Automatic layered loading device for centrifuge
By designing an automatic layering loading device for centrifuge, the problem of liquid disturbance during secondary loading in liquid layering experiments is solved, and the stability and accuracy of liquid layering are achieved.
Patent Information
- Application Number
- CN202411579936.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-07
- Publication Date
- 2025-02-07
- Estimated Expiration
- 2044-11-07
AI Technical Summary
In liquid stratification experiments, the liquid disturbance caused by the centrifuge stop, manual operation or rapid braking during secondary loading, resulting in less obvious layer of liquid, making it difficult to accurately extract specific layers of liquid.
An automatic layered loading device for centrifuge is designed, including multiple placement mechanisms, control mechanisms and loading mechanisms. The initial layer of liquid is realized through centrifugal force, and a specific layer of liquid is extracted by a control mechanism, and then a second time is performed through the loading mechanism. Load to keep the liquid layered and stable.
It realizes automatic extraction of any layer of liquid after layering in the centrifuge state, avoids liquid mixing, ensures the stability of layering, and facilitates liquid layering experiments.
Smart Images

Figure CN119076240B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of centrifuge stratification experiments, in particular to an automatic stratification loading device for a centrifuge. Background Art
[0002] The process of putting a sample into a centrifuge tube and placing it in a rotor for centrifugation is called loading. In the liquid stratification experiment, the original solution mixed with different substances is added to the test tube, and then the test tube is placed in the rotor of the centrifuge for loading. The liquid inside the test tube will be stratified due to centrifugal force. This is because the densities of different substances in the liquid are different. Under the influence of centrifugal force, the substances with higher density in the liquid will move away from the centrifuge rotor, and the substances with lower density will move closer to the centrifuge rotor, so that the original solution will be stratified, and the substances in each layer of the liquid after stratification are different;
[0003] However, in the process of liquid stratification experiment using a centrifuge, if a certain layer of liquid in the liquid after the original liquid is stratified needs to be extracted separately for secondary loading and stratification, such as: after the blood is initially stratified by a centrifuge, a plasma layer, a white blood cell and platelet layer, and a red blood cell layer can be formed, and the plasma layer can be stratified for secondary stratification to separate proteins, metabolites, hormones, enzymes, drugs and poisons in the plasma. In medicine, the drug concentration and poison level in the plasma can be measured for drug monitoring and poisoning diagnosis, and pathogens can also be stratified from the plasma layer for medical identification;
[0004] In specific operations, after the initial loading and stratification of the original liquid, it is generally necessary to wait until the centrifuge stops rotating, take out the test tube, and then use a tool to extract a layer of liquid after stratification, and then put it into a new test tube for secondary loading. In this process, the stratified liquid in the test tube may produce liquid disturbance due to various reasons, resulting in unclear liquid stratification and mixing. For example: when the centrifuge stops, the liquid flow is disturbed due to liquid inertia, resulting in unclear stratification, or the vibration generated when the test tube is manually picked up causes disturbance of the stratified liquid, resulting in unclear stratification, or the centrifuge braking process is fast, causing the liquid in the test tube to be disturbed by impact, thereby affecting the unclear liquid stratification, or when the centrifuge is taken out, the human body temperature affects the temperature of the liquid in the test tube, resulting in unclear stratification. Various reasons will cause the user to extract liquid and cause mixing during the secondary loading due to the unclear stratification of the original liquid, which is inconvenient for the secondary loading and stratification experiment of the stratified liquid. Summary of the invention
[0005] The object of the present invention is to provide an automatic layered loading device for a centrifuge to solve the problems raised in the above background technology.
[0006] To achieve the above object, the present invention provides the following technical solutions:
[0007] An automatic layered loading device for a centrifuge, comprising:
[0008] A machine body, a shell, multiple placement mechanisms for primary loading, a control mechanism for extracting layered materials from the multiple placement mechanisms, and a loading mechanism for secondary loading. A hollow cylinder is fixedly connected to the top of the rotor inside the machine body, a ring body is fixedly connected to the center of the top surface of the hollow cylinder, and a plurality of insertion ports are provided on the outer wall of the ring body. The bottom surface of the shell is fixedly connected to the top surface of the machine body, a sleeve hole is provided at the center of the inner bottom surface of the shell, and the bottom end of the outer wall of the hollow cylinder is rotatably sleeved with the inner wall of the sleeve hole. Multiple placement mechanisms correspond to the plurality of insertion ports one by one. The placement mechanism includes a test tube, and a thread is provided on the inner side of the test tube opening. A rubber ring is screwed on one end of the inner wall of the test tube, and an insertion tube is slidably sleeved inside the rubber ring. The control mechanism is located above the ring body, and the loading mechanism is located at the outer wall of the hollow cylinder.
