Freezing, storing and transporting system for sheep semen and using method thereof

Abstract

According to the freezing, storing and conveying system for the sheep semen and the using method of the freezing, storing and conveying system, the storing and conveying pipe containing the rubber piston is adopted and can be used in cooperation with different assemblies in the using process, and the semen is sucked and stored when the storing and conveying pipe is used in cooperation with a split-charging communicating structure and an injector; when the ewe insemination device is combined with a pushing structure to be used, the ewe insemination device can go deep into the ewe to conduct insemination operation, simplified semen split charging and storage operation and insemination operation are integrally achieved, and compared with the prior art, the production cost and the insemination safety are greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of artificial insemination of sheep, in particular to a freezing storage and transportation system for sheep semen and a use method thereof. Background Art

[0002] Artificial insemination is the most efficient and high-quality method for sheep breeding. Artificial insemination technology refers to the method of using instruments to collect semen from rams, and then using instruments to transport the semen to the reproductive tract of estrus ewes to make them pregnant after processing and preservation. Artificial insemination technology can quickly expand the number of good breeds and effectively improve the utilization rate of good breeding rams. It is one of the important technologies in modern animal husbandry. Artificial insemination technology for sheep includes semen collection and processing, preservation, estrus identification and timely insemination technology.

[0003] During the storage process of sheep semen, freezing technology can be used to preserve semen for a long time, reduce the cost of live seed preservation, and prevent gene drift. Semen freezing technology uses liquid nitrogen (-196℃), dry ice (-79℃) and other cold sources to store semen in ultra-low temperature after special treatment, reduce and inhibit the metabolic rate and intensity of sperm, and prolong the survival time of sperm in vitro to achieve the purpose of long-term preservation. It is an advanced livestock breeding technology. It is a major innovation in artificial insemination technology, which can make semen not limited by time and region and can be stored for a long time.

[0004] The existing artificial insemination technology of frozen semen in sheep is to use 0.25mm expanded cotton plug cap to make frozen semen in a cap, and then use a conventional insemination gun to inseminate after thawing. Since sheep are small animals, they can only be inseminated by the vaginal opening method. Due to the limitation of the structure of the sheep cervix, the current frozen semen tube and its matching insemination gun cannot reach the ideal insemination depth, causing the conception rate of frozen semen in sheep to hover between 35% and 45% for a long time, which has become a global problem, making the popularization and promotion of artificial insemination technology in sheep far behind that of cattle, pigs and other livestock. Summary of the invention

[0005] The present invention relates to a freezing storage and transportation system for sheep semen and a method for using the same. The system adopts a storage and transportation tube including a rubber piston, which can be used in conjunction with different components. When used in conjunction with a packaging connecting structure and a syringe, the semen can be absorbed and stored. When used in conjunction with a pushing structure, the system can penetrate into the body of a ewe for insemination. As a whole, simplified semen storage and transportation operations and insemination operations are achieved, and both production costs and insemination safety are greatly improved compared to the prior art.

[0006] The present invention adopts the following technical solutions to achieve the above purpose: A freezing storage and transportation system for sheep semen, comprising: The storage and delivery tube is a tubular structure with a rubber piston inside. The rubber piston preserves the semen drawn into the storage and delivery tube and pushes the semen out of the storage and delivery tube during insemination. Sealing head, which seals the end of the storage and transportation pipe and covers the outer wall of the storage and transportation pipe to prevent the storage and transportation pipe from being contaminated; The dispensing connecting part is used to connect the storage tube and the syringe, and then the semen is sucked into the storage tube through the syringe. A pin is provided inside to push the rubber piston. After the semen is extracted, the rubber piston is pushed to the corresponding position; The push part is connected to the tail of the storage and delivery tube during insemination, pushes the rubber piston in the storage and delivery tube to push out the semen in the storage and delivery tube.

