is 2-6:1-4:1-4.

[0023] In some embodiments of the present invention, the mass ratio of the Fraxinus acuminata cortex extract, Platycladus orientalis leaf extract and Ginkgo biloba nut extract is 15:7:8.

[0024] In some embodiments of the present invention, the extraction process of the extract of the Fraxinus acuminata comprises the following steps:

[0025] The cortex quinquefolium is crushed, the cortex quinquefolium powder is extracted with a first solvent, ultrasonically extracted, and the extract is purified to obtain the cortex quinquefolium extract.

[0026] In some embodiments of the present invention, the first solvent comprises an ethanol aqueous solution with a mass concentration of 40-80%.

[0027] In some embodiments of the present invention, the mass ratio of the Fraxinus acuminata powder to the first solvent is 1:6-10.

[0028] In some embodiments of the present invention, after the Fraxinus acuminata is crushed, it is also sieved through a 60-100 mesh sieve.

[0029] In some embodiments of the present invention, the extraction temperature is 40-70° C. and the extraction time is 1-2 hours.

[0030] In some embodiments of the present invention, the ultrasonic extraction time is 1-3 hours.

[0031] In some embodiments of the present invention, the extraction process of the Platycladus orientalis leaf extract comprises the following steps:

[0032] The Platycladus orientalis leaves are crushed, the Platycladus orientalis leaf powder is subjected to ultrasonic extraction with a second solvent, and the extract is purified to obtain the Platycladus orientalis leaf extract.

[0033] In some embodiments of the present invention, the second solvent comprises an ethanol aqueous solution with a mass concentration of 60-80%.

[0034] In some embodiments of the present invention, the mass ratio of the Platycladus orientalis leaf powder to the second solvent is 1:6-10.

[0035] In some embodiments of the present invention, after the Platycladus orientalis leaves are crushed, they are also sieved through a 60-100 mesh sieve.

[0036] In some embodiments of the present invention, the temperature of the ultrasonic extraction is 50-70° C. and the time is 2-6 hours.

[0037] In some embodiments of the present invention, the extraction process of the ginkgo nut extract comprises the following steps:

[0038] The ginkgo nuts are crushed, the ginkgo nut powder is extracted with a third solvent, and the extract is concentrated and purified to obtain the ginkgo nut extract.

[0039] In some embodiments of the present invention, the third solvent comprises an ethanol aqueous solution with a mass concentration of 50-80%.

[0040] In some embodiments of the present invention, the mass ratio of the ginkgo nut powder to the third solvent is 1:8-10.

[0041] In some embodiments of the present invention, after the ginkgo nuts are crushed, they are also sieved through a 60-100 mesh sieve.

[0042] In some embodiments of the present invention, the extraction temperature is 40-60° C. and the extraction time is 2-6 hours.

[0043] Another object of the present invention is to provide an application of the plant extract composite antibacterial agent in the preparation of cosmetics, wherein the cosmetics contain the plant extract composite antibacterial agent, and the cosmetics include at least one of facial mask liquid, emulsion, cream, toner, essence, stock solution, facial cleanser, lotion, perfume, makeup remover, liquid foundation, foundation cream, concealer cream, rouge, lipstick, eye shadow, and blush.

[0044] In some embodiments of the present invention, the amount of the plant extract composite antibacterial agent is 0.5-5% of the cosmetics in terms of mass percentage.

[0045] Compared with the prior art, the present invention has the following beneficial effects:

[0046] (1) The components of the plant extract composite antibacterial agent provided by the present invention are all common plant extracts, which can replace traditional preservatives and have the advantages of being safe, mild and non-irritating, and will not cause skin allergic reactions.

[0047] (2) The present invention can inhibit the activity of tyrosinase by giving full play to the synergistic effect between the extracts of the antibacterial agent, thereby reducing the synthesis of pigments and achieving a good whitening effect. The plant extract composite antibacterial agent can be used in cosmetics and has the advantages of low dosage, safety and effectiveness, and natural low irritation. DETAILED DESCRIPTION

[0048] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0049] Example 1

[0050] This embodiment provides an extraction process for the extract of the Fraxinus acuminata, and the specific extraction process is as follows:

[0051] Take 55kg of pointed leaf qinpi, crush it with a traditional Chinese medicine grinder, pass through an 80-mesh sieve to obtain pointed leaf qinpi powder. Take 50kg of the sieved pointed leaf qinpi powder, add 6-10 times the amount of 40-80% ethanol aqueous solution, mix well and then leaching, generally leaching for 1-2 hours, the temperature is kept at about 55°C, after leaching, transfer the extraction mixture to an ultrasonic extraction device, use a high-power dual-frequency ultrasonic device, ultrasonic extraction for 2h, after the extraction is completed, filter and remove impurities from the extract, and remove water and ethanol at an environment not higher than 60°C, the remaining pointed leaf qinpi extract is further ultrafiltered and refined to obtain the pointed leaf qinpi extract.

