Double-layer temperature control edible fungus strain storage equipment and operation method thereof

Abstract

The invention discloses double-layer temperature control edible fungus strain storage equipment and an operation method thereof.The equipment is composed of an outer-layer storage chamber and inner-layer storage equipment, and the outer-layer storage chamber is provided with a polyurethane heat preservation board wall, a heat preservation door, outer-layer temperature control equipment, an ultraviolet lamp, an illuminating lamp, movable humidity control equipment and a shoe cabinet; the outer-layer storage room is divided into a storage room and a buffer room by partitions, the polyurethane heat preservation board walls are arranged on the top and peripheral walls of the outer-layer storage room, the outer-layer temperature control equipment is an air conditioner or a refrigerating unit, and ultraviolet lamps and illuminating lamps are uniformly mounted in the storage room and the buffer room; the movable humidity control equipment is arranged in the storage room A, the shoe cabinet is placed in a partition mode, and the inner-layer storage equipment is composed of a refrigerating box or a refrigerating cabinet or a refrigerator and placed in a storage equipment area of the storage room. Through internal and external double-layer temperature control, the storage environment conditions of the edible fungus strains are improved, and the storage effect is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus strain storage, and in particular relates to double-layer temperature-controlled edible fungus strain storage equipment and an operating method thereof. Background Art

[0002] Edible fungi strains refer to solid mycelial cultures growing on suitable substrates, including mother strains, original strains and cultivated strains. Unlike plant seeds whose life activities are basically stopped under normal conditions, edible fungi strains are actually the vegetative mycelium of edible fungi, which have vigorous life activities under normal conditions, and will age, autolyze and become inactive in a short period of time.

[0003] The preservation of edible fungi can be divided into preservation and storage according to the length of preservation time. The main purpose of edible fungi preservation is to maintain the viability of the fungi for a long time and maintain the genetic, morphological and physiological stability of the fungi. The preservation methods of edible fungi can be divided into mycelium preservation method and spore (the true seeds of edible fungi) preservation method according to different preservation materials. Mycelium preservation method is the most commonly used in production. Common ones include regular transplantation method, mineral oil sealing method, mycelium liquid preservation method, liquid nitrogen ultra-low temperature freezing method, natural matrix preservation method (such as sawdust preservation of fungi, manure preservation of mushroom fungi, wood block preservation method, branch preservation method, wheat grain preservation method, etc.), glycerol low temperature preservation method, etc. The spore preservation method mainly includes sand tube preservation method, basidiospore filter paper preservation method, and freeze vacuum drying method. It should be pointed out that after a long period of preservation, the fungi cannot be directly used in scientific research and production. Specific technology is required to revive the fungi before they can be used in scientific research and production. The purpose of edible fungus strain storage is to keep the physiological function of the strain from declining through suitable environmental conditions after the strain is produced and not used in a short period of time, so that it can still be used normally as a strain after a certain period of time. Since the objects of strain storage are normal edible fungi strains of all levels, and they are still used as production strains after storage, most of the strain preservation methods, such as mineral oil sealing method, mycelium liquid preservation method, liquid nitrogen ultra-low temperature freezing method, natural matrix preservation method, glycerol low temperature preservation method and spore preservation method, are not suitable for strain storage.

