A functional dressing based on egg white lysozyme and preparation method thereof

Abstract

The present invention relates to the field of biomedicine technology, and discloses a functional dressing based on egg white lysozyme and a preparation method thereof. The functional dressing based on egg white lysozyme comprises the following raw materials: hyaluronic acid, broad-spectrum lysozyme, multifunctional microcapsules, and deionized water; wherein the broad-spectrum lysozyme is formed by connecting egg white lysozyme and perillaldehyde through chemical bonding; and the multifunctional microcapsules are prepared by using carboxymethyl cellulose and lactoferrin as shells and embedding squalene as cores. The functional dressing prepared by the present invention has an excellent effect of promoting wound healing, can greatly reduce the healing time of the wound of the injured, has a broad-spectrum antibacterial property, can reduce the possibility of wound inflammation, does not need to be replaced even when used for a long time, effectively avoids secondary damage to the wound caused by frequent dressing changes, and alleviates the pain of the injured.

Description

Technical Field

[0001] The invention relates to the technical field of biomedicine, and in particular to a functional dressing based on egg white lysozyme and a preparation method thereof. Background Art

[0002] Dressings are common items in medical treatment and daily life, and play a very important role in wound treatment and infection prevention. Traditional dressings are directly covered on the wound surface to stop bleeding and isolate the external environment. Due to their limited hygroscopicity, they often need to be replaced, which can easily cause secondary damage to the wound, thereby delaying the healing time of the wound. Gel dressings have a network structure and good moisture absorption and moisture retention. They can provide a good healing environment for wound healing and will not adhere to the wound. They have become one of the preferred base materials in today's dressing preparation.

[0003] However, while gel dressings provide a moist cell repair environment for wounds, they also promote bacterial reproduction in a moist environment, leading to inflammation of the wound surface. Redness, swelling, and fever aggravate the pain of the injured and delay the healing process of the wound surface. Egg white lysozyme is a natural antibacterial protein that can effectively destroy bacterial cell walls, inhibit bacterial growth and reproduction, and has the function of promoting cell repair and regeneration, which helps wound healing. Using it to improve gel dressings can make the dressings have antibacterial ability and effectively reduce the occurrence of inflammation at the wound site. However, egg white lysozyme does not have broad-spectrum antibacterial properties and has poor antibacterial ability against Gram-negative bacteria, which limits its scope of use.

[0004] The patent with publication number CN109010896B discloses a water-resistant enhanced wound healing film and a preparation method thereof. The wound healing film with good water resistance, mechanical properties, antibacterial properties and protein absorption capacity is prepared by using eggshell, shell membrane, soluble mixed protein and lysozyme remaining in egg white. The patent exerts antibacterial effect through egg white lysozyme. In order to enhance the antibacterial ability of the wound healing film against Gram-negative bacteria, chitosan is used for auxiliary antibacterial effect, so that the wound healing film prepared by the patent has broad-spectrum antibacterial properties and can effectively avoid the occurrence of inflammation at the wound. However, the patent directly mixes the egg white lysozyme with the matrix material to prepare the film, and the stability of the egg white lysozyme is not improved, so it is difficult to exert long-term antibacterial properties. The film needs to be replaced frequently during use, and the film is easy to adhere to the wound, which has the risk of causing secondary damage to the wound. Summary of the invention

[0005] The purpose of the present invention is to provide a functional dressing based on egg white lysozyme and a preparation method thereof, which solves the following technical problems: (1) Egg white lysozyme has a poor effect on Gram-negative bacteria and cannot form a broad-spectrum antibacterial effect at the wound surface, resulting in an increased risk of wound infection; (2) Ordinary dressings only have a barrier effect, have a poor effect on promoting wound healing, and need to be replaced for a long time, which is easy to cause secondary damage to the wound.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A functional dressing based on egg white lysozyme comprises the following raw materials in parts by weight: 50-60 parts of hyaluronic acid, 3-5 parts of broad-spectrum lysozyme, 15-20 parts of multifunctional microcapsules, and 30-100 parts of deionized water; the broad-spectrum lysozyme is prepared by chemically bonding egg white lysozyme and perillaldehyde; the multifunctional microcapsules are prepared by using carboxymethyl cellulose and lactoferrin as shells and embedding squalene as cores.

