Organic edible mushroom culture medium

Abstract

The invention discloses an organic edible mushroom culture medium, and relates to the technical field of culture media. 100 g of potatoes; 12 g of brown sugar; 11 g of glucose; 35 g of wheat bran; 1.8 g of white peptone; 1.8 g of monopotassium phosphate; 0.8 g of magnesium sulfate; a vitamin B11 tablet; 0.35 ml of polyoxypropylene glycerol; 1.0 g of gibberellin; and 0.75 g of calcium superphosphate. When the organic edible mushroom culture medium is used, the culture medium is prevented from being polluted by microorganisms in the environment through multi-batch sterilization operation, whether the quality of the culture medium meets the standard or not is judged through comparison test and performance test, then whether the culture medium is polluted by infectious microbes or not is found in time, and then the influence of the infectious microbes on the culture medium is eliminated; the quality of the culture medium is prevented from being influenced by reproduction of microorganisms in the culture medium, and a more suitable growth environment is provided for edible mushrooms, so that the culture period of the organic edible mushroom culture medium is shortened, and the culture efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of culture medium, in particular to an organic edible fungus culture medium. Background Art

[0002] Organic edible fungi refer to edible fungi that are produced without the use of chemically synthesized pesticides, fertilizers, preservatives, artificial synthetic pigments, herbicides, growth regulators and other substances during the production process; and organic edible fungi culture medium is a mixture of nutrients specially designed for the cultivation of edible fungi. Its main function is to provide the nutrients and environmental conditions required for the growth and reproduction of edible fungi, promote the growth and reproduction of mycelium, nutritional growth and the formation of fruiting bodies, etc.

[0003] In the production process of organic edible fungi, when culture medium is needed for assistance, the required culture medium is first prepared by a special preparation method, and then the organic edible fungi are inoculated into the culture medium. At this time, the culture medium provides nutrients for the organic edible fungi to reproduce, thereby promoting the growth of the organic edible fungi. However, in the preparation process of edible fungi culture medium, usually only dry heat sterilization is used, and the temperature and time are controlled to kill the microorganisms in the culture medium. This sterilization method cannot completely remove the microorganisms inside and outside the object, and then it is easy to cause incomplete sterilization of the culture medium, resulting in contamination of the culture medium with foreign bacteria, which in turn leads to unstable quality of the culture medium, affecting the culture efficiency, and thus affecting the use of the culture medium. Summary of the invention

[0004] The object of the present invention is to provide an organic edible fungus culture medium to solve the problems raised by the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: an organic edible fungus culture medium, the organic edible fungus culture medium comprising the following mass fractions: Water flow; 100g potatoes; Brown sugar 12g; Glucose 11g; 35g wheat bran; Peptone 1.8 g; Potassium dihydrogen phosphate 1.8g; Magnesium sulfate 0.8 g; Vitamin B1 tablet; Polyoxypropylene glycerin 0.35ml; Gibberellic acid 1.0g; 0.75g superphosphate.

[0006] An organic edible fungus culture medium, the preparation method comprising the following steps: S1. Raw material preparation: Prepare the required raw materials according to the selected formula to ensure the quality and purity of the raw materials; S2. PH determination and adjustment: Determine and adjust the pH value of the culture medium to ensure that the pH value of the culture medium is within the range suitable for microbial growth; S3, mixing and heating: crush the solid raw materials and mix them evenly, add appropriate amount of water, heat to boiling, and maintain for a certain time to ensure that the raw materials are fully dissolved or gelatinized; S4, Sterilization and cooling: The prepared culture medium is placed in a test tube or a conical flask, and then sterilized by high pressure to kill bacteria; after sterilization, the culture medium is cooled to a temperature suitable for inoculation; S5. Process inspection: Compare the manufactured culture medium with the control culture medium to determine whether the quality of the manufactured culture medium meets the requirements; S6. Inoculation and cultivation: Under sterile conditions, inoculate the edible fungus strains onto the culture medium and then culture them under appropriate temperature and humidity conditions.

