Oriental cherry flower extracting solution composition with anti-oxidation effect and preparation method of oriental cherry flower extracting solution composition

Abstract

The invention discloses a plant extract composition, and particularly relates to an oriental cherry flower extract composition and a preparation method thereof, and the oriental cherry flower extract composition is prepared from the following components in parts by weight: 7.22 parts of deionized water, 70.00 parts of oriental cherry flower extract, 6.00 parts of glycerol, 5.00 parts of propylene glycol, 0.08 part of sodium hyaluronate and 0.04 part of carbomer. The feed additive is prepared from the following components in parts by weight: 3.00 parts of a lactobacillus fermentation lysate mixture, 0.02 part of disodium ethylene diamine tetraacetate, 2.50 parts of trehalose, 0.04 part of arginine, 5.00 parts of 1, 3-butanediol, 0.50 part of acetophenone and 0.60 part of a 1, 2-hexanediol solution. The technical problem of providing the oriental cherry extracting solution composition with the effects of resisting oxidation, aging, tumor and the like is solved, and the oriental cherry extracting solution composition provided by the invention has the conventional effects of resisting bacteria and corrosion, resisting static electricity, thickening, moisturizing and locking water, brightening and whitening the skin and the like; meanwhile, the applicant proves that the traditional Chinese medicine composition also has the effects of resisting oxidation, aging, tumors and the like through tests.

Description

Technical Field

[0001] The present invention relates to an extract composition, in particular to a Guanshan cherry extract composition. Meanwhile, the present invention also provides a preparation method of the Guanshan cherry extract composition. Background Art

[0002] Studies have shown that cherry blossom extract has a good inhibitory effect on free radicals on the human body surface, and can also inhibit the formation of melanin, thus delaying skin aging and whitening the skin.

[0003] However, there are no mature skin care products on the market. Different varieties of cherry blossoms have different ingredients and proportions. It is difficult to accurately determine the formula ratio of skin care products using a variety of cherry blossoms. Guanshan cherry blossoms are the only variety of cherry blossoms included in the national new resource food catalog, which can ensure the quality, health and clear variety.

[0004] Therefore, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to solve the above-mentioned deficiencies in the prior art and to provide a Guanshan cherry blossom extract composition and a preparation method thereof.

[0006] In order to achieve the above objectives: In one aspect, the present invention provides a Guanshan cherry blossom extract composition, which is composed of the following components in parts by weight: 7.22 parts of deionized water, 70.00 parts of Guanshan cherry extract, 6.00 parts of glycerin, 5.00 parts of propylene glycol, 0.08 parts of sodium hyaluronate, 0.04 parts of carbomer, 3.00 parts of lactobacillus fermentation lysate mixture, 0.02 parts of disodium ethylenediaminetetraacetic acid, 2.50 parts of trehalose, 0.04 parts of arginine, 5.00 parts of 1,3-butylene glycol, 0.50 parts of cinnamone, and 0.60 parts of 1,2-hexanediol solution; The lactobacillus fermentation lysate mixture is composed of the following components in weight ratio: 35.00 parts of lactobacillus fermentation lysate, 33.50 parts of Sophora japonica fruit extract, 30.00 parts of hydrolyzed millet, 1.00 parts of 1,2-hexanediol, and 0.50 parts of 1,2-pentanediol; The 1,2-hexanediol solution is composed of the following components in weight ratio: 99.50 parts of 1,2-hexanediol and 0.50 parts of water.

[0007] On the other hand, the present invention also provides a method for preparing the above-mentioned Sakura guanshanensis extract composition, which comprises the following steps: (1) Using low-temperature drying equipment to obtain Guanshan cherry blossom extract; (2) adding lactobacillus fermentation lysate, Sophora japonica fruit extract, hydrolyzed millet, 1,2-hexanediol, and 1,2-pentanediol at one time according to the formula ratio to prepare a lactobacillus fermentation lysate mixture; (3) adding 1,2-hexanediol and water in proportion to prepare a 1,2-hexanediol solution; (4) Weigh the deionized water in the formula, heat it to 75-80° C., add glycerol, propylene glycol, sodium hyaluronate, carbomer, disodium edetate, trehalose, arginine, 1,3-butylene glycol, cinnamaldehyde, and 1,2-hexanediol solution in sequence under stirring, and homogenize until completely dissolved; (5) When the temperature drops to 40-45° C., add the Sakura extract, the mixture of the fermentation lysate of lactobacillus, and the 1,2-hexanediol solution, stir, and homogenize to adjust the pH.

