Essence containing tri-factor tripeptide for anti-wrinkle and skin elasticity enhancement and preparation method thereof

Abstract

The present invention relates to the technical field of cosmetics, and in particular to an essence containing tripeptide for anti-wrinkle and skin elasticity increase and a preparation method thereof. The raw materials of the essence include acetyl hexapeptide-3, carnosine, palmitoyl pentapeptide-4, ectoine, bosine, ergothioneine, papaya extract, epilobium fleisch extract, phalaenopsis extract, polyol and water. The epilobium fleisch extract and phalaenopsis extract prepared by the specific process of the present invention have a good effect of increasing skin elasticity, and the essence prepared according to the raw materials of the present invention can effectively promote the skin elasticity effect and reduce the skin wrinkle function after 2 months of use, and also has low irritation.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an essence containing tripeptide and capable of resisting wrinkles and increasing skin elasticity, and a preparation method thereof. Background Art

[0002] Essence includes specific dosage forms such as essence liquid and essence milk, which are common types of cosmetics. They are often used on facial skin and contain precious functional ingredients such as plant extracts, ceramides, squalane, peptides, etc., which have anti-aging, anti-wrinkle, moisturizing, whitening or spot removal functions. Essence is usually used after skin care lotion and before lotion or cream. Because essence is rich in multiple functional ingredients and has a high concentration, it is usually more expensive than other cosmetics.

[0003] Although essences on the market have various functions, the functional strengths of the products vary; and there are relatively few products that can significantly reduce wrinkles and increase skin elasticity. Therefore, providing an essence that can significantly enhance the anti-wrinkle and increase skin elasticity effects is a technical problem that needs to be solved at present. Summary of the invention

[0004] The present invention aims at the above technical problems and proposes a technical solution of the present invention, which includes an essence containing tripeptide and tri-factor for anti-wrinkle and increasing skin elasticity and a preparation method thereof.

[0005] The present invention comprises an essence containing tripeptide for anti-wrinkle and skin elasticity enhancement, wherein the raw materials include acetyl hexapeptide-3, carnosine, palmitoyl pentapeptide-4, ectoine, bosine, ergothioneine, papaya extract, willowherb extract, phalaenopsis extract, polyol and water.

[0006] Acetyl hexapeptide-3 is a synthetic acetylated and amidated hexapeptide composed of 12-17 amino acid residues of synaptosome-associated protein 25 (SNAP-25). It inhibits the formation of SNARE complexes in vitro in a concentration-dependent manner and inhibits calcium-induced norepinephrine release in permeabilized chromaffin cells. When topically administered, this ingredient can inhibit the decrease in type I collagen levels and skin thickness in the skin in the D-galactose-induced subacute aging mouse model, as well as the increase in type III collagen levels, thereby increasing skin collagen and effectively combating skin aging.

[0007] Carnosine can remove free radicals and reduce the damage of oxidative stress to the skin, thereby delaying the aging process of the skin. Carnosine can increase the skin's ability to retain moisture, making the skin look more moisturized and shiny, while also helping to brighten the skin tone and make it fairer. Carnosine can react with active substances in the body to protect proteins from being glycosylated, thereby reducing the phenomenon of skin aging. Carnosine can enhance the skin's ability to repair itself and increase the skin's resistance to harmful external factors.

[0008] Palmitoyl pentapeptide-4 is a chemical substance that can effectively improve skin roughness, reduce the number of fine lines, reduce the depth and area of ​​wrinkles, etc. As a signal peptide, it can penetrate the dermis to increase collagen and reverse skin aging by rebuilding from the inside out. In addition, palmitoyl pentapeptide-4 can also stimulate the synthesis of key components of the skin matrix, such as collagen, etc. As a messenger peptide, it stimulates the synthesis and development of collagen (type I, III, IV) and elastin in the dermis of the skin, thereby improving skin quality. It can promote the synthesis of macromolecules such as collagen, fibronectin and glycosaminoglycans by acting on dermal fibroblasts, repair and rebuild the extracellular matrix and the epidermal-dermal junction (DEJ), solve wrinkle problems from deep in the skin and improve skin appearance. At the same time, it can also promote the expression of wound repair genes, which is beneficial to the recovery of skin tissue. In addition, palmitoyl pentapeptide-4 is also mild and non-irritating. It is an effective moisturizer and antioxidant that can increase the water content of the skin, thereby playing a moisturizing and anti-oxidant role, and has a significant effect on improving skin condition.

