Preparation method and application of compound microbial fertilizer

Abstract

The invention discloses a preparation method and application of a compound microbial fertilizer, and belongs to the technical field of fertilizers. The preparation method comprises the following steps: A, pouring the urea, the sodium borate, the magnesium carbonate, the monoammonium phosphate and the monopotassium phosphate into a reaction kettle, uniformly mixing by stirring, then adding the alginate oligosaccharide, the potassium sulfate and the complex microbial inoculant, heating at the temperature of 20-37 DEG C, and stirring for 50-80 minutes to form slurry; and B, feeding the produced slurry into a granulator, mixing with a returned material, carrying out drum granulation, and drying, cooling and screening the granulated particles to obtain the granular compound fertilizer. The compound microbial fertilizer is comprehensive in nutrition, beneficial to microorganism utilization, suitable for unmanned aerial vehicle fertilization, lasting in fertilizer efficiency, capable of promoting crop growth, inhibiting pathogenic bacteria and the like, is an efficient and environment-friendly compound fertilizer, and has a wide application prospect in modern agricultural production.

Description

Technical Field

[0001] The present invention belongs to the technical field of fertilizers, and in particular relates to a preparation method and application of a composite microbial fertilizer. Background Art

[0002] Compound fertilizer refers to a chemical fertilizer containing two or more major nutrient elements. It offers comprehensive nutrients, is easy to apply, and has long-lasting effects, making it an important fertilizer type in modern agricultural production.

[0003] At present, there are two main methods for producing compound fertilizers:

[0004] Acid method: the raw materials are added into the drum granulator, and then granulation, drying, cooling and other processes are carried out.

[0005] Melting method: melt the raw materials, and then carry out granulation, cooling and other processes.

[0006] The advantages of the ammonia method for producing compound fertilizers are simplicity and ease of operation, but the disadvantages are high energy consumption, high costs, and the generation of large amounts of dust during production, which can cause environmental pollution. The advantages of the melting method for producing compound fertilizers are that the process is environmentally friendly and reduces the generation of dust and waste gas, but the disadvantage is the high equipment investment.

[0007] To address the high energy consumption and dust generation issues of the amino acid method for producing compound fertilizers, various improvements have been proposed to optimize production processes and reduce environmental pollution. However, the melting method is not suitable for the application of granular fertilizers containing microbial agents.

[0008] For example, the existing patent, application number: CN201610338752.8, discloses a high-concentration inorganic composite microbial fertilizer, and its technical solution is as follows: "The high-concentration inorganic composite microbial fertilizer is characterized in that the raw material components are as follows by weight: 300-330 parts of urea, 350-370 parts of monoammonium phosphate, 280-320 parts of potassium chloride, 6-12 parts of humic acid, 1-5 parts of EDTA chelated zinc, 1-5 parts of EDTA chelated manganese, 0.1-1 part of ammonium molybdate, 1-6 parts of borax, 3-6 parts of anti-caking agent, and 1-5 parts of Bacillus bacterial agent; the above raw materials are all commercially available, wherein: the urea is small-particle urea with N ≥ 46.2% and a particle size of 0.85mm-2.80mm; the monoammonium phosphate is ordinary The patent states that the content of the raw materials is ≥60% in powder form, wherein the nitrogen content is ≥10% and the phosphorus pentoxide content is ≥50%; the potassium chloride is a white powder, wherein the potassium oxide content is ≥60%; the humic acid has a molecular weight of 2000-5000, is a brown-black powder, has a nitrogen content of 1%-6%, and a humic acid content of ≥60% on a dry basis; the EDTA chelated zinc is a white powder, with a chelated zinc content of ≥15%; the EDTA chelated manganese is a light red powder, with a chelated manganese content of ≥13%; the ammonium molybdate is a white powder, with a molybdenum content of ≥54%; the boric acid is a white powder, with a boron content of ≥71%; the Bacillus microbial agent is a gray powder, with an effective viable count of ≥100 billion / gram; and the particle size of the above raw materials, except urea, is within the range of 0-1 mm. However, this patent is not suitable for this application. Most Bacillus microbial agents are hazardous, and the overall components are complex and not suitable for large-scale promotion and use. In addition, the lack of components that can lower the pH value of the humic acid makes it difficult to plant crops such as corn. Summary of the Invention

[0009] 1. Problems to be solved

[0010] In response to the problems existing in the above-mentioned prior art, the present invention provides a preparation method and application of a composite microbial fertilizer. The composite microbial fertilizer of the present invention has comprehensive nutrition, is conducive to microbial utilization, is suitable for drone fertilization, has long-lasting fertilizer effect, promotes crop growth, inhibits pathogens, etc. It is an efficient and environmentally friendly compound fertilizer with broad application prospects in modern agricultural production.

[0011] 2. Technical solution

[0012] To solve the above problems, the present invention adopts the following technical solutions.

[0013] A method for preparing a composite microbial fertilizer comprises the following steps:

[0014] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 20°C-37°C and stir for 50-80 minutes to form a slurry.

