Hydrogel repair method for preserving bone and soft tissue defects by using alveolar ridge and application of hydrogel repair method

Abstract

The invention discloses a hydrogel repair method using alveolar crest to preserve bone and soft tissue defects and application thereof, and relates to the technical field of alveolar crest bone and soft tissue defects, high-molecular polymers are used as raw materials, the purity of the raw materials needs to reach 99% or above, raw material powder is dissolved in a PBS (phosphate buffer solution) according to the mass ratio of 10%, and the raw material powder is mixed with a water solution to prepare a hydrogel solution for repairing the alveolar crest bone and soft tissue defects. In the dissolving process, a magnetic stirrer needs to be used for stirring at room temperature (25 DEG C) for at least 4 hours until the solution is completely transparent and has no visible particles or blocks; the stirring speed is controlled to be 300-500 rpm, so that excessive foam is prevented from being generated. The dental implant has the advantage of efficient biocompatibility, in the actual using process, along with rapid development of biological material science, novel materials such as injectable hydrogel bring revolutionary changes for repairing of alveolar ridge bone and soft tissue defects, and the materials have good biocompatibility, degradability and certain mechanical strength, and can be used for repairing of the alveolar ridge bone and soft tissue defects. The microenvironment of natural bone tissues can be simulated.

Description

Technical Field

[0001] The invention relates to the technical field of alveolar ridge bone and soft tissue defects, and in particular to a method for repairing alveolar ridge preserved bone and soft tissue defects with hydrogel and an application thereof. Background Art

[0002] Alveolar ridge bone and soft tissue defects are common problems in stomatology, involving the loss or damage of bones (alveolar ridge bones) and soft tissues (such as gums). This situation may be caused by a variety of reasons, including periodontal disease, trauma, poor healing of the alveolar socket after tooth extraction, etc. In short, alveolar ridge bone and soft tissue defects are the incomplete or missing state of bone tissue and surrounding soft tissue in the oral cavity, and appropriate restorative measures need to be taken to restore its function and aesthetics.

[0003] The alveolar ridge is a key structure that supports teeth and maintains oral function. Its defects not only affect the appearance, but may also lead to decreased chewing function, pronunciation disorders and increased psychological stress. Therefore, it is particularly important to find an efficient and low-invasive repair method.

[0004] Traditionally, doctors often use surgical methods such as autologous bone transplantation or allogeneic bone transplantation to repair such defects. Although autologous bone transplantation can provide good biocompatibility and bone induction ability, the surgical process is complicated and involves secondary trauma, which increases the patient's pain and recovery time. At the same time, the source of autologous bone is limited, and it is particularly difficult to repair large defects. Although allogeneic bone transplantation can solve the problem of material source, the resulting immune rejection reaction and disease transmission risk limit its application to a certain extent.

[0005] In view of the above problems, the present invention provides a method for repairing alveolar ridge-preserved bone and soft tissue defects using hydrogel and its application. Summary of the invention

[0006] The purpose of the present invention is to provide a method for repairing alveolar ridge bone and soft tissue defects with hydrogel and its application. In actual use, with the rapid development of biomaterials science, new materials such as injectable hydrogels have brought revolutionary changes to the repair of alveolar ridge bone and soft tissue defects. Such materials have good biocompatibility, degradability and certain mechanical strength, can simulate the microenvironment of natural bone tissue, promote cell adhesion, proliferation and differentiation, thereby accelerating the regeneration and repair of bone tissue. More importantly, the use of injectable hydrogels greatly simplifies the surgical process, reduces trauma, reduces the risk of postoperative infection, and helps to shorten the patient's recovery period; specifically, the injectable hydrogel can be directly injected into the defect site in a minimally invasive manner to fill and support the defect area, provide a scaffold and nutrition for the growth of bone cells, and at the same time, it can load active substances such as growth factors and drugs inside to further promote the regeneration and repair of bone tissue. This treatment method not only improves the repair effect, but also reduces the pain and psychological burden of patients, and opens up a new way for the repair of alveolar ridge bone and soft tissue defects, thereby solving the problems in the background technology.

