A stem cell culture device

Abstract

The present invention discloses a stem cell culture device, which belongs to the field of stem cell culture. A stem cell culture device includes a base and a sealed chamber fixed on the top thereof, and also includes: a bearing platform rotatably connected in the sealed chamber, a shaking part driving the bearing platform to rotate is arranged in the base, wherein a culture dish is placed on the bearing platform, and clamping parts for fixing the culture dish are arranged on both sides of the bearing platform; the present invention drives the bearing platform to rotate periodically, so that the culture dish fixed on the bearing platform undergoes a rotational shaking effect, so that the stem cells in the culture dish are evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of stem cells and improving the culture effect; and the heat generated when the bearing platform is driven to rotate is transported to the heating tank, and the heating of the culture dish is adaptively realized, providing a suitable temperature environment, improving the culture effect of stem cells, and utilizing the heat generated during the working process, which is more energy-saving and environmentally friendly.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, and in particular to a stem cell culture device. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into multiple functional cells. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells. Stem cells are a type of undifferentiated, immature cells that have the potential to regenerate various tissues, organs and the human body. They are called "universal cells" in the medical field. Therefore, the cultivation of stem cells is particularly important.

[0003] At present, most of the existing stem cell culture devices are static culture, so that the stem cells in the culture dish cannot be evenly exposed to nutrients and oxygen, resulting in slow growth and reproduction of stem cells, reducing the culture efficiency of stem cells, and when culturing stem cells, a suitable culture environment needs to be provided, and the existing devices cannot adjust the culture environment in time, which reduces the culture effect of stem cells. Therefore, a stem cell culture device is proposed. Summary of the invention

[0004] The purpose of the present invention is to solve the problems in the prior art that stem cells in the culture dish cannot be evenly exposed to nutrients and oxygen, which reduces the culture efficiency of stem cells; and the culture environment cannot be adjusted in time, which reduces the effect of stem cell culture, and a stem cell culture device is proposed.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A stem cell culture device comprises a base and a sealed chamber fixed on the top thereof, and further comprises: a supporting platform rotatably connected to the sealed chamber, a shaking part for driving the supporting platform to rotate is arranged in the base, a culture dish is placed on the supporting platform, and clamping parts for fixing the culture dish are arranged on both sides of the supporting platform; a planetary gear disc is rotatably connected to the top of the inner cavity of the sealed chamber, a humidifying part for regulating the humidity in the sealed chamber is arranged at the bottom of the planetary gear disc, and the planetary gear disc is driven to rotate by the shaking part.

[0007] In order to improve the culture effect, preferably, the shaking part includes two driving shafts, driving grooves are opened on both sides of the top of the base, the bottom ends of the driving shafts rotate with the bottom of the inner cavity of the base, the top ends of the two driving shafts extend to the center of the inner cavity of the driving grooves on both sides respectively and are fixedly connected with driving gears, the outer wall of the driving shaft is rotatably connected with a positioning slip ring, the positioning slip ring is fixedly connected to the side wall of the inner cavity of the base, the top of the positioning slip ring is slidably sleeved with a driven slip ring, the top of the driven slip ring is fixedly connected with a driven shaft, the driven shaft passes through the driving groove and is fixedly connected to the bottom of the support platform, and the side wall of the driven shaft is fixedly connected with a driven gear, and the driven gear is meshed with the driving gear.

[0008] Preferably, a first motor is fixedly connected to the bottom of the inner cavity of the base, and the output shaft of the first motor is transmission-connected to the driving shafts on both sides via a pulley set, and a linkage shaft is fixedly connected to the output end of the first motor, and the top end of the linkage shaft extends through the planetary gear disk and is fixedly connected to a linkage gear, and the linkage gear is meshingly connected to the planetary gear disk.

[0009] In order to facilitate the provision of a suitable culture environment, preferably, a heating groove is provided in the support platform, the top of the driven slip ring slides in contact with the top of the inner cavity of the base, a heat conduction groove is provided on the side wall of the heating groove, the other end of the heat conduction groove is connected to the inner cavity of the driving groove, sealing rings are fixedly connected on both sides of the base, the inner diameter of the sealing ring is larger than the driving groove and coincides with its central axis, and the top of the sealing ring slides in contact with the bottom of the support platform, a temperature sensor is provided in the heating groove, an electromagnetic valve is provided in the heat conduction groove, and the temperature sensor and the electromagnetic valve are electrically connected.