[0007] Preferably, the solid raw materials in S1 are weighed using a weighing machine to ensure the balance of nutrients in the culture medium, and the water flow is distilled water with a resistivity of not less than 300,000 Ω.cm or water of the same quality.

[0008] Preferably, in S2, the pH value is measured by a precise pH meter in combination with pH test paper, and the pH is adjusted by 1 mol / L NaOH or 1 mol / L HCL.

[0009] Preferably, in S3, the mixing of the culture medium raw materials is completed by a stirrer, the mixing and stirring speed is 120 rpm to 200 rpm, and the mixing time is 1 min to 5 min.

[0010] Preferably, the heating in S3 is performed by a heating sterilization medium preparation device, the heating temperature is about 121 degrees, and the heating time is 20 minutes.

[0011] Preferably, in S4, a sterilization effect is achieved by adding a chemical sterilizer to the culture medium, and the chemical sterilizer is acetaldehyde or hydrogen peroxide, and subsequently the culture bottle containing the culture medium is irradiated with ultraviolet rays to improve the sterilization effect.

[0012] Preferably, the inspection process in S5 includes sterility testing and appearance testing, and a performance test is performed using a stable standard strain, and a comparative experiment is performed by observing the morphology, size, number and growth rate of the colonies to determine whether the quality of the culture medium meets the standards.

[0013] Preferably, in S6, the edible fungus strains are inoculated into the culture medium through an inoculation needle, and the culture temperature is between 20-30° C., and the culture humidity is maintained at 70%-80%.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: when the organic edible fungus culture medium is used, multiple batches of sterilization operations are performed to prevent the culture medium from being contaminated by microorganisms in the environment, and comparative tests and performance tests are performed to determine whether the quality of the culture medium meets the standards, thereby timely discovering whether the culture medium is contaminated by miscellaneous bacteria, thereby eliminating the influence of miscellaneous bacteria on the culture medium, preventing the reproduction of microorganisms in the culture medium from affecting the quality of the culture medium, and then providing a more suitable growth environment for edible fungi, thereby shortening the culture period of the organic edible fungus culture medium and improving the culture efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the preparation process operation of the present invention. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] See also Figure 1 The present invention provides a technical solution: an organic edible fungus culture medium, comprising the following mass fractions: water; 100g potato; 12g brown sugar; 11g glucose; 35g wheat bran; 1.8g peptone; 1.8g potassium dihydrogen phosphate; 0.8g magnesium sulfate; one vitamin B1 tablet; 0.35ml polyoxypropylene glycerol; 1.0g gibberellin; and 0.75g superphosphate.

