Composite gel health-care food for reducing blood fat and blood pressure and preparation method of composite gel health-care food

Abstract

The invention belongs to the field of health-care food preparation, and particularly relates to a composite gel health-care food for reducing blood fat and blood pressure and a preparation method of the composite gel health-care food. The composite gel health-care food is composed of a capsule shell and a content, the content is prepared into Pickering emulsion, the content comprises solid particles, a plant mixture and a water-soluble compound, the solid particles are specifically a soybean protein-porphyra yezoensis polysaccharide-astaxanthin compound and can replace a traditional surface activating agent to play an emulsifying role, and the plant mixture can serve as a plant mixture and a water-soluble compound. The plant mixture is obtained by mixing the sea buckthorn seed oil and the plant extract, and the plant extract is obtained by extracting hawthorn, kudzuvine root, gynostemma pentaphylla, pepper and white hyacinth bean by adopting a deep eutectic solvent in combination with distillation, so that the health level of blood fat and blood pressure can be maintained; the water-soluble complex contributes to emulsion stabilization of the contents. The composite gel health-care food prepared by the preparation method disclosed by the invention is relatively high in safety and has relatively good health-care effects of maintaining blood fat and blood pressure at a healthy level.

Description

Technical Field

[0001] The invention belongs to the field of health food preparation, and in particular relates to a composite gel health food for lowering blood lipids and blood pressure and a preparation method thereof. Background Art

[0002] Cardiovascular diseases seriously endanger human health. Among the factors that lead to cardiovascular diseases, hypertension and hyperlipidemia are two risk factors that have received a lot of attention. Hyperlipidemia refers to elevated triglycerides and / or total cholesterol in plasma, and also includes elevated low-density lipoprotein cholesterol and decreased high-density lipoprotein cholesterol. Hyperlipidemia can cause atherosclerotic cardiovascular disease, pancreatitis, fatty liver and other diseases. Hypertension refers to a clinical syndrome characterized by increased systemic arterial blood pressure (systolic and / or diastolic blood pressure) (systolic blood pressure ≥ 140 mmHg, diastolic blood pressure ≥ 90 mmHg), which may be accompanied by functional or organic damage to organs such as the heart, brain, and kidneys. Common symptoms include dizziness, headache, stiff neck, fatigue, palpitations, etc.

[0003] Hyperlipidemia can aggravate hypertension. When blood lipid levels increase, they can enter the blood vessel walls and accumulate, forming atherosclerotic plaques, narrowing the blood vessel lumen, increasing blood flow resistance, and increasing blood pressure. Hypertension promotes the accumulation of blood lipids in the blood vessel walls. Hypertension can easily cause damage to the vascular endothelium, creating opportunities for blood lipids to enter the blood vessel walls and accelerating the process of atherosclerosis. The coexistence of hyperlipidemia and hypertension will accelerate the process of atherosclerosis and significantly increase the risk of cardiovascular disease. Therefore, it is very necessary to pay attention to these two high-risk factors in daily health management. Controlling hyperlipidemia and hypertension can effectively prevent and treat cardiovascular diseases.

[0004] There are many types of antihypertensive drugs, mainly calcium antagonists, diuretics, beta-blockers, angiotensin converting enzyme inhibitors, etc. Among them, calcium antagonists occupy a prominent position and can be used to treat hypertension of varying severity. Statins, fibrates, niacin, bile acid chelators, etc. are the lipid-lowering drugs mainly used in clinical practice at this stage. Regardless of whether drug treatment is initiated, a healthy lifestyle can prevent or delay the occurrence and development of cardiovascular diseases. Health foods with certain functions have received more attention. Their advantages are that they are easy to take and harmless to the human body. They mainly achieve certain functionality through prevention and regulation. Compared with drugs with clear therapeutic effects, health foods are easier for people to accept and have less psychological burden. Summary of the invention

[0005] Based on the above problems, the present invention provides a composite gel health food for lowering blood lipids and blood pressure, wherein the contents are prepared into a Pickering emulsion, and the contents include solid particles, a plant mixture and a water-soluble complex, and the solid particles are specifically a soy protein-porphyra yezoensis polysaccharide-astaxanthin complex, which can replace the traditional surfactant to play an emulsifying role, and at the same time cooperate with the plant mixture to play a health care effect of maintaining the balance of blood lipids and blood pressure. The plant extract in the plant mixture is obtained by extracting hawthorn, kudzu root, gynostemma pentaphyllum, prickly ash and white hyacinth bean, and further extracted by using a deep eutectic solvent combined with distillation, and the plant extract finally obtained is used as a core functional substance to achieve the health care effect of maintaining a healthy level of blood lipids and blood pressure. The composite gel health food prepared by the present invention has high safety and has a good health care effect of maintaining blood lipids and blood pressure at a healthy level.

