Water quality improver for aquaculture and preparation method thereof

Abstract

The invention relates to a water quality improver for aquaculture and a preparation method thereof, and the water quality improver for aquaculture is prepared from the following raw materials in percentage by weight: 1-10% of a lactic acid bacterium agent, 1-10% of a bacillus subtilis agent, 1-10% of a bacillus licheniformis agent, 10-30% of a photosynthetic bacterium agent and 50-70% of a carrier, wherein a lactobacillus plantarum LP45 bacterial agent is adopted as the lactic acid bacteria bacterial agent, and the lactobacillus plantarum LP45 bacterial agent contains lactobacillus plantarum LP45. The aquaculture water purifying agent can quickly and efficiently purify aquaculture water, and is low in cost, free of pollution and free of drug residues.

Description

Technical Field

[0001] The invention belongs to the technical field of aquaculture, and particularly relates to an aquaculture water quality improver and a preparation method thereof. Background Art

[0002] There is a saying in the industry that "to raise fish you must first raise water". This is because water is the basic living environment for aquatic animals. The quality of water directly affects the survival, growth and reproduction of farmed aquatic animals, restricts the orientation of aquaculture management technology, and thus affects the production performance and product quality of aquatic animals.

[0003] With the advancement of breeding technology, high-density breeding has become an industry trend. The number of fish in earthen ponds is 10,000-30,000 per mu, and in factory-based breeding, it is 600-700 per square meter. Problems brought about by high-density breeding also come along. The self-purification and regulation capabilities of the ponds cannot meet the requirements of removing residual bait, fish and shrimp feces and other factors. Ammonia nitrogen, nitrite, sulfide and other factors in the breeding water are seriously exceeded, the pond water quality deteriorates, and fish and shrimp diseases occur frequently.

[0004] In the current existing technology, the solution adopted to solve the above technical problems is usually to apply a large amount of antibiotics and various chemical drugs in the breeding ponds. The application of these antibiotics and chemical drugs seriously damages the biological system of the breeding water environment, leading to microecological imbalance in the breeding ponds, and serious drug residues pollute the breeding water environment. Therefore, the development of a pollution-free, residue-free aquaculture water quality improver and its preparation method is crucial for the efficient and low-cost purification of breeding water. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects in the prior art and provide a water quality improver for aquaculture and a preparation method thereof, which can quickly and efficiently purify aquaculture water and has low cost, no pollution and no drug residue.

[0006] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] A water quality improver for aquaculture, comprising the following raw materials in percentage by weight:

[0008] 1-10% lactic acid bacteria agent, 1-10% Bacillus subtilis agent, 1-10% Bacillus licheniformis agent, 10-30% photosynthetic bacteria agent and 50-70% carrier;

[0009] Wherein, the lactic acid bacteria agent adopts Lactobacillus plantarum LP45 agent, and the Lactobacillus plantarum LP45 agent contains Lactobacillus plantarum LP45.

[0010] As a further technical solution, a water quality improver for aquaculture includes the following raw materials in percentage by weight:

[0011] 1-9% lactic acid bacteria agent, 1-9% Bacillus subtilis agent, 1-9% Bacillus licheniformis agent, 10-25% photosynthetic bacteria agent and 55-70% carrier.

[0012] As a further technical solution, a water quality improver for aquaculture includes the following raw materials in percentage by weight:

[0013] 1% lactic acid bacteria agent, 3% Bacillus subtilis agent, 1% Bacillus licheniformis agent, 25% photosynthetic bacteria agent and 70% carrier.

[0014] As a further technical solution, the number of live bacteria in the lactic acid bacteria agent is greater than or equal to 1.12×10 8 cfu / g;

[0015] The number of live bacteria in the Bacillus subtilis inoculant is greater than or equal to 1.125×10 9 cfu / g;

[0016] The number of live bacteria in the Bacillus licheniformis inoculant is greater than or equal to 1.014×10 9 cfu / g;

[0017] The number of live bacteria in the photosynthetic bacterial agent is greater than or equal to 1.76×10 9 cfu / g.

[0018] As a further technical solution, the Lactobacillus plantarum LP45 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with the deposit number being CGMCC No.8072.

[0019] A method for preparing a water quality improver for aquaculture, comprising the following steps:

[0020] Step 1, sieving the carrier to obtain carrier particles with a diameter less than or equal to 2 mm;

[0021] Step 2: premix the microbial agent by weight using a three-dimensional motion mixer, and then evenly mix the premixed agent with the carrier particles to obtain a water quality improver for aquaculture.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention selects and optimizes microorganisms, and the microorganisms work together and cooperate with carriers to degrade ammonia nitrogen in aquaculture water. The degradation is fast, efficient, and has a long action time, which enhances the production performance of aquatic products and greatly improves the economic benefits of aquaculture. DETAILED DESCRIPTION

[0024] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] In addition, it should be noted that the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0026] In the present invention,

[0027] The deposit number of Lactobacillus plantarum LP45 is CGMCC No.8072, which is disclosed in the patent application with application number CN201310587738.8 and invention name “A Lactobacillus plantarum and its application”;

[0028] Bacillus subtilis inoculant was purchased from Qingdao Weilan Biotechnology Co., Ltd.

