A method for extracting anti-wrinkle complex

Abstract

The present invention relates to the technical field of skin care product raw materials, and specifically discloses a method for extracting an anti-wrinkle complex, comprising the following steps: step 1), mixing oats, corn, soybeans, wolfberries, peas, and water, heating to 45-55°C to obtain a premix; step 2), adding a composite enzyme to the premix, constant temperature 45-55°C, stirring for 48-56h; step 3), heating to kill enzymes, cooling; step 4), cooling to 30-35°C, adding composite bacteria, constant temperature 30-35°C, and fermenting for 72-80h; step 5), heating and sterilizing, cooling; step 6), filtering, taking filtrate, and obtaining an anti-wrinkle complex; the composite enzyme is a composite of glucanase, neutral protease, and papain; the composite bacteria is a composite of Staphylococcus aureus and Aspergillus cristatus. The present invention has the advantage of rapid wrinkle removal.

Description

Technical Field

[0001] The invention relates to the field of skin care product raw materials, in particular to a method for extracting an anti-wrinkle complex. Background Art

[0002] Skin care products are products for skin care. With the development of science and technology, the production cost of skin care products has dropped significantly, making skin care products one of the popular consumer products. By using skin care products to care for the skin, it can improve the health of the skin, thereby greatly improving people's spirit and temperament, and is deeply loved by consumers.

[0003] With the development of technology, skin care products have more and more functions and are becoming more targeted. By using skin care products with specific functions for skin care, the skin can be repaired in a targeted manner, thereby better improving people's spirit and temperament. Among them, skin aging and wrinkles are the most common skin problems that consumers most want to repair. Therefore, some skin care products add anti-wrinkle and anti-aging ingredients to reduce skin wrinkles and restore the skin to youthfulness.

[0004] However, although existing skin care products with anti-wrinkle and anti-aging effects can effectively delay aging, they are slow to take effect in smoothing wrinkles, resulting in a relatively general effect of restoring youth. Long-term use of the products will show certain effects, which is difficult to meet the needs of consumers who want to quickly smooth wrinkles to restore youth. Therefore, there is still room for improvement. Summary of the invention

[0005] In order to achieve the effect of quickly removing wrinkles, the present application provides a method for extracting an anti-wrinkle complex.

[0006] The present application provides an anti-wrinkle compound extraction method using the following technical solution:

[0007] A method for extracting an anti-wrinkle compound comprises the following steps:

[0008] Step 1), mixing oats, corn, soybeans, wolfberries, peas and water, and heating to 45-55° C. to obtain a premix;

[0009] Step 2), adding complex enzyme to the premix, maintaining the temperature at 45-55°C, and stirring for 48-56 hours;

[0010] Step 3), heating to inactivate the enzyme and cooling down;

[0011] Step 4), cool down to 30-35°C, add compound bacteria, keep constant temperature at 30-35°C, and ferment for 72-80h;

[0012] Step 5), heating and sterilization, cooling;

[0013] Step 6), filtering and taking the filtrate to obtain an anti-wrinkle composite;

[0014] The complex enzyme is a combination of glucanase, neutral protease and papain;

[0015] The composite bacteria is a combination of Staphylococcus capitis and Aspergillus cristatus.

[0016] By adopting the above technical scheme, by first using a composite enzyme for preliminary enzymatic hydrolysis and then using a composite bacteria for fermentation, the molecular weight of the extract is made to be 1000-3000Da, and the fermentation product is easily absorbed by the skin. Under the combination of the active ingredients of oats, corn, soybeans, wolfberries, and peas, the activity of skin cells can be well activated, so that the ability of the skin to synthesize collagen is significantly restored, thereby greatly increasing the amount of collagen produced in the skin, thereby well resisting the loss of collagen, and quickly filling the collagen in the skin, thereby restoring the skin skeleton to support the skin, and repairing wrinkles and other collapsed areas, achieving the effect of quickly smoothing wrinkles, making the skin quickly regain its youthfulness, and making the skin elastic and full of luster, which well meets the needs of consumers for quick wrinkle removal.

[0017] Preferably, in step 1), the mass ratio of oats, corn, soybeans, wolfberries and peas is 4-6:4-6:4-6:0.5-1.5:0.5-1.5.

