Anti-aging and repairing cosmetic composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly discloses an anti-aging and repairing cosmetic composition as well as a preparation method and application thereof. The cosmetic composition is prepared from the following raw materials in parts by weight: 0.05 to 1.5 parts of an ergothioneine pine mushroom extract, 1 to 10 parts of oat firming protein, 0.01 to 1 part of Marilong eight-treasure raw powder, 0.001 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.000001 to 0.01 part of conopeptide, 0.00001 to 0.1 part of acetyl hexapeptide-8, 0.01 to 1 part of D-panthenol, 0.01 to 3 parts of nicotinamide and 0.01 to 1 part of sodium hyaluronate. The cosmetic composition provided by the invention simultaneously has multiple effects of immediate wrinkle removal, long-term anti-aging wrinkle removal, barrier repair and the like, the anti-aging repair effect of the product is greatly improved, and the maintenance process of consumers is effectively simplified. The prepared eye cream is small in irritation and good in anti-aging and repairing effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to an anti-aging and repairing cosmetic composition and a preparation method and application thereof. Background Art

[0002] In the overall survey of popular trends and consumer demand in the beauty industry, anti-aging, wrinkle removal and barrier repair have become the most frequently mentioned purchasing motivations by consumers. Currently, the anti-aging and wrinkle removal effects of cosmetics are divided into instant wrinkle removal and long-term anti-aging and wrinkle removal. Based on this, developing cosmetics that have multiple functions of instant wrinkle removal, long-term anti-aging and wrinkle removal, and barrier repair can effectively simplify consumers' maintenance process, better meet consumers' maintenance needs, and has great market prospects.

[0003] At present, the cosmetics on the market for anti-aging, wrinkle removal and barrier repair have relatively simple effects, because it is generally difficult to take into account instant wrinkle removal, long-term wrinkle removal and barrier repair. For example, patent application CN112043664A provides an anti-aging and wrinkle removal composition, including snake venom peptides, bee venom peptides, scorpion venom peptides, cono peptides, jellyfish extracts, and anti-free radical components, which have both instant wrinkle removal and long-term anti-aging effects; patent application CN113171319A provides an instant and long-term anti-aging and wrinkle removal composite plant extract, which is prepared from three plants: roselle, baobab fruit, and pomegranate fruit. It has significant anti-aging and wrinkle removal effects, takes effect immediately, can significantly remove wrinkles in 5 minutes, and shows excellent long-term anti-aging and wrinkle removal effects after long-term application; but the above anti-aging and wrinkle removal compositions do not have the effect of skin barrier repair, and the product effects are relatively simple. Patent application CN114886807A provides a composition with all-round skin barrier repair function, including velvetia velutipes extract, ectoine, and Centella asiatica extract. The three ingredients work synergistically to make the product have better skin barrier repair and instant anti-wrinkle effects, but it does not have long-lasting anti-aging and wrinkle removal effects, and fails to fully meet consumers' multi-dimensional maintenance needs.

[0004] Obviously, the existing products on the market cannot effectively meet the use needs of consumers. In view of this, the present invention is proposed. Summary of the invention

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide an anti-aging and repairing cosmetic composition and its preparation method and application, to provide a cosmetic composition that simultaneously has multiple functions such as instant wrinkle removal, long-term anti-aging and wrinkle removal, and barrier repair, thereby greatly improving the anti-aging and repairing effect of the product and effectively simplifying the consumer's maintenance process.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] In a first aspect, the present invention provides an anti-aging and repairing cosmetic composition, which is prepared from the following raw materials in parts by weight:

[0008]

[0009] In the above technical scheme, the provided composition contains a double peptide-cono peptide and acetyl hexapeptide-8; wherein, cono peptide is derived from a bionic polycyclic peptide of natural cono polypeptide, has 3 pairs of disulfide bonds, has super activity, can block ion channels, and thus selectively inhibit voltage-gated sodium channels; can reduce the efficiency of action potentials generated by excited cells, thereby reducing the efficiency of electrical signal propagation in nerves, helping to tighten the skin and relieve overly tense skin; acetyl hexapeptide-8 is a mimic of the N-terminal end of SNAP-25, participates in the competition for SNAP-25 sites in the melt bubble complex, thereby affecting the formation of the SNARE complex, achieving the effect of inhibiting expression lines and smoothing surface lines. The present invention significantly improves fibroblast activity and promotes collagen secretion through precise regulation of dual-target effects, achieving an immediate firming and wrinkle-removing effect.

[0010] In the provided composition, oat firming protein is a protein complex derived from the alpine plant oat, composed of proteins with a molecular weight of more than 5000 Daltons, which is in line with the molecular weight range of albumin. It has the effect of instantly tightening and removing wrinkles, making the skin feel instantly tight, and has a synergistic effect on film formation, moisturizing, and smoothing the skin.

[0011] In the provided composition, the ergothioneine Agaricus extract is prepared by co-fermentation of Hericium erinaceus and Agaricus matsutake, and is a naturally occurring antioxidant ingredient in the human body. It has the functions of inhibiting the expression of TNF-α and MMP-1 in human dermal fibroblasts caused by UV irradiation, regulating the activity of glutathione reductase and mitochondrial superoxide dismutase, inhibiting inflammation and DNA halogenation caused by UVB irradiation, reducing cell damage and death caused by UV irradiation, scavenging free radicals, reducing lipid peroxidation, absorbing ultraviolet light, and the like. In summary, the ergothioneine Agaricus extract has a strong effect of repairing the skin barrier and long-term anti-aging and wrinkle-removing effects.