[0008] Furthermore, the preparation method of the broad-spectrum lysozyme comprises the following steps:

[0009] S1: Mix potassium dihydrogen phosphate and sodium hydroxide solution, dilute with deionized water to make a phosphate buffer solution with a pH of 6.5-7;

[0010] S2: Place egg white lysozyme in a phosphate buffer solution, stir thoroughly, add perillaldehyde and a catalyst, react at room temperature for 5-6 hours, collect the product after centrifugation, and obtain a broad-spectrum lysozyme.

[0011] In this scheme, under the action of a catalyst, the amino group in the egg white lysozyme structure reacts with the aldehyde group in the perillaldehyde structure to produce a broad-spectrum lysozyme. This broad-spectrum lysozyme embeds perillaldehyde, which has an antibacterial effect on Gram-negative bacteria, into the molecular chain of egg white lysozyme by chemical bonding, which not only solves the problem that egg white lysozyme has a poor killing effect on Gram-negative bacteria, but also enables the prepared dressing to effectively prevent wound infection and reduce the possibility of inflammation. It can also improve the stability of egg white lysozyme, effectively prevent the loss of the enzyme, and enable the prepared dressing to exert an antibacterial effect for a long time, reduce the frequency of dressing replacement, avoid secondary damage to the wound, and help the healing of the wound.

[0012] Furthermore, in step S1, the concentration of the sodium hydroxide solution is 1-1.2 mol / L.

[0013] Furthermore, in step S2, the catalyst is any one of triethylamine and potassium carbonate.

[0014] Furthermore, the preparation method of the multifunctional microcapsule comprises the following steps:

[0015] SS1: Place nano calcium carbonate in deionized water and ultrasonically disperse for 10-12 minutes to form a calcium carbonate suspension;

[0016] SS2: Add lactoferrin to deionized water and stir thoroughly to form a lactoferrin solution;

[0017] SS3: Place carboxymethyl cellulose in deionized water, heat to 55-60°C and stir thoroughly to form a carboxymethyl cellulose solution;

[0018] SS4: Mix the calcium carbonate suspension and lactoferrin solution, disperse them by ultrasonic for 15-20 minutes, stir for 3-5 hours, add the carboxymethyl cellulose solution, and continue stirring for 5-6 hours to form a multi-component mixed solution;

[0019] SS5: centrifuging the multi-component mixed solution, washing with deionized water, adding a complexing agent, removing the calcium carbonate template, and centrifuging and washing to obtain microcapsules;

[0020] SS6: Squalene is placed in ethanol, mixed thoroughly, added to the prepared microcapsules, mixed and stirred at room temperature, and embedded. After completion, centrifugal washing is performed to obtain multifunctional microcapsules.

[0021] In this scheme, calcium carbonate is used as a template, lactoferrin and carboxymethyl cellulose are used to coat the surface of the calcium carbonate template through electrostatic action to form a shell layer of the microcapsule, and then the calcium carbonate template is removed under the action of a complexing agent to obtain a microcapsule with a cavity structure. The microcapsule is mixed with a squalene solution to produce an embedding effect, and squalene enters the cavity of the microcapsule to form a multifunctional microcapsule. The multifunctional microcapsule has lactoferrin and carboxymethyl cellulose as the shell layer. Lactoferrin has certain antibacterial activity, can reduce the risk of wound infection, and can bind to the receptors on the platelet membrane to activate the platelets to play a hemostatic role, effectively promoting wound healing. Carboxymethyl cellulose has good hygroscopicity. At the same time, the carboxyl group in its structure can also produce hydrogen bonds with the hydroxyl group in the base material hyaluronic acid structure to form a cross-linked network structure, enhance the dispersibility of the multifunctional microcapsule in the dressing, and the moisture absorption and breathability of the dressing, and provide a good repair environment for wound healing. Squalene can promote human metabolism, accelerate cell tissue repair, accelerate the process of wound healing, reduce the inflammatory response around the wound, relieve the symptoms of pain and redness, and promote healing and calm the wound. When it is used as the core layer of the multifunctional microcapsule, the protective effect of the multifunctional microcapsule can not only prolong the storage time of squalene, but also achieve the purpose of sustained release, so that squalene can act on the wound for a long time, significantly reducing the time of wound healing.