[0009] Further, the control mechanism comprises:
[0010] Multiple L-shaped frames, hollow shells and first bearings, multiple L-shaped frames are all located inside the ring body, multiple short arms of the L-shaped frames are fixedly connected to the top surface of the hollow cylinder at one end, and multiple long arms of the L-shaped frames are provided with card slots on the top surface, multiple L-shaped frames correspond one-to-one to multiple pipe insertion ports, the outer wall of the hollow shell is fixedly connected to one end of the long arms of the L-shaped frames, the bottom surface of the hollow shell is fixedly connected to the top surface of the hollow cylinder, the first bearing is located above the ring body, the bottom surface of the inner ring of the first bearing is rotatably connected to multiple support arms, and the bottom ends of the multiple support arms are rotatably connected to sliders, the multiple sliders correspond one-to-one to the multiple card slots, and the bottom ends of the multiple sliders are slidably connected to the corresponding card slots, and one side of the multiple sliders is provided with fixing holes, and the outer wall of any insertion tube is fixedly sleeved with the inner wall of the adjacent fixing hole.
[0011] Furthermore, two installation openings are provided on the top surface of the shell, and a fixing cylinder is fixedly connected to the center of the inner top surface of the shell, two electric push rods are fixedly connected to the bottom surface of the fixing cylinder, and the movable ends of the two electric push rods are fixedly connected to the top surface of the outer ring of the first bearing.
[0012] Further, the loading mechanism comprises:
[0013] There are multiple guide rails and multiple clamping rods, and the multiple guide rails correspond one-to-one with the multiple tube insertion ports. One ends of the multiple guide rails are fixedly connected to the top of the outer wall of the hollow tube. Multiple clamping rods correspond one-to-one with the multiple guide rails. The multiple clamping rods are slidably clamped in the corresponding guide rails, and the outer walls of the multiple clamping rods are fixedly connected with vacuum tubes, and one ends of the openings of the multiple vacuum tubes are fixedly connected with hoses.
[0014] Furthermore, a collar is slidably sleeved on the outer wall of the hollow cylinder, and a plurality of support plates are rotatably connected to the outer wall of the collar, a plurality of support plates are rotatably connected to a U-shaped block at one end, and a plurality of U-shaped blocks correspond to a plurality of clamping rods one by one, a plurality of guide rails are provided with rectangular holes on opposite sides, and one end of two arms of a plurality of U-shaped blocks are respectively fixedly connected to two ends of corresponding clamping rods, a second bearing is provided on the bottom surface of the collar, and a top surface of an inner ring of the second bearing is fixedly connected to the bottom surface of the collar, two hydraulic push rods are fixedly connected to the inner bottom surface of the shell, and the movable ends of the two hydraulic push rods are fixedly connected to the outer wall of the outer ring of the second bearing through a connecting frame.
[0015] Further, multiple long sides of the top surfaces of the guide rails are provided with L-shaped plates, and the short arms of any L-shaped plates are fixedly connected to the top surfaces of adjacent guide rails, a third bearing is provided above the ring body, and a plurality of movable grooves are provided on the bottom surface of the inner ring of the bearing of the third bearing, a movable plate is provided above the guide rails, the plurality of movable grooves correspond to the plurality of movable plates one by one, and the top ends of the plurality of movable plates are slidably engaged in the corresponding movable grooves, two sliding rods are provided below the plurality of movable plates, any sliding rod is slidably engaged between the two adjacent short arms of the L-shaped plates, the outer side wall of any sliding rod is fixedly connected to two support rods, and the two support rods on any sliding rod are rotatably connected to the bottom ends of the adjacent movable plates, the plurality of movable plates are equipped with rotating rings, and a circular through hole is provided on one side of the plurality of rotating rings, the plurality of circular through holes are rotatably sleeved with the outer side wall of an adjacent sliding rod, and the inner side walls of the plurality of rotating rings are slidably sleeved with the outer side walls of adjacent vacuum tubes.
[0016] Furthermore, two hydraulic cylinders are fixedly connected to the inner top surface of the housing, and the movable ends of the two hydraulic cylinders are fixedly connected to the top surface of the outer ring of the third bearing.
[0017] Furthermore, multiple top surfaces of the hollow shells are provided with L-shaped grooves, and one ends of the multiple L-shaped grooves are located at the outer wall of the hollow shells, the multiple L-shaped grooves correspond to the multiple hoses one by one, and the interiors of the multiple L-shaped grooves are connected to the interior of the hollow shells, one ends of the multiple hoses pass through the corresponding L-shaped grooves, and one ends of the multiple hoses are fixedly connected to one end of the inner wall of the adjacent insertion tube.
