Nutrient solution and method for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia

Abstract

The invention relates to the technical field of Chinese herbal medicine planting, in particular to a nutrient solution and method for promoting the rapid growth of sagittaria tissue culture seedlings; the nutrient solution comprises: A and B composed of pine root suspensions of different concentrations, NAA and IBA, and a nutrient solution C composed of sheep manure fermentation liquid, humic acid, water-soluble compound fertilizer and potassium dihydrogen phosphate. The invention creatively adopts a suspension prepared from pine root systems, adds NAA and IBA of a certain concentration, soaks at the beginning of cultivation, and regularly waters after cultivation, which can rapidly promote the growth of sagittaria root systems, is beneficial to the absorption of water by sagittaria, can greatly improve the survival rate of sagittaria domestication, and at the same time, the growth of the root system is beneficial to the intake of nutrients, and in the middle and late stages, a nutrient solution composed of sheep manure fermentation liquid, humic acid, water-soluble compound fertilizer and potassium dihydrogen phosphate is used to promote the rapid expansion and growth of sagittaria pseudobulbs in a short period of time and become commercial seedlings.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese herbal medicine planting, and in particular to a nutrient solution and a method for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia. Background Art

[0002] Mao Ci Mushroom is the name of the medicinal material of the azalea orchid. It has the effects of clearing away heat and detoxifying, resolving phlegm and dispersing stagnation. It is mainly used to treat carbuncle, furuncle, scrofula, phlegm, snake and insect bites, lameness, lumps and lumps. It has the effects of anti-malignant tumors such as liver cancer, lung cancer, breast cancer, anti-hypertensive effects, anti-bacterial effects, antioxidant effects, anti-ventilation effects, lipid-lowering and anti-atherosclerosis effects, etc. Mao Ci Mushroom is clinically used for the treatment of internal, external, gynecological, pediatric, otorhinolaryngology, skin and other diseases, and its indications are constantly expanding. In particular, Mao Ci Mushroom is used clinically as a compound medicine for anti-tumor treatment and has achieved good results.

[0003] The hairy arrowhead is basically unable to reproduce sexually under natural conditions, and the symbiotic bacteria isolated from it promote germination, which has a certain promoting effect. The invention patent with publication number: CN100362907C discloses a biotechnology method for in vitro culture and rapid propagation of azalea orchid, the invention patent with publication number: CN1623373A discloses a method for tissue culture and rapid propagation of azalea orchid, the invention patent with publication number: CN109105264A discloses a method for rapid cultivation of regenerated plants of azalea orchid, and the invention patent with publication number: CN10 The invention patent of 9699498A discloses a method for cultivating callus tissue of azalea orchid, the invention patent of publication number: CN110100733A discloses a method for rapidly inducing regeneration of azalea orchid plants by using azalea orchid tissue culture roots as explants, the invention patent of publication number: CN113615576A discloses a method for increasing the proliferation coefficient by increasing joints and jointing of azalea orchid pseudobulbs, and the invention patent of publication number: CN112655555A discloses a method for improving the survival rate of azalea orchid tissue culture seedlings. The above methods achieve rapid propagation of Sagittaria pubescens, but all of them have problems such as low survival rate and slow growth rate after transplantation of tissue culture seedlings. Summary of the invention

[0004] The purpose of the present invention is to provide a nutrient solution and method for promoting the rapid growth of Cnidium monnieri tissue culture seedlings in response to the problems existing in the background technology.

[0005] To achieve the above object, the technical solution adopted by the present invention is: a nutrient solution for promoting the rapid growth of tissue culture seedlings of Radix Sagittifolia, comprising: a nutrient solution A for soaking tissue culture seedlings or irrigating roots during tissue culture seedling acclimation and transplantation, a nutrient solution B for irrigating roots 7 to 10 days after tissue culture seedling acclimation and transplantation, and a nutrient solution C for irrigating roots 20 to 25 days after tissue culture seedling acclimation and transplantation;

[0006] The formula of the nutrient solution A is: pine root suspension 4.0-5.0 ml / L + NAA 5.0-6.0 mg / L + IBA 1.0-2.0 mg / L;

[0007] The formula of the nutrient solution B is: pine root suspension 10.0-12.0 ml / L + NAA 30.0-35.0 mg / L + IBA 10.0-15.0 mg / L;

[0008] The formula of the nutrient solution C is: 10.0-20.0 ml / L of sheep manure fermentation liquid + 0.6-0.8 g / L of humic acid + 1.0-1.2 g / L of water-soluble compound fertilizer + 0.5-0.8 g / L of potassium dihydrogen phosphate.

