Tissue treatment device for protein purification

Abstract

The invention relates to the technical field of protein purification, in particular to a tissue treatment device for protein purification, which comprises a box body, the interior of the box body is divided into an upper cavity and a lower cavity by a transverse partition plate, and the upper cavity is divided into a feeding chamber and a sampling chamber for placing samples by a movable sealing plate; the lower cavity is divided into an equipment chamber and a treatment chamber for sampling treatment through a vertical partition plate, the end, penetrating through a transverse partition plate and extending into the equipment chamber, of a movable sealing plate is fixedly connected with the movable ends of a plurality of second electric telescopic rods, and a refrigerating machine for refrigerating the feeding chamber, the sampling chamber, the equipment chamber and the treatment chamber is arranged on one side of the box body. Ultraviolet emitters for sterilization are respectively mounted in the feeding chamber, the sampling chamber, the equipment chamber and the treatment chamber; a driving assembly is arranged at the top in the sampling chamber and is fixedly connected with a sampling assembly for sampling. According to the invention, a sample can be collected and sampled in a sterile and appropriate temperature environment; and the sampling and processing effects are effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of protein purification, and in particular to a tissue processing device for protein purification. Background Art

[0002] Protein separation and purification is an important technique used extensively in biochemical research applications. A typical eukaryotic cell can contain thousands of different proteins, some of which are very abundant and some of which contain only a few copies. In order to study a particular protein, it must first be purified from other proteins and non-protein molecules.

[0003] Most of the current existing technologies collect and sample samples manually, and manually peel off or extract part of the sample tissue from the sample through a sampling tool. Although the sampling of the sample tissue is carried out in a sterile laboratory, when the sample is taken out from the refrigerated condition for sample tissue collection and sampling, the process is long, resulting in bacterial spores in the air adhering to the surface of the sample, which will inevitably cause problems such as slight oxidation of the sample and protein denaturation, so that the protein finally purified is not the target protein; therefore, there is an urgent need for a tissue processing device for protein purification that can reduce the contact time between the sample and the air and can collect and sample the sample in a sterile environment and at a temperature suitable for preserving the sample. Summary of the invention

[0004] The purpose of the present invention is to provide a tissue processing device for protein purification to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above-mentioned purpose, the present invention provides the following scheme: a tissue processing device for protein purification, comprising a box body, wherein the box body is divided into an upper chamber and a lower chamber by a transverse partition, the upper chamber is divided into a feed chamber and a sampling chamber for placing samples by a movable sealing plate, and the lower chamber is divided into an equipment chamber and a processing chamber for sampling processing by a vertical partition, and one end of the movable sealing plate that penetrates through the transverse partition and extends into the equipment chamber is fixedly connected to the movable ends of a plurality of second electric telescopic rods, a refrigerator for cooling the feed chamber, the sampling chamber, the equipment chamber, and the processing chamber is provided on one side of the box body, and ultraviolet emitters for sterilization are respectively installed in the feed chamber, the sampling chamber, the equipment chamber, and the processing chamber;

[0006] A driving assembly is arranged on the top of the sampling chamber, and a sampling assembly for sampling is fixedly connected to the driving assembly.

[0007] Preferably, an observation window is embedded in the side of the box body facing away from the refrigerator, a first through groove connected to the feed chamber is opened on one side of the box body, a feed sealing door is installed in the first through groove, and a second through groove connected to the processing chamber is opened at the end of the box body facing away from the first through groove, and a discharge sealing door is installed in the second through groove.

[0008] Preferably, a control panel is provided above the discharge sealing door, and the control panel is embedded on the outer wall of the box body.

[0009] Preferably, a sampling dish is provided at the lower portion of the feed chamber, and the bottom surface of the sampling dish is fixedly connected to the rotating end of an electric turntable, and the fixed end of the electric turntable is fixedly connected to the movable end of a first electric telescopic rod, and the fixed end of the first electric telescopic rod passes through the transverse partition and extends into the equipment chamber, and the fixed end of the first electric telescopic rod is fixedly connected to the bottom of the equipment chamber.