[0006] The present invention provides a composite gel health food for lowering blood lipid and blood pressure, which is composed of two parts: content and capsule shell; The contents include the following raw materials in parts by mass: 3-5 parts of solid particles, 8-12 parts of plant mixtures, and 50-60 parts of water-soluble complexes.

[0007] The solid particles are a soybean protein-porphyra yezoensis polysaccharide-astaxanthin complex, and the specific preparation method is as follows: L1. Dissolve soy protein in water, stir at room temperature at 150-200 rpm for 30-45 min, store at 4 ° C for 10-12 h, adjust pH to 10.0, heat to 80-90 ° C and maintain for 20-30 min to obtain substance 1; L2. The dried Porphyra yezoensis was ground into powder and then sieved, water was added, and the bacterial suspension was added, and the fermentation was continued at 28-30°C for 24-28 hours. After the fermentation, the supernatant was sterilized at high temperature to obtain substance 2; L3. Mix the substance 1 obtained in step L1 and the substance 2 obtained in step L2 in a volume ratio of 1:0.8-1.5, adjust the pH to 4.0, and continue stirring at a speed of 280-320 rpm at room temperature for 20-30 minutes to obtain substance 3. Add astaxanthin powder and continue stirring for 45-60 minutes. After completion, freeze-dry for 45-50 hours to obtain solid particles.

[0008] Preferably, in step L1, the mass volume ratio of soy protein to water is 1:50-60.

[0009] Preferably, the sieving in step L2 is preferably 60-80 mesh, the mass ratio of Porphyra yezoensis powder: water: bacterial suspension is 1: 8-10: 0.1-0.3, the bacterial suspension is obtained after activation of Lactobacillus plantarum, the Lactobacillus plantarum is from the China General Microbial Culture Collection Center, the strain number is CGMCC No.1.573, and the concentration of the bacterial suspension is preferably 1×10 7 -10 8 cfu / mL.

[0010] Preferably, in step L3, the mass volume ratio of substance 3:astaxanthin powder is 10-15:1.

[0011] The plant mixture is prepared by mixing seabuckthorn seed oil and plant extract in a volume ratio of 1:4-6, wherein the plant extract is obtained by extracting the following plants in parts by mass: 1-3 parts of hawthorn, 2-3 parts of kudzu root, 3-5 parts of gynostemma pentaphyllum, 1-2 parts of prickly ash, and 2-3 parts of white hyacinth bean.

[0012] Preferably, the preparation method of the plant extract is as follows: V1. Betaine, citric acid and water are weighed in a mass ratio of 1:1.5-1.7:18-20, heated to 50-60°C and subjected to ultrasonic treatment at a power of 100-140w, and solvent 1 is obtained after the treatment; V2. Weigh corresponding mass fractions of hawthorn, kudzu root, gynostemma pentaphyllum and white hyacinth bean, grind them into powder, sieve to obtain a plant mixed powder, weigh the plant mixed powder and the solvent 1 obtained in step V1 according to a mass-to-volume ratio of 1:15-20, heat to boiling and add the pepper, let stand for 30-45 minutes, heat to boiling again, and continue distillation for 60-80 minutes to obtain the plant extract.

[0013] Preferably, the ultrasonication in step V1 is preferably performed for 6-10 min, and the sieving in step V2 is preferably performed using a 4-10 mesh sieve.

[0014] The water-soluble complex is obtained by uniformly mixing the following materials in parts by mass: 1-2 parts of nattokinase, 3-6 parts of gum arabic, and 30-50 parts of water.

[0015] The preparation method of the contents is as follows: The solid particles are mixed with the plant mixture, stirred until uniformly dispersed, and then the water-soluble complex is added, and high-speed shearing is performed at 14000-16000 rpm for 2-3 minutes, and the contents are obtained after the end.

[0016] The capsule shell is made of common capsule shell materials, and the capsule shell is preferably prepared by containing the following materials in parts by mass: 1-2 parts of gelatin, 0.4-0.6 parts of glycerol, and 1.5-2 parts of water.

[0017] Preferably, the capsule shell may also include materials having anti-corrosion, anti-oxidation and other functions after being verified for food safety.

[0018] The present invention also provides a method for preparing a composite gel health food for lowering blood lipids and blood pressure, the specific contents of which are as follows: S1. After weighing the capsule shell material, gelatinizing, vacuumizing and keeping warm to obtain capsule shell liquid; S2. Forming a film of the capsule shell liquid obtained in step S1, filling the contents, and obtaining a composite gel health food after drying the pellets.

[0019] Preferably, the gelling in step S1 is preferably stirred at 65-75°C for 1-2h, the vacuuming is preferably performed at -0.1Mpa, and the insulation is performed at 60°C for 2-3h.

[0020] Preferably, the thickness of the capsule shell after film formation in step S2 is preferably 0.75-0.85 mm, and the drying is preferably performed at 25-30°C.

