Human papilloma virus nucleic acid detection kit based on RPC-fluorescent probe method and detection method thereof

Abstract

The invention relates to a human papilloma virus nucleic acid detection kit based on an RPC-fluorescent probe method and a detection method thereof.The human papilloma virus nucleic acid detection kit comprises a kit body, a liquid adding opening and an observation window, the liquid adding opening and the observation window are formed in the kit body, a PVC bottom plate is arranged in the kit body, and a detection film, a gold pad and water absorption filter paper are arranged on the PVC bottom plate; one end of the gold pad and one end of the water absorption filter paper are attached to the two ends of the detection film respectively, and the gold pad corresponds to the observation window in position. The sample pad and the gold pad are isolated through the arranged partition plate, so that liquid entering the box body by mistake can only wet the sample pad and cannot pass through the gold pad, and the detection film is wetted; the sample pad can be continuously and normally used after being dried, so that the protectiveness of the detection film is improved, the economic loss caused by the fact that the detection film is abandoned is reduced, the liquid adding opening can be sealed through the arranged sealing assembly, liquid is prevented from accidentally entering the box body to wet the sample pad, and the dual-guarantee effect is achieved.

Description

Technical Field

[0001] The invention relates to a human papillomavirus nucleic acid detection kit based on an RPC-fluorescent probe method and a detection method thereof. Background Art

[0002] Human papillomavirus (HPV) is a virus that infects the human epidermis and mucous membranes. It is widely distributed in humans and animals and has a high host specificity. Only humans can be infected with HPV, which can cause proliferation of the squamous epithelium of the human skin and mucous membranes, leading to a variety of skin diseases and is highly correlated with the occurrence of some cancers.

[0003] Cervical cancer caused by human papillomavirus infection is mainly because the virus replicates in cells and continuously stimulates the body's immune system, leading to abnormal proliferation of local tissues. This process may take several years and is related to the individual's immune status. After infection with human papillomavirus, symptoms such as increased vaginal discharge and abnormal bleeding may occur. These symptoms are usually caused by direct damage to cervical epithelial cells by the virus and the induced inflammatory response.

[0004] The fluorescent PCR method for human papillomavirus nucleic acid detection can be tested by collecting cervical exfoliated cells, or by scraping samples from the surface of the cervix with a special brush. In addition, acetic acid white test, colposcopy and other methods can be used to assist diagnosis. The treatment of human papillomavirus infection needs to be formulated according to the specific situation. For example, antiviral treatment with drugs such as interferon α can be used according to the doctor's advice. For those with lesions, surgical removal of the lesion tissue should be considered if necessary.

[0005] Although it is relatively convenient to use nucleic acid detection kits to detect human papillomavirus at present, there are some disadvantages in the use of nucleic acid detection kits. For example, the sample pad, gold pad, detection membrane, and absorbent filter paper inside the existing nucleic acid detection kit are all connected in an overlapping manner. During normal use, after the sample contacts the sample pad, it will be immediately transferred in sequence, so that the detection membrane can be observed to determine whether it is infected with human papillomavirus; however, since it is generally necessary to wash your hands before operation, water stains or other liquids may accidentally enter the inside of the kit from the liquid filling port during use, causing the sample pad to be wetted, and then the gold pad, detection membrane, and absorbent filter paper are moistened in turn. The moistened detection membrane cannot be used normally, resulting in the scrapping of the nucleic acid detection kit and economic losses. Summary of the invention

