A silk fibroin emulsion containing oryzanol and application thereof

Abstract

The present invention relates to a silk fibroin emulsion containing oryzanol and its application, and belongs to the technical field of nanoemulsion. The silk fibroin emulsion of the present invention has a water content of 575 to 635 parts, a camellia oil content of 350 to 400 parts, a silk fibroin content of 12 to 15 parts, and a oryzanol content of 3 to 10 parts by mass; the particle size of the silk fibroin emulsion is within 320.74nm. In the present invention, the appropriate addition of oryzanol can reduce the particle size of the emulsion, improve the stability of the silk fibroin emulsion, thereby improving the application value of the silk fibroin emulsion, so that the performance of many cosmetics is improved after the emulsion is added, and a wider space is opened up for the application of the silk fibroin emulsion.

Description

Technical Field

[0001] The invention belongs to the technical field of nanoemulsions and relates to a silk fibroin emulsion containing oryzanol and application thereof. Background Art

[0002] Silk fibroin is a natural polymer protein extracted from silk. It is a non-toxic, non-irritating natural biomaterial with good mechanical properties and unique biological activity. It has good biocompatibility and degradability, good air and moisture permeability, low immunogenicity, etc. In recent years, it has been widely used in biomedicine, cosmetics, and food industries.

[0003] Oryzanol exists in many vegetable oils, such as rice bran oil, corn germ oil, wheat germ oil, barley bran oil, and rapeseed oil. Among them, the oryzanol content in rice bran oil is the highest, generally between 1.4% and 2.9%. In industry, a lot of oryzanol is extracted from crude rice bran oil. Rice bran oil is rice oil made from rice bran. The methods for extracting oryzanol from rice bran oil mainly include direct solvent extraction and extraction after rice bran oil saponification. After alkali saponification of rice bran oil, most of the oryzanol will be enriched in the soap stock. Initially, oryzanol was considered to be a single compound, but with the gradual diversification of detection and analysis methods, it was proved that the main body of oryzanol is cycloartanol, which is a mixture of ferulic acid esters with cycloartanol as the main body and sterol ferulic acid esters, and the content of cycloartanol ferulic acid esters is 75%~80%.

[0004] Oryzanol is mainly used clinically to treat neurosis, premenstrual tension syndrome, and menopausal syndrome as a sedative and sleep aid. The application of oryzanol in the food field includes oryzanol-rich edible oil, oryzanol beverages, oryzanol capsules, etc. Oryzanol-rich edible oil has been proven to have significant therapeutic effects on patients with hypertension in clinical trials. Oryzanol is often used as an antioxidant in food additives to prevent lipid rancidity and extend the shelf life. Compared with artificial synthetic antioxidants, it is more in line with modern people's pursuit of natural and healthy food.

[0005] As a natural substance, oryzanol has a high application value in cosmetics due to its low irritation, safety and effectiveness. In the field of cosmetics, oryzanol can protect the skin, prevent skin dehydration, prevent skin keratinization and repair the skin; oryzanol can also improve skin inflammation caused by menopause; resist skin lipid oxidation, absorb ultraviolet rays, promote pigment stability, and prevent the formation of erythema caused by sunlight; inhibit tyrosinase activity and prevent the production of melanin; promote peripheral blood vessel circulation and increase local skin temperature; and activate the symptoms of sebaceous gland atrophy and dysfunction. Summary of the invention

[0006] The purpose of the present invention is to overcome the shortcomings and deficiencies of the large particle size of the nanoemulsion leading to the instability of the emulsion and the short shelf life of the emulsion. The present invention utilizes oryzanol to reduce the particle size of the silk fibroin emulsion, thereby increasing the shelf life of the emulsion.

[0007] In a first aspect, the present invention provides a silk fibroin emulsion containing oryzanol, wherein the silk fibroin emulsion, by weight, contains 575 to 635 parts of water, 350 to 400 parts of camellia oil, 12 to 15 parts of silk fibroin, and 3 to 10 parts of oryzanol;

[0008] The particle size of the silk fibroin emulsion is within 320.74 nm.

[0009] Furthermore, the emulsion of the present invention has, by weight, 575 to 633 parts of water, 350 to 400 parts of camellia oil, 12 to 15 parts of silk fibroin, and 5 to 10 parts of oryzanol;

[0010] The particle size of the silk fibroin emulsion is within 300.03 nm.

