Gynecological gel for tightening and repairing and preparation method thereof

Abstract

The invention provides a tightening and repairing gynecological gel. Preparation raw materials comprise carbomer, sodium hyaluronate, an animal umbilical cord extract, madecassoside, carnosine, a polygonum cuspidatum root extract, a scutellaria baicalensis root extract, a tea leaf extract, a chamomile flower extract, elastic collagen, arginine, pentanediol, butanediol, glycerin, p-hydroxyacetophenone, capryloyl hydroximic acid and purified water. The gynecological gel can maintain balanced strains, so that the microenvironment of the vagina is kept normal; vaginal atrophy is improved, the tight state of the vagina is recovered, and vaginal dryness is relieved; damaged mucosa can be repaired, the natural defense function of women is enhanced, and gynecological infection is prevented. The gynecological gel has good biocompatibility, cell affinity and mechanical properties, and can supplement collagen to female private parts, repair damaged vaginal mucosa and promote wound healing; the vagina can be moisturized and moistened, the looseness and dryness of the vagina are improved, the micro-ecological balance of the vagina is adjusted, and the immunity of a female reproductive system is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of medical gels and relates to a firming and repairing gynecological gel and a preparation method thereof. Background Art

[0003] For a long time, my country's traditional gynecological topical drugs have been dominated by Chinese patent medicines, antibiotics and chemical synthetic drugs. Among them, chemical synthetic drugs dominate the domestic gynecological topical drugs market. However, these three types of drugs have certain disadvantages to a greater or lesser extent. Chinese patent medicines have been dominant in the past few years due to their mild efficacy, small side effects, and compliance with the long-standing medication habits of Chinese women. However, Chinese medicine preparations generally have the problem of slow efficacy and relatively long treatment period. Excessive use of antibiotics is prone to drug resistance. Once drug-resistant strains are produced, the condition cannot be effectively controlled even if the drug dosage is continuously increased. In addition, antibiotics are easy to destroy the balance of vaginal flora, and the recurrence rate of inflammation after use is high. Although chemical synthetic drugs are quick to take effect, they have relatively large toxic side effects and cannot identify "enemies and friends". While killing pathogens, they also kill beneficial bacteria, destroying the balance of vaginal flora, resulting in a high recurrence rate of inflammation and difficulty in curing after recurrence. In addition, chemical synthetic drugs may cause heavy metal residues, and long-term use will affect women's physical and mental health.

[0004] In recent years, the application research of gel has attracted the attention of scholars at home and abroad. The texture of gel is conducive to adhesion, and it dissolves and absorbs more quickly; at the same time, gel can stick out dirt, effectively remove internal dirt, deeply purify and clean, and achieve the purpose of moisturizing and smoothing. In addition, gel can also restore the healthy state of flexibility and firmness through sustained release absorption, restore elasticity and firm contours, restore self-repair, and treat gynecological diseases. However, most of the existing gynecological gels contain antibiotics, resulting in poor treatment effects, easy to produce drug resistance, and difficult to cure. Summary of the invention

[0005] The purpose of the present invention is to provide a firming and repairing gynecological gel and a preparation method thereof, so as to solve the problem that the existing gynecological gel has poor therapeutic effect.

[0006] To achieve the above object, the present invention adopts the following technical solutions: The invention provides a firming and repairing gynecological gel. The raw materials for preparing the gynecological gel include: carbomer, sodium hyaluronate, animal umbilical cord extract, madecassoside, carnosine, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea extract, anthemis nobilis extract, elastic collagen, arginine, pentylene glycol, butylene glycol, glycerol, p-hydroxyacetophenone, caprylhydroxamic acid and purified water.

[0007] Among them, the functions of each preparation raw material in the gynecological gel are as follows: Carbomer: It is a high molecular polymer of acrylic acid bonded to allyl sucrose or pentaerythritol allyl ether. It is a loose, white, slightly acidic powder with moisturizing and molding effects. It is used as a gel matrix in this application.

[0008] Sodium hyaluronate: It is a kind of glucuronic acid, which is widely distributed in tissues and organs such as the placenta, amniotic fluid, lens, cartilage tissue, and dermis of the skin. Sodium hyaluronate has moisturizing and lubricating properties, which can make the skin smooth and lubricated, protect the skin, and increase the self-cleaning and lubricating effect of the vagina. At the same time, sodium hyaluronate can also promote the proliferation and differentiation of epidermal cells, remove oxygen free radicals, and restore local epithelial cells.

