Autoserum eye drops fused with nano-copper material as well as preparation method and application of autoserum eye drops

Abstract

The invention relates to an autoserum eye drop fused with a nano-copper material and a preparation method and application thereof, and the autoserum eye drop is prepared by matching a prepared copper sulfate compound solution with an autoserum extract. The autoserum eye drops can be used as a substitute of tears and have the effects of lubricating, promoting ocular surface epithelium repair and improving ocular surface microenvironment, the nano-copper component of the copper sulfate compound solution fused in the autoserum eye drops can effectively resist and kill bacteria, and on the basis of not additionally adding other preservatives, the autoserum eye drops have the advantages of being safe and reliable. The preservation time of the autoserum eye drops is effectively prolonged.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to an autologous serum eye drop solution incorporating nano-copper materials, and a preparation method and application thereof. Background Art

[0002] In order to extend the effective time of existing eye drops, a large amount of preservatives are often added to the eye drops to increase the shelf life of the eye drops. However, a large amount of preservatives are often harmful to the human body. Therefore, how to increase the bactericidal function of eye drops without using preservatives, thereby increasing the shelf life of eye drops and reducing toxic side effects on the human body is a technical problem that needs to be solved urgently. Summary of the invention

[0003] In order to solve the shortcomings of the prior art, the present invention provides an autologous serum eye drop incorporating nano-copper material and a preparation method and application thereof.

[0004] The technical solution adopted by the present invention is: an autologous serum eye drop solution incorporating nano-copper material, wherein the eye drop solution is composed of a copper sulfate compound solution with a mass fraction of 15% and an autologous serum extract eye drop solution with a mass fraction of 85%.

[0005] Specifically, the copper sulfate compound solution is prepared from copper sulfate, PEG6000, and the remainder is deionized water.

[0006] Specifically, the copper sulfate is food and pharmaceutical grade copper sulfate.

[0007] The copper sulfate composite solution is prepared by the following steps: (1) Heat PEG6000 to 100℃ until it is completely melted, then cool it to 60℃ and keep it constant to ensure that the solid melts into the liquid. Stir at 50 rpm. After the solid is completely melted, stir at 120 rpm. Stir for 15 minutes after melting, and cool down while stirring.

[0008] (2) Add copper sulfate to deionized water, heat it, and introduce it into the PEG6000 solution and stir for 15 minutes to form a copper sulfate complex solution.

[0009] Specifically, the heating temperature in step (2) is 60°C.

[0010] Specifically, the mass ratio of copper sulfate to PEG6000 in the copper sulfate composite solution is 1:1-2.

[0011] Specifically, the mass ratio of copper sulfate to PEG6000 in the copper sulfate composite solution is 1:1.

[0012] A method for preparing autologous serum eye drops incorporating nano-copper materials comprises the following steps: (1) Clean the beaker with alcohol, disinfect the ultrasonic head of the homogenizer, and clean it with pure water; (2) Pour the copper sulfate compound solution and the autologous serum extract eye drops solution into a beaker, and stir them using a disperser homogenizer and an ultrasonic stirrer to obtain the autologous serum eye drops incorporating the nano-copper material.

[0013] The stirring time in step (2) is 20-40 minutes.

[0014] Application of autologous serum eye drops incorporating nano-copper materials in the preparation of long-acting antibacterial drugs.

[0015] The beneficial effects of the present invention are as follows: the present invention provides an autologous serum eye drops incorporating nano-copper materials, and a preparation method and application thereof, wherein the prepared copper sulfate compound solution is combined with an autologous serum extract to obtain an autologous serum eye drop that can be used as a substitute for tears, has the functions of lubricating, promoting ocular surface epithelial repair, and improving the ocular surface microenvironment, wherein the nano-copper component of the copper sulfate compound solution incorporated therein can effectively resist bacteria and sterilize, and effectively increases the shelf life of the autologous serum eye drops without adding other preservatives. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] Example 1: Preparation of copper sulfate composite solution Jinghe Nano: Food and drug grade copper sulfate 10% Dow: PEG6000: 10% Deionized water: 80% Process: (1) Take 10g of Dow PEG6000, heat it to 100℃ until it is completely melted, then cool it to 60℃ and keep it constant to ensure that the solid melts into the liquid. At the beginning, the stirrer speed is 50 rpm. After the solid is completely melted, the stirrer speed is 120 rpm). Stir for 15 minutes after melting, while cooling down.

[0018] (2) Take 80 g of deionized water, add 10 g of copper sulfate, heat to 60°C, introduce into the PEG6000 solution and stir for 15 minutes to form a copper sulfate complex solution.

[0019] Example 2: Preparation of copper sulfate composite solution Jinghe Nano: Food and drug grade copper sulfate 10% Dow: PEG6000: 20% Deionized water: 70% Process: (1) Take 20g of Dow PEG6000, heat it to 100℃ until it is completely melted, then cool it to 60℃ and keep it constant to ensure that the solid melts into the liquid. At the beginning, the stirrer speed is 50 rpm. After the solid is completely melted, the stirrer speed is 120 rpm). Stir for 15 minutes after melting, while cooling down while stirring.