[0010] In order to improve stability, preferably, the clamping part includes two clamping plates, the two clamping plates are arranged on both sides of the supporting platform and slide along the long axis direction of the clamping plates, the top of the clamping plate is arranged with an arc chamfer and its side wall is provided with a clamping arc groove, and both sides of the supporting platform are provided with a clamping slide groove, the bottom of the clamping plate is slidably connected to the inner wall of the clamping slide groove, and a guide slide rod is fixedly connected in the clamping slide groove, the guide slide rod passes through the side wall of the clamping plate, and the outer wall of the guide slide rod is sleeved with a clamping spring.

[0011] To facilitate humidity control, preferably, the humidifying part includes a humidifying box, which is fixedly connected to the bottom of the planetary gear disk, and a rotating shaft is rotatably connected to the bottom of the humidifying box. A second motor is fixedly connected to the top of the inner cavity of the humidifying box, and an arc-shaped baffle is fixedly connected to the inside of the humidifying box. The top of the rotating shaft passes through the arc-shaped baffle and is fixedly connected to the output end of the second motor. A lifting slider is slidably sleeved on the rotating shaft, and a cutting blade is fixedly connected to the outer wall of the lifting slider, and a spray hole is opened on the side wall of the humidifying box, and the spray hole is connected to the bottom cavity of the humidifying box.

[0012] Furthermore, a limiting groove is provided on the side wall of the rotating shaft, and the protrusion on the inner wall of the lifting slider is inserted into the limiting groove and slidably connected thereto, a plurality of groups of spring telescopic rods are fixedly connected between the lifting slider and the arc baffle, a piston sleeve is fixedly connected to the upper part of the side wall of the rotating shaft, a limiting ring is fixedly connected inside the piston sleeve, a piston slider is slidably connected inside the piston sleeve, a lifting spring is fixedly connected between the piston slider and the limiting ring, a through-groove is provided at the bottom of the piston sleeve, a pull rope is fixedly connected to the top of the lifting slider, the pull rope passes through the through-groove, the limiting ring and the middle part of the lifting spring in sequence and is fixedly connected to the side wall of the piston slider, a magnetic block is fixedly connected to the inner wall of the humidifying box, and there is magnetic repulsion between the magnetic block and the piston slider.

[0013] Furthermore, a humidity sensor is fixedly connected to the bottom of the humidifying box, and the humidity sensor is electrically connected to the second motor.

[0014] In order to improve the sealing effect, preferably, a sealing groove is opened between the sealing chamber and the base, a sealing plate is slidably connected in the sealing groove, a locking sleeve is rotatably connected to the top of the sealing chamber, a locking slider is slidably connected inside the locking sleeve, and a locking spring is fixedly connected between the locking slider and the inner wall of the locking sleeve.

[0015] In order to reduce culture interference, preferably, exhaust pipes are fixedly connected to both sides of the top of the sealed chamber side wall, and a gas pump is fixedly connected to the sealed chamber side wall, the output end of the gas pump is connected to the inner cavity of the sealed chamber, and the input end of the gas pump is connected to an external carbon dioxide gas source, and solenoid valves are provided in the exhaust pipe and the input end of the gas pump.

[0016] Compared with the prior art, the present invention provides a stem cell culture device having the following

[0017] Beneficial effects:

[0018] 1. The stem cell culture device drives the carrier platform to rotate periodically, so that the culture dish fixed on the carrier platform rotates and shakes, so that the stem cells in the culture dish are evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of stem cells and improving the culture efficiency; and the heat generated when the carrier platform is driven to rotate is transmitted to the heating tank, and the culture dish is heated adaptively, providing a suitable temperature environment, improving the culture effect of stem cells, and utilizing the heat generated during the working process, which is more energy-saving and environmentally friendly.

[0019] 2. The stem cell culture device, through the shear flow blades continuously going out and entering the clean water, will slap and cut the clean water to form small mist-like droplets, and after the shear flow blades go out of the clean water surface, an upward thrust will be generated, so that the above-produced mist-like droplets are discharged from the spray hole, and accompanied by the overall rotation effect of the humidification box, the sealed chamber is fully atomized and humidified, thereby adaptively providing a suitable humidity environment and improving the culture effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the overall structure of a stem cell culture device proposed by the present invention;

[0021] Figure 2 A schematic diagram of a partial cross-sectional structure of a stem cell culture device proposed by the present invention when viewed from above;

[0022] Figure 3 A stem cell culture device proposed by the present invention Figure 2 A schematic diagram of the enlarged structure of the middle A area;