[0009] Animal umbilical cord extract: This extract is taken from bovine umbilical cord tissue and is an animal-derived stem cell exosome. Animal umbilical cord extract carries a variety of genetic materials and has important biological functions, such as anti-inflammatory, repair and regeneration. Animal umbilical cord extract can also maintain cell stability, transmit rejuvenation cell signals to skin cells, delay skin aging, and achieve the effect of repairing and firming.

[0010] Madecassoside: A triterpenoid saponin component contained in Centella asiatica, it has a wide range of anti-inflammatory, anti-ulcer and antioxidant activities. It can repair skin damage and scars, promote fibroblast regeneration, accelerate wound healing, and can effectively repair skin ulcers caused by inflammation such as vaginitis and cervicitis, and accelerate wound healing.

[0011] Carnosine: It is a dipeptide composed of β-alanine and L-histidine, with the advantages of small molecular weight, stable properties, and easy absorption. Carnosine can regulate the pH value in the body, chelate metal ions, inhibit non-enzymatic glycosylation, and has physiological activities such as antioxidant properties. Carnosine can prevent or alleviate skin aging by removing or inhibiting free radicals, and has the effects of repairing skin damage and improving skin.

[0012] Polygonum cuspidatum root extract: This is a substance extracted from the root of Polygonum cuspidatum. It has a good inhibitory effect on the activity of B-16 melanocytes and a promoting effect on macrophage activity, thereby reducing melanin content from these two aspects. In addition, Polygonum cuspidatum root extract also has antioxidant properties and can play an anti-wrinkle and skin-rejuvenating role.

[0013] Scutellaria root extract: It is a mixture obtained by a series of extraction, purification and refining of dried Scutellaria rhizome. It contains baicalin, baicalin, wogonin and baicalin, and the active ingredients contained are relatively balanced. The four active ingredients work together to relieve and anti-oxidation effects, eliminate free radicals, thereby increasing skin metabolism, increasing skin elasticity and wrinkle resistance, achieving the purpose of anti-aging, and is beneficial to the repair and firming of the skin of the reproductive system. Scutellaria root extract can also promote the production of ceramide, improve sebum, and make the skin soft and smooth. In addition, Scutellaria root extract can also inhibit dermatitis, enhance skin resistance, and has anti-inflammatory properties, achieving the purpose of treating gynecological inflammations such as vaginitis and cervicitis. Scutellaria root extract can also neutralize the released histamine and has the ability to prevent allergies.

[0014] Tea extract: contains active substances such as tea polyphenols, which can scavenge active oxygen free radicals, have anti-aging effects, and can delay skin aging. At the same time, the antioxidant effect of tea polyphenols helps to reduce skin aging and achieve the purpose of firming the skin.

[0015] White Anthemis extract: White Anthemis, also known as Roman chamomile, is a plant of the genus Anthemis. White Anthemis extract is a mixture of triterpenoids extracted from white Anthemis. Triterpenoids can enhance the resistance of capillaries and inhibit capillary permeability, thus having anti-inflammatory effects, and can further treat reproductive system inflammations such as vaginitis and cervicitis, and have an inhibitory effect on induced skin allergies. At the same time, white Anthemis extract can eliminate free radicals, has antioxidant properties, and helps alleviate skin aging.

[0016] Elastic collagen: It has low immunogenicity, compatibility, degradability and coagulability. It can enhance blood vessel regeneration and form more collagen, thereby delaying the decline and physiological aging of the skin caused by the external environment, and achieving the purpose of tightening the skin of the reproductive system.

[0017] Arginine: It has the functions of moisturizing, solubilizing and regulating pH value.

[0018] Pentylene glycol, butylene glycol and glycerin are all moisturizing ingredients, which have the functions of moisturizing, lubricating, solubilizing and penetrating. Parahydroxyacetophenone and caprylhydroxamic acid have the functions of moisturizing, preserving and anti-inflammatory.

[0019] In the present application, the raw materials for preparing the firming and repairing gynecological gel include, by weight: 0.3-2 parts of carbomer, 0.1-2 parts of sodium hyaluronate, 2-10 parts of animal umbilical cord extract, 0.1-2 parts of madecassoside, 0.1-2 parts of carnosine, 0.5-1.5 parts of Polygonum cuspidatum root extract, 0.2-1 parts of Scutellaria baicalensis root extract, 0.3-3 parts of tea extract, 0.1-1.5 parts of white flower anthemum extract, 0.1-1.5 parts of elastic collagen, 0.3-2 parts of arginine, 1-8 parts of pentanediol, 3-10 parts of butylene glycol, 3-10 parts of glycerol, 0.2-2 parts of p-hydroxyacetophenone, 0.5-3 parts of caprylhydroxamic acid, and the rest is purified water.