[0007] The storage and delivery tube is a tubular structure as a whole, and is composed of three sections, namely, a sperm delivery head, a liquid storage chamber and a tube tail; a conical nozzle is provided at the end of the sperm delivery head, and a penetrating capillary is provided in the sperm delivery head; the liquid storage chamber is located in the middle of the storage and delivery tube, and the diameter of the liquid storage chamber is greater than the diameter of the capillary; the liquid storage chamber penetrates backward to the tube tail; the rubber piston is movably inserted in the tube tail.

[0008] Furthermore, evenly distributed conducting grooves are provided on the inner wall of the storage and delivery pipe at the pipe tail, and the conducting grooves are of an inwardly concave structure; the rubber piston is of a columnar structure as a whole, and the two ends of the rubber piston are of an inwardly retracted conical structure; the rubber piston corresponds to the frontmost part of the conducting groove, and when the rubber piston is at the front part of the conducting groove, the liquid storage chamber and the pipe tail are connected through the conducting groove.

[0009] The sealing head is fitted with the outer wall of the sperm delivery head of the storage and delivery tube, and a plugging needle corresponding to the capillary in the sperm delivery head is arranged on the inner side of the end of the sealing head.

[0010] The sub-packaging connecting part is a tubular structure, and an annular baffle is arranged in the sub-packaging connecting part, which divides the sub-packaging connecting part into a syringe plug hole and a storage tube plug hole; a cylindrical ejector is connected to the baffle, the ejector faces one side of the storage tube plug hole, and the ejector, the syringe plug hole and the storage tube plug hole are concentric structures; the opening of the ejector is connected to the syringe plug hole, and a vent hole is arranged on the side wall of the ejector; an annular sealing ring is arranged at the opening of the storage tube plug hole, and the sealing ring is made of rubber material.

[0011] The pushing part includes a main body tube and a push rod arranged in the main body tube, a stepped through hole is arranged in the main body tube, the diameter of the front half of the main body tube is larger than the diameter of the rear half; the tail tube of the storage and transportation tube is inserted into the front half of the main body tube; an ear protrusion is arranged on the outer wall of the storage and transportation tube at the tail of the tube, and a side hole corresponding to the ear protrusion is arranged on the front half of the main body tube; a handle is arranged at the tail of the main body tube, and the push rod is a straight rod structure, and the push rod is inserted into the rear half of the main body tube.

[0012] The storage and delivery tube has an outer diameter of 3mm and a total length of 85~90mm, wherein the sperm delivery head is 35mm long, the inner diameter of the capillary is 0.2mm, and the nozzle is tapered to a diameter of 2mm; the liquid storage chamber is 40~45mm long and has an inner diameter of 2mm; the tube tail is 10~15mm long, the ear is 0.5mm high and 5mm long; the rubber piston is 5mm long, the middle diameter is 3.1mm, and the two ends are retracted to a diameter of 1mm; the sealing head has an outer diameter of 3.5mm, an inner diameter of 3mm, and the plug needle is 3mm long and has an outer diameter of 0.2mm; the ejector pin of the dispensing and connecting part is 15mm long and has a diameter of 1.6mm; the main tube of the pushing part is 190~220mm long and has an outer diameter of 4.4mm, an inner diameter of 3.2mm in the front section, and an inner diameter of 2mm in the rear section. The side hole is located at 45mm~50mm in the front section of the main tube, and the push rod is 200~230mm long and has a diameter of 1.6mm.

