A detection and analysis device for stem cell exosome uric acid-lowering preparations

Abstract

The invention relates to the technical field of preparation detection and analysis, and discloses a detection and analysis device for a stem cell exosome uric acid-lowering preparation, comprising: a column oven and an automatic sampler arranged on the column oven, wherein two sample seats are placed in the automatic sampler, and multiple rows of seat holes are provided on the sample seats to place sample tubes, and further comprising: a sample transfer part, which is fixed in the automatic sampler, located between the two sample seats, and connected to the sample seats; an internal standard part, which is fixed between the automatic sampler and a detector, and extends into the automatic sampler; a label emitting part, which runs through the internal standard part and corresponds to the position of a row of seat holes; a linkage part, which is arranged on the label emitting part and is located in the label emitting part; the invention utilizes the internal standard part to store internal standards, and in the process of translating the sample tube into the automatic sampler, the linkage part and the label emitting part are triggered, and as the sample tube moves, the label emitting part is inserted into the sample tube at the corresponding position and the internal standard is added in batches during the moving process, thereby improving work efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of preparation detection and analysis, and in particular to a detection and analysis device for a stem cell exosome uric acid-lowering preparation. Background Art

[0002] In the technical field of preparation detection and analysis, it is usually necessary to use a liquid chromatograph composed of a column oven, an automatic sampler, a detector, an infusion pump and a bottle rack to detect and analyze the stem cell exosome uric acid-lowering preparation. The staff needs to put the stem cell exosome uric acid-lowering preparation in a sample tube, and arrange it on the sample seat using the seat holes, and use the sample seat to move the arranged sample tubes containing the preparation into the automatic sampler, and at the same time start the automatic sampler, the detector, the column oven and the infusion pump, so that the automatic sampler performs automatic sampling, so that the preparation in the sample tube can enter the detector, and at the same time cooperate with the column oven and the infusion pump to enable the detector to detect and analyze the preparation. However, the traditional detection and analysis device for stem cell exosome uric acid-lowering preparation still has some problems during the working process.

[0003] For example, in actual operation, when the accuracy of detection and analysis needs to be improved, the staff is required to add internal standards to the samples in the sample tubes one by one. When encountering batches of sample tubes, adding internal standards will consume a lot of time and the work efficiency is low. Summary of the invention

[0004] The present invention provides a detection and analysis device for a stem cell exosome uric acid-lowering preparation, which utilizes an internal standard part to store an internal standard, and in the process of using a sample holder to carry a sample tube and translate it into an automatic sampler, triggers a linkage part and a label-discharging part, and as the sample tube moves, the label-discharging part is inserted into the sample tube at a corresponding position and the internal standard is added in batches during the moving process, thereby improving work efficiency and reducing labor costs.

[0005] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0006] In the first aspect, a detection and analysis device for a stem cell exosome uric acid lowering preparation comprises: a column temperature box and an automatic sampler disposed on the column temperature box, the automatic sampler is provided with a detector, the detector is provided with an infusion pump to cooperate with the detection of the cell exosome uric acid lowering preparation, the infusion pump is fixed with a bottle rack, two sample seats are placed in the automatic sampler, and multiple rows of seat holes are provided on the sample seat to place sample tubes, the inner sides of the column temperature box, the automatic sampler, the detector and the infusion pump are rotatably connected with a box door, and further comprises:

[0007] A sample moving part is fixed in the automatic sampler and is located between the two sample seats, and is connected to the sample seats to automatically move the sample seats;

[0008] An internal standard part is fixed between the automatic sample injector and the detector and extends into the automatic sample injector to store an internal standard substance for improving the accuracy of detecting the exosome uric acid-lowering preparation;

[0009] The label discharge part is arranged through the internal label part and corresponds to the position of a row of seat holes to provide a channel for discharging the internal standard stored in the internal label part to the sample tube at the corresponding position;

[0010] The linkage part is arranged on the label discharging part and is located inside the label discharging part, so that when the sample holder is moved into the automatic sampler, the linkage part is pushed by the sample tube to control the label discharging part to be inserted into the sample tube to discharge the internal standard;

[0011] The marking part includes a drop-through piece and an inserting piece, wherein the drop-through piece is connected to the inner marking part and communicates with the inner marking part, and the inserting piece is movably connected through the drop-through piece and communicates with the drop-through piece;

[0012] The linkage part includes a pressure blocking member and a control member, the pressure blocking member is connected to the control member, the pressure blocking member is fixedly connected to the inner mark part, and is movably connected to the lowering member, the control member is arranged in the lowering member, penetrates the lowering member and is connected to the pressure blocking member.

[0013] Further, the sample transfer part includes:

[0014] An electric rod, one end of which is fixed to the inner wall of the automatic sampler;

[0015] A connecting plate, fixed to the other end of the electric pole;

[0016] A fixed rod has one end fixed on the connecting plate and the other end fixedly connected to the sample holder.

[0017] Further, the internal standard part includes:

[0018] A storage component, fixed on the automatic sampler and located between the automatic sampler and the detector, for storing the internal standard;

[0019] A flow guide member is arranged on the storage member and extends into the automatic sample injector;

[0020] The flow guide is connected to and communicates with the storage member to guide the flow of the internal standard stored in the storage member.