[0009] Further:

[0010] The formula of the nutrient solution A is: pine root suspension 4.5 ml / L + NAA 5.5 mg / L + IBA 1.1 mg / L;

[0011] The formula of the nutrient solution B is: pine root suspension 11.0 ml / L + NAA 32.0 mg / L + IBA 13.0 mg / L;

[0012] The formula of the nutrient solution C is: sheep manure fermentation liquid 11.0 ml / L + humic acid 0.7 g / L + water-soluble compound fertilizer 1.1 g / L + potassium dihydrogen phosphate 0.6 g / L.

[0013] Furthermore, the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:20:20.

[0014] Furthermore, the preparation method of the pine root suspension is: take 2 to 4 g of pine root, add 1 L of water, and squeeze it with a soybean milk machine at room temperature for 5 to 10 minutes.

[0015] Furthermore, the preparation method of the sheep manure fermentation liquid is: take 100-200g of the fermented sheep manure, add 1L of water, store it in a sealed container for 1 month, heat it to boiling, and then cool it to room temperature.

[0016] The second object of the present invention is to provide a method for promoting the rapid growth of Pleurotus eryngii tissue culture seedlings using a nutrient solution, the steps comprising:

[0017] (1) Transplanting the tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation into a mixed substrate and acclimatizing them in a greenhouse environment with a shading rate of 85% and a humidity of 100%;

[0018] (2) Soak the tissue culture seedlings in nutrient solution A during transplanting, and immediately irrigate the roots with nutrient solution A after acclimatization and transplanting. The root irrigation amount per mu is 100-200 kg;

[0019] (3) 7 to 10 days after the tissue culture seedlings are acclimated and transplanted, use nutrient solution B for root irrigation, with an irrigation amount of 200 to 300 kg per mu;

[0020] (4) 20 to 25 days after the tissue culture seedlings are acclimated and transplanted, use nutrient solution C for root irrigation at a rate of 200 to 300 kg per mu. After root irrigation, rinse the leaves with clean water until no obvious nutrient solution C remains.

[0021] (5) After implementing step (4), irrigate the roots with nutrient solution C every 10 to 15 days, with an irrigation rate of 200 to 300 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious residue of nutrient solution C.

[0022] Further, the steps include:

[0023] (1) Transplanting the tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation into a mixed substrate and acclimatizing them in a greenhouse environment with a shading rate of 85% and a humidity of 100%;

[0024] (2) Use nutrient solution A to soak tissue culture seedlings during transplanting, and immediately irrigate roots with nutrient solution A after acclimatization and transplanting, with a root irrigation rate of 150 kg per mu;

[0025] (3) Eight days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution B, with an irrigation amount of 250 kg per mu;

[0026] (4) 22 days after the acclimatization and transplanting of the tissue culture seedlings, irrigate the roots with nutrient solution C at a rate of 250 kg per mu. After irrigating the roots, rinse the leaves with clean water until no obvious nutrient solution C remains.

[0027] (5) After implementing step (4), irrigate the roots with nutrient solution C every 14 days, with an irrigation rate of 250 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious residue of nutrient solution C.

[0028] Furthermore, the mixed matrix formula is: humus, coconut bran and sheep manure, and the volume ratio thereof is 5:2:0.1-0.2.

[0029] The beneficial technical effect of the present invention is that no matter whether it is seedling growth or pseudobulb expansion, there is a synergistic effect between the mushroom and the bacteria. The mushroom is obtained through tissue culture, and no matter it is the stems, leaves or roots, it almost does not carry microorganisms. When the tissue culture seedlings are transplanted into the soil, they cannot absorb the symbiotic bacteria well and quickly, so that the mushroom has a low survival rate, extremely slow root growth and delayed pseudobulb expansion during domestication and hardening. According to the investigation of the members of the present invention on the ecological environment of the wild growth of the mushroom, it was found that the mushroom under the pine forest with a deep humus layer and the broad-leaved forest under the mushroom The mushrooms are distributed in large numbers, grow well and are relatively large in size, especially near the root layer of trees. At the beginning of the present invention, the humus soil under the forest where the hairy mushroom grows was tried as the domestication matrix or added to the domestication matrix. It has the effect of improving the domestication survival rate of the hairy mushroom tissue culture seedlings and promoting growth, but it is not particularly obvious. At the same time, there are a large number of pathogenic microorganisms carried in the humus soil, which can easily cause the occurrence of stem base rot and leaf spot during the domestication of the hairy mushroom tissue culture seedlings. In addition, due to the extremely limited distribution of hairy mushrooms, the humus soil resources in its habitat are also extremely limited. The present invention creatively adopts a suspension prepared by pine tree roots, and adds a certain concentration of NAA and IBA, soaks at the beginning of cultivation, and regularly irrigates after cultivation, which can quickly promote the growth of the root system of Radix Sagittiflorae, is beneficial to the absorption of water by Radix Sagittiflorae, and can greatly improve the survival rate of Radix Sagittiflorae domestication. At the same time, the growth of the root system is beneficial to the intake of nutrients, and in the middle and late stages, a nutrient solution composed of sheep manure fermentation liquid, humic acid, water-soluble compound fertilizer and potassium dihydrogen phosphate is used to promote the pseudobulb of Radix Sagittiflorae to expand rapidly and grow into commercial seedlings in a short period of time. Its mechanism is that the suspension prepared by the pine tree root system has symbiotic bacteria for the growth of Radix Sagittiflorae, and is matched with a certain concentration of NAA and IBA, which can effectively promote the elongation of the original root system of Radix Sagittiflorae and the occurrence of new root systems. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0031] Figure 1 It is a view of the survival rate after acclimation for 3 months of the present invention.