[0004] Production practice shows that the core technology of strain storage is to build storage conditions with constant temperature, constant humidity, cleanliness, light avoidance, and convenient retrieval. Most of the existing technologies are technologies that improve one of the above aspects through equipment innovation. CN202221150796.5 discloses a drawer-type edible fungus strain storage box, including a rectangular box body, the side of the box body is open, at least one pair of slides is arranged on the inner wall of the box body, drawers are correspondingly installed on the slides, label slots are arranged on the drawers, handles are arranged on the drawers, and each box surface of the box body and the inner and outer surfaces of the drawers are provided with a plurality of air holes. CN202223064283.5 provides a classification storage box for preserving edible fungi, comprising a box body with open front and rear ends, the side walls of the box body are evenly provided with a number of air holes, a placement plate that can be moved up and down is arranged between the left and right side walls of the box body, the upper end of the placement plate and the upper end of the inner bottom of the box body are evenly provided with a plurality of test tube storage chambers that can be moved forward and backward, the upper end of the placement plate and the upper end of the inner bottom of the box body are located behind the plurality of test tube storage chambers, and a plurality of U-shaped blocking net frames are correspondingly arranged around the outer side of the rear end of the test tube storage chamber, the front end of the test tube storage chamber is provided with a transparent label slot, and the lower end of the box body is evenly and symmetrically provided with a plurality of height-adjustable support legs. Both CN202221150796.5 and CN202223064283.5 have made innovative improvements to the bacterial strain storage equipment, but both have the following three shortcomings: (1) When taking out the seeds, the temperature of the remaining bacterial strains will change suddenly (especially obvious in high temperature seasons), which will also have adverse effects on the physiological and biochemical activities of the bacterial strains, causing the bacterial strains to spit out yellow water, brown, and even self-lyse, significantly shortening the storage effect of the bacterial strains. (2) When taking out the seeds, a large amount of condensed water will form on the outer wall of the remaining bacterial strains (the outer wall of the test tube, the outer wall of the bacterial strain bottle / bag) and the surface of the box body and drawer. The condensed water is easy to breed miscellaneous bacteria, thereby contaminating the bacterial strains. (3) Increase the rate of miscellaneous bacteria contamination during the storage of bacterial strains: There are two main reasons for the miscellaneous bacteria contamination during the storage of bacterial strains in this scheme: first, the condensed water formed on the outer wall of the bacterial strains (the outer wall of the test tube, the outer wall of the bacterial strain bottle / bag) and the surface of the box body breeds miscellaneous bacteria, producing spores to contaminate the bacterial strains; second, the environmental miscellaneous bacteria contaminate the bacterial strains when taking out the seeds. First, the surface of the box body of this scheme will increase the amount of condensed water, thereby increasing the amount of bacteria breeding caused by condensed water, and further increasing the bacteria contamination rate during the storage of the strains; secondly, the air holes of the box body have a certain interception effect on environmental bacteria, thereby increasing the bacteria contamination rate during the storage of the strains.CN202320888974.2 provides a device for preserving edible fungi strains, comprising an insulation box, the insulation box having an opening, the insulation box having a sealing cover threadedly connected thereto for sealing the opening, a plurality of fixed seats fixedly connected therein, a fixed block arranged between adjacent fixed seats, the fixed block being fixedly connected to the insulation box, a sleeve being detachably connected therein, a limiting rod for limiting the sleeve being inserted into the insulation box, a buffer pad being arranged between the fixed seat and the insulation box, a plurality of ventilation holes A being arranged therein, the plurality of ventilation holes A being symmetrically arranged on both sides of the insulation box, a plurality of ventilation holes B being arranged on the fixed seat, each ventilation hole B being coaxially arranged with a group of ventilation holes A on the insulation box, and a sealing plug for sealing the ventilation holes A and / or ventilation holes A being arranged on the insulation box. CN202311234402.3 discloses a classification storage box for preserving edible fungi strains, comprising a box body and two movable cavities, both of which are slidably connected to the box body; two placement plates are fixedly connected in each of the movable cavities, and a fixing component is installed on the placement plate, and the fixing component comprises an air collecting chamber, an air bag, a second spring, a cross plate, and a placement bottle, wherein the placement plate is provided with a circular groove in an equidistant array, the placement bottle is slidably connected in the circular groove, the air collecting chamber is fixedly connected to one side of the placement plate, the lower end of the air bag is fixedly connected to the air collecting chamber, the cross plate is fixedly connected to the upper end of the air bag and is slidably connected to the placement bottle, a plurality of the second springs are fixedly connected between the cross plate and the air collecting chamber, and a fourth spring is fixedly connected in the circular groove; a sliding rod is slidably connected to one side wall of the box body, an end of the sliding rod away from the box body is fixedly connected to an elliptical block, and a first spring is fixedly connected between the elliptical block and the box body. The devices provided by CN202320888974.2 and CN202311234402.3 have made further innovative improvements to the storage devices, but there are still problems such as a sudden change in temperature of the remaining strains when taking out the seeds, affecting the physiological and biochemical activities of the strains, and condensation water on the outer wall when taking out the seeds, breeding miscellaneous bacteria and contaminating the strains. Summary of the invention

[0005] In view of the above technical problems, the present invention provides a double-layer temperature-controlled edible fungus strain storage device and an operation method thereof, which improves the storage environment conditions of edible fungus strains and improves the storage effect through double-layer temperature control.