[0021] Furthermore, the downgraded parts include:

[0022] There are multiple connecting tubes, which are fixed on the inner side of the automatic sample injector and communicated with the flow guide, and their positions correspond to a row of hole seats near the box door;

[0023] A bellows, arranged at the end of the connecting pipe away from the storage member;

[0024] The row pipe is fixed to the end of the bellows away from the connecting pipe;

[0025] The flared tube is fixed to the end of the row pipe away from the corrugated tube.

[0026] Further, the inserting tube comprises:

[0027] The embedded guide groove is provided inside the flared pipe and penetrates the two sides of the flared pipe close to and away from the box door, and penetrates the end of the flared pipe away from the bellows;

[0028] The rubber plate is embedded in the inner side of the embedding guide groove and penetrates the embedding guide groove;

[0029] The pointed tube is opened through the inside of the rubber sheet.

[0030] Further, the pressure-blocking member comprises:

[0031] There are multiple support rods, one end of each of which is fixed below the flow guide and is staggered with the connecting pipes;

[0032] A rotating support cover is fixed to the other end of the support rod and is located on one side of the flared pipe;

[0033] A rotary plate is rotatably connected to the inner side of the rotary support cover;

[0034] Auxiliary parts, fixed on the rotating plate;

[0035] The guide component is arranged on the outside of the row pipe and is connected with the auxiliary component for guiding.

[0036] Further, the auxiliary parts include:

[0037] A stopper is fixed to one end of the rotary plate close to the sample holder and is located at a position of the rotary plate away from the chamber door;

[0038] The arc head plate is rotatably connected to the inner side of the rotating plate, penetrates the rotating plate, and fits with the side of the stopper close to the box door;

[0039] The L rod is fixed on the outer side of the rotating plate and passes through the rotating support cover.

[0040] Further, the guide component comprises:

[0041] Pull plate, fixed on the outside of the row pipe;

[0042] A movable groove is provided through the interior of the pull plate;

[0043] A guide groove is provided through the pull plate and is connected with the moving groove;

[0044] The guide rod passes through the L rod and is fixedly connected thereto, and is located inside the guide groove and the movable groove.

[0045] Furthermore, the control member includes:

[0046] The rack rods are symmetrically fixed on the inner side of the connecting pipe;

[0047] The support plate is arranged on the inner side of the connecting pipe and is fixedly connected to the frame rod;

[0048] The reed is symmetrically arranged on a side of the support plate close to the sample holder;

[0049] The rubber plug is fixed to the end of the reed away from the supporting plate and is located below the connecting tube.

[0050] Furthermore, the control member includes:

[0051] C rod, fixed at both ends of the guide rod and located outside the pull plate;

[0052] A steel wire rope, one end of which is fixed to the side of the rubber plug away from the reed, and the other end of which passes through the bellows;

[0053] The collar is fixed to the other end of the wire rope and is mounted on the outside of the C rod;

[0054] The sealing ring penetrates the interior of the corrugated tube and is fixedly connected thereto, and is sleeved on the outside of the steel wire rope.

[0055] The above solution of the present invention includes at least the following beneficial effects:

[0056] By providing a label arrangement part and a linkage part, before adding an internal standard substance into the sample tube, an appropriate amount of internal standard substance needs to be injected into the internal standard part for storage, and then the sample seat is removed from the automatic sampler, and the sample tube containing the sample is placed in the hole seat on the sample seat for arrangement, and the sample tube carried by the sample seat is translated into the automatic sampler. During the translation process, the sample tube will contact with the blocking and pressure parts provided in the linkage part as it moves, and the blocking and pressure parts push the control part, so that the control part opens the channel of the lowering part, so that the internal standard substance stored in the internal standard part can enter the lowering part, and at the same time, the control part will pull the lowering part downward, so that the lowering part drives the inserting part to be inserted into the sample tube, and at the same time, when the sample tube moves, it will drive the inserting part to translate for a distance together, so that the internal standard substance stored in the internal standard part can pass through the lowering part and the inserting part and be injected into the sample tube in batches, thereby improving work efficiency and reducing labor costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 A schematic diagram of the overall three-dimensional structure of a detection and analysis device for a stem cell exosome uric acid-lowering preparation provided in an embodiment of the present invention;

[0058] Figure 2 A schematic diagram of the three-dimensional structure of the automatic sample injector, sample holder and frame cover combination provided by an embodiment of the present invention;

[0059] Figure 3 A schematic diagram of the three-dimensional structure of the automatic sample injector and storage capsule combination provided in an embodiment of the present invention;

[0060] Figure 4 A schematic diagram of the three-dimensional structure of the combination of the inner marking part, the marking arrangement part and the linkage part provided in an embodiment of the present invention;

[0061] Figure 5 A schematic diagram of the three-dimensional structure of a sample holder and a motor assembly provided in an embodiment of the present invention;

[0062] Figure 6 The embodiment of the present invention provides Figure 5 Schematic diagram of the structure at A in FIG.