[0023] Figure 4 A stem cell culture device proposed by the present invention Figure 2 Schematic diagram of the enlarged structure of the middle B area;

[0024] Figure 5 A schematic diagram of a side view and half section structure of a stem cell culture device proposed by the present invention;

[0025] Figure 6 A stem cell culture device proposed by the present invention Figure 5 Schematic diagram of the enlarged structure of the middle C area;

[0026] Figure 7 A schematic diagram of the internal structure of a humidification box of a stem cell culture device proposed by the present invention;

[0027] Figure 8 A schematic diagram of a half-section structure of a humidification box of a stem cell culture device proposed by the present invention;

[0028] Fig. 9 This is a schematic diagram of the internal structure of a piston sleeve of a stem cell culture device proposed in the present invention.

[0029] In the figure: 1, base; 2, sealing chamber; 21, exhaust pipe; 22, gas pump; 3, bearing platform; 31, heating groove; 32, heat conduction groove; 33, sealing ring; 4, culture dish; 5, planetary gear; 6, driving shaft; 61, driving groove; 62, driving gear; 63, positioning slip ring; 64, driven slip ring; 65, driven shaft; 66, driven gear; 67, first motor; 671, linkage shaft; 672, linkage gear; 68, pulley group; 7, clamping plate; 71, clamping arc groove; 72, clamping slide groove; 73 , guide slide bar; 74, clamping spring; 8, humidifying box; 81, rotating shaft; 811, limiting slide groove; 82, second motor; 83, arc baffle; 84, lifting slide block; 841, cutting flow blade; 85, spray hole; 86, spring telescopic rod; 87, piston sleeve; 871, limiting ring; 872, piston slide block; 873, lifting spring; 874, threading groove; 88, pull rope; 89, magnetic block; 9, sealing groove; 91, sealing plate; 92, locking sleeve; 93, locking slide block; 94, locking spring. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0031] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0032] Example:

[0033] Reference Figure 1-Figure 9 A stem cell culture device comprises a base 1 and a sealed chamber 2 fixed on the top thereof, and further comprises: a bearing platform 3 rotatably connected to the sealed chamber 2, a shaking part for driving the bearing platform 3 to rotate is arranged in the base 1, wherein a culture dish 4 is placed on the bearing platform 3, and clamping parts for fixing the culture dish 4 are arranged on both sides of the bearing platform 3; wherein a planetary gear disc 5 is rotatably connected to the top of the inner cavity of the sealed chamber 2, a humidifying part for adjusting the humidity in the sealed chamber 2 is arranged at the bottom of the planetary gear disc 5, and the planetary gear disc 5 is driven to rotate by the shaking part.

[0034] Reference Figure 2 , Figure 4 , Figure 5 and Figure 6, wherein the shaking part includes two driving shafts 6, driving grooves 61 are provided on both sides of the top of the base 1, the bottom end of the driving shaft 6 rotates with the bottom of the inner cavity of the base 1, the top ends of the two driving shafts 6 extend to the center of the inner cavity of the driving grooves 61 on both sides respectively and are fixedly connected with driving gears 62, the outer wall of the driving shaft 6 is rotatably connected with a positioning slip ring 63, the positioning slip ring 63 is fixedly connected with the side wall of the inner cavity of the base 1, the top of the positioning slip ring 63 is slidably sleeved with a driven slip ring 64, the top of the driven slip ring 64 is fixedly connected with a driven shaft 65, the driven shaft 65 passes through the driving groove 61 and is fixedly connected to the bottom of the bearing platform 3, and the side wall of the driven shaft 65 is fixedly connected with a driven gear 66, the driven gear 66 is meshed with the driving gear 62, and the bottom of the inner cavity of the base 1 is fixedly connected with a first motor 67, and the output shaft of the first motor 67 is connected to the driving shafts 6 on both sides through a pulley group 68.

[0035] Through the arrangement of the above structure, the first motor 67 is turned on to drive the driving shafts 6 on both sides to rotate in the same direction simultaneously through the pulley set 68. The driven gear 66 meshes with the driving gear 62, so that the driven slip ring 64 slides with the driven shaft 65 on the positioning slip ring 63, thereby driving the supporting platform 3 to rotate periodically, causing the culture dish 4 fixed on the supporting platform 3 to rotate and shake, so that the stem cells in the culture dish 4 are evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of stem cells and improving the culture efficiency.