[0018] Furthermore, a connecting tube is fixedly connected to the center of the top surface of the hollow shell, and the bottom end of the connecting tube penetrates the inner top surface of the hollow shell and is fixedly connected to an airbag, the airbag is in contact with multiple hoses, and an air pump is matched with the top surface of the shell, and the air pump is fixedly connected to a catheter, which is rotatably connected to the top end of the connecting tube.
[0019] Furthermore, a plurality of air inlets are provided at one end of each of the plurality of insertion tubes.
[0020] Compared with the prior art, the present invention has the following beneficial effects:
[0021] By placing the original liquid into the placement mechanism, and selecting a suitable number of placement mechanisms to insert into the insertion port, and then starting the machine body to drive the hollow cylinder and the ring body to rotate, the original liquid inside the placement mechanism is initially stratified due to centrifugal force, and then the control mechanism can be used to cooperate with the loading mechanism to suck any layer of liquid after stratification inside the placement mechanism, and then the position and angle of the loading mechanism are controlled to make the sucked liquid inside the loading mechanism stratified again due to centrifugal force, and the centrifuge is not stopped during the process, so that the liquid stratification in the placement mechanism remains stable, avoiding liquid mixing, and facilitating liquid stratification experiments.
[0022] The method is to put the original liquid into the test tube, and tighten and block the opening of the test tube with a rubber ring, then insert the test tube into the insertion port, connect the adjacent hose to the insertion tube, and bond the insertion tube to the adjacent slider, then start the air pump to expand the airbag to squeeze and seal the hose, and then start the body to drive the hollow tube and the ring body to rotate, so that the liquid in the test tube is naturally stratified due to centrifugal force, and then start the two electric push rods to drive the first bearing to press down, so that the first bearing synchronously presses multiple arms away from the center of the hollow tube by the same distance, so that the arms drive the insertion tube to extend into the adjacent test tube until the insertion tube moves to any layer of the stratified liquid in the test tube, and then start the air pump to extract air from the airbag to make the airbag shrink and the hose unobstructed, and then the vacuum tube automatically uses the hose to suck the stratified liquid in the test tube.
[0023] After the vacuum tube sucks the layered liquid in the test tube, the two hydraulic push rods can be started to drive the ring to move, so that the ring drives all vacuum tubes to move away from or close to the hollow tube at the same distance synchronously through multiple support plates, thereby adjusting the centrifugal force on the liquid inside the vacuum tube at the same speed of the hollow tube;
[0024] When the vacuum tube is driven to move by the adjacent support plates, it will drive the adjacent moving plates to move synchronously through the connecting ring, and then start the two hydraulic cylinders to drive the third bearing to move, so that the third bearing synchronously drives multiple moving plates to move, so that any moving plate presses the two adjacent sliding bars to move toward or away from each other, and the position of the vacuum tube and the clamping rod is fixed by the adjacent support plates, so that when the two sliding bars under any moving plate move, the adjacent vacuum tubes can be pulled to rotate through the connecting ring, and the inclination angle of the vacuum tube can be controlled, so that the liquid is redirected during the centrifugation process, thereby improving the separation efficiency, and can be adjusted according to different liquids, so that it is convenient for users to use. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;
[0026] Figure 2 It is a schematic diagram of the shell structure in the present invention;
[0027] Figure 3It is a schematic diagram of the positional relationship between the control mechanism and the loading mechanism in the present invention;
[0028] Figure 4 It is a schematic diagram of the positional relationship between the loading mechanism and the hollow cylinder in the present invention;
[0029] Figure 5 It is a schematic diagram of the control mechanism structure in the present invention;
[0030] Figure 6 It is an exploded diagram of the placement mechanism and the control mechanism structure of the present invention;
[0031] Figure 7 It is a schematic diagram of the cross-sectional structure of the hollow shell in the present invention;
[0032] Figure 8 It is an exploded diagram of the loading mechanism structure in the present invention;
[0033] Fig. 9 The present invention Figure 8 A partial enlarged view of the middle part;
[0034] Fig.10 It is a schematic diagram of the structure of the clamping rod, the sliding rod and the rotating ring in the present invention;
[0035] Fig.11 It is a schematic diagram of the cross-sectional structure of the insertion tube in the present invention.