[0010] Preferably, the driving assembly includes a screw, both ends of the screw are rotatably connected to fixed blocks, the fixed block is fixedly connected to the top of the sampling chamber, the screw passes through either end of the fixed block and is transmission-connected to a motor, the motor is fixedly connected to the fixed block, the screw is threadedly connected to a slider, one end of the slider facing the transverse partition is fixedly connected to the fixed end of a third electric telescopic rod, and the movable end of the third electric telescopic rod is fixedly connected to the sampling assembly.

[0011] Preferably, the sampling assembly includes a fixed end of a fourth electric telescopic rod fixedly connected to the movable end of the third electric telescopic rod, a fifth electric telescopic rod and a withdrawal tube are fixedly connected to the side wall of the movable end of the fourth electric telescopic rod, and the fifth electric telescopic rod is arranged above the withdrawal tube.

[0012] Preferably, a piston is fixedly connected to the movable end of the fifth electric telescopic rod, the piston extends into the extraction cylinder and is slidably connected to the extraction cylinder, and the extraction cylinder is fixedly connected to the center of one end of the diaphragm and is connected with a needle tube.

[0013] Preferably, a grinder is fixedly connected in the processing chamber, the grinder is arranged close to the vertical partition, the top of the grinder is fixedly connected and communicated with a feed pipe, one end of the feed pipe away from the grinder passes through the horizontal partition and extends into the sampling chamber, the lower part of the outer wall of the grinder is fixedly connected and communicated with a discharge pipe, the discharge pipe is connected to a centrifuge through a telescopic hose, and the centrifuge is fixedly connected in the processing chamber close to the discharge sealing door.

[0014] Preferably, the top surface of the centrifuge is lower than the bottom surface of the pulverizer.

[0015] Preferably, the top surface of the pulverizer is connected to a liquid inlet pipe, one end of the liquid inlet pipe away from the pulverizer passes through the observation window, and a silicone sealing plug is installed in the end of the liquid inlet pipe extending out of the observation window.

[0016] The present invention discloses the following technical effects:

[0017] The present invention can reduce the contact time of the sample with the outside air by directly placing the sample in the feeding room after taking it out from the cold storage room. At the same time, the temperature in the box can be made suitable for the storage temperature of the sample through the refrigerator, and the feeding room, sampling room, equipment room and processing room can be sterilized through the ultraviolet emitter, so that the sample can be collected and sampled in a sterile environment and at a temperature suitable for preserving the sample, effectively improving the sampling and processing effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 It is a schematic diagram of the side structure of the box body of the present invention;

[0021] Figure 3 It is a schematic diagram of the cross-sectional structure of the box body of the present invention;

[0022] Figure 4 This is a schematic diagram of the structure of the sampling assembly of the present invention;

[0023] Among them, 1. box body; 2. horizontal partition; 3. screw; 4. vertical partition; 5. refrigerator; 6. ultraviolet emitter; 11. observation window; 12. feeding sealing door; 13. discharging sealing door; 14. control panel; 21. first electric telescopic rod; 22. electric turntable; 23. sampling dish; 24. movable sealing plate; 25. second electric telescopic rod; 31. fixed block; 32. motor; 33. slider; 34. third electric telescopic rod; 35. fourth electric telescopic rod; 36. fifth electric telescopic rod; 37. extraction cylinder; 41. pulverizer; 42. telescopic hose; 43. centrifuge. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0026] Reference Figure 1-Figure 4 The present invention provides a tissue processing device for protein purification, comprising a box body 1, wherein the box body 1 is divided into an upper chamber and a lower chamber by a transverse partition 2, the upper chamber is divided into a feed chamber and a sampling chamber for placing samples by a movable sealing plate 24, and the lower chamber is divided into an equipment chamber and a processing chamber for sampling processing by a vertical partition, and one end of the movable sealing plate 24 extending through the transverse partition into the equipment chamber is fixedly connected to the movable ends of a plurality of second electric telescopic rods 25, a refrigerator 5 for cooling the feed chamber, the sampling chamber, the equipment chamber, and the processing chamber is provided on one side of the box body 1, and ultraviolet emitters 6 for sterilization are respectively installed in the feed chamber, the sampling chamber, the equipment chamber, and the processing chamber; a driving assembly is provided on the top of the sampling chamber, and the driving assembly is fixedly connected to the sampling assembly for sampling.