[0052] Example 2

[0053] This embodiment provides an extraction process of Platycladus orientalis leaf extract, and the specific extraction process is as follows:

[0054] Take 55kg of Platycladus orientalis leaves, crush them with a Chinese medicine grinder, and then pass them through an 80-mesh sieve for later use. Take 50kg of the sieved Platycladus orientalis leaf fine powder and add it to an extraction container, add 6-10 times the amount of 60-80% ethanol aqueous solution, and perform ultrasonic extraction 2-3 times at a temperature of 50-70°C and normal pressure, each time for 1-2 hours, combine the extracts, filter the extracts to remove impurities, and remove water and ethanol at an environment not higher than 60°C, and further ultrafilter the remaining Platycladus orientalis leaf extract to remove impurities and purify, so as to obtain Platycladus orientalis leaf extract.

[0055] Example 3

[0056] This embodiment provides an extraction process for ginkgo nut extract, and the specific extraction process is as follows:

[0057] Take 55kg of ginkgo nuts, crush them with a Chinese medicine grinder, and pass through an 80-mesh sieve to obtain ginkgo nut powder. Take 50kg of the sieved ginkgo nut powder, add 8-10 times the amount of 50-80% ethanol aqueous solution, mix evenly, heat and reflux to extract 2-3 times, each time for 1-2 hours, and the extraction temperature is about 50°C. Combine the extracts, reduce pressure and vacuum concentrate at 65°C to obtain a ginkgo nut extract concentrate, disperse it with an appropriate amount of pure water, extract it with ethyl acetate, and concentrate the ethyl acetate layer to obtain an extract; the extract is adsorbed by a macroporous resin, eluted with 95% ethanol by volume, and the eluent is concentrated and dried to obtain a ginkgo nut extract.

[0058] Preparation of plant extract compound antibacterial agent:

[0059] Table 1. Raw material composition of plant extract composite antibacterial agent.

[0060] formula Raw materials and mass percentage Example 4 20% Fraxinus acuminata extract + 10% Platycladus orientalis leaf extract + 70% 1,3-butylene glycol Example 5 20% Fraxinus acuminata extract + 10% Ginkgo biloba nut extract + 70% 1,3-butylene glycol Example 6 15% Oriental Platycladus orientalis leaf extract + 15% Ginkgo biloba nut extract 70% 1,3-butylene glycol Example 7 15% Fraxinus acuminata extract + 7% Platycladus orientalis leaf extract + 8% Ginkgo biloba nut extract + 70% 1,3-butylene glycol Comparative Example 1 30% Fraxinus acuminata extract + 70% 1,3-butylene glycol Comparative Example 2 30% Oriental Platycladus orientalis leaf extract + 70% 1,3-Butanediol Comparative Example 3 30% Ginkgo Nut Extract + 70% 1,3-Butanediol

[0061] According to the raw materials and mass percentages in Table 1, the above extracts and 1,3-butanediol were added to a 250 mL flask equipped with a thermometer and a stirrer, the temperature was raised to 40°C, and stirred for 30 to 50 minutes until completely mixed. After cooling to room temperature, insoluble impurities were removed by filtration to obtain a plant extract composite antibacterial agent.

[0062] Antiseptic efficacy test:

[0063] Test strains: The bacteria used in this experiment were a mixed strain of Pseudomonas aeruginosa ATCC9027, Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538; the fungi were Candida albicans ATCC10231 and Aspergillus niger ATCC16404, all of which were from Guangdong Provincial Microbiological Culture Collection Center.

[0064] The initial inoculum volume of the mixed bacteria used in the test was 10 5 ~10 6 CFU / g, the initial inoculum of Candida albicans and Aspergillus niger was 10 4 ~10 5 CFU / g, the bacterial content was determined on the 7th, 14th and 28th days, and the logarithmic value of the reduction in the number of various microorganisms at the corresponding time nodes was calculated. The preservatives in Tables 2 to 4 were respectively the plant extract composite antibacterial agents of Examples 4 to 7 and Comparative Examples 1 to 3 in Table 1; the test systems were mask liquid, moisturizing lotion and repair cream. The formula and production process are shown in Tables 2 to 4.

[0065] Table 2. Facial mask liquid ingredients and production process.

