Laboratory plant hydroponic device for collecting root system secreted ions and collecting method

Abstract

The invention provides a laboratory plant hydroponic device for collecting root system secreted ions and a collecting method, the laboratory plant hydroponic device for collecting root system secreted ions comprises a box body, the box body is provided with an incubator, the incubator comprises an inner box and an outer box, and the bottom of the inner box is provided with a root system secreted ion collecting device; the invention provides a collecting method for collecting root system secreted ions, which comprises the following steps: on the basis of a laboratory plant hydroponic device for collecting the root system secreted ions, part of plant root systems enter a root system secreted ion collecting device, and plant root system secretions are collected from the root system secreted ion collecting device; adding a buffer solution and a methanol solvent into a root exudate collecting tank, eluting to obtain a plant root exudate ion solution, and freeze-drying to obtain root exudate ions. By utilizing the device and the method provided by the invention, the ions secreted by the root system can be efficiently collected, and the culture box arranged in the box body can realize a repeated control group and a treatment group required by an experiment.

Description

Technical Field

[0001] The invention relates to the technical field of indoor plant hydroponic culture, and in particular to a laboratory plant hydroponic culture device for collecting root secretion ions and a collection method. Background Art

[0002] Plant hydroponics is a new type of soilless plant cultivation method, which is to cultivate plants with water or nutrient solution. The technical principle is to directly immerse the roots of plants in water or nutrient solution, use nutrient solution instead of soil, provide plants with water, nutrients, oxygen and other growth factors, so that plants can grow normally. Compared with traditional soil cultivation, it has the advantages of convenient planting and easy root observation.

[0003] Compared with traditional soil culture, hydroponics has the advantages of being easy to plant and cultivate, high nutrient absorption efficiency, and fast plant growth rate. Laboratory hydroponics takes advantage of the fact that plants can grow normally in nutrient solution, making the hydroponic system conducive to observing plant phenotypes and processing plant materials. It can also facilitate the processing of experimental materials and prevent the influence of external factors on experimental materials.

[0004] The existing laboratory hydroponic device directly fixes the plants in the planting basket, which is not convenient to operate during use. When the taken out materials are put back into the device, the plant materials may be damaged due to the excessive growth of the plant roots and branches and leaves, which is not conducive to the extraction of plant root secretions, and it is time-consuming and labor-intensive during cultivation. In addition, if the existing hydroponic device needs to ensure the good growth of the plants, the water must be changed regularly. The fallen leaves or rotten roots affect the water quality and affect the research of the plant root secretions. The traditional method of collecting root secretion ions requires collecting a large amount of solution for freeze-drying. The process is time-consuming and labor-intensive. The leaf or root loss caused by changing the water and the growth of mold affect the composition of the root secretion ions, affecting the experiment. There are certain defects and deficiencies, so it needs to be improved. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a laboratory plant hydroponic device and a collection method for collecting root secretion ions, thereby effectively solving the deficiencies existing in the prior art.

[0006] In order to solve the above technical problems, an embodiment of the present invention provides a laboratory plant hydroponic device for collecting root secretion ions, comprising a box body, a water supply mechanism is arranged in the box body, a lighting mechanism is arranged at the top of the box body, the lighting mechanism comprises a lamp arranged at the top of the box body, the water supply mechanism comprises a culture platform and a water supply pipeline formed by a fence, a culture box is arranged on the culture platform, the water supply pipeline extends upward along the fence and is connected to the culture box, a water source connection port is arranged on one side of the box body, the water source connection port is connected to the water supply pipeline, and water is supplied to each culture box through the water supply pipeline by an external water pump;

[0007] The incubator comprises an inner box and an outer box, wherein the inner box is nested in the outer box, support frames are arranged on both sides of the inner box, a sliding groove slidably arranged with the outer box is arranged below the support frames, the inner box is covered with a cover plate, the cover plate comprises a movable cover plate and a fixed cover plate, the movable cover plate is mounted on the inner box through the cover plate fixing grooves arranged on both sides of the inner box, the movable cover plate and the fixed cover plate are correspondingly provided with planting basket placement openings, the fixed cover plate is provided with a plurality of planting basket mounting holes corresponding to the planting basket placement openings, the planting basket is fixed to the cover plate through the planting basket placement openings and the planting basket mounting holes, the planting basket is fixed to the cover plate through the planting basket placement openings and the planting basket mounting holes, the planting basket hook cooperates with the movable cover plate to conveniently take and place experimental materials, and avoid the separation of the plant and the planting basket due to the excessively long root system or luxuriant branches and leaves of the plant material; an inner box water supply port is arranged on one side of the top of the inner box, a drainage hole is arranged at the bottom of the inner box, and a root secretion ion collection device is arranged at the bottom of the drainage hole;