[0012] In the provided composition, tetrahydromethylpyrimidine carboxylic acid, also known as ectoine, can protect cells from damage by ultraviolet rays, protect DNA from damage by ultraviolet rays and visible light, resist external pressure, improve cell stress capacity, protect Langerhans cells, enhance skin immune function, increase antioxidant enzyme activity, is highly hydrophilic, long-lastingly moisturizing, anti-inflammatory and soothing, repairs skin barrier, and can also enhance fibroblast activity and promote collagen synthesis, with good repair and anti-aging effects.

[0013] In the provided composition, zinc carbonate hydroxide in the Mayinglong Babao original powder has the effects of detoxification, improving eyesight, removing cataracts, absorbing moisture, relieving itching and healing sores, partially absorbing wound secretions, and inhibiting the growth of local staphylococci, and has good antiseptic, astringent and protective effects on skin inflammation and surface trauma; pearl has the effects of calming the mind, clearing away heat and nourishing yin, removing cataracts and improving eyesight, detoxifying and promoting tissue regeneration, and it also contains rich protein and trace elements, which can promote skin cell renewal, brighten skin color and reduce wrinkles; amber has the effects of calming nerves, dispersing blood stasis and stopping bleeding, treating carbuncle sores, traumatic injuries, and stopping bleeding and promoting tissue regeneration. Mayinglong Babao original powder has good effects of repairing skin barrier and long-term anti-aging and wrinkle removal.

[0014] In the provided composition, D-panthenol can improve the hydration of the stratum corneum, reduce TEWL, reduce skin roughness and soothe irritated skin; in addition, it can also activate fibroblast proliferation, accelerate epithelial cell regeneration and heal wounds.

[0015] In the provided composition, niacinamide (vitamin B3) is a very stable vitamin, which not only has good whitening effect, but also can enhance the skin barrier and fight aging; ceramide is an important component of the skin barrier, and niacinamide enhances the skin barrier function by increasing the ceramide content in the stratum corneum, reduces transepidermal water loss, and improves skin resistance; in addition, niacinamide can also promote the synthesis of epidermal protein, and long-term use of skin care products containing niacinamide can improve skin texture and significantly reduce fine lines and wrinkles.

[0016] In the provided composition, sodium hyaluronate can form a moisturizing film on the skin surface, effectively lock in moisture, increase the water content of the skin, and has a good moisturizing effect; it can also promote the proliferation and differentiation of epidermal cells, stimulate cell regeneration and scavenge oxygen free radicals, promote the repair of damaged tissues, and has a good skin barrier repair effect; small molecule hyaluronic acid can penetrate into the dermis, promote blood microcirculation, facilitate the skin's absorption of nutrients, and also has a good cosmetic and anti-wrinkle effect.

[0017] As the preferred amount mentioned above, the cosmetic composition is prepared from the following raw materials in parts by weight:

[0018]

[0019] As a further preferred embodiment of the above technical solution, the Mayinglong Babao raw powder is obtained by mixing zinc carbonate hydroxide, amber powder and pearl powder in a specific ratio by weight. Further, the Mayinglong Babao raw powder is obtained by mixing zinc carbonate hydroxide, amber powder and pearl powder in a mass ratio of 80-100: 0.1-2: 0.1-2; further, the mass ratio of zinc carbonate hydroxide, amber powder and pearl powder is 90-100: 0.1-1: 0.5-2.

[0020] In a second aspect, the present invention provides a use of the above cosmetic composition in preparing eye cream.

[0021] In a third aspect, the present invention provides an eye cream product based on the above cosmetic composition, which is specifically prepared from the cosmetic composition and auxiliary materials. It is understood that in order to improve sensory properties, provide skin nutrition, prevent quality deterioration, etc., various ingredients generally used in eye creams other than the above ingredients can be appropriately added to the composition of the present invention as needed within the scope that does not impair the effects of the present invention.

[0022] As a further preferred embodiment of the above technical solution, the auxiliary material is selected from one or more of emulsifiers, polyols, oils, thickeners, preservatives, pH regulators, fragrances, chelating agents, antioxidants, and purified water.

[0023] In the above technical solution, the emulsifier is selected from one or more of cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 stearate;

[0024] The polyol is selected from one or more of glycerol, 1,3-propylene glycol, and butylene glycol;

[0025] The oil is selected from one or more of dimethicone, dimethicone alcohol, cyclopentasiloxane, butylene glycol dicaprylate / dicaprate, hydrogenated polyisobutene, isopropyl myristate, caprylic / capric triglyceride, isononyl isononanoate, C12-15 alcohol benzoate, neopentyl glycol dicaprylate dicaprate, squalane, and avocado butter;

[0026] The thickener is selected from one or more of polyacrylamide, C13-14 isoparaffin, laureth-7, polyacrylate crosspolymer-6, acrylic acid (ester) / C10-30 alkyl acrylate crosspolymer, xanthan gum, hydroxyethyl cellulose, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, sorbitan isostearate, ammonium acryloyldimethyl taurate / VP copolymer, and sodium polyacryloyldimethyl taurate;

[0027] The pH regulator is selected from one or more of tartaric acid, citric acid, and malic acid;

[0028] The aromatic agent is selected from one or two of borneol and essence;

[0029] The chelating agent is selected from one or more of disodium EDTA and caprylhydroxamic acid;

[0030] The antioxidant is selected from one or more of butylated hydroxytoluene, butylated hydroxyanisole and tocopherol acetate.

[0031] The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, chlorphenesin, methylparaben, p-hydroxyacetophenone, propylparaben, 1,2-hexanediol, and sorbitan octanoate.