[0036] In the figure: 100, body; 110, hollow cylinder; 120, ring body; 121, intubation port; 200, shell; 201, sleeve hole; 300, placement mechanism; 310, test tube; 320, rubber ring; 330, insertion tube; 331, air inlet; 400, control mechanism; 410, L-shaped frame; 420, hollow shell; 421, L-shaped groove; 430, connecting pipe; 431, air bag; 440, first bearing; 441, support arm; 442 , slider; 450, fixed cylinder; 451, electric push rod; 500, loading mechanism; 510, guide rail; 511, L-shaped plate; 520, clamping rod; 521, vacuum tube; 522, hose; 530, collar; 531, support plate; 532, U-shaped block; 540, second bearing; 541, hydraulic push rod; 550, third bearing; 551, moving groove; 552, hydraulic cylinder; 560, moving plate; 570, slide rod; 571, rotating ring. DETAILED DESCRIPTION
[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0038] See also Figure 1-Figure 11 In an embodiment of the present invention, an automatic layered loading device for a centrifuge includes:
[0039] The machine body 100, the shell 200, a plurality of placement mechanisms 300 for primary loading, a control mechanism 400 for extracting layered materials from the plurality of placement mechanisms 300, and a loading mechanism 500 for secondary loading, the top of the rotor in the machine body 100 is fixedly connected to a hollow cylinder 110, the center of the top surface of the hollow cylinder 110 is fixedly connected to a ring body 120, and the outer wall of the ring body 120 is provided with a plurality of insertion openings 121, the bottom surface of the shell 200 is fixedly connected to the top surface of the machine body 100, and the inner bottom of the shell 200 is fixedly connected to the inner bottom of the shell 200. A sleeve hole 201 is opened in the center of the surface, and the bottom end of the outer wall of the hollow cylinder 110 is rotatably sleeved with the inner wall of the sleeve hole 201. Multiple placement mechanisms 300 correspond to multiple insertion ports 121 one by one. The placement mechanism 300 includes a test tube 310, and a thread is opened on the inner side of the opening of the test tube 310. A rubber ring 320 is screwed on one end of the inner wall of the test tube 310, and an insertion tube 330 is slidably sleeved inside the rubber ring 320. The control mechanism 400 is located above the ring body 120, and the loading mechanism 500 is located at the outer wall of the hollow cylinder 110.
[0040] Specifically, by connecting the hollow cylinder 110 with the rotor inside the body 100, the body 100 can drive the hollow cylinder 110 to rotate at a high speed to generate centrifugal force. When in use, the liquid to be layered (raw liquid) is added into the multiple placement mechanisms 300, and then the placement mechanisms 300 are inserted into the multiple insertion ports 121 for fixation, and then the body 100 is started to drive the hollow cylinder 110 and the ring body 120 to rotate, so that the raw liquid inside the multiple placement mechanisms 300 is layered due to the centrifugal force, and the raw liquid is loaded for the first time, and then the layered liquid in the multiple placement mechanisms 300 is extracted by using the control mechanism 400, and the liquid of any layer after the raw liquid in the placement mechanism 300 is extracted by the control mechanism 400, and the extracted liquid is added to the loading mechanism 500, and the single separation layer liquid in the liquid after the raw liquid is layered is loaded for the second time by using the loading mechanism 500, and the body 100 can always be in operation during the first loading and second loading processes, thereby ensuring The liquid in the holding and placing mechanism 300 is always clearly layered due to the centrifugal force and is not easily mixed. In actual use, the original liquid can be added to the test tube 310. The outer wall of the rubber ring 320 is provided with a thread, which can be screwed into the opening of the test tube 310, and the insertion tube 330 can be inserted into the rubber ring 320. The outer wall of the insertion tube 330 and the inner wall of the rubber ring 320 are both provided with a super-slip coating to reduce friction and prevent the liquid from flowing out from between the rubber ring 320 and the insertion tube 330. Then the test tube 310 is inserted into the insertion port 1 21, the larger diameter of the opening of the test tube 310 will naturally get stuck at the insertion port 121, and then when the body 100 starts the hollow cylinder 110 and the ring body 120 to rotate, the liquid is not easy to leak out from the inside of the test tube 310, and after the original liquid in the test tube 310 is stratified, the insertion tube 330 can be moved to insert the insertion tube 330 into any layer formed after the original liquid is stratified, and then the insertion tube 330 is used to suck the liquid in the layer and transport it to the loading mechanism 500 for secondary loading. Example
[0041] like Figure 2-Figure 6 As shown, in this embodiment, the control mechanism 400 includes:
[0042] A plurality of L-shaped frames 410, a hollow shell 420 and a first bearing 440, wherein the plurality of L-shaped frames 410 are all located inside the ring body 120, one end of the short arms of the plurality of L-shaped frames 410 are all fixedly connected to the top surface of the hollow cylinder 110, and a slot is provided on the top surface of the long arms of the plurality of L-shaped frames 410, and the plurality of L-shaped frames 410 correspond one to one with the plurality of intubation ports 121, the outer wall of the hollow shell 420 is fixedly connected to one end of the long arms of the plurality of L-shaped frames 410, the bottom surface of the hollow shell 420 is fixedly connected to the top surface of the hollow cylinder 110, the first bearing 440 is located above the ring body 120, and the bottom surface of the inner ring of the first bearing 440 is rotatably connected to a plurality of support arms 441 , and the bottom ends of the multiple support arms 441 are rotatably connected with sliders 442, the multiple sliders 442 correspond to the multiple card slots one by one, and the bottom ends of the multiple sliders 442 are slidably connected to the corresponding card slots, and the multiple sliders 442 are provided with fixing holes on one side, and the outer side wall of any insertion tube 330 is fixedly sleeved with the inner side wall of the adjacent fixing hole, the top surface of the shell 200 is provided with two installation openings, and the center of the inner top surface of the shell 200 is fixedly connected with a fixing cylinder 450, and the bottom surface of the fixing cylinder 450 is fixedly connected with two electric push rods 451, and the movable ends of the two electric push rods 451 are fixedly connected to the top surface of the outer ring of the first bearing 440.