[0006] The technical solution of the present invention is as follows: A double-layer temperature-controlled edible fungus strain storage device consists of an outer storage room and an inner storage device. The outer storage room is provided with a polyurethane insulation board wall, an insulation door, an outer temperature control device, an ultraviolet lamp, a lighting lamp, a mobile humidity control device and a shoe cabinet, and the outer storage room is divided into a storage room and a buffer room by a partition. The polyurethane insulation board wall is arranged on the top and surrounding walls of the outer storage room, and the storage room and the buffer room are evenly installed with ultraviolet lamps and lighting lamps; the mobile humidity control device is arranged in the storage room, and the shoe cabinet is placed against the partition. The inner storage device consists of a refrigerator, a freezer or a refrigerator, and is placed in the storage equipment area of ​​the storage room.

[0007] Furthermore, the outer temperature control device is an air conditioner or a refrigeration unit, which can be selected according to actual needs.

[0008] Furthermore, the thickness of the polyurethane insulation board wall is 8-10 cm.

[0009] Furthermore, the thermal insulation door comprises two staggered thermal insulation doors, which can achieve the same thermal insulation effect as the thermal insulation wall.

[0010] Furthermore, the storage room and the buffer room are equipped with independent outer temperature control equipment or two temperature control ports are separated from the same outer temperature control equipment for separate temperature control. When storing straw mushrooms and black fungus that are not resistant to low temperatures, the temperature control equipment uses air conditioning, and the temperature of the temperature-controlled storage room and the buffer room is 14-18°C; when storing other low-temperature resistant edible fungi varieties other than straw mushrooms and black fungus, the temperature control equipment uses a refrigeration unit, and the temperature of the temperature-controlled storage room and the buffer room is 4-8°C.

[0011] Furthermore, the installation height of the ultraviolet lamp is 200±10 cm; the number of rows of ultraviolet lamps in the storage room is determined according to the width of the storage room. If the width of the storage room is less than or equal to 4 meters, one row of ultraviolet lamps is installed, and if the width is 4-6 meters, two rows of ultraviolet lamps are installed, that is, the vertical distance from the ultraviolet lamp to the nearest wall in the same direction should be less than or equal to 2 meters, and the ultraviolet lamps are evenly distributed in the storage room; one row of ultraviolet lamps is installed in the middle of the buffer room, and the number of ultraviolet lamps is determined according to the length of the buffer room. If it is less than or equal to 4 meters, one ultraviolet lamp is installed, and if it is 4-6 meters, two ultraviolet lamps are installed, that is, the vertical distance from the center point of the ultraviolet lamp to the nearest vertical wall should be less than or equal to 2 meters. The ultraviolet lamp is independently and automatically controlled by a programmable switch, and the light is turned on for disinfection and sterilization for 30-60 minutes when the bacteria are not taken in and put out (after get off work every day).

[0012] Furthermore, the light intensity of the lighting in the storage room is controlled at 300-600lx to meet the needs of taking and placing the strains, and should not be too strong. The light intensity of the lighting in the buffer room is controlled at 200-600lx to meet the needs of shoe changing operations, and should not be too strong.

[0013] Furthermore, the mobile humidity control equipment is a dehumidifier or a humidifier, which is equipped according to different regions and seasons. Dehumidifiers are equipped in high-humidity regions and seasons, and humidifiers are equipped in dry regions and seasons to control the relative humidity of the air in the storage room at 50%-60%.

[0014] Furthermore, the shoe cabinet has an open grid structure, and the size of the shoe cabinet is determined according to the number of people entering the culture storage room. Each person is assigned two adjacent grids, one for shoes entering the culture storage room and the other for outdoor shoes. The shoe cabinet is cleaned every 7-10 days.