[0021] The beneficial effects of the present invention are as follows: The present invention mainly uses natural extracts to prepare a composite gel health food, wherein the content is prepared into a Pickering emulsion, and the solid particles composed of a soybean protein-porphyra polysaccharide-astaxanthin complex replace the traditional surfactant. The soybean protein is suitable for separation of two phases because it contains a large number of hydrophilic and hydrophobic groups. The polysaccharide extracted from porphyra can improve the viscosity after being added, which is conducive to enhancing the emulsification performance of the solid particles. The addition of astaxanthin gives the solid particles certain functionality, which can cooperate with the plant mixture to exert a health care effect. The plant mixture is obtained by mixing seabuckthorn seed oil with plant extracts. Seabuckthorn seed oil is more suitable for people with high blood lipids and hypertension because it contains a large number of unsaturated fatty acids. The plant extract is obtained by extracting hawthorn, kudzu root, gynostemma pentaphyllum, prickly ash and white lentil. Further, the deep eutectic solvent is used in combination with distillation for extraction. The plant extract finally obtained is used as a core functional substance to achieve the health care effect of maintaining a healthy level of blood lipids and blood pressure. In addition, prickly ash is added and soaked after boiling to alleviate the cool taste of plants such as gynostemma pentaphyllum. The water-soluble complex acts as the aqueous phase of the Pickering emulsion, specifically involving substances such as nattokinase and gum arabic. The addition of gum arabic can not only form a coating with a shielding effect outside the oil phase after the solid particles are wrapped, preventing the active substances in the oil phase from being affected by the external environment and becoming ineffective, but also promote the Pickering emulsion to form a more stable network structure. The composite gel health food is preferably prepared in the form of a capsule. The capsule shell can achieve a certain sustained release effect, which helps the composite gel health food to take effect slowly and avoid discomfort symptoms in people who take it. The capsule has the advantages of being easy to carry, clean and hygienic, and is suitable for health food applications.

[0022] The composite gel health food prepared by the present invention preferably has a capsule shell made of common soft capsule materials, such as gelatin and glycerin, and its safety has been widely verified. The contents of the composite gel health food are shown to be non-toxic after acute oral toxicity tests, three genetic toxicity tests and a 28-day oral toxicity test, and have high safety and good health care effects of maintaining blood lipids and blood pressure at healthy levels. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings: Figure 1 This is a validation diagram for blood pressure related health care effects; Figure 2 This is a diagram to verify the health effects related to blood lipids. DETAILED DESCRIPTION

[0024] In order to more clearly explain the overall concept of the present application, the following is described in detail in conjunction with the accompanying drawings of the specification by way of embodiment. In the following description, a large number of specific details are provided to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present invention, some technical features well known in the art are not described.

[0025] Example 1: This example provides a method for preparing solid particles, the specific contents are as follows: The solid particulate matter of this embodiment is a soy protein-porphyra yezoensis polysaccharide-astaxanthin complex, and the specific preparation method is as follows: L1. Weigh soy protein and water at a mass-to-volume ratio of 1:50 (g:mL), dissolve soy protein in water, stir at 150 rpm for 30 min at room temperature, store at 4°C for 10 h, adjust pH to 10.0, heat to 80°C and maintain for 20 min, and obtain substance 1; L2. Porphyra yezoensis was air-dried and ground into powder, then passed through a 60-mesh sieve, and water was added. The bacterial suspension was added. The mass ratio of Porphyra yezoensis powder: water: bacterial suspension was 1:8:0.1. The bacterial suspension was obtained by activating Lactobacillus plantarum. The Lactobacillus plantarum was from the China General Microbiological Culture Collection Center. The strain number was CGMCC No.1.573. The concentration of the bacterial suspension was 1×10 7 cfu / mL, after mixing Porphyra yezoensis powder, water and bacterial suspension, the mixture was fermented at 28°C for 24 h, and then centrifuged at 8000 g for 15 min. The supernatant was sterilized at high temperature to obtain substance 2; L3. Mix substance 1 obtained in step L1 and substance 2 obtained in step L2 in a volume ratio of 1:0.8, adjust the pH to 4.0, and continue stirring at 280 rpm for 20 min at room temperature to obtain substance 3. Weigh substance 3 and astaxanthin powder according to a mass volume ratio of 10:1 (mL:g), add astaxanthin powder to substance 3, continue stirring for 45 min, and then freeze-dry for 45 h to obtain solid particles.