[0032] As a further preferred embodiment of the above technical solution, the dosage of each auxiliary material is as follows: 2 to 6 parts of emulsifier, 6 to 15 parts of polyol, 8 to 15 parts of oil, 0.1 to 1 part of thickener, 1 to 3 parts of preservative, 0.01 to 0.5 part of pH adjuster, 0.1 to 1 part of fragrance, 0.01 to 0.1 part of chelating agent, 1 to 5 parts of antioxidant, and 5 to 95 parts of purified water.

[0033] In a fourth aspect, the present invention further provides a method for preparing the above-mentioned eye cream, comprising the following steps:

[0034] S1. Add purified water, D-panthenol, niacinamide and chelating agent in phase A to an emulsifying tank, stir and heat, then add sodium hyaluronate and the first polyol in phase B, keep warm and stir, and homogenize in vacuum to obtain mixture a for later use;

[0035] S2, add the emulsifier, oil, antioxidant and first preservative in phase C to the pretreatment tank, heat and stir to mix evenly, then add the second polyol and Mayinglong Babao original powder in phase D, keep warm and stir to obtain mixture b, set aside;

[0036] S3, adding the mixture b obtained in step S2 to the mixture a obtained in step S1, stirring at a temperature of 100 °C to obtain a mixture c, which is set aside;

[0037] S4, adding the thickener and purified water in phase E to the mixture c obtained in step S3, homogenizing under vacuum, stirring and cooling to obtain a mixture d;

[0038] S5, adding the first aromatic agent and the third polyol in phase F to the mixture d obtained in step S4, homogenizing, stirring and cooling to obtain a mixture e;

[0039] S6, adding the ergothioneine Agrocybe tumefaciens extract, purified water, the second preservative, acetyl hexapeptide-8, cono peptide, oat firming protein in phase G, and the tetrahydromethylpyrimidine carboxylic acid and purified water in phase H to the mixture e obtained in step S5, homogenizing and stirring to obtain a mixture f;

[0040] S7, adding the pH adjuster and purified water in phase I and the third preservative and the second fragrance in phase J to the mixture f obtained in step S6, homogenizing and stirring, and after the treatment is completed, the eye cream is obtained.

[0041] As a further preferred embodiment of the above technical solution, in step S1, the temperature of heat preservation and stirring is 80-85°C;

[0042] In step S2, the temperature of heating and stirring is 80-85°C;

[0043] In step S3, the temperature of heat preservation and stirring is 80-85°C;

[0044] In step S4, the temperature is lowered to 50-55°C;

[0045] In step S5, the temperature is lowered to 40-45°C with stirring.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] (1) The cosmetic composition provided by the present invention utilizes the multi-target synergistic effect of dual peptides cono peptide and acetyl hexapeptide-8 with oat firming protein to significantly enhance fibroblast activity, promote collagen secretion, and make the skin feel instantly tight, thereby producing an instantly perceived wrinkle smoothing effect, achieving an instant firming and wrinkle removal effect within 10 minutes, and the instant wrinkle removal effect lasts for more than 120 minutes. At 120 minutes, the user can still clearly feel that there are fewer crow's feet than before and the skin is firmer.

[0048] (2) The cosmetic composition provided by the present invention utilizes the synergistic effect of ergothioneine, Agaricus truncatus extract, ectoine, Mayinglong Babao original powder, D-panthenol, niacinamide and sodium hyaluronate to significantly enhance the product's skin barrier repair and long-term anti-aging and wrinkle removal effects. Long-term use of the product prepared by the present invention can make the skin tougher and less wrinkled after 28 days, significantly improve the problem of skin sagging, effectively stimulate the synthesis of elastin and elastic fiber protein, and restore the skin to an elastic, firm state.

[0049] (3) The composition provided by the present invention has multiple functions such as instant wrinkle removal, long-term anti-aging and wrinkle removal, barrier repair, moisturizing, etc., which can effectively simplify the consumer's maintenance process and better meet the consumer's maintenance needs, and has a great market prospect.

[0050] In summary, the cosmetic composition provided by the present invention has high safety, low irritation, and is safe to use while meeting the short-term and long-term anti-aging and repair effects, and has excellent prospects for market promotion and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0051] Figure 1 The dynamic wrinkle effect pictures collected by the skin multifunctional 3D imager are from left to right before use, 10 minutes after use, and 120 minutes after use;

[0052] Figure 2 The static wrinkle effect pictures collected by the skin multifunctional 3D imager, from left to right are before use, after 14 days of use, and after 28 days of use. DETAILED DESCRIPTION

[0053] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0054] Unless otherwise specified, all commodities or reagents in this invention are purchased through market channels. Some raw material information is as follows:

[0055] The oatmeal skin-tightening protein used in the embodiments of the present invention was purchased from Beijing Dongfang Miaosen Biotechnology Co., Ltd.

[0056] The ergothioneine Agrocybe matsutake extract (trade name: BioyouthTM-EGT) used in the examples of the present invention was purchased from Bloomage Biotech Co., Ltd.