[0006] The object of the present invention is to provide a human papillomavirus nucleic acid detection kit and a detection method thereof based on the RPC-fluorescent probe method, so as to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions: A human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method comprises a box body, a liquid filling port and an observation window respectively arranged on the box body, a PVC bottom plate is arranged inside the box body, a detection membrane, a gold pad and a water-absorbing filter paper are respectively arranged on the PVC bottom plate, one end of the gold pad and the water-absorbing filter paper are respectively attached to the two ends of the detection membrane, and the position of the gold pad corresponds to that of the observation window; A movable plate is horizontally slidably disposed inside the box body, a sample pad is disposed on the movable plate, and the sample pad corresponds to the position of the liquid adding port; A partition is vertically slidably arranged inside the box body, and the partition is located between the gold pad and the sample pad. A sliding column is vertically movably arranged on the box body, and a linkage component is arranged between the sliding column and the partition. When the sliding column moves vertically downward, the sliding column will drive the partition to move vertically upward through the linkage component, thereby releasing the isolation between the gold pad and the sample pad; A transmission structure is provided between the partition and the moving plate. During the upward movement of the partition, the moving plate drives the sample pad to fit the gold pad under the action of the transmission structure. The liquid filling port is provided with a sealing component, and the sealing component is connected to the partition. During the vertical upward movement of the partition, the sealing component will follow the movement of the partition and switch the liquid filling port from a sealed state to an open state.

[0008] As a further solution of the present invention: the linkage assembly comprises a vertical rod vertically arranged on the partition and a first swivel sleeved on the sliding column, and the outer wall of the vertical rod is sleeved with a second swivel; The first rotating ring and the second rotating ring are connected by a plurality of connecting columns, a bracket is arranged inside the box body, and the plurality of connecting columns are rotatably matched with the bracket.

[0009] As a further solution of the present invention: the linkage assembly further comprises a first ball rollingly engaged in the first rotating ring and a second ball rollingly engaged in the second rotating ring; The outer wall of the sliding column is provided with a No. 1 spiral groove, and the outer wall of the vertical rod is provided with a No. 2 spiral groove. The No. 1 ball and the No. 2 ball are also rollingly embedded in the No. 1 spiral groove and the No. 2 spiral groove respectively.

[0010] As a further solution of the present invention: the transmission structure includes a U-shaped frame arranged on the partition and a support column arranged on the movable plate, and a connecting rod is rotatably arranged between the U-shaped frame and the support column.

[0011] As a further solution of the present invention: a first slider is provided at the bottom of the movable plate, and second sliders are provided at both ends of the partition respectively; The inner bottom of the box body is horizontally provided with a No. 1 slide groove, the inner side wall of the box body is vertically provided with a No. 2 slide groove, the No. 1 slider and the No. 2 slider are respectively located inside the No. 1 slide groove and the No. 2 slide groove and slide in cooperation with each other.

[0012] As a further solution of the present invention: the sealing assembly comprises a conduit with one end sealed and the other end fixedly connected to the liquid filling port, and a sealing ring is fixedly sleeved on the outer wall of the conduit; The side wall of the conduit is provided with a No. 1 through hole, the sealing ring is provided with a No. 2 through hole corresponding to the No. 1 through hole, the outer wall of the sealing ring is movably sleeved with a ring, the ring covers the No. 2 through hole, the outer wall of the ring is provided with an L-shaped rod, one end of the L-shaped rod is connected to the partition.

[0013] As a further solution of the present invention: the side wall of the slide column is provided with a guide block, the interior of the box body is vertically provided with a guide groove, and the guide block is located inside the guide groove and slides with each other; A No. 1 spring is arranged inside the guide groove, and two ends of the No. 1 spring are respectively against the bottom of the guide block and the bottom surface of the guide groove.

[0014] As a further solution of the present invention: a locking structure is provided between the box body and the sliding column, and the locking structure comprises a sleeve and a sliding rod slidably matched with the sleeve; A groove is horizontally provided on the side wall of the sliding column, the sleeve and the sliding rod are both located inside the groove, and one end of the sleeve is connected to the inner wall of the groove, a triangular stop block is provided at one end of the sliding column, and a triangular stop groove matching the triangular stop block is provided inside the box body; A No. 2 spring is arranged inside the sleeve, and two ends of the No. 2 spring are respectively against the inner side wall of the sleeve and the other end of the sliding column.