[0011] In addition, the present invention also provides a silk fibroin emulsion containing oryzanol, wherein, by weight, the water content is 522 parts, the camellia oil content is 450 parts, the silk fibroin content is 18 parts, and the oryzanol content is 10 parts;

[0012] The particle size of the silk fibroin emulsion is 305.36 nm.

[0013] In a second aspect, the present invention provides the use of the above-mentioned silk fibroin emulsion containing oryzanol in cosmetic raw materials.

[0014] In a third aspect, the present invention provides the use of the above-mentioned silk fibroin emulsion containing oryzanol in any product such as lotion, facial mask, lotion or cream.

[0015] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0016] The invention provides the application of oryzanol in preparing silk fibroin emulsion. By adding oryzanol, the particle size of the silk fibroin emulsion is reduced, the stability and uniformity of the silk fibroin emulsion are improved, and the use of oryzanol, a natural substance, as an emulsifying auxiliary agent has good safety.

[0017] The nanoemulsion provided by the present invention has the characteristics of high encapsulation rate, high stability, and controlled release of active substances, and can better improve the performance of cosmetics. The nanoemulsion can help improve the water solubility and bioavailability of poorly soluble drugs, and the small and uniform particle size helps to improve the stability of the emulsion, and can also improve the emulsion stratification and improve stability.

[0018] The present invention uses the silk fibroin emulsion with optimized particle size as a raw material for the preparation of cosmetics, which can improve the performance of the cosmetics and open up new possibilities for developing cosmetic products with specific particle size requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a particle size diagram of the silk fibroin emulsion after adding oryzanol to the oil phase described in Example 4. DETAILED DESCRIPTION

[0020] The technical scheme of the present invention is described below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0021] The present invention provides a method for preparing a oryzanol silk fibroin emulsion, which is specifically as follows:

[0022] Place oryzanol and camellia oil in a 60 ℃ water bath and heat for 30 min. Stir during heating to make oryzanol completely dissolved in camellia oil to obtain oil phase. Dissolve silk fibroin in water to obtain water phase. Shear homogenize the oil phase and water phase at 5000 rpm for 10 min to obtain colostrum. Cycle the colostrum with 400 bar high pressure homogenization once and then 700 bar for four times to obtain a uniform and delicate emulsion. The prepared emulsion was tested for its emulsion particle size by the Biotek nanoparticle size potentiometer.

[0023] The silk fibroin emulsions provided in Examples 1 to 10 and Comparative Examples 1 to 3 were all prepared by the above method.

[0024] The formulas of the silk fibroin emulsions provided in Examples 1 to 4 and Comparative Example 1 are shown in Table 1.

[0025] Table 1 Silk fibroin emulsion formula 1

[0026]

[0027] The formulas of the silk fibroin emulsions provided in Examples 5 to 7 and Comparative Example 2 are shown in Table 2.

[0028] Table 2 Silk fibroin emulsion formula 2

[0029]

[0030] The formulas of the silk fibroin emulsions provided in Examples 8 to 10 and Comparative Example 3 are shown in Table 3.

[0031] Table 3 Silk fibroin emulsion formula 3

[0032]

[0033] The particle sizes of the silk fibroin emulsions provided in Examples 1 to 10 and Comparative Examples 1 to 3 are shown in Table 4.

[0034] Table 4 Results of emulsion particle size detection by the Nano Particle Size Potential Analyzer

[0035]

[0036] According to the results in Table 4, when the addition amount of camellia oil in the silk fibroin emulsion is 40%, the addition amount of silk fibroin is 1.5%, and the addition amount of oryzanol in the emulsion increases from 0 to 1% (Examples 1 to 4 and Comparative Example 1), the particle size of the silk fibroin emulsion decreases from 391.04 nm to 291.47 nm, indicating that the addition amount of oryzanol significantly affects the particle size of the silk fibroin emulsion, that is, the more oryzanol is added, the smaller the particle size of the silk fibroin emulsion, and Examples 5 to 7 and Comparative Example 2, Examples 8 to 10 and Comparative Example 3 also show similar results. Figure 1 The figure is a test result diagram of the particle size of the silk fibroin emulsion prepared in Example 4. The particle size data of Example 4 in Table 4 is the average value of the test results, which further verifies that the addition of oryzanol can reduce the particle size of the emulsion, and the higher the proportion of oryzanol relative to the mass of the silk fibroin emulsion, the smaller the particle size.