[0008] A partition is vertically arranged in the outer box, and a water supply pipe is horizontally arranged on one side of the baffle, the water supply pipe divides the outer box space into two spaces of the size of the inner box, a wireless water pump is arranged at the bottom of the outer box, the water outlet of the wireless water pump is connected to a water pump pipe, the water pump pipe is connected to the water supply pipe along one side of the partition, and the water supply pipe is connected to the water supply port of the inner box.

[0009] The root secretion ion collecting device comprises a root secretion ion collecting groove with an opening at the top, the opening of the root secretion ion collecting groove is arranged corresponding to the drainage hole, and ion exchange resin is arranged in the root secretion ion collecting groove.

[0010] Wherein, a nylon mesh filter membrane is arranged on the drainage hole.

[0011] Among them, the movable cover plate includes a movable cover plate rotating shaft and a movable cover plate rotary vane plate. The two sides of the movable cover plate rotating shaft are rotatably connected to the movable cover plate rotary vane plate. The two ends of the movable cover plate rotating shaft are provided with movable cover plate handles. The movable cover plate rotary vane plate is spliced ​​with the fixed cover plate to form a planting basket placement opening.

[0012] Wherein, a perspective window with scale lines is arranged on the side wall of the outer box, and the water level in the box can be adjusted according to the scale lines on the perspective window of the outer box, so as to control the volume of the liquid in the box and expand the experimental range.

[0013] Wherein, an outer box handle is also provided on one side of the outer box.

[0014] Wherein, a drain port is arranged at the bottom of the outer box, and a drain port valve is fixedly embedded on the drain port.

[0015] Wherein, ventilation holes are opened on both sides of the box body, and a baffle is arranged on the front side of the box body.

[0016] Based on a laboratory plant hydroponic device for collecting root secretion ions, an embodiment of the present invention also provides a method for collecting root secretion ions, comprising the following steps:

[0017] The root secretion ion collecting tank in the laboratory plant hydroponic device for collecting root secretion ions is filled with ion exchange resin;

[0018] Plants are hydroponically cultivated in the inner box, and plant roots grow. Part of the plant roots enter the root secretion ion collecting groove through the drainage hole and the top opening of the root secretion ion collecting groove, so that plant root secretions can be collected from the root secretion collecting groove;

[0019] Add 50-80 ml of methanol solvent into the root secretion collection tank, soak for 5-10 minutes and then collect the methanol solution. Repeat this process 3-5 times to obtain the plant root secretion ion solution, which can be freeze-dried to obtain the root secretion ions.

[0020] The beneficial effects of the above technical solution of the present invention are:

[0021] The present invention can filter out impurities such as roots and leaves that fall off in the hydroponic box through the wireless water pump and the filtering nylon net on the drainage hole, and the ion exchange resin can absorb the anions and cations secreted by the roots in the hydroponic solution, purify the water, retain it and circulate it, and maintain the water quality; the wireless water pump can circulate the water in the outer box to increase the dissolved oxygen in the water and promote the growth of the plants, and the ion exchange resin can be taken out after a unit time and eluted with solvents such as buffer solution and methanol to quickly and efficiently obtain the ion solution secreted by the plant roots, and the root secretion ions can be obtained after freeze drying; at the same time, through the water supply mechanism and the lighting mechanism arranged in the box, the lighting conditions and the hydroponic temperature and other parameters required for the experiment can be controlled, which is convenient for operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the structure of the laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0023] Figure 2 This is a schematic diagram of the structure of a box in a laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0024] Figure 3 This is a schematic diagram of the structure of the outer box in the laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0025] Figure 4 Schematic diagram of the structure of the inner box in the laboratory plant hydroponic device for collecting root secretion ions in the present invention Figure 1 ;

[0026] Figure 5 Schematic diagram of the structure of the inner box in the laboratory plant hydroponic device for collecting root secretion ions in the present invention Figure 2 ;

[0027] Figure 6 It is a schematic diagram of the structure of the planting basket in the laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0028] Figure 7 It is a schematic diagram of the structure of the movable cover plate in the laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0029] Figure 8 It is a schematic diagram of the assembly of the root secretion ion collecting device in the laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0030] Fig. 9 It is a schematic structural diagram of a root secretion ion collecting device in a laboratory plant hydroponic device for collecting root secretion ions in the present invention;

[0031] Fig.10 This is a comparison diagram of the growth phenotypes of Salix matsudana in the experimental group and the control group in Example 1, wherein the plants on the left in the figure are the experimental group and the plants on the right are the control group;

[0032] Fig.11 The growth index statistics of the experimental group and the control group in Example 1 are shown in FIG.