[0026] Embodiment 2: This embodiment provides a method for preparing solid particles, the specific contents are as follows: The solid particulate matter of this embodiment is a soy protein-porphyra yezoensis polysaccharide-astaxanthin complex, and the specific preparation method is as follows: L1. Weigh soy protein and water at a mass-to-volume ratio of 1:55 (g:mL), dissolve soy protein in water, stir at 180 rpm for 38 min at room temperature, store at 4 °C for 11 h, adjust pH to 10.0, heat to 85 °C and maintain for 25 min, and obtain substance 1; L2. Porphyra yezoensis was air-dried and ground into powder, then passed through a 70-mesh sieve, and water was added. The bacterial suspension was added. The mass ratio of Porphyra yezoensis powder: water: bacterial suspension was 1:9:0.2. The bacterial suspension was obtained after activation of Lactobacillus plantarum. Lactobacillus plantarum was from the China General Microbiological Culture Collection Center. The strain number was CGMCC No.1.573. The concentration of the bacterial suspension was 5×10 7 cfu / mL, after mixing Porphyra yezoensis powder, water and bacterial suspension, the mixture was fermented at 29°C for 25 h, and then centrifuged at 9000 g for 18 min. The supernatant was sterilized at high temperature to obtain substance 2; L3. Mix substance 1 obtained in step L1 and substance 2 obtained in step L2 in a volume ratio of 1:1.2, adjust the pH to 4.0, and continue stirring at 300 rpm for 24 min at room temperature to obtain substance 3. Weigh substance 3 and astaxanthin powder according to a mass volume ratio of 12:1 (mL:g), add astaxanthin powder to substance 3, continue stirring for 50 min, and then freeze-dry for 48 h to obtain solid particles.

[0027] Embodiment 3: This embodiment provides a method for preparing solid particles, the specific contents are as follows: The solid particulate matter of this embodiment is a soy protein-porphyra yezoensis polysaccharide-astaxanthin complex, and the specific preparation method is as follows: L1. Weigh soy protein and water at a mass-to-volume ratio of 1:60 (g:mL), dissolve soy protein in water, stir at 200 rpm for 45 min at room temperature, store at 4°C for 12 h, adjust pH to 10.0, heat to 90°C and maintain for 30 min, and obtain substance 1; L2. After drying, grind the Porphyra yezoensis into powder and pass it through an 80-mesh sieve, add water, and add the bacterial suspension. The mass ratio of Porphyra yezoensis powder: water: bacterial suspension is 1:10:0.3. The bacterial suspension is obtained after activation of Lactobacillus plantarum. The Lactobacillus plantarum comes from the China General Microbiological Culture Collection Center. The strain number is CGMCC No.1.573. The concentration of the bacterial suspension is 1×10 8 cfu / mL, after mixing Porphyra yezoensis powder, water and bacterial suspension, the mixture was fermented at 30°C for 28 h, and then centrifuged at 10000 g for 20 min. The supernatant was sterilized at high temperature to obtain substance 2; L3. Mix substance 1 obtained in step L1 and substance 2 obtained in step L2 in a volume ratio of 1:1.5, adjust the pH to 4.0, and continue stirring at 320 rpm for 30 min at room temperature to obtain substance 3. Weigh substance 3 and astaxanthin powder according to a mass volume ratio of 15:1 (mL:g), add astaxanthin powder to substance 3, continue stirring for 60 min, and then freeze-dry for 50 h to obtain solid particles.

[0028] Embodiment 4: This embodiment provides a method for preparing a plant mixture, the specific contents are as follows: The plant mixture of this embodiment is composed of seabuckthorn seed oil and plant extracts, wherein the plant extracts are obtained by extracting the following plants in parts by weight: 1 part of hawthorn, 2 parts of kudzu root, 3 parts of gynostemma pentaphyllum, 1 part of Zanthoxylum bungeanum, and 2 parts of white hyacinth bean; The preparation method of plant extract is as follows: V1. Betaine, citric acid and water were weighed in a mass ratio of 1:1.5:18, heated to 50°C and subjected to ultrasonic treatment at a power of 100 W for 6 min, and solvent 1 was obtained after the treatment; V2. Weigh the corresponding mass fractions of hawthorn, kudzu root, gynostemma pentaphyllum and white hyacinth bean, grind them into powder, pass through a 4-mesh sieve to obtain a plant mixed powder, weigh the plant mixed powder and the solvent 1 obtained in step V1 according to a mass volume ratio of 1:15, heat to boiling and add the pepper, let stand for 30 minutes, heat to boiling again, and continue distillation for 60 minutes to obtain a plant extract; The seabuckthorn seed oil and the plant extract obtained above were uniformly mixed in a volume ratio of 1:4 to obtain the plant mixture of this embodiment.