[0036] Reference Figure 2 , Figure 4 , Figure 5 and Figure 6 , wherein a heating groove 31 is provided in the bearing platform 3, the top of the driven slip ring 64 fits and slides with the top of the inner cavity of the base 1, a heat conduction groove 32 is provided on the side wall of the heating groove 31, and the other end of the heat conduction groove 32 is connected with the inner cavity of the driving groove 61, and sealing rings 33 are fixedly connected on both sides of the base 1, the inner diameter of the sealing ring 33 is larger than the driving groove 61 and coincides with its central axis, and the top of the sealing ring 33 fits and slides with the bottom of the bearing platform 3, so that the high humidity environment in the sealing chamber 2 can be effectively prevented from being transmitted to the base 1, so as to protect the installation structure in the base 1 and improve the durability of the device, a temperature sensor is provided in the heating groove 31, and the temperature sensor adopts the existing mature foundation, and the specific model is SY-SRT0113, and a solenoid valve is provided in the heat conduction groove 32, and the temperature sensor and the solenoid valve are electrically connected;

[0037] By setting the above structure, during the rotation of the driven slip ring 64 and the meshing rotation of the driven gear 66 and the driving gear 62, heat will be generated in the driving groove 61 due to friction, and this part of the heat will enter the heating groove 31 along the heat conducting groove 32, so as to heat the culture dish 4 from the bottom thereof, and keep the culture dish 4 in a temperature environment suitable for the growth of stem cells (36.5-37.5 degrees), and when the temperature in the heating groove 31 is lower than the above temperature, the solenoid valve in the heat conducting groove 32 will be opened to heat the culture dish 4, and when the temperature in the heating groove 31 is higher than the above temperature, the solenoid valve in the heat conducting groove 32 will be closed to stop heating the culture dish 4, so as to achieve effective control of the culture temperature, improve the culture effect of stem cells, and utilize the heat generated during the working process, which is more energy-saving and environmentally friendly.

[0038] Reference Figure 2 , Figure 5 and Figure 6 , wherein the clamping part comprises two clamping plates 7, which are arranged on both sides of the bearing platform 3 and slide along the long axis direction of the clamping plates 7, the top of the clamping plates 7 is arranged with an arc chamfer and its side wall is provided with a clamping arc groove 71, both sides of the bearing platform 3 are provided with a clamping slide groove 72, the bottom of the clamping plate 7 is slidably connected with the inner wall of the clamping slide groove 72, a guide slide rod 73 is fixedly connected in the clamping slide groove 72, the guide slide rod 73 passes through the side wall of the clamping plate 7, and a clamping spring 74 is sleeved on the outer wall of the guide slide rod 73;

[0039] Through the arrangement of the above structure, the culture dish 4 is inserted from the top of the two clamping plates 7, and the side walls of the culture dish 4 are used to squeeze the clamping plates 7, so that the clamping spring 74 is compressed. When the culture dish 4 is fully inserted, the clamping spring 74 rebounds to stably clamp it between the clamping plates 7 on both sides, thereby improving the stability of the culture dish 4 during the culture process.

[0040] Reference Figure 5 , Figure 7-Figure 9, wherein the humidifying part includes a humidifying box 8, which is fixedly connected to the bottom of the planetary gear disk 5, and a rotating shaft 81 is rotatably connected to the bottom of the humidifying box 8. A second motor 82 is fixedly connected to the top of the inner cavity of the humidifying box 8, and an arc-shaped baffle 83 is fixedly connected to the humidifying box 8. The top of the rotating shaft 81 passes through the arc-shaped baffle 83 and is fixedly connected to the output end of the second motor 82. A lifting slider 84 is slidably sleeved on the rotating shaft 81, and a cutting blade 841 is fixedly connected to the outer wall of the lifting slider 84. A spray hole 85 is provided on the side wall of the humidifying box 8, and the spray hole 85 is connected to the bottom cavity of the humidifying box 8. A linkage shaft 671 is fixedly connected to the output end of the first motor 67, and the top of the linkage shaft 671 passes through and extends into the planetary gear disk 5 and is fixedly connected to a linkage gear 672, which is meshed with the planetary gear disk 5; a limited sliding groove 811 is provided on the side wall of the rotating shaft 81, and the protrusion on the inner wall of the lifting slider 84 is inserted into the limited sliding groove 811 and slidably connected therewith. Next, multiple groups of spring telescopic rods 86 are fixedly connected between the lifting slider 84 and the arc-shaped baffle 83, preferably three groups here, a piston sleeve 87 is fixedly connected to the upper part of the side wall of the rotating shaft 81, a limit ring 871 is fixedly connected inside the piston sleeve 87, a piston slider 872 is slidably connected inside the piston sleeve 87, a lifting spring 873 is fixedly connected between the piston slider 872 and the limit ring 871, a through-groove 874 is provided at the bottom of the piston sleeve 87, a pull rope 88 is fixedly connected to the top of the lifting slider 84, the pull rope 88 passes through the through-groove 874, the limit ring 871 and the middle part of the lifting spring 873 in sequence and is fixedly connected to the side wall of the piston slider 872, a magnetic block 89 is fixedly connected to the inner wall of the humidifying box 8, the magnetic block 89 and the piston slider 872 repel each other, a humidity sensor is fixedly connected to the bottom of the humidifying box 8, and the humidity sensor is electrically connected to the second motor 82, and the humidity sensor adopts the existing mature technology, and the specific model is SSHT40;