[0027] The present invention can reduce the time that the sample is in contact with the outside air by directly placing the sample in the feeding room after taking it out from the cold storage room. At the same time, the temperature in the box body 1 can be made suitable for the storage temperature of the sample through the refrigerator 5, and the feeding room, sampling room, equipment room and processing room can be sterilized through the ultraviolet emitter 6, so that the sample can be collected and sampled in a sterile environment and at a temperature suitable for preserving the sample, effectively improving the sampling and processing effects.

[0028] A further optimized solution is that an observation window 11 is embedded on the side of the box body 1 facing away from the refrigerator 5, a first through groove connected to the feed chamber is opened on one side of the box body 1, a feed sealing door 12 is installed in the first through groove, and a second through groove connected to the processing chamber is opened on the end of the box body 1 facing away from the first through groove, a discharge sealing door 13 is installed in the second through groove.

[0029] The refrigerator 5 can balance the temperature in the feed room, sampling room, equipment room and processing room, and make the temperature in the feed room, sampling room, equipment room and processing room suitable for the temperature of the sample, so as to keep the sample active.

[0030] The feeding sealing door 12 facilitates the placement of samples into the feeding chamber, and the feeding sealing door 12 can isolate the feeding chamber from the outside.

[0031] The discharged sealed door 13 facilitates the removal of the processed sample from the processing chamber, and the discharged sealed door 13 can isolate the discharged chamber from the outside.

[0032] The feed room, sampling room, equipment room and processing room can be observed through the observation window 11.

[0033] According to a further optimized solution, a control panel 14 is provided above the discharge sealing door 13 , and the control panel 14 is embedded in the outer wall of the box body 1 .

[0034] The refrigeration temperature of the refrigerator 5 is controlled by the control panel 14, and the ultraviolet emitter 6 is controlled by the control panel 14 to sterilize the feed room, the sampling room, the equipment room, and the processing room.

[0035] To further optimize the solution, a sampling dish 23 is provided at the lower part of the feed chamber, and the bottom surface of the sampling dish 23 is fixedly connected to the rotating end of the electric turntable 22, and the fixed end of the electric turntable 22 is fixedly connected to the movable end of the first electric telescopic rod 21, and the fixed end of the first electric telescopic rod 21 penetrates the transverse partition 2 and extends into the equipment chamber, and the fixed end of the first electric telescopic rod 21 is fixedly connected to the bottom of the equipment chamber.

[0036] The control panel 14 is electrically connected to the first electric telescopic rod 21 and the electric turntable 22 , and the operation of the first electric telescopic rod 21 and the electric turntable 22 is controlled by the control panel 14 .

[0037] The height of the sampling dish 23 can be controlled by the first electric telescopic rod 21 , and the sampling dish 23 can be driven to rotate by the electric turntable 22 .

[0038] A further optimized solution is that the driving assembly includes a screw 3, both ends of which are rotatably connected to fixed blocks 31, the fixed block 31 is fixedly connected to the top of the sampling chamber, the screw 3 passes through the fixed block 31 and is transmission-connected to a motor 32 at either end, the motor 32 is fixedly connected to the fixed block 31, the screw 3 is threadedly connected to a slider 33, one end of the slider 33 facing the diaphragm 2 is fixedly connected to a fixed end of a third electric telescopic rod 34, and the movable end of the third electric telescopic rod 34 is fixedly connected to the sampling assembly.

[0039] The motor 32 drives the lead screw 3 to rotate, the lead screw 3 drives the slider 33 to move, the slider 33 drives the third electric telescopic rod 34 to move, the third electric telescopic rod 34 drives the sampling assembly to move, and at the same time, the movable end of the third electric telescopic rod 34 can increase the moving range of the sampling assembly.