[0063] Figure 7 A schematic diagram of the three-dimensional structure of the combination of the label placement unit and the linkage unit provided in an embodiment of the present invention;

[0064] Figure 8 A three-dimensional exploded view of a flared tube, a bellows and a spring assembly provided in an embodiment of the present invention;

[0065] Fig. 9 A schematic diagram of a cross section of a flared tube and a three-dimensional structure of a combination of a rubber sheet and a pointed tube provided in an embodiment of the present invention;

[0066] Fig.10 A schematic diagram of a three-dimensional structure of a cross section of a flared tube and a combination of embedded guide grooves provided in an embodiment of the present invention;

[0067] Fig.11 A three-dimensional exploded view of the arc head plate, the rotary plate and the rotary support cover assembly provided in an embodiment of the present invention;

[0068] Fig.12 A schematic diagram of a three-dimensional structure of a pointed tube and a rubber sheet combination provided in an embodiment of the present invention;

[0069] Fig.13 The embodiment of the present invention provides Figure 7 Schematic diagram of the structure at B in FIG.

[0070] Fig.14 A schematic diagram of the three-dimensional position of the arc head plate, the pointed tube and the sample tube according to the embodiment of the present invention;

[0071] Fig.15 A schematic diagram of the cross section of the expanded tube and the connected tube and the three-dimensional structure of the combination of the support plate and the rubber plug provided in the embodiment of the present invention.

[0072] Description of reference numerals:

[0073] In the figure: 1, column temperature box; 2, automatic sampler; 3, detector; 4, infusion pump; 5, bottle rack; 6, box door; 7, sample holder; 8, sample tube; 9, electric rod; 10, connecting plate; 11, fixing rod; 12, storage capsule; 13, transparent plate; 14, cover; 15, frame cover; 16, inclined tube; 17, hollow plate; 18, fixing plate; 19, seat hole; 20, connecting tube; 21, expansion tube; 22, bellows; 23, row tube; 24, support ring; 25, spring; 26 , flared tube; 27, embedded guide groove; 28, rubber sheet; 29, pointed tube; 30, support rod; 31, rotary support cover; 32, rotary column; 33, rotary plate; 34, movable groove; 35, block; 36, rotating rod; 37, arc head plate; 38, L rod; 39, pull plate; 40, movable groove; 41, guide groove; 42, guide rod; 43, C rod; 44, rack rod; 45, support plate; 46, spring leaf; 47, rubber plug; 48, wire rope; 49, collar; 50, sealing ring. DETAILED DESCRIPTION

[0074] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present invention and to enable the scope of the present invention to be fully communicated to those skilled in the art.

[0075] like Figures 1 to 15 As shown, an embodiment of the present invention provides a detection and analysis device for a stem cell exosome uric acid lowering preparation, comprising: a column temperature box 1 and an automatic sampler 2 arranged on the column temperature box 1, a detector 3 is arranged on the automatic sampler 2, and an infusion pump 4 is arranged on the detector 3 to cooperate with the detection of the cell exosome uric acid lowering preparation, a bottle rack 5 is fixed on the infusion pump 4, two sample seats 7 are placed in the automatic sampler 2, and multiple rows of seat holes 19 are opened on the sample seat 7 to place sample tubes 8, and the inner sides of the column temperature box 1, the automatic sampler 2, the detector 3 and the infusion pump 4 are rotatably connected with a box door 6, characterized in that it also includes:

[0076] The sample moving part is fixed in the automatic sampler 2 and is located between the two sample holders 7, and is connected to the sample holders 7 to automatically translate the sample holders 7;

[0077] The internal standard part is fixed between the automatic sample injector 2 and the detector 3, and extends into the automatic sample injector 2 to store the internal standard substance for improving the accuracy of detecting the extracellular uric acid-lowering preparation;

[0078] The label discharge part is arranged through the internal label part and corresponds to the position of a row of seat holes 19 to provide a channel for discharging the internal standard stored in the internal label part to the sample tube 8 at the corresponding position;

[0079] The linkage part is arranged on the label discharging part and is located inside the label discharging part, so that when the sample holder 7 is moved into the automatic sampler 2, the sample tube 8 pushes the label discharging part to control the insertion of the label discharging part into the sample tube 8 to discharge the internal standard;

[0080] The mark-discharging part includes a drop-through piece and a pipe inserting piece, the drop-through piece is connected to the inner mark part and communicates with the inner mark part, and the pipe inserting piece is movably connected through the drop-through piece and communicates with the drop-through piece;

[0081] The linkage part includes a pressure blocking member and a control member, the pressure blocking member is connected to the control member, the pressure blocking member is fixedly connected to the inner mark part, and is movably connected to the lowering member, the control member is arranged in the lowering member, and penetrates the lowering member to connect with the pressure blocking member.

[0082] Specifically, the column temperature box 1 can provide stable support for the automatic sampler 2, the automatic sampler 2 can provide a placement space for the sample holder 7 and can automatically inject the sample in the sample tube 8 on the sample holder 7, and can provide support for the detector 3, so that the detector 3 can stably support the infusion pump 4, the infusion pump 4 can provide support for the bottle rack 5, and the bottle rack 5 can provide a placement space and support positioning for the mobile phase container.