[0057] Example 1

[0058] An eye cream containing an anti-aging and repairing cosmetic composition is prepared from the following raw materials by weight:

[0059] Table 1 Example 1 Components

[0060]

[0061]

[0062] In this embodiment, a method for preparing the above eye cream is also provided, comprising the following steps:

[0063] S1. Add purified water, D-panthenol, niacinamide and chelating agent in phase A to an emulsifying tank, heat to 85° C., stir and heat, then add sodium hyaluronate and the first polyol in phase B, keep warm and stir, and homogenize in vacuum to obtain mixture a for later use;

[0064] S2, add the emulsifier, oil, antioxidant and first preservative in phase C to the pretreatment tank, heat to 85°C, heat and stir to mix evenly, then add the second polyol and Mayinglong Babao powder in phase D, keep warm and stir to obtain mixture b, set aside;

[0065] S3, adding the mixture b obtained in step S2 to the mixture a obtained in step S1, and stirring at 85°C to obtain a mixture c, which is set aside;

[0066] S4, adding the thickener and purified water in phase E to the mixture c obtained in step S3, homogenizing under vacuum, stirring and cooling to 55° C. to obtain a mixture d;

[0067] S5, adding the first aromatic agent and the third polyol in phase F to the mixture d obtained in step S4, homogenizing, stirring and cooling to 45° C. to obtain a mixture e;

[0068] S6, adding the ergothioneine Agrocybe tumefaciens extract, purified water, the second preservative, acetyl hexapeptide-8, cono peptide, oat firming protein in phase G, and the tetrahydromethylpyrimidine carboxylic acid and purified water in phase H to the mixture e obtained in step S5, homogenizing and stirring to obtain a mixture f;

[0069] S7, adding the pH adjuster and purified water in phase I and the third preservative and the second fragrance in phase J to the mixture f obtained in step S6, homogenizing and stirring, and after the treatment is completed, the eye cream is obtained.

[0070] Example 2

[0071] An eye cream containing an anti-aging and repairing cosmetic composition is prepared from the following raw materials by weight:

[0072] Table 2 Example 2 Components

[0073]

[0074]

[0075] In this embodiment, a method for preparing the above eye cream is also provided, comprising the following steps:

[0076] S1. Add purified water, D-panthenol, niacinamide and chelating agent in phase A to an emulsifying tank, heat to 85° C., stir and heat, then add sodium hyaluronate and the first polyol in phase B, keep warm and stir, and homogenize in vacuum to obtain mixture a for later use;

[0077] S2, add the emulsifier, oil, antioxidant and first preservative in phase C to the pretreatment tank, heat to 85°C, heat and stir to mix evenly, then add the second polyol and Mayinglong Babao powder in phase D, keep warm and stir to obtain mixture b, set aside;

[0078] S3, adding the mixture b obtained in step S2 to the mixture a obtained in step S1, and stirring at 85°C to obtain a mixture c, which is set aside;

[0079] S4, adding the thickener and purified water in phase E to the mixture c obtained in step S3, homogenizing under vacuum, stirring and cooling to 55° C. to obtain a mixture d;

[0080] S5, adding the first aromatic agent and the third polyol in phase F to the mixture d obtained in step S4, homogenizing, stirring and cooling to 45° C. to obtain a mixture e;

[0081] S6, adding the ergothioneine Agrocybe tumefaciens extract, purified water, the second preservative, acetyl hexapeptide-8, cono peptide, oat firming protein in phase G, and the tetrahydromethylpyrimidine carboxylic acid and purified water in phase H to the mixture e obtained in step S5, homogenizing and stirring to obtain a mixture f;

[0082] S7, adding the pH adjuster and purified water in phase I and the third preservative and the second fragrance in phase J to the mixture f obtained in step S6, homogenizing and stirring, and after the treatment is completed, the eye cream is obtained.

[0083] Example 3

[0084] An eye cream containing an anti-aging and repairing cosmetic composition is prepared from the following raw materials by weight:

[0085] Table 3 Example 3 Components

[0086]

[0087]

[0088]

[0089] In this embodiment, a method for preparing the above eye cream is also provided, comprising the following steps:

[0090] S1. Add purified water, D-panthenol, niacinamide and chelating agent in phase A to an emulsifying tank, heat to 85° C., stir and heat, then add sodium hyaluronate and the first polyol in phase B, keep warm and stir, and homogenize in vacuum to obtain mixture a for later use;

[0091] S2, add the emulsifier, oil, antioxidant and first preservative in phase C to the pretreatment tank, heat to 85°C, heat and stir to mix evenly, then add the second polyol and Mayinglong Babao powder in phase D, keep warm and stir to obtain mixture b, set aside;

[0092] S3, adding the mixture b obtained in step S2 to the mixture a obtained in step S1, and stirring at 85°C to obtain a mixture c, which is set aside;

[0093] S4, adding the thickener and purified water in phase E to the mixture c obtained in step S3, homogenizing under vacuum, stirring and cooling to 55° C. to obtain a mixture d;

[0094] S5, adding the first aromatic agent and the third polyol in phase F to the mixture d obtained in step S4, homogenizing, stirring and cooling to 45° C. to obtain a mixture e;

[0095] S6, adding the ergothioneine Agrocybe tumefaciens extract, purified water, the second preservative, acetyl hexapeptide-8, cono peptide, oat firming protein in phase G, and the tetrahydromethylpyrimidine carboxylic acid and purified water in phase H to the mixture e obtained in step S5, homogenizing and stirring to obtain a mixture f;

[0096] S7, adding the pH adjuster and purified water in phase I and the third preservative and the second fragrance in phase J to the mixture f obtained in step S6, homogenizing and stirring, and after the treatment is completed, the eye cream is obtained.

[0097] Comparative Example 1

[0098] In this comparative example, except that the Mayinglong Babao raw powder in component D is not added, the other raw materials and steps are the same as those in Example 1.

[0099] Comparative Example 2

[0100] In this comparative example, except that the ergothioneine Agaricus matsutake extract in phase G is not added, the remaining raw materials and steps are the same as those in Example 1.