[0020] Preferably, the raw materials for preparing the firming and repairing gynecological gel include, by weight: 0.5-1.5 parts of carbomer, 0.2-0.8 parts of sodium hyaluronate, 2-6 parts of animal umbilical cord extract, 0.2-1 parts of madecassoside, 0.2-1 parts of carnosine, 0.5-1 parts of polygonum cuspidatum root extract, 0.2-1 parts of scutellaria root extract, 1-2 parts of tea extract, 0.1-1 parts of white anthemum officinale extract, 0.5-1.0 parts of elastic collagen, 0.5-1.5 parts of arginine, 2-5 parts of pentanediol, 2-8 parts of butylene glycol, 5-10 parts of glycerol, 0.5-1 parts of p-hydroxyacetophenone, 0.8-1.5 parts of caprylhydroxamic acid, and the rest is purified water.

[0021] The present application also provides a method for preparing a firming and repairing gynecological gel, the method comprising: S01: In a clean operating environment, carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone are added to purified water for pre-dispersion, and heated to 80-85°C for dissolution to obtain a carbomer matrix; wherein, the amount of purified water added is based on the complete dissolution of carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone.

[0022] S02: Arginine is pre-dispersed with purified water and then added to the carbomer matrix, and stirred evenly to obtain a mixed solution a; wherein the amount of purified water added is based on the complete dissolution of arginine.

[0023] S03: adding animal umbilical cord extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Anthemis nobilis extract, elastic collagen, and caprylhydroxamic acid to the mixed solution a, and stirring evenly at a stirring speed of 150-300 rpm / min for 1-3 minutes to obtain a mixed solution b; S04: Add madecassoside and carnosine to the mixed solution b, stir evenly for 15-20 minutes at a stirring speed of 200-500 rpm / min, let it stand to remove bubbles, and obtain a gynecological gel.

[0024] The final viscosity of the gynecological gel prepared according to the above-mentioned raw materials and preparation method is 20000-80000 cps.

[0025] The present invention has the following beneficial effects: (1) Sodium hyaluronate has moisturizing and lubricating properties, increases the self-cleaning and lubricating effect of the vagina, promotes the proliferation and differentiation of epidermal cells, removes oxygen free radicals, and restores local epithelial cells. Animal umbilical cord extract can play an anti-inflammatory, repairing and regenerative role, and can also maintain cell stability, transmit rejuvenation cell signals to skin cells, delay skin aging, and achieve the effect of repairing and firming. Madecassoside has anti-inflammatory, anti-ulcer and antioxidant activities, can repair skin damage and scars, promote fibroblast regeneration, accelerate wound healing, and can effectively repair skin ulcers caused by inflammation such as vaginitis and cervicitis, and accelerate wound healing. The active ingredients in Polygonum cuspidatum root extract, Scutellaria baicalensis root extract and tea extract have antioxidant and anti-inflammatory properties, can increase skin elasticity and anti-wrinkle properties, achieve the purpose of anti-aging and treatment of gynecological inflammations such as vaginitis and cervicitis, and are beneficial to the repair and firming of the reproductive system skin. In addition, through the coordination of the various components in the preparation of raw materials, the skin affinity of the gel can be greatly improved, and it can effectively replenish moisture and nutrients to the skin, which helps to improve the quality of skin cells.

[0026] (2) The gynecological gel can maintain a balanced bacterial population and keep the vaginal microenvironment normal; improve vaginal atrophy, restore vaginal firmness, and relieve vaginal dryness; it can also repair damaged mucosa, strengthen women’s natural defense function, and prevent gynecological infections.

[0027] (3) This gynecological gel has excellent biocompatibility, cell affinity and mechanical properties. It can supplement collagen to the female vulva, repair damaged vaginal mucosa and promote wound healing. It can also moisturize the vagina, improve vaginal relaxation and dryness, regulate the balance of vaginal microecology and enhance the immunity of the female reproductive system. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a diagram of the TNF-α test results during the repair performance test; Figure 2 This is a diagram of the IL-6 test results during the repair performance test; Figure 3 This is a microscope image of the 3D skin model of the blank control group during the firming performance test; Figure 4 This is a microscope image of the 3D skin model of the negative control group during the firming performance test; Figure 5 This is a microscope image of the 3D skin model of the positive control group during the firming performance test; Figure 6Microscope image of the 3D skin model of the sample group during the firming performance test. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further explained and illustrated by means of specific embodiments below.