[0083] In the actual application of this embodiment: the staff can put the prepared sample into the sample tube 8, and then position the sample tube 8 into the seat hole 19 on the sample seat 7, and then start the automatic sampler 2, the column oven 1, the detector 3 and the infusion pump 4 in the working order, and at the same time put the mobile phase and other solvents into the bottle rack 5 and connect them to the infusion pump 4, and then rinse with the mobile phase until the baseline is stable, and then set the injection sequence and data acquisition parameters, start the automatic sampler 2, start the analysis sequence, the sample is injected and passes through the chromatographic column, the detector 3 monitors the effluent in real time, and uses the software to record and process the original data to generate a chromatogram and report for the detection and analysis.

[0084] As a preferred embodiment of the present invention, the sample transfer part includes:

[0085] An electric rod 9, one end of which is fixed to the inner wall of the automatic sample injector 2;

[0086] A connecting plate 10 is fixed to the other end of the electric rod 9;

[0087] One end of the fixing rod 11 is fixed on the connecting plate 10 , and the other end is fixedly connected to the sample holder 7 .

[0088] Specifically, the automatic sample injector 2 can provide stable support for the electric rod 9, so that the electric rod 9 can stably support the connecting plate 10. The connecting plate 10 can be connected to the sample holder 7 through a fixing component. The fixing rod 11 can make the connecting plate 10 more stable and cooperate with the connecting plate 10 to provide support for the sample holder 7.

[0089] The internal standard department includes:

[0090] A storage component, fixed on the automatic sampler 2 and located between the automatic sampler 2 and the detector 3, for storing the internal standard;

[0091] A flow guide member, which is arranged on the storage member and extends into the automatic sample injector 2;

[0092] The flow guide is connected to and communicates with the storage member to guide the flow of the internal standard stored in the storage member.

[0093] Specifically, the storage device includes:

[0094] The storage capsule 12 is fixed on the automatic sample injector 2 and is located between the detector 3 and the automatic sample injector 2;

[0095] A transparent plate 13 is fixedly arranged through a side of the storage capsule 12 away from the automatic sample injector 2;

[0096] The cover 14 is sleeved on the storage bag 12 .

[0097] Specifically, the guide member comprises:

[0098] The frame cover 15 is fixed to the outside of the storage capsule 12 and is located on a side of the automatic sample injector 2 close to the box door 6 and above the box door 6;

[0099] The oblique tube 16 is symmetrically arranged through a side of the storage capsule 12 close to the automatic sample injector 2, and obliquely extends into the automatic sample injector 2, and is located inside the frame cover 15;

[0100] The hollow plate 17 is fixed to the end of the inclined tube 16 away from the storage capsule 12 and is located inside the automatic sample injector 2;

[0101] One end of the fixing plate 18 is fixed on the hollow plate 17 , and the other end is fixedly connected to the storage bag 12 .

[0102] In actual application of this embodiment, the staff can open the box door 6 with the support of the automatic sampler 2, and then control the electric rod 9 to extend, so that the electric rod 9 uses the telescopic end to push the connecting plate 10, and drives the fixing rod 11 and the sample holder 7 to move horizontally to the outside of the automatic sampler 2 through the connecting plate 10 and the fixing component, so that the sample holder 7 is moved out of the automatic sampler 2 to the outside. Then, the staff can place the sample tube 8 containing the sample into the seat hole 19 with the support of the sample holder 7, so that the sample holder 7 provides support for the sample tube 8. After the sample tube 8 is placed, the electric rod 9 is controlled to be retracted, so that the electric rod 9 uses the telescopic end to pull the sample holder 7 and the sample tube 8 through the connecting plate 10 to slowly move horizontally to the inside of the automatic sampler 2.

[0103] When it is necessary to add an internal standard substance into the sample tube 8, the staff needs to pull up the cover 14, remove the cover 14 to open the storage capsule 12, and then inject the internal standard substance into the inner side of the storage capsule 12, while observing the dosage of the internal standard substance in the storage capsule 12 through the transparent plate 13 at the head. After the storage capsule 12 is full, the cover 14 can be closed. At the same time, the internal standard substance in the storage capsule 12 will enter the inner side of the inclined tube 16 under the action of gravity, so that the inclined tube 16 provides a channel for the internal standard substance to enter the hollow plate 17 under the protection of the frame cover 15, so that the hollow plate 17, the inclined tube 16 and the storage capsule 12 cooperate to store the internal standard substance.

[0104] As a preferred embodiment of the present invention, the drop-through member comprises:

[0105] The connecting tube 20 has a plurality of connecting tubes 20, which are fixed to the inner side of the automatic sample injector 2 and communicated with the flow guide, and the positions thereof correspond to a row of holes near the box door 6;

[0106] The bellows 22 is arranged at one end of the connecting tube 20 away from the storage member;

[0107] The row pipe 23 is fixed to the end of the corrugated pipe 22 away from the connecting pipe 20;

[0108] The flared tube 26 is fixed to one end of the row tube 23 away from the corrugated tube 22 .