[0009] The sunscreen effect of Ecdoin in cosmetics is achieved by absorbing ultraviolet (UV) radiation. Ecdoin can absorb UV-A and UV-B radiation, and its maximum absorption wavelengths are 340nm and 287nm respectively. When ultraviolet rays are irradiated to the surface of the skin, Ecdoin absorbs UV radiation, thereby protecting the skin from ultraviolet damage. Compared with other sunscreens, Ecdoin has a wider absorption range and better photostability. It can effectively resist ultraviolet radiation, thereby protecting the skin from sun damage. Ecdoin also has a certain skin protection effect. Studies have shown that Ecdoin can promote blood circulation in the skin, enhance the skin's metabolism and immune function, thereby protecting the skin's health. In addition, Ecdoin also has a certain antioxidant effect, which can scavenge free radicals and reduce oxidative stress, thereby protecting the skin from damage caused by environmental pollution.

[0010] Bosin is a mixture of glycoproteins derived from xylose, which has anti-aging activity. It can promote the skin's absorption of active ingredients in cosmetics, enhance the skin's barrier function, improve the skin's ability to retain moisture, reduce wrinkles and fine lines, and lift and tighten the skin. Due to its significant anti-aging effect and safety, Bosin is widely used in various anti-aging cosmetics. Bosin can replenish the skin with the required moisture, prevent moisture loss, and improve skin dryness and dullness; it contains activated mucopolysaccharide compounds, promotes collagen regeneration, increases skin elasticity, and delays skin aging; it can simulate the effect of epidermal growth factor, stimulate collagen synthesis by regulating extracellular matrix components, and improve skin elasticity and firmness; it can repair damaged stratum corneum, restore skin integrity, enhance skin barrier function, improve skin water retention, and reduce sensitivity to external environmental stimuli; it can regulate the signaling pathways in keratinocytes, increase skin moisture content and reduce its loss rate; it can promote the production of hyaluronic acid and elastic fibers, and has a diluting effect on static and dynamic lines; it can promote fibroblast proliferation and secretion of collagen and elastin, and improve skin firmness.

[0011] Ergothioneine is a natural antioxidant that can neutralize free radicals and protect the skin from oxidative damage. Its antioxidant capacity is comparable to or even stronger than conventional antioxidants such as glutathione and ascorbic acid. Ergothioneine can remove hydroxyl radicals, peroxynitrates and nitrogen radicals in the skin and protect skin cells from damage. Ergothioneine can inhibit inflammatory responses and reduce damage to normal cells in the body. It reduces skin inflammation by inhibiting the activation of NF-kB and reducing the production of inflammatory factors. It can scavenge free radicals while moisturizing, prevent skin dryness and keep the skin hydrated. Ergothioneine can inhibit the activity of tyrosinase and reduce the production of melanin, thereby helping to lighten spots and brighten the skin. Ergothioneine can remove free radicals that cause skin aging, reduce damage to collagen and elastic fibers, and improve problems such as skin sagging and wrinkles.

[0012] Preferably, the mass ratio of Epilobium fleischerii extract to Phalaenopsis orchid extract is 1:1.2-2.

[0013] Preferably, the essence comprises the following raw materials in percentage by mass: 0.005-0.5% acetyl hexapeptide-3, 0.005-0.3% carnosine, 0.002-0.2% palmitoyl pentapeptide-4, 0.004-0.3% ectoine, 0.005-0.4% bosine, 0.005-0.6% ergothioneine, 0.08-1.2% papaya extract, 0.04-0.9% epilobium fleischer extract, 0.05-1.3% phalaenopsis extract, 5-15% polyol and the balance water.

[0014] Preferably, in the essence, the polyol includes at least one of ethylene glycol, propylene glycol, glycerol, butylene glycol, pentanediol and sorbitol.

[0015] Preferably, in the essence, the preparation method of the Epilobium fleischerii extract comprises the following steps:

[0016] Step 1: crush and sieve the Epilobium fleischii to obtain powder, add water, heat and stir to obtain a material;

[0017] Step 2: Add ethanol to the material, heat and stir, filter, and obtain a filtrate;

[0018] Step 3: ultrafiltration of the filtrate, concentration, drying and sterilization of the ultrafiltrate to obtain the Epilobium Freiherii extract.