[0030] Example 1 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 0.8 parts of carbomer, 0.3 parts of sodium hyaluronate, 5 parts of animal umbilical cord extract, 0.6 parts of madecassoside, 0.5 parts of carnosine, 0.5 parts of polygonum cuspidatum root extract, 0.5 parts of scutellaria baicalensis root extract, 1 part of tea extract, 0.8 parts of anthemis nobilis extract, 0.8 parts of elastin collagen, 0.8 parts of arginine, 3 parts of pentanediol, 6 parts of butylene glycol, 6 parts of glycerol, 0.5 parts of p-hydroxyacetophenone, 1 part of caprylhydroxamic acid, and the remainder is purified water.

[0031] The preparation method of the firming and repairing gynecological gel provided in the embodiment of the present application comprises: S101: In a clean operating environment, carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone are added to purified water for pre-dispersion, and heated to 80°C for dissolution to obtain a carbomer matrix; wherein the amount of purified water added is based on the complete dissolution of carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone.

[0032] S102: After arginine is pre-dispersed with purified water, it is added to the carbomer matrix and stirred evenly to obtain a mixed solution a; wherein the amount of purified water added is based on the complete dissolution of arginine.

[0033] S103: adding animal umbilical cord extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Anthemis nobilis extract, elastic collagen, and caprylhydroxamic acid to the mixed solution a, and stirring the mixture at a stirring speed of 200 rpm / min for 2 minutes to obtain a mixed solution b; S104: Add madecassoside and carnosine to the mixed solution b, stir evenly for 18 minutes at a stirring speed of 350 rpm / min, let stand to remove bubbles, and obtain a gynecological gel.

[0034] Example 2 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 0.5 parts of carbomer, 0.3 parts of sodium hyaluronate, 3 parts of animal umbilical cord extract, 0.5 parts of madecassoside, 0.6 parts of carnosine, 0.8 parts of polygonum cuspidatum root extract, 0.3 parts of scutellaria root extract, 1.2 parts of tea extract, 0.5 parts of anthemis nobilis extract, 0.8 parts of elastic collagen, 0.5 parts of arginine, 5 parts of pentanediol, 3 parts of butylene glycol, 5 parts of glycerol, 0.8 parts of p-hydroxyacetophenone, 0.8 parts of caprylhydroxamic acid, and the remainder is purified water.

[0035] The preparation method of the firming and repairing gynecological gel provided in the embodiment of the present application comprises: S201: In a clean operating environment, carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone are added to purified water for pre-dispersion, and heated to 82°C for dissolution to obtain a carbomer matrix; wherein the amount of purified water added is based on the complete dissolution of carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone.

[0036] S202: After arginine is pre-dispersed with purified water, it is added to the carbomer matrix and stirred evenly to obtain a mixed solution a; wherein the amount of purified water added is based on the complete dissolution of arginine.

[0037] S203: adding animal umbilical cord extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Anthemis nobilis extract, elastic collagen, and caprylhydroxamic acid to the mixed solution a, and stirring evenly at a stirring speed of 150 rpm / min for 3 minutes to obtain a mixed solution b; S204: Add madecassoside and carnosine to the mixed solution b, stir evenly for 20 minutes at a stirring speed of 200 rpm / min, let it stand to remove bubbles, and obtain a gynecological gel.

[0038] Example 3 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 0.5 parts of carbomer, 0.5 parts of sodium hyaluronate, 5 parts of animal umbilical cord extract, 0.8 parts of madecassoside, 1 part of carnosine, 0.5 parts of Polygonum cuspidatum root extract, 0.8 parts of Scutellaria baicalensis root extract, 1 part of tea extract, 0.8 parts of Anthemis nobilis extract, 1 part of elastin collagen, 0.5 parts of arginine, 3 parts of pentanediol, 6 parts of butylene glycol, 5 parts of glycerol, 1 part of p-hydroxyacetophenone, 1 part of caprylhydroxamic acid, and the remainder is purified water.

[0039] The preparation method of the firming and repairing gynecological gel provided in the embodiment of the present application comprises: S301: In a clean operating environment, carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone are added to purified water for pre-dispersion, and heated to 85°C for dissolution to obtain a carbomer matrix; wherein the amount of purified water added is based on the complete dissolution of carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerol and p-hydroxyacetophenone.