[0015] Furthermore, the partition uses 4-6 cm thick polyurethane insulation board to completely separate the storage room and the buffer room. The storage room and the buffer room are kept clean, and the floor is mopped every 7-10 days. The mop is cleaned with bactericidal disinfectant (bleach solution, pasteurized disinfectant, etc.), wrung out and then mopped. The floor should be mopped before the ultraviolet lamp turns on automatically.

[0016] Furthermore, when storing low-temperature-intolerant varieties of straw mushrooms and black fungus, the temperature of the inner storage equipment is controlled at 13-15°C; when storing low-temperature-resistant edible fungi varieties other than straw mushrooms and black fungus, the temperature of the inner storage equipment is controlled at 3-5°C; the inner storage equipment is equipped with a backup power supply. When a power outage occurs, the backup power supply should be used in time to power the inner storage equipment and stop taking and placing the strains. The inner storage equipment stores strains in batches. When a batch of strains is used up, the storage rooms of each equipment are wiped and disinfected with 75% medical alcohol, and then put into use next time after drying.

[0017] The operation method of the above equipment is as follows: (1) Disinfection and sterilization before equipment startup

[0018] After the equipment is installed, first use a broom to thoroughly clean the inner wall of the outer storage room, the inner wall of the wall and the floor, then use a rag to wipe the inner wall of the outer storage room, the inner wall of the wall and the outer wall of the inner storage equipment, mop the floor with a sterilizing and disinfecting liquid (bleaching powder liquid, 84 disinfectant, etc.), wipe the inner wall of the inner storage equipment with a rag with medical alcohol (preferably 75% concentration), open the insulation door to disperse the residual alcohol, and then turn on the ultraviolet light of the outer storage room for 3-5 hours of disinfection; (2) Equipment startup

[0019] After the ultraviolet disinfection is completed, the operator changes into the shoes for entering the storage room in the buffer room, enters the storage room, closes the insulation door, and then starts the inner storage equipment. After the inner storage equipment reaches the set temperature, the outer temperature control equipment is started and the temperature of the storage room reaches the set temperature. (3) Place the bacteria in the

[0020] The cultured strains are labeled and transferred to the buffer room. The operator then changes into the shoes for entering the storage room in the buffer room, and then puts the strains into the inner storage equipment. The operation of putting in the strains should be quick, and then the equipment door should be closed quickly; (4) Extraction of bacterial strains

[0021] When taking out the strains, the operator changes into the shoes for entering the storage room in the buffer room, enters the storage room and takes out the strains from the inner storage equipment. The operation of taking out the strains should be quick, and then the equipment door should be closed quickly.

[0022] Furthermore, the daily management of the above equipment is: The UV lamp in the outer storage room is independently and automatically controlled by a programmable switch. The lamp is turned on for 30-60 minutes for disinfection when the bacteria are not being taken out (after get off work every day). The temperature control of the inner equipment is equipped with a backup power supply. When a power outage occurs, the backup power supply should be used in time to power the inner storage equipment and stop taking and placing the strains; Each inner storage device stores strains in batches. When a batch of strains is used up, it is closed and disinfected with medical alcohol (concentration of 75%). After drying, it is started up and put into next use. The floors of storage rooms and buffer rooms should be mopped every 7-10 days. The mop should be cleaned with bactericidal disinfectant (bleach solution, 84 disinfectant, etc.), wrung dry and then mopped. The floor should be mopped before the ultraviolet lamp turns on automatically. The shoe cabinet should be cleaned every 7-10 days before mopping the buffer room floor. The inner wall of the outer storage room roof, the inner wall of the wall and the outer wall of the inner storage equipment should be wiped with a rag every 1-2 months. Definitions of Abbreviations and Key Terms

[0023] Sudden temperature change: During the storage of bacterial strains, if the temperature changes by more than 10°C in a short period of time (5-10 minutes), a large amount of condensed water will form on the outer wall of the bacterial strain (outer wall of the test tube, outer wall of the bacterial strain bottle / battery) (condensed water is easy to breed miscellaneous bacteria), and it will also have an adverse effect on the physiological and biochemical activities of the bacterial strain, causing the bacterial strain to spit out yellow water, brown, or even autolyze, significantly shortening the storage effect of the bacterial strain.