[0066]

[0067] Table 3. Ingredients and production process of moisturizing lotion.

[0068]

[0069]

[0070] Table 4. Repair cream ingredients and production process.

[0071]

[0072]

[0073] The test results were judged as follows: the number of bacteria decreased by 3 orders of magnitude on the 7th day, and decreased by 3 orders of magnitude on the 14th and 28th days without increasing; the number of Candida albicans decreased by 1 order of magnitude on the 7th day, and decreased by one order of magnitude on the 14th and 28th days without increasing; the number of Aspergillus niger did not increase on the 14th day, and decreased by one order of magnitude on the 28th day, which was considered passed. The results are shown in Tables 5 to 7.

[0074] Table 5. Test data of antiseptic efficacy of facial mask liquid.

[0075]

[0076] Table 6. Test data of the preservative efficacy of moisturizing lotions.

[0077]

[0078]

[0079] Table 7. Test data of the antiseptic efficacy of the repair cream.

[0080]

[0081] As shown in Tables 5-7, the plant extract composite antibacterial agent prepared in Examples 4 to 7 passed the antiseptic efficacy test in the three test systems of mask liquid, moisturizing lotion, and repair cream, and has a strong antiseptic effect. The antibacterial spectrum and antibacterial ability of the three test systems of mask liquid, moisturizing lotion, and repair cream prepared by the single-component antibacterial agent in Comparative Examples 1 to 3 have defects in antiseptic efficacy and failed the antiseptic efficacy test. It can be seen that the plant extract composite antibacterial agent has a synergistic antiseptic effect.

[0082] Test of tyrosinase activity inhibition rate:

[0083] The synthesis of melanin is affected by tyrosinase, which can catalyze the hydroxylation of L-tyrosine into dopa and oxidize dopa into dopaquinone, which forms the final reaction product, melanin. Test substances with tyrosinase activity inhibition can slow down the tyrosinase-catalyzed conversion of L-tyrosine into dopaquinone. By measuring the absorbance of dopaquinone at 475nm, raw materials with tyrosinase activity inhibition can reduce the conversion of dopa into dopaquinone, thereby reducing the absorbance value. The inhibitory effect of the test substance on tyrosinase activity is evaluated based on the change in absorbance. Refer to T / GDCA 006-2021 Test method for inhibition of tyrosinase activity by cosmetic raw materials (in vitro method).

[0084] The specific method is as follows:

[0085] Solution preparation:

[0086] 1. Preparation of tyrosinase solution: Prepare tyrosinase solution to 500 U / ml with PBS buffer, divide into aliquots, and store at -20℃ to avoid secondary freezing and thawing.

[0087] 2. Preparation of L-tyrosine solution: Take 0.025g L-tyrosine and dissolve it in an appropriate amount of PBS buffer solution, use ultrasonic dissolution to make it fully dissolved, and then use PBS to make the volume to 50mL to prepare a 0.5g / L L-tyrosine solution. The solution should be prepared and used immediately.

[0088] 3. Preparation of positive control solution: The positive control kojic acid was diluted with ultrapure water to a series of mass concentration gradient solutions of 0.040 g / L, 0.020 g / L, 0.010 g / L, 0.008 g / L, 0.005 g / L, 0.002 g / L, and 0.001 g / L.

[0089] 4. Sample solution preparation: Use PBS buffer to prepare the sample to a 1% test concentration.

[0090] 6.2 Test Grouping:

[0091] The experiment was divided into solvent background group (T a ), solvent reaction well group (T b ), sample background group (T c ), sample reaction group (T d ). Solvent background group (T a ) are solvent, PBS buffer, and tyrosinase solution; solvent reaction well group (T b ) are L-tyrosine solution, solvent, PBS buffer, and tyrosinase solution; sample background group (T c ) are sample solution, PBS buffer, and tyrosinase solution; sample reaction group (T d ) are L-tyrosine solution, sample solution, PBS buffer, and tyrosinase solution. Each group has three replicate wells.

[0092] Test steps:

[0093] According to the reagent addition amount in Table 8, L-tyrosine solution, sample solution / solvent, and PBS buffer were added to each well in turn, and the well was placed in a full-wavelength microplate reader at 37°C for constant temperature oscillation for 3 minutes, and then incubated for 7 minutes. That is, after adding the sample, it was placed at 37°C for a total of 10 minutes. At the end of the incubation, 20 μL of tyrosinase solution was added to each well in turn, and the well was mixed and reacted at 37°C for 5 minutes ± 5 seconds before reading (it can be placed in a full-wavelength microplate reader at 37°C for constant temperature oscillation for 3 minutes, and then incubated for 2 minutes before reading), and the measurement was performed at a wavelength of 475nm.