[0015] A detection method of the human papillomavirus nucleic acid detection kit as described above comprises the following steps: Step 1: The operator should disinfect and dry both hands to ensure that they are clean and dry to prevent bacteria carried by the hands from affecting the test. Step 2: Place the box body horizontally on the operating table, and then use a tool to drive the slide column to move vertically downward. During this process, the partition will move vertically upward to release the isolation of the sample pad and the gold pad, and the moving plate will drive the sample pad and the gold pad to fit each other. At the same time, the liquid filling port will switch from a sealed state to an open state; Step 3: When the slide column moves down to a certain extent, the slide column will be locked with the box body under the action of the locking structure, so as to prevent the slide column from returning to the vertical position under the action of the No. 1 spring after the external force is removed; Step 4: With the help of tools, inject the sample and auxiliary detection reagents into the box through the liquid filling port and contact them with the sample pad. Then, under the action of absorbent filter paper, the sample and auxiliary detection reagents are transferred to the detection membrane through the gold pad. Finally, observe the phenomenon of the mutual reaction between the sample, auxiliary detection reagents and detection membrane to determine whether the patient is infected.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The sample pad and the gold pad are isolated by the partition plate, so that the liquid that enters the box body by mistake can only wet the sample pad but cannot pass through the gold pad to wet the detection membrane. After the sample pad is dried, it can continue to be used normally, which improves the protection of the detection membrane and reduces the economic loss caused by the detection membrane being scrapped. The sealing component can seal the liquid filling port to prevent liquid from accidentally entering the box and wetting the sample pad, thus playing a double protection role; When in use, the partition can release the isolation between the sample pad and the gold pad through the cooperation between the various structures, and the liquid filling port is switched to an open state. At the same time, the sample pad will approach the gold pad and fit each other. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A schematic diagram of the overall structure of an embodiment of a human papillomavirus nucleic acid detection kit.

[0018] Figure 2 A half-section view of a kit body according to an embodiment of a kit for detecting human papillomavirus nucleic acid.

[0019] Figure 3 for Figure 2 Enlarged view of point B in the middle.

[0020] Figure 4 A partial structural cross-sectional view of an embodiment of a human papillomavirus nucleic acid detection kit.

[0021] Figure 5 for Figure 4 Enlarged view of point A in the middle.

[0022] Figure 6 A cross-sectional view of a box body, a sliding column, a bracket, a catheter, a sealing ring, and a collar structure in one embodiment of a human papillomavirus nucleic acid detection kit.

[0023] Figure 7 for Figure 6 Enlarged view of center C.

[0024] Figure 8 for Figure 7 Enlarged view of point D in the middle.

[0025] Fig. 9 for Figure 6 Enlarged view of point E in the middle.

[0026] Fig.10 A disassembled diagram of a box body, a movable plate, and a partition in one embodiment of a human papillomavirus nucleic acid detection kit.

[0027] Fig.11 for Fig.10 Enlarged view of point F in the middle.

[0028] Fig.12 An exploded view of the linkage components in one embodiment of a human papillomavirus nucleic acid detection kit.

[0029] Fig.13 An exploded view of the locking structure in one embodiment of a human papillomavirus nucleic acid detection kit.

[0030] In the figure: 1, box body; 101, slide slot No. 1; 102, slide slot No. 2; 2, liquid filling port; 3, observation window; 4, PVC bottom plate; 5, detection membrane; 6, gold pad; 7, absorbent filter paper; 8, moving plate; 801, slider No. 1; 9, sample pad; 10, partition; 1001, slider No. 2; 11, slide column; 1101, spiral groove No. 1; 12, vertical rod; 1201, spiral groove No. 2; 13, first rotating ring; 14, second rotating ring; 15, connecting rod Connecting column; 16, bracket; 17, ball bearing No. 1; 18, ball bearing No. 2; 19, U-shaped frame; 20, pillar; 21, connecting rod; 22, guide tube; 2201, through hole No. 1; 23, sealing ring; 2301, through hole No. 2; 24, collar; 25, L-shaped rod; 26, guide block; 27, guide groove; 28, spring No. 1; 29, sleeve; 30, sliding rod; 31, groove; 32, triangular limit block; 33, spring No. 2; 34, triangular limit groove. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] In addition, when an element in the present invention is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only embodiment.