[0019] Preferably, in step 1, the mass of water added is 5-30 times the mass of the powder; the temperature of heating and stirring is 50-75° C., and the time is 1-5 hours.

[0020] Preferably, in step 2, the amount of ethanol added is 0.25-0.65 times the mass of water added in step 1, the heating and stirring temperature is 45-55° C., and the time is 2-7 hours.

[0021] Preferably, in step 3, ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cutoff of 1000 Da.

[0022] Preferably, the preparation method of the Phalaenopsis extract comprises the following steps:

[0023] Step A: crushing Phalaenopsis, adding water and cellulase, heating and stirring, and then cooling to obtain a cooled material;

[0024] Step B: Cooling the material to inactivate enzymes and sterilize, returning to room temperature to obtain a treated material, then adding glucose and fermentation bacteria to the treated material to obtain an intermediate material, and fermenting to obtain a fermented material;

[0025] Step C: coarse filtering the fermented material, ultrafiltering the coarse filtrate to obtain an ultrafiltrate, freeze-drying the ultrafiltrate, and sterilizing it to obtain the Phalaenopsis extract.

[0026] Preferably, in step A, the mass of water and cellulase added is 10-30 times and 0.1-0.3 times of the mass of crushed Phalaenopsis, respectively, and the heating and stirring temperature is 40-60° C. for 3-8 hours.

[0027] Preferably, in step B, the mass of glucose is 2-6% of the mass of the intermediate material.

[0028] Preferably, in step C, ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cutoff of 1200 Da.

[0029] Preferably, the fermentation bacteria are composed of Lactobacillus paracasei CICC 6237 and Bacillus subtilis CICC 24713 in a quantitative ratio of 1:0.3-0.8.

[0030] Preferably, the total amount of fermentation bacteria in the intermediate material is 3.5×10 8 -9×10 8 cfu / g.

[0031] Preferably, the fermentation temperature is 29-39° C. and the fermentation time is 60-90 hours.

[0032] The present invention also provides a composition, which comprises the above-mentioned Epilobium fleischii extract and Phalaenopsis extract, and the mass ratio of the Epilobium fleischii extract to the Phalaenopsis extract is 1:1.2-2.

[0033] The solution of the present invention also provides a method for preparing the essence, comprising putting water into a container, adding polyol, stirring evenly, then adding the remaining raw materials, stirring evenly, sterilizing, and obtaining the essence.

[0034] Beneficial effects of the present invention:

[0035] The present invention uses acetyl hexapeptide-3, carnosine, palmitoyl pentapeptide-4, ectoine, bosine, ergothioneine, papaya extract, epilobium fleisch extract, phalaenopsis extract, polyol and water to prepare essence. The epilobium fleisch extract and phalaenopsis extract prepared by the specific process of the present invention have a good effect of increasing skin elasticity, and the prepared essence can effectively promote the skin elasticity effect and reduce skin wrinkle function after being used for 2 months, and also has low irritation.

[0036] The epilobium fleischerii extract and the phalaenopsis orchid extract prepared by the invention have good skin elasticity increasing effect at low concentrations, and give the skin good elasticity.

[0037] The specific fermentation process of the present invention can significantly improve the effect of Phalaenopsis extract in increasing skin elasticity, and the selection of the specific fermentation bacteria of the present invention can improve the effect of the extract in increasing skin elasticity compared with other bacteria for fermentation; the present invention uses Lactobacillus paracasei CICC 6237 and Bacillus subtilis CICC 24713 for matching, which can enrich the types and concentrations of active factors, and mutually promote the effect of promoting skin elasticity enhancement, and the two have a synergistic effect on the effect. In particular, when the extract is fermented with Lactobacillus paracasei CICC 6237 and Bacillus subtilis CICC 24713 fermentation bacteria at a ratio of 1:0.3-0.8, the obtained extract has a better effect.

[0038] The essence prepared by the invention has excellent hypoallergenicity and will not produce irritation effect on the skin.