[0022] Furthermore, in step SS5, the complexing agent is ethylenediaminetetraacetic acid.

[0023] Furthermore, in step SS6, the mixing and stirring time at room temperature is 24-48 hours.

[0024] A method for preparing a functional dressing based on egg white lysozyme comprises the following steps:

[0025] (1) Place hyaluronic acid in deionized water, perform ultrasonic dispersion for 5-8 minutes, add broad-spectrum lysozyme, and stir at a speed of 150-200 r / min for 1-1.5 hours to obtain a mixture ①;

[0026] (2) placing the multifunctional microcapsules in deionized water and mixing them thoroughly to obtain a mixture ②;

[0027] (3) Add mixture ② into mixture ①, heat to 45-50℃ and stir thoroughly for 5-8h to form a functional dressing.

[0028] Beneficial effects of the present invention:

[0029] The present invention prepares broad-spectrum lysozyme and multifunctional microcapsules to participate in the preparation process of functional dressings, so that when the prepared functional dressing acts on the wound, the wound healing rate is as high as 48.7% on the 3rd day, the wound healing rate is as high as 92.3% on the 7th day, and the wound can be completely healed on the 12th day. The functional dressing has an excellent effect of promoting wound healing and can greatly reduce the healing time of the wounded. At the same time, the functional dressing also has excellent antibacterial performance, the antibacterial rate of Gram-positive bacteria Staphylococcus aureus is as high as 99.7%, and the antibacterial rate of Gram-negative bacteria Escherichia coli is as high as 99.1, and has a broad-spectrum antibacterial performance, which can greatly reduce the possibility of wound inflammation. The functional dressing prepared by the present invention can promote healing and antibacterial effects for a long time, and also has a calming and soothing effect on the wound. Even if it is used for a long time, it does not need to be replaced, which effectively avoids secondary damage to the wound caused by frequent dressing changes, and alleviates the pain of the wounded.

[0030] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0032] Figure 1It is the infrared spectrum of the broad-spectrum lysozyme in the embodiment of the present invention. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] The preparation methods of the broad-spectrum lysozyme and the multifunctional microcapsules in the following examples and comparative examples of the present invention are as follows:

[0035] 1. Preparation of broad-spectrum lysozyme

[0036] S1: Mix 0.68 g potassium dihydrogen phosphate with 29.1 ml of 1 mol / L sodium hydroxide solution, dilute to 100 ml with deionized water to prepare a phosphate buffer solution with a pH of 7;

[0037] S2: Place 3g of egg white lysozyme in 100ml of phosphate buffer solution, stir thoroughly, then add 2g of perillaldehyde and 0.02g of triethylamine, react at room temperature for 5h, collect the product after centrifugation, and obtain broad-spectrum lysozyme.

[0038] Broad-spectrum lysozyme was mixed with potassium bromide and ground into tablets, and then tested by infrared spectrum using Fourier transform infrared spectrometer. Figure 1 It can be seen that 3421cm -1 The absorption peak of nitrogen-hydrogen bond in imine group is 3046cm -1 The absorption peak of the carbon-hydrogen bond in the olefin group is 2934 cm -1 The absorption peak of the carbon-hydrogen bond in the methyl group is 1674 cm -1 The absorption peak of carbon-nitrogen double bond is at 3421cm -1 The nitrogen-hydrogen bond in the imine group and 1674 cm -1 The existence of the carbon-nitrogen double bond absorption peak at indicates that the amino group in the egg white lysozyme structure and the aldehyde group in the perillaldehyde structure undergo a Schiff base reaction.