[0007] To achieve the above object, the present invention provides the following technical solution: a method for repairing alveolar ridge preserved bone and soft tissue defects with hydrogel, the method comprising the following steps:

[0008] S1: Use high molecular polymer as raw material with a purity of more than 99%. Dissolve the raw material powder in PBS (phosphate buffered saline) buffer at a mass ratio of 10%. Use a magnetic stirrer to stir at room temperature (25°C) for at least 4 hours until the solution is completely transparent with no visible particles or lumps. Control the stirring speed at 300-500rpm to avoid excessive foaming.

[0009] S2: Through the chemical cross-linking method, Ru(bpy)3C l2 was selected as a visible light initiator with a concentration of 1% (w / v) based on the mass of the polymer. Under light-proof conditions, the initiator was added to the solution and mixed evenly, so that the polymer formed a hydrogel with a three-dimensional network structure. Specifically, the photoinitiator was activated under 405nm near-blue light irradiation, and the light source irradiation was 15 minutes to ensure that the hydrogel was fully cross-linked, thereby generating free radicals or cations, which then triggered efficient cross-linking of the hydrogel. Its microstructure and mechanical properties were easier to regulate, and the controllability of the cross-linking kinetics was better. Ethylene glycol chitosan hydrogel was prepared by selecting a visible light initiator for encapsulating bone morphogenetic protein-2 (BMP-2) and transforming growth factor-β1, which promoted the regeneration of bone defects. Ru and sodium persulfate (SPS) were used as visible light initiators to synthesize a methacryloyl gelatin hydrogel (Gel MA), which has better cell compatibility than ultraviolet light cross-linking to ensure that the hydrogel has suitable mechanical properties and biodegradability.

[0010] S3: The hydrogel is purified to remove unreacted raw materials and impurities, and then strictly sterilized to ensure the sterility of the hydrogel.

[0011] S4: According to the surgical plan, prepare growth factors and drugs. Growth factors such as BMP-2 (bone morphogenetic protein-2) and TGF-β (transforming growth factor-β) with a concentration of 100 ng / mL can promote the regeneration of bone and soft tissue. Drugs such as antibiotics, such as gentamicin with a concentration of 1 mg / mL, can prevent postoperative infection.

[0012] S5: The growth factors and drugs are dissolved in a solution, and then the dissolved solution is loaded into the hydrogel. The loading method can be direct mixing, adsorption, or chemical coupling. The loading conditions need to be controlled during the loading process. BMP-2 and gentamicin are dissolved in PBS and mixed evenly. The mixed solution is slowly dripped into the hydrogel powder while stirring to ensure uniform mixing. After loading is completed, a brief centrifugation (5000 rpm, 5 minutes) is performed again to remove excess solution, and a sterility test is performed. Allogeneic bone material can be added to provide support for the hydrogel and promote the formation of new bone, ultimately ensuring that the growth factors and drugs can be continuously released and exert their effects in vivo.

[0013] Furthermore, in step S1, the polymer is selected from one of gelatin, collagen, chitosan, hyaluronic acid, and sodium alginate.

[0014] Furthermore, in step S3, purification generally comprises the following steps:

[0015] S3.1: Place the prepared hydrogel in a dialysis bag, soak it in a large amount of deionized water, change the water every 4 hours, and dialyze for 25 hours. Through dialysis, small molecule impurities and unreacted raw materials can be removed.

[0016] S3.2: For hydrogels containing larger particle impurities, centrifugation at 10,000 rpm for 10 minutes was repeated twice. Through high-speed centrifugation, the impurities can be separated from the hydrogel, thereby obtaining a purer hydrogel.

[0017] S3.3: Filtering the hydrogel solution using a microporous filter membrane with a pore size of 0.22 μm can further remove tiny particles and impurities suspended in the solution.