[0015] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0016] Preferably, in step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0017] 80-140 parts of urea,

[0018] 2-10 parts of sodium borate,

[0019] 10-15 parts of magnesium carbonate,

[0020] 6-20 parts of monoammonium phosphate,

[0021] 7-20 parts of potassium dihydrogen phosphate,

[0022] 10-20 parts of brown algae oligosaccharide,

[0023] 6-8 parts of potassium sulfate,

[0024] 2-6 parts of compound bacterial agent.

[0025] Preferably, in step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0026] 90-120 parts of urea,

[0027] 2-7 parts of sodium borate,

[0028] 10-13 parts of magnesium carbonate,

[0029] 6-10 parts of monoammonium phosphate,

[0030] 12-20 parts of potassium dihydrogen phosphate,

[0031] 10-20 parts of brown algae oligosaccharide,

[0032] 6-8 parts of potassium sulfate,

[0033] 2-6 parts of compound bacterial agent.

[0034] Preferably, in step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0035] 110 parts of urea,

[0036] 5 parts of sodium borate,

[0037] 12 parts of magnesium carbonate,

[0038] 8 parts of monoammonium phosphate,

[0039] 16 parts of potassium dihydrogen phosphate,

[0040] 15 parts of brown algae oligosaccharide,

[0041] 7 parts of potassium sulfate,

[0042] 4 parts of compound bacterial agent.

[0043] Preferably, the composite bacterial agent is a freeze-dried powder;

[0044] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio between the Rhizopus oryzae and the Pseudomonas stutzeri is (2-5): (3-6).

[0045] Preferably, the Rhizopus oryzae is purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0046] Preferably, the Pseudomonas stutzeri was deposited in the General Microbiology Center of China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0047] Preferably, in step B, the temperature of the drying process is 50-60° C., and the moisture content after drying is less than 30%.

[0048] Preferably, in step B, the temperature of the cooling process is 20-30°C;

[0049] In step B, the particle size of the sieved particles is 2 mm.

[0050] A composite microbial fertilizer is used in corn fertilization.

[0051] 3. Beneficial effects

[0052] Compared with the prior art, the present invention has the following beneficial effects:

[0053] The composite microbial fertilizer of the present invention has the following advantages: comprehensive nutrition, not only containing macroelements such as nitrogen, phosphorus, and potassium, but also adding a large number of trace elements, which makes up for the deficiency of organic matter in the fertilizer and makes the nutrients more comprehensive; it is conducive to the comprehensive utilization of the composite bacterial agent, and the synergistic effect of Rhizopus oryzae and Pseudomonas stutzeri can improve the effectiveness of nutrients; when applied to drone fertilization technology, it can be quickly decomposed when it falls on the soil surface, so that the powdered organic fertilizer penetrates into the soil with water, thereby improving the utilization efficiency of the fertilizer; the fertilizer effect is long-lasting, and the addition of slow-release organic matter and microorganisms has a long-lasting fertilizer effect, which can continuously improve the physical, chemical and biological properties of the soil; it promotes crop growth, provides comprehensive nutritional elements, and meets the needs of crop growth; Rhizopus oryzae and Pseudomonas stutzeri can secrete plant growth-promoting substances, improve soil structure, promote root development, and enhance the stress resistance of crops; it inhibits pathogens, and Pseudomonas stutzeri has the function of inhibiting pathogens, improves the soil environment, and is not conducive to the growth of pathogens. In summary, the composite microbial fertilizer of the present invention has comprehensive nutrition, is conducive to microbial utilization, is suitable for drone fertilization, has long-lasting fertilizer effect, promotes crop growth, inhibits pathogens, etc. It is an efficient and environmentally friendly composite fertilizer with broad application prospects in modern agricultural production. DETAILED DESCRIPTION

[0054] The present invention will be further described below with reference to specific embodiments.

[0055] Example 1

[0056] The preparation method of the composite microbial fertilizer comprises the following steps:

[0057] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 20°C and stir for 80 minutes to form a slurry.

[0058] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0059] The preparation method of the composite microbial fertilizer described above,

[0060] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0061] 80 parts of urea,

[0062] 10 parts of sodium borate,

[0063] 10 parts of magnesium carbonate,

[0064] 20 parts of monoammonium phosphate,

[0065] 7 parts of potassium dihydrogen phosphate,

[0066] 20 parts of brown algae oligosaccharide,

[0067] 6 parts of potassium sulfate,

[0068] 6 parts of compound bacterial agent.

[0069] The preparation method of the composite microbial fertilizer described above,

[0070] The composite bacterial agent is freeze-dried powder;

[0071] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 2:6.

[0072] The preparation method of the composite microbial fertilizer described above,

[0073] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0074] The preparation method of the composite microbial fertilizer described above,

[0075] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0076] The preparation method of the composite microbial fertilizer described above,

[0077] In step B, the drying temperature is 50° C., and the moisture content after drying is less than 30%.

[0078] The preparation method of the composite microbial fertilizer described above,

[0079] In step B, the temperature of the cooling process is 20°C;

[0080] In step B, the particle size of the sieved particles is 2 mm.