[0039] The essence prepared by the present invention has a good function of promoting skin elasticity. The degree of skin elasticity R2 value increased by 937×10 -4 , has a good experience effect. The combination of Bossain and papaya extract can also effectively enhance the effect of increasing skin elasticity. DETAILED DESCRIPTION

[0040] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0041] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0042] Lactobacillus paracasei CICC 6237: number CICC 6237, purchased from China Food Fermentation Industry Research Institute Co., Ltd. Bacillus subtilis CI CC 24713: number CI CC 24713, purchased from China Food Fermentation Industry Research Institute Co., Ltd. Bacillus plantarum CI CC 24998: number CI CC 24998, purchased from China Food Fermentation Industry Research Institute Co., Ltd. Papaya extract, purchased from Baoji Cosmeco Cosmetics Raw Materials Development Co., Ltd.

[0043] 1. Preparation and performance test of Epilobium fleischii extract and Phalaenopsis extract Epilobium fleischii extract 1: The preparation method thereof is as follows:

[0044] Step 1: Grind and sieve the roots of Epilobium fleischerii to obtain a powder with a particle size of 0.01-0.2 mm, add water 25 times the mass of the powder, heat to 60° C. and stir at a speed of 70 rpm for 1.5 hours to obtain a material;

[0045] Step 2: Add 0.4 times the mass of ethanol as compared to the water added in step 1 to the material, heat to 50°C, stir at a speed of 70 rpm for 5.8 hours, and filter using a filter membrane with a pore size of 0.5 microns to obtain a filtrate;

[0046] Step 3: The filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 1000 Da, the ultrafiltrate is concentrated to a mass of 20.5% of the ultrafiltrate mass, and freeze-dried to a water content of 3.26% to obtain the Epilobium fleischerii extract.

[0047] Epilobium fleischerii extract 2: The preparation method thereof is as follows:

[0048] Step 1: Grind and sieve the roots of Epilobium fleischerii to obtain a powder with a particle size of 0.01-0.2 mm, add water 20 times the mass of the powder, heat to 60° C. and stir at a speed of 70 rpm for 1.8 hours to obtain a material;

[0049] Step 2: Add 0.25 times the mass of ethanol as much as the water added in step 1 to the material, heat to 48° C., stir at a speed of 70 rpm for 6.2 hours, and filter using a filter membrane with a pore size of 0.5 μm to obtain a filtrate;

[0050] Step 3: The filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 1000 Da, the ultrafiltrate is concentrated to a mass of 18.7% of the ultrafiltrate mass, and freeze-dried to a water content of 3.19% to obtain the Epilobium fleischerii extract.

[0051] Epilobium fleischii extract 3: The preparation method thereof is as follows:

[0052] Step 1: Grind and sieve the roots of Epilobium fleischerii to obtain a powder with a particle size of 0.01-0.2 mm, add water 18 times the mass of the powder, heat to 60° C. and stir at a speed of 70 rpm for 2 hours to obtain a material;

[0053] Step 2: Add 0.65 times the mass of ethanol as compared to the water added in step 1 to the material, heat to 55° C., stir at a speed of 70 rpm for 7 hours, and filter using a filter membrane with a pore size of 0.5 μm to obtain a filtrate;

[0054] Step 3: The filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 1000 Da, the ultrafiltrate is concentrated to a mass of 19.7% of the ultrafiltrate mass, and freeze-dried to a water content of 3.31% to obtain the Epilobium fleischerii extract.

[0055] Phalaenopsis extract 1: The preparation method thereof is as follows:

[0056] Step A: Crush the leaves of Phalaenopsis to a particle size of less than 0.5 mm, add 20 times the mass of water and 0.25 times the mass of cellulase (enzyme activity 50,000 U / g) respectively, heat to 55° C., stir at 70 rpm for 3.5 hours, and then cool to room temperature to obtain a cooled material;

[0057] Step B: Cooling the material to inactivate enzymes and sterilize, returning to room temperature to obtain a treated material, then adding glucose and fermentation bacteria to the treated material to obtain an intermediate material, and aerobically fermenting at a temperature of 37.5°C for 82 hours to obtain a fermented material; wherein glucose accounts for 4.92% of the mass of the intermediate material;

[0058] Step C: The fermented material is coarsely filtered using a filter membrane with a pore size of 1 micron, and the coarse filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 1200 Da to obtain an ultrafiltrate, and the ultrafiltrate is freeze-dried to a water content of 3.76% to obtain the Phalaenopsis extract.