[0037] Table 5 Stability of silk fibroin emulsion

[0038]

[0039] The silk fibroin emulsions prepared in Example 3 and Comparative Example 1 were stored at 48°C for 2 months, and then returned to room temperature to observe the appearance and fluidity of the silk fibroin emulsions. As shown in Table 5, after oryzanol was added to the silk fibroin emulsion (Example 3), even if the silk fibroin emulsion was stored in a high temperature environment for a long time, the silk fibroin emulsion still maintained a high uniformity of the water-oil interface, had no obvious stratification, and had good fluidity. In contrast, the silk fibroin emulsion (Comparative Example 1) to which oryzanol was not added showed obvious stratification, and its fluidity decreased significantly. The above results show that oryzanol can be used as an additive to significantly improve the stability and fluidity of the silk fibroin emulsion, so that it can still maintain good stability in a high-temperature storage environment, providing technical support for the application of silk fibroin emulsion in the field of cosmetics.

[0040] Embodiment 11

[0041] This embodiment provides the application of silk fibroin emulsion in cosmetic products.

[0042] In this example, the silk fibroin emulsion prepared in Example 3 was used as a raw material to prepare products such as lotion, facial mask, lotion and cream. The formulas of the lotion, facial mask, lotion and cream are shown in Tables 6 to 9.

[0043] The silk fibroin emulsion prepared in Example 3 was used as a raw material for lotion to prepare lotion. The formula composition of the lotion is shown in Table 6:

[0044] Table 6 Formula of lotion

[0045]

[0046] The silk fibroin emulsion prepared in Example 3 was used as a raw material for a facial mask liquid to prepare a facial mask liquid. The formula composition of the facial mask liquid is shown in Table 7:

[0047] Table 7 Formula of facial mask liquid

[0048]

[0049] The silk fibroin emulsion prepared in Example 3 was used as the raw material of the emulsion to prepare the emulsion. The formula composition of the emulsion is shown in Table 8:

[0050] Table 8 Emulsion formula

[0051]

[0052] The silk fibroin emulsion prepared in Example 3 was used as a raw material for a cream to prepare the cream. The formula composition of the cream is shown in Table 9:

[0053] Table 9 Cream formula

[0054]

[0055] This embodiment provides the application of silk fibroin emulsion in the field of cosmetics, including lotion, facial mask, lotion and cream, etc. The lotion, facial mask, lotion and cream prepared according to the formula of the present invention have excellent stability, increase the anti-coagulation ability of the product, and make the above cosmetics have better performance.

[0056] That is to say, the addition of oryzanol in the present invention improves the stability of the emulsion and promotes the formation of finer and more uniform emulsion particles. In addition, the emulsification-aiding effect of oryzanol may also affect the microstructure of the emulsion, further optimizing the physical properties of the emulsion. The optimized emulsion can be used to prepare cosmetics with any specific particle size requirements, and the stability and shelf life of the cosmetics can also be improved.

[0057] The embodiments described above are part of the embodiments of the present invention, but not all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. A silk fibroin emulsion containing oryzanol, characterized in that: The silk fibroin emulsion is composed of the following components in parts by mass: 575 parts to 635 parts of water, 350 parts to 400 parts of camellia oil, 12 parts to 15 parts of silk fibroin, and 3 parts to 10 parts of oryzanol; The particle size of the silk fibroin emulsion is within 320.74 nm.

2. A silk fibroin emulsion containing oryzanol, characterized in that: The silk fibroin emulsion is composed of the following components in parts by mass: 575 parts to 633 parts of water, 350 parts to 400 parts of camellia oil, 12 parts to 15 parts of silk fibroin, and 5 parts to 10 parts of oryzanol; The particle size of the silk fibroin emulsion is within 300.03 nm.

3. A silk fibroin emulsion containing oryzanol, characterized in that: The silk fibroin emulsion is composed of the following components in parts by mass: 522 parts of water, 450 parts of camellia oil, 18 parts of silk fibroin, and 10 parts of oryzanol; The particle size of the silk fibroin emulsion is 305.36 nm.

4. Use of the silk fibroin emulsion containing oryzanol according to any one of claims 1 to 3 in cosmetic raw materials.

5. Use of the silk fibroin emulsion containing oryzanol according to any one of claims 1 to 3 in the raw materials of any product such as lotion, facial mask, lotion or cream.

Images