[0081] Example 2

[0082] The preparation method of the composite microbial fertilizer comprises the following steps:

[0083] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 37°C and stir for 50 minutes to form a slurry.

[0084] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0085] The preparation method of the composite microbial fertilizer described above,

[0086] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0087] 140 parts of urea,

[0088] 2 parts of sodium borate,

[0089] 15 parts of magnesium carbonate,

[0090] 6 parts of monoammonium phosphate,

[0091] 20 parts of potassium dihydrogen phosphate,

[0092] 10 parts of brown algae oligosaccharide,

[0093] 8 parts of potassium sulfate,

[0094] 2 parts of compound bacterial agent.

[0095] The preparation method of the composite microbial fertilizer described above,

[0096] The composite bacterial agent is freeze-dried powder;

[0097] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 5:3.

[0098] The preparation method of the composite microbial fertilizer described above,

[0099] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0100] The preparation method of the composite microbial fertilizer described above,

[0101] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0102] The preparation method of the composite microbial fertilizer described above,

[0103] In step B, the drying temperature is 60° C., and the moisture content after drying is less than 30%.

[0104] The preparation method of the composite microbial fertilizer described above,

[0105] In step B, the temperature of the cooling process is 20;

[0106] In step B, the particle size of the sieved particles is 2 mm.

[0107] Example 3

[0108] The preparation method of the composite microbial fertilizer comprises the following steps:

[0109] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 20°C and stir for 50 minutes to form a slurry.

[0110] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0111] The preparation method of the composite microbial fertilizer described above,

[0112] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0113] 90 parts of urea,

[0114] 7 parts of sodium borate,

[0115] 10 parts of magnesium carbonate,

[0116] 10 parts of monoammonium phosphate,

[0117] 12 parts of potassium dihydrogen phosphate,

[0118] 20 parts of brown algae oligosaccharide,

[0119] 6 parts of potassium sulfate,

[0120] 6 parts of compound bacterial agent.

[0121] The preparation method of the composite microbial fertilizer described above,

[0122] The composite bacterial agent is freeze-dried powder;

[0123] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 2:3.

[0124] The preparation method of the composite microbial fertilizer described above,

[0125] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0126] The preparation method of the composite microbial fertilizer described above,

[0127] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0128] The preparation method of the composite microbial fertilizer described above,

[0129] In step B, the drying temperature is 50° C., and the moisture content after drying is less than 30%.

[0130] The preparation method of the composite microbial fertilizer described above,

[0131] In step B, the temperature of the cooling process is 20°C;

[0132] In step B, the particle size of the sieved particles is 2 mm.

[0133] Example 4

[0134] The preparation method of the composite microbial fertilizer comprises the following steps:

[0135] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 37°C and stir for 80 minutes to form a slurry.

[0136] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0137] The preparation method of the composite microbial fertilizer described above,

[0138] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0139] 120 parts of urea,

[0140] 7 parts of sodium borate,

[0141] 13 parts of magnesium carbonate,

[0142] 10 parts of monoammonium phosphate,

[0143] 20 parts of potassium dihydrogen phosphate,

[0144] 20 parts of brown algae oligosaccharide,

[0145] 8 parts of potassium sulfate,

[0146] 6 parts of compound bacterial agent.

[0147] The preparation method of the composite microbial fertilizer described above,

[0148] The composite bacterial agent is freeze-dried powder;

[0149] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 5:6.

[0150] The preparation method of the composite microbial fertilizer described above,

[0151] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0152] The preparation method of the composite microbial fertilizer described above,

[0153] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0154] The preparation method of the composite microbial fertilizer described above,

[0155] In step B, the drying temperature is 60° C., and the moisture content after drying is less than 30%.

[0156] The preparation method of the composite microbial fertilizer described above,

[0157] In step B, the temperature of the cooling process is 30°C;

[0158] In step B, the particle size of the sieved particles is 2 mm.

[0159] Example 5

[0160] The preparation method of the composite microbial fertilizer comprises the following steps:

[0161] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 28°C and stir for 70 minutes to form a slurry.

[0162] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0163] The preparation method of the composite microbial fertilizer described above,

[0164] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0165] 110 parts of urea,

[0166] 5 parts of sodium borate,

[0167] 12 parts of magnesium carbonate,

[0168] 8 parts of monoammonium phosphate,

[0169] 16 parts of potassium dihydrogen phosphate,

[0170] 15 parts of brown algae oligosaccharide,

[0171] 7 parts of potassium sulfate,

[0172] 4 parts of compound bacterial agent.

[0173] The preparation method of the composite microbial fertilizer described above,

[0174] The composite bacterial agent is freeze-dried powder;

[0175] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 4:5.

[0176] The preparation method of the composite microbial fertilizer described above,

[0177] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0178] The preparation method of the composite microbial fertilizer described above,

[0179] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0180] The preparation method of the composite microbial fertilizer described above,

[0181] In step B, the drying temperature is 55° C., and the moisture content after drying is less than 30%.