[0018] By adopting the above technical scheme and specifically selecting the mass ratio of oats, corn, soybeans, wolfberries and peas, the active ingredients of oats, corn, soybeans, wolfberries and peas can better cooperate with each other, thereby achieving a faster anti-wrinkle effect, quickly smoothing skin wrinkles and quickly restoring the skin to youthfulness.

[0019] Preferably, in step 1), the mass ratio of oats, corn, soybeans, wolfberries, peas and water is 4-6:4-6:4-6:0.5-1.5:0.5-1.5:70-80.

[0020] By adopting the above technical scheme and specifically selecting the mass ratio of oats, corn, soybeans, wolfberries, peas and water, the extract can be better extracted into water, the extraction efficiency is high, the cost is well controlled, and the economic value is high.

[0021] Preferably, in the complex enzyme, the mass ratio of glucanase, neutral protease and papain is 0.2-0.4:0.1-0.3:0.1-0.3.

[0022] By adopting the above technical solution and specifically selecting the mass ratio of glucanase, neutral protease and papain, the enzymatic hydrolysis effect is better, the activity of the extract is better activated, the skin improvement effect is better, and wrinkles are smoothed more quickly.

[0023] Preferably, in step 2), the ratio of the mass of the added complex enzyme to the mass of the added water in step 1) is 0.4-1:70-80.

[0024] By adopting the above technical solution, a good enzymatic hydrolysis effect is achieved, while the cost is effectively controlled, and the economic value is relatively high.

[0025] Preferably, in the composite bacteria, the mass ratio of Staphylococcus capitis to Aspergillus cristatus is 0.1-0.3:0.5-0.7.

[0026] By adopting the above technical scheme and specifically selecting the mass ratio of Staphylococcus capitis and Aspergillus cristatus, the fermentation effect is better, and the activity of the extract is better improved, so that the extract can improve the skin faster and remove wrinkles more efficiently, thereby better meeting consumers' needs for rapid wrinkle removal.

[0027] Preferably, in step 4), the ratio of the mass of the added composite bacteria to the mass of the added water in step 1) is 0.6-1:70-80.

[0028] By adopting the above technical solution, a better fermentation effect is achieved while the cost is well controlled, thus having a higher economic value.

[0029] Preferably, in step 1), oats, corn, soybeans, wolfberries and peas are first crushed to 60-100 meshes and then added into water for mixing.

[0030] By adopting the above technical solution, the ingredients in oats, corn, soybeans, wolfberries and peas are easier to extract, the extraction efficiency is higher, and the process cost is effectively reduced.

[0031] Preferably, in step 3), the specific step of heating to inactivate the enzyme is: heating to 85-90° C. and keeping warm for 30-40 minutes.

[0032] By adopting the above technical solution, the enzyme inactivation effect is better and the product safety is higher.

[0033] Preferably, in step 5), the specific steps of heating and sterilizing are: heating to 90-95° C. and keeping warm for 30-40 minutes.

[0034] By adopting the above technical solution, the sterilization effect is better and the product safety is higher.

[0035] In summary, this application has the following beneficial effects:

[0036] 1. Since the present application uses a composite enzyme for preliminary enzymatic hydrolysis and then uses a composite bacteria for fermentation, the molecular weight of the extract is 1000-3000Da, and the fermentation product is easily absorbed by the skin. The active ingredients of oats, corn, soybeans, wolfberries, and peas are compounded with each other, which can well activate the activity of skin cells, so that the ability of the skin to synthesize collagen is significantly restored, thereby greatly increasing the amount of collagen produced in the skin, thereby well resisting the loss of collagen, and quickly filling the skin with collagen, thereby restoring the skin skeleton to support the skin, and repairing wrinkles and other collapsed areas, achieving the effect of quickly smoothing wrinkles, making the skin quickly regain its youthfulness, and making the skin elastic and full of luster, which well meets the needs of consumers for rapid wrinkle removal.