[0040] In order to provide stability in the movement of the slider 33 , the slider 33 shakes to form a limit groove, which is opened at the top of the sampling chamber. The slider 33 can move stably through the limit groove.

[0041] The control panel 14 is electrically connected to the motor 32 and the third electric telescopic rod 34 , and the operation of the motor 32 and the third electric telescopic rod 34 can be controlled by the control panel 14 .

[0042] A further optimized solution is that the sampling assembly includes a fixed end of a fourth electric telescopic rod 35 fixedly connected to the movable end of the third electric telescopic rod 34, and a fifth electric telescopic rod 36 and an extraction tube 37 are fixedly connected to the side wall of the movable end of the fourth electric telescopic rod 35, and the fifth electric telescopic rod 36 is arranged above the extraction tube 37.

[0043] The movable end of the third electric telescopic rod 34 drives the fixed end of the fourth electric telescopic rod 35 to move, and the fourth electric telescopic rod 35 drives the fifth electric telescopic rod 36 and the extraction tube 37 to move up and down.

[0044] The control panel 14 is electrically connected to the fourth electric telescopic rod 35 and the fifth electric telescopic rod 36 , and the fourth electric telescopic rod 35 and the fifth electric telescopic rod 36 are controlled to work through the control panel 14 .

[0045] To further optimize the solution, a piston is fixedly connected to the movable end of the fifth electric telescopic rod 36, the piston extends into the extraction cylinder 37 and is slidably connected to the extraction cylinder 37, and the extraction cylinder 37 is fixedly connected to the center of one end of the diaphragm 2 and is connected with a needle tube.

[0046] The movable end of the fifth electric telescopic rod 36 is extended and retracted, so that the movable end of the fifth electric telescopic rod 36 drives the piston to slide in the extraction cylinder 37, so that the needle tube can perform sampling and sample discharge work.

[0047] A further optimized solution is that a grinder 41 is fixedly connected in the processing chamber, the grinder 41 is arranged near the vertical partition 4, the top of the grinder 41 is fixedly connected and connected with a feed pipe, the end of the feed pipe away from the grinder 41 passes through the horizontal partition 2 and extends into the sampling chamber, the lower part of the outer wall of the grinder 41 is fixedly connected and connected with a discharge pipe, the discharge pipe is connected to a centrifuge 43 through a telescopic hose 42, and the centrifuge 43 is fixedly connected in the processing chamber near the discharge sealing door 13.

[0048] The pulverizer 41 has the functions of pulverizing and grinding.

[0049] Open the valve on the feed pipe to allow the sample to enter the grinder 41 through the feed pipe. The grinder 41 is used to grind and grind the sample. After the grinding is completed, open the valve on the discharge pipe to allow the sample to enter the centrifuge 43 through the telescopic hose 42 for centrifugal treatment.

[0050] The control panel 14 is electrically connected to the valve of the feed pipe, the valve of the discharge pipe, the pulverizer 41 and the centrifuge 43. The opening and closing of the valve of the feed pipe and the valve of the discharge pipe can be controlled by the control panel 14. The control panel 14 controls the operation of the pulverizer 41 and the centrifuge 43.

[0051] In a further optimized solution, the top surface of the centrifuge 43 is lower than the bottom surface of the pulverizer 41 , so that the pulverized and ground sample can flow into the centrifuge 43 effectively.

[0052] As a further optimization scheme, the top surface of the grinder 41 is connected with a liquid inlet pipe, one end of the liquid inlet pipe away from the grinder 41 passes through the observation window 11, and a silicone sealing plug is installed in the end of the liquid inlet pipe extending outside the observation window 11.

[0053] When the sampled sample enters the grinder 41, the cell lysate is extracted through the syringe, and after the syringe is inserted into the silicone sealing plug, the cell lysate flows into the grinder 41 through the liquid inlet tube, and then the grinder 41 performs a grinding operation.