[0013] A method for using a frozen storage and transportation system for sheep semen, comprising the following steps: 1. Preparation of frozen semen in storage and delivery tube 1) Prepare several disposable storage and transportation pipes and sealing heads; 2) Connect the dispensing connection part to the quantitative syringe and adjust the dosage of the quantitative syringe; 3) Insert the tail of the storage tube into the sealing ring of the dispensing connection part, press the piston of the quantitative syringe to exhaust the air, and insert the sperm delivery head of the storage tube into the cup for diluting semen. After releasing the pressure, the semen is sucked into the storage tube under the action of negative pressure; 4) Push the storage and delivery tube into the storage and delivery tube jack of the dispensing connecting part with force. The ejector pushes the rubber piston in the storage and delivery tube forward to the liquid storage chamber. There is no conduction groove in the liquid storage chamber, and the rubber piston has a sealing effect. 5) Remove the storage and delivery tube, and then insert the sperm delivery head of the storage and delivery tube into the sealing head to complete the sealing of the storage and delivery tube; 6) Balance the encapsulated storage and transportation tubes and store them in a frozen state.

[0014] 2. Use of frozen semen storage tube 1) Thawing of frozen semen in a storage tube: Use tweezers to take a frozen semen in a storage tube and quickly immerse it in 38℃~40℃ water and shake it. Take it out after 7 seconds and wipe off the water droplets with absorbent paper or gauze. 2) Connection between the storage and transportation tube and the push part: insert the tail end of the thawed storage and transportation tube into the front section of the main tube of the push part, and insert the ear into the side hole to complete the installation; 3) Insemination: Before insemination, remove the sealing head, insert the vaginal opener into the vagina of the estrus ewe and find the cervical opening, then insert the insemination head of the storage tube into the ewe's cervix, press the push rod cap to make the rubber piston reach the end of the liquid storage chamber, and the insemination is completed; 4) Collection and treatment of storage and transportation tubes: Press the push rod cap hard to disengage the ear protrusion from the side hole, pull out the storage and transportation tube and put it into a collection bag, and then dispose of it harmlessly.

[0015] The present invention adopts the above technical solution to achieve the following beneficial effects: 1. Storage and transportation pipes are easy to mass produce, low cost, and disposable products are beneficial to biosafety; 2. Unlike the existing straw-tube frozen semen, it no longer requires a gun jacket during insemination. First, it saves resources. Second, the insemination head is thinner, which makes it easier to achieve deep insemination. 3. Save semen. The existing tampon tube relies on the gelatin in the tampon to absorb a small amount of semen and expand to complete the sealing, which wastes some semen; 4. During the insemination process, only the insemination head directly contacts the sheep's body. Before insemination, the insemination head is always shielded by the sealing head, which increases safety; 5. Based on the above benefits, the conception rate of frozen sperm in sheep is significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the storage and transportation pipeline.

[0017] Figure 2 for Figure 1 Schematic diagram of the cross section at point A.

[0018] Figure 3 Schematic diagram of the sealing head.

[0019] Figure 4 It is a schematic diagram of the subpackaging connecting part.

[0020] Figure 5 This is a schematic diagram of the push unit.

[0021] Figure 6 It is a schematic diagram of the connection between the storage and delivery tube, the filling connecting part and the syringe.

[0022] Figure 7 Schematic diagram of semen suction through the reservoir tube.

[0023] Figure 8 This is a schematic diagram of the storage and transportation pipe being installed on the pushing part.

[0024] Fig. 9 Schematic diagram of the reservoir and pusher tubes after insemination.

[0025] In the figure, 1. Storage and delivery tube; 101. Rubber piston; 102. Sperm delivery head; 103. Liquid storage chamber; 104. Tube tail; 105. Capillary tube; 106. Conducting groove; 107. Ear protrusion; 2. Sealing head; 201. Plug needle; 3. Packing connecting part; 301. ejector pin; 302. baffle; 303. syringe plug hole; 304. storage and delivery tube plug hole; 305. vent hole; 306. sealing ring; 4. Pushing part; 401. Main body tube; 402. Push rod; 403. Through hole; 404. Side hole; 405. Handle; 406. Push rod cap. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with the accompanying drawings and examples. It should be clear that the following examples are only one or more manifestations of the present invention and are not a complete limitation of the invention described in this application. Any improvements known to those skilled in the art made on the basis of the invention or technical solutions described in this application should fall within the scope of protection claimed in this application.

