Method for repairing and improving diseased soil by bacteria detection and fertilization and application of method

Abstract

The invention relates to the technical field of agriculture, and particularly discloses a bacteria testing and fertilization repairing and improving method for diseased soil and application of the bacteria testing and fertilization repairing and improving method. The bacteria testing and fertilizing repairing and improving method for the diseased soil comprises the following steps that (1) in October of the current year, the content of organic matter in the diseased soil is detected, and the dosage of a soil conditioner is determined; the soil conditioner is applied to the diseased soil in November; (2) in the first ten days of May of the next year, detecting the relative abundance of fungal and bacterial level pathogenic bacteria in normal soil and diseased soil, and determining the dosage of a microbial agent; the microbial agent is applied to the diseased soil in the middle of May. According to the bacteria testing and fertilization repairing and improving method for the diseased soil, the microbial structural difference between the diseased soil and normal soil in the crop growth process is detected and analyzed, the dosage of the microbial agent is dynamically adjusted, and therefore a microbial system of the diseased soil is improved, and repairing of continuous cropping obstacle soil is achieved.

Description

Technical Field

[0001] The present application relates to the field of agricultural technology, and in particular to a method for improving diseased soil by testing bacteria and applying fertilizers, and its application. Background Art

[0002] Continuous cropping refers to the phenomenon that when the same crop or closely related crops are planted in the same soil for many consecutive years, even under normal cultivation and management conditions, the plants will grow weaker, the yield will decrease, and the quality will decline. The main reason is that continuous cropping for many years leads to changes in the physical and chemical properties of the soil, as well as light, temperature, humidity, and gas. The growth of some beneficial microorganisms is inhibited, while some harmful microorganisms are reproduced, the natural balance of soil microorganisms is destroyed, and the problems of soil wilt, stem base rot, root rot and other diseases are getting worse year by year.

[0003] In response to soil problems caused by crop continuous cropping, producers currently mainly use soil testing and fertilization technology to improve the soil, that is, according to the nutrients required by the crop growth stage and the fertilizer supply performance of the growth environment, the mineral nutrients such as nitrogen, phosphorus and potassium are balanced in the planting soil; however, the above method can only reduce or alleviate the degree of crop disease caused by soil damage, and this method only studies the relationship between land, crops and fertilizers, and does not study the impact of changes in soil microbial community structure and crop growth. Therefore, it cannot fundamentally solve the problem of soil continuous cropping.

[0004] Therefore, it is of great significance to study a soil remediation and improvement method that can dynamically grasp the changes in soil microbial community structure in the problem of continuous cropping, and use fertilizers, soil conditioners and microbial agents in a targeted manner, in order to improve the quality of continuous cropping crops. Summary of the invention

[0005] In order to fundamentally solve the soil problems caused by continuous cropping and improve the yield and quality of continuous cropping crops, the present application provides a method for improving diseased soil by testing bacteria and fertilizing and its application.

[0006] The present application provides a method for improving diseased soil by testing bacteria and fertilizing, comprising the following steps: (1) In October of the same year, test the organic matter content in the diseased soil and determine the amount of soil conditioner to be used; in November, apply the soil conditioner to the diseased soil; (2) In early May of the following year, the relative abundance of pathogenic bacteria at the level of fungi and bacteria in normal soil and diseased soil is detected to determine the dosage of the microbial agent; in mid-May, the microbial agent is applied to the diseased soil; the dosage of the microbial agent is determined as follows: When the relative abundance is less than 5%, the dosage of microbial agent is 1.8-2.2kg / mu. When the relative abundance is 5-10%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 10%, the dosage of microbial agents is 4.5-5.5 kg / mu.