[0018] S3.4: For hydrogels that need to be preserved for a long time, freeze-drying can be used for purification. The purified hydrogel solution can be freeze-dried. The freeze-drying temperature is set to -50°C and the vacuum degree is maintained below 10Pa until the water is completely sublimated to obtain dry hydrogel powder. The original structure and properties of the hydrogel can also be maintained.

[0019] Furthermore, in step S3, the disinfection is intended to kill microorganisms (such as bacteria and viruses) in the hydrogel to ensure sterility, wherein the disinfection method is selected from ultraviolet irradiation, gamma ray irradiation, chemical disinfection and moist heat sterilization;

[0020] Ultraviolet irradiation: The bactericidal effect of ultraviolet rays is used to disinfect the hydrogel. Ultraviolet rays can destroy the DNA structure of microorganisms, thereby achieving the purpose of sterilization.

[0021] Gamma ray irradiation: Gamma rays are a type of high-energy electromagnetic wave with strong penetrating power and bactericidal ability. Through gamma ray irradiation, microorganisms in the hydrogel can be completely killed, including some difficult-to-kill spores and viruses.

[0022] Chemical disinfection method: Use chemical disinfectants (such as ethanol, hydrogen peroxide) to disinfect the hydrogel. Chemical disinfectants can destroy the cell walls and cell membranes of microorganisms, thereby achieving the purpose of sterilization.

[0023] Moist heat sterilization: The hydrogel is placed in a high temperature and high pressure environment to kill microorganisms through the action of moist heat.

[0024] Further, in step S5, the loading conditions are: temperature, pH value, solvent selection, loading method, and aseptic operation;

[0025] Temperature: The loading temperature was controlled at 4°C to maintain the activity of growth factors and drugs.

[0026] pH value: Adjust the solution pH to 7.4, which is close to the human body environment, to ensure the activity of growth factors and drugs.

[0027] Solvent selection: PBS buffer was selected as the solvent to ensure the solubility and stability of growth factors and drugs.

[0028] Loading methods: Loading methods include physical adsorption, chemical binding, and microencapsulation. By controlling the reaction time and temperature, the growth factors and drugs are evenly distributed in the hydrogel.

[0029] Aseptic operation: Aseptic operation needs to be maintained during the loading process to prevent contamination by microorganisms such as bacteria and fungi. This can be achieved by operating in a sterile room, using sterile equipment and sterilized solutions.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The present invention provides a method for repairing alveolar ridge bone and soft tissue defects with hydrogel and its application, which have the advantages of high efficiency and biocompatibility. In actual use, with the rapid development of biomaterials science, new materials such as injectable hydrogels have brought revolutionary changes to the repair of alveolar ridge bone and soft tissue defects. Such materials have good biocompatibility, degradability and certain mechanical strength, can simulate the microenvironment of natural bone tissue, promote cell adhesion, proliferation and differentiation, thereby accelerating the regeneration and repair of bone tissue. More importantly, the use of injectable hydrogels greatly simplifies the surgical process, reduces trauma, reduces the risk of postoperative infection, and helps to shorten the patient's recovery period; specifically, the injectable hydrogel can be directly injected into the defect site in a minimally invasive manner to fill and support the defect area, provide a scaffold and nutrition for the growth of bone cells, and at the same time, it can be loaded with active substances such as growth factors and drugs to further promote the regeneration and repair of bone tissue. This treatment method not only improves the repair effect, but also reduces the pain and psychological burden of patients, and opens up a new way for the repair of alveolar ridge bone and soft tissue defects. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The present invention is a flow chart of the preparation method. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In order to solve the technical problems, the following preferred technical solutions are provided:

[0035] A method for repairing alveolar ridge-preserving bone and soft tissue defects with hydrogel, the method comprising the following steps:

[0036] S1: Use high molecular polymer as raw material with a purity of more than 99%. Dissolve the raw material powder in PBS phosphate buffer at a mass ratio of 10%. During the dissolution process, use a magnetic stirrer to stir at room temperature 25°C for at least 4 hours until the solution is completely transparent with no visible particles or lumps. Control the stirring speed at 300-500rpm to avoid excessive foaming.