[0029] Bacillus licheniformis agent was purchased from Qingdao Weilan Biotechnology Co., Ltd.;

[0030] The photosynthetic bacterial agent used was Rhodopseudomonas palustris, which was purchased from Cangzhou Wangfa Biotechnology Research Institute Co., Ltd.

[0031] The carrier is medical stone.

[0032] Unless otherwise specified, the raw materials used in the present invention are commercially available.

[0033] Embodiment 1:

[0034] A water quality improver for aquaculture, comprising the following raw materials in percentage by weight:

[0035] 1% lactic acid bacteria agent, 3% Bacillus subtilis agent, 1% Bacillus licheniformis agent, 25% photosynthetic bacteria agent and 70% carrier;

[0036] Wherein, the lactic acid bacteria agent adopts Lactobacillus plantarum LP45 agent, and the Lactobacillus plantarum LP45 agent contains Lactobacillus plantarum LP45;

[0037] The number of live bacteria in the lactic acid bacteria agent is 1.12×10 8 cfu / g;

[0038] The number of live bacteria in the Bacillus subtilis inoculant is 1.125×109 cfu / g;

[0039] The number of live bacteria in the Bacillus licheniformis agent is 1.014×10 9 cfu / g;

[0040] The number of live bacteria in the photosynthetic bacterial agent is 2.2×10 9 cfu / g.

[0041] The preparation method thereof comprises the following steps:

[0042] Step 1, sieving the carrier to obtain carrier particles with a diameter less than or equal to 2 mm;

[0043] Step 2: premix the microbial agent by weight using a three-dimensional motion mixer, and then evenly mix the premixed agent with the carrier particles to obtain a water quality improver for aquaculture.

[0044] Example 2

[0045] A water quality improver for aquaculture, comprising the following raw materials in percentage by weight:

[0046] 5% lactic acid bacteria agent, 3% Bacillus subtilis agent, 2% Bacillus licheniformis agent, 20% photosynthetic bacteria agent and 70% carrier;

[0047] Wherein, the lactic acid bacteria agent adopts Lactobacillus plantarum LP45 agent, and the Lactobacillus plantarum LP45 agent contains Lactobacillus plantarum LP45;

[0048] The number of live bacteria in the lactic acid bacteria agent is 5.6×10 8 cfu / g;

[0049] The number of live bacteria in the Bacillus subtilis inoculant is 1.125×10 9 cfu / g;

[0050] The number of live bacteria in the Bacillus licheniformis inoculant is 2.028×10 9 cfu / g;

[0051] The number of live bacteria in the photosynthetic bacterial agent is 1.76×10 9 cfu / g.

[0052] The preparation method is the same as that in Example 1.

[0053] Example 3

[0054] A water quality improver for aquaculture, comprising the following raw materials in percentage by weight:

[0055] 2% lactic acid bacteria agent, 6% Bacillus subtilis agent, 3% Bacillus licheniformis agent, 25% photosynthetic bacteria agent and 64% carrier;

[0056] Wherein, the lactic acid bacteria agent adopts Lactobacillus plantarum LP45 agent, and the Lactobacillus plantarum LP45 agent contains Lactobacillus plantarum LP45;

[0057] The number of live bacteria in the lactic acid bacteria agent is 2.24×10 8 cfu / g;

[0058] The number of live bacteria in the Bacillus subtilis inoculant is 6.75×10 9 cfu / g;

[0059] The number of live bacteria in the Bacillus licheniformis inoculant is 3.042×10 9 cfu / g;

[0060] The number of live bacteria in the photosynthetic bacterial agent is 2.2×10 9 cfu / g.

[0061] The preparation method is the same as that in Example 1.

[0062] Application effect example 1:

[0063] 1. Test methods:

[0064] (1) Experimental animals

[0065] Mr. Liu in Jing'an Town, Zhuhai has two ponds covering a total area of ​​16 mu, with each pond being about 8 mu and the water depth of the pond being 1.3 meters. On March 10, the shrimps in both ponds began to eat slower, with a serious problem of leftover feed. Some shrimps even died. Testing of the pond water quality revealed that the ammonia nitrogen content exceeded the standard.

[0066] (2) Trial groups and medication

[0067] Test group: 10 kg of the aquaculture water quality improver in Example 1 of the present invention was used, brown sugar and water were added, activated for two hours in a ratio of 2:1:20, and then sprayed throughout the pond.