[0007] The present application provides a method for improving the repair of diseased soil by testing and fertilizing bacteria. This method adds the technology of testing and fertilizing bacteria to the traditional soil testing and fertilization, that is, by analyzing the changes in the soil microbial community during crop growth, comparing the differences in the microbial structure between diseased soil and normal soil, and dynamically adjusting the dosage of microbial agents, the microbial system of the diseased soil is improved, and the repair of soil that is hindered by continuous cropping is achieved. In other words, the present application effectively combines soil testing and fertilization with testing and fertilization bacteria, and uses soil conditioners and microbial agents in a targeted and dynamic manner, thereby improving the physical and chemical indicators of the soil, improving the soil microecological system, and thus improving the quality of continuous cropping crops.

[0008] In the present application, May is the first period when soil-borne diseases of ginger occur. During this period, testing and fertilizing can inhibit the reproduction of harmful microorganisms in the diseased soil, and initially achieve effective prevention and control of diseased ginger soil. Optionally, the method for repairing and improving diseased soil by testing and fertilizing also includes: (3) in early June of the following year, detecting the organic matter content in the diseased soil, and the relative abundance of pathogenic bacteria at the level of fungi and bacteria in normal soil and diseased soil, and determining the amount of soil conditioner and microbial agent; applying the soil conditioner and microbial agent to the diseased soil in mid-June; the amount of microbial agent is determined as follows: When the relative abundance is less than 5%, no microbial agents are needed. When the relative abundance is 10-20%, the dosage of microbial agent is 1.8-2.2kg / mu. When the relative abundance is 20-30%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 30%, the dosage of microbial agents is 4.5-5.5 kg / mu.

[0009] In the present application, the agricultural operation of soiling ginger is usually carried out in June. Therefore, during this period, soil conditioners and microbial agents are used in combination and applied to the ginger soil. On the one hand, the soil can be improved; on the other hand, the soil conditioners can provide necessary nutrients for the survival of microorganisms, thereby achieving effective prevention and control of diseases. Optionally, the method for repairing and improving diseased soil by testing bacteria and applying fertilizers also includes: (4) in early July of the following year, detecting the relative abundance of pathogenic bacteria at the level of fungi and bacteria in normal soil and diseased soil, and determining the amount of microbial agent; applying the microbial agent to the diseased soil in mid-July; the amount of the microbial agent is determined as follows: When the relative abundance is less than 10%, no microbial agents are needed. When the relative abundance is 10-20%, the dosage of microbial agent is 1.8-2.2kg / mu. When the relative abundance is 20-30%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 30%, the dosage of microbial agents is 4.5-5.5 kg / mu.

[0010] In this application, July has entered a period of high temperature and high humidity, which is the second peak period for soil-borne diseases of ginger. Therefore, during this period, the soil is tested for bacteria and fertilized to further inhibit the reproduction of harmful microorganisms and avoid the occurrence of diseased plants such as stem wilting, dry leaves, stem base rot and seedling death in ginger, thereby further ensuring the yield and quality of ginger.

[0011] Optionally, in step (1), the amount of the soil conditioner is determined as follows: When organic matter is less than 15g / kg, soil conditioner is 600kg / mu. When organic matter is 16-25g / kg and soil conditioner is 400kg / mu, When organic matter is 26-35g / kg and soil conditioner is 320kg / mu, When organic matter is greater than 36g / kg, soil conditioner is 200kg / mu.

[0012] Optionally, in step (3), the amount of the soil conditioner is determined as follows: When organic matter is less than 15g / kg, soil conditioner is 200kg / mu. When organic matter is 16-25g / kg and soil conditioner is 160kg / mu, When organic matter is 26-35g / kg and soil conditioner is 120kg / mu, When organic matter is greater than 36g / kg, soil conditioner is 80kg / mu.

[0013] Optionally, the viable bacteria count of the microbial agent is ≥ 500 million CFU / g, and the strain is selected from one or more of Bacillus velez, Bacillus atrophaeus and Trichoderma.

[0014] Optionally, the microbial agent contains Bacillus Velezii, Bacillus atrophaeus and Trichoderma, and the number of viable bacteria is ≥ 500 million CFU / g; the microbial agent is used by drip irrigation or flushing.

