Skin care target-based micro-ecological composition with soothing and repairing effects

Abstract

The invention relates to the field of A61K8 / 18, in particular to a skin care target-based micro-ecological composition with soothing and repairing effects, which is at least prepared from the following raw materials in parts by weight: 1 to 40 parts of prebiotics, 0 to 20 parts of probiotics, 0.1 to 3 parts of auxiliaries and 50 to 100 parts of solvent, wherein the prebiotics at least comprise at least one of dextran, ribose, inulin and ectoine, the microecological balance of the skin is effectively regulated, meanwhile, the effects of repairing the skin barrier, relieving redness, stabbing pain and itching, resisting oxidation and the like are achieved, and the use requirements of the sensitive skin are better met.

Description

Technical Field

[0001] The present invention relates to the field of A61K8 / 18, and in particular to a microecological composition with soothing and repairing effects based on skin care targets. Background Art

[0002] In recent years, environmental pollution, life pressure, bad living habits and other reasons have led to a sharp increase in the number of people with sensitive skin. The International Forum for the Study of Itch (IFSA) defines sensitive skin as: an unpleasant feeling (stinging, burning, pain, itching, tingling, etc.) produced by the skin in response to external stimuli. The "Chinese Expert Consensus on the Diagnosis and Treatment of Sensitive Skin" points out that sensitive skin specifically refers to a high-reaction state of the skin under physiological or pathological conditions, mainly occurring on the face. The clinical manifestations are subjective symptoms such as burning, stinging, itching and tightness when the skin is stimulated by physical, chemical, mental and other factors, with or without objective signs such as erythema, scaling, and capillary dilation.

[0003] The nursing targets of sensitive skin are reflected in three aspects: regulating the balance of skin microecology, repairing the skin barrier, and soothing the skin, i.e. reducing redness, stinging and itching. First, balancing the microecology means paying attention to the changes in the skin microbial flora, protecting the diversity of the skin flora, upregulating the expression of antimicrobial peptides and epidermal filaggrin, enhancing the barrier, broad-spectrum antibacterial, and restoring immune function; repairing the barrier means optimizing and supplementing physiological lipids or non-physiological lipids, using transdermal active substances to endogenously regulate the expression of barrier-related proteins, reduce transepidermal water loss, and improve and repair the skin's physical barrier; the soothing mechanism mainly starts from reducing the content of inflammatory factors, inhibiting immune regulation such as inflammatory mediators, and regulating skin nerve function, improving skin redness, stinging and itching, etc. A Chinese patent (publication number CN116270409A) discloses an anti-allergic composition with soothing and repairing effects and its application, including: 0.00001%-6% sugars and 0.00001%-2% fermentation products, achieving a synergistic effect of soothing and repairing the skin barrier, and solving sensitive skin problems. A Chinese patent (authorization announcement number CN117503663B) discloses a skin barrier repair composition, a skin care product containing the composition and a preparation method thereof, which repairs the skin barrier by combining a microcapsule complex, tea polyphenol-modified α-linolenic acid, Centella asiatica extract, chamomile extract, ganoderma lucidum extract, moisturizing ingredients, adjuvants and water. However, the emphasis is mainly on the mechanism targets related to the skin physical barrier and anti-inflammatory, and there is a lack of research and application on the microecological skin care mechanism. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a microecological composition with soothing and repairing effects based on skin care targets, which effectively regulates the balance of skin microecology, while also having the effects of repairing the skin barrier and reducing redness, stinging and itching, and anti-oxidation, so as to better meet the use needs of sensitive skin.

[0005] The present invention provides a microecological composition with soothing and repairing effects based on skin care targets. The raw materials for its preparation include at least 1-40 parts of prebiotics, 0.1-20 parts of probiotics, 0.1-3 parts of adjuvants, and 50-100 parts of solvents, wherein the prebiotics include at least one of glucan, ribose, inulin, and ectoine.

[0006] As a preferred technical solution, the raw materials for preparing the microecological composition include at least 10-30 parts of prebiotics, 2-15 parts of probiotics, 0.5-2 parts of auxiliary agents, and 60-80 parts of solvents, calculated by weight.

[0007] As a preferred technical solution, the mass proportion of prebiotics in the microecological composition is 3-10%, preferably 4-6%.

[0008] As a preferred technical solution, the prebiotic is selected from at least one of milk exopolysaccharides, peptidoglycan, lactic acid, acetic acid, short-chain fatty acids, bacteriocins, lactobacillus / soymilk fermentation filtrate, bifid yeast fermentation lysate, and plant lactobacillus HEAL19.