[0008] Compared with the prior art, the Guanshan cherry extract composition obtained by the present invention has the following technical effects: The extract of Guanshan cherry blossom contains tender red pigments such as isosapolamine for skin care, cherry blossom polysaccharides for anti-oxidation and anti-aging, and a variety of aromatic compounds.

[0009] Sodium hyaluronate has unique moisturizing properties and can absorb a large amount of water from the dermis. At the same time, it can prevent water evaporation and loss, thereby maintaining a constant water environment in the skin. Sodium hyaluronate can also improve the physiological conditions of the skin, provide a superior external environment for the synthesis of dermal collagen and elastic fibers, and strengthen the supply of nutrients to achieve the effect of skin care and beauty.

[0010] The fermentation lysate of lactobacillus is easily dispersed in water and contains the bacteria and all the active ingredients synthesized during the fermentation process, including peptidoglycan, teichoic acid, protein, phospholipids, sterols, fatty acids, various enzymes, peptides and amino acids, nucleotides, extracellular polysaccharides, etc., which provide rich nutrition for the skin and have anti-aging effects; reduce the number of wrinkles and reduce the depth of wrinkles; improve skin elasticity; brighten the skin tone, rejuvenate and balance the skin tone.

[0011] Sophora fruit extract, namely sophoracarpine, has strong anti-tumor activity.

[0012] Hydrolyzed millet has the effect of reducing wrinkles, spots and pigmentation.

[0013] Both 1,2-hexanediol and 1,3-butylene glycol have moisturizing properties.

[0014] 1,2-Pentanediol is used as a solvent and preservative, has water-locking and antibacterial functions, and can improve water resistance.

[0015] The main function of disodium EDTA is a chelating agent, which can synergize the antiseptic effect. It also has a certain synergistic effect on the antiseptic system and the antioxidant system.

[0016] Trehalose has moisturizing properties and anti-radiation effects. It can protect the skin and promote cytokine repair.

[0017] Arginine has moisturizing, skin softening, anti-wrinkle, whitening and refreshing effects.

[0018] The Guanshan cherry extract composition provided by the present invention has conventional effects such as antibacterial and antiseptic, antistatic, thickening, moisturizing and water locking, skin brightening and whitening. At the same time, the applicant has proved through experiments that the present invention also has anti-oxidation, anti-aging, and tumor effects. DETAILED DESCRIPTION

[0019] The present invention is further described below by means of specific examples. However, it should be understood that these examples are only used for more detailed and specific description and should not be understood as limiting the present invention in any form.

[0020] This section generally describes the materials and test methods used in the experiments of the present invention. Although many materials and operating methods used to achieve the purpose of the present invention are well known in the art, the present invention is still described as detailed as possible. It is clear to those skilled in the art that in the context, if not specifically stated, the materials and operating methods used in the present invention are well known in the art.

