A nitrilase immobilization technology production equipment for nicotinic acid production

Abstract

The present invention discloses a nitrilase immobilization technology production equipment for nicotinic acid production, comprising an immobilization box, an immobilization mechanism arranged on the inner wall of the immobilization box, a liquid inlet pipe, the liquid inlet pipe is used to input nitrilase liquid into the immobilization box, a crosslinking agent input pipe, the crosslinking agent input pipe is used to input the crosslinking agent into the immobilization box, a processing mechanism, the processing mechanism is used to purify and discharge the residual liquid, and a storage mechanism. The present invention relates to the technical field of nitrilase immobilization equipment; after the nitrilase enters the immobilization box, the nitrilase can be immobilized and cleaned through the cooperation of a first driving motor, a transmission shaft, a stirring blade, a friction disk, a friction ring plate, a through hole, a heat-insulating ring plate, an electric push rod and a sliding ring, so as to avoid impurities adhering to the solid surface of the nitrilase to affect its storage, and at the same time, the soft pad is arranged to avoid damage to the nitrilase solid when the stirring blade rotates at a high speed.

Description

Technical Field

[0001] The invention relates to the technical field of nitrilase immobilization equipment, in particular to nitrilase immobilization technology production equipment for nicotinic acid production. Background Art

[0002] Nitrilase is an enzyme having a nitrile hydration activity that hydrates a nitrile group and converts it into an amide group. The amide compound corresponding to the nitrile compound is produced using the enzyme or a microbial cell containing the enzyme, and it is known that the conversion rate and selectivity of the nitrile compound to the corresponding amide compound are higher than those of the existing chemical synthesis method.

[0003] The existing nitrilase immobilization technology production equipment for nicotinic acid production has a relatively simple structure. Currently, when the nitrilase needs to be washed after immobilization, if it is quickly stirred, the nitrilase fixation will be damaged by impact. At the same time, the excess cross-linking agent and nitrilase liquid mixture generated after the nitrilase immobilization is completed will be directly discharged, causing environmental pollution.

[0004] To this end, the present invention provides a nicotinic acid production equipment using nitrilase immobilization technology to solve the above problems. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention provides a nicotinic acid production equipment using nitrilase immobilization technology to solve the above problems.

[0006] To achieve the above objectives, the present invention is implemented by the following technical scheme: a nicotinic acid production nitrilase immobilization technology production equipment, comprising:

[0007] A fixation box, and a fixation mechanism arranged on the inner wall of the fixation box;

[0008] A liquid inlet pipe, which is used to input nitrilase liquid into the immobilization box;

[0009] A cross-linking agent input tube, which is used to input the cross-linking agent into the immobilization box;

[0010] A processing mechanism, the processing mechanism is used to purify and discharge the residual liquid;

[0011] A storage mechanism, the storage mechanism is used to fix the nitrilase for frozen storage;

[0012] A washing pipe, which is used to input detergent into the interior of the fixed box;

[0013] The bottom of the immobilization box is fixedly connected to the top of the processing mechanism, the inner wall of the immobilization box is rotatably connected to the side wall of the immobilization mechanism, the top of the immobilization box is connected to the bottom of the liquid inlet pipe, the top of the immobilization box is connected to the bottom of the cross-linking agent input pipe, the top of the immobilization box is connected to the bottom of the washing pipe, and the inner wall of the processing mechanism is fixedly connected to the side wall;

[0014] The immobilization mechanism comprises a first drive motor, the bottom of the first drive motor is fixedly connected to the top of the immobilization box, the output end of the first drive motor is fixedly connected to a transmission shaft, the side wall of the transmission shaft is fixedly connected to a stirring blade, one side of the stirring blade is fixedly connected to a friction disk, the inner wall of the immobilization box is fixedly connected to a friction ring plate, a through hole is opened inside the friction ring plate, the inner wall of the immobilization box is fixedly connected to a heat preservation ring plate, the inner wall of the heat preservation ring plate is fixedly connected to an electric push rod, the bottom of the electric push rod is fixedly connected to a sliding ring, the bottom of the immobilization box is connected to a drain pipe, and the bottom of the immobilization box is connected to a discharge pipe;