[0015] Optionally, in the microbial agent, the ratio of the number of live bacteria of Bacillus Velez, Bacillus atrophaeus and Trichoderma is 2:(0.3-1):(1-1.8).

[0016] In some embodiments, the ratio of the viable cell counts of Bacillus Velezii, Bacillus atrophaeus and Trichoderma can be 2:(0.3-0.5):1.2, 2:(0.3-0.8):1.2, 2:(0.3-1):1.2, 2:(0.5-0.8):1.2, 2:(0.5-1):1.2, 2:(0.8-1):1.2, 2:0.5:(1-1.2), 2:0.5:(1-1.5), 2:0.5:(1-1.8), 2:0.5:(1.2-1.5), 2:0.5:(1.2-1.8) or 2:0.5:(1.5-1.8).

[0017] In a specific embodiment, the ratio of the number of live bacteria of Bacillus Velez, Bacillus atrophaeus and Trichoderma can also be 2:0.3:1.2, 2:0.5:1.2, 2:0.8:1.2, 2:1:1.2, 2:0.5:1, 2:0.5:1.5 or 2:0.5:1.8.

[0018] Optionally, the soil conditioner is applied in step (1) by evenly spreading the soil conditioner on the soil surface and then rotary tilling the soil to a depth of 25-50 cm; The method for applying the soil conditioner in step (3) is: evenly spreading the soil conditioner on the soil surface and then covering the soil.

[0019] Optionally, the soil conditioner has a live bacterial count of ≥200 million CFU / g, an organic matter content of ≥≥75%, a total biological humic acid of ≥30%, an easily oxidizable organic matter of ≥20%, and calcium+magnesium+iron+manganese+zinc+iodine of ≥400 mg / kg.

[0020] Optionally, the diseased soil is diseased soil caused by continuous cropping of ginger.

[0021] In summary, this application has the following beneficial effects: 1. The present application provides an improved method for repairing diseased soil by testing and fertilizing. The method adopts the technology of testing and fertilizing. By measuring the microbial structure of the soil during crop growth and dynamically applying microbial agents, the disease problem of soil caused by continuous cropping is solved and soil repair caused by continuous cropping is achieved.

[0022] 2. The present application adopts a microbial agent comprising Bacillus Velez, Bacillus atrophaeus and Trichoderma to repair the soil of ginger continuous cropping, and controls the number of live bacteria of the three in the microbial agent within the range of 2: (0.3-1): (1-1.8), which can achieve better repair effect of the soil of ginger continuous cropping obstacles, better quality of ginger and higher yield. DETAILED DESCRIPTION

[0023] The present application provides a method for improving the soil disease of ginger by testing bacteria and fertilizing, comprising the following steps: (1) In October of the same year, the organic matter content in the diseased ginger soil was detected and the amount of soil conditioner was determined; in November, the soil conditioner was evenly spread on the surface of the diseased ginger soil, and then rotary tillage was performed to a depth of 25-50 cm; the amount of the soil conditioner was determined as follows: When organic matter is less than 15g / kg, soil conditioner is 600kg / mu. When organic matter is 16-25g / kg and soil conditioner is 400kg / mu, When organic matter is 26-35g / kg and soil conditioner is 320kg / mu, When organic matter is greater than 36g / kg, soil conditioner is 200kg / mu.

[0024] (2) In early May of the following year, the relative abundance of fungi and bacteria in normal soil and diseased ginger soil was detected to determine the dosage of the microbial agent; in mid-May, the microbial agent was drip-irrigated or flushed into the diseased ginger soil; the dosage of the microbial agent was determined as follows: When the relative abundance is less than 5%, the dosage of microbial agent is 1.8-2.2kg / mu. When the relative abundance is 5-10%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 10%, the dosage of microbial agents is 4.5-5.5 kg / mu.