[0101] Comparative Example 3

[0102] In this comparative example, except that the oat firming protein in phase G is not added, the remaining materials and steps are the same as those in Example 1.

[0103] Test Example 1

[0104] The stability of the eye creams prepared in Examples 1 to 3 was investigated, and the specific test methods and results are as follows:

[0105] Test method: The eye creams prepared in Examples 1 to 3 were put into transparent plastic bottles, and 5 parallel samples were placed under low temperature, low temperature 4°C, cycle 7°C / 45°C / 24h, high temperature 45°C, and room temperature 25°C for 30 days.

[0106] Stability judgment standard: After all samples are restored to room temperature, compared with the 4°C samples, there is no obvious discoloration or taste change after room temperature storage and high and low temperature cycles, no stratification such as water or oil out of the paste, and no particles or precipitates when applied, which is considered stable. The stability test results are shown in Table 4.

[0107] Table 4 Stability test results

[0108] Stability conditions Example 1 Example 2 Example 3 Low temperature -7℃, 30 days normal normal normal Low temperature 4℃, 30 days normal normal normal Normal temperature 25℃, 30 days normal normal normal High temperature 45℃, 30 days normal normal normal Cycle -7℃ / 45℃ / 24h, 30 days normal normal normal

[0109] The test results show that the product prepared in the embodiment of the present invention has no obvious discoloration or taste change under room temperature storage and high and low temperature cycle conditions, and there is no abnormality in the stability inspection. The product has good stability and can be stored for a long time.

[0110] Test Example 2

[0111] The eye creams prepared in Examples 1 to 3 were subjected to an in vitro eye irritation test using the chicken embryo chorioallantoic membrane test.

[0112] 2.1 Test methods

[0113] Use SPF grade Bailaihang chicken fertilized eggs, which should be fresh, clean, intact, and weigh 50g-60g; incubate until 9 days old; check the 9-day-old chicken embryos by egg scanning and mark the position of the air chamber; use tweezers to peel off the eggshell part at the air chamber, add 0.9% NaCl solution to moisten the inner membrane, and then peel it off with tweezers without damaging the blood vessels, otherwise it should be discarded.

[0114] Formal experiment: Select the test method according to the characteristics of the test substance. For transparent liquid, select the reaction time method. This composition is a transparent liquid, and the reaction time method is used. Take 0.3mL of the test substance and drip it directly on the surface of the CAM for 5 minutes. Observe and record the time (s) for bleeding, coagulation, and vascular melting. Repeat for 6 chicken embryos.

[0115] 2.2 Test results

[0116] The irritation results of the eye creams prepared in various embodiments are shown in Table 5.

[0117] Table 5 Irritation results of eye creams prepared in various embodiments

[0118]

[0119]

[0120] The test results show that the chicken embryo irritation score of the eye cream prepared in the embodiments of the present invention is less than 1, and it is non-irritating. The eye cream for regulating circadian rhythm provided by the present invention is non-irritating, safe and reliable.

[0121] Test Example 3

[0122] The content of type I collagen was determined by enzyme-linked immunosorbent assay (ELISA). The detection method refers to standard T / SHRH031-2020 "Test of cosmetic firming and anti-wrinkle efficacy-determination of type I collagen content in fibroblasts in vitro". The specific detection method and results are as follows:

[0123] (1) Sample preparation

[0124] ① Preparation of test samples: Weigh 180 mg of the test samples prepared in Example 1 and Comparative Examples 1 to 3 respectively, dilute to 3 mL with cell culture medium, and then filter and sterilize with a 0.22 μm filter membrane for later use.

[0125] ② Preparation of positive control samples: Take 2 μg of TGFβ1, dissolve it in 40 μL of 10 mM citric acid solution, filter it with a 0.22 μm filter membrane for sterilization, store it at -20°C, avoid repeated freezing, and prepare TGF-β1 stock solution (50 μg / mL); take 4 μL of the stock solution, dissolve it in 996 μL of cell culture medium, and prepare TGF-β1 working solution (200 ng / mL).

[0126] ③ Preparation of solvent control samples: cell culture medium.

[0127] (2) Safe dose screening

[0128] A certain number of HSF cells were seeded in a 96-well plate, usually 1×10 4 cells / well, discard the stock solution after culturing for 24 hours, add 5 different doses (0.375%, 0.75%, 1.5%, 3%, 6%) of the test sample, 200μL per well, and set up a solvent control group. The cell survival rates detected by the test samples of different doses were 94.2%, 94.02%, 93.01%, 90.31%, and 91.50%, respectively.

[0129] According to the cell survival rate in the preliminary experiment, the cell morphology of each dosage group and the actual addition amount, the sample dose (3%) with a cell survival rate ≥ 80% was comprehensively selected as the safe dose.

[0130] (3) Sample testing

[0131] Cell digestion and inoculation: 2×10 4 HSF cells were inoculated at a density of 10 cells / well in a 96-well plate, 100 μL of cell culture medium was added to each well, and the plate was cultured in a CO2 cell culture incubator for 24 h±2 h.

[0132] Drug administration: Discard the culture medium in the 96-well plate and carry out drug administration. Add the culture medium containing the test sample to the test well, the culture medium containing the positive control to the positive control well, and the normal cell culture medium to the solvent control well, 200 μL per well.

[0133] The cell supernatant was collected and ELISA was performed according to the instruction manual of the human type I collagen enzyme-linked immunosorbent assay kit, and the type I collagen upregulation rate calculation formula was used for calculation.

[0134] (4) Test results

[0135] The test results are shown in Table 6.

