Polyphenol poultry feed and preparation method thereof

Abstract

The invention discloses a polyphenol poultry feed and a preparation method thereof, and belongs to the field of feeds. The polyphenol poultry feed comprises a feed main component and polyphenol lettuce freeze-dried powder, and the feed main component comprises the following raw materials in parts by weight: 8-15 parts of corn; 3-7 parts of soybean meal; 2-5 parts of extruded soybeans; 0.3 to 0.7 part of stone powder; 0.7 to 1.3 parts of a premix; the dosage of the polyphenol lettuce freeze-dried powder is 0.05-0.15% of the mass of the main components of the feed. The preparation method of the polyphenol poultry feed comprises the following steps: mixing and stirring the corn, the soybean meal, the extruded soybeans, the stone powder, the premix and the polyphenol lettuce freeze-dried powder to obtain the polyphenol poultry feed. The method has the advantages that potential safety hazards are reduced, and meanwhile the production capacity of laying fowls is improved.

Description

Technical Field

[0001] The present application relates to the field of feed, and in particular to a polyphenol poultry feed and a preparation method thereof. Background Art

[0002] Poultry feed includes chicken feed, duck feed, goose feed and other types. It has a direct impact on the growth, development and production performance of poultry, and provides nutrition and energy. The quality and formula of poultry feed directly affect the health of poultry, ensuring that poultry obtains comprehensive and balanced nutrition. It can also reduce the risk of disease in poultry, optimize breeding technology, and improve production efficiency, thereby improving the economic benefits of breeding.

[0003] The nutritional requirements of poultry vary depending on the species, age, physiological state and production purpose. For example, egg-laying poultry and meat poultry have different nutritional requirements. Meat poultry usually have higher requirements for protein and fat, while egg-laying poultry have higher requirements for calcium and phosphorus. For egg-laying poultry, stone powder needs to be added to the feed as a calcium source.

[0004] In addition, based on the egg-laying needs of laying poultry, hormone additives are directly added to some feeds to control the growth and reproduction of laying poultry and improve the egg-laying level of laying poultry. However, the addition of hormone additives may have a negative impact on the health of laying poultry. If there are too many hormone residues, it may pose a threat to human health and pose a safety hazard. Summary of the invention

[0005] In order to improve the production capacity of laying fowls while reducing potential safety hazards, the present application provides a polyphenol poultry feed and a preparation method thereof.

[0006] In the first aspect, the polyphenol poultry feed provided by the present application adopts the following technical solution: A polyphenol poultry feed comprises a feed main component and polyphenol lettuce freeze-dried powder, wherein the feed main component comprises the following raw materials in parts by weight: 8-15 servings of corn; 3-7 parts soybean meal; 2-5 portions of puffed soybeans; 0.3-0.7 parts of stone powder; Premix 0.7-1.3 parts; The amount of the polyphenol lettuce freeze-dried powder is 0.05-0.15% of the mass of the main component of the feed.

[0007] By adopting the above technical scheme, polyphenol lettuce freeze-dried powder can provide rich polyphenols, such as quercetin, chlorogenic acid, anthocyanin and chicoric acid polyphenols. Polyphenol lettuce freeze-dried powder is used in feed to provide good antioxidant effect, reduce the level of cell oxidation stress, protect the reproductive cells and tissues in the poultry body from damage, and promote the absorption of feed nutrients by poultry. It can also promote the secretion of female hormones and improve stress indicators by regulating the metabolic process in the poultry body. Therefore, the comprehensive effect improves the reproductive capacity of poultry, maintains the microbial indicators of the poultry intestine at a good level, prolongs the peak egg-laying period, and improves economic benefits.

[0008] In addition, the source of polyphenol lettuce freeze-dried powder is natural and harmless, and no hormone additives are directly added, which effectively avoids the risk of increasing the burden on poultry viscera and reduces the harm caused to humans eating corresponding poultry foods, making it healthier and safer.

[0009] Optionally, the source material of the polyphenol lettuce freeze-dried powder is polyphenol lettuce, and the total polyphenol content of the polyphenol lettuce is 2 to 6 mg / g.