[0037] Specifically, in step S1, the polymer is selected from one of gelatin, collagen, chitosan, hyaluronic acid, and sodium alginate.

[0038] Gelatin is a natural protein formed by hydrolysis of triple-helix collagen into single-chain molecules. It has high biocompatibility and biodegradability. A minimally invasive injectable hydrogel ink is prepared using four-arm polyethylene glycol (PEG), oxidized dextran and amino gelatin through a two-step manufacturing process. Encapsulated preosteoblasts are loaded into the hydrogel to achieve diffusion and proliferation. It has sufficient mechanical properties, good biocompatibility and biodegradability. Amphiphilic poly (N-isopropylacrylamide) is grafted onto the gelatin molecule to prepare a macroporous hydrogel with dual thermal responsiveness, which helps to promote the formation of a high internal phase emulsion and can enhance the proliferation and penetration ability of fibroblasts during cell implantation.

[0039] Hyaluronic acid exists in the extracellular matrix of adult cartilage. It is a disaccharide unit composed of N-acetylglucosamine and glucuronic acid. Due to its excellent biocompatibility, it is considered an ideal cartilage repair biomaterial.

[0040] Collagen is widely present in the skin, bones, cartilage, connective tissue and ligaments of mammals. It is mainly composed of amino acid sequence, has high biocompatibility, and can be enzymatically degraded by collagenase. Collagen hydrogel scaffolds have the advantages of thermal reversibility, high biocompatibility and cell compatibility. Minimally invasive injectable collagen hydrogels are prepared by mixing type I and type II collagen. Changing the content of type I collagen in the hydrogel can adjust the compression modulus of the scaffold. The chondrocytes embedded in it can maintain their natural morphology in the hydrogel and secrete cartilage-specific extracellular matrix. The hydrogel mixed with type I collagen (95%) and type III collagen (5%) shows very excellent injection performance and repair performance.

[0041] Chitosan is a linear polysaccharide extracted from natural chitin, known for its low toxicity and high biocompatibility. Due to its structure similar to natural glycosaminoglycans in cartilage, it is increasingly used in cartilage repair applications. Chitosan tough hydrogel is prepared by in situ precipitation method. The hydrogel shows good biodegradability, biocompatibility and sufficient mechanical properties in application. Hydroxyethyl cellulose is used as a cross-linking agent to combine glycerol phosphate with chitosan to prepare chitosan-based injectable hydrogel, which achieves the regeneration and repair of cartilage tissue.

[0042] Alginate is an anionic polysaccharide extracted from the cell wall of brown algae. It is mainly used in drug delivery research involving wound healing. Injectable alginate hydrogels are prepared using alginate. In order to improve the cell adhesion ability of alginate, it can also be modified by mixing other polymers.

[0043] S2: Through the chemical cross-linking method, Ru(bpy)3C l2 was selected as a visible light initiator with a concentration of 1% w / v based on the mass of the polymer. Under light-proof conditions, the initiator was added to the solution and mixed evenly, so that the polymer formed a hydrogel with a three-dimensional network structure. Specifically, the photoinitiator was activated under 405nm near-blue light irradiation, and the light source irradiation was 15 minutes to ensure that the hydrogel was fully cross-linked, thereby generating free radicals or cations, which then triggered efficient cross-linking of the hydrogel. Its microstructure and mechanical properties were easier to regulate, and the controllability of the cross-linking kinetics was better. Ethylene glycol chitosan hydrogel was prepared by selecting a visible light initiator for encapsulating bone morphogenetic protein-2 (BMP-2) and transforming growth factor-β1, which promoted the regeneration of bone defects. Ru and sodium persulfate (SPS) were used as visible light initiators to synthesize a methacryloyl gelatin hydrogel (Gel MA), which has better cell compatibility than ultraviolet light cross-linking to ensure that the hydrogel has suitable mechanical properties and biodegradability.