[0040] S302: After arginine is pre-dispersed with purified water, it is added to the carbomer matrix and stirred evenly to obtain a mixed solution a; wherein the amount of purified water added is based on the complete dissolution of arginine.

[0041] S303: adding animal umbilical cord extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Anthemis nobilis extract, elastic collagen, and caprylhydroxamic acid to the mixed solution a, and stirring evenly at a stirring speed of 300 rpm / min for 1 min to obtain a mixed solution b; S304: Add madecassoside and carnosine to the mixed solution b, stir evenly for 15 minutes at a stirring speed of 500 rpm / min, let it stand to remove bubbles, and obtain a gynecological gel.

[0042] Example 4 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 1 part of carbomer, 0.2 parts of sodium hyaluronate, 5 parts of animal umbilical cord extract, 0.8 parts of madecassoside, 1 part of carnosine, 0.5 parts of Polygonum cuspidatum root extract, 0.8 parts of Scutellaria baicalensis root extract, 1 part of tea extract, 0.8 parts of Anthemis nobilis extract, 1 part of elastic collagen, 1 part of arginine, 3 parts of pentanediol, 6 parts of butylene glycol, 5 parts of glycerol, 1 part of p-hydroxyacetophenone, 1 part of caprylhydroxamic acid, and the rest is purified water.

[0043] The preparation method of the firming and repairing gynecological gel provided in the examples of the present application is the same as that in Example 1.

[0044] Example 5 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 0.7 parts of carbomer, 0.3 parts of sodium hyaluronate, 5 parts of animal umbilical cord extract, 1 part of madecassoside, 0.5 parts of carnosine, 1 part of Polygonum cuspidatum root extract, 0.5 parts of Scutellaria baicalensis root extract, 1.2 parts of tea extract, 1 part of Anthemis nobilis extract, 1 part of elastin collagen, 0.7 parts of arginine, 2 parts of pentanediol, 5 parts of butylene glycol, 5 parts of glycerol, 0.5 parts of p-hydroxyacetophenone, 0.5 parts of caprylhydroxamic acid, and the remainder is purified water.

[0045] The preparation method of the firming and repairing gynecological gel provided in the examples of the present application is the same as that in Example 1.

[0046] Example 6 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 0.8 parts of carbomer, 0.3 parts of sodium hyaluronate, 6 parts of animal umbilical cord extract, 1 part of madecassoside, 1 part of carnosine, 0.5 parts of Polygonum cuspidatum root extract, 1 part of Scutellaria baicalensis root extract, 1.5 parts of tea extract, 1 part of Anthemis nobilis extract, 0.8 parts of elastic collagen, 0.8 parts of arginine, 3 parts of pentanediol, 4 parts of butylene glycol, 5 parts of glycerol, 0.5 parts of p-hydroxyacetophenone, 0.8 parts of caprylhydroxamic acid, and the rest is purified water.

[0047] The preparation method of the firming and repairing gynecological gel provided in the examples of the present application is the same as that in Example 1.

[0048] Example 7 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 0.3 parts of carbomer, 0.1 parts of sodium hyaluronate, 10 parts of animal umbilical cord extract, 0.1 parts of madecassoside, 0.1 parts of carnosine, 1.5 parts of Polygonum cuspidatum root extract, 0.2 parts of Scutellaria baicalensis root extract, 0.3 parts of tea extract, 0.1 parts of Anthemis nobilis extract, 0.1 parts of elastin collagen, 2 parts of arginine, 1 part of pentanediol, 10 parts of butylene glycol, 3 parts of glycerol, 0.2 parts of p-hydroxyacetophenone, 3 parts of caprylhydroxamic acid, and the remainder is purified water.

[0049] The preparation method of the firming and repairing gynecological gel provided in the examples of the present application is the same as that in Example 1.

[0050] Example 8 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 2 parts of carbomer, 2 parts of sodium hyaluronate, 2 parts of animal umbilical cord extract, 2 parts of madecassoside, 2 parts of carnosine, 0.8 parts of Polygonum cuspidatum root extract, 0.2 parts of Scutellaria baicalensis root extract, 3 parts of tea extract, 1.5 parts of Anthemis nobilis extract, 1.5 parts of elastin collagen, 0.3 parts of arginine, 8 parts of pentanediol, 2 parts of butylene glycol, 10 parts of glycerol, 2 parts of p-hydroxyacetophenone, 1.5 parts of caprylhydroxamic acid, and the remainder is purified water.