[0010] By adopting the above technical scheme, the polyphenol content of general lettuce on the market is generally less than 1 mg / g, and the polyphenol content in polyphenol lettuce is much higher than that of ordinary lettuce, especially high content of polyphenols such as quercetin, chlorogenic acid, anthocyanin and chicoric acid. The freeze-dried polyphenol lettuce powder is added to the feed for poultry to eat, so that poultry and livestock can obtain richer nutrition. Compared with the method of directly adding artificially refined quercetin extract and chlorogenic acid extract, the organic combination of polyphenol lettuce freeze-dried powder improves the absorption of polyphenol nutrition by poultry, thereby having more obvious advantages in reproductive functions such as egg-laying ability.

[0011] Optionally, the preparation method of the polyphenol lettuce freeze-dried powder is as follows: freeze-dry the polyphenol lettuce in a vacuum environment at -20 to -50°C for 16 to 30 hours, and grind the polyphenol lettuce to obtain the polyphenol lettuce freeze-dried powder.

[0012] By adopting the above technical scheme, freeze-drying under vacuum can retain the nutritional components of polyphenol lettuce to the greatest extent, especially the heat-sensitive polyphenols can be well retained. After vacuum freeze-drying, the storage time of polyphenol lettuce is prolonged and it can withstand relatively harsh environments. Therefore, it is suitable for adding to feed and exerting the corresponding polyphenol effects for a long time.

[0013] Optionally, the particle size of the polyphenol lettuce freeze-dried powder is 200-250 μm.

[0014] By adopting the above technical solution, the polyphenol lettuce freeze-dried powder of the above particle size can balance the nutritional supplementation and preservation capacity of the feed, thereby improving the overall effect.

[0015] Optionally, the preparation method of the puffed soybeans includes: peeling and crushing the soybeans to obtain soybean powder, and conditioning, puffing, and crushing the soybean powder with steam to obtain puffed soybeans.

[0016] By adopting the above technical solution, soybeans are rich in nutrients and have good palatability after conditioning and puffing, and are suitable for adding to poultry feed to promote poultry digestion and absorption.

[0017] Optionally, the puffed soybeans are polyphenol puffed soybeans, and the preparation method of the polyphenol puffed soybeans is as follows: the soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and then puffed for the first time, and polyphenol lettuce freeze-dried powder is added after puffing, and then puffed for a second time and crushed to obtain puffed soybeans.

[0018] By adopting the above technical scheme, polyphenol lettuce freeze-dried powder is added during the preparation of puffed soybeans to achieve the adjustment of the nutritional components of puffed soybeans, exert a more sufficient absorption-promoting effect, and perform secondary puffing. The polyphenol lettuce freeze-dried powder is added between the secondary puffing to fully combine the polyphenol lettuce freeze-dried powder with the soybeans without reducing the nutritional effect of the polyphenol lettuce freeze-dried powder due to excessive puffing. In addition to improving the egg quality of poultry, the eggshell strength is increased and the stress index is further improved.

[0019] Optionally, the premix is ​​a premix containing trace elements.

[0020] By adopting the above technical solution, the premix can provide trace element nutrients for poultry as an additional supplementary ingredient.

[0021] Optionally, the particle size of the stone powder is 25 to 50 μm.

[0022] By adopting the above technical solution, the dispersibility and stability of stone powder in feed are guaranteed, and at the same time, necessary calcium is provided to meet the needs of poultry, especially laying poultry.

[0023] In a second aspect, the present application provides a method for preparing a polyphenol poultry feed using the following technical solution: A method for preparing a polyphenol poultry feed comprises the following steps: The corn, soybean meal, puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are mixed and stirred to obtain polyphenol poultry feed.

[0024] In summary, this application has the following beneficial effects: 1. The polyphenol lettuce freeze-dried powder selected in this application can provide rich polyphenols, such as quercetin, chlorogenic acid, anthocyanin and chicoric acid polyphenols. The polyphenol lettuce freeze-dried powder is used in feed to provide good antioxidant effects, reduce the stress level of cell oxidation, protect the reproductive cells and tissues in the poultry from damage, and promote the absorption of feed nutrients by poultry. It can also promote the secretion of female hormones by regulating the metabolic process in the poultry, so it improves the reproductive capacity of poultry in terms of comprehensive effects, especially the egg-laying capacity of laying poultry. In addition, the source of polyphenol lettuce freeze-dried powder is natural and harmless, and hormone additives are not directly added, which effectively avoids the risk of increasing the burden on poultry viscera, and also reduces the harm caused by human consumption of corresponding poultry food, which is healthier and safer.