[0043] In this embodiment, when inserting the test tube 310 into the insertion port 121, the installation port can be used to facilitate manual operation by the user, and attention should be paid to keeping the center of gravity of the hollow cylinder 110 stable, that is, a suitable number of test tubes 310 are selected and inserted into the insertion port 121 symmetrically with the hollow cylinder 110 as the center. After the test tubes 310 are inserted, the insertion tubes 330 on the test tubes 310 can be fixed to the inside of the fixing holes on the adjacent sliders 442 by glue, and then the ring body 120 is driven to rotate by starting the body 100, so that the original liquid in the test tube 310 is naturally layered due to the centrifugal force, and then the two electric The movable push rod 451 can drive the first bearing 440 to move downward, so that the first bearing 440 synchronously pushes the multiple support arms 441 and the slider 442 away from the center of the hollow cylinder 110, so that the slider 442 drives one end of the adjacent insertion tube 330 to extend into the adjacent test tube 310, until one end of the insertion tube 330 moves to a suitable position to suck any layer of liquid after the original liquid is layered. During the experiment, multiple test tubes 310 can be set to load the same original liquid of the same specification for batch production of experimental samples. The situation inside the test tube 310 can be monitored using a high-speed camera in combination with image processing technology, which are all existing technologies and will not be repeated here.
[0044] like Figure 8-Figure 10 As shown, in this embodiment, the loading mechanism 500 includes:
[0045] A plurality of guide rails 510 and a plurality of clamping rods 520, the plurality of guide rails 510 correspond to the plurality of intubation ports 121 one by one, one end of the plurality of guide rails 510 is fixedly connected to the top of the outer wall of the hollow cylinder 110, the plurality of clamping rods 520 correspond to the plurality of guide rails 510 one by one, the plurality of clamping rods 520 are slidably clamped in the corresponding guide rails 510, and the outer walls of the plurality of clamping rods 520 are fixedly connected to vacuum tubes 521, and one end of the openings of the plurality of vacuum tubes 521 is fixedly connected to hoses 522, the outer wall of the hollow cylinder 110 is slidably sleeved with a collar 530, and the outer wall of the collar 530 is rotatably connected to a plurality of support plates 531, and the plurality of support plates One end of the plate 531 is rotatably connected to a U-shaped block 532, and the multiple U-shaped blocks 532 correspond to the multiple clamping rods 520 one by one. Rectangular holes are opened on the opposite sides of the multiple guide rails 510, and one end of the two arms of the multiple U-shaped blocks 532 are respectively fixedly connected to the two ends of the corresponding clamping rods 520. A second bearing 540 is arranged on the bottom surface of the ring 530, and the top surface of the inner ring of the second bearing 540 is fixedly connected to the bottom surface of the ring 530. Two hydraulic push rods 541 are fixedly connected to the inner bottom surface of the shell 200, and the movable ends of the two hydraulic push rods 541 are fixedly connected to the outer side wall of the outer ring of the second bearing 540 through a connecting frame.