[0024] The beneficial effects of the present invention are: (1) The present invention can prolong the storage time of bacterial strains: by controlling the temperature of the outer layer, it ensures that the temperature of the environment where the remaining bacterial strains are located is constant when the strains are taken, thereby avoiding sudden changes in the temperature of the bacterial strains, and further avoiding adverse effects on the physiological and biochemical activities of the bacterial strains. The storage period of the mother strain can be extended by 2 months, and the storage period of the original strain and the cultivated strain can be extended by 1 month.

[0025] (2) The present invention can prevent the formation of condensed water on the outer wall of the remaining bacteria (the outer wall of the test tube, the outer wall of the bacteria bottle / bag) when the bacteria are removed: by controlling the temperature and humidity of the outer layer, it can ensure that the temperature and humidity of the environment where the remaining bacteria are located are constant when the bacteria are removed, thereby preventing the formation of condensed water on the outer wall of the bacteria (the outer wall of the test tube, the outer wall of the bacteria bottle / bag), and further preventing the growth of miscellaneous bacteria on the outer wall of the bacteria.

[0026] (3) The present invention can reduce the contamination rate of bacteria during the storage of bacterial strains: There are two main reasons for the contamination of bacteria during the storage of bacterial strains. The first is that the condensed water formed on the outer wall of the bacterial strain (the outer wall of the test tube, the outer wall of the bacterial strain bottle / bag) breeds bacteria and produces spores to contaminate the bacterial strains; the second is that the environmental bacteria contaminate the bacterial strains when the strains are taken. By controlling the temperature and humidity of the outer layer to avoid the formation of condensed water on the outer wall of the bacterial strain (the outer wall of the test tube, the outer wall of the bacterial strain bottle / bag), the growth of bacteria caused by condensed water can be effectively eliminated; by regularly sterilizing and disinfecting the outer space, the outer environment can be kept clean and the amount of environmental bacteria can be reduced, thereby reducing the rate of bacterial contamination by bacteria by more than 70%. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a structural schematic diagram of the present invention, in which: 1, polyurethane insulation board wall, 201, insulation door I, 202, insulation door II, 301, outer temperature control equipment I, 302, outer temperature control equipment II, 401, ultraviolet lamp I, 402, ultraviolet lamp II, 403, ultraviolet lamp III, 404, ultraviolet lamp IV, 405, ultraviolet lamp V, 406, ultraviolet lamp VI, 407, ultraviolet lamp VII, 408, ultraviolet lamp VIII, 501, lighting lamp I, 502, lighting lamp II, 503, lighting lamp III, 6, mobile humidity control equipment, 7, shoe cabinet, 8, partition, A, storage room, B, buffer room, C, storage equipment area. DETAILED DESCRIPTION

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings, but the present invention is not limited thereto.