[0182] The preparation method of the composite microbial fertilizer described above,

[0183] In step B, the temperature of the cooling process is 25°C;

[0184] In step B, the particle size of the sieved particles is 2 mm.

[0185] Comparative Example 1

[0186] The preparation method of the composite microbial fertilizer comprises the following steps:

[0187] A. Pour urea, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 28°C and stir for 70 minutes to form a slurry.

[0188] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0189] The preparation method of the composite microbial fertilizer described above,

[0190] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0191] 110 parts of urea,

[0192] 12 parts of magnesium carbonate,

[0193] 8 parts of monoammonium phosphate,

[0194] 16 parts of potassium dihydrogen phosphate,

[0195] 15 parts of brown algae oligosaccharide,

[0196] 7 parts of potassium sulfate,

[0197] 4 parts of compound bacterial agent.

[0198] The preparation method of the composite microbial fertilizer described above,

[0199] The composite bacterial agent is freeze-dried powder;

[0200] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 4:5.

[0201] The preparation method of the composite microbial fertilizer described above,

[0202] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0203] The preparation method of the composite microbial fertilizer described above,

[0204] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0205] The preparation method of the composite microbial fertilizer described above,

[0206] In step B, the drying temperature is 55° C., and the moisture content after drying is less than 30%.

[0207] The preparation method of the composite microbial fertilizer described above,

[0208] In step B, the temperature of the cooling process is 25°C;

[0209] In step B, the particle size of the sieved particles is 2 mm.

[0210] Comparative Example 2

[0211] The preparation method of the composite microbial fertilizer comprises the following steps:

[0212] A. Pour urea, sodium borate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and a composite bacterial agent. Heat to 28°C and stir for 70 minutes to form a slurry.

[0213] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0214] The preparation method of the composite microbial fertilizer described above,

[0215] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0216] 110 parts of urea,

[0217] 5 parts of sodium borate,

[0218] 8 parts of monoammonium phosphate,

[0219] 16 parts of potassium dihydrogen phosphate,

[0220] 15 parts of brown algae oligosaccharide,

[0221] 7 parts of potassium sulfate,

[0222] 4 parts of compound bacterial agent.

[0223] The preparation method of the composite microbial fertilizer described above,

[0224] The composite bacterial agent is freeze-dried powder;

[0225] The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is 4:5.

[0226] The preparation method of the composite microbial fertilizer described above,

[0227] The Rhizopus oryzae was purchased from Beijing Beinachuanglian Biotechnology Research Institute, wherein the strain number of Rhizopus oryzae is ATCC 10404.

[0228] The preparation method of the composite microbial fertilizer described above,

[0229] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0230] The preparation method of the composite microbial fertilizer described above,

[0231] In step B, the drying temperature is 55° C., and the moisture content after drying is less than 30%.

[0232] The preparation method of the composite microbial fertilizer described above,

[0233] In step B, the temperature of the cooling process is 25°C;

[0234] In step B, the particle size of the sieved particles is 2 mm.

[0235] Comparative Example 3

[0236] The preparation method of the composite microbial fertilizer comprises the following steps:

[0237] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and composite bacterial agent. Heat to 28°C and stir for 70 minutes to form a slurry.

[0238] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0239] The preparation method of the composite microbial fertilizer described above,

[0240] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0241] 110 parts of urea,

[0242] 5 parts of sodium borate,

[0243] 12 parts of magnesium carbonate,

[0244] 8 parts of monoammonium phosphate,

[0245] 16 parts of potassium dihydrogen phosphate,

[0246] 15 parts of brown algae oligosaccharide,

[0247] 7 parts of potassium sulfate,

[0248] 4 parts of compound bacterial agent.

[0249] The preparation method of the composite microbial fertilizer described above,

[0250] The composite bacterial agent is freeze-dried powder;

[0251] The composite bacterial agent is a mixture of Lactobacillus buchneri and Pseudomonas stutzeri, and the mass ratio of the Lactobacillus buchneri to the Pseudomonas stutzeri is 4:5.

[0252] The preparation method of the composite microbial fertilizer described above,

[0253] The Lactobacillus buchneri was deposited in the General Microbiology Center of the China Culture Collection Administration on January 15, 2015, with the deposit number 1.15607.

[0254] The preparation method of the composite microbial fertilizer described above,

[0255] The Pseudomonas stutzeri was deposited in the General Microbiology Center of the China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0256] The preparation method of the composite microbial fertilizer described above,

[0257] In step B, the drying temperature is 55° C., and the moisture content after drying is less than 30%.

[0258] The preparation method of the composite microbial fertilizer described above,

[0259] In step B, the temperature of the cooling process is 25°C;

[0260] In step B, the particle size of the sieved particles is 2 mm.

[0261] Comparative Example 4

[0262] The preparation method of the composite microbial fertilizer comprises the following steps:

[0263] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and bacterial agent. Heat to 28°C and stir for 70 minutes to form a slurry.