[0054] Working process: before processing, the refrigerator 5 is turned on, and the refrigeration temperature of the refrigerator 5 is adapted to the refrigeration temperature of the sample. The temperature in the feed room, sampling room, equipment room and processing room is adapted to the refrigeration temperature of the sample through the refrigerator 5. The temperature conditions in the feed room, sampling room, equipment room and processing room are checked through the control panel 14. After the temperature meets the requirements, the sample is taken out of the refrigeration room, the feed sealing door 12 is opened, and the sample is placed in the sampling dish 23. At this time, the movable sealing plate 24 is in a closed state, that is, the feed room and the sampling room are in an isolated state. Then, after the feed sealing door 12 is closed, the ultraviolet emitter 6 and the electric turntable 22 are turned on, and the electric The turntable 22 drives the sampling dish 23 to rotate, and sterilizes the feeding chamber, sampling chamber, equipment chamber, and processing chamber through the ultraviolet emitter 6. After the sterilization is completed, the ultraviolet emitter 6 and the electric turntable 22 are turned off, and several second electric telescopic rods 25 act synchronously to lower the movable sealing plate 24. At this time, the feeding chamber and the sampling chamber are in a connected state. Then, the piston end of the first electric telescopic rod 21 extends to make the top surface of the sampling dish 23 higher than the top surface of the movable sealing plate 24. At this time, the motor 32 drives the lead screw 3 to rotate, the lead screw 3 drives the slider 33 to move, and the slider 33 drives the third electric telescopic rod 34 to move. When the slider 33 moves to the fixed block 31 near the feeding chamber, the electric The machine 32 is closed. At this time, the movable end of the third electric telescopic rod 34 extends out, and the movable end of the third electric telescopic rod 34 drives the fourth electric telescopic rod 35 to move. The fourth electric telescopic rod 35 drives the fifth electric telescopic rod 36 and the extraction tube 37 to move. When the needle tube on the extraction tube 37 is above the sample, the movable end of the fourth electric telescopic rod 35 extends out. At the same time, the movable end of the fifth electric telescopic rod 36 drives the piston to move toward the needle tube. When the needle tube is inserted into the sample, the movable end of the fifth electric telescopic rod 36 is retracted, and the sample enters the extraction tube 37 along the needle tube. After the extraction is completed, the movable end of the fourth electric telescopic rod 35 is retracted, and the motor 32 is reversed to make the extraction tube 37 at the feed of the pulverizer 41. The upper part of the tube is then extended by the movable end of the fourth electric telescopic rod 35, and the valve on the feed tube is opened synchronously. When the needle tube is inserted into the feed tube, the movable end of the fifth electric telescopic rod 36 is extended to discharge the sample in the extraction tube 37 into the feed tube. Then, the extraction tube 37 is reset, and then, the cell lysate is extracted through the syringe, and after the syringe is inserted into the silicone sealing plug, the cell lysate flows into the grinder 41 through the liquid inlet pipe, and then the grinder 41 performs the crushing and grinding operation. After the crushing and grinding is completed, the valve on the discharge pipe is opened to allow the sample to enter the centrifuge 43 through the telescopic hose 42 for centrifugal treatment. After the centrifugation is completed, the discharge sealing door 13 is opened for collection.

[0055] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0056] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A tissue processing device for protein purification, characterized in that: The invention comprises a box body (1), wherein the box body (1) is divided into an upper chamber and a lower chamber by a transverse partition (2), wherein the upper chamber is divided into a feed chamber and a sampling chamber for placing samples by a movable sealing plate (24), and the lower chamber is divided into an equipment chamber and a processing chamber for sampling by a vertical partition, wherein one end of the movable sealing plate (24) extending through the transverse partition into the equipment chamber is fixedly connected to the movable ends of a plurality of second electric telescopic rods (25), a refrigerator (5) for cooling the feed chamber, the sampling chamber, the equipment chamber, and the processing chamber is provided on one side of the box body (1), and ultraviolet emitters (6) for sterilization are respectively installed in the feed chamber, the sampling chamber, the equipment chamber, and the processing chamber; A driving assembly is arranged on the top of the sampling chamber, and a sampling assembly for sampling is fixedly connected to the driving assembly.