[0032] Figure 2 It is a view of the pseudobulb of the Mao Sagittaria tissue culture seedlings of the present invention after acclimatization for 3 months. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Example 1

[0035] A nutrient solution for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia, comprising: a nutrient solution A for soaking tissue culture seedlings or irrigating roots during tissue culture seedling acclimation and transplantation, a nutrient solution B for irrigating roots 7 days after tissue culture seedling acclimation and transplantation, and a nutrient solution C for irrigating roots 20 days after tissue culture seedling acclimation and transplantation;

[0036] The formula of nutrient solution A is: pine root suspension 4.0ml / L + NAA 5.0mg / L + IBA 1.0mg / L;

[0037] The formula of nutrient solution B is: pine root suspension 10.0ml / L + NAA 30.0mg / L + IBA 10.0mg / L;

[0038] The formula of nutrient solution C is: sheep manure fermentation liquid 10.0ml / L + humic acid 0.6g / L + water-soluble compound fertilizer 1.0g / L + potassium dihydrogen phosphate 0.5g / L.

[0039] Among them, the mass ratio of nitrogen, phosphorus and potassium in water-soluble compound fertilizer is 20:20:20.

[0040] The preparation method of the pine root suspension is as follows: take 2 g of pine root, add 1 L of water, and use a soymilk machine to squeeze it at room temperature for 5 minutes.

[0041] The preparation method of the sheep manure fermentation liquid is as follows: 100 g of the fermented sheep manure is taken, 1 L of water is added, the mixture is sealed and stored for 1 month, and then the mixture is heated to boiling and then cooled to room temperature.

[0042] Example 2

[0043] A nutrient solution for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia, comprising: a nutrient solution A for soaking tissue culture seedlings or irrigating roots during tissue culture seedling acclimation and transplantation, a nutrient solution B for irrigating roots 10 days after tissue culture seedling acclimation and transplantation, and a nutrient solution C for irrigating roots 25 days after tissue culture seedling acclimation and transplantation;

[0044] The formula of nutrient solution A is: pine root suspension 5.0ml / L + NAA 6.0mg / L + IBA2.0mg / L;

[0045] The formula of nutrient solution B is: pine root suspension 12.0ml / L+NAA 35.0mg / L+IBA 15.0mg / L;

[0046] The formula of nutrient solution C is: sheep manure fermentation liquid 20.0ml / L + humic acid 0.8g / L + water-soluble compound fertilizer 1.2g / L + potassium dihydrogen phosphate 0.8g / L.

[0047] Among them, the mass ratio of nitrogen, phosphorus and potassium in water-soluble compound fertilizer is 20:20:20.

[0048] The preparation method of the pine root suspension is as follows: take 4 g of pine root, add 1 L of water, and squeeze it with a soymilk machine at room temperature for 10 minutes.

[0049] The preparation method of the sheep manure fermentation liquid is as follows: 200 g of the fermented sheep manure is taken, 1 L of water is added, and the mixture is sealed and stored for 1 month, and then heated to boiling and cooled to room temperature.