[0109] Specifically, the end of the connecting tube 20 away from the hollow plate 17 is fixed with an expansion tube 21, and the end of the expansion tube 21 away from the connecting tube 20 is fixedly connected to the bellows 22. The outer side of the row tube 23 is fixedly sleeved with a support ring 24. The outer side of the bellows 22 is sleeved with a spring 25, and one end of the spring 25 is fixedly connected to the support ring 24, and the other end is fixedly connected to the hollow plate 17. The hollow plate 17 can provide a stable support for the connecting tube 20, so that the connecting tube 20 can support the bellows 22, and the bellows 22 can expand and contract under the action of external force. The tube 22 can provide support for the row tube 23, the row tube 23 can stably support the expanded tube 26, the expanded tube 26 can provide a space for the embedded guide groove 27, the connecting tube 20 can stably support the expanded tube 21, so that the expanded tube 21 can provide support for the corrugated tube 22, the row tube 23 can provide support for the support ring 24, so that the support ring 24 can stably support the spring 25, and the hollow plate 17 can provide support for the end of the spring 25 away from the support ring 24, the spring 25 can be stretched and stored under the action of external force, and will rebound after the external force disappears.

[0110] The cannula includes:

[0111] The embedding guide groove 27 is provided inside the flared tube 26 and penetrates the two sides of the flared tube 26 close to and away from the door 6, and penetrates the end of the flared tube 26 away from the bellows 22;

[0112] The rubber plate 28 is embedded in the inner side of the embedding guide groove 27 and penetrates the embedding guide groove 27;

[0113] The pointed tube 29 is opened through the inside of the rubber plate 28 .

[0114] Specifically, the guide groove 27 can provide a moving guide for the rubber sheet 28 , and the rubber sheet 28 is made of rubber and is elastic. It can use elastic force to fit with the flared tube 26 through the guide groove 27 to achieve a sealing effect, and the rubber sheet 28 can provide a stable support for the pointed tube 29 .

[0115] In actual application of this embodiment, the internal standard substance in the hollow plate 17 will enter the inner side of the connecting tube 20 under the action of gravity. When the rubber stopper 47 is separated from the connecting tube 20, the opening of the connecting tube 20 will be opened, so that the internal standard substance entering the connecting tube 20 can enter the inner side of the bellows 22, and enter the inner side of the flared tube 26 along the bellows 22, and finally pass through the flared tube 26 into the pointed tube 29, and along the pointed tube 29, after the pointed tube 29 is inserted into the sample tube 8, the internal standard substance will be injected into the sample in the sample tube 8. After the pointed tube 29 is inserted into the sample tube 8, when the sample tube 8 moves horizontally, the rubber plate 28 will be pushed by the pointed tube 29 to move along the embedded guide groove 27 under the support of the flared tube 26.

[0116] In another preferred embodiment of the present invention, the pressure-blocking member comprises:

[0117] There are multiple support rods 30, one end of each of which is fixed below the guide member and is staggered with the connecting pipe 20;

[0118] The rotating support cover 31 is fixed to the other end of the support rod 30 and is located on one side of the expanded tube 26;

[0119] The rotary plate 33 is rotatably connected to the inner side of the rotary support cover 31;

[0120] Auxiliary parts, fixed on the rotating plate 33;

[0121] The guide component is arranged outside the row tube 23 and is connected to the auxiliary component for guiding.

[0122] Specifically, one end of the support rod 30 away from the rotary support cover 31 is fixedly connected to the hollow plate 17, and the internal rotation of the rotary support cover 31 is connected with a rotary column 32, and the rotary column 32 is connected to the rotary plate 33, and a movable groove 34 is opened inside the rotary plate 33. The hollow plate 17 can provide a stable support for the support rod 30, so that the support rod 30 can stably support the rotary support cover 31, and the rotary support cover 31 can provide a rotating support for the rotary column 32, the rotary column 32 can provide support for the rotary plate 33, and the rotary support cover 31 can provide a rotating space for the rotary plate 33. The rotary plate 33 can drive the rotary column 32 to rotate counterclockwise under the support of the rotary support cover 31 under the action of external force, and can provide space for the movable groove 34.

[0123] Auxiliary parts include:

[0124] The stopper 35 is fixed to one end of the rotary plate 33 close to the sample holder 7 and is located at a position where the rotary plate 33 is far away from the chamber door 6;

[0125] The arc head plate 37 is rotatably connected to the inner side of the rotating plate 33, penetrates the rotating plate 33, and fits with a side of the stopper 35 close to the box door 6;

[0126] The L rod 38 is fixed on the outer side of the rotary plate 33 and passes through the rotary support cover 31 .

[0127] Specifically, the internal rotation of the rotary plate 33 is connected with a rotating rod 36, the arc head plate 37 is located on the inner side of the movable groove 34, and is fixedly connected to the rotating rod 36, the rotary plate 33 can firmly support the block 35, and the block 35 can provide a resistance for the arc head plate 37 to prevent the arc head plate 37 from rotating clockwise from the block 35, the rotary plate 33 can provide a firm support for the L rod 38, and can drive the L rod 38 to rotate together under the action of external force, and the arc head plate 37 can use external force to push the rotary plate 33 to rotate clockwise through the block 35.

