Side wall adsorption type magnetic separation chemiluminescence characteristic detection device

Abstract

The invention discloses a side wall adsorption type magnetic separation chemiluminescence characteristic detection device. The device comprises a base, and an incubation module, a pipetting module, a magnetic suction module and a detection module which are mounted on the base. The magnetic attraction module comprises a guide support, a magnetic attraction base plate, a magnetic attraction displacement driving assembly and a magnetic structure. The magnetic attraction base plate is connected to the guide support and is driven by the magnetic attraction displacement driving assembly to move. The magnetic structure is mounted on the magnetic substrate. The magnetic attraction base plate is provided with a magnetic attraction position and an avoiding position. In a state that the pipetting module corresponds to the position of the light-emitting detection hole of the reagent strip, the magnetic structure on the magnetic attraction substrate at the magnetic attraction position is close to the outer side wall of the light-emitting detection hole of the reagent strip, and a magnetic attraction force enabling the magnetic bead to be attached to the wall is generated on the magnetic bead. The light-emitting detection holes are formed in the end parts of the reagent holes, so that the outer side walls of the light-emitting detection holes in a plurality of reagent strips which are arranged side by side are exposed, the magnetic beads of the plurality of reagent strips are magnetically attracted and fixed at the same time, and multi-channel detection is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of sample detection, and in particular relates to a sidewall adsorption type magnetic separation chemiluminescence characteristic detection device. Background Art

[0002] The single-dose chemiluminescence feature is that it tests one item per person, which is different from the traditional tube chemiluminescence (the reagents are large-sized bottled reagents). The single-dose chemiluminescence reagents are independently packaged, which integrates the reagents and consumables required for the test on a single-dose reagent card. The single-dose chemiluminescence equipment has no liquid path system and is maintenance-free. While being applied to traditional POCT (point-of-care testing, instant testing) scenarios, it also expands the scope of detectable items.

[0003] The commonly used magnetic suction method for single-portion chemiluminescence is to suck the magnetic beads into the TIP tip, and then use a permanent magnet to cling to the outer wall of the TIP tip to adsorb the magnetic beads. After the adsorption is completed, the waste liquid is spit out of the TIP tip, thereby achieving the purpose of continuously cleaning the magnetic beads and their adsorbents and improving the performance of the instrument. However, the conventional magnetic suction method requires the magnetic beads to be repeatedly sucked into the TIP tip, which increases the risk of magnetic bead loss. The traditional chemiluminescence machine has a relatively complex structure, a relatively large size, relatively more movements between the various components, and a complex process.

[0004] The common magnetic absorption method for conventional single-portion chemiluminescence is to absorb the magnetic beads into the TIP tip, and then use a permanent magnet to cling to the outer wall of the TIP tip to absorb the magnetic beads. This solution has a relatively complex structure and design, and occupies a large space. In addition, when the TIP tip absorbs the magnetic bead liquid, there is a residual risk, which increases the possibility of magnetic bead loss. And from the perspective of the entire adsorption process, the process action is also relatively complicated. During the repeated cleaning process, the magnetic beads need to be continuously sucked in and spit out of the TIP tip. As the number of times increases, the magnetic bead loss further increases. Summary of the invention

[0005] The purpose of the present invention is to provide a side wall adsorption type magnetic separation chemiluminescence characteristic detection device.

[0006] A side wall adsorption type magnetic separation chemiluminescence characteristic detection device comprises a base, and an incubation module, a pipetting module, a magnetic suction module and a detection module installed on the base. The incubation module is used to store and move a reagent strip. The pipetting module is used to transfer reagents in different wells of the reagent strip. The detection module is used to perform luminescence characteristic detection on the luminescence detection well of the reagent strip.

[0007] The magnetic module comprises a guide bracket, a magnetic substrate, a magnetic displacement driving component and a magnetic structure. The magnetic substrate is connected to the guide bracket and moves under the drive of the magnetic displacement driving component. The magnetic structure is installed on the magnetic substrate.