[0264] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0265] The preparation method of the composite microbial fertilizer described above,

[0266] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0267] 110 parts of urea,

[0268] 5 parts of sodium borate,

[0269] 12 parts of magnesium carbonate,

[0270] 8 parts of monoammonium phosphate,

[0271] 16 parts of potassium dihydrogen phosphate,

[0272] 15 parts of brown algae oligosaccharide,

[0273] 7 parts of potassium sulfate,

[0274] 4 parts of bacterial agent.

[0275] The preparation method of the composite microbial fertilizer described above,

[0276] The bacterial agent is freeze-dried powder;

[0277] The bacterial agent is Rhizopus oryzae, which is purchased from Beijing Beinachuanglian Biotechnology Research Institute, and the strain number of Rhizopus oryzae is ATCC 10404.

[0278] The preparation method of the composite microbial fertilizer described above,

[0279] In step B, the drying temperature is 55° C., and the moisture content after drying is less than 30%.

[0280] The preparation method of the composite microbial fertilizer described above,

[0281] In step B, the temperature of the cooling process is 25°C;

[0282] In step B, the particle size of the sieved particles is 2 mm.

[0283] Comparative Example 5

[0284] The preparation method of the composite microbial fertilizer comprises the following steps:

[0285] A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate, and potassium dihydrogen phosphate into a reactor and mix them evenly by stirring. Then add brown algae oligosaccharides, potassium sulfate, and bacterial agent. Heat to 28°C and stir for 70 minutes to form a slurry.

[0286] B. The slurry after production enters the granulator and is mixed with the return material and then granulated by drum. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

[0287] The preparation method of the composite microbial fertilizer described above,

[0288] In step A, the bacterial fertilizer is calculated in parts by weight, and the weight proportions of the raw materials are as follows:

[0289] 110 parts of urea,

[0290] 5 parts of sodium borate,

[0291] 12 parts of magnesium carbonate,

[0292] 8 parts of monoammonium phosphate,

[0293] 16 parts of potassium dihydrogen phosphate,

[0294] 15 parts of brown algae oligosaccharide,

[0295] 7 parts of potassium sulfate,

[0296] 4 parts of bacterial agent.

[0297] The preparation method of the composite microbial fertilizer described above,

[0298] The bacterial agent is freeze-dried powder;

[0299] The bacterial agent is Pseudomonas stutzeri, which was deposited in the General Microbiology Center of China Culture Collection Administration on April 2, 2015, with the deposit number 1.15198.

[0300] The preparation method of the composite microbial fertilizer described above,

[0301] In step B, the drying temperature is 55° C., and the moisture content after drying is less than 30%.

[0302] The preparation method of the composite microbial fertilizer described above,

[0303] In step B, the temperature of the cooling process is 25°C;

[0304] In step B, the particle size of the sieved particles is 2 mm.

[0305] Example 6

[0306] Specific application of bacterial fertilizer in Examples 1-5 and Comparative Examples 1-5:

[0307] We investigated the performance indicators of different preparation methods of composite microbial fertilizers, and the results are as follows (as shown in Table 1):

[0308] A Gaokexinnong M234-G solid material spreading drone (Example 5) was selected and applied to corn seedlings at a corn test base in Chaohu, Anhui Province. The above fertilizer was applied to corn seedlings in their early stages of growth. After 30 days, the growth status of the corn seedlings before and after application and the soil nutrient utilization rate (nitrogen, phosphorus, potassium content and organic matter content) were measured. Subsequently, an infection test for corn sheath blight was conducted during the flowering and fruiting period of corn, and the infection rate was calculated after 30 days.

[0309] Table 1

[0310]

[0311] The test shows (combined with Table 1) that, compared with Comparative Examples 1-5, the compound fertilizer spread in Examples 1-5, after 30 days, the average number of corn seedling leaves extracted is 8-10, the average root diameter of the corn seedlings is 10-13 cm, the average height of the corn seedlings is 30-39 cm, and the soil nutrient utilization rate is 41%-47%, which is significantly better than the corresponding situations in Comparative Examples 1-5. In addition, it can be seen from the average infection rate that the compound fertilizer spread in the embodiment of the present invention has a good antibacterial effect.