2. The tissue processing device for protein purification according to claim 1, characterized in that: An observation window (11) is embedded on the side of the box body (1) facing away from the refrigerator (5), a first through groove connected to the feed chamber is opened on one side of the box body (1), a feed sealing door (12) is installed in the first through groove, and a second through groove connected to the processing chamber is opened on the end of the box body (1) facing away from the first through groove, a discharge sealing door (13) is installed in the second through groove.

3. The tissue processing device for protein purification according to claim 2, characterized in that: A control panel (14) is arranged above the discharge sealing door (13), and the control panel (14) is embedded on the outer wall of the box body (1).

4. The tissue processing device for protein purification according to claim 1, characterized in that: A sampling dish (23) is arranged at the lower part of the feed chamber, the bottom surface of the sampling dish (23) is fixedly connected to the rotating end of an electric turntable (22), the fixed end of the electric turntable (22) is fixedly connected to the movable end of a first electric telescopic rod (21), the fixed end of the first electric telescopic rod (21) penetrates the transverse partition (2) and extends into the equipment chamber, and the fixed end of the first electric telescopic rod (21) is fixedly connected to the bottom of the equipment chamber.

5. The tissue processing device for protein purification according to claim 1, characterized in that: The driving assembly comprises a lead screw (3), both ends of the lead screw (3) are rotatably connected to fixed blocks (31), the fixed block (31) is fixedly connected to the top of the sampling chamber, the lead screw (3) passes through either end of the fixed block (31) and is transmission-connected to a motor (32), the motor (32) is fixedly connected to the fixed block (31), the lead screw (3) is threadedly connected to a slider (33), one end of the slider (33) facing the transverse partition (2) is fixedly connected to the fixed end of a third electric telescopic rod (34), and the movable end of the third electric telescopic rod (34) is fixedly connected to the sampling assembly.

6. The tissue processing device for protein purification according to claim 5, characterized in that: The sampling assembly comprises a fixed end of a fourth electric telescopic rod (35) fixedly connected to the movable end of the third electric telescopic rod (34); a fifth electric telescopic rod (36) and an extraction tube (37) are fixedly connected to the side wall of the movable end of the fourth electric telescopic rod (35); and the fifth electric telescopic rod (36) is arranged above the extraction tube (37).

7. The tissue processing device for protein purification according to claim 6, characterized in that: A piston is fixedly connected to the movable end of the fifth electric telescopic rod (36), and the piston extends into the extraction cylinder (37) and is slidably connected to the extraction cylinder (37). The extraction cylinder (37) is fixedly connected to the center of one end of the diaphragm (2) and is connected with a needle tube.

8. The tissue processing device for protein purification according to claim 2, characterized in that: A pulverizer (41) is fixedly connected in the processing chamber, and the pulverizer (41) is arranged close to the vertical partition (4). A feed pipe is fixedly connected to the top of the pulverizer (41) and is connected thereto. One end of the feed pipe away from the pulverizer (41) passes through the transverse partition (2) and extends into the sampling chamber. A discharge pipe is fixedly connected to the lower part of the outer wall of the pulverizer (41) and is connected thereto. The discharge pipe is connected to a centrifuge (43) via a telescopic hose (42). The centrifuge (43) is fixedly connected in the processing chamber close to the discharge sealing door (13).

9. The tissue processing device for protein purification according to claim 8, characterized in that: The top surface of the centrifuge (43) is lower than the bottom surface of the pulverizer (41).

10. The tissue processing device for protein purification according to claim 8, characterized in that: The top surface of the pulverizer (41) is connected to a liquid inlet pipe, one end of the liquid inlet pipe away from the pulverizer (41) passes through the observation window (11), and a silicone sealing plug is installed in the end of the liquid inlet pipe extending outside the observation window (11).

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