[0015] The treatment mechanism comprises a treatment box, the bottom of the fixed box body is fixedly connected to the top of the treatment box, the side wall of the treatment box is fixedly connected to an electric slide rail, the electric slide rail is slidably connected to a sealing slide plate through an electronic slider, the inner wall of the treatment box is fixedly connected to a purification filter screen, and one side of the treatment box is connected to a drain pipe;

[0016] The storage mechanism includes a conveyor belt, the side wall of the conveyor belt is fixedly connected to the inner wall of the processing box, the inner wall of the conveyor belt is rotatably connected to a rotating round rod, one side of the rotating round rod is fixedly connected to a second driving motor, the top of the conveyor belt is fixedly connected to a clamping cylinder, the top of the inner wall of the clamping cylinder is fixedly connected to a first trigger switch, the top of the inner wall of the clamping cylinder is fixedly connected to a first limit spring, the top of the first limit spring is fixedly connected to a supporting circular plate, and the top of the supporting circular plate is movably connected to a refrigeration cylinder.

[0017] Preferably: the immobilization mechanism also includes a soft pad, the inner wall of the soft pad is fixedly connected to the side wall of the stirring blade, one side of the friction disk is slidably connected to the inner wall of the friction ring plate, a filter is provided inside the through hole, the side wall of the sliding ring is slidably connected to the inner wall of the insulation ring plate, the bottom of the first drive motor is fixedly connected to the top of the immobilization box, and the transmission shaft passes through the immobilization box.

[0018] Preferably: the processing mechanism also includes a garbage storage tank, the side wall of the sealing slide is fixedly connected to one side of the garbage storage tank, one side of the inner wall of the processing box is fixedly connected to an electric telescopic rod, one side of the electric telescopic rod is fixedly connected to a cleaning brush, one side of the cleaning brush is fixedly connected to a telescopic plate, the bottom of the inner wall of the processing box is fixedly connected to a humidity sensor, and the top of the cleaning brush is fixedly connected to an inclined plate.

[0019] Preferably: one side of the inclined plate is fixedly connected to one side of the inner wall of the processing box, one side of the telescopic plate is fixedly connected to one side of the inner wall of the processing box, the bottom of the cleaning brush is slidably connected to the top of the purification filter, and one side of the sealing slide plate is slidably connected to the side wall of the processing box.

[0020] Preferably: the storage mechanism also includes a second trigger switch, the bottom of the inner wall of the refrigeration cylinder is fixedly connected to the bottom of the second trigger switch, the bottom of the inner wall of the refrigeration cylinder is fixedly connected to a second limit spring, the top of the second limit spring is fixedly connected to a sliding disk, the top of the sliding disk is fixedly connected to an electric rotating rod, the top of the electric rotating rod is fixedly connected to a rotating disk, the top of the rotating disk is fixedly connected to a fixed plate, and the second trigger switch is provided with a solenoid valve.

[0021] Preferably, one side of the fixing plate is slidably connected to the inner wall of the refrigerating cylinder, the side wall of the sliding disc is slidably connected to the inner wall of the refrigerating cylinder, and the side wall of the supporting circular plate is slidably connected to the inner wall of the clamping cylinder.

[0022] The present invention provides a nicotinic acid production equipment using nitrilase immobilization technology. Compared with the prior art, it has the following beneficial effects:

[0023] (1) The nitrilase immobilization technology production equipment for nicotinic acid production, when the nitrilase enters the interior of the immobilization box, the nitrilase can be immobilized and cleaned through the cooperation of the first drive motor, the transmission shaft, the stirring blade, the friction disk, the friction ring plate, the through hole, the heat preservation ring plate, the electric push rod and the sliding ring, so as to prevent the impurities adhering to the solid surface of the nitrilase and affecting its storage, and at the same time, the soft pad is set to prevent the nitrilase solid from being damaged when the stirring blade rotates at a high speed.