[0312] The preparation method of the composite microbial fertilizer in Comparative Example 1 differs from that in Example 5 in the following ways: sodium borate is not added; the amount of magnesium carbonate is slightly reduced; the amounts of monoammonium phosphate and potassium dihydrogen phosphate are reduced; the amount of brown algae oligosaccharide is slightly reduced; the amount of potassium sulfate is slightly reduced; and the amount of the composite microbial agent is slightly reduced. These differences affect the performance of the fertilizer. A specific analysis is as follows: No sodium borate is added: Boron is a trace element essential for plant growth, and its deficiency can affect plant growth and development. Boron promotes root development and improves plant resistance. Not adding sodium borate reduces the trace element content of the fertilizer, which is detrimental to plant growth. The amount of magnesium carbonate is slightly reduced: Magnesium carbonate is both a magnesium fertilizer and a soil conditioner. It can increase soil pH, improve acidic soils, and enhance soil fertility and drought resistance. Reducing the amount of magnesium carbonate reduces the fertilizer's soil-improving ability. Reduced amounts of monoammonium phosphate and potassium dihydrogen phosphate: Monoammonium phosphate and potassium dihydrogen phosphate are phosphorus fertilizers that promote plant rooting and increase yield. Reducing their amounts reduces the fertilizer's phosphorus fertilizer effectiveness. The dosage of brown algae oligosaccharide is slightly reduced: brown algae oligosaccharide has the effect of promoting plant growth and improving stress resistance. The reduction in dosage will reduce the performance of these functions. The dosage of potassium sulfate is slightly reduced: potassium sulfate is a potassium fertilizer that can increase the yield and quality of crops. The reduction in dosage will reduce the potassium fertilizer effect of the microbial fertilizer. The dosage of compound microbial agent is slightly reduced. Compound microbial agent is a key component of microbial fertilizer and mainly includes Rhizopus oryzae and Pseudomonas stutzeri. Rhizopus oryzae has the function of solubilizing phosphorus and potassium and activating trace elements in the soil. Pseudomonas stutzeri has the function of promoting plant growth and inhibiting pathogens. The reduction in the dosage of compound microbial agent will reduce the performance of these functions and affect the overall effect of the microbial fertilizer. In summary, the reduction in the dosage of each component in Comparative Example 1 will reduce the trace element content, soil improvement ability, phosphorus and potassium fertilizer effect, plant growth promotion and pathogen inhibition function of the microbial fertilizer, thereby affecting the overall performance of the microbial fertilizer.

[0313] The preparation method of the composite microbial fertilizer in Comparative Example 2 differs from that in Example 5 in the following ways: magnesium carbonate is not added; the dosages of monoammonium phosphate and potassium dihydrogen phosphate are reduced; the dosage of brown algae oligosaccharides is slightly reduced; the dosage of potassium sulfate is slightly reduced; and the dosage of the composite bacterial agent is slightly reduced. These differences affect the performance of the fertilizer, as analyzed below: No magnesium carbonate is added: Magnesium carbonate is both a magnesium fertilizer and a soil conditioner. It can increase soil pH, improve acidic soils, and enhance soil fertility and drought resistance. Not adding magnesium carbonate reduces the fertilizer's soil-improving ability. Monoammonium phosphate and potassium dihydrogen phosphate dosages are reduced: Monoammonium phosphate and potassium dihydrogen phosphate are phosphate fertilizers that promote plant rooting and fruiting, increasing yield. Reducing the dosages will reduce the fertilizer's phosphate efficiency. The dosage of brown algae oligosaccharides is slightly reduced: Brown algae oligosaccharides promote plant growth and enhance stress resistance. Reducing the dosage will reduce the effectiveness of these functions. The dosage of potassium sulfate is slightly reduced: Potassium sulfate is a potash fertilizer that can increase crop yield and quality. The reduction in dosage will reduce the potassium fertilizer effect of the microbial fertilizer. The dosage of the composite microbial agent is slightly reduced: the composite microbial agent is a key component of the microbial fertilizer, mainly including Rhizopus oryzae and Pseudomonas stutzeri. Rhizopus oryzae has the function of solubilizing phosphorus and potassium and activating trace elements in the soil, and Pseudomonas stutzeri has the function of promoting plant growth and inhibiting pathogens. The reduction in the dosage of the composite microbial agent will reduce the performance of these functions and affect the overall effect of the microbial fertilizer. In summary, the reduction in the dosage of each component in Comparative Example 2 will reduce the ability of the microbial fertilizer to improve the soil, the phosphorus and potassium fertilizer effect, the function of promoting plant growth and inhibiting pathogens, thereby affecting the overall performance of the microbial fertilizer.

[0314] The preparation method of the composite microbial fertilizer in Comparative Example 3 differs from that in Example 5 in the following ways: magnesium carbonate is not added; Lactobacillus buchneri is used in the composite agent instead of Pseudomonas stutzeri; and the accession number of Lactobacillus buchneri is different from that of Pseudomonas stutzeri. These differences affect the performance of the fertilizer, as analyzed below: No magnesium carbonate is added: Magnesium carbonate is both a magnesium fertilizer and a soil conditioner. It can increase soil pH, improve acidic soils, and increase soil fertility and drought resistance. Not adding magnesium carbonate reduces the fertilizer's soil-improving ability. Lactobacillus buchneri is used in the composite agent instead of Pseudomonas stutzeri: Lactobacillus buchneri and Pseudomonas stutzeri are both common plant growth-promoting bacteria, but their mechanisms of action differ slightly: Lactobacillus buchneri primarily promotes plant growth by fixing nitrogen, solubilizing phosphate, and inhibiting pathogens. In addition to these effects, Pseudomonas stutzeri can also produce plant hormones, induce systemic resistance, and improve plant stress resistance. Using Lactobacillus buchneri reduces the fertilizer's stress resistance and antibacterial effects. The accession number of Lactobacillus buchneri is different from that of Pseudomonas stutzeri: there are genetic differences between different strains, resulting in different physiological characteristics and metabolites. Using strains with different accession numbers will affect the overall performance of the bacterial fertilizer. In summary, the fact that no magnesium carbonate was added in Comparative Example 3, Lactobacillus buchneri was used instead of Pseudomonas stutzeri, and the different accession numbers will reduce the soil improvement ability, stress resistance, antibacterial effect, etc. of the bacterial fertilizer, thereby affecting the overall performance of the bacterial fertilizer.