[0033] Fig.12 This is a statistical chart of the root secretion ion content collected from the experimental group and the control group in Example 1.

[0034] Description of reference numerals:

[0035] 1. Box body; 11. Baffle; 12. Ventilation hole; 13. Control panel; 14. Water supply mechanism; 142. Water supply pipeline; 15. Power connection socket;

[0036] 2. Outer box; 21. Wireless water pump; 211. Water pump pipe; 212. Water delivery pipe; 22. Water outlet; 23. Perspective window; 24. Outer box handle; 25. Water storage tank;

[0037] 3. Inner box; 31. Moving cover; 312. Moving cover handle; 313. Moving cover rotary blade; 32. Planting basket; 321. Planting basket socket; 322. Custom basket hook; 33. Support frame; 34. Inner box water supply port; 35. Drain hole; 36. Root secretion ion collection device; 361. Ion exchange resin; 362. Root secretion ion collection tank; 37. Fixed cover. DETAILED DESCRIPTION

[0038] In order to make the technical problems, technical solutions and advantages to be solved by the present invention more clear, a detailed description will be given below with reference to the accompanying drawings and specific embodiments.

[0039] like Figure 1-10 As shown, an embodiment of the present invention provides a laboratory plant hydroponic device for collecting root secretion ions, including a box 1, a water supply mechanism 14 is arranged in the box 1, and an illumination mechanism is arranged at the top of the box 1, and the illumination mechanism includes a lamp arranged at the top of the box (the lamp adopts Signify Lighting (Shanghai) Co., Ltd., model: BN058C), the water supply mechanism 14 includes a culture platform and a water supply pipeline 142 formed by a fence, and a culture box is arranged on the culture platform, and the water supply pipeline 142 extends upward along the fence to connect to the culture box, and a water source connection port is arranged on one side of the box 1, and the water source connection port is connected to the water supply pipeline 142, and water is supplied to each culture box through the water supply pipeline 142 by an external water pump. In this embodiment, ventilation holes 12 are opened on both sides of the box 1, and a baffle 11 is arranged on the front side of the box 1. In this embodiment, three culture platforms and three culture boxes are arranged in the box, and the water supply system and the illumination system are used to realize the three repeated control groups and treatment groups required for the experiment.

[0040] The box 1 is also provided with a control mechanism, which includes a control panel 13 arranged outside the box. The control panel 13 is connected to the lighting mechanism, so that the box has the function of controlling experimental parameters such as lighting and hydroponic temperature.

[0041] The incubator comprises an inner box 3 and an outer box 2, wherein the inner box 3 is nested in the outer box 2. Figure 4 , Figure 5 As shown, support frames 33 are arranged on both sides of the inner box 3, and a sliding groove slidably arranged with the outer box 2 is arranged below the support frame 33, and the inner box 3 is covered with a cover plate, and the cover plate includes a movable cover plate 31 and a fixed cover plate 37, and the movable cover plate 31 is mounted on the inner box 3 through the cover plate fixing grooves 311 arranged on both sides of the inner box 3, and the movable cover plate 31 and the fixed cover plate 37 are correspondingly provided with planting basket placement openings, and the fixed cover plate 37 is provided with a plurality of planting basket mounting holes 321 corresponding to the planting basket placement openings, and the planting basket 32 ​​is fixed to the cover plate through the planting basket placement opening and the planting basket mounting holes 321, and the planting basket hook 322 cooperates with the movable cover plate 31 to conveniently take and place experimental materials, so as to avoid the separation of the plant and the planting basket due to the excessively long root system or lush branches and leaves of the plant material ( Figure 6 ).

[0042] An inner box water inlet 34 is provided on one side of the top of the inner box 3, a drainage hole 35 is provided at the bottom of the inner box 3, a nylon mesh filter membrane is provided on the drainage hole 35, and the nylon mesh can filter out impurities such as roots and leaves that fall off in the hydroponic box, and a root secretion ion collection device 36 is provided at the bottom of the drainage hole 35, and the root secretion ion collection device 36 is inserted below the drainage hole 35 (the insertion assembly method is as follows Figure 8 shown).