[0027] In addition, the expressions of up, down, left, right, front, back, etc. presented in the specific implementation manner are only expressed to facilitate the description of the technical solution and are not absolute limitations unless otherwise specified.

[0028] A freezing storage and transportation system for sheep semen, comprising: like Figure 1 and Figure 2 The storage and delivery tube 1 shown is a tubular structure, and a rubber piston 101 is provided inside. The rubber piston 101 preserves the semen drawn into the storage and delivery tube 1 and pushes the semen out of the storage and delivery tube 1 during insemination. like Figure 3 The sealing head 2 shown in the figure blocks the end of the storage and transportation pipe 1 and covers the outer wall of the storage and transportation pipe 1 to prevent the storage and transportation pipe 1 from being contaminated; like Figure 4 The dispensing connecting part 3 shown is used to connect the storage tube 1 and the syringe 5, and then the semen is sucked into the storage tube 1 through the syringe 5. A push pin 301 is provided inside to push the rubber piston 101. After the semen is extracted, the rubber piston 101 is pushed to the corresponding position. like Figure 5 The pushing portion 4 shown is connected to the tail end of the storage and delivery tube 1 during insemination, and pushes the rubber piston 101 in the storage and delivery tube 1 to push out the semen in the storage and delivery tube 1.

[0029] like Figure 1 and Figure 2As shown, the storage and delivery tube 1 is a tubular structure as a whole, and is composed of three sections, namely, a sperm delivery head 102, a liquid storage chamber 103 and a tube tail 104; a conical nozzle is provided at the end of the sperm delivery head 102, and a penetrating capillary 105 is provided in the sperm delivery head 102; the liquid storage chamber 103 is located in the middle of the storage and delivery tube 1, and the diameter of the liquid storage chamber 103 is greater than the diameter of the capillary 105; the liquid storage chamber 103 penetrates backward to the tube tail 104; the rubber piston 101 is movably inserted in the tube tail 104.

[0030] Furthermore, evenly distributed conducting grooves 106 are provided on the inner wall of the storage and delivery pipe 1 at the pipe tail 104, and the conducting grooves 106 are of a concave structure; the rubber piston 101 is of a columnar structure as a whole, and the two ends of the rubber piston 101 are of an inwardly retracted conical structure; the rubber piston 101 corresponds to the frontmost part of the conducting groove 106, and when the rubber piston 101 is in front of the conducting groove 106, the liquid storage chamber 103 and the pipe tail 104 are connected through the conducting groove 106.

[0031] like Figure 3 The sealing head 2 shown is fitted with the outer wall of the sperm delivery head 102 of the storage and delivery tube 1, and a plugging needle 201 corresponding to the capillary 105 in the sperm delivery head 102 is provided on the inner side of the end of the sealing head 2.

[0032] like Figure 4 The sub-packaging connecting portion 3 shown is a tubular structure, and an annular baffle 302 is provided in the sub-packaging connecting portion 3, and the baffle 302 divides the sub-packaging connecting portion 3 into a syringe socket 303 and a storage tube socket 304; a cylindrical ejector pin 301 is connected to the baffle 302, and the ejector pin 301 faces the side of the storage tube socket 304, and the ejector pin 301 is a concentric structure with the syringe socket 303 and the storage tube socket 304; the opening of the ejector pin 301 is connected to the syringe socket 303, and a vent hole 305 is provided on the side wall of the ejector pin 301; an annular sealing ring 306 is provided at the opening of the storage tube socket 304, and the sealing ring 306 is made of rubber material.