[0037] 2. In the present application, it is preferred to specifically select the mass ratio of oats, corn, soybeans, wolfberries and peas so that the active ingredients of oats, corn, soybeans, wolfberries and peas can better cooperate with each other, thereby achieving a faster anti-wrinkle effect, so that skin wrinkles are quickly smoothed and the skin quickly regains its youthfulness.

[0038] 3. In the present application, it is preferred to specifically select the mass ratio of glucanase, neutral protease and papain so as to achieve better enzymatic hydrolysis effect, better activate the activity of the extract, better improve the skin effect, and better and faster smooth wrinkles.

[0039] 4. In the present application, it is preferred to specifically select the mass ratio of Staphylococcus capitis and Aspergillus cristatus to achieve a better fermentation effect and better improve the activity of the extract, so that the extract can improve the skin faster and remove wrinkles more efficiently, thereby better meeting consumers' demand for rapid wrinkle removal. DETAILED DESCRIPTION

[0040] The present application is further described in detail below with reference to the embodiments.

[0041] Example 1

[0042] A method for extracting an anti-wrinkle compound comprises the following steps:

[0043] Step 1), 4kg oats, 4kg corn, 4kg soybeans, 0.5kg wolfberry, and 0.5kg peas are crushed to 60 mesh, and then put into a reactor, 70kg water is added, and heated to 45° C. to obtain a premix.

[0044] Step 2), add 0.4 kg of complex enzyme to the premix, keep the temperature at 45°C, rotate at 30 r / min, and stir for 48 hours.

[0045] Step 3), heating to 85°C to inactivate the enzyme, keeping the temperature for 30 min, and cooling down.

[0046] Step 4), cool to 30°C, add 0.6 kg of composite bacteria, keep constant temperature at 30°C, and ferment for 72 hours.

[0047] Step 5), heat sterilize, heat to 90°C, keep warm for 30 minutes, and cool down.

[0048] Step 6), filtering through a 200-mesh filter cloth, taking the filtrate to obtain an anti-wrinkle composite.

[0049] Among them, oats were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0050] Among them, the corn was purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0051] Among them, soybeans were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0052] Among them, wolfberries were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0053] Among them, peas were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0054] The composite enzyme is a combination of glucanase, neutral protease and papain, and the mass ratio of glucanase, neutral protease and papain is 0.2:0.1:0.1.

[0055] Glucanase was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0056] Neutral protease was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0057] Papain was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0058] Among them, the composite bacteria is a combination of Staphylococcus capitis and Aspergillus cristatus, and the mass ratio of Staphylococcus capitis and Aspergillus cristatus is 0.1:0.5.

[0059] Staphylococcus aureus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-85755.

[0060] Aspergillus cristatus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-104127.

[0061] Example 2

[0062] A method for extracting an anti-wrinkle compound comprises the following steps:

[0063] Step 1), 5kg of oats, 5kg of corn, 5kg of soybeans, 1kg of wolfberry, and 1kg of peas are crushed to 80 meshes, and then put into a reactor, 75kg of water is added, and heated to 50° C. to obtain a premix.

[0064] Step 2), add 0.7 kg of complex enzyme to the premix, keep the temperature at 50°C, rotate at 30 r / min, and stir for 52 hours.

[0065] Step 3), heating to 88°C to inactivate the enzyme, keeping the temperature for 35 minutes, and cooling down.

[0066] Step 4), cool to 32°C, add 0.8 kg of composite bacteria, keep constant temperature at 32°C, and ferment for 76 hours.

[0067] Step 5), heat sterilize, heat to 92°C, keep warm for 35 minutes, and cool down.

[0068] Step 6), filtering through a 200-mesh filter cloth, taking the filtrate to obtain an anti-wrinkle composite.

[0069] Among them, oats were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0070] Among them, the corn was purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0071] Among them, soybeans were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0072] Among them, wolfberries were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0073] Among them, peas were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0074] The composite enzyme is a combination of glucanase, neutral protease and papain, and the mass ratio of glucanase, neutral protease and papain is 0.3:0.2:0.2.

[0075] Glucanase was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0076] Neutral protease was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0077] Papain was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0078] Among them, the composite bacteria is a combination of Staphylococcus capitis and Aspergillus cristatus, and the mass ratio of Staphylococcus capitis and Aspergillus cristatus is 0.2:0.6.