[0094] Table 8. Tyrosinase activity inhibition ability loading table

[0095] name <![CDATA[T a (Solvent background hole)]]> <![CDATA[T b (Solvent Reaction Well)]]> <![CDATA[T c (Sample background hole)]]> <![CDATA[T d (Sample Reaction Well)]]> L-Tyrosine solution (μL) 0 40 0 40 Sample solution (μL) 0 0 40 40 Solvent (μL) 40 40 0 0 PBS buffer (μL) 70 30 70 30 Tyrosinase solution (μL) 20 20 20 20 Total (μL) 130 130 130 130

[0096] Data processing:

[0097] Calculation of tyrosinase activity inhibition rate:

[0098]

[0099] In formula (1):

[0100] A a ——average absorbance of solvent background wells;

[0101] A b ——Absorbance of solvent reaction well;

[0102] A c ——Average absorbance of background wells of samples;

[0103] A d ——Absorbance of sample reaction well.

[0104] The positive control half inhibitory concentration (IC 50 value):

[0105] With the positive control concentration as the horizontal axis and the corresponding tyrosinase activity inhibition rate as the vertical axis, a curve was drawn and a linear regression equation was fitted (R 2 ≥0.900) and the positive control concentration corresponding to the 50% inhibition rate of tyrosinase activity was calculated according to the equation. The IC of positive control kojic acid 50 The test is considered valid if it is between 0.005g / L and 0.01g / L.

[0106] Table 9. Tyrosinase activity inhibition test results

[0107]

[0108] From the results in Table 9, it can be seen that the combination of any two or more of the extract from the Chinese frankincense bark, the Chinese arborvitae leaf extract, and the ginkgo nut extract can synergistically inhibit tyrosinase activity, and the inhibitory effect of any two or more of the extract from the Chinese frankincense bark, the Chinese arborvitae leaf extract, and the ginkgo nut extract on tyrosinase activity is better than using any of the above components alone, among which the combination formed by the three extracts has the best inhibitory effect on tyrosinase activity, indicating that there is a synergistic inhibitory effect of tyrosinase activity between two or three components of the extract from the Chinese frankincense bark, the Chinese arborvitae leaf extract, and the ginkgo nut extract.

[0109] Human skin patch test:

[0110] The irritation and safety of the emulsions of Examples 12 to 15 containing the plant extract multi-effect compositions prepared in Examples 4 to 7 were tested. The formula and production process of the emulsions are shown in Table 3.

[0111] Test method: Refer to Chapter 7, Section 2, 6.1 Skin occluded patch test of the 2015 edition of the "Technical Specifications for Safety of Cosmetics". Weigh 0.02 mL of different emulsion products into the patch tester, and set up a blank control group at the same time. The control is the diluent of the cosmetic. 200 healthy volunteers were selected and divided into 4 groups, with 50 in each group, aged 18 to 24 years old, with no history of allergies. After cleaning the inner side of the subject's forearm, take 0.02 mL of sample and add it to the filter paper attached to the patch tester. Apply the patch tester to the inner side of the subject's forearm with non-irritating tape. After pasting, press it lightly with your fingers to make it evenly attached to the skin for 24 hours. Instruct the subject to keep the patch area dry within 24 hours, avoid intense exercise, scratching the patch test area, and long-term sunlight exposure. After 24 hours, remove the tester and mark it. Observe the skin reaction according to the standards in Table 10 at 30 minutes, 24 hours and 48 hours after removing the test patch tester, and record the observation results.

[0112] Table 10. Skin reaction grading standards for occlusive patch test

[0113]

[0114] Table 11. Skin patch test results

[0115]

[0116]

[0117] As can be seen from Table 11, the emulsions of Examples 12 to 15 containing the plant extract composite antibacterial agent of Examples 4 to 7 had no serious adverse reactions, and the subjects basically had negative reactions. The results show that the emulsions of Examples 12 to 15 containing the plant extract composite antibacterial agent prepared by Examples 4 to 7 have low irritation and high safety.

[0118] The mass percentage of the antibacterial agent in the plant extract composite antibacterial agent of the present invention is within the range of 20-70%, and the mass percentage of the solvent is within the range of 30-80% (such as the mass percentage of the antibacterial agent is 20%, 25%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, and the mass percentage of the solvent is 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 75%, 80%, etc.), and / or other solvents are used to extract the pointed leaf qinpi, cypress leaves, and ginkgo nuts (such as using ethanol, methanol, petroleum ether, ethyl acetate and other solvents to extract the pointed leaf qinpi, cypress leaves, and ginkgo nuts), and / or the plant extract composite antibacterial agent uses other solvents (such as 1,3-propylene glycol, 1,2-propylene glycol, 1,2-butylene glycol, etc.). The invention discloses a plant extract composite antibacterial agent comprising: a kind of plant extract composite antibacterial agent, ...