[0008] The magnetic attraction substrate has two working positions, namely the magnetic attraction position and the avoidance position. When the position of the pipetting module corresponds to the position of the luminescent detection hole of the reagent strip, the magnetic structure on the magnetic attraction substrate in the magnetic attraction position is close to the outer wall of the luminescent detection hole of the reagent strip, and generates a magnetic attraction force on the magnetic beads in the luminescent detection hole to make the magnetic beads adhere to the wall. The magnetic structure on the magnetic attraction substrate in the avoidance position is away from the outer wall of the luminescent detection hole of the reagent strip, does not affect the pipetting module to absorb the magnetic beads in the luminescent detection hole, and does not hinder the relative movement of the incubation module and the pipetting module.

[0009] Preferably, during the working process, the separation of magnetic beads from the sample liquid and the cleaning of the magnetic beads are carried out in the luminescence detection hole; the process of separating the magnetic beads from the sample liquid and the cleaning liquid is: first move the magnetic attraction substrate to the magnetic attraction position, so that the magnetic beads in the luminescence detection hole are adsorbed on the inner wall of the luminescence detection hole by the permanent magnet; then use the pipetting module to suck out the sample liquid or cleaning liquid from the luminescence detection hole.

[0010] Preferably, when the magnetic substrate is in the avoidance position, the bottom surface of the magnetic substrate is higher than the top of the incubation module.

[0011] Preferably, the incubation module comprises a slide, a reagent carrying box, a tip box and a reagent lateral movement drive assembly. The slide is slidably connected to the base and driven by the reagent lateral movement drive assembly. The reagent carrying box and the tip box are both mounted on the slide. One or more reagent strip mounting positions are provided on the reagent carrying box. Each reagent strip mounting position corresponds to one or more tip storage holes on the tip box.

[0012] Preferably, the reagent carrying box is provided with a plurality of reagent strip installation positions arranged side by side; in the reagent strip installed in the reagent strip installation position, the luminescent detection hole is located at the end of the reagent strip farthest from the tip box. The magnetic structure on the magnetic attraction substrate in the magnetic attraction position is aligned with each reagent strip along the length direction of the reagent strip.

[0013] Preferably, the magnetic structures on the magnetic substrate are equal in number to the reagent strip installation positions on the reagent carrying box and correspond one to one. A groove structure matching the shape of the outer wall of the luminescent detection hole is provided on the outer surface of the magnetic structure.

[0014] Preferably, the incubation module further comprises an incubation rail and a guide rod. The incubation rail and the guide rod are parallel to each other and are fixed on the base. Both sides of the bottom of the slide are slidably connected to the incubation rail and the guide rod respectively.

[0015] Preferably, a heating component is installed inside the reagent carrying box or on the slide.

[0016] Preferably, the magnetic structure adopts a permanent magnet or an electromagnet.

[0017] Preferably, the pipetting module comprises a pipetting support, a lifting slide, a pipetting gun, a membrane breaking head and a pipetting lifting drive assembly. The pipetting support is fixed on the support frame. The lifting slide is slidably connected to the pipetting support and driven by the pipetting lifting drive assembly. The lifting slide is equipped with a pipetting gun and a membrane breaking head corresponding to the reagent strip installation position.

[0018] Preferably, it also includes a printer, a USB interface and a network interface.

[0019] Preferably, the sliding direction of the magnetic substrate is an inclined direction gradually approaching the reagent carrying box from top to bottom.

[0020] Preferably, the reagent transverse drive assembly comprises a roller, a transverse drive motor and a track plate. The track plate is fixed on the base; the roller is rotatably connected to the slide, and the outer circumference is rollingly connected to the track plate; the roller is driven by the transverse drive motor.

[0021] The present invention has the following beneficial effects: 1. The present invention adsorbs magnetic beads on the inner wall of the luminescent detection hole by setting a magnetic structure corresponding to the position of the luminescent detection hole of the reagent strip, so that the pipetting module can absorb the sample liquid or cleaning liquid in the luminescent detection hole without affecting the magnetic beads, thereby avoiding the loss of magnetic beads caused by multiple transfers of magnetic beads to different hole positions in the existing detection device, thereby effectively improving the accuracy of chemiluminescent characteristic detection.