[0025] 2. Polyphenol lettuce freeze-dried powder is added to feed for poultry to eat, so that poultry and livestock can obtain richer nutrition. Compared with the method of directly adding artificially extracted quercetin extract and chlorogenic acid extract, the organic combination of polyphenol lettuce freeze-dried powder improves the absorption of polyphenol nutrition by poultry, thus having more obvious advantages in reproductive functions such as egg-laying ability.

[0026] 3. Polyphenol lettuce freeze-dried powder is added during the preparation of puffed soybeans to achieve the adjustment of the nutritional components of puffed soybeans, play a more sufficient absorption-promoting role, and perform secondary puffing. The polyphenol lettuce freeze-dried powder is added between the secondary puffing to fully combine the polyphenol lettuce freeze-dried powder with soybeans without reducing the nutritional effect of the polyphenol lettuce freeze-dried powder due to excessive puffing. In addition to increasing the egg production of poultry, it can also improve the quality of egg production and maintain the microbial indicators of the poultry intestine at a good level. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a bar graph showing reproductive hormone levels.

[0028] Figure 2 This is a bar graph showing the detection of stress hormone levels.

[0029] Figure 3 The bar graph shows the abundance of different microorganisms. DETAILED DESCRIPTION

[0030] The present application is described in further detail below.

[0031] The polyphenol lettuce was purchased from Zhejiang Jiameishan Plant Technology Co., Ltd. The total polyphenol content of the polyphenol lettuce was 2-6 mg / g. The polyphenol lettuce used in the following preparation example was tested and the total polyphenol content was 2 mg / g.

[0032] Preparation Example 1 Preparation method of polyphenol lettuce freeze-dried powder: Select polyphenol lettuce, wash it with water, place the washed polyphenol lettuce in a vacuum freeze dryer at -20°C for freeze drying, freeze drying for 30 hours, grind the polyphenol lettuce after freeze drying to obtain polyphenol lettuce freeze dried powder. The particle size of the polyphenol lettuce freeze dried powder is 200-250 mm, and the total polyphenol content of the polyphenol lettuce freeze dried powder is 38.55 mg / g, of which the main polyphenol types are quercetin 12.18 mg / g, anthocyanin 6.79 mg / g, isochlorogenic acid B 4.56 mg / g, and chicoric acid 6.80 mg / g.

[0033] Preparation Example 2 Preparation method of polyphenol lettuce freeze-dried powder: Select polyphenol lettuce, wash it with water, place the washed polyphenol lettuce in a vacuum freeze dryer at -50°C for freeze drying, freeze drying for 16 hours, grind the polyphenol lettuce after freeze drying to obtain polyphenol lettuce freeze-dried powder. The particle size of the polyphenol lettuce freeze-dried powder is 200-250 mm, and the total polyphenol content of the polyphenol lettuce freeze-dried powder is 41.27 mg / g, of which the main polyphenol types are quercetin 13.58 mg / g, anthocyanin 6.69 mg / g, isochlorogenic acid B 4.74 mg / g, and chicoric acid 6.51 mg / g.

[0034] Comparative Preparation Example 1 Preparation method of lettuce freeze-dried powder: Ordinary lettuce was selected. After sampling and testing, the total polyphenol content of the ordinary lettuce was 0.7 mg / g.

[0035] Wash with water, place the washed common lettuce in a vacuum freeze dryer at -50°C for freeze drying, freeze drying for 16 hours, grind the common lettuce after freeze drying to obtain common lettuce freeze dried powder. The particle size of the common lettuce freeze dried powder is 200-250 mm, and the total polyphenol content of the common lettuce freeze dried powder is 12.40 mg / g, of which the main polyphenol types are quercetin 3.08 mg / g, anthocyanin 1.41 mg / g, isochlorogenic acid B 0.90 mg / g, and chicoric acid 1.53 mg / g.