[0018] A method for preparing an organic edible fungus culture medium comprises the following steps: S1. Raw material preparation: Prepare the required raw materials according to the selected formula to ensure the quality and purity of the raw materials; use a weighing machine to weigh the solid raw materials to ensure the balance of the nutrients in the culture medium, and the water flow is distilled water with a resistivity of not less than 300,000 Ω.cm or water of the same quality; potatoes are usually used as a carbon source and energy source in the culture medium to provide the energy required for the growth of edible fungi; brown sugar and glucose are both carbon sources, providing energy and carbon elements required for the growth of edible fungi; wheat bran is rich in dietary fiber and trace elements, providing necessary nutrition for edible fungi, and can regulate the physical properties of the culture medium, maintain an appropriate moisture content, and promote the growth of mycelium; white peptone contains organic nitrogen compounds, vitamins and sugars, and is a nitrogen source in the culture medium. Main ingredients: Potassium dihydrogen phosphate, as part of the phosphate buffer, can maintain the pH value of the culture medium and provide the necessary environmental conditions for the growth of edible fungi; magnesium sulfate provides magnesium and is a cofactor for a variety of enzymatic reactions; vitamin B1 participates in the formation of active groups of various enzymes, which play a key role in cell metabolism, and then regulates and controls cell metabolism; polyoxypropylene glycerol acts as a defoamer in the culture medium and can inhibit the generation of foam during the entire fermentation process; gibberellin, a natural growth regulator, has the effect of promoting cell division and proliferation in plant tissue culture; superphosphate in the culture medium can inhibit the growth of soil pathogens and fungi, and can also increase the water absorption and water retention of the seedling medium; S2. PH determination and adjustment: Determine and adjust the pH value of the culture medium to ensure that the pH value of the culture medium is within the range suitable for the growth of microorganisms; use a precision pH meter with pH test paper to determine the pH value, and adjust the pH with 1mol / LNa0H or 1mol / LHCL; use a precision pH meter to measure at 25°C, and do not adjust repeatedly to avoid affecting the osmotic pressure, and add Na0H or HCL drop by drop, while constantly stirring the culture medium, and then measure the pH value, so as to ensure the accuracy of the adjustment and avoid excessive addition; and before the measurement, make sure that the pH meter has been calibrated to ensure the accuracy of the measurement results; then insert the electrode of the pH meter into the culture medium, and wait for it to stabilize and read the pH value; S3, mixing and heating: crush the solid raw materials and mix them evenly, add appropriate amount of water, heat to boiling, and maintain for a certain time to ensure that the raw materials are fully dissolved or gelatinized; complete the mixing of the culture medium raw materials by a stirrer, the mixing and stirring speed is 120rpm~200rpm, and the mixing time is 1min~5min; heat by a heating sterilization culture medium preparation device, the heating temperature is about 121 degrees, and the heating time is 20 minutes; exhaust, pressure increase, pressure maintenance and pressure reduction operations are also required during the heating process; S4, Sterilization and cooling: The prepared culture medium is placed in a test tube or a conical flask, and then sterilized under high pressure to kill bacteria; after sterilization, the culture medium is cooled to a temperature suitable for inoculation; the sterilization effect is achieved by adding a chemical sterilizer to the culture medium, and the chemical sterilizer is acetaldehyde or hydrogen peroxide, and then the culture bottle containing the culture medium is irradiated with ultraviolet light to improve the sterilization effect; the culture medium is placed in a high temperature and high pressure resistant container, and then placed in a high pressure steam sterilizer, and the time and temperature are set for sterilization; the relative humidity of the environment during ultraviolet irradiation is 60%, the temperature is 20°C, and the ultraviolet intensity shall not be less than 70μW / cm^2, and the irradiation time shall be 24 minutes; S5. Process inspection: Compare the manufactured culture medium with the control culture medium to determine whether the quality of the manufactured culture medium meets the requirements; the inspection process includes sterility testing and appearance testing, and the performance test is carried out using a stable standard strain, and the comparative experiment is carried out by observing the morphology, size, number and growth rate of the colonies to determine whether the quality of the culture medium meets the standards; the appearance inspection mainly focuses on the color, transparency, odor, precipitation and impurities of the culture medium; a normal culture medium should be transparent, colorless or light yellow, without bubbles, precipitation or foreign matter; the observation of these appearance characteristics can preliminarily judge the quality of the culture medium; in the performance test, the growth of the colonies on the culture medium after inoculation is similar to that of the control culture medium and meets the expected colony characteristics, then it can be considered that the quality of the manufactured culture medium meets the requirements; sterility testing includes air sampling, high-pressure steam sterilizer effect testing, culture medium quality testing, utensils and equipment testing, and personnel hand testing to avoid microbial contamination and ensure the accuracy and reliability of the experimental results; S6. Inoculation and cultivation: Under sterile conditions, inoculate the edible fungi strains onto the culture medium, and then culture them under appropriate temperature and humidity conditions; use an inoculation needle to inoculate the edible fungi strains onto the culture medium, and the culture temperature is between 20-30°C, and the culture humidity is maintained at 70%-80%; before inoculation, the inoculation needle, inoculation loop and other tools need to be disinfected, and sterile operation must be maintained during inoculation to avoid contamination by foreign bacteria; and after inoculation, the culture medium is placed in a constant temperature box or culture room for cultivation; during the culture process, temperature, humidity, light, oxygen and other factors need to be controlled to meet the needs of the growth of the strains; at the same time, it is necessary to regularly observe the growth of the strains and adjust the culture conditions in time.