[0041] It should be noted that the centrifugal force generated by the piston slider 872 during rotation is greater than the pressure required for the three sets of spring telescopic rods 86 to retract, and the repulsive force between the magnetic block 89 and the piston slider 872 is greater than the centrifugal force generated by the piston slider 872 during rotation;

[0042] Through the arrangement of the above structure, the first motor 67 will drive the linkage shaft 671 to rotate, so that the linkage gear 672 and the planetary gear disc 5 will mesh and rotate, thereby driving the planetary gear disc 5 to rotate, so that the humidification box 8 rotates in the sealed chamber 2, and the humidity sensor will detect the humidity in the humidification box 8 in real time. When the humidity does not meet the preset range of the cultivation setting (90-95%), the humidity sensor will transmit a start signal to the second motor 82, and the second motor 82 will drive the rotating shaft 81 to rotate, so that the cutting flow blade 841 rotates at the bottom of the humidification box 8. At this time, under the action of centrifugal force, the piston slider 872 will slide to one side of the stretched lifting spring 873, and thereby pull the pull rope 88 upward, so that the lifting slider 84 brings the cutting flow blade 841 out of the clear water surface, and during the rotation process, the piston sleeve 87 will move to the magnetic block 89, and the magnetic block 89 will The piston slider 872 is magnetically repelled by the piston slider 872, and the rebound effect of the lifting spring 873 causes the piston slider 872 to move to the side away from the magnetic block 89, thereby preventing the pull rope 88 from being loosened, and under the rebound effect of the multiple sets of spring telescopic rods 86, the lifting slider 84 and the cutting flow blade 841 are re-sunk into the clean water, and so on. In the process of the cutting flow blade 841 constantly going out of and into the clean water, the clean water will be slapped and cut to form fine mist-like droplets, and after the cutting flow blade 841 goes out of the clean water surface, an upward thrust will be generated, so that the above-produced mist-like droplets are discharged from the spray hole 85, and accompanied by the overall rotation effect of the humidification box 8, the atomization humidification in the sealed chamber 2 is fully realized, and then when the humidity in the sealed chamber 2 reaches the appropriate threshold range, the second motor 82 will stop rotating, thereby ensuring a suitable humidity environment for stem cell culture and improving the culture effect.

[0043] Reference Figure 1-Figure 3 and Figure 5 , wherein a sealing groove 9 is provided between the sealing chamber 2 and the base 1, a sealing plate 91 is slidably connected in the sealing groove 9, a locking sleeve 92 is rotatably connected to the top of the sealing chamber 2, a locking slider 93 is slidably connected inside the locking sleeve 92, a locking spring 94 is fixedly connected between the locking slider 93 and the inner wall of the locking sleeve 92, when the locking spring 94 is naturally stretched, the distance from the end of the locking slider 93 to the rotation axis of the locking sleeve 92 is greater than the distance from the side wall of the sealing plate 91 to the rotation axis of the locking sleeve 92, and when the sealing plate 91 is fully inserted into the sealing groove 9, the top of the sealing plate 91 fits with the bottom of the locking slider 93;

[0044] By setting the above structure, the locking slider 93 is rotated to release the squeezing of the side wall of the sealing plate 91. At this time, the sealing plate 91 will fall along the sealing groove 9 until the sealing chamber 2 is completely sealed. Then the locking slider 93 is rotated again. Under the rebound action of the locking spring 94, the overall length of the locking slider 93 and the locking sleeve 92 will be greater than the length when it was squeezed and locked before. At this time, the locking slider 93 is rotated and allowed to stay on the top of the sealing plate 91 to achieve the locking and blocking of the sealing plate 91, thereby ensuring the sealing effect, facilitating the opening and closing of the sealing chamber 2, and improving the convenience of storing and accessing the culture dish 4.