[0036] Example 1 A method for preparing a polyphenol poultry feed comprises the following steps: Weigh 15kg of corn, 7kg of soybean meal, 5kg of puffed soybeans, 0.7kg of stone powder, 1.3kg of premix, and 14.5g of polyphenol lettuce freeze-dried powder.

[0037] The preparation method of puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and puffed, the material after puffing has a temperature of 110°C and a moisture content of 10%, and is crushed to obtain puffed soybeans.

[0038] The particle size of stone powder is 25~50μm The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0039] The polyphenol lettuce freeze-dried powder was prepared by Preparation Example 2.

[0040] Corn, soybean meal, puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are put into a mixer and mixed for 2 minutes, and the material is discharged to obtain polyphenol poultry feed.

[0041] Example 2 A method for preparing a polyphenol poultry feed comprises the following steps: Weigh 15kg of corn, 7kg of soybean meal, 5kg of puffed soybeans, 0.7kg of stone powder, 1.3kg of premix, and 29g of polyphenol lettuce freeze-dried powder.

[0042] The preparation method of puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and puffed, the material after puffing has a temperature of 110°C and a moisture content of 10%, and is crushed to obtain puffed soybeans.

[0043] The particle size of the stone powder is 25 to 50 μm.

[0044] The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0045] The polyphenol lettuce freeze-dried powder was prepared by Preparation Example 2.

[0046] Corn, soybean meal, puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are put into a mixer and mixed for 2 minutes, and the material is discharged to obtain polyphenol poultry feed.

[0047] Example 3 A method for preparing a polyphenol poultry feed comprises the following steps: Weigh 15kg of corn, 7kg of soybean meal, 5kg of puffed soybeans, 0.7kg of stone powder, 1.3kg of premix, and 43.5g of polyphenol lettuce freeze-dried powder.

[0048] The preparation method of puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and puffed, the material after puffing has a temperature of 110°C and a moisture content of 10%, and is crushed to obtain puffed soybeans.

[0049] The particle size of stone powder is 25~50μm The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0050] The polyphenol lettuce freeze-dried powder was prepared by Preparation Example 2.

[0051] Corn, soybean meal, puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are put into a mixer and mixed for 2 minutes, and the material is discharged to obtain polyphenol poultry feed.

[0052] Example 4 A method for preparing a polyphenol poultry feed comprises the following steps: Weigh 8kg of corn, 3kg of soybean meal, 2kg of puffed soybeans, 0.3kg of stone powder, 0.7kg of premix, and 7g of polyphenol lettuce freeze-dried powder.

[0053] The preparation method of puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and puffed, the material after puffing has a temperature of 110°C and a moisture content of 10%, and is crushed to obtain puffed soybeans.

[0054] The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0055] The polyphenol lettuce freeze-dried powder was prepared by Preparation Example 1.

[0056] Corn, soybean meal, puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are put into a mixer and mixed for 2 minutes, and the material is discharged to obtain polyphenol poultry feed.

[0057] Example 5 A method for preparing a polyphenol poultry feed comprises the following steps: Weigh 15kg of corn, 7kg of soybean meal, 5kg of polyphenol puffed soybeans, 0.7kg of stone powder, 1.3kg of premix, and 43.5g of polyphenol lettuce freeze-dried powder.

[0058] The preparation method of polyphenol puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and then puffed for the first time, the material after puffing has a discharge temperature of 110°C and a discharge moisture of 18wt%, the polyphenol lettuce freeze-dried powder prepared in Preparation Example 2 is added to the discharged soybean material, the polyphenol lettuce freeze-dried powder accounts for 0.1% of the weight of the soybean material, the mixture is evenly mixed, and then puffed for the second time, the material has a discharge temperature of 130°C and a discharge moisture of 12wt%, and finally crushed to obtain puffed soybeans.

[0059] The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0060] The polyphenol lettuce freeze-dried powder was prepared by Preparation Example 2.

[0061] Corn, soybean meal, polyphenol puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are put into a mixer and mixed for 2 minutes, and the material is discharged to obtain polyphenol poultry feed.

[0062] Example 6 A method for preparing a polyphenol poultry feed comprises the following steps: Weigh 15kg of corn, 7kg of soybean meal, 5kg of polyphenol puffed soybeans, 0.7kg of stone powder, 1.3kg of premix, and 43.5g of polyphenol lettuce freeze-dried powder.