[0024] (2) The nicotinic acid production equipment using nitrilase immobilization technology can purify and filter the liquid in the immobilization box before it is discharged through the drain pipe, through the cooperation of the treatment box, purification filter and drain pipe, to avoid direct discharge and pollution of the environment. At the same time, the surface of the purification filter can be cleaned through the cooperation of the electric telescopic rod, cleaning brush, electric slide rail, sealing slide plate and garbage storage tank, to avoid impurities clogging the top of the purification filter and affecting its purification efficiency.

[0025] (3) The nitrilase immobilization technology production equipment for niacin production, when the nitrilase solid is stored in a refrigerated cylinder and turbid precipitation occurs, the nitrilase can be fixed and stirred by the cooperation of an electric rotating rod, a rotating disk and a fixed plate, without the need for workers to stir it manually, thereby reducing the labor intensity of the workers. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the structure of the nitrilase immobilization technology production equipment for nicotinic acid production of the present invention;

[0027] Figure 2 It is a structural schematic diagram of the immobilization mechanism of the present invention;

[0028] Figure 3 It is a structural schematic diagram of the processing mechanism of the present invention;

[0029] Figure 4 It is a schematic diagram of the internal structure of the processing box of the present invention;

[0030] Figure 5 It is a schematic diagram of the position structure of the electric push rod of the present invention;

[0031] Figure 6 It is a structural schematic diagram of the storage mechanism of the present invention;

[0032] Figure 7 It is a schematic diagram of the internal structure of the refrigeration cylinder of the present invention;

[0033] Figure 8 It is a schematic diagram of the internal structure of the chuck of the present invention.

[0034] In the figure: 1, immobilization box; 2, liquid inlet pipe; 3, cross-linking agent input pipe; 4, immobilization mechanism; 5, processing mechanism; 6, storage mechanism; 7, washing pipe; 40, first drive motor; 41, transmission shaft; 42, stirring blade; 43, friction disk; 44, cushion; 45, friction ring plate; 451, through hole; 46, insulation ring plate; 47, electric push rod; 48, sliding ring; 49, liquid discharge pipe; 491, discharge pipe; 50, processing box; 501, drainage pipe; 51, electric slide rail; 52, sealing slide plate; 53. Garbage storage tank; 54. Purification filter; 55. Humidity sensor; 56. Electric telescopic rod; 57. Telescopic plate; 58. Cleaning brush; 59. Inclined plate; 60. Second drive motor; 601. Rotating round rod; 602. Conveyor belt; 61. Clamping cylinder; 611. First limit spring; 612. First trigger switch; 62. Refrigeration cylinder; 63. Second trigger switch; 64. Second limit spring; 65. Sliding disc; 66. Electric rotating rod; 67. Rotating disc; 68. Fixed plate; 69. Solenoid valve. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Embodiment 1

[0037] See also Figure 1-Figure 2 , a nicotinic acid production nitrilase immobilization technology production equipment, comprising:

[0038] A fixed housing 1 and a fixed mechanism 4 arranged on the inner wall of the fixed housing 1;

[0039] A liquid inlet pipe 2, which is used to input the nitrilase liquid into the immobilization box 1;

[0040] A cross-linking agent input pipe 3, which is used to input the cross-linking agent into the immobilization box 1;

[0041] The processing mechanism 5 is used to purify and discharge the residual liquid;

[0042] A storage mechanism 6, the storage mechanism 6 is used to fix the nitrilase for frozen storage;

[0043] A washing pipe 7, which is used to input detergent into the interior of the immobilization box 1;

[0044] The bottom of the immobilization box 1 is fixedly connected to the top of the processing mechanism 5, the inner wall of the immobilization box 1 is rotatably connected to the side wall of the immobilization mechanism 4, the top of the immobilization box 1 is connected to the bottom of the liquid inlet pipe 2, the top of the immobilization box 1 is connected to the bottom of the cross-linking agent input pipe 3, the top of the immobilization box 1 is connected to the bottom of the washing pipe 7, and the inner wall and the side wall of the processing mechanism 5 are fixedly connected;