[0068] Control group: Maintain normal feeding

[0069] Farming management: The operation of the aerator and the feeding amount were kept consistent. The results are shown in Table 1.

[0070] 2. Test results:

[0071] Table 1 Effect of aquaculture water quality improvers on ammonia nitrogen content in aquaculture ponds

[0072]

[0073] Application effect example 2

[0074] 1. Test methods

[0075] Mr. Liu from Baijiao Town, Zhuhai, raised three white shrimps. Each pond had 10 mu and an average water depth of 1.5 meters. The ammonia nitrogen content in pond No. 3 was 0.4, which was selected as the experimental group. The ammonia nitrogen content in pond No. 4 was 0.4, which was selected as the control group.

[0076] 2. Trial Grouping and Medication

[0077] Experimental group: keep normal feeding + 15kg of aquaculture water quality improver of Example 2 of the present invention, add water and brown sugar in a ratio of 2:1:40, activate for 2 hours in the morning, and spray the whole pond at around 10 o'clock;

[0078] Control group: maintained normal feeding;

[0079] Experimental diet and feeding management: Same as effect example 1; results are shown in Table 2;

[0080] 3. Test results:

[0081] Table 2 Effect of aquaculture water quality improvers on ammonia nitrogen content in aquaculture ponds

[0082]

[0083] Application effect example 3

[0084] 1. Test methods

[0085] Mr. Yang from Dongtai, Jiangsu, raises yellow croaker in two ponds. Ponds 1 and 2 are 10 mu each and have a water depth of 1.7 meters. On April 8, the ammonia nitrogen content exceeded the standard. Pond 1 was the test group and Pond 2 was the control group.

[0086] 2. Grouping

[0087] Experimental group: keep normal feeding + 10 kg of the aquaculture water quality improver of Example 3 of the present invention, add water and brown sugar, activate for 2 hours according to the ratio of 2:1:40, and spray throughout the pond.

[0088] Control group: Maintain normal feeding

[0089] Experimental diet and feeding management: same as in effect example 1; results are shown in Table 3.

[0090] 3. Test results:

[0091] Table 3 Effect of aquaculture water quality improvers on ammonia nitrogen content in aquaculture ponds

[0092]

[0093] The above-described embodiments are only preferred embodiments of the present invention, and are not exhaustive of the feasible implementations of the present invention. For those skilled in the art, any obvious changes made thereto without departing from the principles and spirit of the present invention should be considered to be included in the scope of protection of the claims of the present invention.

Claims

1. A water quality improver for aquaculture, characterized in that: The following raw materials are included in percentage by weight: 1-10% lactic acid bacteria agent, 1-10% Bacillus subtilis agent, 1-10% Bacillus licheniformis agent, 10-30% photosynthetic bacteria agent and 50-70% carrier; Wherein, the lactic acid bacteria agent adopts Lactobacillus plantarum LP45 agent, and the Lactobacillus plantarum LP45 agent contains Lactobacillus plantarum LP45.

2. The water quality improver for aquaculture according to claim 1, characterized in that: The following raw materials are included in percentage by weight: 1-9% lactic acid bacteria agent, 1-9% Bacillus subtilis agent, 1-9% Bacillus licheniformis agent, 10-25% photosynthetic bacteria agent and 55-70% carrier.

3. The water quality improver for aquaculture according to claim 1, characterized in that: The following raw materials are included in percentage by weight: 1% lactic acid bacteria agent, 3% Bacillus subtilis agent, 1% Bacillus licheniformis agent, 25% photosynthetic bacteria agent and 70% carrier.

4. A water quality improver for aquaculture according to any one of claims 1 to 3, characterized in that: The number of live bacteria in the lactic acid bacteria agent is greater than or equal to 1.12×10 8 cfu / g; The number of live bacteria in the Bacillus subtilis inoculant is greater than or equal to 1.125×10 9 cfu / g; The number of live bacteria in the Bacillus licheniformis inoculant is greater than or equal to 1.014×10 9 cfu / g; The number of live bacteria in the photosynthetic bacterial agent is greater than or equal to 1.76×10 9 cfu / g.

5. The water quality improver for aquaculture according to any one of claims 1 to 3, characterized in that: The Lactobacillus plantarum LP45 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, with the deposit number being CGMCC No.8072.

6. A method for preparing a water quality improver for aquaculture, characterized in that: The following steps are involved: Step 1, sieving the carrier to obtain carrier particles with a diameter less than or equal to 2 mm; Step 2: premix the microbial agent by weight using a three-dimensional motion mixer, and then evenly mix the premixed agent with the carrier particles to obtain a water quality improver for aquaculture.

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