[0022] 2. The present invention arranges the luminescence detection hole at the end of the reagent hole, so that the outer side walls of the luminescence detection holes on multiple reagent strips placed side by side are exposed, so as to facilitate the magnetic fixation of magnetic beads in multiple reagent strips at the same time, thereby realizing multi-channel chemiluminescence characteristic detection.

[0023] 3. The magnetic bead separation process of the present invention only requires two steps: magnetic suction and sample liquid aspiration. Compared with the prior art, which involves aspirating the magnetic bead mixture, magnetically aspirating the tip, releasing the liquid from the tip, and transferring the magnetic beads in the tip to the next well, the operation process is simplified. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is the first overall structural schematic diagram of the present invention.

[0025] Figure 2 It is a second overall structural schematic diagram of the present invention.

[0026] Figure 3This is the first structural schematic diagram of the pipetting module in the present invention.

[0027] Figure 4 This is a second structural schematic diagram of the pipetting module in the present invention.

[0028] Figure 5 It is a schematic diagram of the working state of the magnetic attraction module in the present invention.

[0029] Figure 6 It is a schematic diagram of the luminescence detection hole of the photomultiplier tube in the present invention for detection.

[0030] Figure numerals: 1, base; 1-1, support foot; 2, support frame; 3, incubation module; 3-1, incubation guide rail; 3-2, guide rod; 3-3, slide; 3-4, reagent carrying box; 3-5, tip box; 3-6, reagent lateral movement drive assembly; 3-6-1, roller; 3-6-2, rail plate; 4, pipetting module; 4-1, pipetting bracket; 4-2, lifting slide; 4-3, pipetting gun; 4-4, pipetting lifting drive assembly; 5, magnetic suction module; 5-1, guide bracket; 5-2, magnetic substrate; 5-3, magnetic displacement drive component; 5-4, magnetic structure; 6, detection module; 6-1, two-axis drive component; 6-2, detection substrate; 6-3, photomultiplier tube; 7, reagent strip; 7-1, sample hole; 7-2, luminescent detection hole; 8, suction head; 9, printer; 10, filter; 11, power supply component; 12, USB interface; 13, network interface; 14, control board. DETAILED DESCRIPTION

[0031] The present invention will be further described below in conjunction with the accompanying drawings.

[0032] like Figure 1 and Figure 2 As shown, a side wall adsorption type magnetic separation chemiluminescence characteristic detection device includes a base 1, a support frame 2, an incubation module 3, a pipetting module 4, a magnetic suction module 5, a detection module 6, a printer 9, a filter 10 and a control module. The support frame 2 is fixed on the base 1. A support foot 1-1 is installed at the bottom of the base 1.

[0033] like Figure 3 and Figure 4 As shown, the incubation module 3 includes an incubation rail 3-1, a guide rod 3-2, a slide 3-3, a reagent carrying box 3-4, a tip box 3-5, a heating component and a reagent lateral drive component 3-6. The incubation rail 3-1 and the guide rod 3-2, which are parallel to each other, are fixed on the base 1, and are used to provide guidance and load-bearing for the slide 3-3 and the reagent carrying box 3-4. The two sides of the bottom of the slide 3-3 are slidably connected to the incubation rail 3-1 and the guide rod 3-2 respectively. The slide 3-3 slides in a linear reciprocating motion under the drive of the reagent lateral drive component 3-6.

[0034] In some embodiments, the reagent transverse drive assembly 3-6 includes a roller 3-6-1, a transverse drive motor and a track plate 3-6-2. The track plate 3-6-2 is fixed on the base 1; the roller 3-6-1 is rotatably connected to the slide 3-3, and the outer circumference is rollingly connected to the track plate 3-6-2; the transverse drive motor is fixed on the slide 3-3, and the output shaft is coaxially fixed with the roller 3-6-1.