[0045] The immobilization mechanism 4 includes a first drive motor 40, the bottom of the first drive motor 40 is fixedly connected to the top of the immobilization box 1, the output end of the first drive motor 40 is fixedly connected to a transmission shaft 41, the side wall of the transmission shaft 41 is fixedly connected to a stirring blade 42, one side of the stirring blade 42 is fixedly connected to a friction disk 43, the inner wall of the immobilization box 1 is fixedly connected to a friction ring plate 45, a through hole 451 is opened inside the friction ring plate 45, the inner wall of the immobilization box 1 is fixedly connected to a heat preservation ring plate 46, the inner wall of the heat preservation ring plate 46 is fixedly connected to an electric push rod 47, the bottom of the electric push rod 47 is fixedly connected to a sliding ring 48, the bottom of the immobilization box 1 is connected to a drain pipe 49, and the bottom of the immobilization box 1 is connected to a discharge pipe 491;

[0046] The processing mechanism 5 includes a processing box 50, the bottom of the fixed box body 1 is fixedly connected to the top of the processing box 50, the side wall of the processing box 50 is fixedly connected to an electric slide rail 51, the electric slide rail 51 is slidably connected to a sealing slide plate 52 through an electronic slider, the inner wall of the processing box 50 is fixedly connected to a purification filter screen 54, and one side of the processing box 50 is connected to a drain pipe 501;

[0047] The storage mechanism 6 includes a conveyor belt 602, a side wall of the conveyor belt 602 is fixedly connected to the inner wall of the processing box 50, a rotating rod 601 is rotatably connected to the inner wall of the conveyor belt 602, a second driving motor 60 is fixedly connected to one side of the rotating rod 601, a clamping cylinder 61 is fixedly connected to the top of the conveyor belt 602, a first trigger switch 612 is fixedly connected to the top of the inner wall of the clamping cylinder 61, a first limit spring 611 is fixedly connected to the top of the inner wall of the clamping cylinder 61, a supporting circular plate 613 is fixedly connected to the top of the first limit spring 611, and a refrigeration cylinder 62 is movably connected to the top of the supporting circular plate 613;

[0048] The immobilization mechanism 4 further includes a cushion 44, the inner wall of the cushion 44 is fixedly connected to the side wall of the stirring blade 42, one side of the friction disc 43 is slidably connected to the inner wall of the friction ring plate 45, a filter is provided inside the through hole 451, the side wall of the sliding ring 48 is slidably connected to the inner wall of the heat preservation ring plate 46, the bottom of the first driving motor 40 is fixedly connected to the top of the immobilization box 1, and the transmission shaft 41 passes through the immobilization box 1;

[0049] It should be noted that the electric push rod 47 is started, and the electric push rod 47 drives the sliding ring 48 to press down to the bottom of the inner wall of the immobilization box 1, so as to seal and insulate the inside of the friction ring plate 45 to prevent the nitrilase from flowing out during immobilization. The staff inputs the nitrilase liquid and the cross-linking agent into the friction ring plate 45 through the liquid inlet pipe 2 and the cross-linking agent input pipe 3 respectively, and starts the first drive motor 40. The first drive motor 40 drives the friction disk 43 to rotate through the transmission shaft 41 and the stirring blade 42 to mix and stir the mixed liquid inside the friction ring plate 45. When the friction disk 43 rotates, it rubs against the inner wall of the friction ring plate 45 to generate heat so that the internal temperature of the friction ring plate 45 is maintained at about 40 degrees, which is in the optimal temperature environment for the reaction between the cross-linking agent and the nitrilase liquid, thereby increasing its reaction rate. When the nitrilase is immobilized, the electric push rod 47 is started to drive the sliding ring 48 to reset, and the drain pipe 49 is opened. , so that the excess liquid inside the friction ring plate 45 is discharged through the through hole 451 and the drain pipe 49, the stirring blade 42 drives the nitrilase solid inside the friction ring plate 45 to rotate, and the residual liquid on the surface of the nitrilase solid is thrown out by centrifugal force. The setting of the soft pad 44 prevents the stirring blade 42 from damaging the nitrilase solid when rotating at a high speed. When the liquid inside the immobilized box 1 is completely discharged, the drain pipe 49 is closed, and the staff inputs the detergent into the immobilized box 1 through the washing pipe 7 to clean the nitrilase solid. When the nitrilase solid is cleaned, the drain pipe 49 is opened, and the stirring blade 42 continues to drive the nitrilase to rotate fixedly and throw out the washing liquid by centrifugal force. When the washing liquid on the inner wall of the immobilized box 1 is completely discharged, the discharge pipe 491 is opened, and the nitrilase solid is discharged from the immobilized box 1, so as to avoid impurities adhering to the surface of the nitrilase solid and affecting its storage time.