[0039] 2. Preparation of multifunctional microcapsules

[0040] SS1: 1.8 g of nano calcium carbonate was placed in 30 ml of deionized water and ultrasonically dispersed for 10 min to form a calcium carbonate suspension;

[0041] SS2: Add 1 g lactoferrin into 50 ml deionized water and stir thoroughly to form a lactoferrin solution;

[0042] SS3: 1.5 g carboxymethyl cellulose was placed in 80 ml deionized water, heated to 55 °C and stirred thoroughly to form a carboxymethyl cellulose solution;

[0043] SS4: Mix the calcium carbonate suspension and lactoferrin solution, disperse them by ultrasonication for 15 min, stir for 3 h, add the carboxymethyl cellulose solution, and continue stirring for 5 h to form a multi-component mixed solution;

[0044] SS5: The multi-component mixture was centrifuged, washed with deionized water, 3 ml of ethylenediaminetetraacetic acid was added, the calcium carbonate template was removed, and microcapsules were obtained after centrifugation and washing;

[0045] SS6: 3 g of squalene was placed in 15 ml of ethanol, mixed thoroughly, added to the prepared microcapsules, mixed at room temperature for 24 h for embedding treatment, and then centrifuged and washed to obtain multifunctional microcapsules.

[0046] The supernatant was collected during centrifugal washing, and after the solvent was removed by rotary evaporation, the squalene in the supernatant was collected and weighed, and the embedding rate was calculated using the following formula:

[0047]

[0048] Wherein, Q is the embedding rate, %; W is the total mass of squalene, g; W1 is the mass of squalene in the supernatant, g; after calculation, the embedding rate of the multifunctional microcapsules is 43.2%.

[0049] Example 1, preparation method of functional dressing

[0050] (1) Place 25 g of hyaluronic acid in 60 ml of deionized water, disperse by ultrasonic for 5 min, add 1.5 g of broad-spectrum lysozyme, and stir at a speed of 150 r / min for 1 h to obtain a mixture ①;

[0051] (2) Place 7.5 g of multifunctional microcapsules in 50 ml of deionized water and mix thoroughly to obtain a mixture ②;

[0052] (3) Add mixture ② into mixture ①, heat to 45℃ and stir thoroughly for 5 hours to form a functional dressing.

[0053] Example 2, preparation method of functional dressing

[0054] (1) Place 28 g of hyaluronic acid in 65 ml of deionized water, disperse by ultrasonic for 7 min, add 1.8 g of broad-spectrum lysozyme, and stir at a speed of 180 r / min for 1.2 h to obtain a mixture ①;

[0055] (2) Place 8 g of multifunctional microcapsules in 60 ml of deionized water and mix thoroughly to obtain a mixture ②;

[0056] (3) Add mixture ② into mixture ①, heat to 47°C and stir thoroughly for 6.5 hours to form a functional dressing.

[0057] Example 3, preparation method of functional dressing

[0058] (1) Place 30 g of hyaluronic acid in 70 ml of deionized water, disperse by ultrasonication for 8 min, add 2.5 g of broad-spectrum lysozyme, and stir at a speed of 200 r / min for 1.5 h to obtain a mixture ①;

[0059] (2) Place 10 g of multifunctional microcapsules in 75 ml of deionized water and mix thoroughly to obtain a mixture ②;

[0060] (3) Add mixture ② into mixture ①, raise the temperature to 50℃ and stir thoroughly for 8 hours to form a functional dressing.

[0061] Comparative Example 1, Preparation Method of Functional Dressing

[0062] (1) 28 g of hyaluronic acid was placed in 65 ml of deionized water, and ultrasonically dispersed for 7 min. 1.8 g of broad-spectrum lysozyme was added, and stirred at a speed of 180 r / min for 1.2 h to obtain a mixture;

[0063] (2) Heat the mixture to 47°C and stir thoroughly for 6.5 hours to form a functional dressing.