[0035] The tip box 3-5 and the reagent carrying box 3-4 are installed on the top of the slide 3-3. The tip box 3-5 is placed in the groove structure on the slide 3-3 and can be removed. The tip box 3-5 is located on the side of the reagent carrying box 3-4 away from the magnetic suction module 5. The reagent carrying box 3-4 is provided with a plurality of reagent strip mounting positions arranged in sequence along the direction of the axis of the vertical guide rod 3-2. One or more rows of tip storage holes are provided on the tip box 3-5. Each row of tip storage holes includes a plurality of tip storage holes corresponding to each reagent strip mounting position one by one. During the movement, the slide 3-3 can stay at any position on the straight line, which is convenient for completing the detection action in the single-person chemiluminescence instrument and improving the efficiency of the instrument. The side-by-side multi-channel structure design of the reagent carrying box 3-4 can test multiple reagent strips 7 at the same time, improve the test efficiency, and reduce the size of the whole machine structure.

[0036] The reagent strip installation position adopts a slot structure; the reagent strip 7 is inserted from the end of the reagent strip installation position close to the tip box 3-5. When the reagent strip 7 is inserted, the tip box 3-5 is taken out to prevent the tip box 3-5 from blocking the insertion of the reagent strip 7. The edges of the top surface of the reagent strip 7 inserted into the reagent strip installation position are constrained by the flange structure at the top of the reagent strip installation position; the end of each reagent strip installation position away from the tip box 3-5 is provided with a reagent strip limiting structure for accurate positioning of the reagent strip 7.

[0037] The reagent strip 7 used in this embodiment has a total of ten wells, which are divided into a sample well 7-1, eight reagent wells and a luminescent detection well 7-2 arranged in sequence. The eight reagent wells include two lysis wells, a substrate well, three wash wells, an enzyme label well and a magnetic bead well. During the detection process, the luminescent detection well 7-2 is located at the end of the reagent strip 7 farthest from the tip box 3-5. The top openings of all wells on the reagent strip are provided with sealing films. The sealing film is preferably an aluminum film.

[0038] The heating component is installed inside the reagent carrying box 3-4 or on the top of the slide 3-3, and is used to heat the reagent strip 7. In this embodiment, the heating component is a heating sheet.

[0039] like Figure 2As shown, the pipetting module 4 includes a pipetting support 4-1, a lifting slide 4-2, a pipetting gun 4-3, a membrane breaking head and a pipetting lifting drive assembly 4-4. The pipetting support 4-1 is fixed on the support frame 2. The lifting slide 4-2 is slidably connected to the pipetting support 4-1 through a pipetting lifting guide rail. The lifting slide 4-2 is driven by the pipetting lifting drive assembly 4-4 to perform lifting and lowering movements. A plurality of pipetting guns 4-3, which are equal to and correspond to the number of reagent strip installation positions, and a plurality of membrane breaking heads are installed on the lifting slide 4-2.

[0040] In this embodiment, the liquid transfer lifting drive assembly 4-4 drives the lifting slide 4-2 to move up and down through a motor. The specific driving structure belongs to the prior art and can adopt a screw nut structure, a synchronous belt structure or other existing transmission structures, which will not be described in detail here. The reagent lateral movement drive assembly 3-6 and the liquid transfer lifting drive assembly 4-4 cooperate with each other so that the membrane breaking head and the liquid transfer gun 4-3 can move to any luminescent detection hole 7-2 and reagent hole of the corresponding reagent strip 7. The membrane breaking head is used to puncture the aluminum film on each hole of the reagent strip 7. The liquid transfer gun 4-3 is used for the transfer, quantification and reaction of reagents.

[0041] like Figure 5 As shown, the magnetic module 5 is installed on the support frame 2, including a guide bracket 5-1, a magnetic substrate 5-2, a magnetic displacement drive component 5-3, and one or more magnetic structures 5-4. The inclined guide bracket 5-1 is fixed on the support frame 2. The magnetic substrate 5-2 is slidably connected to the guide bracket 5-1 through a slider and a magnetic guide rail, and is driven by the magnetic displacement drive component 5-3 to slide back and forth. The sliding direction of the magnetic substrate 5-2 is from top to bottom and gradually approaches the inclined direction of the reagent carrying box 3-4.