[0025] (3) In early June of the following year, the organic matter content in the diseased ginger soil and the relative abundance of fungal and bacterial pathogens in the normal soil and the diseased ginger soil were detected to determine the dosage of soil conditioner and microbial agent; in late June, the soil conditioner was evenly spread on the soil surface and then covered with soil, and the microbial agent was drip-irrigated or flushed into the diseased ginger soil; The dosage of the microbial agent is determined as follows: When the relative abundance is less than 5%, no microbial agents are needed. When the relative abundance is 10-20%, the dosage of microbial agent is 1.8-2.2kg / mu. When the relative abundance is 20-30%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is >30%, the dosage of microbial agent is 4.5-5.5kg / mu; The amount of soil conditioner used is determined as follows: When organic matter is less than 15g / kg, soil conditioner is 200kg / mu. When organic matter is 16-25g / kg and soil conditioner is 160kg / mu, When organic matter is 26-35g / kg and soil conditioner is 120kg / mu, When organic matter is greater than 36g / kg, soil conditioner is 80kg / mu.

[0026] (4) In early July of the following year, the relative abundance of fungi and bacteria in normal soil and diseased ginger soil was detected to determine the dosage of the microbial agent; in mid-July, the microbial agent was drip-irrigated or flushed into the diseased ginger soil; the dosage of the microbial agent was determined as follows: When the relative abundance is less than 10%, no microbial agents are needed. When the relative abundance is 10-20%, the dosage of microbial agent is 1.8-2.2kg / mu. When the relative abundance is 20-30%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 30%, the dosage of microbial agent is 4.5-5.5 kg / mu.

[0027] In the present application, the number of viable bacteria of the microbial agent is ≥ 500 million CFU / g, and the strain is selected from one or more of Bacillus Velez, Bacillus atrophaeus and Trichoderma. Further, the microbial agent contains Bacillus Velez, Bacillus atrophaeus and Trichoderma with a ratio of viable bacteria of 2: (0.3-1): (1-1.8); further, the ratio of viable bacteria of Bacillus Velez, Bacillus atrophaeus and Trichoderma is 2: (0.3-0.8): (1.2-1.5).

[0028] In this application, the community structure of fungi and bacteria in normal soil and diseased soil was analyzed using high-throughput sequencing technology, and the above-mentioned detection was entrusted to Beijing Novogene Technology Co., Ltd.; the method for detecting the organic matter content of the soil was carried out in accordance with NY / T1121.6-2006.

[0029] In the present application, the deposit number of Bacillus velez is CGMCC No.28824; the deposit number of Bacillus atrophaeus is CGMCC No.29366; the deposit number of Trichoderma is CGMCC No.15677; the soil conditioner is a commercially available product of Beijing Jiabowen Biotechnology Co., Ltd., and the number of viable bacteria in the soil conditioner is ≥200 million CFU / g, the organic matter content is ≥75%, the total biological humic acid is ≥30%, the easily oxidized organic matter is ≥20%, calcium + magnesium + iron + manganese + zinc + iodine is ≥400 mg / kg, pH: 6.5-7.5, and the moisture is ≤8%; other reagents, solvents, etc. used in the present application can also be obtained commercially.

[0030] The present application is further described in detail below in conjunction with embodiments and energy detection tests.