[0029] like Figure 1As shown, the present invention consists of an outer storage room and an inner storage device, the outer storage room is provided with a polyurethane insulation board wall 1, an insulation door I 201, an insulation door II 202, an outer temperature control device I 301, an outer temperature control device II 302, an ultraviolet lamp I 401, an ultraviolet lamp II 402, an ultraviolet lamp III 403, an ultraviolet lamp IV 404, an ultraviolet lamp V 405, an ultraviolet lamp VI 406, an ultraviolet lamp VII 407, an ultraviolet lamp VIII 408, an illuminating lamp I 501, an illuminating lamp II 502, an illuminating lamp III 503, mobile humidity control equipment 6 and shoe cabinet 7, and the outer storage room is divided into storage room A and buffer room B by partition 8, the inner storage equipment consists of a refrigerator, a freezer or a refrigerator, and is placed in the storage equipment area C of the storage room A, the thickness of the polyurethane insulation board wall 1 is 8-10 cm, and it is set on the top and surrounding walls of the outer storage room, the insulation door I 201 is set on the partition 8, and the insulation door II 202 is set on the wall of the outer storage room, and the two insulation doors are staggered. The outer temperature control equipment I 301 and the outer temperature control equipment II 302 can select air conditioning or refrigeration units according to actual needs to control the temperature of the outer storage room, and two rows of ultraviolet lamps are evenly installed in the storage room A, and ultraviolet lamps I 401, ultraviolet lamps II 402, and ultraviolet lamps III 403 are evenly installed in the same row, and ultraviolet lamps IV 404, ultraviolet lamps V405 and ultraviolet lamps VI 406 are evenly installed in another row, and a row of lighting lamps is installed between the two rows of UV lamps, and the lighting lamps are composed of lighting lamps I 501 and lighting lamps II 502; a row of UV lamps is evenly installed in buffer room B, including UV lamps VII 407 and UV lamps VIII 408, and another row is installed with a lighting lamp III 503, the mobile humidity control equipment 6 is arranged in the storage room A, the shoe cabinet 7 is placed against the partition 8, the shoe cabinet 7 is an open grid structure, the size of the shoe cabinet is determined according to the number of people entering the culture storage room, each person is allocated two adjacent grids, which respectively store the shoes entering the storage room and the outdoor shoes taken off, and the shoe cabinet is cleaned every 7-10 days; the inner storage equipment is arranged in the storage equipment area C, when storing the low-temperature-intolerant varieties of straw mushrooms and black fungus, the temperature of the inner storage equipment is controlled at 13-15°C; when storing other low-temperature-resistant edible fungi varieties except straw mushrooms and black fungus, the temperature of the inner storage equipment is controlled at 3-5°C; the inner storage equipment is equipped with a backup power supply. When a power outage occurs, the backup power supply should be used in time to power the inner storage equipment, and the taking and placing of the cultures should be stopped. The inner storage equipment stores the strains in batches. After a batch of strains is used up, the inner storage equipment is wiped and disinfected with 75% medical alcohol, and then put into next use after drying to prevent the strains from being contaminated by miscellaneous bacteria.

[0030] The specific operation method is as follows: (1) Disinfection and sterilization before equipment startup

[0031] After the equipment is installed, first use a broom to thoroughly clean the inner wall of the outer storage room, the inner wall of the wall and the floor, then use a rag to wipe the inner wall of the outer storage room, the inner wall of the wall and the outer wall of the inner storage equipment, mop the floor clean with 84 disinfectant, wipe the inner wall of the inner storage equipment with a 75% medical alcohol rag, open the equipment door to disperse the residual alcohol, and then turn on the ultraviolet light of the outer storage room for 3-5 hours of disinfection; (2) Equipment startup

[0032] After the ultraviolet disinfection is completed, the operator changes into the shoes for entering the storage room in the buffer room, enters the storage room, closes the insulation door, and then starts the inner storage equipment. After the inner storage equipment reaches the set temperature, the outer temperature control equipment is started and the temperature of the storage room reaches the set temperature. (3) Place the bacteria in the

[0033] The cultured strains are labeled and transferred to the buffer room. The operator then changes into the shoes for entering the storage room in the buffer room, and then puts the strains into the inner storage equipment in the storage room. The strains should be put in quickly, and then the equipment door should be closed quickly. (4) Extraction of bacterial strains

[0034] When taking out the strains, the operator changes into the shoes for entering the storage room in the buffer room, enters the storage room and takes out the strains from the inner storage equipment. The operation of taking out the strains should be quick, and then the equipment door should be closed quickly.

Claims

1. A double-layer temperature-controlled edible fungus strain storage device, characterized in that: It consists of an outer storage room and inner storage equipment. The outer storage room is provided with a polyurethane insulation board wall, an insulation door, an outer temperature control equipment, an ultraviolet lamp, a lighting lamp, a mobile humidity control equipment and a shoe cabinet, and the outer storage room is divided into a storage room and a buffer room by a partition. The polyurethane insulation board wall is arranged on the top and surrounding walls of the outer storage room. The storage room and the buffer room are evenly installed with ultraviolet lamps and lighting lamps; the mobile humidity control equipment is arranged in the storage room, the shoe cabinet is placed against the partition, and the inner storage equipment consists of a refrigerator, a freezer or a refrigerator, which is placed in the storage equipment area of ​​the storage room; the insulation door includes two staggered insulation doors; the storage room and the buffer room are installed with independent outer temperature control equipment or two temperature control ports are separated from the same outer temperature control equipment for separate temperature control.