[0029] Embodiment 5: This embodiment provides a method for preparing a plant mixture, the specific contents are as follows: The plant mixture of this embodiment is composed of seabuckthorn seed oil and plant extracts, wherein the plant extracts are obtained by extracting the following plants in parts by weight: 2 parts of hawthorn, 2.4 parts of kudzu root, 4 parts of gynostemma pentaphyllum, 1.5 parts of prickly ash, and 2.5 parts of white hyacinth bean; The preparation method of plant extract is as follows: V1. Betaine, citric acid and water were weighed in a mass ratio of 1:1.6:19, heated to 55°C and subjected to ultrasonic treatment at a power of 120W for 8 minutes, and solvent 1 was obtained after the treatment; V2. Weigh the corresponding mass fractions of hawthorn, kudzu root, gynostemma pentaphyllum and white hyacinth bean, grind them into powder, pass through an 8-mesh sieve to obtain a plant mixed powder, weigh the plant mixed powder and the solvent 1 obtained in step V1 according to a mass volume ratio of 1:18, heat to boiling and add the pepper, let stand for 38 minutes, heat to boiling again, and continue distillation for 70 minutes to obtain a plant extract; The seabuckthorn seed oil and the plant extract obtained above were uniformly mixed in a volume ratio of 1:5 to obtain the plant mixture of this embodiment.

[0030] Embodiment 6: This embodiment provides a method for preparing a plant mixture, the specific contents are as follows: The plant mixture of this embodiment is composed of seabuckthorn seed oil and plant extracts, wherein the plant extracts are obtained by extracting the following plants in parts by weight: 3 parts of hawthorn, 3 parts of kudzu root, 5 parts of gynostemma pentaphyllum, 2 parts of prickly ash, and 3 parts of white hyacinth bean; The preparation method of plant extract is as follows: V1. Betaine, citric acid and water were weighed in a mass ratio of 1:1.7:20, heated to 60°C and subjected to ultrasonic treatment at a power of 140W for 10 min, and solvent 1 was obtained after the treatment; V2. Weigh the corresponding mass fractions of hawthorn, kudzu root, gynostemma pentaphyllum and white hyacinth bean, grind them into powder, pass through a 10-mesh sieve to obtain a plant mixed powder, weigh the plant mixed powder and the solvent 1 obtained in step V1 according to a mass volume ratio of 1:20, heat to boiling and add the pepper, let stand for 45 minutes, heat to boiling again, and continue distillation for 80 minutes to obtain a plant extract; The seabuckthorn seed oil and the plant extract obtained above were uniformly mixed in a volume ratio of 1:6 to obtain the plant mixture of this embodiment.

[0031] Example 7: This example provides a method for preparing the contents of a composite gel health food for lowering blood lipids and blood pressure, the specific contents are as follows: The contents of this example include the following raw materials in parts by weight: 3 parts of solid particles, 8 parts of plant mixture, and 50 parts of water-soluble complex, wherein the solid particles are prepared from Example 1, and the plant mixture is prepared from Example 4; The water-soluble complex is obtained by uniformly mixing the following materials in parts by weight: 1 part of nattokinase, 3 parts of gum arabic, and 30 parts of water; The solid particles are mixed with the plant mixture, stirred until uniformly dispersed, and then the water-soluble complex is added and high-speed sheared at 14000 rpm for 3 minutes. After the high-speed shearing, the contents of the composite gel health food for lowering blood lipids and blood pressure are obtained.

[0032] Example 8: This example provides a method for preparing the contents of a composite gel health food for lowering blood lipids and blood pressure, the specific contents are as follows: The contents of this example include the following raw materials in parts by weight: 4 parts of solid particles, 9 parts of plant mixture, and 55 parts of water-soluble complex, wherein the solid particles are prepared from Example 2, and the plant mixture is prepared from Example 5; The water-soluble complex is obtained by uniformly mixing the following materials in parts by weight: 1.5 parts of nattokinase, 5 parts of gum arabic, and 40 parts of water; The solid particles are mixed with the plant mixture, stirred until uniformly dispersed, and then the water-soluble complex is added, and high-speed shearing is performed at 15000 rpm for 2.5 minutes. After the end, the content of the composite gel health food for lowering blood lipids and blood pressure is obtained.

[0033] Example 9: This example provides a method for preparing the contents of a composite gel health food for lowering blood lipids and blood pressure, the specific contents are as follows: The contents of this example include the following raw materials in parts by weight: 5 parts of solid particles, 12 parts of plant mixture, and 60 parts of water-soluble complex, wherein the solid particles are prepared from Example 3, and the plant mixture is prepared from Example 6; The water-soluble complex is obtained by uniformly mixing the following materials in parts by weight: 2 parts of nattokinase, 6 parts of gum arabic, and 50 parts of water; The solid particles are mixed with the plant mixture, stirred until uniformly dispersed, and then the water-soluble complex is added and high-speed sheared at 16000 rpm for 2 minutes. After the end, the content of the composite gel health food for lowering blood lipids and blood pressure is obtained.