[0019] In specific implementation, in order to cultivate organic edible fungi, when it is necessary to prepare the culture medium in advance, first prepare the required raw materials according to the selected formula, then weigh them through a weighing machine, and ensure that the selected raw materials are of high quality and not contaminated. After completion, add distilled water for stirring, and add superphosphate to the culture medium to inhibit the growth of soil pathogens and fungi; then, in an environment of 25°C, calibrate the precision pH meter and insert it into the culture medium, wait for it to stabilize and read the pH value, because Na0H and HCL are strong acids or strong bases, when it is found that the pH value does not meet the requirements, add Na0H or HCL drop by drop, and stir the culture medium continuously at the same time, and then measure the pH value, and then ensure the accuracy of the adjustment to avoid excessive addition, until the pH value is 6 or 7, and it cannot be adjusted repeatedly to avoid affecting the osmotic pressure; Then, the raw materials of the culture medium are mixed by a stirrer, and the mixing speed is 180 rpm, and the mixing time is 3 minutes, so as to ensure that each raw material can be evenly distributed in the culture medium. After the mixing is completed, it is heated by a heating and sterilization culture medium preparation device, and the heating temperature is about 110 degrees, and the heating time is 18 minutes, until the raw materials are fully dissolved or gelatinized. This process is conducive to the release and uniform distribution of nutrients in the raw materials; hydrogen peroxide is then added to the culture medium for sterilization; thereafter, the prepared culture medium is filled into a test tube or a triangular flask, and then the culture medium is sterilized again by ultraviolet irradiation, and finally the culture medium is placed in a high temperature and high pressure resistant container, and then placed in a high pressure steam sterilizer to kill bacteria, and then through multiple batches of sterilization operations, the culture medium is prevented from being contaminated by microorganisms in the environment, and then the influence of miscellaneous bacteria on the culture medium can be eliminated, and the nutrient components of the culture medium are protected to provide a more suitable growth environment for organic edible fungi, thereby improving the culture efficiency; After the culture medium is cooled, compare the prepared culture medium with the control culture medium prepared in advance. First, the appearance inspection is mainly focused on the color, transparency, odor, precipitation and impurities of the culture medium; a normal culture medium should be transparent, colorless or light yellow, without bubbles, precipitation or foreign matter, and at the same time, it is compared with the control culture medium to preliminarily judge the quality of the culture medium, and then perform performance tests using stable standard strains, and conduct comparative experiments by observing the morphology, size, number and growth rate of the colonies to determine whether the quality of the culture medium meets the standards; then promptly discover whether the culture medium is contaminated by foreign bacteria, prevent foreign bacteria from multiplying in large numbers in the culture medium and consuming nutrients, and microorganisms will decompose amino acids and vitamins in the culture medium, affecting the quality of the culture medium.

[0020] Finally, under sterile conditions, the edible fungus strains are inoculated into the culture medium, and then placed in a constant temperature box or culture room for culture. The growth of the strains is observed regularly, and the culture conditions are adjusted in time. Thus, through stable sterilization treatment of multiple batches, the influence of miscellaneous bacteria on the culture medium is eliminated, and the reproduction of microorganisms in the culture medium is prevented from affecting the quality of the culture medium, thereby providing a more suitable growth environment for edible fungi, thereby shortening the culture cycle and improving the culture efficiency.