[0045] Reference Figure 1 , Figure 5 , wherein, both sides of the top of the side wall of the sealed chamber 2 are fixedly connected with an exhaust pipe 21, and the side wall of the sealed chamber 2 is fixedly connected with a gas pump 22, the output end of the gas pump 22 is connected with the inner cavity of the sealed chamber 2, and the input end of the gas pump 22 is connected with an external carbon dioxide gas source, and electromagnetic valves are arranged in the exhaust pipe 21 and the input end of the gas pump 22, and a carbon dioxide gas detector is arranged in the sealed chamber 2, and the carbon dioxide gas detector belongs to the existing mature technology, and the specific model is GTYQ-STC20;

[0046] Through the arrangement of the above structure, the gas pump 22 is turned on, and carbon dioxide gas will be filled into the bottom of the inner cavity of the sealed chamber 2, and the original gas in the sealed chamber 2 will be discharged along the exhaust pipe 21 to achieve air pressure balance. When the carbon dioxide gas detector in the exhaust pipe 21 detects that the carbon dioxide concentration meets the culture requirements (preferably 5%), the gas pump 22 and its input end and the electromagnetic valve in the exhaust pipe 21 are immediately closed to simulate the gas environment in the sealed chamber 2, thereby improving the environment suitable for stem cell culture and ensuring the culture effect of stem cells. When the supporting platform 3 rotates, the gases in the sealed chamber 2 will be quickly mixed, so that the carbon dioxide gas detector can quickly and accurately detect carbon dioxide.

[0047] Reference Figure 1-Figure 9In the present invention, when in use, the culture dish 4 containing stem cells and nutrients required for culture is inserted from the top of the two clamping plates 7, so that the side walls of the culture dish 4 are used to squeeze the clamping plates 7, so that the clamping springs 74 are compressed. When the culture dish 4 is fully inserted, the clamping springs 74 are used to stably clamp the culture dish 4 between the clamping plates 7 on both sides, thereby improving the stability of the culture dish 4 during the culture process; then the locking slider 93 is rotated to release the squeezing of the side walls of the sealing plate 91, and the sealing plate 91 will fall along the sealing groove 9 until the sealing chamber 2 is completely sealed, and then the locking slider 93 is rotated again, and the locking slider 93 and the locking sleeve are locked together under the rebound effect of the locking spring 94. The overall length of the cylinder 92 will be greater than the length when it was squeezed and locked before. At this time, the locking slider 93 is rotated and stopped on the top of the sealing plate 91 to achieve the locking and blocking of the sealing plate 91, ensuring the sealing effect; then the gas pump 22 is turned on, and carbon dioxide gas will be filled into the bottom of the inner cavity of the sealed chamber 2, and the original gas in the sealed chamber 2 will be discharged along the exhaust pipe 21 to achieve air pressure balance. When the carbon dioxide gas detector in the exhaust pipe 21 detects that the carbon dioxide concentration meets the culture requirements, the gas pump 22 and its input end and the electromagnetic valve in the exhaust pipe 21 are immediately closed to simulate the gas environment in the sealed chamber 2, improve the environment suitable for stem cell culture, and ensure the culture effect of stem cells;

[0048] After the sealed chamber 2 is closed, the first motor 67 is turned on to drive the driving shafts 6 on both sides to rotate in the same direction simultaneously through the pulley set 68. The driven gear 66 meshes with the driving gear 62, so that the driven slip ring 64 slides with the driven shaft 65 on the positioning slip ring 63, thereby driving the supporting platform 3 to rotate periodically, so that the culture dish 4 fixed on the supporting platform 3 rotates and shakes, so that the stem cells in the culture dish 4 are evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of stem cells and improving the culture efficiency; and when the supporting platform 3 rotates, the gases in the sealed chamber 2 will be quickly mixed, so that the carbon dioxide gas detector can quickly and accurately detect carbon dioxide. In the process of rotation of the driven slip ring 64 and meshing rotation of the driven gear 66 and the driving gear 62, heat will be generated in the driving groove 61 due to friction, and this part of the heat will enter the heating groove 31 along the heat-conducting groove 32, so as to heat the culture dish 4 from the bottom thereof, and keep the culture dish 4 in a temperature environment suitable for the growth of stem cells, and when the temperature in the heating groove 31 is lower than the above temperature, the solenoid valve in the heat-conducting groove 32 will be opened to heat the culture dish 4, and when the temperature in the heating groove 31 is higher than the above temperature, the solenoid valve in the heat-conducting groove 32 will be closed to stop heating the culture dish 4, so as to effectively control the culture temperature and improve the culture effect of stem cells;