[0128] Pull guide parts include:

[0129] A pull plate 39 is fixed on the outside of the row tube 23;

[0130] The movable groove 40 is opened through the interior of the pull plate 39;

[0131] A guide groove 41 is formed through the pull plate 39 and communicates with the moving groove 40;

[0132] The guide rod 42 passes through the L rod 38 and is fixedly connected thereto, and is located inside the guide groove 41 and the moving groove 40 .

[0133] Specifically, the row pipe 23 can provide stable support for the support ring 24, the support ring 24 can provide stable support for the pull plate 39, the pull plate 39 can provide opening space for the movable groove 40 and the guide groove 41, the guide groove 41 can provide a movable guiding function for the guide rod 42, and the movable groove 40 can provide a moving space for the L rod 38.

[0134] Control components include:

[0135] The support rod 44 is symmetrically fixed on the inner side of the connecting pipe 20;

[0136] The support plate 45 is arranged on the inner side of the connecting tube 20 and is fixedly connected to the frame rod 44;

[0137] The spring 46 is symmetrically arranged on a side of the support plate 45 close to the sample holder 7;

[0138] The rubber plug 47 is fixed to one end of the spring 46 away from the support plate 45 and is located below the connecting tube 20 .

[0139] Specifically, the rubber plug 47 is located on the inner side of the expansion tube 21 and fits with the inner wall of one end of the expansion tube 21 close to the connecting tube 20. The connecting tube 20 can stably support the frame rod 44, so that the frame rod 44 stably supports the support plate 45. The support plate 45 can provide stable support for the spring leaf 46. The spring leaf 46 has a certain elasticity and can be deformed under the action of external force. It will rebound after the external force disappears and can provide support for the rubber plug 47. The rubber plug 47 is made of rubber material and has a certain elasticity. It can fit with the expansion tube 21 to play a sealing role.

[0140] Control components include:

[0141] C rod 43, fixed at both ends of the guide rod 42 and located outside the pull plate 39;

[0142] A steel wire rope 48, one end of which is fixed to the side of the rubber plug 47 away from the reed 46, and the other end of which passes through the bellows 22;

[0143] A collar 49 is fixed to the other end of the steel wire rope 48 and is sleeved on the outside of the C rod 43;

[0144] The sealing ring 50 penetrates the interior of the corrugated tube 22 and is fixedly connected thereto, and is sleeved on the outside of the steel wire rope 48 .

[0145] Specifically, the guide rod 42 can provide stable support for the C rod 43, the C rod 43 can provide a mounting space for the sleeve 49, the wire rope 48 can connect the rubber plug 47 with the sleeve 49, and the wire rope 48 can bend under the action of external force, and the bellows 22 can provide a through channel and stable support for the sealing ring 50. The sealing ring 50 is made of rubber and can fit with the wire rope 48 to play a sealing role.

[0146] In actual application of this embodiment, when the electric rod 9 contracts and pulls the sample tube 8 through the sample holder 7 to slowly translate into the automatic sampler 2, the sample tube 8 will contact the arc head plate 37 during the movement, and use the translational external force to push the arc head plate 37 under the support of the sample holder 7, so that the arc head plate 37 uses the external force to push the stopper 35, so that the stopper 35 pushes the rotary plate 33 to drive the rotary column 32 to rotate clockwise under the support of the rotary support cover 31. At the same time, the rotary plate 33 will drive the L rod 38 to rotate clockwise under the action of the external force, so that the L rod 38 uses the rotational power to push the pull plate 39 downward through the guide rod 42, and at the same time, it will drive the guide rod 42 and the L rod 38 to move along the moving plate 39. The movable groove 40 and the guide groove 41 move toward the direction approaching the box door 6, so that the pull plate 39 stretches the spring 25 downward through the support ring 24 under the action of external force, and at the same time, the support ring 24 will stretch the bellows 22 downward through the row tube 23, so that the bellows 22 and the spring 25 are stretched and extended under the support of the hollow plate 17, and at the same time, the spring 25 will store energy, and then the row tube 23 will drive the flared tube 26, the rubber plate 28 and the pointed tube 29 to move downward together under the action of external force. At the same time, with the translation of the sample tube 8, the rotated arc head plate 37 will move above the sample tube 8. At this time, the pointed tube 29 will move downward and use the external force and its own sharp end to insert into the inner side of the sample tube 8.

[0147] When the L rod 38 rotates clockwise with the rotating plate 33, the C rod 43 is driven to move together through the guide rod 42, so that the C rod 43 uses external force to pull the wire rope 48 through the ring 49 during the movement, so that the wire rope 48 passes through the sealing ring 50 under the action of the external force and moves away from the support plate 45. Then, the wire rope 48 can use external force to pull the rubber plug 47 downward under the support of the support plate 45 and the frame rod 44 during the movement, so that the rubber plug 47 stretches the spring 46 to store energy, and then the rubber plug 47 is separated from the expansion tube 21, thereby opening the passage between the expansion tube 21 and the connecting tube 20, so that the internal standard in the hollow plate 17 can pass through the connecting tube 20 into the inner side of the expansion tube 21, and pass through the expansion tube 21, the bellows 22, the row tube 23, the flared tube 26 and the pointed tube 29 under the action of gravity to be injected into the sample tube 8.