[0033] See also Figures 1 to 13 In an embodiment of the present invention, a human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method comprises a box body 1 and a liquid filling port 2 and an observation window 3 respectively arranged on the box body 1, a PVC bottom plate 4 is arranged inside the box body 1, and a detection membrane 5, a gold pad 6 and a water-absorbing filter paper 7 are respectively arranged on the PVC bottom plate 4, one end of the gold pad 6 and the water-absorbing filter paper 7 are respectively attached to the two ends of the detection membrane 5, and the gold pad 6 corresponds to the position of the observation window 3; A movable plate 8 is horizontally slidably disposed inside the box body 1, and a sample pad 9 is disposed on the movable plate 8, and the sample pad 9 corresponds to the position of the liquid adding port 2; A partition 10 is vertically slidably provided inside the box body 1, and the partition 10 is located between the gold pad 6 and the sample pad 9. A slide post 11 is vertically movably provided on the box body 1, and a linkage component is provided between the slide post 11 and the partition 10. When the slide post 11 moves vertically downward, the slide post 11 will drive the partition 10 to move vertically upward through the linkage component, thereby releasing the isolation between the gold pad 6 and the sample pad 9; A transmission structure is provided between the partition 10 and the moving plate 8. During the upward movement of the partition 10, the moving plate 8 drives the sample pad 9 to fit with the gold pad 6 under the action of the transmission structure. The liquid filling port 2 is provided with a sealing component, and the sealing component is connected to the partition 10. During the vertical upward movement of the partition 10, the sealing component will follow the movement of the partition 10 and switch the liquid filling port 2 from a sealed state to an open state.

[0034] In this solution, the sample pad 9 and the gold pad 6 are isolated by the partition 10, so that the liquid that mistakenly enters the box body 1 can only wet the sample pad 9 but cannot pass through the gold pad 6 to wet the detection film 5. After the sample pad 9 is dried, it can continue to be used normally, which improves the protection of the detection film 5 and reduces the economic loss caused by the detection film 5 being scrapped. The sealing component can seal the liquid filling port 2 to prevent the liquid from accidentally entering the box body 1 and wetting the sample pad 9, thus playing a double protection role; When the slide column 11 is pushed downward vertically by external force, the slide column 11 will drive the partition 10 to move vertically upward through the linkage assembly, thereby releasing the isolation between the gold pad 6 and the sample pad 9, and when the partition 10 moves upward, the sealing assembly will follow the partition 10 and switch the liquid filling port 2 from a sealed state to an open state; In addition, during the process of the partition 10 moving vertically upward, the partition 10 will drive the sample pad 9 to fit with the gold pad 6 through the transmission structure, and the detection operation can be carried out.

[0035] See also Figure 5 , Figure 7 , Fig.12 The linkage assembly includes a vertical rod 12 vertically arranged on the partition 10 and a first swivel 13 sleeved on the sliding column 11, and the outer wall of the vertical rod 12 is sleeved with a second swivel 14; The first rotating ring 13 and the second rotating ring 14 are connected by a plurality of connecting posts 15 . A bracket 16 is disposed inside the box body 1 . The plurality of connecting posts 15 are rotatably matched with the bracket 16 .

[0036] In this embodiment, since the plurality of connecting posts 15 are rotatably matched with the bracket 16 and the first rotating ring 13 and the second rotating ring 14 are respectively connected to the plurality of connecting posts 15 , the first rotating ring 13 and the second rotating ring 14 are rotatably arranged in the box body 1 .

[0037] See also Figure 5 , Fig.12 The linkage assembly further includes a first ball 17 rollingly engaged in the first rotating ring 13 and a second ball 18 rollingly engaged in the second rotating ring 14; The outer wall of the sliding column 11 is provided with a No. 1 spiral groove 1101, and the outer wall of the vertical rod 12 is provided with a No. 2 spiral groove 1201. The No. 1 ball 17 and the No. 2 ball 18 are also rollingly embedded in the No. 1 spiral groove 1101 and the No. 2 spiral groove 1201 respectively.