[0059] The fermentation bacteria consisted of Lactobacillus paracasei CI CC 6237 and Bacillus subtilis CI CC 24713 in a ratio of 1:0.5; the total amount of fermentation bacteria in the intermediate material was 6.60×10 8 cfu / g.

[0060] Phalaenopsis extract 2: The preparation method thereof is as follows:

[0061] Step A: Crush the leaves of Phalaenopsis to a particle size of less than 0.5 mm, add 22 times the mass of water and 0.28 times the mass of cellulase (enzyme activity 50,000 U / g) respectively, heat to 52° C., stir at 70 rpm for 3.2 hours, and then cool to room temperature to obtain a cooled material;

[0062] Step B: Cooling the material to inactivate enzymes and sterilize, returning to room temperature to obtain a treated material, then adding glucose and fermentation bacteria to the treated material to obtain an intermediate material, and aerobically fermenting at a temperature of 38° C. for 80 hours to obtain a fermented material; wherein glucose accounts for 5.04% of the mass of the intermediate material;

[0063] Step C: The fermented material is coarsely filtered using a filter membrane with a pore size of 1 micron, and the coarse filtrate is ultrafiltered using an ultrafiltration membrane with a molecular weight cutoff of 1200 Da to obtain an ultrafiltrate, and the ultrafiltrate is freeze-dried to a water content of 3.68% to obtain the Phalaenopsis extract.

[0064] The fermentation bacteria consisted of Lactobacillus paracasei CI CC 6237 and Bacillus subtilis CI CC 24713 in a ratio of 1:0.8; the total amount of fermentation bacteria in the intermediate material was 6.72×10 8 cfu / g.

[0065] Phalaenopsis extract 3: The preparation method thereof is as follows:

[0066] Step A: Crush the leaves of Phalaenopsis to a particle size of less than 0.5 mm, add 20 times the mass of water and 0.25 times the mass of cellulase (enzyme activity 50,000 U / g) respectively, heat to 55° C., stir at 70 rpm for 3.5 hours, and then cool to room temperature to obtain a cooled material;

[0067] Step B: Cooling the material to inactivate enzymes and sterilize, returning to room temperature to obtain a treated material, then adding glucose to the treated material to obtain an intermediate material, placing it at a temperature of 37.5° C. for 82 hours to obtain a placed material; wherein glucose accounts for 4.92% of the mass of the intermediate material;

[0068] Step C: placing the material and using a filter membrane with a pore size of 1 micron to perform coarse filtration, using an ultrafiltration membrane with a molecular weight cutoff of 1200Da to perform ultrafiltration to obtain an ultrafiltrate, and freeze-drying the ultrafiltrate to a water content of 3.76% to obtain the Phalaenopsis extract.

[0069] Phalaenopsis extract 4: The preparation method thereof is as follows:

[0070] The only difference from Phalaenopsis extract 1 is that the fermentation bacteria is Lactobacillus casei CICC 6237, and the total amount of fermentation bacteria in the intermediate material is 6.60×10 8 cfu / g; others are the same as Phalaenopsis Extract 1.

[0071] Phalaenopsis extract 5: The preparation method thereof is as follows:

[0072] The only difference from Phalaenopsis Extract 1 is that the fermentation bacteria is Bacillus subtilis CICC 24713, and the total amount of fermentation bacteria in the intermediate material is 6.60×10 8 cfu / g; others are the same as Phalaenopsis Extract 1.

[0073] Phalaenopsis extract 6: The preparation method thereof is as follows:

[0074] The only difference from Phalaenopsis extract 1 is that the fermentation bacteria are composed of Lactobacillus paracasei CICC6237 and Bacillus subtilis CICC 24713 in a ratio of 1:0.1; the total amount of fermentation bacteria in the intermediate material is 6.60×10 8 cfu / g; others are the same as Phalaenopsis Extract 1.

[0075] Phalaenopsis extract 7: Its preparation method is as follows:

[0076] The only difference from Phalaenopsis extract 1 is that the fermentation bacteria are composed of Lactobacillus paracasei CICC6237 and Bacillus subtilis CICC 24713 in a ratio of 1:1.5; the total amount of fermentation bacteria in the intermediate material is 6.60×10 8 cfu / g; others are the same as Phalaenopsis Extract 1.