[0033] like Figure 5 The push portion 4 shown includes a main tube 401 and a push rod 402 arranged in the main tube 401, the main tube 401 is provided with a stepped through hole 403, the diameter of the front half of the main tube 401 is larger than the diameter of the rear half; the tail tube of the storage and transportation tube 1 is inserted into the front half of the main tube 401; an ear protrusion 107 is provided on the outer wall of the storage and transportation tube 1 at the tube tail 104, and a side hole 404 corresponding to the ear protrusion 107 is provided on the front half of the main tube 401; a handle 405 is provided at the tail of the main tube 401, the push rod 402 is a straight rod structure, the push rod 402 is inserted into the rear half of the main tube 401, and a push rod cap 406 is provided at the tail of the push rod 402.

[0034] The storage and delivery tube has an outer diameter of 3mm and a total length of 85~90mm, wherein the sperm delivery head is 35mm long, the inner diameter of the capillary is 0.2mm, and the nozzle is tapered to a diameter of 2mm; the liquid storage chamber is 40~45mm long and has an inner diameter of 2mm; the tube tail is 10~15mm long, the ear is 0.5mm high and 5mm long; the rubber piston is 5mm long, the middle diameter is 3.1mm, and the two ends are retracted to a diameter of 1mm; the sealing head has an outer diameter of 3.5mm, an inner diameter of 3mm, and the plug needle is 3mm long and has an outer diameter of 0.2mm; the ejector pin of the dispensing and connecting part is 15mm long and has a diameter of 1.6mm; the main tube of the pushing part is 190~220mm long and has an outer diameter of 4.4mm, an inner diameter of 3.2mm in the front section, and an inner diameter of 2mm in the rear section. The side hole is located at 45mm~50mm in the front section of the main tube, and the push rod is 200~230mm long and has a diameter of 1.6mm.

[0035] The device mainly involves two parts of operation when in use. One part is to absorb the semen and freeze it for preservation, and the other part is to operate the storage and delivery tube for cryopreservation. The details are as follows: 1. Preparation of frozen semen in storage and delivery tube 1) Prepare several disposable storage and transportation pipes and sealing heads; 2) If Figure 6 As shown, connect the dispensing connecting part with the quantitative syringe, and adjust the dosage of the quantitative syringe; 3) Insert the tail of the storage tube into the sealing ring of the dispensing connection part, press the piston of the quantitative syringe to exhaust the air, and insert the sperm delivery head of the storage tube into the cup for diluting semen. After releasing the pressure, the semen is sucked into the storage tube under the action of negative pressure; 4) Push the storage and delivery tube into the storage and delivery tube jack of the dispensing connecting part with force. The ejector pushes the rubber piston in the storage and delivery tube forward to the liquid storage chamber. There is no conduction groove in the liquid storage chamber, and the rubber piston has a sealing effect. 5) Remove the storage and delivery tube, and then insert the sperm delivery head of the storage and delivery tube into the sealing head. Figure 7 The packaging of a storage and transportation tube shown; 6) Balance the encapsulated storage and transportation tubes and store them in a frozen state.

[0036] 2. Use of frozen semen storage tube 1) Thawing of frozen semen in a storage tube: Use tweezers to take a frozen semen in a storage tube and quickly immerse it in 38℃~40℃ water and shake it. Take it out after 7 seconds and wipe off the water droplets with absorbent paper or gauze. 2) Connection between the storage and transport pipe and the push unit: Figure 8 As shown in the figure, insert the tail end of the thawed storage and transportation tube into the front section of the main tube of the push part, and insert the ear protrusion into the side hole to complete the installation; 3) Insemination: Before insemination, remove the sealing head, insert the vaginal opener into the vagina of the estrus ewe and find the cervical opening, then insert the insemination head of the storage tube into the cervix of the ewe, press the push rod cap to make the rubber piston reach the end of the liquid storage chamber, and the insemination is completed. The state after insemination is as follows: Fig. 9 As shown; 4) Collection and treatment of storage and transportation tubes: Press the push rod cap hard to disengage the ear protrusion from the side hole, pull out the storage and transportation tube and put it into a collection bag, and then dispose of it harmlessly.