[0042] In some embodiments, the magnetic displacement drive assembly 5-3 includes a magnetic displacement motor and a first screw. The magnetic displacement motor is fixed on the guide bracket 5-1; the first screw is rotatably connected to the guide bracket 5-1. The output shaft of the magnetic displacement motor is fixed to the end of the first screw; the first screw and the nut fixed on the magnetic substrate 5-2 form a screw pair.

[0043] One or more magnetic structures 5-4 are fixed to the side of the magnetic substrate 5-2 close to the carrier box. In some embodiments, the number of magnetic structures 5-4 and the reagent strip mounting positions on the reagent carrier box 3-4 are equal and correspond one to one. A groove structure matching the shape of the outer wall of the luminescence detection hole 7-2 is provided on the outer surface of the magnetic structure 5-4, so that the magnetic structure 5-4 can fit tightly with the outer wall of the luminescence detection hole 7-2, thereby increasing the suction force on the magnetic beads in the luminescence detection hole 7-2. Furthermore, the outer wall of the luminescence detection hole 7-2 is a cylindrical surface, and the groove structure on the magnetic structure 5-4 is arc-shaped.

[0044] The magnetic substrate 5-2 has two working positions, namely the magnetic position and the avoidance position. When the magnetic substrate 5-2 is in the magnetic position and the luminescent detection hole 7-2 at the end of the reagent strip 7 is directly below the pipette gun 4-3, the magnetic structure 5-4 on the magnetic substrate 5-2 presses against the outer wall of the luminescent detection hole 7-2 on the reagent strip 7. At this time, the magnetic structure 5-4 can generate suction to the magnetic beads in the luminescent detection holes 7-2 at the ends of all reagent strips 7 on the reagent carrier box 3-4; so that the magnetic beads are tightly attached to the side walls of the luminescent detection holes 7-2 and will not be sucked away by the pipette gun 4-3; thus, different reagents can be injected and sucked out of the luminescent detection holes 7-2 without affecting the magnetic beads, thereby completing the entire chemiluminescent characteristic test task in one hole, avoiding the problem of magnetic bead loss caused by multiple transfers of magnetic beads in the prior art.

[0045] When the magnetic substrate 5 - 2 is in the avoidance position, the bottom surface of the magnetic substrate 5 - 2 is higher than each reagent strip 7 and will not block the reciprocating movement of the reagent strip 7 .

[0046] During operation, when the incubation module 3 moves to the designated position, the magnetic suction module 5 drives the magnetic structure to move downward obliquely. When the magnetic structure moves to the vicinity of the luminescent detection hole 7-2, it stops moving. After a period of time, the magnetic beads in the luminescent detection hole are tightly adsorbed on the side wall of the luminescent detection hole 7-2, and then enter the luminescent detection hole 7-2 through the pipetting module 4 to absorb the waste liquid. After completion, the waste liquid is spit to the designated position, and then new cleaning liquid is sucked and spit into the luminescent detection hole 7-2 for cleaning. During the cleaning process, the magnetic structure is controlled by the motor to move upward to the initial position to release the magnetic beads. This reciprocating operation is performed multiple times to achieve the purpose of cleaning the magnetic beads. During the whole process, the magnetic beads are always in the luminescent detection hole 7-2, and there is no need to suck the TIP suction head 8, which improves the performance of the instrument.

[0047] In this embodiment, the magnetic structure 5-4 is a magnetic structure; in some other embodiments, the magnetic structure can be replaced by an electromagnet. The magnetic attraction of the magnetic beads in the luminescent detection hole 7-2 is controlled by turning the electromagnet on and off.