[0009] As a preferred technical solution, the mass proportion of prebiotics in the microecological composition is 15-30%, preferably 17.5-25%.

[0010] As a preferred technical solution, the prebiotic is a combination of dextran, inulin, and ectoine.

[0011] Preferably, the mass ratio of the dextran, inulin and ectoine is (1-5):(5-15):(5-15), preferably (2-4):(6-12):10.

[0012] Preferably, the glucan is at least one of α-glucan and β-glucan, preferably α-glucan, specifically α-glucan oligosaccharide.

[0013] Preferably, the inulin is extracted from chicory.

[0014] Preferably, the ectoine (ectoine) is obtained by fermenting the probiotic Bacillus clausii which is specially produced for aerospace breeding.

[0015] As a preferred technical solution, the auxiliary agent is selected from at least one of a humectant, a preservative, a thickener, a surfactant, a chelating agent, a pH regulator, and an emollient.

[0016] Preferably, the preservative is a combination of 1,2-hexanediol and p-hydroxyacetophenone. Preferably, the mass ratio of 1,2-hexanediol to p-hydroxyacetophenone is 1:(0.5-2), preferably 1:1.

[0017] As a preferred technical solution, the solvent is at least one of water, glycerol, 1,3-butylene glycol, propylene glycol, pentanediol, and caprylyl glycol, preferably water.

[0018] There are three main approaches to regulating the balance of the microecology: (1) changes in the skin microbial flora - focusing on flora diversity, protecting beneficial bacteria and inhibiting harmful bacteria; (2) expression of skin antimicrobial peptides - broad-spectrum antibacterial, enhancing skin immune function; (3) metabolite production - the use of probiotics to upregulate the expression of barrier-related filaggrin.

[0019] This formula develops soothing products from the perspective of microecology, while also having moisturizing and repairing effects. The present invention optimizes the prebiotics in the system to be a combination of α-glucan oligosaccharides, inulin and tetrahydromethylpyrimidine carboxylic acid (ectoine), and cooperates to achieve the regulation of microecological balance through the above three mechanisms. Specifically, through the cooperation of the three, it can promote the growth of saprophytic bacteria (beneficial bacteria) and fermentation lactobacillus of the skin, competitively inhibit pathogenic bacteria, and activate the expression of antimicrobial peptide β-defensin (2 and 3), further supplement and strengthen the regulation of microecological balance (1) and (2), achieve a broad-spectrum antibacterial effect, and at the same time play a protective effect on bacterial diversity.

[0020] Furthermore, by introducing plant lactobacillus HEAL19 and further controlling its added amount, the provided microecological composition can activate specific interactions, regulate the expression of antimicrobial peptides related to skin health (several antimicrobial peptides from the S100 family protein and RNase A superfamily), and can also act as a prebiotic to upregulate the expression of barrier-associated filaggrin, thereby creating a comfortable environment for probiotics, which is in line with the regulation of microecological balance (2) and (3).

[0021] Furthermore, the microecological composition provided by the present invention balances the microorganisms in the microecological composition and the irritation of the microecological composition by controlling the addition amount of prebiotics and probiotics in the composition, and introducing 1,2-hexanediol and p-hydroxyacetophenone, thereby avoiding the irritation problem caused by the addition of a large amount of preservatives and ensuring the stability of the product.

[0022] In addition, the microecological combination provided by the present invention, through the coordinated action of various raw materials, can strengthen the barrier, reduce transepidermal water loss, improve stratum corneum hydration, increase cortical content, improve skin texture, and activate corresponding immune responses. It has significant effects in anti-inflammation, improving stinging, redness and itching, etc., and meets the mechanism targets of repairing the skin barrier and soothing the skin.

[0023] The preparation method of the microecological composition with soothing and repairing effects based on skin care targets is as follows: prebiotics, probiotics, adjuvants and solvents are stirred and mixed to obtain the composition.

[0024] Beneficial Effects

[0025] 1. The present invention provides a microecological composition with soothing and repairing effects based on skin care targets, which effectively regulates the balance of skin microecology, while also having the effects of repairing skin barrier and reducing redness, stinging and itching, and anti-oxidation, so as to better meet the use needs of sensitive skin.