[0049] In addition, the first motor 67 will also drive the linkage shaft 671 to rotate, so that the linkage gear 672 and the planetary gear plate 5 will mesh and rotate, thereby driving the planetary gear plate 5 to rotate, so that the humidification box 8 rotates in the sealed chamber 2. At this time, the humidity sensor will detect the humidity in the humidification box 8 in real time. When the humidity does not meet the preset range of the cultivation setting, the humidity sensor will transmit a start signal to the second motor 82. At this time, the second motor 82 will drive the rotating shaft 81 to rotate, so that the cutting flow blade 841 rotates at the bottom of the humidification box 8. At this time, under the action of centrifugal force, the piston slider 872 will slide to the side of the stretched lifting spring 873, and thereby pull the pull rope 88 upward, so that the lifting slider 84 brings the cutting flow blade 841 out of the clear water surface, and during the rotation process, the piston sleeve 87 will move to the magnetic block 89. At this time, the magnetic block 89 will engage with the piston slider 872. The magnetic repulsion occurs between them, and the rebound effect of the lifting spring 873 causes the piston slider 872 to move to the side away from the magnetic block 89, thereby preventing the pull rope 88 from being loosened, and under the rebound effect of the multiple sets of spring telescopic rods 86, the lifting slider 84 and the cutting flow blade 841 are re-sunk into the clean water, and so on. In the process of the cutting flow blade 841 constantly going out of and into the clean water, the clean water will be slapped and cut to form fine mist-like droplets, and after the cutting flow blade 841 goes out of the clean water surface, an upward thrust will be generated, so that the above-mentioned mist-like droplets are discharged from the spray hole 85, and accompanied by the overall rotation effect of the humidification box 8, the atomization humidification in the sealed chamber 2 is fully realized, and then when the humidity in the sealed chamber 2 reaches the appropriate threshold range, the second motor 82 will stop rotating, thereby ensuring a suitable humidity environment for stem cell culture and improving the culture effect.