[0044] S3: The hydrogel is purified to remove unreacted raw materials and impurities, and then strictly sterilized to ensure the sterility of the hydrogel.

[0045] Specifically, in step S3, purification generally comprises the following steps:

[0046] S3.1: Place the prepared hydrogel in a dialysis bag, soak it in a large amount of deionized water, change the water every 4 hours, and dialyze for 25 hours. Through dialysis, small molecule impurities and unreacted raw materials can be removed.

[0047] S3.2: For hydrogels containing larger particle impurities, centrifugation at 10,000 rpm for 10 minutes was repeated twice. Through high-speed centrifugation, the impurities can be separated from the hydrogel, thereby obtaining a purer hydrogel.

[0048] S3.3: Filtering the hydrogel solution using a microporous filter membrane with a pore size of 0.22 μm can further remove tiny particles and impurities suspended in the solution.

[0049] S3.4: For hydrogels that need to be preserved for a long time, freeze-drying can be used for purification. The purified hydrogel solution can be freeze-dried. The freeze-drying temperature is set to -50°C and the vacuum degree is maintained below 10Pa until the water is completely sublimated to obtain dry hydrogel powder. The original structure and properties of the hydrogel can also be maintained.

[0050] Further, in step S3, the disinfection is aimed at killing microorganisms such as bacteria and viruses in the hydrogel to ensure sterility, wherein the disinfection method is selected from ultraviolet irradiation, gamma ray irradiation, chemical disinfection and moist heat sterilization;

[0051] Ultraviolet irradiation: The bactericidal effect of ultraviolet rays is used to disinfect the hydrogel. Ultraviolet rays can destroy the DNA structure of microorganisms, thereby achieving the purpose of sterilization.

[0052] Gamma ray irradiation: Gamma rays are a type of high-energy electromagnetic wave with strong penetrating power and bactericidal ability. Through gamma ray irradiation, microorganisms in the hydrogel can be completely killed, including some difficult-to-kill spores and viruses.

[0053] Chemical disinfection method: Use chemical disinfectants such as ethanol and hydrogen peroxide to disinfect the hydrogel. Chemical disinfectants can destroy the cell walls and cell membranes of microorganisms, thereby achieving the purpose of sterilization.

[0054] Moist heat sterilization: The hydrogel is placed in a high temperature and high pressure environment to kill microorganisms through the action of moist heat.

[0055] S4: According to the surgical plan, prepare growth factors and drugs. Growth factors such as BMP-2 bone morphogenetic protein-2 and TGF-β transforming growth factor-β, with a concentration of 100 ng / mL, can promote the regeneration of bone and soft tissue. Drugs such as antibiotics, such as gentamicin, with a concentration of 1 mg / mL, can prevent postoperative infection.

[0056] S5: The growth factors and drugs are dissolved in a solution, and then the dissolved solution is loaded into the hydrogel. The loading method can be direct mixing, adsorption, or chemical coupling. The loading conditions need to be controlled during the loading process. BMP-2 and gentamicin are dissolved in PBS and mixed evenly. The mixed solution is slowly dripped into the hydrogel powder while stirring to ensure uniform mixing. After loading is completed, a brief centrifugation at 5000 rpm for 5 minutes is performed again to remove excess solution, and a sterility test is performed. Allogeneic bone material can be added to provide support for the hydrogel and promote the formation of new bone, ultimately ensuring that the growth factors and drugs can be continuously released and exert their effects in vivo.

[0057] Specifically, in step S5, the loading conditions are: temperature, pH value, solvent selection, loading method, and aseptic operation;

[0058] Temperature: The loading temperature was controlled at 4°C to maintain the activity of growth factors and drugs.