[0026] 2. This formula develops soothing products from the perspective of microecology, while also having moisturizing and repairing effects. The present invention optimizes the prebiotics in the system to be a combination of α-glucan oligosaccharides, inulin and tetrahydromethylpyrimidine carboxylic acid (ectoine), and cooperates to achieve the regulation of microecological balance through the above three mechanisms. Specifically, through the cooperation of the three, it can promote the growth of saprophytes (beneficial bacteria) on the skin and the growth of fermented lactobacilli, competitively inhibit pathogenic bacteria, and activate the expression of antimicrobial peptide β-defensin (2 and 3), further supplementing and strengthening the regulation of microecological balance (1) and (2), achieving a broad-spectrum antibacterial effect, while exerting the protection effect of bacterial diversity.

[0027] 3. The present invention introduces plant lactobacillus HEAL19 and further controls its addition amount, so that the provided microecological composition can activate specific interactions, regulate the expression of antimicrobial peptides related to skin health (several antimicrobial peptides from S100 family proteins and RNase A superfamily), and can also act as a prebiotic to upregulate the expression of barrier-related filaggrin, thereby creating a comfortable environment for probiotics, which is in line with the regulation of microecological balance (2) and (3).

[0028] 4. The microecological composition provided by the present invention balances the microorganisms in the microecological composition and the irritation of the microecological composition by controlling the addition amount of prebiotics and probiotics in the composition, and introducing 1,2-hexanediol and p-hydroxyacetophenone, thereby avoiding the irritation problem caused by the addition of a large amount of preservatives and ensuring the stability of the product.

[0029] 5. The microecological combination provided by the present invention, through the coordinated action of various raw materials, can strengthen the barrier, reduce transepidermal water loss, improve stratum corneum hydration, increase cortical content, improve skin texture, and activate corresponding immune responses. It has significant effects in anti-inflammation, improving stinging, redness and itching, etc., and meets the mechanism targets of repairing the skin barrier and soothing the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a trend chart of cell viability in the repair test-cell proliferation.

[0031] Figure 2 This is a comparison chart of hyaluronidase inhibition rate in the soothing test-hyaluronidase inhibition test.

[0032] Figure 3 This is a ROS clearance trend graph in the antioxidant test-ROS clearance rate. The concentrations 1-10 in the graph correspond to the concentrations in Table 8. DETAILED DESCRIPTION

[0033] The information of the raw materials used in the examples of the present invention is as follows:

[0034] Inulin and α-glucan oligosaccharide were derived from the ecological nutrient solution of Occelis International Ltd., France;

[0035] Ectohydromethylpyrimidine carboxylic acid was from Tiansheng Biotechnology Co., Ltd.;

[0036] Lactobacillus plantarum HEAL19 was from Symrise Ltd.;

[0037] The remaining raw materials are commercially available.

[0038] Examples 1-3

[0039] Embodiments 1-3 of the present invention provide a microecological composition with soothing and repairing effects based on skin care targets. The raw materials for its preparation are shown in Table 1 in parts by weight.

[0040] Table 1

[0041]

[0042] The preparation method of the microecological composition with soothing and repairing effects based on skin care targets is as follows: prebiotics, probiotics, preservatives and solvents are stirred and mixed to obtain the composition.

[0043] Performance Testing

[0044] 1. Repair test - cell proliferation

[0045] 1.1 Materials

[0046] 1.1.1 Test system

[0047] The cells used in this experiment were human dermal fibroblasts, provided by Boxi Biotechnology Co., Ltd.

[0048] 1.1.2 Main reagents

[0049] DMEM low-glucose medium (Gibco), fetal bovine serum (Gibco), penicillin-streptomycin double antibody (Gibco), trypsin (Gibco), DPBS (Biosharp), MTT (Biosharp), DMSO (Biosharp), and trypan blue (Thermo).

[0050] 1.1.3 Main equipment

[0051] CO2 incubator (Shanghai Boxun, BC-J160), biological safety cabinet (Shanghai Shangdao, BHC-1300ⅡA2), microplate reader (TECAN, Infinite F50), centrifuge (Hunan Xiangyi, H1850R), cell counter (Thermo, CountessⅢ), microscope (Olympus, CKX53), water bath (Jiangyin Poly Scientific Research Instrument Co., Ltd., BHS-4), -80℃ ultra-low temperature refrigerator (Aucma, DW-86L290).