[0031] Example 1 Example 1 provides a method for improving soil disease in ginger by testing bacteria and applying fertilizers, comprising the following steps: (1) In October 2023, two ginger planting test fields of equal area were selected in Xihetan Village, Changyi City, Weifang City, Shandong Province, and the test crop Changyi ginger (planting density: 4000-4500 trees / mu) was planted in the test fields; one of them was a normal test field without continuous cropping obstacles, and the other was a disease test field with relatively serious continuous cropping obstacles①; Diseased soil was taken from the disease test field ①. The organic matter content in the diseased soil ① was tested to be 17.2 g / kg, and the amount of soil conditioner was determined to be 400 kg / mu. In November, the soil conditioner was evenly spread in the disease test field ① according to the above amount, and then rotary tillage was performed, and the depth of rotary tillage was 35±5 cm. (2) On May 1, 2023, soil was collected from the normal test field and the disease test field ①, and the relative abundance of fungal and bacterial pathogens in the two soils was tested. The relative abundance of the diseased soil ① was 13.4% compared with the normal soil, and the dosage of the microbial agent was determined to be 5 kg / mu; on May 15, the microbial agent was diluted 500 times according to the above dosage and then flushed into the diseased test field ①; (3) On June 5, 2023, soil was collected from the normal test field and the diseased test field ①, and the relative abundance of fungal and bacterial pathogens in the two soils was tested. The relative abundance of the diseased soil ① was 11.4% compared with the normal soil, and the dosage of the microbial agent was determined to be 2 kg / mu; the organic matter content in the diseased soil ① was 30.4 g / kg, and the dosage of the soil conditioner was determined to be 120 kg / mu; On June 13, the soil conditioner was evenly spread in the disease test field① according to the above dosage, and then covered with soil; the microbial agent was diluted 500 times according to the above dosage and flushed into the disease test field; (4) On July 8, 2023, soil was collected from the normal test field and the disease test field ①, and the relative abundance of pathogenic bacteria at the fungal and bacterial levels in the two soils was tested. The relative abundance of the diseased soil ① was 7.8% compared to the normal soil, so there was no need to apply microbial agents; In the above embodiment, the viable bacteria count of the microbial agent is 500 million CFU / g, and the ratio of the viable bacteria counts of Bacillus velezensis, Bacillus atrophaeus and Trichoderma is 2:0.5:1.2.

[0032] Example 2 Example 2 provides a method for improving soil disease in ginger by testing bacteria and applying fertilizers, comprising the following steps: (1) In October 2023, two ginger planting test fields of equal area were selected in Dalianying Village, Changyi City, Weifang City, Shandong Province, and the test crop Changyi ginger (planting density: 4000-4500 trees / mu) was planted in the test fields; one of them was a normal test field without continuous cropping obstacles, and the other was a disease test field with relatively serious continuous cropping obstacles②; Diseased soil was taken from the disease test field ②. The organic matter content in the diseased soil ② was tested to be 27.4 g / kg, and the amount of soil conditioner was determined to be 320 kg / mu. In November, the soil conditioner was evenly spread on the soil surface of the disease test field according to the above amount, and then rotary tillage was performed, and the depth of rotary tillage was 35±5 cm. (2) On May 1, 2023, soil was collected from the normal test field and the disease test field, and the relative abundance of fungal and bacterial pathogens in the two soils was tested. The relative abundance of the diseased soil was 20.7% compared with the normal soil, and the dosage of the microbial agent was determined to be 5 kg / mu; on May 15, the microbial agent was diluted 500 times according to the above dosage and then flushed into the diseased test field; (3) On June 5, 2023, soil was collected from the normal test field and the diseased test field, and the relative abundance of fungal and bacterial pathogens in the two soils was tested. The relative abundance of the diseased soil was 16.8% compared with the normal soil, and the dosage of the microbial agent was determined to be 2 kg / mu; the organic matter content in the diseased soil was 39.1 g / kg, and the dosage of the soil conditioner was determined to be 80 kg / mu; On June 13, the soil conditioner was evenly spread in the disease test field according to the above dosage, and then covered with soil; the microbial agent was diluted 500 times according to the above dosage and flushed in the disease test field; (4) On July 8, 2023, soil was collected from the normal test field and the disease test field, and the relative abundance of fungal and bacterial pathogens in the two soils was detected. The relative abundance of the diseased soil was 11.3% compared with the normal soil, and the dosage of the microbial agent was determined to be 2 kg / mu; on July 12, the microbial agent was diluted 500 times according to the above dosage and flushed into the diseased test field; In the above embodiment, the viable bacteria count of the microbial agent is 500 million CFU / g, and the ratio of the viable bacteria counts of Bacillus velez, Bacillus atrophaeus and Trichoderma is 2:0.5:1.2.

[0033] Example 3-11 Embodiments 3-11 respectively provide a method for improving the restoration of diseased ginger soil by testing bacteria and applying fertilizers.