[0119] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that after reading the specification of this application, the technicians can still modify or replace the specific implementation mode of the present invention with equivalents, but these modifications or changes do not deviate from the scope of protection of the pending claims of the present application.

Claims

1. A plant extract composite antibacterial agent, characterized in that: The raw materials include the following mass percentages: 20-70% of an antibacterial agent, wherein the antibacterial agent includes a mixture of at least two of a Fraxinus acuminata extract, a Platycladus orientalis leaf extract, and a Ginkgo nut extract; The mass ratio of the extract of the cortex fraxinii and the extract of the leaves of Platycladus orientalis is 2-6:1-4; The mass ratio of the extract of Fraxinus acuminata to the extract of Ginkgo biloba nuts is 2-5:1-4; The mass ratio of the Platycladus orientalis leaf extract to the Ginkgo biloba nut extract is 1-4:1-4; The extraction process of the Fraxinus acuminata extract comprises the following steps: The quinquefolia cortex is crushed, the quinquefolia cortex powder is leached with a first solvent, ultrasonically extracted, and the extract is purified to obtain a quinquefolia cortex extract; the first solvent is selected from an ethanol aqueous solution with a mass concentration of 40-80%; The extraction process of the Platycladus orientalis leaf extract comprises the following steps: The Platycladus orientalis leaves are crushed, the Platycladus orientalis leaf powder is ultrasonically extracted with a second solvent, and the extract is purified to obtain a Platycladus orientalis leaf extract; the second solvent is selected from an ethanol aqueous solution with a mass concentration of 60-80%; The extraction process of the ginkgo nut extract comprises the following steps: The ginkgo nuts are crushed, the ginkgo nut powder is extracted with a third solvent, the extract is concentrated, dispersed with an appropriate amount of pure water, extracted with ethyl acetate, and the ethyl acetate layer is concentrated to obtain an extract; the extract is adsorbed by a macroporous resin, eluted with 95% ethanol by volume, the eluate is concentrated and dried to obtain a ginkgo nut extract; the third solvent is selected from an ethanol aqueous solution with a mass concentration of 50-80%.

2. The plant extract composite antibacterial agent according to claim 1, characterized in that Also includes 30-80% solvent.

3. The plant extract composite antibacterial agent according to claim 2, characterized in that: The solvent includes at least one of 1,3-butanediol, 1,3-propylene glycol, 1,2-propylene glycol, 1,2-butanediol, 1,4-butanediol, 1,2-pentanediol, 1,5-pentanediol, isoprene glycol, isohexanediol, 1,2-hexanediol, dipropylene glycol, water, glycerol, and methylpropylene glycol.

4. The plant extract composite antibacterial agent according to claim 1, characterized in that The mass ratio of the Fraxinus acuminata bark extract, Platycladus orientalis leaf extract and Ginkgo biloba nut extract is 2-6:1-4:1-4.

5. The plant extract composite antibacterial agent according to claim 1, characterized in that: The mass ratio of the acuminata cortex powder to the first solvent is 1:6-10; The mass ratio of the Platycladus orientalis leaf powder to the second solvent is 1:6-10; The mass ratio of the ginkgo nut powder to the third solvent is 1:8-10.

6. Use of the plant extract composite antibacterial agent according to any one of claims 1 to 5 in the preparation of cosmetics, characterized in that: The cosmetics contain the plant extract composite antibacterial agent, and the cosmetics include at least one of lotion, cream, stock solution, facial cleanser, lotion, perfume, rouge, lipstick, eye shadow and blush.

7. Use of the plant extract composite antibacterial agent according to any one of claims 1 to 5 in the preparation of cosmetics, characterized in that: The cosmetics contain the plant extract composite antibacterial agent, and the cosmetics include at least one of a facial mask liquid, an essence liquid, a toner, a makeup remover, a foundation liquid, a foundation cream, and a concealer cream.

8. The use according to claim 6, characterized in that Calculated by mass percentage, the dosage of the plant extract composite antibacterial agent is 0.5-5% of the cosmetics.

9. The use according to claim 7, characterized in that Calculated by mass percentage, the dosage of the plant extract composite antibacterial agent is 0.5-5% of the cosmetics.

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