[0050] Embodiment 2

[0051] See also Figure 3-Figure 5 , a nitrilase immobilization technology production equipment for nicotinic acid production, the processing mechanism 5 also includes a garbage storage tank 53, the side wall of the sealing slide plate 52 is fixedly connected to one side of the garbage storage tank 53, one side of the inner wall of the processing box 50 is fixedly connected to an electric telescopic rod 56, one side of the electric telescopic rod 56 is fixedly connected to a cleaning brush 58, one side of the cleaning brush 58 is fixedly connected to a telescopic plate 57, the bottom of the inner wall of the processing box 50 is fixedly connected to a humidity sensor 55, the top of the cleaning brush 58 is fixedly connected to an inclined plate 59, one side of the inclined plate 59 is fixedly connected to one side of the inner wall of the processing box 50, one side of the telescopic plate 57 is fixedly connected to one side of the inner wall of the processing box 50, the bottom of the cleaning brush 58 is slidably connected to the top of the purification filter 54, and one side of the sealing slide plate 52 is slidably connected to the side wall of the processing box 50;

[0052] It should be noted that when the washing liquid and the mixed liquid are discharged into the interior of the treatment box 50 through the drain pipe 49, the liquid is purified and filtered through the setting of the purification filter 54 to prevent its direct discharge from polluting the surrounding environment. The humidity at the bottom of the inner wall of the treatment box 50 is monitored in real time through the setting of the humidity sensor 55. When the top of the purification filter 54 is blocked by liquid impurities, the liquid cannot pass through the purification filter 54. The humidity sensor 55 controls the electric slide rail 51 to start, and the electric slide rail 51 drives the sealing slide plate 52 to press down and stop sealing the treatment box 50. The electric telescopic rod 56 drives the cleaning brush 58 to move to clean and push out the impurities accumulated on the top of the purification filter 54. The liquid impurities are collected and stored through the setting of the garbage storage slot 53. The liquid impurities are prevented from entering the bottom of the telescopic plate 57 through the setting of the inclined plate 59 and the telescopic plate 57.

[0053] Embodiment 3

[0054] See also Figure 6-Figure 8 , a nitrilase immobilization technology production equipment for nicotinic acid production, the storage mechanism 6 also includes a second trigger switch 63, the bottom of the inner wall of the refrigeration cylinder 62 is fixedly connected to the bottom of the second trigger switch 63, the bottom of the inner wall of the refrigeration cylinder 62 is fixedly connected to a second limit spring 64, the top of the second limit spring 64 is fixedly connected to a sliding disc 65, the top of the sliding disc 65 is fixedly connected to an electric rotating rod 66, the top of the electric rotating rod 66 is fixedly connected to a rotating disc 67, the top of the rotating disc 67 is fixedly connected to a fixed plate 68, the second trigger switch 63 is provided with a solenoid valve 69, one side of the fixed plate 68 is slidably connected to the inner wall of the refrigeration cylinder 62, the side wall of the sliding disc 65 is slidably connected to the inner wall of the refrigeration cylinder 62, and the side wall of the support circular plate 613 is slidably connected to the inner wall of the clamping cylinder 61;