[0052] 1.1.4 Samples

[0053] The sample information is shown in Table 2:

[0054] Table 2 Test sample information

[0055]

[0056] 1.2 Cytotoxicity assay

[0057] 1.2.1 Cell recovery

[0058] The human dermal fibroblasts were revived and inoculated into T75 flasks at 2×10^6 / flask and cultured in a 37°C, 5% CO2 incubator for 3 days.

[0059] 1.2.2 Laying the boards

[0060] Culture until the cell fusion rate is about 90%, digest the cells with 0.25% trypsin, collect them by centrifugation, resuspend them in appropriate amount of culture medium and count them. Take 4.8×10 cells from a 96-well plate. 6 Resuspend the cells in 12 mL of basal medium, and each 100 μL / well contains 4 × 10 4 Each test concentration was plated with 3 replicate wells and cultured in a 37°C, 5% CO2 incubator for 24 h.

[0061] 1.2.3 Experimental Grouping

[0062] The experiment set up negative control, positive control, test sample and blank control.

[0063] 1.2.4 Liquid preparation

[0064] Complete culture medium was used to prepare samples of different concentrations according to the test concentration setting, and the samples were sterilized by filtration, as shown in Table 3.

[0065] Table 3 Test concentration table

[0066]

[0067] 1.2.5 Drug administration

[0068] When the cell fusion rate in the 96-well plate reaches 40%-60%, the drug is administered, the original culture medium is discarded, 100 μL of culture medium is added to each well of the negative control; 100 μL of culture medium containing 10wt% dimethyl sulfoxide (DMSO) is added to each well of the positive control; 100 μL of culture medium containing samples of corresponding concentrations is added to each well of the sample group; the blank control has no cell inoculation and only 100 μL of culture medium is added. After the drug administration is completed, the 96-well plate is placed in a 37°C, 5% (V / V) CO2 incubator for 20 hours.

[0069] 1.2.6 Detection

[0070] Prepare 20mL MTT solution in advance: Dissolve 0.1g MTT powder in 20ml PBS and filter to sterilize. The final concentration is 5mg / mL and store in the dark for later use. After 20h of cell culture, add 20μL / well MTT solution to the 96-well plate and continue to culture for 4h in a 37℃, 5% (V / V) CO2 incubator. After 4h, remove the cells, carefully discard the supernatant, add 150μL / well DMSO and blow until fully mixed, and place at 25℃ in the dark for 30min. Use a pipette to mix the cell suspension again, and measure the absorbance of each well at OD492nm in an enzyme-linked immunosorbent assay.

[0071] 1.2.7 Calculation of relative cell viability

[0072] Calculate according to the formula: relative cell survival rate % = (sample OD-blank control OD) / (negative control OD-blank control OD)×100%

[0073] 1.3 Toxicity results

[0074] Eight dosing concentrations were set and cytotoxicity tests were carried out on human dermal fibroblasts. The MTT test results are shown in Table 4. The cell viability trend is shown in Figure 1 shown.

[0075] Table 4 MTT test results

[0076]

[0077]

[0078] Note: t-test was used for statistical analysis. When the Sample group was compared with the NC group, P value < 0.05 indicated a significant difference, which was indicated by "*", and P value < 0.01 indicated an extremely significant difference, which was indicated by "**".

[0079] 1.4 Conclusion

[0080] The cell viability of some samples at the tested concentrations was greater than 100%. Compared with the NC group, the P value was less than 0.05, showing a significant difference, indicating that the composition in the above examples has a certain ability to promote cell proliferation at a certain concentration, and it can be considered that it has a certain repair effect. Compared with single ectoine, the cell viability of the composition of the present invention is higher and the repair ability is stronger.

[0081] 2. Soothing Test-Hyaluronidase Inhibition Test

[0082] 2.1. Test Principle

[0083] According to the immune response mechanism, allergic reactions are divided into type I, type II, type III and type IV. Most allergic reactions are type I allergic reactions related to the activity of hyaluronidase in the body. The degradation products of hyaluronic acid can increase the inflammatory response, and the degree of the reaction depends on the activity of hyaluronidase. Therefore, the ability of a substance to inhibit the activity of hyaluronidase can be used as an indicator of its anti-inflammatory response. In an acidic environment, hyaluronidase can hydrolyze the substrate and convert it into N-acetylglucosamine, which produces a color reaction with dimethylaminobenzaldehyde. The experiment evaluates the ability of the sample to inhibit the activity of hyaluronidase by comparing the changes in absorbance before and after the reaction, thereby judging its soothing effect.