[0059] pH value: Adjust the solution pH to 7.4, which is close to the human body environment, to ensure the activity of growth factors and drugs.

[0060] Solvent selection: PBS buffer was selected as the solvent to ensure the solubility and stability of growth factors and drugs.

[0061] Loading methods: Loading methods include physical adsorption, chemical binding, and microencapsulation. By controlling the reaction time and temperature, the growth factors and drugs are evenly distributed in the hydrogel.

[0062] Aseptic operation: Aseptic operation needs to be maintained during the loading process to prevent contamination by microorganisms such as bacteria and fungi. This can be achieved by operating in a sterile room, using sterile equipment and sterilized solutions.

[0063] Application of hydrogel repair method

[0064] Minimally invasive injection: Injectable hydrogel is directly injected into the alveolar ridge bone and soft tissue defect area in a minimally invasive manner to fill and support the defect area, reducing surgical trauma and postoperative recovery time.

[0065] Promote cell adhesion and proliferation: Hydrogel materials can simulate the microenvironment of natural bone tissue, promote cell adhesion, proliferation and differentiation, and provide the necessary cellular basis for the regeneration of the defect area.

[0066] Accelerate bone tissue regeneration: By loading active substances such as growth factors, hydrogel materials can accelerate the regeneration process of bone tissue and improve the repair effect and quality.

[0067] Soft tissue repair: Hydrogel materials are also suitable for the repair of soft tissue defects. They can promote the healing and regeneration of soft tissue and restore the beauty and function of the oral cavity.

[0068] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0069] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for repairing alveolar ridge preservation bone and soft tissue defects with hydrogel, characterized in that: The method comprises the following steps: S1: Use high molecular polymer as raw material with a purity of more than 99%. Dissolve the raw material powder in PBS (phosphate buffered saline) buffer at a mass ratio of 10%. Use a magnetic stirrer to stir at room temperature (25°C) for at least 4 hours until the solution is completely transparent with no visible particles or lumps. Control the stirring speed at 300-500rpm to avoid excessive foaming. S2: Ru(bpy)3Cl2 was selected as a visible light initiator by chemical crosslinking, with a concentration of 1% (w / v) based on the mass of the polymer, and the initiator was added to the solution under light-proof conditions and mixed evenly, so that the polymer formed a hydrogel with a three-dimensional network structure. Specifically, the photoinitiator was activated under 405nm near-blue light irradiation, and the light source irradiation was 15 minutes to ensure that the hydrogel was fully crosslinked, thereby generating free radicals or cations, and then initiating efficient crosslinking of the hydrogel. Its microstructure and mechanical properties were easier to regulate, and the controllability of the crosslinking kinetics was better. Ethylene glycol chitosan hydrogel was prepared by selecting a visible light initiator for encapsulating bone morphogenetic protein-2 (BMP-2) and transforming growth factor-β1, which promoted the regeneration of bone defects. Ru and sodium persulfate (SPS) were used as visible light initiators to synthesize a methacryloyl gelatin hydrogel (GelMA), which has better cell compatibility than ultraviolet light crosslinking to ensure that the hydrogel has suitable mechanical properties and biodegradability. S3: Purify the hydrogel to remove unreacted raw materials and impurities, and then perform strict disinfection to ensure the sterility of the hydrogel; S4: According to the surgical plan, prepare growth factors and drugs. Growth factors such as BMP-2 (bone morphogenetic protein-2) and TGF-β (transforming growth factor-β) with a concentration of 100 ng / mL can promote the regeneration of bone and soft tissue. Drugs such as antibiotics, such as gentamicin with a concentration of 1 mg / mL, can prevent postoperative infection. S5: The growth factors and drugs are dissolved in a solution, and then the dissolved solution is loaded into the hydrogel. The loading method can be direct mixing, adsorption, or chemical coupling. The loading conditions need to be controlled during the loading process. BMP-2 and gentamicin are dissolved in PBS and mixed evenly. The mixed solution is slowly dripped into the hydrogel powder while stirring to ensure uniform mixing. After loading is completed, a brief centrifugation (5000 rpm, 5 minutes) is performed again to remove excess solution, and a sterility test is performed. Allogeneic bone material can be added to provide support for the hydrogel and promote the formation of new bone, ultimately ensuring that the growth factors and drugs can be continuously released and exert their effects in vivo.