[0148] As the sample tube 8 translates, the arc plate 37 moved onto the sample tube 8 will separate from the sample tube 8, and then after the separation, the arc plate 37 and the rotary plate 33 will drive the rotary column 32 to rotate counterclockwise under the action of gravity and the support of the rotary support cover 31, and the rotary plate 33 will drive the L rod 38 to rotate counterclockwise, and at the same time, the tension on the wire rope 48 and the pull plate 39 will be released, so that the spring 25 uses the rebound force to pull the support ring 24 upward under the support of the hollow plate 17, so that the support ring 24 drives the pull plate 39, the row tube 23, the flared tube 26, the rubber plate 28 and the pointed tube 29 to move upward and reset, so that the pointed tube 29 is removed from the sample tube 8. The bellows 22 is pulled out from the tube 8, and the support ring 24 uses the rebound force of the spring 25 to squeeze the bellows 22 upward, so that the stretched bellows 22 can be folded and retracted to reset. At the same time, the L rod 38 drives the guide rod 42 to move and reset along the guide groove 41 in the direction away from the box door 6, and then the guide rod 42 drives the ring 49 to move together through the C rod 43, and then contacts the pulling force on the wire rope 48, so that the spring leaf 46 uses the rebound force to pull the rubber plug 47 upward under the support of the support plate 45, so that the rubber plug 47 pulls the wire rope 48 upward to reset, and at the same time, the rubber plug 47 fits with the inner wall of the expansion tube 21 to seal the passage between the expansion tube 21 and the connecting tube 20.

[0149] When the detection and analysis work is completed, the staff needs to open the box door 6 and control the electric rod 9 to extend, so that the electric rod 9 pushes the sample holder 7 and the sample tube 8 toward the box door 6 through the connecting plate 10, and translates toward the outside of the automatic sampler 2. In the process of the sample tube 8 translating toward the box door 6, it will contact the side of the arc head plate 37 away from the box door 6, so that the sample tube 8 uses external force to push the arc head plate 37, which drives the rotating rod 36 to rotate counterclockwise on the inner side of the movable groove 34 with the rotating rod 36 as the center under the support of the rotating plate 33, so that the arc head plate 37 can move to the sample tube 8 after rotation, so that the arc head plate 37 will not affect the removal of the sample tube 8.

[0150] Working principle: the staff puts the prepared sample into the sample tube 8 and places the sample tube 8 in the seat hole 19 on the sample seat 7. During the detection process, the automatic sampler 2 injects the sample in the sample tube 8 into the chromatographic column. The detector 3 monitors the effluent in real time, and records and processes the raw data through software to generate chromatograms and reports.

[0151] The internal standard part is used to store the internal standard, and in the process of using the sample holder 7 to carry the sample tube 8 and translate it into the automatic sampler 2, the linkage part and the label-discharging part are triggered. As the sample tube 8 moves, the label-discharging part is inserted into the sample tube 8 at the corresponding position and the internal standard is added in batches during the moving process.

[0152] When it is necessary to add an internal standard substance into the sample tube 8, the sample tube 8 will push the arc plate 37 during translation, so that the arc plate 37 drives the block 35 and the rotating plate 33 to rotate clockwise, and then pulls the wire rope 48 through the L rod 38, the guide rod 42 and the pull plate 39, so that the rubber plug 47 is separated from the expansion tube 21, and the passage of the connecting tube 20 is opened. The internal standard substance is injected into the sample tube 8 through the connecting tube 20, the expansion tube 21, the bellows 22, the row tube 23, the flared tube 26 and the pointed tube 29.

[0153] After the test is completed, the sample tube 8 is translated to the outside of the automatic sampler 2 by the electric rod 9. During the translation of the sample tube 8, the arc head plate 37 is separated from the sample tube 8, the rotary plate 33 and the L rod 38 are reset counterclockwise, the wire rope 48 and the rubber plug 47 are reset, and the passage between the expansion tube 21 and the connecting tube 20 is sealed. After the sample tube 8 is removed, the device can be cleaned and prepared for the next test.

[0154] Through the coordinated work of the above-mentioned components, the present invention can realize efficient and accurate detection and analysis of stem cell exosome uric acid-lowering preparations, thereby improving the automation and accuracy of detection.