[0051] The preparation method of the firming and repairing gynecological gel provided in the examples of the present application is the same as that in Example 1.

[0052] Example 9 The embodiment of the present application provides a firming and repairing gynecological gel, and the raw materials for its preparation include, in parts by weight: 1.5 parts of carbomer, 0.8 parts of sodium hyaluronate, 4 parts of animal umbilical cord extract, 0.2 parts of madecassoside, 0.2 parts of carnosine, 0.8 parts of Polygonum cuspidatum root extract, 0.8 parts of Scutellaria baicalensis root extract, 2 parts of tea extract, 0.1 parts of Anthemis nobilis extract, 0.5 parts of elastin collagen, 1.5 parts of arginine, 3 parts of pentanediol, 8 parts of butylene glycol, 9 parts of glycerol, 0.8 parts of p-hydroxyacetophenone, 1.2 parts of caprylhydroxamic acid, and the remainder is purified water.

[0053] The preparation method of the firming and repairing gynecological gel provided in the examples of the present application is the same as that in Example 1.

[0054] The examples of the present application also experimentally test the stability, vaginal mucosal irritation, repair performance and firming performance of the prepared firming and repairing gynecological gel, and the experimental test processes are described in detail as follows.

[0055] 1. Stability test The firming and repairing gynecological gel prepared in Examples 1-9 was placed in a hot and cold cycle incubator at -18°C, 4°C, 40°C and 45°C, respectively, wherein one cycle was 12 hours at -18°C, 12 hours at 4°C, 12 hours at 40°C and 12 hours at 45°C. During the placement process, at the end of 1 week, 2 weeks, 4 weeks, 8 weeks and 12 weeks, samples were taken out from each temperature to observe whether the samples were abnormal. At any of the above times and temperatures, if the product shows precipitation, stratification, discoloration, taste change, and abnormal viscosity changes, and the abnormal change is more than 50% compared with the initial value, any abnormal situation will be recorded as "failure", and the others will be recorded as "normal". The test results show that the stability test results of Examples 1-9 are all normal.

[0056] 2. Vaginal mucosal irritation test According to 2.3.5 of the Technical Specification for Disinfection (2002 Edition), 6 rabbits with no abnormalities in the vaginal opening before the test were randomly divided into two groups, the poisoning group and the control group, with 3 rabbits in each group. The rabbits in the poisoning group were fixed on their backs, exposing the perineum and vaginal opening, and the catheter was gently inserted into the vagina 4-5cm after moistening the firming and repairing gynecological gel prepared in Example 5, and the firming and repairing gynecological gel prepared in Example 5 was slowly injected with a syringe, and the catheter was pulled out to complete the poisoning. The rabbits in the control group were treated in the same way with physiological saline. 24h after the poisoning, the animals were killed by gas embolism, the complete vagina was removed by laparotomy, and it was cut longitudinally. The naked eye was observed for congestion, edema and other manifestations for reference when taking pathological materials. The vagina was then placed in a 10% formalin solution and fixed for more than 24h, and tissue preparations were selected at both ends and 3 central parts of the vagina, and HE staining was performed to obtain HE staining pictures. Determine the irritation response score of each part of each rabbit based on the HE staining images and the content of 2.3.5 of the "Technical Specifications for Disinfection" (2002 edition).

[0057] The irritation reaction scores of the three parts of the rabbits in the poisoned group and the control group were added together and then divided by the total number of observations (number of animals × 3) to obtain the average score of the vaginal mucosal irritation reaction of the poisoned group and the control group. The irritation index was obtained by subtracting the average score of the control group from the average score of the poisoned group, as shown in Table 1. Among them, the irritation reaction score in Table 1 is the sum of the irritation reaction scores of the three parts of the vagina of each rabbit.

[0058] Then, the irritation intensity is graded according to the vaginal mucosal irritation intensity grading table. For the vaginal mucosal irritation intensity grading table, please refer to the "Disinfection Technical Specifications" (2002 edition).

[0059] Table 1: Irritation response scores of the poisoned group and the control group As shown in Table 1, compared with the control group, the average irritation index of the vaginal mucosal irritation test in the poisoned group was 2.67. According to the vaginal mucosal irritation intensity grading table in the "Technical Specifications for Disinfection" (2002 edition), the vaginal mucosal irritation in the poisoned group was extremely mild. This shows that the firming and repairing gynecological gel prepared in the examples of the present application has extremely weak irritation to the vagina.