[0079] Staphylococcus aureus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-85755.

[0080] Aspergillus cristatus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-104127.

[0081] Example 3

[0082] A method for extracting an anti-wrinkle compound comprises the following steps:

[0083] Step 1), 6 kg of oats, 6 kg of corn, 6 kg of soybeans, 1.5 kg of wolfberry, and 1.5 kg of peas are crushed to 100 mesh, and then put into a reactor, 80 kg of water is added, and heated to 55° C. to obtain a premix.

[0084] Step 2), add 1 kg of complex enzyme to the premix, keep the temperature at 55°C, rotate at 30 r / min, and stir for 56 hours.

[0085] Step 3), heating to 90°C to inactivate the enzyme, keeping the temperature for 40 minutes, and cooling down.

[0086] Step 4), cool down to 35°C, add 1 kg of composite bacteria, keep constant temperature at 35°C, and ferment for 80 hours.

[0087] Step 5), heat sterilize, heat to 95°C, keep warm for 40 minutes, and cool down.

[0088] Step 6), filtering through a 200-mesh filter cloth, taking the filtrate to obtain an anti-wrinkle composite.

[0089] Among them, oats were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0090] Among them, the corn was purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0091] Among them, soybeans were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0092] Among them, wolfberries were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0093] Among them, peas were purchased from Wuhan Xinfengshuo Trading Co., Ltd.

[0094] The composite enzyme is a combination of glucanase, neutral protease and papain, and the mass ratio of glucanase, neutral protease and papain is 0.4:0.3:0.3.

[0095] Glucanase was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0096] Neutral protease was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0097] Papain was purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0098] Among them, the composite bacteria is a combination of Staphylococcus capitis and Aspergillus cristatus, and the mass ratio of Staphylococcus capitis and Aspergillus cristatus is 0.3:0.7.

[0099] Staphylococcus aureus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-85755.

[0100] Aspergillus cristatus was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-104127.

[0101] Comparative Example 1

[0102] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0103] In the complex enzyme, pectinase is used to replace glucanase in equal amounts.

[0104] Pectinase was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0105] Comparative Example 2

[0106] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0107] In the complex enzyme, serrapeptase is used to replace neutral protease in equal amounts.

[0108] Serrapeptase was purchased from Guangdong Ousman Biotechnology Co., Ltd.

[0109] Comparative Example 3

[0110] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0111] In the complex enzyme, xylanase is used to replace papain in equal amounts.

[0112] Xylanase was purchased from Shangdong Pingju Biotechnology Co., Ltd.

[0113] Comparative Example 4

[0114] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0115] In the complex enzyme, pectinase is used to replace glucanase in equal amount; serrapeptase is used to replace neutral protease in equal amount; and xylanase is used to replace papain in equal amount.

[0116] Pectinase was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0117] Serrapeptase was purchased from Guangdong Ousman Biotechnology Co., Ltd.

[0118] Xylanase was purchased from Shangdong Pingju Biotechnology Co., Ltd.

[0119] Comparative Example 5

[0120] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0121] In the composite bacteria, Bifidobacterium breve was used to replace Staphylococcus capitis in equal amounts.

[0122] Bifidobacterium breve was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67242.

[0123] Comparative Example 6

[0124] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0125] In the composite bacteria, Lactobacillus plantarum was used to replace Aspergillus cristatus in equal amounts.

[0126] Bacillus subtilis was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-59174.

[0127] Comparative Example 7

[0128] A method for extracting an anti-wrinkle compound, compared with Example 2, the only difference is:

[0129] In the composite bacteria, Bifidobacterium breve was used to replace Staphylococcus capitis in equal amounts; and Bacillus subtilis was used to replace Aspergillus cristatus in equal amounts.

[0130] Bifidobacterium breve was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-67242.

[0131] Bacillus subtilis was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-59174.

[0132] Experiment 1

[0133] A number of volunteers, 35-40 years old females with facial wrinkles, were recruited. Ten volunteers were divided into groups and the test samples prepared from the anti-wrinkle composites of the embodiments and comparative examples were used in the groups.