[0021] The reagents and instruments used in the following examples are as follows: Reagents: Deionized water was purchased from Shanghai Jingchun Water Treatment Technology Co., Ltd.; Guanshan cherry extract was obtained from Ningbo Haishu District Forestry Specialty Products Society; Glycerol (DG, DAG) was purchased from China Hongtai Chemical Group Co., Ltd.; Propylene glycol (PG) was purchased from Jiangsu Dena Chemical Co., Ltd.; Sodium hyaluronate (HA) was purchased from Bloomage Biotech Co., Ltd.; Carbomer (Carbomer 940) was purchased from Anhui Newman Fine Chemical Co., Ltd.; Ethylenediaminetetraacetic acid disodium (EDTA-2NA) was purchased from Xiangshui Tianyi Biotechnology Co., Ltd.; Trehalose was purchased from Ningbo Loncin Biotechnology Co., Ltd.; Arginine was purchased from Wuxi Jiuxin Biotechnology Co., Ltd.; 1,3-Butanediol, purchased from Jiangsu Dena Chemical Co., Ltd.; Xinxianketone was purchased from Wuhan Penglei Biotechnology Co., Ltd.; Lactobacillus fermentation lysate was purchased from Hangzhou Hesheng Taiyi Biotechnology Co., Ltd.; Sophora japonica fruit extract was purchased from Wuhan Profo Biotechnology Co., Ltd.; Hydrolyzed millet was purchased from Shanghai Yuyu New Materials Technology Co., Ltd.; 1,2-Hexanediol was purchased from Jiangsu Dena Chemical Co., Ltd.; 1,2-Pentanediol, purchased from Jiangsu Dena Chemical Co., Ltd.; instrument: Low temperature drying equipment, Changzhou Yige Drying Equipment Co., Ltd.; Homogenizer, purchased from IKA; pH meter, Bell Analytical Instruments (Dalian) Co., Ltd.; The multifunctional microplate reader, SpectraMax Mini, was purchased from Meigu Molecular Instruments (Shanghai) Co., Ltd.

[0022] The present embodiment provides a Guanshan cherry extract composition, which is composed of the following components in parts by weight: 7.22 parts of deionized water, 70.00 parts of Guanshan cherry extract, 6.00 parts of glycerin, 5.00 parts of propylene glycol, 0.08 parts of sodium hyaluronate, 0.04 parts of carbomer, 3.00 parts of lactobacillus fermentation lysate mixture, 0.02 parts of disodium ethylenediaminetetraacetic acid, 2.50 parts of trehalose, 0.04 parts of arginine, 5.00 parts of 1,3-butylene glycol, 0.50 parts of cinnamone, and 0.60 parts of 1,2-hexanediol solution; The lactobacillus fermentation lysate mixture is composed of the following components in weight ratio: 35.00 parts of lactobacillus fermentation lysate, 33.50 parts of Sophora japonica fruit extract, 30.00 parts of hydrolyzed millet, 1.00 parts of 1,2-hexanediol, and 0.50 parts of 1,2-pentanediol; The 1,2-hexanediol solution is composed of the following components in weight ratio: 99.50 parts of 1,2-hexanediol and 0.50 parts of water.

[0023] The method for preparing the above-mentioned Guanshan cherry extract composition comprises the following steps: (1) Using low-temperature drying equipment to obtain Guanshan cherry blossom extract; (2) adding lactobacillus fermentation lysate, Sophora japonica fruit extract, hydrolyzed millet, 1,2-hexanediol, and 1,2-pentanediol at one time according to the formula ratio to prepare a lactobacillus fermentation lysate mixture; (3) adding 1,2-hexanediol and water in proportion to prepare a 1,2-hexanediol solution; (4) Weigh the deionized water in the formula, heat it to 75-80° C., add glycerol, propylene glycol, sodium hyaluronate, carbomer, disodium edetate, trehalose, arginine, 1,3-butylene glycol, cinnamaldehyde, and 1,2-hexanediol solution in sequence under stirring, and homogenize until completely dissolved; (5) When the temperature drops to 40-45° C., add the Sakura extract, the mixture of the fermentation lysate of lactobacillus, and the 1,2-hexanediol solution, stir, and homogenize to adjust the pH.

[0024] Antioxidant performance test: The present invention uses DPPH free radical and ABTS free radical scavenging rate tests to evaluate the antioxidant properties of the Guanshan cherry extract composition. Both methods are relatively classic antioxidant capacity analysis methods, and are simple to operate, high in precision and repeatability, and reliable in results.

[0025] First, prepare a 0.1mmol / L DPPH ethanol solution, then take 2.0mL of the sample solution (Sakura extract composition) and mix it evenly with 2.0mL of the DPPH ethanol solution. After standing at room temperature in the dark for 20 minutes, use a multifunctional microplate reader to detect the absorbance value A of the mixture at 518nm and record the value. In this test, Trolox was used as the positive control group, and the clearance rate = [1-(A1-A2) / A0] × 100%.