[0020] (2) Take 70 g of deionized water, add 10 g of copper sulfate, heat to 60°C, introduce into the PEG6000 solution and stir for 15 minutes to form a copper sulfate complex solution.

[0021] Example 3: Preparation process of copper sulfate solution and autologous serum eye drops Copper sulfate compound solution: 15% Autologous serum extract eye drops: 85% Process: (1) Clean the beaker with alcohol, disinfect the ultrasonic head of the Holder HD-CP150 homogenizer, and clean it with pure water; (2) Take 15 g of the copper sulfate compound solution prepared in Example 1 and 85 g of the autologous serum extract eye drops solution; (3) Pour the two ingredients into a beaker and stir for 30 minutes using a Holder HD-CP150 homogenizer ultrasonic stirrer to obtain autologous serum eye drops containing nano-copper material.

[0022] Example 4: Preparation process of copper sulfate solution and autologous serum eye drops Copper sulfate compound solution: 15% Autologous serum extract eye drops: 85% Process: (1) Clean the beaker with alcohol, disinfect the ultrasonic head of the Holder HD-CP150 homogenizer, and clean it with pure water; (2) Take 15 g of the copper sulfate compound solution prepared in Example 2 and 85 g of the autologous serum extract eye drops solution; (3) Pour the two ingredients into a beaker and stir for 30 minutes using a Holder HD-CP150 homogenizer ultrasonic stirrer to obtain autologous serum eye drops containing nano-copper material.

[0023] Comparative Example 5: Autologous serum extract eye drops + deionized water Process: (1) Clean the beaker with alcohol, disinfect the ultrasonic head of the Holder HD-CP150 homogenizer, and clean it with pure water; (2) Take 15 g of deionized water and 85 g of autologous serum extract eye drops solution; (3) Pour the two materials into a beaker and stir for 30 minutes using a Holder HD-CP150 homogenizer ultrasonic stirrer.

[0024] The eye drops prepared in Examples 3-4 and Comparative Example 5 were respectively tested in an exposed environment to simulate the situation after opening, and the obtained data are recorded in the following table:

[0025] By comparing the relevant data in the analysis table, it can be seen that the autologous serum eye drops prepared by the present invention and incorporating nano-copper materials can effectively play an antibacterial and bactericidal role, and effectively increase the shelf life of the autologous serum eye drops without adding other preservatives.

[0026] The above are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should be regarded as the protection scope of the present invention.

[0027] Technical personnel should note that although the present invention has been described according to the above specific implementation methods, the inventive concept of the present invention is not limited to this invention, and any modification using the inventive concept will be included in the scope of protection of this patent.

Claims

1. An autologous serum eye drop incorporating nano-copper material, characterized in that: The eye drops are composed of 15% by mass copper sulfate compound solution and 85% by mass autologous serum extract eye drops.

2. The autologous serum eye drops incorporating nano-copper materials according to claim 1, characterized in that: The copper sulfate compound solution is prepared from copper sulfate, PEG6000, and the balance is deionized water.

3. The autologous serum eye drops incorporating nano-copper materials according to claim 2, characterized in that: The copper sulfate is food and drug grade copper sulfate.

4. The autologous serum eye drops incorporating nano-copper materials according to claim 2, characterized in that: The copper sulfate composite solution is prepared by the following steps: (1) Heat PEG6000 to 100°C until it is completely melted, then cool it down to 60°C and keep it constant to ensure that the solid melts into the liquid. Stir at 50 rpm. After the solid is completely melted, stir at 120 rpm. Stir for 15 minutes after melting, and cool down while stirring. (2) Add copper sulfate to deionized water, heat it, and introduce it into the PEG6000 solution and stir for 15 minutes to form a copper sulfate complex solution.

5. The autologous serum eye drops incorporating nano-copper materials according to claim 4, characterized in that: The heating temperature in step (2) is 60°C.

6. The autologous serum eye drops incorporating nano-copper materials according to claim 2, characterized in that: The mass ratio of copper sulfate to PEG6000 in the copper sulfate composite solution is 1:1-2.

7. The autologous serum eye drops incorporating nano-copper material according to claim 6, characterized in that: The mass ratio of copper sulfate to PEG6000 in the copper sulfate composite solution is 1:

1.

8. A method for preparing the autologous serum eye drops incorporating nano-copper materials according to claim 1, characterized in that: The following steps are involved: (1) Clean the beaker with alcohol, disinfect the ultrasonic head of the homogenizer, and clean it with pure water; (2) Pour the copper sulfate compound solution and the autologous serum extract eye drops solution into a beaker, and stir them using a disperser homogenizer and an ultrasonic stirrer to obtain the autologous serum eye drops incorporating the nano-copper material.

9. The preparation method according to claim 8, characterized in that: The stirring time in step (2) is 20-40 minutes.

10. Use of the autologous serum eye drops incorporating nano-copper materials according to claim 1 in the preparation of long-acting antibacterial drugs.

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