[0034] Embodiment 10: This embodiment provides a method for preparing a composite gel health food for lowering blood lipids and blood pressure, and the specific contents are as follows: The composite gel health food for lowering blood lipids and blood pressure of this embodiment is composed of two parts: the content and the capsule shell; The contents are obtained from Example 7, and the capsule shell is prepared from the following materials in parts by weight: 1 part gelatin, 0.4 part glycerol, and 1.5 parts water; S1. The capsule shell material was weighed and stirred at 65°C for 1 h to achieve gelation, and then vacuumed at -0.1 Mpa and kept at 60°C for 2 h to obtain the capsule shell liquid; S2. Form the shell liquid obtained in step S1 into a film with a thickness of 0.75 mm, fill the contents, and obtain a pellet, which is dried at 25° C. for 12 hours to obtain a composite gel health food.

[0035] Example 11: This example provides a method for preparing a composite gel health food for lowering blood lipids and blood pressure, the specific contents are as follows: The composite gel health food for lowering blood lipids and blood pressure of this embodiment is composed of two parts: the content and the capsule shell; The contents are obtained from Example 8, and the capsule shell is prepared from the following materials in parts by weight: 1.5 parts of gelatin, 0.5 parts of glycerol, and 1.8 parts of water; S1. The capsule shell material was weighed and stirred at 70°C for 1.5h to achieve gelation, and then vacuumed at -0.1Mpa and kept at 60°C for 2.5h to obtain the capsule shell liquid; S2. Form the shell liquid obtained in step S1 into a film with a thickness of 0.8 mm, fill the contents, and obtain a pellet, which is dried at 28° C. for 12 hours to obtain a composite gel health food.

[0036] Example 12: This example provides a method for preparing a composite gel health food for lowering blood lipids and blood pressure, the specific contents are as follows: The composite gel health food for lowering blood lipids and blood pressure of this embodiment is composed of two parts: the content and the capsule shell; The contents are obtained from Example 9, and the capsule shell is prepared from the following materials in parts by weight: 2 parts gelatin, 0.6 parts glycerol, and 2 parts water; S1. The capsule shell material was weighed and stirred at 75°C for 2h to achieve gelation, and then vacuumed at -0.1Mpa and kept at 60°C for 3h to obtain capsule shell liquid; S2. Form the shell liquid obtained in step S1 into a film with a thickness of 0.85 mm, fill the contents, and obtain a pellet, which is dried at 30° C. for 12 hours to obtain a composite gel health food.

[0037] The filling volume of the contents in the composite gel health foods obtained in Examples 10-12 was 1.5 mL.

[0038] Experimental test: According to the relevant regulations on health food, the composite gel health food obtained by the present invention was subjected to toxicological tests, specifically involving acute oral toxicity tests, three genetic toxicity tests and a 28-day oral toxicity test. Some of the test results are recorded as follows: 1. Acute oral toxicity test: SPF grade SD healthy rats, half male and half female, weighing 180-200 g, were selected. They were fasted but not watered for 16 h before the start of the experiment. The contents of the composite gel health food obtained in Example 7, Example 8, and Example 9 were gavaged at 20 g / kg three times a day, with an interval of 4 h each time, for 14 days. After the end, the poisoning symptoms and death of the rats were recorded.

[0039] Table 1 Results of acute oral toxicity test

[0040] The results of the acute oral toxicity test on rats are shown in Table 1. As can be seen from Table 1, according to the maximum tolerated dose method (500 times the recommended dose is used here), when the animals do not die, it means that the tested samples can enter the next stage of toxicology testing. The contents of the composite gel health foods obtained in Examples 7-9 are all highly safe, and the materials involved in the capsule shell of the composite gel health food have been widely verified for their safety. Therefore, the composite gel health food passed the acute oral toxicity test and is non-toxic.

[0041] 2. Three genetic toxicity tests: The three genetic toxicity tests are the Ames test, the mouse bone marrow micronucleus test, and the mouse sperm abnormality test.

[0042] 1) Ames test: Four strains of Salmonella typhimurium mutants TA97, TA98, TA100, and TA102 were used for the test. The plate incorporation method was used. The activation system was a mixture of rat liver S9 induced by polychlorinated biphenyls. The positive substances were dichlorvos (TA97, TA98, and TA102) and sodium azide (TA100) for the -S9, 2-aminofluorene (TA97, TA98, and TA100) and 1,8-hydroxyanthraquinone (TA102) for the +S9, and a negative control (distilled water) and a solvent control (sterile dimethyl sulfoxide) were set up. The test was carried out with or without the addition of S9 mixture at a dose of 2μg / (100μL·dish), 40μg / (100μL·dish), 200μg / (100μL·dish), 1000μg / (100μL·dish), and 5000μg / (100μL·dish). Three parallel groups were set up for any concentration. The contents of the composite gel health food obtained in Examples 7-9 were all subjected to Ames test. The test results of the contents of the composite gel health food obtained in Example 7 are listed below.

[0043] Table 2 Ames test results (first time)

[0044] Table 3 Ames test results (second time)

[0045] The results of the Ames test are shown in Tables 2 and 3. It can be seen from Tables 2 and 3 that the contents of the composite gel health food obtained in Example 7 have no mutagenic effect on the mutants of Salmonella typhimurium TA97, TA98, TA100, and TA102 at the test dose. Similarly, since the safety of the capsule shell has been widely verified, it can be proved here that the composite gel health food obtained by the present invention has no mutagenic effect.