[0037] The storage and transportation tube in this system is a disposable item with a relatively simple structure and a low overall cost. The other supporting tools, such as syringes, decomposition connecting parts, and pushing parts, can be used repeatedly. These supporting tools do not come into direct contact with semen, so that no contamination will be caused during the semen absorption and insemination process, thereby maximizing safety.

[0038] During use, the storage and delivery tube is used as a carrier for sucking semen and insemination, and also as a storage container for semen, realizing a closed loop of suction-frozen storage-insemination. In the entire closed loop, the semen does not come into contact with external instruments, reducing the chance of semen coming into contact with the outside world, especially from the thawing of frozen semen to the insemination stage, the semen in the storage and delivery tube does not come into contact with the outside world, reducing the risk of semen contamination in the process to zero.

Claims

1. A freezing storage and transportation system for sheep semen, Features: It includes: The storage and delivery tube is a tubular structure with a rubber piston inside. The rubber piston preserves the semen drawn into the storage and delivery tube and pushes the semen out of the storage and delivery tube during insemination. Sealing head, which seals the end of the storage and transportation pipe and covers the outer wall of the storage and transportation pipe to prevent the storage and transportation pipe from being contaminated; The dispensing connecting part is used to connect the storage tube and the syringe, and then the semen is sucked into the storage tube through the syringe. A pin is provided inside to push the rubber piston. After the semen is extracted, the rubber piston is pushed to the corresponding position; The push part is connected to the tail of the storage and delivery tube during insemination, pushes the rubber piston in the storage and delivery tube to push out the semen in the storage and delivery tube.

2. A freezing storage and transportation system for sheep semen according to claim 1, Features: The storage and delivery tube is a tubular structure as a whole, and is composed of three sections, namely, a sperm delivery head, a liquid storage chamber and a tube tail; a conical nozzle is provided at the end of the sperm delivery head, and a penetrating capillary is provided in the sperm delivery head; the liquid storage chamber is located in the middle of the storage and delivery tube, and the diameter of the liquid storage chamber is greater than the diameter of the capillary; the liquid storage chamber penetrates backward to the tube tail; the rubber piston is movably inserted in the tube tail.

3. A freezing storage and transportation system for sheep semen according to claim 2, Features: The inner wall of the storage and delivery pipe at the pipe tail is provided with evenly distributed conducting grooves, and the conducting grooves are of an inwardly concave structure; the rubber piston is of a columnar structure as a whole, and the two ends of the rubber piston are of an inwardly retracted conical structure; the rubber piston corresponds to the frontmost part of the conducting groove, and when the rubber piston is at the front part of the conducting groove, the liquid storage chamber and the pipe tail are connected through the conducting groove.

4. A freezing storage and transportation system for sheep semen according to claim 3, Features: The sealing head is fitted with the outer wall of the sperm delivery head of the storage and delivery tube, and a plugging needle corresponding to the capillary in the sperm delivery head is arranged on the inner side of the end of the sealing head.

5. A freezing storage and transportation system for sheep semen according to claim 4, Features: The sub-packaging connecting part is a tubular structure, and an annular baffle is arranged in the sub-packaging connecting part, which divides the sub-packaging connecting part into a syringe plug hole and a storage tube plug hole; a cylindrical ejector is connected to the baffle, the ejector faces one side of the storage tube plug hole, and the ejector, the syringe plug hole and the storage tube plug hole are concentric structures; the opening of the ejector is connected to the syringe plug hole, and a vent hole is arranged on the side wall of the ejector; an annular sealing ring is arranged at the opening of the storage tube plug hole, and the sealing ring is made of rubber material.