[0084] 2.2 Materials

[0085] 2.2.1 Test system

[0086] Positive control: 2 mg / mL dipotassium glycyrrhizinate aqueous solution

[0087] 2.2.2 Main reagents

[0088] Glacial acetic acid; anhydrous sodium acetate; hyaluronidase (biotechnology grade, 300U / mg-500U / mg); sodium hyaluronate (from Streptococcus equi); sodium carbonate; acetylacetone; p-dimethylaminobenzaldehyde (CAS No. 100-10-7); concentrated hydrochloric acid (37wt%); anhydrous ethanol; anhydrous calcium chloride; sodium hydroxide; dipotassium glycyrrhizinate (purity ≥98wt%).

[0089] 2.2.3 Main instruments

[0090] Microplate reader, analytical balance, adjustable pipette, 96-well plate, test tubes, water bath.

[0091] 2.2.4 Samples

[0092] The sample information is shown in Table 5.

[0093] Table 5 Information of samples to be tested

[0094]

[0095] 2.3 Experimental procedures

[0096] 2.3.1 Solution preparation

[0097] The sample concentration in this experiment was 10% (V / V).

[0098] 2.3.2 Sample addition

[0099] Trial Groups

[0100] The experiment set up sample group, sample background group, enzyme reaction group and solvent background group. Three replicate wells were required for each sample group of each tested concentration, and three replicate wells were also required for the enzyme reaction group.

[0101] Test steps

[0102] Referring to Table 6, add reagents into the test tube in order and measure the absorbance near 530nm after the reaction is completed.

[0103] Table 6 Sample addition requirements

[0104]

[0105] Calculation of hyaluronidase inhibition rate

[0106]

[0107] 2.4 Experimental Results

[0108] According to the specific experimental method, the hyaluronidase inhibition rate was calculated. The results are shown in Table 7. Figure 2 As shown, the details are as follows:

[0109] Table 7 Summary of hyaluronidase inhibition rate

[0110] Experimental Grouping Mean SD P NC 2.29％ 1.40％ / PC 15.49％ 2.30％ 0.001** Sample A, 10% (V / V) 25.88％ 2.91％ 0.000** Sample B, 10% (V / V) 33.10％ 9.03％ 0.025* Sample C, 10% (V / V) 35.21％ 9.30％ 0.024*

[0111] Note: t-test was used for statistical analysis. When the PC group, Sample group and NC group were compared, P value < 0.05 indicated a significant difference, which was indicated by "*", and P value < 0.01 indicated an extremely significant difference, which was indicated by "**".

[0112] Statistical analysis showed that compared with the NC group, the P value of the PC group was P = 0.001, which was less than 0.05, and the inhibition rate of the PC group was 17.11%, which was within the standard range, verifying the effectiveness of the experimental system; compared with the NC group, the P value of the sample "soothing raw material" was 0.000, which was less than 0.01, showing a very significant difference.

[0113] 2.5 Conclusion

[0114] According to the test results, the hyaluronidase inhibition rate of the PC group (2mg / ml dipotassium glycyrrhizinate) was 15.49%, which was in line with the standard range, verifying the effectiveness of the experimental system; at the test concentration, the average hyaluronidase inhibition rates of the three samples were 25.88%, 33.10% and 35.21%, SD <15%, and compared with the NC group, the P value was <0.05, which means that the samples have a certain soothing effect. Compared with the single tetrahydromethylpyrimidine carboxylic acid, the composition of the present invention has a higher average hyaluronidase inhibition rate and a stronger soothing effect.

[0115] 3. Antioxidant test-ROS clearance rate

[0116] 3.1 Test Principle

[0117] The acceleration of aging is related to reactive oxygen species (ROS), which are a class of active substances containing oxygen atoms, examples of which include peroxides, superoxides, hydroxyl radicals, singlet oxygen, and α-oxygen. Free ROS will attack cell membrane fatty acids, collagen and other structures, accelerating skin aging; therefore, the ability of a sample to scavenge ROS can be used to characterize its firming and anti-wrinkle effects. Salicylic acid can capture the ·OH produced by the typical Fenton reaction to generate colored substances. When antioxidants are present, the generation of colored substances is reduced and the absorbance is reduced. The experiment evaluates the ROS scavenging ability of the sample by comparing the changes in absorbance.

[0118] 3.2 Materials

[0119] 3.2.1 Main reagents

[0120] Ferrous sulfate heptahydrate, hydrogen peroxide, salicylic acid.