[0077] Phalaenopsis extract 8: The preparation method thereof is as follows:

[0078] The only difference from Phalaenopsis extract 1 is that the fermentation bacteria is Lactobacillus plantarum CI CC 24998; the total amount of fermentation bacteria in the intermediate material is 6.60×10 8 cfu / g; others are the same as Phalaenopsis Extract 1.

[0079] The above extracts (and the combination of the two types of extracts) were diluted to a mass concentration of 1.5% (the diluent was a 10wt% glycerol aqueous solution) to obtain samples, and then the samples were tested for their effect of increasing skin elasticity. The specific method is: female volunteers aged 35-45 with healthy skin were selected, and randomly divided into groups of 7 people each. After washing their faces every night, each group of volunteers used about 0.8g of the above sample to evenly apply it on the face for 2 consecutive months. The test tester MPA580 tested the skin elasticity value R2 (the ratio of the rebound amount of the skin without negative pressure to the maximum stretching amount when there is negative pressure, the closer to 1, the better the elasticity, and the theoretical range of this parameter is between 0-1) and calculated the R2 change value △R2 after using the sample for 2 months compared to before using the sample. The results of the effect of increasing skin elasticity of each extract and the combination of extracts are shown in Tables 1 and 2.

[0080] Table 1: Effects of various extracts on increasing skin elasticity

[0081]

[0082]

[0083] Table 2: Composition of extracts to increase skin elasticity

[0084] Sample / total concentration is 1.5wt% <![CDATA[△R2 / ×10 -4 ]]> Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:0.2 554 Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:0.8 560 Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:1.2 573 Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:1.5 581 Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:2 574 Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:2.5 559 Fleisher's Epilobium Extract 1 + Phalaenopsis Extract 1 Ratio 1:3 553

[0085] It can be seen from the test results in Table 1-2 that the Epilobium fleischerii extract and Phalaenopsis orchid extract prepared by the present invention have a good effect of increasing skin elasticity at low concentrations, and impart better elasticity to the skin.

[0086] The test comparison of Phalaenopsis extracts 1-2 and 3-8 in Table 1 shows that the specific fermentation process of the present invention can significantly improve the effect of Phalaenopsis extract in increasing skin elasticity, and the selection of specific fermentation bacteria of the present invention can improve the effect of extract in increasing skin elasticity compared with other bacteria for fermentation; the present invention uses Lactobacillus paracasei CI CC 6237 and Bacillus subtilis CI CC 24713 for matching, which can enrich the types and concentrations of active factors, and promote the effect of promoting skin elasticity enhancement by mutual promotion, and the two have a synergistic effect on this effect. In particular, when the extract is fermented with Lactobacillus paracasei CI CC 6237 and Bacillus subtilis CI CC 24713 fermentation bacteria at a ratio of 1:0.3-0.8, the obtained extract has a better effect.

[0087] 2. Preparation and performance testing of essence

[0088] The essence is prepared according to the contents of Table 3.

[0089] Table 3: Raw material composition of essence

[0090]

[0091]

[0092] The preparation method of the essence is as follows: put water into a container, add polyol, stir at 65 rpm for 0.3 hours, then add the remaining raw materials, continue stirring for 0.4 hours, sterilize, and obtain the essence.

[0093] The above essence was subjected to the following performance tests.

[0094] 1. Human skin patch test

[0095] Volunteers with sensitive skin aged 30-50 were selected and randomly divided into 5 groups, 10 in each group. The skin in the test area was washed with clean water and dried naturally. Then, a patch tester was selected and 0.02g of the above essence was taken into the patch tester by closed patch test. The patch was taped on the back of the volunteer. The volunteer kept the patch dry for 48 hours. After 48 hours, the tester was removed. After waiting for 30 minutes and the indentation disappeared, the visual inspection was performed. The visual inspection results were recorded according to the skin reaction grade standard recorded in Table 4. The results are shown in Table 5.

[0096] Table 4: Skin reaction grade standard

[0097]

[0098]

[0099] Table 5: Human skin patch test

[0100]

[0101] The above tests show that the essence prepared by the present invention has excellent hypoallergenicity and will not cause irritation to the skin.