[0136] Table 6 Summary of Type I collagen content test results

[0137] Group Preparation concentration Type Ⅰ collagen content (μg / L) Increase rate Solvent control group / 5.095±0.032 / Positive control group 0.2 μg / mL 8.013±0.034* 57.27％ Example 1 Test Group 3％ 8.995±0.014* 76.55％ Comparative Example 1 Test Group 3％ 8.231±0.056* 61.55％ Comparative Example 2 Test Group 3％ 7.875±0.033* 54.56％ Comparative Example 3 Test Group 3％ 8.058±0.041* 58.16％

[0138] (Compared with before using the product, *P<0.05)

[0139] As can be seen from the table, the rate of increasing the content of type I collagen in the skin extracellular matrix in the test group of the embodiment of the present invention is significantly better than that in the positive control group and the comparative test group, indicating that the product prepared in the embodiment of the present invention has a good ability to promote the synthesis of type I collagen and has better firming and anti-wrinkle effects.

[0140] Test Example 4

[0141] The cell scratch test is a laboratory technique that studies cell migration, repair ability, and cell-to-cell interactions by scratching a cell monolayer and capturing images through time-lapse microscopy. This test uses mouse fibroblasts (L929 cells) as a cell model to detect the repair efficacy of the test sample through a cell scratch test.

[0142] (1) Sample preparation

[0143] The test sample is a serum-free cell culture medium containing the test substance; the positive control sample is a serum-free cell culture medium containing TGF-β1; the solvent control sample is a serum-free cell culture medium.

[0144] (2) Sample testing

[0145] ① Cell streaking: first thaw the frozen cells, then draw a horizontal line on the back of the 6-well plate, then digest the cells with trypsin protein / EDTA solution, centrifuge, remove the supernatant, add cell culture medium to make a cell suspension, and then inoculate the cell suspension into the 6-well plate, add 3mL of sample to each well, and spread the cells as evenly as possible; when the cells have spread the entire 6-well plate, use a 200μL pipette tip to scratch the center of the horizontal line on the back of the 6-well plate, and then wash the cell debris;

[0146] ② Sample addition: set up positive control, solvent control, Example 1 test group, Comparative Example 1 test group, Comparative Example 2 test group, and Comparative Example 3 test group, all prepared with serum-free culture medium, add 2 mL of sample to each well, put into cell culture incubator for culture, take samples and take pictures at 0, 6, and 24 h;

[0147] ③Result analysis: After opening the image with Image J software, calculate the mean value of the distance between cells or the mean value of the scratch area. The calculation formula for cell migration rate is as follows:

[0148] Cell migration rate (wound healing rate) = (initial scratch area - scratch area at time t) / initial scratch area.

[0149] (3) Test results

[0150] The test results are shown in Table 7.

[0151] Table 7 Summary of cell scratch test results

[0152] Group Cell migration rate (%) Solvent control group 0 Positive control group 22.55 Example 1 Test Group 45.79 Comparative Example 1 Test Group 33.71 Comparative Example 2 Test Group 35.66 Comparative Example 3 Test Group 39.12

[0153] As can be seen from the table, the cell migration rate of the example test group is significantly better than that of the positive control group and the comparative example test group, and the migration rate of L929 cells is as high as 45.79%, indicating that the product prepared in the example has a better repairing effect.

[0154] Test Example 5

[0155] The eye cream prepared in Example 1 and Comparative Examples 1 to 3 was used as a test product to conduct a human efficacy evaluation test.

[0156] 5.1 Evaluation Metrics

[0157] ① Water content of stratum corneum

[0158] Measuring instrument: Corneometer (Courage & Khazaka, Germany). Test 6 times and take the average value. The higher the value, the higher the moisture content of the stratum corneum. Unit: Corneometer Unit, abbreviated as CU.

[0159] ② Transepidermal water loss

[0160] Measuring instrument: Tewameter TM Hex (Courage & Khazaka, Germany). Test three times and take the average value. The lower the value, the less transepidermal water loss. Unit: g / (h·m 2 ).

[0161] ③Wrinkle area

[0162] Measurement instrument: Antera3D HC1023Q skin multifunctional 3D imager (Miravex, Ireland). Image acquisition once (frontal view) and image analysis once.

[0163] The lower the value, the smaller the wrinkles at the corners of the eyes in the test area. The unit is mm 2 .

[0164] ④Wrinkle length

[0165] Measurement instrument: Antera3D HC1023Q skin multifunctional 3D imager (Miravex, Ireland). Image acquisition once (frontal view) and image analysis once.

[0166] The lower the value, the shorter the length of the eye wrinkles in the test area, the unit is mm.

[0167] ⑤Number of wrinkles

[0168] Measurement instrument: Antera3D HC1023Q skin multifunctional 3D imager (Miravex, Ireland). Image acquisition once (frontal view) and image analysis once.

[0169] The lower the value, the fewer wrinkles there are at the corners of the eyes in the test area, and the unit is bars.

[0170] ⑥ Subjective evaluation indicators

[0171] Table 8 Subjective evaluation methods

[0172]

[0173] 5.2 Test methods

[0174] 40 cases with dry, rough and fine lines on the eyes were selected, with an age range of 30-55 years old, wrinkles under the eyes at level 2-5, wrinkles at the corners of the eyes at level 2-4; tear grooves; sensitive skin (Huaxi Questionnaire) were enrolled, and the face had redness, tightness, etc. The test was divided into 4 groups, 10 people in each group; the subjects cleaned their faces and skin around the eyes every morning and evening, waited for the skin around the eyes to dry naturally, took an appropriate amount of eye cream, evenly applied it to the skin around the eyes, and gently massaged the skin around the eyes until absorbed. During the test, it is forbidden to use other types of cosmetics with one of the anti-wrinkle effects on the test area, and other skin care habits do not need to be changed. The test environment is 20.2℃~22.0℃, humidity 40%~60%RH, and the test results are statistically analyzed using SPSS software.