[0048] The detection module 6 includes a two-axis driving assembly 6-1, a detection substrate 6-2, a barcode scanner and photomultiplier tubes 6-3 (PMT). The photomultiplier tubes 6-3 are all installed on the detection substrate 6-2. The two-axis driving assembly 6-1 is used to drive the photomultiplier tubes 6-3 to move horizontally and vertically, so that the photomultiplier tubes 6-3 can reach the top of the luminescent detection holes 7-2 of all reagent strips 7 for detection. A light-shielding cover is provided at the detection port of the photomultiplier tube 6-3. The light-shielding cover is cylindrical; and the bottom opening of the light-shielding cover can be placed on the outside of the top opening of the luminescent detection hole 7-2 of the reagent strip by moving up and down, so as to block the influence of external ambient light on the detection results.

[0049] In this embodiment, the two-axis drive assembly 6-1 includes a detection transverse drive assembly and a detection lifting drive assembly. The detection transverse drive assembly adopts a synchronous belt transmission structure driven by a motor to drive a transverse plate to move horizontally. The detection lifting drive assembly is installed on the transverse plate, and adopts a screw transmission structure driven by a motor to drive a lifting plate to move vertically. The detection substrate 6-2 is installed on the lifting plate. The barcode scanner is fixed on the detection substrate 6-2, or fixed on the transverse plate driven by the detection transverse drive assembly, so that the barcode scanner can move horizontally for different barcodes or QR codes on different reagent strips.

[0050] like Figure 1 and Figure 6 As shown, the detection module 6 is used to detect the processed sample in the luminescent detection hole 7-2. When the reagent lateral drive component 3-6 drives the reagent carrying box 3-4 to move, so that the reagent strip 7 reaches the detection position; the detection module 6 moves horizontally under the drive of the two-axis drive component 6-1, and scans the entire row of reagent strips 7 in sequence through the barcode scanner, so as to identify each reagent strip 7; then the detection module 6 drives the photomultiplier tube 6-3 to move horizontally and vertically to the specified position to detect the luminescent detection hole 7-2 of each reagent strip 7.

[0051] The control module includes a power supply component 11, a USB interface 12, a network interface 13 and a control board 14. The power supply component 11 and the control board 14 are installed on the top of the support frame 2. The power supply component 11 includes a DC power supply. The filter 10, the printer 9, the USB interface 12, and the network interface 13 are installed on the side of the support frame 2. The filter 10 is used to connect the external power line and filter the external input voltage to power the whole machine. The printer 9 is used to print the test results.

[0052] The detection method of the sidewall adsorption type magnetic separation chemiluminescence characteristic detection device comprises the following steps: Step 1: When the staff needs to test, the detection system is started, and the reagent lateral drive assembly 3-6 in the incubation module 3 drives the tip box 3-5 to move to the exit position of the whole machine; the staff takes out the tip box 3-5 and installs the TIP tip 8 on the tip box 3-5; the test reagent strip 7 with the sample liquid added to the sample hole 7-1 is pushed into each reagent strip installation position in the reagent carrying box 3-4. The reagent strip limiting structure on the reagent strip installation position provides a limit for the test reagent strip 7, so as to confirm the push-in status of the reagent strip 7 and solve the risk caused by the reagent strip 7 not being pushed into the specified position. The tip box 3-5 containing the new TIP tip 8 is placed on the slide 3-3.

[0053] Step 2: The heating component in the incubation module 3 heats each reagent strip 7 to a specified temperature to improve the detection reaction efficiency; the temperature of the reagent strip 7 is detected and controlled in real time by the temperature control switch. The reagent lateral drive component 3-6 drives the reagent carrying box 3-4 to move to the bottom of the detection module 6; the two-axis drive component 6-1 in the detection module 6 drives the barcode scanner to move to the top of the QR code or barcode of each reagent strip 7 in turn and scans the code one by one to collect the information of each reagent strip 7.

[0054] Step three, the reagent transverse movement drive component 3-6 drives the reagent carrying box 3-4 to move to the bottom of the pipetting module 4; through the cooperation of the reagent transverse movement drive component 3-6 and the pipetting lifting drive component 4-4, the membrane piercing head pierces all the luminescent detection holes 7-2 required for use on the reagent strip 7 and the sealing aluminum film on the top of the reagent hole.