[0046] During specific implementation, the inside of the vacuum tube 521 is evacuated in advance using a vacuum pump or the like, and then the hose 522 is sealed to keep the inside of the vacuum tube 521 in a negative pressure vacuum state at all times. When in use, the hose 522 can be fixed to the inner wall of the insertion tube 330 on the adjacent insertion port 121, and the hose 522 can be pinched to prevent the vacuum tube 521 from sucking the liquid inside the adjacent test tube 310. After one end of the insertion tube 330 is moved to a suitable position inside the adjacent test tube 310, the hose 522 can be loosened to allow the vacuum tube 521 to naturally suck a layer of liquid after stratification in the adjacent test tube 310 due to its own internal negative pressure vacuum state. Then, as the body 100 drives the hollow cylinder 110 to rotate, the hollow cylinder 110 drives multiple vacuum tubes 521 to rotate synchronously, so that the liquid sucked inside the vacuum tube 521 is stratified again due to centrifugal force.
[0047] like Figure 3-Figure 4 and Figure 8-Figure 10As shown, in this embodiment, L-shaped plates 511 are provided on both long sides of the top surfaces of the multiple guide rails 510, and the short arms of any L-shaped plates 511 are fixedly connected to the top surfaces of the adjacent guide rails 510. A third bearing 550 is provided above the ring body 120, and a plurality of movable grooves 551 are provided on the bottom surface of the inner ring of the third bearing 550. A movable plate 560 is provided above the multiple guide rails 510, and the multiple movable grooves 551 correspond to the multiple movable plates 560 one by one, and the top ends of the multiple movable plates 560 are slidably engaged in the corresponding movable grooves 551. Two sliding rods 570 are provided below the multiple movable plates 560, and any sliding rod 570 is slidably engaged in the two adjacent sliding rods. Between the short arms of the L-shaped plates 511, the outer wall of any sliding rod 570 is fixedly connected to two support rods, the two support rods on any sliding rod 570 are rotatably connected to the bottom end of the adjacent movable plate 560, multiple movable plates 560 are equipped with rotating rings 571, and circular through holes are opened on one side of multiple rotating rings 571, multiple circular through holes are rotatably sleeved on the outer wall of an adjacent sliding rod 570, and the inner walls of multiple rotating rings 571 are slidably sleeved on the outer wall of the adjacent vacuum tube 521, two hydraulic cylinders 552 are fixedly connected to the inner top surface of the shell 200, and the movable ends of the two hydraulic cylinders 552 are fixedly connected to the top surface of the outer ring of the third bearing 550.
[0048] In specific implementation, after a certain layer of liquid after the original liquid is stratified is sucked by the adjacent vacuum tube 521, the second bearing 540 and the sleeve ring 530 can be driven to move upward synchronously by starting the two hydraulic push rods 541, so that during the movement of the sleeve ring 530, multiple support plates 531 are pushed to move synchronously away from the hollow cylinder 110 by the same distance, thereby adjusting the distance between the multiple vacuum tubes 521 and the hollow cylinder 110. When the distance is farther from the hollow cylinder 110, the centrifugal force on the liquid inside the vacuum tube 521 is greater, and when the distance is closer to the hollow cylinder 110, the centrifugal force on the liquid inside the vacuum tube 521 is smaller. During the movement of the vacuum tube 521, the adjacent moving plate 560 and the sliding rod 570 can be pulled to move synchronously by the adjacent rotating ring 571, and then the two hydraulic cylinders 55 are started. 2 can drive the third bearing 550 to press down, so that the multiple moving plates 560 move downward synchronously, so that the multiple moving plates 560 synchronously press the two adjacent sliding bars 570 to move away from each other through the adjacent supporting rods, and the position of the clamping rod 520 under any moving plate 560 is limited by the sleeve ring 530 through the adjacent supporting plate 531 and does not change, so that when the two sliding bars 570 under any moving plate 560 move away from each other, the adjacent rotating ring 571 will move away from the adjacent vacuum tube 521 with the movement of the adjacent sliding bar 570, and pull the adjacent vacuum tube 521 to a tilted state, so as to control the tilt angle of the vacuum tube 521, so that the liquid is redirected during the centrifugation process, thereby improving the separation efficiency, and can be adjusted according to different liquids, which is convenient for users. Example
[0049] On the basis of the first embodiment, the opening and closing of the plurality of hoses 522 are controlled by providing an air bag 431 .