[0175] The specific test methods and time are as follows:

[0176] ①D0 test

[0177] Static wrinkle test: The subject remains still and expressionless, and Antera 3D is used to take 3D pictures of the subject's eye tail and eye for analysis to obtain static wrinkle data.

[0178] Dynamic wrinkle test: The subject keeps smiling with 8 teeth showing, and Antera 3D is used to take 3D pictures of the end of the eye and the eye of the subject for analysis to obtain dynamic wrinkle data.

[0179] Periocular elasticity, moisture content, and transepidermal water loss tests: The skin moisture content and transepidermal water loss rate are tested at designated test sites.

[0180] ②T 10min 、T 120min Test: Antera 3D image acquisition of dynamic lines was performed after using the product for 10 minutes and 120 minutes.

[0181] ③D14, D28 test: Conduct static wrinkle test and periorbital elasticity, water content, and transepidermal water loss test again 14 days and 28 days after using the product.

[0182] 5.3 Test results

[0183] 5.3.1 Test results of moisture content of stratum corneum

[0184] The test results of the moisture content of the stratum corneum are shown in Table 9. As can be seen from the table, compared with before using the product, after using the example product for 28 days, the moisture content of the stratum corneum increased significantly by 43.49%, and the product has a good eye moisturizing effect; while after using the comparative example product for 28 days, the increase in the moisture content of the stratum corneum is not as good as that of the example product, which shows that the moisturizing effect of the example product of the present invention on the eye skin is worse than that of the comparative example product.

[0185] Table 9 Test results of moisture content of stratum corneum before and after using the product (X±S, n=10)

[0186]

[0187] (Compared with before using the product, *P<0.05)

[0188] 5.3.2 Transepidermal water loss test results

[0189] The test results of transepidermal water loss are shown in Table 10. In human experiments, the detection of transepidermal water loss TEWL can reflect the changes in the barrier function of the stratum corneum. It can be seen from the table that compared with before using the product, after using the example product for 28 days, the periocular transepidermal water loss was significantly reduced by 29.94%, and the product had a good skin barrier repair effect and a fast onset; while after using the comparative example product for 28 days, the improvement rate of periocular transepidermal water loss was not as good as that of the example product. It can be seen that the example product of the present invention has a better effect on the periocular skin barrier repair than the comparative example product.

[0190] Table 10 Transepidermal water loss test results before and after using the product (X±S, n=10)

[0191]

[0192] (Compared with before using the product, *P<0.05)

[0193] 5.3.4 Dynamic pattern detection results

[0194] The results of the dynamic wrinkle detection around the eyes are shown in Tables 11 to 13. As can be seen from the table, after using the product of the embodiment of the present invention, the wrinkle area, wrinkle length and wrinkle number around the eyes are significantly reduced within 10 minutes of use, and the product has a good instant anti-aging and wrinkle removal effect. The instant wrinkle removal effect of the product of the embodiment of the present invention on the skin around the eyes is better than that of the comparative example product.

[0195] Table 11 Wrinkle area test results before and after using the product (X±S, n=10)

[0196]

[0197] (Compared with before using the product, *P<0.05)

[0198] Table 12 Wrinkle length test results before and after using the product (X±S, n=10)

[0199]

[0200] (Compared with before using the product, *P<0.05)

[0201] Table 13 Wrinkle quantity test results before and after using the product (X±S, n=10)

[0202]

[0203] (Compared with before using the product, *P<0.05)

[0204] 5.3.5 Static grain detection results

[0205] The results of the static wrinkle test around the eyes are shown in Tables 14 and 15. As can be seen from the table, after using the product of the embodiment of the present invention, the wrinkle area, wrinkle length and wrinkle number around the eyes were significantly reduced at 14 days and 28 days, and the product has a good long-term anti-aging and wrinkle removal effect. The long-term wrinkle removal effect of the product of the embodiment of the present invention on the skin around the eyes is better than that of the comparative example product.

[0206] Table 14 Wrinkle area test results before and after using the product (X±S, n=10)

[0207]

[0208]

[0209] (Compared with before using the product, *P<0.05)

[0210] Table 15 Wrinkle length test results before and after using the product (X±S, n=10)

[0211]

[0212] (Compared with before using the product, *P<0.05)

[0213] Table 16 Wrinkle quantity test results before and after using the product (X±S, n=10)

[0214]

[0215] (Compared with before using the product, *P<0.05)

[0216] 5.3.6 Self-assessment results

[0217] After 28 days of trial use of the test product, the subjects were asked to fill out a questionnaire with options including strongly agree, somewhat agree, neither agree nor disagree, somewhat disagree, and strongly disagree, and the percentage of subjects who strongly agreed and somewhat agreed was calculated. The self-assessment results are shown in Table 17.

[0218] Table 17 Self-evaluation feedback on the skin condition around the eyes after using the product for 28 days

[0219]

[0220]

[0221] It can be seen from Table 17 that after the subjects used the product for 28 days, 100% of the subjects were satisfied with the effect of the example product (including very satisfied and relatively satisfied), and all the subjects in the example group did not experience any adverse reactions or discomfort on the eye skin during use.