[0038] In this embodiment, since the No. 1 ball 17 rollingly engaged in the slide post 11 is also rollingly engaged in the No. 1 spiral groove 1101, when the slide post 11 moves vertically downward and cannot rotate, the first rotating ring 13 will rotate, so that the second rotating ring 14 rotates with the first rotating ring 13 through the plurality of connecting posts 15; Because the No. 2 ball 18 rollingly engaged in the vertical rod 12 is also rollingly engaged in the No. 2 spiral groove 1201, and the vertical rod 12 is connected to the partition 10, and the partition 10 is vertically slidably arranged inside the box body 1, therefore, during the rotation of the second rotating ring 14, the vertical rod 12 cannot rotate, thereby driving the partition 10 to move vertically upward, thereby releasing the isolation of the gold pad 6 and the sample pad 9.

[0039] See also Figure 3 The transmission structure includes a U-shaped frame 19 arranged on the partition 10 and a support 20 arranged on the movable plate 8, and a connecting rod 21 is rotatably arranged between the U-shaped frame 19 and the support 20.

[0040] In this embodiment, since the connecting rod 21 is rotatably connected to the support 20 on the movable plate 8 and the U-shaped frame 19 on the partition 10, during the vertical upward movement of the partition 10, the movable plate 8 will be driven horizontally toward the gold pad 6 through the connecting rod 21, so that the sample pad 9 and the gold pad 6 are fitted with each other.

[0041] See also Fig.11 , a first slider 801 is provided at the bottom of the movable plate 8, and a second slider 1001 is provided at both ends of the partition plate 10; A first slide groove 101 is horizontally opened at the inner bottom of the box body 1, and a second slide groove 102 is vertically opened on the inner side wall of the box body 1. The first slider 801 and the second slider 1001 are respectively located inside the first slide groove 101 and the second slide groove 102 and slide in cooperation with each other.

[0042] In this embodiment, since the first slide block 801 is located inside the first slide groove 101 and slides with each other, the movable plate 8 can only slide horizontally in the box body 1; Furthermore, because the second sliding block 1001 is located inside the second sliding groove 102 and slides with each other, the partition plate 10 can only slide vertically in the box body 1.

[0043] See also Figure 4 , Fig. 9 The sealing assembly includes a conduit 22 with one end sealed and the other end fixedly connected to the liquid filling port 2, and a sealing ring 23 is fixedly sleeved on the outer wall of the conduit 22; The side wall of the conduit 22 is provided with a No. 1 through hole 2201, the sealing ring 23 is provided with a No. 2 through hole 2301 corresponding to the No. 1 through hole 2201, the outer wall of the sealing ring 23 is movably provided with a ring 24, the ring 24 covers the No. 2 through hole 2301, the outer wall of the ring 24 is provided with an L-shaped rod 25, one end of the L-shaped rod 25 is connected to the partition 10.

[0044] In this embodiment, since the collar 24 covers the second through hole 2301, the conduit 22 is in a sealed state, and the liquid entering the conduit 22 through the liquid filling port 2 cannot enter the box body 1; Since the ring 24 is movably mounted on the outer wall of the sealing ring 23 and the ring 24 is connected to the partition 10 through the L-shaped rod 25, during the vertical upward movement of the partition 10, the ring 24 will be driven by the L-shaped rod 25 to slide on the outer wall of the sealing ring 23, so that the No. 2 through hole 2301 is exposed from the ring 24, that is, the No. 1 through hole 2201 on the conduit 22 is in an open state.

[0045] See also Figure 5 , Figure 7 The side wall of the slide column 11 is provided with a guide block 26, and a guide groove 27 is vertically opened inside the box body 1. The guide block 26 is located inside the guide groove 27 and slides with each other; A No. 1 spring 28 is disposed inside the guide groove 27 , and two ends of the No. 1 spring 28 abut against the bottom of the guide block 26 and the bottom surface of the guide groove 27 , respectively.