[0063] The preparation method of polyphenol puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, the polyphenol lettuce freeze-dried powder prepared in Preparation Example 2 is added to the tempered soybean powder, the polyphenol lettuce freeze-dried powder accounts for 0.1% of the weight of the soybean material, and the mixture is evenly mixed, and then the first puffing is performed, and the material after puffing has a temperature of 110° C. and a moisture content of 18wt%; the second puffing is performed, and the material has a temperature of 130° C. and a moisture content of 12wt%; and finally the powder is crushed to obtain puffed soybeans.

[0064] The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0065] The polyphenol lettuce freeze-dried powder was prepared by Preparation Example 2.

[0066] Corn, soybean meal, polyphenol puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are put into a mixer and mixed for 2 minutes, and the material is discharged to obtain polyphenol poultry feed.

[0067] Comparative Example 1 A method for preparing poultry feed comprises the following steps: Weigh 15kg corn, 7kg soybean meal, 5kg puffed soybeans, 0.7kg stone powder, and 1.3kg premix.

[0068] The preparation method of puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and puffed, the material after puffing has a temperature of 110°C and a moisture content of 10%, and is crushed to obtain puffed soybeans.

[0069] The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0070] Corn, soybean meal, puffed soybean, stone powder and premix are put into a mixer and mixed for 2 minutes, and then the materials are discharged to obtain poultry feed.

[0071] Comparative Example 2 A method for preparing poultry feed comprises the following steps: Weigh 15 kg of corn, 7 kg of soybean meal, 5 kg of puffed soybeans, 0.7 kg of stone powder, 1.3 kg of premix, and 14.5 g of ordinary lettuce freeze-dried powder prepared in Comparative Preparation Example 1.

[0072] The preparation method of puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and puffed, the material after puffing has a temperature of 110°C and a moisture content of 10%, and is crushed to obtain puffed soybeans.

[0073] The premix is ​​a trace element premix, and the trace element premix contains 6.58 mg / kg copper, 476.20 mg / kg iron, 80.25 mg / kg manganese, 27.05 mg / kg zinc, and 0.0698 mg / kg selenium.

[0074] Ordinary lettuce freeze-dried powder was prepared by Comparative Preparation Example 1.

[0075] Corn, soybean meal, puffed soybean, stone powder, premix and common lettuce freeze-dried powder were put into a mixer and mixed for 2 minutes, and then the materials were discharged to obtain poultry feed.

[0076] Feed feeding effect 240 Hy-Line brown young chickens (7 months old) were selected as experimental chickens and randomly divided into 8 groups, each with 30 chickens. Each group was fed with the feed of Examples 1-6 and Comparative Examples 1-2, respectively, with a daily feed dosage of 110 g. The experimental chickens were fed to the 90th day for reproductive hormone detection, stress hormone detection, yolk follicle statistics and intestinal microbial detection. In addition, the eggs laid by the experimental chickens were taken for eggshell strength detection, live embryo rate statistics and hatching rate statistics. The eggs were specifically selected from the eggs laid closest to the 85th to 90th days.

[0077] Reproductive hormone testing: Reproductive hormone testing includes estradiol content testing and progesterone content testing. The average value of each group of data is taken. The results are shown in Table 1 and Figure 1 As shown, Figure 1In the figure, “0.05% purple phenol addition” represents Example 1, “0.1% purple phenol addition” represents Example 2, “0.15% purple phenol addition” represents Example 3, and “control” represents Comparative Example 1.

[0078] Stress hormone detection: Stress hormone detection includes corticosterone content detection, and the average value of each group of data is taken. The results are shown in Table 1 and Figure 2 As shown, Figure 2 In the figure, “0.05% purple phenol addition” represents Example 1, “0.1% purple phenol addition” represents Example 2, “0.15% purple phenol addition” represents Example 3, and “control” represents Comparative Example 1.