[0034] The difference between the above embodiment and embodiment 1 is that the types of strains and the ratio of the number of viable bacteria in the microbial inoculant are specifically shown in Table 1 below.

[0035] Table 1 Types of microbial strains and ratios of viable counts in Example 1 and Examples 3-11 Performance testing (1) On August 2, 2023, the quality of ginger in the experimental fields improved by the methods of Examples 1-11 (demonstration areas 1-11), the diseased experimental field ① (control area 1) that was not repaired and improved, the diseased experimental field ② (control area 2) that was not repaired and improved, and the normal experimental field (control area 3) were tested, including plant height and head stem thickness, and the average values ​​were taken. The results are shown in Table 2 below.

[0036] (2) On August 2, 2023, the ginger plants in each experimental field were observed to see if they had diseased plants such as stem wilting, dry leaves, and dead seedlings due to stem base rot. The number of diseased plants in each area was counted. The results are shown in Table 2 below.

[0037] (3) On October 17, 2023, the ginger from each experimental field was harvested, the dirt on the surface of the ginger was washed with water, and the ginger yield of each experimental field was obtained. The results are shown in Table 2 below.

[0038] Table 2 Quality and yield test results of ginger in each test field According to the test results in Table 2, the ginger plants in control area 1 and control area 2 had a large number of withered dry leaves, stem base rot and dead seedlings, the number of diseased plants was 1278-1591 plants / mu, and the yield was about 2416.9-2657.4 kg / mu; while the ginger plants in demonstration areas 1-11 grew vigorously, had green leaves, and had very few dead seedlings, the number of diseased plants was only 57-373 plants / mu, and the yield was as high as 4830.8-5736.1 kg / mu. Therefore, it is shown that the method for repairing and improving the diseased soil of ginger with bacteria and fertilization provided in this application can repair the soil problems that hinder the continuous cropping of ginger and effectively improve the quality and yield of continuous cropping of ginger.

[0039] The test results of Demonstration Area 1 and Demonstration Area 3-11 showed that in Demonstration Area 1 and Demonstration Area 3-8, microbial agents containing Bacillus Velez, Bacillus atrophaeus and Trichoderma with a live bacteria count ratio of 2: (0.3-1): (1-1.8) were used to improve the diseased soil ①, and the obtained ginger plant height was about 133.3-138.6cm, the head stem diameter was 20.0-21.8mm, the number of diseased plants was 57-219 plants / mu, and the yield was 5025.2-5736.1kg / mu; while in Demonstration Area 9, microbial agents containing Bacillus Velez, Bacillus atrophaeus and Trichoderma with a live bacteria count ratio of 1:1:1 were used to improve the diseased soil ①. The plant height of the improved ginger was about 128.6cm, the diameter of the head stem was 20.0mm, the number of diseased plants was 234 plants / mu, and the yield was 4934.9kg / mu; the demonstration area 10-11 used a microbial agent containing Bacillus Velez and Bacillus atrophaeus with a live bacteria ratio of 2:0.5, or a microbial agent containing Bacillus Velez and Trichoderma with a live bacteria ratio of 2:1.2 to improve the diseased soil①, and the plant height of the obtained ginger was about 125.6-127.7cm, the diameter of the head stem was 19.1-19.3mm, the number of diseased plants was 289-373 plants / mu, and the yield was 4830.8-4885.7kg / mu. Therefore, it is explained that the present application further uses a microbial agent comprising Bacillus Velezii, Bacillus atrophaeus and Trichoderma with a live bacteria count ratio of 2: (0.3-1): (1-1.8) to improve the diseased soil, which can achieve a better repair effect on the soil that is hindered by continuous cropping of ginger, better quality of ginger and higher yield.