[0064] Comparative Example 2, Preparation Method of Functional Dressing

[0065] (1) Place 28 g of hyaluronic acid in 65 ml of deionized water, disperse by ultrasonication for 7 min, and stir at a speed of 180 r / min for 1.2 h to obtain a mixture ①;

[0066] (2) Place 8 g of multifunctional microcapsules in 60 ml of deionized water and mix thoroughly to obtain a mixture ②;

[0067] (3) Add mixture ② into mixture ①, heat to 47°C and stir thoroughly for 6.5 hours to form a functional dressing.

[0068] Comparative Example 3, Preparation Method of Functional Dressing

[0069] (1) Place 28 g of hyaluronic acid in 65 ml of deionized water, disperse by ultrasonication for 7 min, add 1.8 g of egg white lysozyme, and stir at a speed of 180 r / min for 1.2 h to obtain a mixture ①;

[0070] (2) Place 8 g of multifunctional microcapsules in 60 ml of deionized water and mix thoroughly to obtain a mixture ②;

[0071] (3) Add mixture ② into mixture ①, heat to 47°C and stir thoroughly for 6.5 hours to form a functional dressing.

[0072] Comparative Example 4, Preparation Method of Functional Dressing

[0073] (1) Place 28 g of hyaluronic acid in 65 ml of deionized water, disperse by ultrasonic for 7 min, add 1.8 g of broad-spectrum lysozyme, and stir at a speed of 180 r / min for 1.2 h to obtain a mixture ①;

[0074] (2) Place 8 g of squalene in 60 ml of deionized water and mix thoroughly to obtain a mixture ②;

[0075] (3) Add mixture ② into mixture ①, heat to 47°C and stir thoroughly for 6.5 hours to form a functional dressing.

[0076] Performance Testing

[0077] I: Take 80 rats weighing 240-260g, cut the hair on the right side of the back spine of each rat, and use a scalpel to form a circular wound with a diameter of 1.2cm and a depth of 2mm under anesthesia. Divide each rat into a group of 10 rats, and divide them into eight groups. Cover the wound with the functional dressings prepared in Example 1-Example 3 and Comparative Example 1-Comparative Example 4, and observe the wound healing rates of the rat wounds on the 3rd day, the 7th day and the 12th day. When changing the dressing, measure the wound area, and calculate the wound healing rate by the following formula: Wound healing rate = (area before treatment - area after treatment) / area before treatment × 100%; the control group used physiological saline to clean the wound and then covered it with traditional cotton gauze dressing; the test results are as follows:

[0078]

[0079] It can be seen from the above table that the functional dressings prepared in Examples 1 to 3 all have excellent abilities to promote wound healing. In the functional dressing of Comparative Example 1, no multifunctional microcapsules are added, so the wound healing speed is slow. In the functional dressing prepared in Comparative Example 2, no broad-spectrum lysozyme is added, but multifunctional microcapsules are added, so the wound healing speed is fast, but not as good as the embodiments. In the functional dressing prepared in Comparative Example 3, multifunctional microcapsules and egg white lysozyme are added, and the wound healing speed is fast, which has a good effect of promoting wound healing. In the functional dressing prepared in Comparative Example 4, squalene is directly added, and it can be seen that the wound healing speed is fast in the early stage, but the wound healing speed is slow in the middle and late stages, because squalene is affected by the outside world and gradually loses its effect.

[0080] II: The functional dressings prepared in Examples 1 to 3 and Comparative Examples 1 to 4 were used as samples, and the concentration after activation was 10 -5 1.5 ml of bacterial solution of Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli with CFU / ml were added dropwise to the sterilized sample surface, cultured at 37°C for 24 hours, 30 μL of the cultured bacterial solution was transferred and evenly spread on the solid culture medium, and then cultured at 37°C for 48 hours. The number of colonies on the culture medium was counted, and a blank experiment was performed at the same time. The antibacterial rate was calculated using the following formula:

[0081]

[0082] In the formula, G is the number of colonies on the culture medium counted in the blank test; g is the number of colonies on the culture medium counted in the sample experiment; the specific test results are shown in the table below:

[0083]

[0084] As can be seen from the above table, the functional dressings prepared in Examples 1 to 3 have excellent antibacterial ability against both Gram-negative bacteria and Gram-positive bacteria. The functional dressings prepared in Comparative Examples 1 and 4 have added broad-spectrum lysozyme, so they have excellent antibacterial effects on both Gram-negative bacteria and Gram-positive bacteria. The functional dressing prepared in Comparative Example 2 does not have broad-spectrum lysozyme added, so the antibacterial performance against both bacteria is poor. The functional dressing prepared in Comparative Example 3 directly adds egg white lysozyme without modification, so the functional dressing has excellent antibacterial effect against Gram-positive bacteria and poor antibacterial effect against Gram-negative bacteria.