[0046] 2) Mouse bone marrow micronucleus test: The contents of the composite gel health food obtained in Examples 7-9 were used for the test. Taking the contents of the composite gel health food obtained in Example 7 as an example, 50 ICR-level mice weighing 25-30g were selected and randomly divided into 5 groups, with 10 mice in each group, half of which were male and half were female. The doses of 10g / (kg·bw), 5g / (kg·bw), and 2.5g / (kg·bw) were set for the test, cyclophosphamide (0.04g / (kg·bw)) was used as the positive control, and distilled water was used as the negative control. 0.1mL / 10g of mouse weight was used for gavage, and the double dosing method was adopted. The animals were killed 6 hours after the last administration, and the sternum was taken for routine preparation and microscopic examination. 1000 bone marrow polychromatic erythrocytes (PCE) were counted for each mouse, the PCE containing micronuclei were calculated, and the micronucleus rate was calculated.

[0047] Table 4 Results of mouse bone marrow micronucleus test

[0048] The results of the mouse bone marrow micronucleus test are shown in Table 4. It can be seen from Table 4 that the contents of the composite gel health food obtained in Examples 7-9 did not affect the micronuclei of mouse bone marrow polychromatic erythrocytes. Similarly, since the safety of the capsule shell has been widely verified, it can be proved here that the composite gel health food obtained by the present invention has no effect on the micronuclei of mouse bone marrow polychromatic erythrocytes.

[0049] 3) Mouse sperm deformity test: The contents of the composite gel health food obtained in Examples 7-9 were used for the test. Taking the contents of the composite gel health food obtained in Example 7 as an example, 25 ICR-grade sexually mature male mice with a body weight of 30-35g were selected and randomly divided into 5 groups, with 5 mice in each group. The doses of 10g / (kg·bw), 5g / (kg·bw), and 2.5g / (kg·bw) were set for the test. Cyclophosphamide (0g / (kg·bw)) was used as a positive control, and distilled water was used as a negative control. 0.1mL / 10g of mouse weight was gavaged once a day at the same time for five consecutive days. The mice were killed 30 days after the last gavage. The epididymis on both sides was taken for routine preparation and microscopic examination. 1000 sperm with complete structure were counted for each mouse, and the sperm deformity rate was calculated.

[0050] Table 5 Results of mouse sperm abnormality test

[0051] The results of the mouse sperm deformity test are shown in Table 5. It can be seen from Table 5 that the contents of the composite gel health foods obtained in Examples 7-9 will not cause mouse sperm deformity. Similarly, since the safety of the capsule shell has been widely verified, it can be proved here that the composite gel health food obtained by the present invention will also not cause mouse deformity.

[0052] The 28-day oral toxicity test was also conducted in accordance with the relevant standards for toxicological testing of health foods. After 28 days of feeding, the autopsy results showed that the rats' organs developed normally and had no lesions.

[0053] 3. Verification of the effect of health food: The composite gel health food obtained by the present invention claims to have the health effects of lowering blood lipids and blood pressure. The preparation process does not involve the addition of drugs with clear therapeutic effects. The health effects that can be achieved come from various natural extracts. According to the records of the Health Food Function Test and Evaluation Method (2023 Edition), the effects of the contents of the composite gel health food obtained in the example on maintaining healthy levels of blood lipids and blood pressure are verified.

[0054] 1) Verification of blood pressure related health effects: According to the records of the above documents, a renal vascular hypertension rat model was prepared, and SPF SD rats were used for the experiment. A model group, a control group and an experimental group were prepared. The model group was not gavaged after modeling, and the control group was gavaged with normal saline. The experimental group involved three doses: 2g / kg, 1g / kg, and 0.5g / kg. The experimental samples were specifically the contents of the composite gel health food obtained in Examples 7-9. The experiment was carried out for a total of 30 days, during which the tail pulse pressure was measured 1-2 times a week.

[0055] Figure 1 The effect of the contents of the composite gel health food obtained in Example 8 on the blood pressure of SD rats is demonstrated. Compared with the control group, the blood pressure of the rats in the three experimental groups decreased to varying degrees. When used at a dose of 2 g / kg, the blood pressure decreased significantly. However, it is usually recommended to use it at a low dose to achieve a safer and more soothing effect of regulating blood sugar balance.

[0056] 2) Verification of blood lipid related health effects: SPF SD rats were also used to construct a mixed hyperlipidemia model. The experimental dose was the same as above. The experiment was carried out for 30 days, and the concentrations of TC, TG, LDL-C, and HDL-C in serum were detected after the experiment.