[0134] Before starting the experiment, the skin wrinkle status of the volunteers was measured using the skin fast three-dimensional imaging system PRIMOS, which was recorded as the initial wrinkle measurement value. The initial wrinkle measurement values ​​of all volunteers were between 36 and 37.

[0135] The volunteers used the test samples once in the morning and once in the evening every day. After 30 days of using the test samples, the skin wrinkle status of the volunteers was measured again, which was recorded as the 30-day wrinkle measurement value.

[0136] During the experiment, a disposable face washcloth and clean water were used to wash the face. No other skin care products were used during the experiment. When using the test sample, 3 ml of the test sample was taken and applied evenly on the face.

[0137] When measuring the wrinkle status, the left and right eye corners, the middle of the forehead, and both cheeks are tested. The average of the five measurement values ​​is taken as the wrinkle measurement value, and the average value of each group of test data is taken.

[0138] Sample to be tested: Take 10g of the anti-wrinkle compound and 90g of deionized water, mix well to obtain the sample to be tested.

[0139] The specific test data of Experiment 1 are shown in Table 1.

[0140] Table 1

[0141]

[0142]

[0143] According to the data comparison in Table 1, the wrinkle reducing effect of the test samples prepared in each embodiment after 30 days of use is significantly higher than that of the test samples prepared in each comparative example. It can be seen that the anti-wrinkle complex prepared by the process of preliminary enzymatic hydrolysis using a composite enzyme composed of glucanase, neutral protease and papain and then fermentation using a composite bacteria composed of Staphylococcus capitis and Aspergillus crisscross can quickly repair the skin and quickly fill the collagen in the skin, thereby achieving the effect of rapid wrinkle removal.

[0144] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A method for extracting an anti-wrinkle compound, characterized in that: The following steps are involved: Step 1), oats, corn, soybeans, wolfberries, peas and water are mixed and heated to 45-55° C. to obtain a premix; Step 2), adding complex enzyme to the premix, maintaining the temperature at 45-55°C, and stirring for 48-56 hours; Step 3), heating to inactivate the enzyme and cooling down; Step 4), cool down to 30-35℃, add compound bacteria, keep constant temperature at 30-35℃, and ferment for 72-80h; Step 5), heating and sterilization, cooling; Step 6), filtering and taking the filtrate to obtain an anti-wrinkle composite; The complex enzyme is a combination of glucanase, neutral protease and papain; The composite bacteria is a combination of Staphylococcus capitis and Aspergillus cristatus; In the step 1), the mass ratio of oats, corn, soybeans, wolfberries and peas is 4-6:4-6:4-6:0.5-1.5:0.5-1.5; In the complex enzyme, the mass ratio of glucanase, neutral protease and papain is 0.2-0.4:0.1-0.3:0.1-0.3; In the composite bacteria, the mass ratio of Staphylococcus capitis to Aspergillus cristatus is 0.1-0.3:0.5-0.

7.

2. The method for extracting an anti-wrinkle compound according to claim 1, characterized in that: In the step 1), the mass ratio of oats, corn, soybeans, wolfberries, peas and water is 4-6:4-6:4-6:0.5-1.5:0.5-1.5:70-80.

3. The method for extracting an anti-wrinkle compound according to claim 1, characterized in that: In the step 2), the ratio of the added mass of the complex enzyme to the added mass of the water in the step 1) is 0.4-1:70-80.

4. The method for extracting an anti-wrinkle compound according to claim 1, characterized in that: In the step 4), the ratio of the mass of the added composite bacteria to the mass of the added water in the step 1) is 0.6-1:70-80.

5. The method for extracting an anti-wrinkle compound according to claim 1, characterized in that: In the step 1), oats, corn, soybeans, wolfberries and peas are first crushed to 60-100 meshes and then added into water for mixing.

6. The method for extracting an anti-wrinkle compound according to claim 1, characterized in that: In step 3), the specific steps of heating to inactivate the enzyme are: heating to 85-90° C. and keeping warm for 30-40 minutes.

7. The method for extracting an anti-wrinkle compound according to claim 1, characterized in that: In step 5), the specific steps of heating and sterilizing are: heating to 90-95° C. and keeping warm for 30-40 minutes.

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