[0021] To sum up, when this organic edible fungus culture medium is used, after the required raw materials are prepared according to the selected formula, the pH value of the culture medium is first measured and adjusted to ensure that the pH value of the culture medium is within a range suitable for the growth of microorganisms, and then the solid raw materials are mixed and heated until the raw materials are fully dissolved or gelatinized, and then the prepared culture medium is placed in a test tube for sterilization, and then the culture medium is cooled to a temperature suitable for inoculation. This is a prior art and will not be elaborated on here. Through multiple batches of sterilization operations, the culture medium is prevented from being contaminated by microorganisms in the environment, and through comparative inspections and performance tests, it is timely discovered whether the culture medium is contaminated by miscellaneous bacteria, thereby providing a more suitable growth environment for edible fungi, thereby shortening the culture cycle of the organic edible fungus culture medium and improving the culture efficiency. The content not described in detail in this description belongs to the prior art known to professional and technical personnel in this field.

[0022] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An organic edible fungus culture medium, characterized in that: The organic edible fungus culture medium comprises the following parts by mass: Water flow; 100g potatoes; Brown sugar 12g; Glucose 11g; 35g wheat bran; Peptone 1.8 g; Potassium dihydrogen phosphate 1.8g; Magnesium sulfate 0.8 g; Vitamin B1 tablet; Polyoxypropylene glycerin 0.35ml; Gibberellic acid 1.0g; 0.75g superphosphate.

2. The organic edible fungus culture medium according to claim 1, characterized in that: The preparation method comprises the following steps: S1. Raw material preparation: Prepare the required raw materials according to the selected formula to ensure the quality and purity of the raw materials; S2. PH determination and adjustment: Determine and adjust the pH value of the culture medium to ensure that the pH value of the culture medium is within the range suitable for microbial growth; S3, mixing and heating: crush the solid raw materials and mix them evenly, add appropriate amount of water, heat to boiling, and maintain for a certain time to ensure that the raw materials are fully dissolved or gelatinized; S4, Sterilization and cooling: The prepared culture medium is placed in a test tube or a conical flask, and then sterilized by high pressure to kill bacteria; after sterilization, the culture medium is cooled to a temperature suitable for inoculation; S5. Process inspection: Compare the manufactured culture medium with the control culture medium to determine whether the quality of the manufactured culture medium meets the requirements; S6. Inoculation and cultivation: Under sterile conditions, inoculate the edible fungus strains onto the culture medium and then culture them under appropriate temperature and humidity conditions.

3. The organic edible fungus culture medium according to claim 1, characterized in that: The solid raw materials in S1 are weighed using a weighing machine to ensure the balance of nutrients in the culture medium, and the water flow is distilled water with a resistivity of not less than 300,000 Ω.cm or water of the same quality.

4. The organic edible fungus culture medium according to claim 1, characterized in that: In S2, the pH value is measured by a precise pH meter in combination with pH test paper, and the pH is adjusted by 1 mol / L NaOH or 1 mol / L HCL.

5. The organic edible fungus culture medium according to claim 1, characterized in that: In S3, the culture medium raw materials are mixed by a stirrer, the mixing and stirring speed is 120 rpm to 200 rpm, and the mixing time is 1 min to 5 min.

6. The organic edible fungus culture medium according to claim 1, characterized in that: In the S3, heating is performed by a heating sterilization medium preparation device, the heating temperature is about 121 degrees, and the heating time is 20 minutes.

7. The organic edible fungus culture medium according to claim 1, characterized in that: In S4, a chemical sterilizer is added to the culture medium to achieve a sterilization effect, and the chemical sterilizer is acetaldehyde or hydrogen peroxide, and then the culture bottle containing the culture medium is irradiated with ultraviolet rays to improve the sterilization effect.

8. The organic edible fungus culture medium according to claim 1, characterized in that: The inspection process in S5 includes sterility inspection and appearance inspection, and the performance test is carried out by using a stable standard strain, and the comparative experiment is carried out by observing the morphology, size, number and growth rate of the colonies to determine whether the quality of the culture medium meets the standards.

9. The organic edible fungus culture medium according to claim 1, characterized in that: In S6, the edible fungus strains are inoculated into the culture medium through an inoculation needle, and the culture temperature is between 20-30° C. and the culture humidity is maintained at 70%-80%.

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