[0057] The results are as follows Figure 2 As shown by Figure 2It can be seen that compared with the control group, the contents of the composite gel health food obtained in Examples 7-9 have a significant effect of reducing triglycerides and cholesterol, while high-density lipoprotein cholesterol is increased, proving that the contents of the composite gel health food obtained in Examples 7-9 are helpful in maintaining healthy blood lipid levels.

[0058] In addition, the contents of the composite gel health food obtained in Examples 7-9 were verified for their blood sugar lowering effects. Animal experiments focused on fasting blood sugar and glucose tolerance indicators. Individual results were significant, but they cannot be fully described as the contents also having the effect of maintaining blood sugar balance.

[0059] The above are only several embodiments of the present invention and are not intended to limit the present invention in any form. Although the present invention is disclosed as above in the form of a preferred embodiment, it is not intended to limit the present invention. Any technician familiar with the profession, without departing from the scope of the technical solution of the present invention, using the technical content disclosed above to make slight changes or modifications are equivalent to equivalent implementation cases and fall within the protection scope of the technical solution of the present invention.

Claims

1. A composite gel health food for lowering blood lipids and blood pressure, characterized in that: The composite gel health food is composed of two parts: the content and the capsule shell; The contents include the following raw materials in parts by mass: 3-5 parts of solid particles, 8-12 parts of plant mixture, and 50-60 parts of water-soluble complex; The solid particles are a soybean protein-porphyra yezoensis polysaccharide-astaxanthin complex, and the specific preparation method is as follows: L1. Dissolve soy protein in water and continue stirring at room temperature. After storage at 4°C, adjust the pH to 10.0 and heat to obtain substance 1. L2. The dried Porphyra yezoensis was ground into powder and then sieved, water was added, the bacterial suspension was added, fermented, and then centrifuged at high speed. The supernatant was sterilized at high temperature to obtain substance 2; L3. The substance 1 obtained in step L1 and the substance 2 obtained in step L2 are mixed in a volume ratio of 1:0.8-1.5, the pH is adjusted to 4.0, and stirring is continued at room temperature to obtain substance 3, and astaxanthin powder is added, stirring is continued, and after completion, freeze-drying is performed to obtain solid particles; In step L2, the mass ratio of Porphyra yezoensis powder: water: bacterial suspension is 1: 8-10: 0.1-0.3; In step L3, the mass volume ratio of substance 3: astaxanthin powder is 10-15:1; The plant mixture is prepared by mixing seabuckthorn seed oil and plant extract in a volume ratio of 1:4-6. The plant extract is obtained by extracting the following plants in parts by mass: 1-3 parts of hawthorn, 2-3 parts of kudzu root, 3-5 parts of gynostemma pentaphyllum, 1-2 parts of prickly ash, and 2-3 parts of white hyacinth bean.

2. The composite gel health food for lowering blood lipids and blood pressure according to claim 1, characterized in that: The mass volume ratio of soybean protein to water in step L1 is 1:50-60, and the bacterial suspension in step L2 is obtained after activation of Lactobacillus plantarum.

3. The composite gel health food for lowering blood lipids and blood pressure according to claim 2, characterized in that: The preparation method of the plant extract is as follows: V1. Betaine, citric acid and water are weighed in a mass ratio of 1:1.5-1.7:18-20, and heated and ultrasonicated to obtain solvent 1; V2. Weigh corresponding mass portions of hawthorn, kudzu root, gynostemma pentaphyllum and white hyacinth bean, grind them into powder, sieve to obtain a plant mixed powder, weigh the plant mixed powder and the solvent 1 obtained in step V1 according to a mass-to-volume ratio of 1:15-20, heat to boiling and then add the pepper, let it stand, heat to boiling again, and obtain the plant extract after continuous distillation.

4. The composite gel health food for lowering blood lipids and blood pressure according to claim 3, characterized in that: The water-soluble complex is obtained by uniformly mixing the following materials in parts by mass: 1-2 parts of nattokinase, 3-6 parts of gum arabic, and 30-50 parts of water.

5. The composite gel health food for lowering blood lipids and blood pressure according to claim 4, characterized in that: The preparation method of the contents is as follows: The solid particles are mixed with the plant mixture, stirred until uniformly dispersed, and then the water-soluble complex is added and sheared at high speed to obtain the contents.

6. The composite gel health food for lowering blood lipids and blood pressure according to claim 5, characterized in that: The capsule shell contains the following materials in parts by weight: 1-2 parts of gelatin, 0.4-0.6 parts of glycerol, and 1.5-2 parts of water.

7. A method for preparing the composite gel health food for lowering blood lipids and blood pressure according to any one of claims 1 to 6, characterized in that: The specific preparation steps are as follows: S1. After weighing the capsule shell material, gelatinizing, vacuumizing and keeping warm to obtain capsule shell liquid; S2. Forming a film of the capsule shell liquid obtained in step S1, filling the contents, and obtaining a composite gel health food after drying the pellets.

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