[0060] 3. Repair performance test In the example of the present application, the test is divided into a blank control group BC, a negative control group NC, a positive control group PC and a sample group, wherein the blank control group BC is PBS (phosphate buffered saline); the negative control group NC is SLS (sodium lauryl sulfate) with a final concentration of 2 mg / mL; the positive control group PC is a 0.1% dexamethasone solution; and the sample group is the firming and repairing gynecological gel prepared in Example 5 of the present application.

[0061] 25 μL of the mixed solution was added to the surface of the 3D epidermal model in each group, and the model was gently shaken to evenly distribute the mixed solution on the surface of the model, and then placed in an incubator at 37°C and 5% CO2 for 24 hours. After the incubation, the 3D epidermal model culture solution was collected using an EP tube (eppendorf, centrifuge tube) and stored in a -80°C refrigerator. At the same time, the residual test substance on the surface of the 3D epidermal model was cleaned with a cleaning bottle containing sterile PBS solution, and the residual liquid inside and outside the model was gently wiped off with a sterile cotton swab.

[0062] The ELISA test kit containing TNF-α and IL-6 inflammatory factors was used to test the 3D epidermal model culture medium, and the test results were shown in Tables 2 and 3. GraphPad Prism Program software was used to draw a graph based on the test results, and the attached Figure 1 , 2 , wherein t-test statistical analysis was used between the groups. When the blank control group BC was compared with the negative control group NC, the significance was indicated by #, #p-value<0.05 indicated a significant difference, and ##p-value<0.01 indicated an extremely significant difference; when the negative control group NC, the positive control group PC and the sample group-Example 5 were compared, the significance was indicated by *, *p<0.05 indicated a significant difference, and **p<0.01 indicated an extremely significant difference.

[0063] Table 2: TNF-α detection results Table 3: IL-6 detection results From Tables 2, 3 and Appendix Figure 1 , 2 It can be seen that compared with the blank control group BC, the secretion levels of TNF-α and IL-6 in the negative control group NC were significantly increased (p<0.01), which shows that SLS stimulation can significantly increase the secretion of TNF-α and IL-6, indicating that the SLS stimulation conditions in this experiment are effective.

[0064] Compared with the negative control group NC group, the positive control group PC group was able to significantly inhibit the secretion of TNF-α and IL-6 (p<0.01), which indicated that the positive control test was effective.

[0065] Compared with the positive control group PC group, the sample group can inhibit the release of TNF-α and IL-6, and can repair barrier damage by repairing cell damage and inhibiting skin inflammatory response.

[0066] 4. Compactness performance test After UVA irradiation of the skin, the skin tissue structure will be damaged, and the content of collagen, elastin and hyaluronic acid will also decrease. In the examples of the present application, different groups of solutions are used to treat the irradiated 3D skin model, and the tissue morphology of the 3D skin model is detected to evaluate the skin tightening effect of the example samples.

[0067] Specifically, the three groups of 3D skin models were placed in a room with an irradiation dose of 50 J / cm 2 Irradiate under UVA lamp for 30 minutes. After the irradiation, take 100 μL of PBS solution and add it to the surface of the unirradiated 3D skin model to form a blank control group; take 100 μL of vitamin C with a concentration of 50 μg / mL and the firming and repairing gynecological gel prepared in Example 5 of the present application and add them to the surface of the irradiated 3D skin model to form a positive control group and a sample group. At the same time, the 3D skin model that has not been treated with solution and has been irradiated is a negative control group. Gently shake the 3D skin model so that each group of solutions is evenly distributed on the surface of the 3D skin model, and then place it in an incubator at 37°C and 5% CO2 for incubation for 3 days. During the incubation process, fresh culture medium is replaced every other day, and each group of solutions is added at the same time. After the incubation is completed, the residual test substance on the surface of the 3D skin model is cleaned with a cleaning bottle containing sterile PBS solution, and the residual liquid inside and outside the model is gently wiped off with a sterile cotton swab. Then, the 3D skin model is fixed with 4% paraformaldehyde for 24 hours. After fixation, the 3D skin model was removed by circumcision and HE staining, and then observed under a microscope to obtain the attached Figure 3-6 .

[0068] Compare with Figure 3 , 4 It can be seen that compared with the 3D skin model of the blank control group that was not irradiated, the epidermal living cell layer of the 3D skin model of the negative control group that was irradiated became significantly thinner and the cell density of the dermis layer decreased, which indicates that the irradiation stimulation conditions were effective.