[0055] It should be noted that when the immobilization box 1 is immobilizing the nitrilase liquid, the second drive motor 60 is started, and the second drive motor 60 drives the solenoid valve 69 to move to the bottom of the discharge pipe 491 through the rotating rod 601, the conveyor belt 602, the clamping cylinder 61 and the supporting circular plate 613. When the solid is discharged through the discharge pipe 491, it enters the interior of the refrigeration cylinder 62. The nitrilase solid drives the rotating disc 67 downward through the influence of gravity. The rotating disc 67 drives the sliding disc 65 downward through the electric rotating rod 66 until the sliding disc 65 hits the top of the second trigger switch 63. The second trigger switch 63 controls the discharge pipe 491 and the solenoid valve 69 to close, so as to avoid excessive storage of nitrilase in the refrigeration cylinder 62. Enzyme solids, causing them to spill. When the inner wall of the refrigeration cylinder 62 stores enough nitrilase solids, the support circular plate 613 is pressed down by gravity, and the support circular plate 613 hits the top of the first trigger switch 612. The first trigger switch 612 controls the second drive motor 60 to start. The second drive motor 60 drives the refrigeration cylinder 62 to move to the bottom of the discharge pipe 491 through the new clamping cylinder 61 to continue to collect and store the nitrilase solids. When the nitrilase solids stored in the refrigeration cylinder 62 appear turbid precipitation, the electric rotating rod 66 is started. The electric rotating rod 66 drives the fixed plate 68 to rotate through the rotating disc 67 to stir the nitrilase solids in the refrigeration cylinder 62 to avoid turbid precipitation.

[0056] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0057] During operation, the electric push rod 47 is started, and the electric push rod 47 drives the sliding ring 48 to press down to the bottom of the inner wall of the immobilization box 1, and the inside of the friction ring plate 45 is sealed and insulated to prevent the nitrilase from flowing out during immobilization. The staff inputs the nitrilase liquid and the cross-linking agent into the friction ring plate 45 through the liquid inlet pipe 2 and the cross-linking agent input pipe 3 respectively, and starts the first drive motor 40. The first drive motor 40 drives the friction disk 43 to rotate through the transmission shaft 41 and the stirring blade 42 to mix and stir the mixed liquid inside the friction ring plate 45. When the friction disk 43 rotates, it rubs against the inner wall of the friction ring plate 45 to generate heat so that the internal temperature of the friction ring plate 45 is maintained at about 40 degrees, which is in the optimal temperature environment for the reaction between the cross-linking agent and the nitrilase liquid, thereby increasing its reaction rate. When the nitrilase is immobilized, the electric push rod 47 is started to drive the sliding ring 48 to reset, and the drain pipe 49 is opened. The excess liquid in the friction ring plate 45 is discharged through the through hole 451 and the drain pipe 49, the stirring blade 42 drives the nitrilase solid in the friction ring plate 45 to rotate, and the residual liquid on the surface of the nitrilase solid is thrown out by centrifugal force. The setting of the soft pad 44 prevents the nitrilase solid from being damaged when the stirring blade 42 rotates at a high speed. When the liquid in the immobilized box 1 is completely discharged, the drain pipe 49 is closed, and the staff inputs the detergent into the immobilized box 1 through the washing pipe 7 to clean the nitrilase solid. When the nitrilase solid is cleaned, the drain pipe 49 is opened, and the stirring blade 42 continues to drive the nitrilase to rotate fixedly and throw out the washing liquid by centrifugal force. When the washing liquid on the inner wall of the immobilized box 1 is completely discharged, the discharge pipe 491 is opened, and the nitrilase solid is discharged from the immobilized box 1, so as to avoid impurities adhering to the surface of the nitrilase solid and affecting its storage time.