6. A freezing storage and transportation system for sheep semen according to claim 5, Features: The pushing part includes a main body tube and a push rod arranged in the main body tube, a stepped through hole is arranged in the main body tube, the diameter of the front half of the main body tube is larger than the diameter of the rear half; the tail tube of the storage and transportation tube is inserted into the front half of the main body tube; an ear protrusion is arranged on the outer wall of the storage and transportation tube at the tail of the tube, and a side hole corresponding to the ear protrusion is arranged on the front half of the main body tube; a handle is arranged at the tail of the main body tube, and the push rod is a straight rod structure, and the push rod is inserted into the rear half of the main body tube.

7. A freezing storage and transportation system for sheep semen according to claim 6, Features: The storage and delivery tube has an outer diameter of 3mm and a total length of 85~90mm, wherein the sperm delivery head is 35mm long, the inner diameter of the capillary is 0.2mm, and the nozzle is tapered to a diameter of 2mm; the liquid storage chamber is 40~45mm long and has an inner diameter of 2mm; the tube tail is 10~15mm long, the ear is 0.5mm high and 5mm long; the rubber piston is 5mm long, the middle diameter is 3.1mm, and the two ends are retracted to a diameter of 1mm; the sealing head has an outer diameter of 3.5mm, an inner diameter of 3mm, and the plug needle is 3mm long and has an outer diameter of 0.2mm; the ejector pin of the dispensing and connecting part is 15mm long and has a diameter of 1.6mm; the main tube of the pushing part is 190~220mm long and has an outer diameter of 4.4mm, an inner diameter of 3.2mm in the front section, and an inner diameter of 2mm in the rear section. The side hole is located at 45mm~50mm in the front section of the main tube, and the push rod is 200~230mm long and has a diameter of 1.6mm.

8. A method for using a frozen storage and transportation system for sheep semen, which includes two parts: making frozen semen in a storage and transportation tube and using the frozen semen in the storage and transportation tube.

9. A method for using the frozen storage and transportation system for sheep semen according to claim 8, Features: The steps for making frozen semen in a storage tube are as follows: 1) Prepare several disposable storage and transportation pipes and sealing heads; 2) Connect the dispensing connection part to the quantitative syringe and adjust the dosage of the quantitative syringe; 3) Insert the tail of the storage tube into the sealing ring of the dispensing connection part, press the piston of the quantitative syringe to exhaust the air, and insert the sperm delivery head of the storage tube into the cup for diluting semen. After releasing the pressure, the semen is sucked into the storage tube under the action of negative pressure; 4) Push the storage and delivery tube into the storage and delivery tube jack of the dispensing connecting part with force. The ejector pushes the rubber piston in the storage and delivery tube forward to the liquid storage chamber. There is no conduction groove in the liquid storage chamber, and the rubber piston has a sealing effect. 5) Remove the storage and delivery tube, and then insert the sperm delivery head of the storage and delivery tube into the sealing head to complete the sealing of the storage and delivery tube; 6) Balance the encapsulated storage and transportation tubes and store them in a frozen state.

10. A method for using the frozen storage and transportation system for sheep semen according to claim 8, Features: The steps for using frozen semen in a storage tube are as follows: 1) Thawing of frozen semen in a storage tube: Use tweezers to take a frozen semen in a storage tube and quickly immerse it in 38℃~40℃ water and shake it. Take it out after 7 seconds and wipe off the water droplets with absorbent paper or gauze. 2) Connection between the storage and transportation tube and the push part: insert the tail end of the thawed storage and transportation tube into the front section of the main tube of the push part, and insert the ear into the side hole to complete the installation; 3) Insemination: Before insemination, remove the sealing head, insert the vaginal opener into the vagina of the estrus ewe and find the cervical opening, then insert the insemination head of the storage tube into the ewe's cervix, press the push rod cap to make the rubber piston reach the end of the liquid storage chamber, and the insemination is completed; 4) Collection and treatment of storage and transportation tubes: Press the push rod cap hard to disengage the ear protrusion from the side hole, pull out the storage and transportation tube and put it into a collection bag, and then dispose of it harmlessly.

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