[0079] Table 1 Estradiol content (pg / mL) Progesterone content (pg / mL) Corticosterone content (pg / mL) Example 1 1413.42 949.91 23.33 Example 2 5877.90 3916.94 46.55 Example 3 9555.22 7259.39 41.62 Example 4 1523.54 966.87 22.68 Example 5 10352.34 7836.68 32.55 Example 6 9654.69 7035.36 42.13 Comparative Example 1 959.03 775.53 56.80 Comparative Example 2 1023.58 790.24 57.93 From Table 1 and Figure 1 It can be analyzed that after feeding the feed containing freeze-dried polyphenol lettuce powder, the estradiol and progesterone contents in the test chickens increased significantly, indicating that the secretion of reproductive hormones was promoted, which can prolong the peak egg-laying period, increase egg production, and obtain higher economic benefits.

[0080] Secondly, from Table 1 and Figure 2 It can be analyzed that corticosterone is a hormone secreted under stress. After feeding the feed containing freeze-dried polyphenol lettuce powder, the corticosterone content in the experimental chickens was significantly reduced, indicating that the stress response of the experimental chickens was alleviated, making it easier to lay eggs smoothly, increasing egg production and improving egg quality.

[0081] In addition, as the amount of polyphenol lettuce freeze-dried powder added to the feed increases, the secretion of reproductive hormones increases, and the secretion of corticosterone also increases. See Example 5. Adding polyphenol lettuce freeze-dried powder between the puffing steps of puffed soybeans can effectively improve the problem of increased corticosterone secretion and make the egg-laying process of laying hens smoother.

[0082] Statistics of small yolk follicles: Small yolk follicles with a diameter of 6-8 mm in the test chickens were taken out and counted. The average value of each group of data was taken. The results are shown in Table 2.

[0083] Eggshell strength test: Use an eggshell strength tester to test the eggshell strength of eggs, unit: kg / cm 2 , the average value of each group of data is taken, and the results are shown in Table 2.

[0084] Statistics of live embryo rate: The live embryo rate is calculated by dividing the number of eggs in each group by the number of eggs incubated. The results are shown in Table 2.

[0085] Statistics of hatching rate: The hatching rate was calculated by dividing the number of hatched chicks in each group by the number of fertilized eggs. The results are shown in Table 2.

[0086] Table 2 From Table 2, it can be analyzed that after feeding the feed containing freeze-dried polyphenol lettuce powder, the number of small yellow follicles in the test chickens increased significantly, the eggshell strength of the eggs produced also increased, and the live embryo rate and hatching rate increased, indicating that freeze-dried polyphenol lettuce powder has an improving effect on egg production quality.

[0087] Intestinal microbial detection: The abundance of different intestinal microorganisms of the experimental chickens fed in Examples 1-2 and Comparative Example 1 was obtained by microbial sequencing. The results are as follows: Figure 3 As shown, Figure 2 In the table, “0.05%” represents Example 1, “0.1%” represents Example 2, and “control” represents Comparative Example 1.

[0088] The microbial sequencing workflow includes the following steps: DNA extraction: After the genomic DNA extraction is completed, the extracted genomic DNA is detected by 1% agarose gel electrophoresis.

[0089] PCR amplification: Synthesize specific primers with barcodes according to the specified sequencing region. To ensure the accuracy and reliability of subsequent data analysis, two conditions must be met: 1) Use low cycle amplification as much as possible; 2) Ensure that the number of cycles for each sample is consistent. Randomly select representative samples for preliminary experiments to ensure that the vast majority of samples can amplify products of appropriate concentrations at low cycle numbers. Fluorescence quantification: Based on the preliminary quantitative results of electrophoresis, the PCR products were quantified using QuantiFluor TM -ST blue fluorescence quantitative system for detection and quantification, and then mix in corresponding proportions according to the sequencing quantity requirements of each sample.

[0090] Library construction: 1) Add the adapter sequence to the outer end of the target region by PCR; 2) Use a gel recovery kit to excise the gel and recover the PCR product; 3) Elute with Tris-HCl buffer and detect with 2% agarose gel electrophoresis; 4) Denature with sodium hydroxide to generate single-stranded DNA fragments.