[0121] 3.2.2 Main instruments

[0122] Microplate reader (TECAN, Infinite F50), analytical balance, adjustable pipette, 96-well plate.

[0123] 3.3 Experimental procedures

[0124] 3.3.1 Solution preparation

[0125] (1) Sample concentration setting

[0126] Table 8 Sample concentration settings

[0127]

[0128] (2) Positive control

[0129] In this experiment, vitamin C was used as a positive control, and the highest concentration used was 0.5 mg / mL.

[0130] 3.3.2 Sample addition

[0131] Add different solutions to different wells in order as required in the table below, mix well, then add salicylic acid to capture OH, incubate at 37°C for 1 hour, and measure the absorbance at 492 nm.

[0132] Table 9 Sample addition requirements

[0133]

[0134] 3.3.3 Calculation of ROS clearance rate

[0135] ROS clearance rate (%) = 1-[(A1-A2) / A3]

[0136] 3.4 Experimental Results

[0137] According to the specific experimental method, the ROS free radical scavenging rate was calculated. The results are shown in Table 10. The changing trend is shown in Figure 3 As shown, the details are as follows:

[0138] Table 10 Summary of ROS clearance rates

[0139]

[0140]

[0141] 3.5 Conclusion

[0142] According to the test results, the samples of Examples 1-3 have ROS scavenging rates of 32.40%, 37.00% and 32.55% at the highest test concentrations, respectively, and the data parallelism is effective, indicating that they have a certain ability to scavenging ROS, and it can be considered that they have a certain antioxidant effect. Compared with single ectoine, the composition of the present invention has a higher ROS scavenging rate and a stronger antioxidant effect.

[0143] 4. Stability test

[0144] The microecological compositions provided in Examples 1, 2, and 3 were subjected to product stability tests under different tests and conditions. The test conditions and test results are shown in Table 11.

[0145] Table 11

[0146]

[0147]

[0148]

[0149]

[0150]

[0151]

Claims

1. A microecological composition with soothing and repairing effects based on skin care targets, characterized in that: The raw materials for its preparation include at least 1-40 parts of prebiotics, 0.1-20 parts of probiotics, 0.1-3 parts of adjuvants, and 50-100 parts of solvents by weight, wherein the prebiotics include at least one of glucan, ribose, inulin, and ectoine.

2. The microecological composition with soothing and repairing effects based on skin care targets according to claim 1, characterized in that: By weight, the raw materials for preparing the microecological composition include at least 10-30 parts of prebiotics, 2-15 parts of probiotics, 0.5-2 parts of auxiliary agents, and 60-80 parts of solvents.

3. The microecological composition with soothing and repairing effects based on skin care targets according to claim 1 or 2, characterized in that: The mass proportion of prebiotics in the microecological composition is 3-10%.

4. The microecological composition with soothing and repairing effects based on skin care targets according to claim 3, characterized in that: The prebiotics are selected from at least one of milk exopolysaccharide, peptidoglycan, lactic acid, acetic acid, short-chain fatty acids, bacteriocins, lactobacillus / soymilk fermentation filtrate, bifid yeast fermentation lysate, and plant lactobacillus HEAL19.

5. The microecological composition with soothing and repairing effects based on skin care targets according to claim 3, characterized in that: The mass proportion of prebiotics in the microecological composition is 15-30%.

6. The microecological composition with soothing and repairing effects based on skin care targets according to claim 5, characterized in that: The prebiotic is a combination of dextran, inulin and ectoine, and the mass ratio of the dextran, inulin and ectoine is (1-5): (5-15): (5-15).

7. The microecological composition with soothing and repairing effects based on skin care targets according to claim 3, characterized in that: The auxiliary agent is selected from at least one of a moisturizer, a preservative, a thickener, a surfactant, a chelating agent, a pH regulator, and an emollient.

8. The microecological composition with soothing and repairing effects based on skin care targets according to claim 7, characterized in that: The preservative is a combination of 1,2-hexanediol and p-hydroxyacetophenone.

9. The microecological composition with soothing and repairing effects based on skin care targets according to claim 3, characterized in that: The solvent is at least one of water, glycerol, 1,3-butylene glycol, propylene glycol, pentylene glycol and caprylyl glycol.

10. The microecological composition with soothing and repairing effects based on skin care targets according to any one of claims 1 to 3, characterized in that: The preparation method of the microecological composition with soothing and repairing effects based on skin care targets is as follows: prebiotics, probiotics, adjuvants and solvents are stirred and mixed to obtain the composition.

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