[0050] like Figure 6-Figure 7 and Fig.11 As shown, in the present embodiment, L-shaped grooves 421 are provided on the top surfaces of the plurality of hollow shells 420, and one ends of the plurality of L-shaped grooves 421 are located at the outer wall of the hollow shell 420, the plurality of L-shaped grooves 421 correspond one-to-one to the plurality of hoses 522, and the interiors of the plurality of L-shaped grooves 421 are connected to the interior of the hollow shell 420, one ends of the plurality of hoses 522 pass through the interiors of the corresponding L-shaped grooves 421, and one ends of the plurality of hoses 522 are fixedly connected to one end of the inner wall of the adjacent insertion tube 330, a connecting tube 430 is fixedly connected to the center of the top surface of the hollow shell 420, and the bottom end of the connecting tube 430 passes through the inner top surface of the hollow shell 420 and is fixedly connected to an airbag 431, the airbag 431 is in contact with the plurality of hoses 522, an air pump is provided on the top surface of the shell 200, and the air pump is fixedly connected to a conduit, the conduit is rotatably connected to the top end of the connecting tube 430, and a plurality of air inlets 331 are provided on one end of the plurality of insertion tubes 330.
[0051] In a specific implementation, the catheter is a hard tube. When it is used, when it is necessary to close multiple hoses 522, the air pump can be started to inject air into the airbag 431 through the catheter and the connecting tube 430 to expand the airbag 431, so that the expanded airbag 431 presses the multiple hoses 522 so that the multiple hoses 522 are closed synchronously, making it difficult for the vacuum tube 521 to suck the liquid inside the adjacent test tube 310 through the hose 522. When the air pump is started to extract the gas inside the airbag 431, the airbag 431 shrinks, and the multiple hoses 522 are unobstructed synchronously, so that the vacuum tube 521 can suck the liquid inside the adjacent test tube 310 through the hose 522. When the vacuum tube 521 sucks the liquid inside the adjacent test tube 310, the test tube 310 can be aired through the multiple air inlets 331 to avoid negative pressure inside the test tube 310. The amount of raw liquid inside the test tube 310 is small, and it will be concentrated at the bottom of the test tube 310 during the centrifugation process. The air inlet 331 is an L-shaped structure. During the centrifugation process, the air inlet 331 is located outside the test tube 310 as a whole. When the liquid inside the test tube 310 needs to be sucked, the insertion tube 330 is partially inserted into the raw liquid layer in the test tube 310, and the air inlet 331 is at a certain distance from the liquid, so that the liquid is not easy to flow out from the air inlet 331.
[0052] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.
[0053] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.
Claims
1. An automatic layered loading device for a centrifuge, characterized in that: include: A machine body (100), wherein a hollow cylinder (110) is fixedly connected to the top of the internal rotor, a ring body (120) is fixedly connected to the center of the top surface of the hollow cylinder (110), and a plurality of pipe insertion openings (121) are provided on the outer side wall of the ring body (120); The shell (200) has a bottom surface fixedly connected to the top surface of the machine body (100), a sleeve hole (201) is provided at the center of the inner bottom surface of the shell (200), and the bottom end of the outer wall of the hollow cylinder (110) is rotatably sleeved with the inner wall of the sleeve hole (201); A plurality of placement mechanisms (300) corresponding one to one with the plurality of insertion ports (121), the placement mechanisms (300) comprising a test tube (310), the inner side of the opening of the test tube (310) being provided with a thread, a rubber ring (320) being screwed onto one end of the inner side wall of the test tube (310), and an insertion tube (330) being slidably sleeved inside the rubber ring (320); A control mechanism (400) located above the ring body (120); A loading mechanism (500) is located on the outer side wall of the hollow cylinder (110); The control mechanism (400) comprises: A plurality of L-shaped frames (410) are all located inside the ring body (120); one end of the short arms of the plurality of L-shaped frames (410) is fixedly connected to the top surface of the hollow cylinder (110); and a slot is provided on the top surface of the long arms of the plurality of L-shaped frames (410); and the plurality of L-shaped frames (410) correspond one to one to the plurality of intubation ports (121); A hollow shell (420), the outer wall of which is fixedly connected to one end of the long arms of the plurality of L-shaped frames (410), and the bottom surface of the hollow shell (420) is fixedly connected to the top surface of the hollow cylinder (110); A first bearing (440) is located above the ring body (120); the bottom surface of the inner ring of the first bearing (440) is rotatably connected to a plurality of support arms (441); the bottom ends of the plurality of support arms (441) are rotatably connected to sliders (442); the plurality of sliders (442) correspond to the plurality of slots one by one; the bottom ends of the plurality of sliders (442) are slidably engaged in the corresponding slots; a fixing hole is provided on one side of the plurality of sliders (442); and the outer wall of any insertion tube (330) is fixedly sleeved to the inner wall of an adjacent fixing hole; The loading mechanism (500) comprises: A plurality of guide rails (510) corresponding one to one with the plurality of tube insertion ports (121), and one end of each of the plurality of guide rails (510) being fixedly connected to the top end of the outer wall of the hollow cylinder (110); A plurality of clamping rods (520) corresponding one to the plurality of guide rails (510); the plurality of clamping rods (520) are slidably clamped inside the corresponding guide rails (510); the outer side walls of the plurality of clamping rods (520) are fixedly connected to vacuum tubes (521); and one end of the openings of the plurality of vacuum tubes (521) is fixedly connected to a hose (522); The top surfaces of the plurality of hollow shells (420) are each provided with an L-shaped groove (421), and one end of the plurality of L-shaped grooves (421) is located at the outer wall of the hollow shell (420), the plurality of L-shaped grooves (421) correspond to the plurality of hoses (522) one by one, and the interiors of the plurality of L-shaped grooves (421) are connected to the interior of the hollow shell (420), one end of the plurality of hoses (522) passes through the interior of the corresponding L-shaped groove (421), and one end of the plurality of hoses (522) is fixedly connected to one end of the inner wall of the adjacent insertion tube (330); A plurality of air inlets (331) are provided at one end of each of the plurality of insertion tubes (330); A connecting tube (430) is fixedly connected to the center of the top surface of the hollow shell (420), and the bottom end of the connecting tube (430) penetrates the inner top surface of the hollow shell (420) and is fixedly connected to an air bag (431), the air bag (431) is in contact with a plurality of hoses (522), an air pump is matched with the top surface of the shell (200), and the air pump is fixedly connected to a conduit, and the conduit is rotatably connected to the top end of the connecting tube (430); The outer wall of the hollow cylinder (110) is slidably sleeved with a collar (530), and the outer wall of the collar (530) is rotatably connected with a plurality of support plates (531), one end of each of the plurality of support plates (531) is rotatably connected with a U-shaped block (532), and the plurality of U-shaped blocks (532) correspond one to one with the plurality of clamping rods (520), rectangular holes are provided on opposite sides of the plurality of guide rails (510), and one end of both arms of the plurality of U-shaped blocks (532) is respectively fixedly connected to the two ends of the corresponding clamping rods (520), a second bearing (540) is provided on the bottom surface of the collar (530), and the top surface of the inner ring of the second bearing (540) is fixedly connected to the bottom surface of the collar (530), two hydraulic push rods (541) are fixedly connected to the inner bottom surface of the housing (200), and the movable ends of the two hydraulic push rods (541) are fixedly connected to the outer wall of the outer ring of the second bearing (540) through a connecting frame; L-shaped plates (511) are provided on both long sides of the top surfaces of the plurality of guide rails (510), and the short arms of any L-shaped plate (511) are fixedly connected to the top surfaces of the adjacent guide rails (510). A third bearing (550) is provided above the ring body (120), and a plurality of movable grooves (551) are provided on the bottom surface of the inner ring of the third bearing (550). A movable plate (560) is provided above the plurality of guide rails (510), and the plurality of movable grooves (551) correspond to the plurality of movable plates (560) one by one, and the top ends of the plurality of movable plates (560) are slidably engaged in the corresponding movable grooves (551), and the bottom surfaces of the plurality of movable plates (560) are provided with movable plates (560). Two sliding rods (570) are provided, and any sliding rod (570) is slidably engaged between the short arms of two adjacent L-shaped plates (511). The outer wall of any sliding rod (570) is fixedly connected with two supporting rods, and the two supporting rods on any sliding rod (570) are rotatably connected to the bottom end of the adjacent movable plate (560). The plurality of movable plates (560) are matched with rotating rings (571), and a circular through hole is opened on one side of the plurality of rotating rings (571), and the plurality of circular through holes are rotatably sleeved with the outer wall of an adjacent sliding rod (570), and the inner walls of the plurality of rotating rings (571) are slidably sleeved with the outer wall of the adjacent vacuum tube (521).
2. The automatic layered loading device for a centrifuge according to claim 1, characterized in that: The top surface of the housing (200) is provided with two installation openings, and a fixed cylinder (450) is fixedly connected to the center of the inner top surface of the housing (200), and two electric push rods (451) are fixedly connected to the bottom surface of the fixed cylinder (450), and the movable ends of the two electric push rods (451) are fixedly connected to the top surface of the outer ring of the first bearing (440).
3. The automatic layered loading device for a centrifuge according to claim 1, characterized in that: Two hydraulic cylinders (552) are fixedly connected to the inner top surface of the housing (200), and the movable ends of the two hydraulic cylinders (552) are fixedly connected to the top surface of the outer ring of the third bearing (550).
Citation Information
Patent Citations
Solution self-separation centrifugal device for gene detection
CN114453146A
Centrifugal liquid separation device for inspection test tube
CN115069427A