[0155] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A detection and analysis device for a stem cell exosome uric acid-lowering preparation, comprising: A column temperature box (1) and an automatic sampler (2) arranged on the column temperature box (1), wherein the automatic sampler (2) is provided with a detector (3), and the detector (3) is provided with an infusion pump (4) to cooperate with the detection of an extracellular uric acid-lowering preparation, and a bottle rack (5) is fixed on the infusion pump (4), and two sample seats (7) are placed in the automatic sampler (2), and a plurality of rows of seat holes (19) are provided on the sample seat (7) to place sample tubes (8), and the inner sides of the column temperature box (1), the automatic sampler (2), the detector (3) and the infusion pump (4) are rotatably connected to a box door (6), characterized in that it also includes: A sample moving part, fixed in the automatic sample injector (2), located between the two sample holders (7), and connected to the sample holder (7) to automatically translate the sample holder (7); An internal standard portion is fixed between the automatic sample injector (2) and the detector (3) and extends into the automatic sample injector (2) to store an internal standard substance for improving the accuracy of detecting the extracellular uric acid-lowering preparation; A label discharge portion, which is arranged through the internal label portion and corresponds to the position of a row of seat holes (19) to provide a channel for discharging the internal standard substance stored in the internal label portion to the sample tube (8) at the corresponding position; The linkage part is arranged on the label discharging part and is located inside the label discharging part, so that when the sample holder (7) is moved into the automatic sample injector (2), the linkage part is pushed by the sample tube (8) to control the label discharging part to be inserted into the sample tube (8) to discharge the internal standard; The marking part includes a drop-through piece and an inserting piece, wherein the drop-through piece is connected to the inner marking part and communicates with the inner marking part, and the inserting piece is movably connected through the drop-through piece and communicates with the drop-through piece; The linkage part includes a pressure-blocking member and a control member, the pressure-blocking member is connected to the control member, the pressure-blocking member is fixedly connected to the inner portion, and is movably connected to the lowering member, the control member is arranged in the lowering member, penetrates the lowering member and is connected to the pressure-blocking member; The sample transfer parts include: An electric rod (9), one end of which is fixed to the inner wall of the automatic sample injector (2); A connecting plate (10) fixed to the other end of the electric rod (9); A fixing rod (11), one end of which is fixed to the connecting plate (10), and the other end of which is fixedly connected to the sample holder (7); The internal standard part comprises: A storage component, fixed on the automatic sample injector (2) and located between the automatic sample injector (2) and the detector (3), for storing an internal standard; A flow guide member, arranged on the storage member and extending into the automatic sample injector (2); The flow guide is connected to and communicates with the storage member to guide the flow of the internal standard stored in the storage member; The drop-in parts include: A plurality of connecting tubes (20) are fixed to the inner side of the automatic sample injector (2) and are connected to the flow guide member, and their positions correspond to a row of hole seats near the chamber door (6); A bellows (22) is arranged at an end of the connecting tube (20) away from the storage member; A row pipe (23) is fixed to an end of the corrugated pipe (22) away from the connecting pipe (20); A flared tube (26) fixed to an end of the row tube (23) away from the corrugated tube (22); The cannula comprises: An embedding guide groove (27) is provided inside the flared tube (26), penetrates two sides of the flared tube (26) close to and away from the box door (6), and penetrates one end of the flared tube (26) away from the bellows (22); A rubber plate (28) is embedded in the inner side of the embedding guide groove (27) and penetrates the embedding guide groove (27); A pointed tube (29) is provided inside the rubber sheet (28); The pressure-blocking member comprises: There are a plurality of support rods (30), one end of each of the plurality of support rods (30) is fixed below the flow guide member and is arranged in a staggered manner with the connecting pipe (20); A rotating support cover (31) is fixed to the other end of the support rod (30) and is located on one side of the flared tube (26); A rotary plate (33) is rotatably connected to the inner side of the rotary support cover (31); Auxiliary parts, fixed on the rotating plate (33); A guide component is arranged outside the row pipe (23) and is connected to the auxiliary component in a guiding manner; The auxiliary parts include: A stopper (35) is fixed to one end of the rotary plate (33) close to the sample holder (7) and is located at a position of the rotary plate (33) away from the chamber door (6); The arc head plate (37) is rotatably connected to the inner side of the rotating plate (33), penetrates the rotating plate (33), and is in contact with a side of the stopper (35) close to the box door (6); An L rod (38) is fixed to the outside of the rotary plate (33) and passes through the rotary support cover (31); The guide part comprises: A pull plate (39) fixed on the outside of the row pipe (23); A movable groove (40) is formed through the interior of the pull plate (39); A guide groove (41) is formed through the pull plate (39) and is communicated with the moving groove (40); A guide rod (42) passes through the L rod (38) and is fixedly connected thereto, and is located inside the guide groove (41) and the movable groove (40); The control member comprises: A support rod (44) is symmetrically fixed on the inner side of the connecting tube (20); A support plate (45) is arranged on the inner side of the connecting tube (20) and is fixedly connected to the frame rod (44); A reed (46) is symmetrically arranged on a side of the support plate (45) close to the sample holder (7); A rubber plug (47) is fixed to an end of the reed (46) away from the support plate (45) and is located below the connecting tube (20); A C rod (43) is fixed to both ends of the guide rod (42) and is located outside the pull plate (39); A steel wire rope (48), one end of which is fixed to a side of the rubber plug (47) away from the reed (46), and the other end of which passes through the bellows (22); A collar (49) is fixed to the other end of the steel wire rope (48) and is sleeved on the outside of the C rod (43); The sealing ring (50) penetrates the interior of the corrugated tube (22) and is fixedly connected thereto, and is sleeved on the outside of the steel wire rope (48).

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