[0058] When the washing liquid and the mixed liquid are discharged into the processing box 50 through the drain pipe 49, the liquid is purified and filtered by the setting of the purification filter 54 to prevent it from being directly discharged and causing pollution to the surrounding environment. The humidity at the bottom of the inner wall of the processing box 50 is monitored in real time by the setting of the humidity sensor 55. When the top of the purification filter 54 is blocked by liquid impurities, the liquid cannot pass through the purification filter 54. The humidity sensor 55 controls the electric slide rail 51 to start, and the electric slide rail 51 drives the sealing slide plate 52 to press down and stop sealing the processing box 50. The electric telescopic rod 56 drives the cleaning brush 58 to move to clean and push out the impurities accumulated on the top of the purification filter 54. The liquid impurities are collected and stored by the setting of the garbage storage tank 53. The liquid impurities are prevented from entering the bottom of the telescopic plate 57 by the setting of the inclined plate 59 and the telescopic plate 57.

[0059] When the immobilization box 1 is immobilizing the nitrilase liquid, the second drive motor 60 is started. The second drive motor 60 drives the solenoid valve 69 to move to the bottom of the discharge pipe 491 through the rotating rod 601, the conveyor belt 602, the clamping cylinder 61 and the supporting circular plate 613. When the solid is discharged through the discharge pipe 491, it enters the interior of the refrigeration cylinder 62. The nitrilase solid drives the rotating disc 67 downward under the influence of gravity. The rotating disc 67 drives the sliding disc 65 downward through the electric rotating rod 66 until the sliding disc 65 hits the top of the second trigger switch 63. The second trigger switch 63 controls the discharge pipe 491 and the solenoid valve 69 to close, so as to avoid excessive storage of nitrilase solids in the refrigeration cylinder 62. When the inner wall of the refrigerating cylinder 62 stores enough nitrilase solids, the supporting circular plate 613 is pressed down by gravity, and the supporting circular plate 613 hits the top of the first trigger switch 612. The first trigger switch 612 controls the second drive motor 60 to start, and the second drive motor 60 drives the refrigerating cylinder 62 to move to the bottom of the discharge pipe 491 through the new clamping cylinder 61 to continue to collect and store the nitrilase solids. When the nitrilase solids stored in the refrigerating cylinder 62 become turbid and precipitate, the electric rotating rod 66 is started, and the electric rotating rod 66 drives the fixed plate 68 to rotate by the rotating disc 67 to stir the nitrilase solids in the refrigerating cylinder 62 to avoid turbid precipitation.

[0060] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0061] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nicotinic acid production equipment using nitrilase immobilization technology, characterized in that: include: A fixation box (1), and a fixation mechanism (4) arranged on the inner wall of the fixation box (1); A liquid inlet pipe (2), the liquid inlet pipe (2) is used to input the nitrilase liquid into the interior of the immobilization box (1); A cross-linking agent input pipe (3), the cross-linking agent input pipe (3) is used to input the cross-linking agent into the interior of the immobilization box (1); A processing mechanism (5), the processing mechanism (5) is used to purify and discharge the residual liquid; A storage mechanism (6), the storage mechanism (6) is used to fix the nitrilase for frozen storage; A washing pipe (7), the washing pipe (7) being used to input detergent into the interior of the immobilization box (1); The bottom of the immobilization box (1) is fixedly connected to the top of the processing mechanism (5), the inner wall of the immobilization box (1) is rotatably connected to the side wall of the immobilization mechanism (4), the top of the immobilization box (1) is connected to the bottom of the liquid inlet pipe (2), the top of the immobilization box (1) is connected to the bottom of the cross-linking agent input pipe (3), the top of the immobilization box (1) is connected to the bottom of the washing pipe (7), and the inner wall of the processing mechanism (5) is fixedly connected to the side wall; The immobilization mechanism (4) comprises a first drive motor (40), the bottom of the first drive motor (40) is fixedly connected to the top of the immobilization box (1), the output end of the first drive motor (40) is fixedly connected to a transmission shaft (41), the side wall of the transmission shaft (41) is fixedly connected to a stirring blade (42), one side of the stirring blade (42) is fixedly connected to a friction disk (43), the inner wall of the immobilization box (1) is fixedly connected to a friction ring plate (45), the friction ring plate (45) is provided with a through hole (451) inside; the inner wall of the immobilization box (1) is fixedly connected to a heat preservation ring plate (46), the inner wall of the heat preservation ring plate (46) is fixedly connected to an electric A push rod (47), the bottom of the electric push rod (47) is fixedly connected to a sliding ring (48), the bottom of the immobilized box (1) is connected to a liquid discharge pipe (49), and the bottom of the immobilized box (1) is connected to a material discharge pipe (491); the side wall of the stirring blade (42) is fixedly connected to a soft pad (44), one side of the friction disk (43) is slidably connected to the inner wall of the friction ring plate (45), a filter screen is provided inside the through hole (451), the side wall of the sliding ring (48) is slidably connected to the inner wall of the heat preservation ring plate (46), the bottom of the first drive motor (40) is fixedly connected to the top of the immobilized box (1), and the transmission shaft (41) passes through the immobilized box (1); The processing mechanism (5) comprises a processing box (50), the bottom of the fixed box body (1) is fixedly connected to the top of the processing box (50), the side wall of the processing box (50) is fixedly connected to an electric slide rail (51), the electric slide rail (51) is slidably connected to a sealing slide plate (52) via an electronic slide block, the inner wall of the processing box (50) is fixedly connected to a purification filter screen (54), and one side of the processing box (50) is connected to a drainage pipe (501); The storage mechanism (6) comprises a conveyor belt (602), the side wall of the conveyor belt (602) is fixedly connected to the inner wall of the processing box (50), the inner wall of the conveyor belt (602) is rotatably connected to a rotating round rod (601), one side of the rotating round rod (601) is fixedly connected to a second driving motor (60), and the top of the conveyor belt (602) is fixedly connected to a clamping cylinder (61).