[0055] Step 4: The reagent lateral movement drive component 3-6 and the liquid transfer lifting drive component 4-4 cooperate with each other, so that the liquid transfer gun 4-3 in the liquid transfer module 4 is connected to the TIP tip 8 on the tip box 3-5 and performs sample reaction processing.

[0056] The sample reaction process is as follows: adding enzyme label to the sample, adding magnetic beads and aspirating to mix the reaction, separating the magnetic beads, washing multiple times, and adding substrate. If the sample needs to be lysed, the sample is lysed once or multiple times before adding the enzyme label. The magnetic bead separation and multiple washings are performed in the same luminescent detection hole 7-2 located at the end of the reagent strip, and during the entire magnetic bead separation and washing process, the magnetic beads do not leave the luminescent detection hole 7-2.

[0057] In the above process, the method of sucking the waste liquid in the luminescence detection hole 7-2 during the magnetic bead separation and washing process is: using the Tip suction head 8 to transfer all the liquid in the luminescence detection hole 7-2 to the location where the waste liquid is stored under magnetic suction conditions.

[0058] The specific magnetic attraction conditions are: the magnetic structure 5-4 in the magnetic attraction module 5 is attached to the outer wall of the luminescent detection hole 7-2 of each reagent strip 7, so that the magnetic beads are adsorbed on the inner wall of the luminescent detection hole 7-2 to prevent the magnetic beads from being sucked away by the Tip suction head 8. This side wall adsorption method can keep the magnetic beads in the luminescent detection hole 7-2 throughout the magnetic bead separation and cleaning process without the need to be transferred with the Tip suction head 8 multiple times, thereby achieving thorough cleaning while significantly reducing the risk of magnetic bead loss; in addition, this process is also simpler than the existing magnetic bead separation and cleaning process.

[0059] In the above process, the specific process of the cleaning operation is: use the Tip pipette 8 to transfer the cleaning solution in the washing solution hole to the luminescence detection hole 7-2, and perform multiple suction mixing; then, use the Tip pipette 8 to transfer the cleaning solution in the luminescence detection hole 7-2 to the original washing solution hole under magnetic suction conditions.

[0060] Step 5: The reagent lateral drive assembly 3-6 drives the reagent carrying box 3-4 to move to the bottom of the detection module 6; the two-axis drive assembly 6-1 in the detection module 6 drives the photomultiplier tube 6-3 to pass over the luminescence detection hole 7-2 of each reagent strip 7 in turn and perform detection respectively to obtain the chemiluminescence characteristic detection result of the sample. During each detection, the fastener on the photomultiplier tube 6-3 is just pressed on the opening of the luminescence detection hole 7-2 for detection.

[0061] Step 6: After the test is completed, the pipette gun 4-3 of the pipetting module 4 releases the Tip 8 into the tip box 3-5, and the incubation module 3, the pipetting module 4, the magnetic module 5, and the detection module 6 are all reset; the staff takes away the used Tip 8 and reagent strip 7 at the exit of the whole machine.

Claims

1. A multi-channel sidewall adsorption magnetic separation detection system, comprising a base (1), and an incubation module (3), a pipetting module (4), a magnetic adsorption module (5) and a detection module (6) mounted on the base (1); the incubation module (3) is used to store and move a reagent strip; the pipetting module (4) is used to transfer reagents in different wells of the reagent strip; the detection module (6) is used to perform luminescence characteristic detection on a luminescence detection well (7-2) of the reagent strip; characterized in that: The magnetic attraction module (5) comprises a guide bracket (5-1), a magnetic attraction substrate (5-2), a magnetic attraction displacement drive component (5-3) and a magnetic structure (5-4); the magnetic attraction substrate (5-2) is connected to the guide bracket (5-1) and moves under the drive of the magnetic attraction displacement drive component (5-3); the magnetic structure (5-4) is installed on the magnetic attraction substrate (5-2); The magnetic attraction substrate (5-2) has two working positions, namely a magnetic attraction position and an avoidance position; when the position of the pipetting module (4) corresponds to the position of the luminescent detection hole (7-2) of the reagent strip, the magnetic structure (5-4) on the magnetic attraction substrate (5-2) in the magnetic attraction position generates a magnetic attraction force on the magnetic beads in the luminescent detection hole (7-2) so that the magnetic beads adhere to the wall.