[0043] like Fig. 9 As shown, the root secretion ion collection device 36 includes a root secretion ion collection slot 362 with an opening at the top, the opening of the root secretion ion collection slot 362 is arranged corresponding to the drainage hole 35, and an ion exchange resin 361 is arranged in the root secretion ion collection slot 362. The ion exchange resin 361 can absorb the anions and cations secreted by the roots in the hydroponic solution, purify the water, retain and circulate it, and maintain the water quality. In this embodiment, the ion exchange resin is an anion / cation exchange resin, wherein the anion exchange resin has a CAS number of 60177-39-1 and the cation exchange resin has a CAS number of 9002-29-3.

[0044] like Figure 7 As shown, the movable cover 31 includes a movable cover rotating shaft and a movable cover vane plate 313. The two sides of the movable cover rotating shaft are rotatably connected to the movable cover vane plate 313. The two ends of the movable cover rotating shaft are provided with movable cover handles 312. The movable cover vane plate 313 is spliced ​​with the fixed cover 37 to form a planting basket placement opening. The movable cover handle 312 is lifted to retract the movable cover vane plate 313, thereby facilitating the placement of the planting basket.

[0045] like Figure 3 As shown, a partition is vertically arranged in the outer box 2, and a water supply pipe 212 is horizontally arranged on one side of the baffle. The water supply pipe 212 divides the space of the outer box 2 into two spaces of the size of the inner box 3. The bottom of the outer box 2 is a water storage tank 25, which is connected to the root secretion ion collection device 36. A wireless water pump 21 is arranged in the water storage tank 25. The water outlet of the wireless water pump 21 is connected to a water pump pipe 211. The water pump pipe 211 is connected to the water supply pipe 212 along one side of the partition, and the water supply pipe 212 is connected to the water supply port 34 of the inner box. The wireless water pump 21 circulates the water in the outer box through the water pump pipe 211 and the water supply pipe 212, increases the dissolved oxygen in the water, and promotes the growth of the plant. A drain port 22 is arranged at the bottom of the outer box 2, and a drain port valve is fixedly embedded on the drain port 22. (The wireless water pump adopts Jingnuo Aquarium Equipment Co., Ltd., model: JN-201).

[0046] A perspective window 23 with scale lines is provided on the side wall of the outer box 2, and the water level in the box can be adjusted according to the scale lines on the perspective window 23, so as to control the volume of the liquid in the box and expand the experimental range.

[0047] In this embodiment, an outer box handle 24 is further provided on one side of the outer box 2 .

[0048] Based on the above laboratory plant hydroponic device for collecting root secretion ions, an embodiment of the present invention further provides a method for collecting root secretion ions, comprising the following steps:

[0049] The root secretion ion collecting tank in the laboratory plant hydroponic device for collecting root secretion ions is filled with ion exchange resin;

[0050] Plants are hydroponically cultivated in the inner box, and plant roots grow. Part of the plant roots enter the root secretion ion collecting groove through the drainage hole and the top opening of the root secretion ion collecting groove, so that plant root secretions can be collected from the root secretion collecting groove;

[0051] Add 50-80 ml of methanol solvent into the root secretion collection tank, soak for 5-10 minutes and then collect the methanol solution. Repeat this process 3-5 times to obtain the plant root secretion ion solution, which can be freeze-dried to obtain the root secretion ions.

[0052] Next, a specific embodiment is used to illustrate the collection effect of the device of the present invention.

[0053] Embodiment 1

[0054] The plant of Salix matsudana was used as the test material. The 3-5mm thick branches of the current year were cut into 10cm long cuttings. The cuttings were hydroponically cultivated using the device (experimental group). The material placed in a 1.5L plastic hydroponic box for hydroponics was used as the control group. There were 10 treatments in each of the experimental group and the control group, and 1L of sterile water was added to each treatment. The growth conditions were all 12h of sunlight / darkness, 30001x of light intensity, and 25℃±1℃ of temperature, and the total cultivation was 30 days. The plants in the experimental group and the control group were taken out on the 30th day, and their growth status was compared. The growth status was as follows: Fig.10 In addition, it was found that the device of the present invention can significantly promote the growth of Salix matsudana. Further comparison of plant height, root length, dry weight and fresh weight showed that the device of the present invention can significantly improve the growth indicators of the plant, such as plant height, root length, dry weight and fresh weight, and achieve efficient growth of the plant ( Fig.11 ).