2. The nitrilase immobilization technology production equipment for nicotinic acid production according to claim 1, characterized in that: The processing mechanism (5) further comprises a garbage storage tank (53), a side wall of the sealing slide plate (52) being fixedly connected to one side of the garbage storage tank (53), an electric telescopic rod (56) being fixedly connected to one side of the inner wall of the processing box (50), a cleaning brush (58) being fixedly connected to one side of the electric telescopic rod (56), a telescopic plate (57) being fixedly connected to one side of the cleaning brush (58), a humidity sensor (55) being fixedly connected to the bottom of the inner wall of the processing box (50), and an inclined plate (59) being fixedly connected to the top of the cleaning brush (58).

3. The nitrilase immobilization technology production equipment for nicotinic acid production according to claim 2, characterized in that: One side of the inclined plate (59) is fixedly connected to one side of the inner wall of the processing box (50), one side of the telescopic plate (57) is fixedly connected to one side of the inner wall of the processing box (50), the bottom of the cleaning brush (58) is slidably connected to the top of the purification filter (54), and one side of the sealing slide plate (52) is slidably connected to the side wall of the processing box (50).

4. The nitrilase immobilization technology production equipment for nicotinic acid production according to claim 1, characterized in that: The top of the inner wall of the clamping cylinder (61) is fixedly connected to a first trigger switch (612), the top of the inner wall of the clamping cylinder (61) is fixedly connected to a first limit spring (611), the top of the first limit spring (611) is fixedly connected to a supporting circular plate (613), the top of the supporting circular plate (613) is movably connected to a refrigeration cylinder (62), the bottom of the inner wall of the refrigeration cylinder (62) is fixedly connected to the bottom of the second trigger switch (63), the bottom of the inner wall of the refrigeration cylinder (62) is fixedly connected to a second limit spring (64), the top of the second limit spring (64) is fixedly connected to A sliding disc (65) is connected, the top of the sliding disc (65) is fixedly connected to an electric rotating rod (66), the top of the electric rotating rod (66) is fixedly connected to a rotating disc (67), the top of the rotating disc (67) is fixedly connected to a fixed plate (68), the second trigger switch (63) is provided with a solenoid valve (69), one side of the fixed plate (68) is slidably connected to the inner wall of the refrigeration cylinder (62), the side wall of the sliding disc (65) is slidably connected to the inner wall of the refrigeration cylinder (62), and the side wall of the supporting circular plate (613) is slidably connected to the inner wall of the clamping cylinder (61).

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