[0040] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A method for improving diseased soil by testing bacteria and fertilizing, characterized in that: The following steps are involved: (1) In October of the same year, test the organic matter content in the diseased soil and determine the amount of soil conditioner to be used; in November, apply the soil conditioner to the diseased soil; (2) In early May of the following year, the relative abundance of pathogenic bacteria at the fungal and bacterial levels in normal soil and diseased soil is detected to determine the dosage of the microbial agent; in mid-May, the microbial agent is applied to the diseased soil; the dosage of the microbial agent is determined as follows: When the relative abundance is less than 5%, the dosage of microbial agents is 1.8-2.2 kg / mu. When the relative abundance is 5-10%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 10%, the dosage of microbial agents is 4.5-5.5 kg / mu.

2. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 1, characterized in that: The method for improving diseased soil by testing bacteria and applying fertilizers also includes: (3) In early June of the following year, test the organic matter content in the diseased soil and the relative abundance of pathogenic bacteria at the fungal and bacterial levels in normal soil and diseased soil to determine the dosage of soil conditioners and microbial agents; apply the soil conditioners and microbial agents to the diseased soil in mid-June; The dosage of the microbial agent is determined as follows: When the relative abundance is less than 5%, there is no need to apply microbial agents. When the relative abundance is 10-20%, the dosage of microbial agent is 1.8-2.2 kg / mu. When the relative abundance is 20-30%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 30%, the dosage of microbial agents is 4.5-5.5 kg / mu.

3. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 2, characterized in that: The method for improving diseased soil by testing bacteria and applying fertilizers also includes: (4) In early July of the following year, the relative abundance of pathogenic bacteria at the fungal and bacterial levels in normal soil and diseased soil is detected to determine the dosage of the microbial agent; in mid-July, the microbial agent is applied to the diseased soil; the dosage of the microbial agent is determined as follows: When the relative abundance is less than 10%, there is no need to apply microbial agents. When the relative abundance is 10-20%, the dosage of microbial agent is 1.8-2.2 kg / mu. When the relative abundance is 20-30%, the dosage of microbial agent is 2.5-3.5kg / mu. When the relative abundance is greater than 30%, the dosage of microbial agent is 4.5-5.5kg / mu.

4. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 1, characterized in that: In step (1), the amount of the soil conditioner is determined as follows: When organic matter is less than 15g / kg, soil conditioner is 600kg / mu. When organic matter is 16-25g / kg and soil conditioner is 400kg / mu, When organic matter is 26-35g / kg and soil conditioner is 320kg / mu, When organic matter is greater than 36g / kg, soil conditioner is 200kg / mu.

5. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 1, characterized in that: In step (3), the amount of the soil conditioner is determined as follows: When organic matter is less than 15g / kg, soil conditioner is 200kg / mu. When organic matter is 16-25g / kg and soil conditioner is 160kg / mu, When organic matter is 26-35g / kg and soil conditioner is 120kg / mu, When organic matter is greater than 36g / kg, soil conditioner is 80kg / mu.

6. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to any one of claims 1 to 5, characterized in that: The microbial agent contains Bacillus Velez, Bacillus atrophaeus and Trichoderma, and the number of viable bacteria is ≥ 500 million CFU / g; the method of using the microbial agent is drip irrigation or flushing.

7. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 6, characterized in that: In the microbial agent, the ratio of the number of live bacteria of Bacillus Velez, Bacillus atrophaeus and Trichoderma is 2:(0.3-1):(1-1.8).

8. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 2, characterized in that: The method for applying the soil conditioner in step (1) is as follows: the soil conditioner is evenly spread on the soil surface, and then rotary tillage is performed, and the depth of rotary tillage is 25-50 cm; The method for applying the soil conditioner in step (3) is to evenly spread the soil conditioner on the soil surface and then cover the soil.

9. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 8, characterized in that: The soil conditioner has a live bacterial count of ≥200 million CFU / g, an organic matter content of ≥475%, a total biological humic acid of ≥30%, an easily oxidizable organic matter of ≥20%, and calcium+magnesium+iron+manganese+zinc+iodine of ≥400 mg / kg.

10. The method for repairing and improving diseased soil by testing bacteria and fertilizing according to claim 8, characterized in that: The diseased soil is diseased soil caused by continuous cropping of ginger.

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