[0315] The preparation method of the composite microbial fertilizer in Comparative Example 4 is compared with that in Example 5, mainly having the following differences: Raw material composition: Sodium borate and magnesium carbonate are used in Comparative Example 4, while sodium borate is not used in Example 5. The composite bacterial agent in Comparative Example 4 is Rhizopus oryzae, while a mixture of Rhizopus oryzae and Pseudomonas stutzeri is used in Example 5. Mechanism analysis: The role of sodium borate: Boron is an important trace element required for plant growth, which can promote the root development of plants and improve the stress resistance of plants. Adding sodium borate in Comparative Example 4 will help to increase the trace element content of the soil and promote plant growth. The role of magnesium carbonate: Magnesium carbonate is not only a magnesium fertilizer, but also can improve the pH value of the soil, increase the fertility and drought resistance of the soil. The use of magnesium carbonate in Comparative Example 4 will help to improve the physical and chemical properties of the soil and enhance the disease resistance and stress resistance of crops. Selection of composite bacterial agent: The Rhizopus oryzae used in Comparative Example 4 is a beneficial microorganism with functions such as phosphorus and potassium solubilization, which can improve the effectiveness of nutrients in the soil. In contrast, the mixed bacterial agent (Rhizopus oryzae and Pseudomonas stutzeri) used in Example 5 has a stronger antibacterial effect and the ability to promote plant growth. The influence of the dosage of each component: the dosage of each component in Comparative Example 4 is compared with Example 5, resulting in a different overall effect of the bacterial fertilizer. Specifically, the dosage of major nutrients such as urea, monoammonium phosphate and potassium dihydrogen phosphate directly affects the fertilizer efficiency and nutritional support of the plant. The composite microbial fertilizer preparation method of Comparative Example 4 can enhance the trace elements of the soil and improve soil properties, thereby promoting plant growth by adding sodium borate and magnesium carbonate. However, the selection of the composite bacterial agent and the dosage of each component will also significantly affect the overall performance of the bacterial fertilizer. Taken together, appropriate combination of ingredients and dosage adjustment will help improve the fertilizer efficiency of the bacterial fertilizer and the stress resistance of the plant.

[0316] The preparation method of the composite microbial fertilizer in Comparative Example 5 is compared with that in Example 5, mainly having the following differences: the selection of composite bacterial agent: the composite bacterial agent in Comparative Example 5 is Pseudomonas stutzeri, while what is used in Example 5 is a mixture of Rhizopus oryzae and Pseudomonas stutzeri. The role of Pseudomonas stutzeri: Pseudomonas stutzeri is a bacterium that has the function of promoting plant growth and inhibiting pathogens. It promotes plant growth by producing plant growth regulators (such as auxins, cytokinins, etc.), and can also inhibit the growth of pathogenic microorganisms by competing and producing antibiotics. The use of this bacterium alone will affect its antibacterial effect and the effect of promoting plant growth. The influence of the amount of each component: in Comparative Example 5, the amount of major nutrients such as urea, monoammonium phosphate and potassium dihydrogen phosphate is relatively stable compared with Example 5, but the single use of the composite bacterial agent will reduce the overall fertilizer effect. The addition of Rhizopus oryzae can provide the function of solubilizing phosphorus and potassium, and enhance the effectiveness of nutrients in the soil. The lack of Rhizopus oryzae will result in poor nutrient release effect. Supplementation of trace elements: The addition of sodium borate and magnesium carbonate helps to increase the trace element content of the soil, promote plant growth and improve stress resistance. The dosage of these ingredients in Comparative Example 5 is similar to that in Example 5, which can maintain the good physical and chemical properties of the soil. Impact of the overall effect: The single use of the composite bacterial agent in Comparative Example 5 will affect the overall performance of the fertilizer, especially in terms of disease resistance and the effect of promoting plant growth. The diversity of the composite bacterial agent in Example 5 enables it to show better adaptability and effect under different environmental conditions. The composite microbial fertilizer preparation method of Comparative Example 5 has certain limitations in promoting plant growth and inhibiting pathogens by using Pseudomonas stutzeri as a composite bacterial agent. Although the dosage of other ingredients remains stable, the lack of Rhizopus oryzae leads to a decrease in nutrient release efficiency, thereby affecting the overall performance of the fertilizer. Overall, the diversity of the composite bacterial agent and the reasonable combination of each component are the key to improving the effect of the fertilizer.