[0091] Sequencing on the machine: 1) The linker sequence of the DNA fragment is complementary to the base sequence embedded on the chip and fixed on the chip; 2) The DNA fragment is used as a template and the base sequence fixed on the chip is used as a primer for PCR synthesis to synthesize the target DNA fragment to be tested on the chip; 3) After denaturation and annealing, the other end of the DNA fragment on the chip is randomly complementary to another nearby primer and is also fixed to form a "bridge"; 4) PCR amplification to produce DNA clusters; 5) The DNA amplicon is linearized into a single strand; 6) The modified DNA polymerase and dNTP with 4 fluorescent labels are added, and only one base is synthesized in each cycle; 7) The surface of the reaction plate is scanned with a laser to read the types of nucleotides polymerized by each template sequence in a round of reaction; 8) The "fluorescent group" and "termination group" are chemically cut to restore the stickiness of the 3' end and continue to polymerize nucleotides; 9) The fluorescence signal results collected in each round are counted to obtain the sequence of the template DNA fragment.

[0092] Bioinformatics analysis: After the PEreads obtained by sequencing are split into samples, the paired-end reads are first quality controlled and filtered according to the sequencing quality, and then spliced ​​according to the overlap relationship between the paired-end reads to obtain the optimized data after quality control splicing. Then, the sequence noise reduction method (DADA2 / Deblur, etc.) is used to process the optimized data to obtain ASV (Amplicon Sequence Variant) representing the sequence and abundance information.

[0093] from Figure 3 It can be analyzed that the relative abundance of Bacteroides (the main intestinal symbiotic bacteria) and Oscilospiraceae (conditional pathogens) decreased significantly, while the relative abundance of Lachnospira, Desulfovibrio, Christensenellaceae R-7group, Olsenella (beneficial bacteria), Romboutsia, Butyricicoccus (butyrate-producing), Peptococcus, Turicibacter (beneficial bacteria), and Blautia (beneficial bacteria) increased significantly, indicating that the abundance of beneficial bacteria increased, harmful bacteria decreased, and laying hens absorbed better nutrients.

[0094] This specific implementation manner is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the specific implementation manner as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A polyphenol poultry feed, characterized in that: It comprises a main feed component and polyphenol lettuce freeze-dried powder, wherein the main feed component comprises the following raw materials in parts by weight: 8-15 servings of corn; 3-7 parts soybean meal; 2-5 portions of puffed soybeans; 0.3~0.7 parts of stone powder; Premix 0.7~1.3 parts; The amount of the polyphenol lettuce freeze-dried powder is 0.05-0.15% of the mass of the main component of the feed.

2. The polyphenol poultry feed according to claim 1, characterized in that: The source material of the polyphenol lettuce freeze-dried powder is polyphenol lettuce, and the total polyphenol content of the polyphenol lettuce is 2-6 mg / g.

3. The polyphenol poultry feed according to claim 1, characterized in that: The preparation method of the polyphenol lettuce freeze-dried powder comprises the following steps: freeze-drying the polyphenol lettuce in a vacuum environment at -20 to -50°C for 16 to 30 hours, and crushing the polyphenol lettuce to obtain the polyphenol lettuce freeze-dried powder.

4. The polyphenol poultry feed according to claim 1, characterized in that: The particle size of the polyphenol lettuce freeze-dried powder is 200-250 μm.

5. The polyphenol poultry feed according to claim 1, characterized in that: The preparation method of the puffed soybeans comprises the following steps: peeling and crushing the soybeans to obtain soybean powder, and using steam to condition, puff and crush the soybean powder to obtain the puffed soybeans.

6. The polyphenol poultry feed according to claim 1, characterized in that: The puffed soybeans are polyphenol puffed soybeans. The preparation method of the polyphenol puffed soybeans is as follows: soybeans are peeled and crushed to obtain soybean powder, the soybean powder is tempered with steam, and then puffed for the first time, polyphenol lettuce freeze-dried powder is added after puffing, and then puffed for the second time and crushed to obtain puffed soybeans.

7. The polyphenol poultry feed according to claim 1, characterized in that: The premix is ​​selected from premixes containing trace elements.

8. The polyphenol poultry feed according to claim 1, characterized in that: The particle size of the stone powder is 25-50 μm.

9. A method for preparing the polyphenol poultry feed according to any one of claims 1 to 8, characterized in that: The following steps are involved: The corn, soybean meal, puffed soybean, stone powder, premix and polyphenol lettuce freeze-dried powder are mixed and stirred to obtain polyphenol poultry feed.

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