[0050] Example 3

[0051] A nutrient solution for promoting rapid growth of tissue culture seedlings of Agaricus quinquefolius, comprising: a nutrient solution A for soaking tissue culture seedlings or irrigating roots during tissue culture seedling acclimation and transplantation, a nutrient solution B for irrigating roots 8 days after tissue culture seedling acclimation and transplantation, and a nutrient solution C for irrigating roots 23 days after tissue culture seedling acclimation and transplantation;

[0052] The formula of nutrient solution A is: pine root suspension 4.5ml / L + NAA 5.5mg / L + IBA1.1mg / L;

[0053] The formula of nutrient solution B is: pine root suspension 11.0ml / L + NAA32.0mg / L + IBA 13.0mg / L;

[0054] The formula of nutrient solution C is: sheep manure fermentation liquid 11.0ml / L + humic acid 0.7g / L + water-soluble compound fertilizer 1.1g / L + potassium dihydrogen phosphate 0.6g / L;

[0055] Among them, the mass ratio of nitrogen, phosphorus and potassium in water-soluble compound fertilizer is 20:20:20.

[0056] The preparation method of the pine root suspension is as follows: take 3 g of pine root, add 1 L of water, and use a soymilk machine to squeeze it at room temperature for 7 minutes.

[0057] The preparation method of the sheep manure fermentation liquid is as follows: 150 g of the fermented sheep manure is taken, 1 L of water is added, and the mixture is sealed and stored for 1 month, and then heated to boiling and cooled to room temperature.

[0058] Example 4

[0059] A method for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia, wherein the nutrient solution adopts the formula of Example 1, and the steps include:

[0060] (1) The tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation were transplanted into a mixed matrix and domesticated in a greenhouse environment with a shading rate of 85% and a humidity of 100%; the formula of the mixed matrix was humus soil, coconut bran and sheep manure, with a volume ratio of 5:2:0.2.

[0061] (2) When transplanting, soak the tissue culture seedlings in nutrient solution A, and immediately irrigate the roots with nutrient solution A after acclimatization and transplanting. The root irrigation amount per mu is 100 kg.

[0062] (3) Seven days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution B at a rate of 200 kg per mu.

[0063] (4) 20 days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution C at a rate of 200 kg per mu. After irrigating the roots, the leaves were rinsed with clean water until no obvious nutrient solution C remained.

[0064] (5) After implementing step (4), irrigate the roots with nutrient solution C every 10 days, with an irrigation amount of 200 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious nutrient solution C residue.

[0065] After 3 months of domestication, the survival rate was 99.5%, and the average diameter of the pseudobulbs was 0.74 cm, with the largest being 1.21 cm and the smallest being 0.61 cm.

[0066] Example 5

[0067] A method for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia using a nutrient solution, wherein the nutrient solution adopts the formula of Example 2, and the steps include:

[0068] (1) The tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation were transplanted into a mixed matrix and domesticated in a greenhouse environment with a shading rate of 85% and a humidity of 100%; the formula of the mixed matrix was: humus, coconut bran and sheep manure, with a volume ratio of 5:2:0.1.

[0069] (2) When transplanting, soak the tissue culture seedlings in nutrient solution A, and immediately irrigate the roots with nutrient solution A after acclimatization and transplanting. The root irrigation amount per mu is 200 kg.

[0070] (3) Ten days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution B, with an irrigation amount of 300 kg per mu.

[0071] (4) 25 days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution C at a rate of 300 kg per mu. After irrigating the roots, the leaves were rinsed with clean water until no obvious nutrient solution C remained.

[0072] (5) After implementing step (4), irrigate the roots with nutrient solution C every 15 days, with an irrigation rate of 200 to 300 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious residue of nutrient solution C.

[0073] After 3 months of domestication, the survival rate was 99.8%, and the average diameter of the pseudobulbs was 0.71 cm, with the largest being 1.31 cm and the smallest being 0.59 cm.

[0074] Example 6

[0075] A method for promoting rapid growth of tissue culture seedlings of Radix Sagittifolia, wherein the nutrient solution adopts the formula of Example 3, and the steps include:

[0076] (1) The tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation were transplanted into a mixed matrix and domesticated in a greenhouse environment with a shading rate of 85% and a humidity of 100%; the formula of the mixed matrix was: humus, coconut bran and sheep manure, with a volume ratio of 5:2:0.1.

[0077] (2) When transplanting, use nutrient solution A to soak the tissue culture seedlings, and immediately irrigate the roots with nutrient solution A after acclimatization and transplanting. The root irrigation amount per mu is 150 kg.

[0078] (3) Eight days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution B at a rate of 250 kg per mu.

[0079] (4) 22 days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution C at a rate of 250 kg per mu. After irrigating the roots, the leaves were rinsed with clean water until no obvious nutrient solution C remained.

[0080] (5) After implementing step (4), irrigate the roots with nutrient solution C every 14 days, with an irrigation rate of 250 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious residue of nutrient solution C.

[0081] After 3 months of domestication, the survival rate was 99.2%, and the average diameter of the pseudobulbs was 0.81 cm, with the largest being 1.52 cm and the smallest being 0.72 cm.