[0222] 5.3.7 Subject Image Examples

[0223] Select any subject in the Example 1 group, and use the skin multifunctional 3D imager to collect the dynamic wrinkle detection results. Figure 1 , from left to right in the figure, they are before use, 10 minutes after use, and 120 minutes after use. As can be seen from the figure, the product of the embodiment of the present invention can instantly smooth the wrinkles around the eyes of the test subject, indicating that the composition of the present invention has a good instant wrinkle removal effect.

[0224] The static grain detection results are shown in Figure 2 , from left to right in the figure are before use, after 14 days of use and after 28 days of use. It can be seen from the figure that the product of the embodiment of the present invention can smooth the wrinkles around the eyes of the subjects in a long-term manner, indicating that the composition of the present invention has a good long-term wrinkle-removing effect.

[0225] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of individual raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

Claims

1. An anti-aging and repairing cosmetic composition, characterized in that: In parts by weight, it is prepared from the following raw materials:

2. The anti-aging and repairing cosmetic composition according to claim 1, characterized in that: In parts by weight, it is prepared from the following raw materials:

3. An anti-aging and repairing cosmetic composition according to claim 1 or 2, characterized in that: In parts by weight, the Mayinglong Babao raw powder is obtained by mixing zinc carbonate hydroxide, amber powder and pearl powder in a mass ratio of 80-100:0.1-2:0.1-2.

4. Use of the cosmetic composition according to any one of claims 1 to 3 in the preparation of eye cream.

5. An eye cream, characterized in that: The cosmetic composition is prepared from the cosmetic composition according to any one of claims 1 to 3 and auxiliary materials.

6. The eye cream according to claim 1, characterized in that The auxiliary material is selected from one or more of emulsifiers, polyols, oils, thickeners, preservatives, pH regulators, fragrances, chelating agents, antioxidants, and purified water.

7. The eye cream according to claim 6, characterized in that The emulsifier is selected from one or more of cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 stearate; The polyol is selected from one or more of glycerol, 1,3-propylene glycol, and butylene glycol; The oil is selected from one or more of dimethicone, dimethicone alcohol, cyclopentasiloxane, butylene glycol dicaprylate / dicaprate, hydrogenated polyisobutene, isopropyl myristate, caprylic / capric triglyceride, isononyl isononanoate, C12-15 alcohol benzoate, neopentyl glycol dicaprylate dicaprate, squalane, and avocado butter; The thickener is selected from one or more of polyacrylamide, C13-14 isoparaffin, laureth-7, polyacrylate crosspolymer-6, acrylic acid (ester) / C10-30 alkyl acrylate crosspolymer, xanthan gum, hydroxyethyl cellulose, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, sorbitan isostearate, ammonium acryloyldimethyl taurate / VP copolymer, and sodium polyacryloyldimethyl taurate; The pH regulator is selected from one or more of tartaric acid, citric acid, and malic acid; The aromatic agent is selected from one or two of borneol and essence; The chelating agent is selected from one or more of disodium EDTA and caprylhydroxamic acid; The antioxidant is selected from one or more of butylated hydroxytoluene, butylated hydroxyanisole and tocopherol acetate. The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin, chlorphenesin, methylparaben, p-hydroxyacetophenone, propylparaben, 1,2-hexanediol, and sorbitan octanoate.

8. The eye cream according to claim 7, characterized in that In parts by weight, the amount of each auxiliary material is as follows: 2 to 6 parts of emulsifier, 6 to 15 parts of polyol, 8 to 15 parts of oil, 0.1 to 1 part of thickener, 1 to 3 parts of preservative, 0.01 to 0.5 parts of pH adjuster, 0.1 to 1 part of fragrance, 0.01 to 0.1 part of chelating agent, 1 to 5 parts of antioxidant, and 5 to 95 parts of purified water.

9. A method for preparing the eye cream according to any one of claims 5 to 8, characterized in that: The steps include: S1. Add purified water, D-panthenol, niacinamide and chelating agent in phase A to an emulsifying tank, stir and heat, then add sodium hyaluronate and the first polyol in phase B, keep warm and stir, and homogenize in vacuum to obtain mixture a for later use; S2, add the emulsifier, oil, antioxidant and first preservative in phase C to the pretreatment tank, heat and stir to mix evenly, then add the second polyol and Mayinglong Babao original powder in phase D, keep warm and stir to obtain mixture b, set aside; S3, adding the mixture b obtained in step S2 to the mixture a obtained in step S1, stirring at a temperature of 100 °C to obtain a mixture c, which is set aside; S4, adding the thickener and purified water in phase E to the mixture c obtained in step S3, homogenizing under vacuum, stirring and cooling to obtain a mixture d; S5, adding the first aromatic agent and the third polyol in phase F to the mixture d obtained in step S4, homogenizing, stirring and cooling to obtain a mixture e; S6, adding the ergothioneine Agrocybe tumefaciens extract, purified water, the second preservative, acetyl hexapeptide-8, cono peptide, oat firming protein in phase G, and the tetrahydromethylpyrimidine carboxylic acid and purified water in phase H to the mixture e obtained in step S5, homogenizing and stirring to obtain a mixture f; S7, adding the pH adjuster and purified water in phase I and the third preservative and the second fragrance in phase J to the mixture f obtained in step S6, homogenizing and stirring, and after the treatment is completed, the eye cream is obtained.

10. The preparation method according to claim 9, characterized in that: In step S1, the temperature of heat preservation and stirring is 80-85°C; In step S2, the temperature of heating and stirring is 80-85°C; In step S3, the temperature of heat preservation and stirring is 80-85°C; In step S4, the temperature is lowered to 50-55°C; In step S5, the temperature is lowered to 40-45°C with stirring.

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