2. A method for repairing alveolar ridge preservation bone and soft tissue defects using hydrogel according to claim 1, characterized in that: In step S1, the polymer is selected from one of gelatin, collagen, chitosan, hyaluronic acid, and sodium alginate.

3. The method for repairing alveolar ridge preservation bone and soft tissue defects with hydrogel according to claim 1, characterized in that: In step S3, purification generally comprises the following steps: S3.1: Place the prepared hydrogel in a dialysis bag, soak it in a large amount of deionized water, change the water every 4 hours, and dialyze for 25 hours. Through dialysis, small molecular impurities and unreacted raw materials can be removed; S3.2: For hydrogels containing larger particle impurities, centrifugation at 10,000 rpm for 10 minutes was repeated twice. The impurities can be separated from the hydrogel by high-speed centrifugation, thereby obtaining a purer hydrogel. S3.3: Filter the hydrogel solution using a microporous filter membrane with a pore size of 0.22 μm to further remove tiny particles and impurities suspended in the solution; S3.4: For hydrogels that need to be preserved for a long time, freeze-drying can be used for purification. The purified hydrogel solution can be freeze-dried. The freeze-drying temperature is set to -50°C and the vacuum degree is maintained below 10Pa until the water is completely sublimated to obtain dry hydrogel powder. The original structure and properties of the hydrogel can also be maintained.

4. The method for repairing alveolar ridge preservation bone and soft tissue defects with hydrogel according to claim 1, characterized in that: In step S3, the disinfection is intended to kill microorganisms (such as bacteria and viruses) in the hydrogel to ensure sterility, wherein the disinfection method is selected from ultraviolet irradiation, gamma ray irradiation, chemical disinfection and moist heat sterilization; Ultraviolet irradiation: The bactericidal effect of ultraviolet rays is used to disinfect the hydrogel. Ultraviolet rays can destroy the DNA structure of microorganisms, thereby achieving the purpose of sterilization; γ-ray irradiation: γ-ray is a kind of high-energy electromagnetic wave with strong penetrating power and bactericidal ability. Through γ-ray irradiation, microorganisms in hydrogels can be completely killed, including some difficult-to-kill spores and viruses; Chemical disinfection method: Use chemical disinfectants (such as ethanol, hydrogen peroxide) to disinfect the hydrogel. Chemical disinfectants can destroy the cell walls and cell membranes of microorganisms, thereby achieving the purpose of sterilization; Moist heat sterilization: The hydrogel is placed in a high temperature and high pressure environment to kill microorganisms through the action of moist heat.

5. The method for repairing alveolar ridge preservation bone and soft tissue defects with hydrogel according to claim 1, characterized in that: In step S5, the loading conditions are: temperature, pH value, solvent selection, loading method, and aseptic operation; Temperature: The loading temperature was controlled at 4°C to maintain the activity of growth factors and drugs; pH value: Adjust the solution pH to 7.4, which is close to the human body environment, to ensure the activity of growth factors and drugs; Solvent selection: PBS buffer was selected as the solvent to ensure the solubility and stability of growth factors and drugs; Loading methods: Loading methods include physical adsorption, chemical binding, and microencapsulation. By controlling the reaction time and temperature, the growth factors and drugs are evenly distributed in the hydrogel; Aseptic operation: Aseptic operation needs to be maintained during the loading process to prevent contamination by microorganisms such as bacteria and fungi. This can be achieved by operating in a sterile room, using sterile equipment and sterilized solutions.

Images