[0317] The composite microbial fertilizer of the present invention has the following advantages: comprehensive nutrition, not only containing macroelements such as nitrogen, phosphorus, and potassium, but also adding a large number of trace elements, which makes up for the deficiency of organic matter in the fertilizer and makes the nutrients more comprehensive; it is conducive to the comprehensive utilization of the composite bacterial agent, and the synergistic effect of Rhizopus oryzae and Pseudomonas stutzeri can improve the effectiveness of nutrients; when applied to drone fertilization technology, it can be quickly decomposed when it falls on the soil surface, so that the powdered organic fertilizer penetrates into the soil with water, thereby improving the utilization efficiency of the fertilizer; the fertilizer effect is long-lasting, and the addition of slow-release organic matter and microorganisms has a long-lasting fertilizer effect, which can continuously improve the physical, chemical and biological properties of the soil; it promotes crop growth, provides comprehensive nutritional elements, and meets the needs of crop growth; Rhizopus oryzae and Pseudomonas stutzeri can secrete plant growth-promoting substances, improve soil structure, promote root development, and enhance the stress resistance of crops; it inhibits pathogens, and Pseudomonas stutzeri has the function of inhibiting pathogens, improves the soil environment, and is not conducive to the growth of pathogens. In summary, the composite microbial fertilizer of the present invention has the advantages of comprehensive nutrition, being conducive to microbial utilization, being suitable for drone fertilization, having long-lasting fertilizer effect, promoting crop growth, and inhibiting pathogens. It is an efficient and environmentally friendly composite fertilizer with broad application prospects in modern agricultural production.

[0318] The above content is a further detailed description of the present invention in conjunction with specific implementation methods. It cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, they can make several simple deductions or substitutions without departing from the concept of the present invention, which should be regarded as falling within the scope of protection determined by the claims submitted for the present invention.

Claims

1. A method for preparing a composite microbial fertilizer, characterized in that: The following steps are involved: A. Pour urea, sodium borate, magnesium carbonate, monoammonium phosphate and potassium dihydrogen phosphate into a reaction kettle, stir to mix them evenly, then add brown algae oligosaccharide, potassium sulfate and composite bacterial agent, heat to 20°C-37°C, stir for 50min-80min, and form a slurry; B. The slurry after production enters the granulator and is mixed with the return material and then drum granulated. The granulated particles are then dried, cooled and sieved to obtain granular compound fertilizer.

2. The method for preparing the composite microbial fertilizer according to claim 1, characterized in that: In step A, the bacterial fertilizer is calculated by weight, and the weight proportions of the raw materials are as follows: 80-140 parts of urea, Sodium borate 2-10 parts, 10-15 parts of magnesium carbonate, 6-20 parts of monoammonium phosphate, 7-20 parts of potassium dihydrogen phosphate, 10-20 parts of brown algae oligosaccharide, Potassium sulfate 6-8 parts, 2-6 parts of compound bacterial agent.

3. The method for preparing the composite microbial fertilizer according to claim 2, characterized in that: In step A, the bacterial fertilizer is calculated by weight, and the weight proportions of the raw materials are as follows: 90-120 parts of urea, Sodium borate 2-7 parts, 10-13 parts of magnesium carbonate, 6-10 parts of monoammonium phosphate, 12-20 parts of potassium dihydrogen phosphate, 10-20 parts of brown algae oligosaccharide, Potassium sulfate 6-8 parts, 2-6 parts of compound bacterial agent.

4. The method for preparing the composite microbial fertilizer according to claim 3, characterized in that: In step A, the bacterial fertilizer is calculated by weight, and the weight proportions of the raw materials are as follows: 110 parts of urea, 5 parts of sodium borate, 12 parts of magnesium carbonate, 8 parts of monoammonium phosphate, 16 parts of potassium dihydrogen phosphate, 15 parts of brown algae oligosaccharide, 7 parts of potassium sulfate, 4 parts of compound bacterial agent.

5. The method for preparing the composite microbial fertilizer according to claim 4, characterized in that: The composite bacterial agent is freeze-dried powder; The composite bacterial agent is a mixture of Rhizopus oryzae and Pseudomonas stutzeri, and the mass ratio of the Rhizopus oryzae to the Pseudomonas stutzeri is (2-5):(3-6).

6. The method for preparing the composite microbial fertilizer according to claim 5, characterized in that: The Rhizopus oryzae was purchased from Beijing Beina Chuanglian Biotechnology Research Institute, and the strain number of Rhizopus oryzae is ATCC10404.

7. The method for preparing the composite microbial fertilizer according to claim 6, characterized in that: The Pseudomonas stutzeri was deposited in the General Microbiology Center of China National Committee for the Preservation of Microbiological Cultures on April 2, 2015, with the deposit number 1.15198.

8. The method for preparing the composite microbial fertilizer according to claim 7, characterized in that: In step B, the temperature of the drying process is 50-60° C., and the moisture content after drying is less than 30%.

9. The method for preparing the composite microbial fertilizer according to claim 8, characterized in that: In step B, the temperature of the cooling process is 20-30°C; In step B, the particle size of the sieved particles is 2 mm.

10. A composite microbial fertilizer is used in corn fertilization.

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