2. The double-layer temperature-controlled edible fungus strain storage device according to claim 1 is characterized in that: The outer temperature control device is an air conditioner or a refrigeration unit.

3. The double-layer temperature-controlled edible fungus seed storage device according to claim 1 is characterized in that: The thickness of the polyurethane insulation board wall is 8-10 cm; the installation height of the ultraviolet lamp is 200±10 cm.

4. The double-layer temperature-controlled edible fungus seed storage device according to claim 1 is characterized in that: The illumination intensity of the lighting lamp in the storage room is controlled at 300-600lx, and the illumination intensity of the lighting lamp in the buffer room is controlled at 200-600lx.

5. The double-layer temperature-controlled edible fungus seed storage device according to claim 1 is characterized in that: The mobile humidity control equipment is a dehumidifier or a humidifier.

6. The double-layer temperature-controlled edible fungus seed storage device according to claim 1, characterized in that: The shoe cabinet is an open grid structure, and the size of the shoe cabinet is determined according to the number of people entering the culture storage room, and each person is allocated two adjacent grids.

7. The double-layer temperature-controlled edible fungus seed storage device according to claim 1, characterized in that: The partition adopts a polyurethane insulation board with a thickness of 4-6 cm.

8. The double-layer temperature-controlled edible fungus seed storage device according to claim 1, characterized in that: The inner storage equipment is equipped with a backup power supply.

9. The operating method of the double-layer temperature-controlled edible fungus seed storage device according to any one of claims 1 to 8, characterized in that: The steps include: (1) Disinfection and sterilization before equipment startup After the equipment is installed, first use a broom to thoroughly clean the inner wall, inner wall and floor of the outer storage room, then use a rag to wipe the inner wall, inner wall and outer wall of the inner storage equipment, mop the floor with a sterilizing disinfectant, wipe the inner wall of the inner storage equipment with a medical alcohol rag, open the insulation door to disperse the residual alcohol, and then turn on the ultraviolet light of the outer storage room for 3-5 hours; (2) Equipment startup After the ultraviolet disinfection is completed, the operator changes into the shoes for entering the storage room in the buffer room, enters the storage room, closes the insulation door, and then starts the inner storage equipment. After the inner storage equipment reaches the set temperature, the outer temperature control equipment is started and the temperature of the storage room reaches the set temperature. (3) Place the bacteria in the The cultured bacteria are labeled and transferred to the buffer room. The operator then changes into the shoes for entering the storage room in the buffer room and places the bacteria into the inner storage equipment. (4) Extraction of bacterial strains When taking out the strains, the operator changes into the shoes for entering the storage room in the buffer room, enters the storage room and takes out the strains from the inner storage equipment.

10. The operating method according to claim 9, characterized in that: The daily management of the double-layer temperature-controlled edible fungus strain storage equipment is as follows: The UV lamp in the outer storage room is independently and automatically controlled by a programmable switch. The light is turned on for 30-60 minutes for disinfection when the bacteria are not being taken out or placed. The temperature control of the inner equipment is equipped with a backup power supply. When a power outage occurs, the backup power supply is used to power the inner storage equipment and stop taking and placing the strains; Each inner storage device stores bacteria in batches. When a batch of bacteria is used up, it is closed and disinfected with medical alcohol. After drying, it is started up and put into next use. The floor of the storage room and buffer room should be mopped every 7-10 days. The mop should be cleaned with bactericidal disinfectant, wrung out and then mopped. The floor should be mopped before the ultraviolet lamp turns on automatically. The shoe cabinet should be cleaned every 7-10 days before mopping the buffer room floor. The inner wall of the outer storage room roof, the inner wall of the wall and the outer wall of the inner storage equipment should be wiped with a rag every 1-2 months.

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