2. A multi-channel sidewall adsorption magnetic separation detection system according to claim 1, characterized in that: During operation, the separation of the magnetic beads from the sample liquid and the cleaning of the magnetic beads are both performed in the luminescence detection hole (7-2); the process of separating the magnetic beads from the sample liquid and the cleaning liquid is as follows: first, the magnetic attraction substrate (5-2) is moved to the magnetic attraction position, so that the magnetic beads in the luminescence detection hole (7-2) are adsorbed on the inner wall of the luminescence detection hole (7-2) by the permanent magnet (5-4); then, the sample liquid or the cleaning liquid is sucked out of the luminescence detection hole (7-2) using the pipetting module (4).

3. A multi-channel sidewall adsorption magnetic separation detection system according to claim 1 or 2, characterized in that: When the magnetic attraction substrate (5-2) is in the avoidance position, the bottom surface of the magnetic attraction substrate (5-2) is higher than the top of the incubation module (3).

4. A multi-channel sidewall adsorption magnetic separation detection system according to claim 1 or 2, characterized in that: The incubation module (3) comprises a slide table (3-3), a reagent carrying box (3-4), a pipette tip box (3-5) and a reagent transverse movement drive assembly (3-6); the slide table (3-3) is slidably connected to the base and driven by the reagent transverse movement drive assembly (3-6); the reagent carrying box (3-4) and the pipette tip box (3-5) are both mounted on the slide table (3-3); one or more reagent strip mounting positions are provided on the reagent carrying box (3-4); each reagent strip mounting position has one or more corresponding pipette tip storage holes on the pipette tip box (3-5).

5. A multi-channel sidewall adsorption magnetic separation detection system according to claim 4, characterized in that: The reagent carrying box (3-4) is provided with a plurality of reagent strip mounting positions arranged side by side; in the reagent strip (7) mounted in the reagent strip mounting position, the luminescent detection hole (7-2) is located at the end of the reagent strip (7) farthest from the tip box (3-5); and the magnetic structure (5-4) on the magnetic attraction substrate (5-2) in the magnetic attraction position is aligned with each reagent strip (7) along the length direction of the reagent strip (7).

6. A multi-channel sidewall adsorption magnetic separation detection system according to claim 5, characterized in that: The number of the magnetic structures (5-4) on the magnetic substrate (5-2) and the reagent strip mounting positions on the reagent carrying box (3-4) are equal and correspond one to one; a groove structure matching the shape of the outer wall of the luminescent detection hole (7-2) is provided on the outer surface of the magnetic structure (5-4).

7. A multi-channel sidewall adsorption magnetic separation detection system according to claim 4, characterized in that: The incubation module (3) further comprises an incubation guide rail (3-1) and a guide rod (3-2); the incubation guide rail (3-1) and the guide rod (3-2) are parallel to each other and are fixed on the base (1); and the two sides of the bottom of the slide (3-3) are respectively slidably connected to the incubation guide rail (3-1) and the guide rod (3-2).

8. A multi-channel sidewall adsorption magnetic separation detection system according to claim 2, characterized in that: A heating component is installed inside the reagent carrying box (3-4) or on the slide (3-3).

9. The multi-channel sidewall adsorption magnetic separation detection system according to claim 1, characterized in that: The magnetic structure (5-4) adopts a permanent magnet or an electromagnet.

10. The multi-channel sidewall adsorption magnetic separation detection system according to claim 1, characterized in that: The pipetting module (4) comprises a pipetting support (4-1), a lifting slide (4-2), a pipetting gun (4-3), a membrane breaking head and a pipetting lifting drive assembly (4-4); the pipetting support (4-1) is fixed on a support frame (2); the lifting slide (4-2) is slidably connected to the pipetting support (4-1) and driven by the pipetting lifting drive assembly (4-4); the lifting slide (4-2) is mounted with a pipetting gun (4-3) and a membrane breaking head corresponding to the reagent strip mounting position.

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