[0026] Secondly, accurately weigh 0.0384g ABTS and 0.0134g potassium persulfate and dilute to 10mL, mix the two reagents in equal amounts, react in the dark for 10h to obtain ABTS+·working solution, and dilute 15 times with PBS before use. Then take 1.0mL sample solution and 3.0mL ABTS+·working solution and mix them evenly, place them in a 25℃ water bath in the dark for 20min, and use a multifunctional microplate reader to detect the absorbance value A of the mixed solution at 734nm, and record the value. Trolox is used as the positive control in this test, and the clearance rate = [1-(A1-A2) / A0]×100%.

[0027] The specific test results are shown in Table 1 below. From the table, it can be seen that the Guanshan cherry extract composition has a significant ability to scavenge DPPH free radicals and ABTS free radicals, and the scavenging rate of oxidative free radicals exceeds 80%, that is, the composition has the ability to directly scavenge reactive oxygen species (ROS) in vitro.

[0028] Table 1 Sample name Hydroxyl radical scavenging rate (%) ABTS free radical scavenging rate (%) Guanshan cherry extract composition 86.8 83.5 Volunteer comments: Thirty female volunteers aged 25 to 50 were selected. Under the same external environmental conditions, an appropriate area on the inner side of the forearm flexion was used as the test area.

[0029] Table 2 below shows the evaluation results of volunteers on the somatosensory effects of the Guanshan cherry blossom extract composition.

[0030] Table 2 Somatosensory effect Number of people Number of people good 26 87% generally 4 13% Difference 0 0 The evaluation results show that the Guanshan cherry blossom extract composition has excellent antioxidant, aging, and tumor effects, can help protect the skin's natural barrier, and has no obvious irritation or damage to the skin, and has good skin adaptability.

[0031] The present invention is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present invention. However, no matter what changes are made in the shape or structure, all technical solutions that are the same or similar to those of the present application fall within the protection scope of the present invention.

Claims

1. A Guanshan cherry extract composition, characterized in that It is composed of the following components in parts by weight: 7.22 parts of deionized water, 70.00 parts of Guanshan cherry extract, 6.00 parts of glycerin, 5.00 parts of propylene glycol, 0.08 parts of sodium hyaluronate, 0.04 parts of carbomer, 3.00 parts of lactobacillus fermentation lysate mixture, 0.02 parts of disodium ethylenediaminetetraacetic acid, 2.50 parts of trehalose, 0.04 parts of arginine, 5.00 parts of 1,3-butylene glycol, 0.50 parts of cinnamone, and 0.60 parts of 1,2-hexanediol solution; The lactobacillus fermentation lysate mixture is composed of the following components in weight ratio: 35.00 parts of lactobacillus fermentation lysate, 33.50 parts of Sophora japonica fruit extract, 30.00 parts of hydrolyzed millet, 1.00 parts of 1,2-hexanediol, and 0.50 parts of 1,2-pentanediol; The 1,2-hexanediol solution is composed of the following components in weight ratio: 99.50 parts of 1,2-hexanediol and 0.50 parts of water.

2. A method for preparing the Guanshan cherry extract composition as claimed in claim 1, characterized in that The steps include: (1) Using low-temperature drying equipment to obtain Guanshan cherry blossom extract; (2) adding lactobacillus fermentation lysate, Sophora japonica fruit extract, hydrolyzed millet, 1,2-hexanediol, and 1,2-pentanediol at one time according to the formula ratio to prepare a lactobacillus fermentation lysate mixture; (3) adding 1,2-hexanediol and water in proportion to prepare a 1,2-hexanediol solution; (4) Weigh the deionized water in the formula, heat it to 75-80° C., add glycerol, propylene glycol, sodium hyaluronate, carbomer, disodium edetate, trehalose, arginine, 1,3-butylene glycol, cinnamaldehyde, and 1,2-hexanediol solution in sequence under stirring, and homogenize until completely dissolved; (5) When the temperature drops to 40-45° C., add the Sakura extract, the Lactobacillus fermentation lysate mixture, and the 1,2-hexanediol solution, stir, and homogenize to adjust the pH.