[0082] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A nutrient solution for promoting the rapid growth of tissue culture seedlings of Radix Sagittifolia, characterized in that: include: Nutrient solution A for soaking tissue culture seedlings or irrigating roots during acclimation and transplantation of tissue culture seedlings, nutrient solution B for irrigating roots 7 to 10 days after acclimation and transplantation of tissue culture seedlings, and nutrient solution C for irrigating roots 20 to 25 days after acclimation and transplantation of tissue culture seedlings; The formula of the nutrient solution A is: pine root suspension 4.0-5.0 ml / L + NAA 5.0-6.0 mg / L + IBA 1.0-2.0 mg / L; The formula of the nutrient solution B is: pine root suspension 10.0-12.0 ml / L + NAA 30.0-35.0 mg / L + IBA 10.0-15.0 mg / L; The formula of the nutrient solution C is: 10.0-20.0 ml / L of sheep manure fermentation liquid + 0.6-0.8 g / L of humic acid + 1.0-1.2 g / L of water-soluble compound fertilizer + 0.5-0.8 g / L of potassium dihydrogen phosphate; The mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20:20:20; The preparation method of the sheep manure fermentation liquid is as follows: 100-200 g of the fermented sheep manure is taken, 1 L of water is added, the mixture is sealed and stored for 1 month, and then the mixture is heated to boiling and then cooled to room temperature to obtain the fermented sheep manure.

2. The nutrient solution according to claim 1, characterized in that: The formula of the nutrient solution A is: pine root suspension 4.5 ml / L + NAA 5.5 mg / L + IBA 1.1 mg / L; The formula of the nutrient solution B is: pine root suspension 11.0 ml / L + NAA 32.0 mg / L + IBA 13.0 mg / L; The formula of the nutrient solution C is: 11.0 ml / L of sheep manure fermentation liquid + 0.7 g / L of humic acid + 1.1 g / L of water-soluble compound fertilizer + 0.6 g / L of potassium dihydrogen phosphate.

3. The nutrient solution according to claim 2, characterized in that: The preparation method of the pine root suspension is as follows: 2 to 4 g of pine root is added with 1 L of water, and the suspension is squeezed at room temperature for 5 to 10 minutes using a soybean milk machine.

4. A method for promoting the rapid growth of Pleurotus eryngii tissue culture seedlings using the nutrient solution of claim 1 or 2, characterized in that the steps include: (1) Transplanting the tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation into a mixed substrate and acclimatizing them in a greenhouse environment with a shading rate of 85% and a humidity of 100%; (2) Soak the tissue culture seedlings in nutrient solution A during transplanting, and immediately irrigate the roots with nutrient solution A after acclimatization and transplanting. The root irrigation amount per mu is 100-200 kg; (3) 7 to 10 days after the tissue culture seedlings are acclimated and transplanted, use nutrient solution B to irrigate the roots, with an irrigation amount of 200 to 300 kg per mu; (4) 20 to 25 days after the tissue culture seedlings are acclimated and transplanted, use nutrient solution C for root irrigation at a rate of 200 to 300 kg per mu. After root irrigation, rinse the leaves with clean water until no obvious nutrient solution C remains. (5) After implementing step (4), irrigate the roots with nutrient solution C every 10 to 15 days, with an irrigation rate of 200 to 300 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious residue of nutrient solution C.

5. The method according to claim 4, characterized in that the steps include: (1) Transplanting the tissue culture seedlings of Radix Sagittifolia obtained by one-step seedling formation into a mixed substrate and acclimatizing them in a greenhouse environment with a shading rate of 85% and a humidity of 100%; (2) Use nutrient solution A to soak tissue culture seedlings during transplanting, and immediately irrigate roots with nutrient solution A after acclimatization and transplanting, with a root irrigation rate of 150 kg per mu; (3) Eight days after the tissue culture seedlings were acclimated and transplanted, the roots were irrigated with nutrient solution B, with an irrigation amount of 250 kg per mu; (4) 22 days after the acclimatization and transplanting of the tissue culture seedlings, irrigate the roots with nutrient solution C at a rate of 250 kg per mu. After irrigating the roots, rinse the leaves with clean water until no obvious nutrient solution C remains. (5) After implementing step (4), irrigate the roots with nutrient solution C every 14 days, with an irrigation rate of 250 kg per mu. After irrigating the roots, rinse the leaves with clean water until there is no obvious residue of nutrient solution C.

6. The method according to claim 5, characterized in that: The mixed matrix formula is: humus soil, coconut bran and sheep manure, and the volume ratio thereof is 5:2:0.1-0.2.

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