[0085] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0086] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the scope defined by the concept of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A functional dressing based on egg white lysozyme, characterized in that: The invention comprises the following raw materials in parts by weight: 50-60 parts of hyaluronic acid, 3-5 parts of broad-spectrum lysozyme, 15-20 parts of multifunctional microcapsules and 30-100 parts of deionized water; the broad-spectrum lysozyme is prepared by connecting egg white lysozyme and perillaldehyde through chemical bonding; the multifunctional microcapsules are prepared by using carboxymethyl cellulose and lactoferrin as shells and embedding squalene as cores.

2. The functional dressing based on egg white lysozyme according to claim 1, characterized in that: The preparation method of the broad-spectrum lysozyme comprises the following steps: S1: Mix potassium dihydrogen phosphate and sodium hydroxide solution, dilute with deionized water to make a phosphate buffer solution with a pH of 6.5-7; S2: Place egg white lysozyme in a phosphate buffer solution, stir thoroughly, add perillaldehyde and a catalyst, react at room temperature for 5-6 hours, collect the product after centrifugation, and obtain a broad-spectrum lysozyme.

3. The functional dressing based on egg white lysozyme according to claim 2, characterized in that: In step S1, the concentration of the sodium hydroxide solution is 1-1.2 mol / L.

4. The functional dressing based on egg white lysozyme according to claim 2, characterized in that: In step S2, the catalyst is any one of triethylamine and potassium carbonate.

5. The functional dressing based on egg white lysozyme according to claim 1, characterized in that: The preparation method of the multifunctional microcapsule comprises the following steps: SS1: Place nano calcium carbonate in deionized water and ultrasonically disperse for 10-12 minutes to form a calcium carbonate suspension; SS2: Add lactoferrin to deionized water and stir thoroughly to form a lactoferrin solution; SS3: Place carboxymethyl cellulose in deionized water, heat to 55-60°C and stir thoroughly to form a carboxymethyl cellulose solution; SS4: Mix the calcium carbonate suspension and lactoferrin solution, disperse them by ultrasonic for 15-20 minutes, stir for 3-5 hours, add the carboxymethyl cellulose solution, and continue stirring for 5-6 hours to form a multi-component mixed solution; SS5: centrifuging the multi-component mixed solution, washing with deionized water, adding a complexing agent, removing the calcium carbonate template, and centrifuging and washing to obtain microcapsules; SS6: Squalene is placed in ethanol, mixed thoroughly, added to the prepared microcapsules, mixed and stirred at room temperature, and embedded. After completion, centrifugal washing is performed to obtain multifunctional microcapsules.

6. The functional dressing based on egg white lysozyme according to claim 5, characterized in that: In step SS5, the complexing agent is ethylenediaminetetraacetic acid.

7. The functional dressing based on egg white lysozyme according to claim 5, characterized in that: In step SS6, the mixing and stirring time at room temperature is 24-48 hours.

8. The method for preparing a functional dressing based on egg white lysozyme according to claim 1, characterized in that: The following steps are involved: (1) Place hyaluronic acid in deionized water, perform ultrasonic dispersion for 5-8 minutes, add broad-spectrum lysozyme, and stir at a speed of 150-200 r / min for 1-1.5 hours to obtain a mixture ①; (2) placing the multifunctional microcapsules in deionized water and mixing them thoroughly to obtain a mixture ②; (3) Add mixture ② into mixture ①, heat to 45-50℃ and stir thoroughly for 5-8h to form a functional dressing.

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