[0102] 2. Test on the effect of increasing skin elasticity

[0103] Female volunteers aged 35-45 with healthy skin were selected, and randomly divided into groups with 7 people in each group. After washing their faces every night, volunteers in each group evenly applied about 0.8g of the above sample on their faces for 2 consecutive months. The test instrument MPA580 tested the skin elasticity value R2 (the ratio of the rebound amount of skin without negative pressure to the maximum stretching amount under negative pressure, the closer to 1, the better the elasticity, and the theoretical range of this parameter is between 0-1) and calculated the R2 change value △R2 after using the sample for 2 months compared with before using the sample. The results of the effect of each essence on increasing skin elasticity are shown in Table 6.

[0104] Table 6: Effect of essence on increasing skin elasticity

[0105] test <![CDATA[△R2 / ×10 -4 ]]> Essence 1 937 Essence 2 928 Essence 3 802 Essence 4 920 Essence 5 913

[0106] According to the test results in Table 6, the essence prepared by the present invention has a good function of promoting skin elasticity. The degree of skin elasticity R2 value increased by 937×10 -4 , with a better experience effect. The test results of Essence 3-5 and Essence 1 show that Bossain and papaya extract can effectively enhance the effect of increasing skin elasticity.

[0107] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An essence containing tripeptide for anti-wrinkle and skin elasticity enhancement, characterized in that: According to the mass percentage, the raw materials include: 0.005-0.5% acetyl hexapeptide-3, 0.005-0.3% carnosine, 0.002-0.2% palmitoyl pentapeptide-4, 0.004-0.3% ectoine, 0.005-0.4% bosaicin, 0.005-0.6% ergothioneine, 0.08-1.2% papaya extract, 0.04-0.9% Fleisher's epilobium extract, 0.05-1.3% Phalaenopsis extract, 5-15% polyol and the balance water; The mass ratio of epilobium fleischii extract to phalaenopsis extract is 1:1.2-2; The preparation method of the Epilobium freiherb extract comprises the following steps: Step 1: crush and sieve the Epilobium fleischii to obtain powder, add water, heat and stir to obtain a material; Step 2: Add ethanol to the material, heat and stir, filter, and obtain a filtrate; Step 3: ultrafiltration of the filtrate, concentration, drying and sterilization of the ultrafiltrate to obtain the Epilobium Freiherii extract; In step 1, the mass of water added is 5-30 times the mass of the powder; the heating and stirring temperature is 50-75°C and the time is 1-5 hours; In step 2, the amount of ethanol added is 0.25-0.65 times the mass of water added in step 1, and the heating and stirring temperature is 45-55° C. and the time is 2-7 hours; In step 3, ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cutoff of 1000 Da; The preparation method of the Phalaenopsis extract comprises the following steps: Step A: crushing Phalaenopsis, adding water and cellulase, heating and stirring, and then cooling to obtain a cooled material; Step B: Cooling the material to inactivate enzymes and sterilize, returning to room temperature to obtain a treated material, then adding glucose and fermentation bacteria to the treated material to obtain an intermediate material, and fermenting to obtain a fermented material; Step C: coarse filtering the fermented material, ultrafiltering the coarse filtrate to obtain an ultrafiltrate, freeze-drying the ultrafiltrate, and sterilizing it to obtain the Phalaenopsis extract; In step A, the mass of water and cellulase added is 10-30 times and 0.1-0.3 times of the mass of crushed Phalaenopsis, respectively, and the heating and stirring temperature is 40-60° C. for 3-8 hours; In step B, the mass of glucose is 2-6% of the mass of the intermediate material; In step C, ultrafiltration is performed using an ultrafiltration membrane with a molecular weight cutoff of 1200 Da; The fermentation bacteria consisted of Lactobacillus paracasei CICC 6237 and Bacillus subtilis CICC24713 in a ratio of 1:0.3-0.8, and the total amount of fermentation bacteria in the intermediate material was 3.5×10 8 -9×10 8 cfu / g, the fermentation temperature is 29-39℃ and the time is 60-90 hours.

2. The essence according to claim 1, characterized in that The polyol includes at least one of ethylene glycol, propylene glycol, glycerol, butylene glycol, pentanediol and sorbitol.

3. The method for preparing the essence according to any one of claims 1-2, characterized in that: The process comprises the steps of putting water into a container, adding polyol, stirring evenly, then adding the remaining raw materials, stirring evenly, sterilizing, and obtaining the essence.

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