[0050] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A stem cell culture device, comprising a base (1) and a sealed chamber (2) fixed on the top thereof, characterized in that: Also includes: A support platform (3) is rotatably connected to the sealed chamber (2); a shaking part for driving the support platform (3) to rotate is arranged in the base (1); the shaking part comprises two driving shafts (6); driving grooves (61) are provided on both sides of the top of the base (1); the bottom ends of the driving shafts (6) rotate with the bottom of the inner cavity of the base (1); the top ends of the two driving shafts (6) extend to the inner cavities of the driving grooves (61) on both sides respectively and are fixedly connected to driving gears (62); the outer wall of the driving shaft (6) is rotatably connected to A positioning slip ring (63), the positioning slip ring (63) being fixedly connected to the side wall of the inner cavity of the base (1), a driven slip ring (64) being slidably sleeved on the top of the positioning slip ring (63), a driven shaft (65) being fixedly connected to the top of the driven slip ring (64), the driven shaft (65) passing through the driving groove (61) and being fixedly connected to the bottom of the supporting platform (3), and a driven gear (66) being fixedly connected to the side wall of the driven shaft (65), the driven gear (66) being meshingly connected to the driving gear (62); A heating groove (31) is provided in the support platform (3), the top of the driven slip ring (64) is fitted and slidably connected to the top of the inner cavity of the base (1), a heat conduction groove (32) is provided on the side wall of the heating groove (31), the other end of the heat conduction groove (32) is connected to the inner cavity of the driving groove (61), sealing rings (33) are fixedly connected to both sides of the base (1), the inner diameter of the sealing ring (33) is larger than that of the driving groove (61) and coincides with its central axis, and the top of the sealing ring (33) is fitted and slidably connected to the bottom of the support platform (3), a temperature sensor is provided in the heating groove (31), an electromagnetic valve is provided in the heat conduction groove (32), and the temperature sensor and the electromagnetic valve are electrically connected. Wherein, a culture dish (4) is placed on the carrying platform (3), and clamping parts for fixing the culture dish (4) are arranged on both sides of the carrying platform (3); A planetary gear disc (5) is rotatably connected to the top of the inner cavity of the sealed chamber (2); a humidifying portion for regulating the humidity in the sealed chamber (2) is provided at the bottom of the planetary gear disc (5); and the planetary gear disc (5) is driven to rotate by the shaking portion; The humidifying section comprises a humidifying box (8), the humidifying box (8) being fixedly connected to the bottom of the planetary gear disk (5), the bottom of the humidifying box (8) being rotatably connected to a rotating shaft (81), the top of the inner cavity of the humidifying box (8) being fixedly connected to a second motor (82), the inner cavity of the humidifying box (8) being fixedly connected to an arc-shaped baffle (83), the top of the rotating shaft (81) passing through the arc-shaped baffle (83) and being fixedly connected to an output end of the second motor (82), a lifting slider (84) being slidably sleeved on the rotating shaft (81), a cutting blade (841) being fixedly connected to an outer wall of the lifting slider (84), a spray hole (85) being provided on a side wall of the humidifying box (8), and the spray hole (85) being in communication with the bottom cavity of the humidifying box (8); The side wall of the rotating shaft (81) is provided with a limit slide groove (811), and the protrusion on the inner wall of the lifting slider (84) is inserted into the limit slide groove (811) and is slidably connected thereto. A plurality of groups of spring telescopic rods (86) are fixedly connected between the lifting slider (84) and the arc-shaped baffle (83). A piston sleeve (87) is fixedly connected to the upper part of the side wall of the rotating shaft (81). A limit ring (871) is fixedly connected inside the piston sleeve (87). A piston slider (872) is slidably connected inside the piston sleeve (87). The piston slider (87) A lifting spring (873) is fixedly connected between the piston sleeve (87) and the limiting ring (871), a through-connection groove (874) is provided at the bottom of the piston sleeve (87), a pull rope (88) is fixedly connected to the top of the lifting slider (84), the pull rope (88) passes through the through-connection groove (874), the limiting ring (871) and the middle of the lifting spring (873) in sequence and is fixedly connected to the side wall of the piston slider (872), a magnetic block (89) is fixedly connected to the inner wall of the humidifying box (8), and the magnetic block (89) and the piston slider (872) repel each other magnetically; The bottom of the humidifying box (8) is fixedly connected to a humidity sensor, and the humidity sensor is electrically connected to the second motor (82).

2. A stem cell culture device according to claim 1, characterized in that: A first motor (67) is fixedly connected to the bottom of the inner cavity of the base (1); an output shaft of the first motor (67) is transmission-connected to the drive shafts (6) on both sides via a pulley set (68); an output end of the first motor (67) is fixedly connected to a linkage shaft (671); a top end of the linkage shaft (671) extends through the planetary gear disk (5) and is fixedly connected to a linkage gear (672); the linkage gear (672) is meshingly connected to the planetary gear disk (5).

3. A stem cell culture device according to claim 1, characterized in that: The clamping part comprises two clamping plates (7), the two clamping plates (7) are arranged on both sides of the supporting platform (3) and slide along the long axis direction of the clamping plates (7), the top of the clamping plate (7) is arranged with an arc chamfer and its side wall is provided with a clamping arc groove (71), both sides of the supporting platform (3) are provided with a clamping slide groove (72), the bottom of the clamping plate (7) is slidably connected to the inner wall of the clamping slide groove (72), a guide slide rod (73) is fixedly connected in the clamping slide groove (72), the guide slide rod (73) passes through the side wall of the clamping plate (7), and the outer wall of the guide slide rod (73) is sleeved with a clamping spring (74).

4. The stem cell culture device according to claim 1, characterized in that: A sealing groove (9) is provided between the sealing chamber (2) and the base (1), a sealing plate (91) is slidably connected in the sealing groove (9), a locking sleeve (92) is rotatably connected to the top of the sealing chamber (2), a locking slider (93) is slidably connected inside the locking sleeve (92), and a locking spring (94) is fixedly connected between the locking slider (93) and the inner wall of the locking sleeve (92).

5. The stem cell culture device according to claim 1, characterized in that: Exhaust pipes (21) are fixedly connected to both sides of the top of the side wall of the sealed chamber (2), and a gas pump (22) is fixedly connected to the side wall of the sealed chamber (2). The output end of the gas pump (22) is connected to the inner cavity of the sealed chamber (2), and the input end of the gas pump (22) is connected to an external carbon dioxide gas source. Solenoid valves are provided in the exhaust pipe (21) and the input end of the gas pump (22).

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