[0055] Take out the root secretion ion collection device in the device of the present invention, pour in 50ml of methanol and soak for 10min, collect the methanol solution, repeat this 4 times, and vacuum freeze-dry all the collected methanol solutions in a laboratory basic freeze dryer (Kep, L3-65). After 10h, it can be dried to constant weight and weighed. The control group adopts the traditional method: collect all 1L of root hydroponic solution, and then vacuum freeze-dry it in a laboratory basic freeze dryer (Kep, L3-65). It can be dried to constant weight after 5 days, weighed and compared with the experimental group, and it was found that the total amount of root secretion ions collected by the experimental group did not reach a significant difference compared with the control group ( Fig.12 ), but its operation and collection time are significantly shorter than those of the control group. In summary, the device and method can be used to efficiently collect root secretion ions. At the same time, the device of the present invention can adjust the growth conditions required for hydroponics by controlling the lighting mechanism and the water supply mechanism, and can also significantly improve the plant height, root length, dry weight, fresh weight and other growth indicators, thereby achieving efficient plant growth.

[0056] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A laboratory plant hydroponic device for collecting root secretion ions, characterized in that: It comprises a box body, a water supply mechanism is arranged in the box body, a lighting mechanism is arranged at the top of the box body, the lighting mechanism comprises a lamp arranged at the top of the box body, the water supply mechanism comprises a culture platform and a water supply pipeline formed by a fence, a culture box is arranged on the culture platform, and the water supply pipeline extends upward along the fence to be connected to the culture box; The incubator comprises an inner box and an outer box, wherein the inner box is nested in the outer box, support frames are arranged on both sides of the inner box, a sliding groove slidably arranged with the outer box is arranged below the support frames, the inner box is covered with a cover plate, the cover plate comprises a movable cover plate and a fixed cover plate, the movable cover plate is mounted on the inner box through the cover plate fixing grooves arranged on both sides of the inner box, the movable cover plate and the fixed cover plate are correspondingly provided with planting basket placement openings, the fixed cover plate is provided with a plurality of planting basket mounting holes corresponding to the planting basket placement openings, the planting basket is fixed to the cover plate through the planting basket placement openings and the planting basket mounting holes, an inner box water supply port is arranged on one side of the top of the inner box, a drainage hole is arranged at the bottom of the inner box, and a root secretion ion collection device is arranged at the bottom of the drainage hole; A partition is vertically arranged in the outer box, and a water supply pipe is horizontally arranged on one side of the baffle. A wireless water pump is arranged at the bottom of the outer box, and the water outlet of the wireless water pump is connected to the water pump pipe. The water pump pipe is connected to the water supply pipe along one side of the partition, and the water supply pipe is connected to the water supply port of the inner box.

2. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: The root secretion ion collecting device comprises a root secretion ion collecting groove with an opening at the top, the opening of the root secretion ion collecting groove is arranged corresponding to the drainage hole, and ion exchange resin is arranged in the root secretion ion collecting groove.

3. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: A nylon mesh filter membrane is arranged on the drainage hole.

4. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: The movable cover plate includes a movable cover plate rotating shaft and a movable cover plate rotary vane plate. Both sides of the movable cover plate rotating shaft are rotatably connected to the movable cover plate rotary vane plate. Both ends of the movable cover plate rotating shaft are provided with movable cover plate handles. The movable cover plate rotary vane plate is spliced ​​with the fixed cover plate to form a planting basket placement opening.

5. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: A perspective window with scale lines is arranged on the side wall of the outer box.

6. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: An outer box handle is also provided on one side of the outer box.

7. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: A water outlet is arranged at the bottom of the outer box, and a water outlet valve is fixedly embedded on the water outlet.

8. The laboratory plant hydroponic device for collecting root secretion ions according to claim 1, characterized in that: Ventilation holes are provided on both sides of the box body, and a baffle is provided on the front side of the box body.

9. A method for collecting root secretion ions, characterized in that: The steps include: The root secretion ion collecting tank in the laboratory plant hydroponic device for collecting root secretion ions according to any one of claims 1 to 8 is filled with ion exchange resin; Plants are hydroponically cultivated in the inner box, and plant roots grow. Part of the plant roots enter the root secretion ion collecting groove through the drainage hole and the top opening of the root secretion ion collecting groove, so that plant root secretions can be collected from the root secretion collecting groove; Add 50-80 ml of methanol solvent into the root secretion collection tank, soak for 5-10 minutes and then collect the methanol solution. Repeat this process 3-5 times to obtain the plant root secretion ion solution, which can be freeze-dried to obtain the root secretion ions.

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