[0069] Compare with Figure 4 , 5It can be seen that compared with the 3D skin model of the negative control group treated with irradiation, the epidermal living cell layer of the 3D skin model of the positive control group treated with vitamin C was thickened and the dermal cell density increased, indicating that the vitamin C solution can stimulate the growth of skin epidermal cells.

[0070] Compare with Figure 5 , 6 It can be seen that compared with the positive control group treated with vitamin C solution, the epidermal living cell layer of the 3D skin model of the sample group treated with the firming and repairing gynecological gel in Example 5 was significantly thickened, the tissue morphology was significantly improved, and the improvement effect was better than that of the positive control group. This shows that the firming and repairing gynecological gel prepared in Example 5 of the present application can significantly improve the tissue morphology of damaged skin, and the effect is good, achieving a firming effect on the skin.

[0071] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A firming and repairing gynecological gel, characterized in that: The preparation raw materials include: carbomer, sodium hyaluronate, animal umbilical cord extract, madecassoside, carnosine, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Anthemis nobilis extract, elastic collagen, arginine, pentylene glycol, butylene glycol, glycerin, p-hydroxyacetophenone, caprylhydroxamic acid and purified water.

2. The firming and repairing gynecological gel according to claim 1, characterized in that: The preparation raw materials include, by weight: 0.3-2 parts of carbomer, 0.1-2 parts of sodium hyaluronate, 2-10 parts of animal umbilical cord extract, 0.1-2 parts of madecassoside, 0.1-2 parts of carnosine, 0.5-1.5 parts of polygonum cuspidatum root extract, 0.2-1 parts of scutellaria root extract, 0.3-3 parts of tea extract, 0.1-1.5 parts of white flower anthemis nobilis extract, 0.1-1.5 parts of elastin collagen, 0.3-2 parts of arginine, 1-8 parts of pentanediol, 3-10 parts of butylene glycol, 3-10 parts of glycerol, 0.2-2 parts of p-hydroxyacetophenone, 0.5-3 parts of octanoylhydroxamic acid, and the rest is purified water.

3. The firming and repairing gynecological gel according to claim 1, characterized in that: The preparation raw materials include, by weight: 0.5-1.5 parts of carbomer, 0.2-0.8 parts of sodium hyaluronate, 2-6 parts of animal umbilical cord extract, 0.2-1 parts of madecassoside, 0.2-1 parts of carnosine, 0.5-1 parts of polygonum cuspidatum root extract, 0.2-1 parts of scutellaria baicalensis root extract, 1-2 parts of tea extract, 0.1-1 parts of white flower anthemis nobilis extract, 0.5-1.0 parts of elastin collagen, 0.5-1.5 parts of arginine, 2-5 parts of pentanediol, 2-8 parts of butylene glycol, 5-10 parts of glycerol, 0.5-1 parts of p-hydroxyacetophenone, 0.8-1.5 parts of octanoylhydroxamic acid, and the rest is purified water.

4. The firming and repairing gynecological gel according to claim 1, characterized in that: The final viscosity of the gynecological gel is 20,000-80,000 cps.

5. A method for preparing a firming and repairing gynecological gel, characterized in that: include: Carbomer, sodium hyaluronate, pentylene glycol, butylene glycol, glycerin and p-hydroxyacetophenone are added into purified water for pre-dispersion and heated to dissolve to obtain a carbomer matrix; Arginine is pre-dispersed with purified water, and then added to the carbomer matrix, and stirred evenly to obtain a mixed solution a; Adding animal umbilical cord extract, Polygonum cuspidatum root extract, Scutellaria baicalensis root extract, tea extract, Anthemis nobilis extract, elastin collagen, and caprylhydroxamic acid to the mixed solution a, stirring evenly to obtain a mixed solution b; Add madecassoside and carnosine to the mixed solution b, stir evenly, and let stand to remove bubbles to obtain a gynecological gel.

6. The method for preparing the firming and repairing gynecological gel according to claim 5, characterized in that: The heating and dissolving temperature is 80-85°C.

7. The method for preparing the firming and repairing gynecological gel according to claim 5, characterized in that: The stirring speed of the mixed solution a to form the mixed solution b is 150-300 rpm / min, and the stirring time is 1-3 min.

8. The method for preparing the firming and repairing gynecological gel according to claim 5, characterized in that: The stirring speed of the mixed solution b when forming the gynecological gel is 200-500 rpm / min, and the stirring time is 15-20 min.

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