[0046] In this embodiment, since the guide block 26 is located inside the guide groove 27 and slides with each other, the slide post 11 can only slide with the box body 1 vertically and cannot rotate. Because of the existence of spring No. 1 28 and its maintained compressed state, in the absence of external force, spring No. 1 28 will make the slide column 11 always have a tendency to move vertically upward through the guide block 26, so that the partition 10 is always located between the sample pad 9 and the gold pad 6.

[0047] See also Figure 8 , Fig.13 A locking structure is provided between the box body 1 and the slide post 11, and the locking structure includes a sleeve 29 and a slide rod 30 that slides with the sleeve 29; A groove 31 is horizontally provided on the side wall of the sliding column 11, the sleeve 29 and the sliding rod 30 are both located inside the groove 31, and one end of the sleeve 29 is connected to the inner wall of the groove 31, a triangular stop block 32 is provided at one end of the sliding column 11, and a triangular stop groove 34 adapted to the triangular stop block 32 is provided inside the box body 1; A No. 2 spring 33 is disposed inside the sleeve 29 , and two ends of the No. 2 spring 33 abut against the inner side wall of the sleeve 29 and the other end of the sliding column 11 , respectively.

[0048] In this embodiment, due to the presence of the No. 2 spring 33 and the maintained compressed state, the No. 2 spring 33 will make the slide bar 30 always have a tendency to extend outward from the sleeve 29, so that the triangular limit block 32 is always in close contact with the box body 1; During the vertical downward movement of the sliding column 11, the groove 31 will drive the sleeve 29, the sliding rod 30 and the triangular limit block 32 to move vertically downward synchronously. When the triangular limit block 32 corresponds to the position of the triangular limit groove 34, due to the action of the No. 2 spring 33, the triangular limit block 32 will extend into the triangular limit groove 34 and lock with each other, that is, the sliding column 11 and the box body 1 are locked with each other, thereby preventing the sliding column 11 from returning to the vertical upward position under the action of the No. 1 spring 28 after the external force is removed.

[0049] A detection method of the human papillomavirus nucleic acid detection kit as described above comprises the following steps: Step 1: The operator disinfects and dries both hands first to ensure that the hands are clean and dry to prevent bacteria carried by the hands from affecting the test; Step 2: Place the box body horizontally on the operating table, and then use a tool to drive the slide column to move vertically downward. During this process, the partition will move vertically upward to release the isolation of the sample pad and the gold pad, and the moving plate will drive the sample pad and the gold pad to fit each other. At the same time, the liquid filling port will switch from a sealed state to an open state; Step 3: When the slide column moves down to a certain extent, the slide column will be locked with the box body under the action of the locking structure, so as to prevent the slide column from returning to the vertical position under the action of the No. 1 spring after the external force is removed; Step 4: With the help of tools, inject the sample and auxiliary detection reagents into the box through the liquid filling port and contact them with the sample pad. Then, under the action of absorbent filter paper, the sample and auxiliary detection reagents are transferred to the detection membrane through the gold pad. Finally, observe the phenomenon of the mutual reaction between the sample, auxiliary detection reagents and detection membrane to determine whether the patient is infected.

[0050] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0051] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method, comprising a box body (1) and a liquid filling port (2) and an observation window (3) respectively arranged on the box body (1), characterized in that: A PVC bottom plate (4) is arranged inside the box body (1), and a detection membrane (5), a gold pad (6) and a water-absorbing filter paper (7) are arranged on the PVC bottom plate (4), respectively. One end of the gold pad (6) and the water-absorbing filter paper (7) are respectively attached to two ends of the detection membrane (5), and the position of the gold pad (6) corresponds to that of the observation window (3); A movable plate (8) is horizontally slidably disposed inside the box body (1), a sample pad (9) is disposed on the movable plate (8), and the sample pad (9) corresponds to the position of the liquid filling port (2); A partition (10) is vertically slidably arranged inside the box body (1), and the partition (10) is located between the gold pad (6) and the sample pad (9). A sliding column (11) is vertically movably arranged on the box body (1), and a linkage component is arranged between the sliding column (11) and the partition (10). When the sliding column (11) moves vertically downward, the sliding column (11) drives the partition (10) to move vertically upward through the linkage component, thereby releasing the isolation between the gold pad (6) and the sample pad (9); A transmission structure is provided between the partition (10) and the movable plate (8), and in the process of the partition (10) moving upward, the movable plate (8) will drive the sample pad (9) to fit with the gold pad (6) under the action of the transmission structure; The liquid filling port (2) is provided with a sealing component, the sealing component being connected to the partition (10), and when the partition (10) moves vertically upward, the sealing component will follow the movement of the partition (10) and switch the liquid filling port (2) from a sealed state to an open state.

2. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 1, characterized in that: The linkage assembly comprises a vertical rod (12) vertically arranged on the partition (10) and a first swivel (13) sleeved on the sliding column (11); a second swivel (14) is sleeved on the outer wall of the vertical rod (12); The first rotating ring (13) and the second rotating ring (14) are connected via a plurality of connecting columns (15); a bracket (16) is provided inside the box body (1); and the plurality of connecting columns (15) are rotatably matched with the bracket (16).

3. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 2, characterized in that: The linkage assembly further comprises a first ball (17) rollingly engaged inside the first rotating ring (13) and a second ball (18) rollingly engaged inside the second rotating ring (14); The outer wall of the sliding column (11) is provided with a first spiral groove (1101), and the outer wall of the vertical rod (12) is provided with a second spiral groove (1201). The first ball (17) and the second ball (18) are also rollingly engaged in the first spiral groove (1101) and the second spiral groove (1201), respectively.

4. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 3, characterized in that: The transmission structure comprises a U-shaped frame (19) arranged on the partition (10) and a support column (20) arranged on the movable plate (8), and a connecting rod (21) is rotatably arranged between the U-shaped frame (19) and the support column (20).

5. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 4, characterized in that: A first sliding block (801) is provided at the bottom of the movable plate (8), and second sliding blocks (1001) are provided at both ends of the partition plate (10); The inner bottom of the box body (1) is horizontally provided with a first slide groove (101), the inner side wall of the box body (1) is vertically provided with a second slide groove (102), and the first slide block (801) and the second slide block (1001) are respectively located inside the first slide groove (101) and the second slide groove (102) and slide in cooperation with each other.

6. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 5, characterized in that: The sealing assembly comprises a conduit (22) with one end sealed and the other end fixedly connected to the liquid filling port (2), and a sealing ring (23) is fixedly sleeved on the outer wall of the conduit (22); The side wall of the conduit (22) is provided with a first through hole (2201), the sealing ring (23) is provided with a second through hole (2301) corresponding to the first through hole (2201), the outer wall of the sealing ring (23) is movably sleeved with a collar (24), the collar (24) covers the second through hole (2301), the outer wall of the collar (24) is provided with an L-shaped rod (25), one end of the L-shaped rod (25) is connected to the partition (10).

7. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 6, characterized in that: A guide block (26) is provided on the side wall of the slide column (11); a guide groove (27) is vertically opened inside the box body (1); the guide block (26) is located inside the guide groove (27) and slides with each other; A No. 1 spring (28) is arranged inside the guide groove (27), and two ends of the No. 1 spring (28) respectively abut against the bottom of the guide block (26) and the bottom surface of the guide groove (27).

8. The human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method according to claim 7, characterized in that: A locking structure is provided between the box body (1) and the sliding column (11), the locking structure comprising a sleeve (29) and a sliding rod (30) slidably matched with the sleeve (29); A groove (31) is horizontally formed on the side wall of the slide column (11); the sleeve (29) and the slide rod (30) are both located inside the groove (31) and one end of the sleeve (29) is connected to the inner wall of the groove (31); a triangular stop block (32) is provided at one end of the slide column (11); and a triangular stop groove (34) adapted to the triangular stop block (32) is formed inside the box body (1); A No. 2 spring (33) is disposed inside the sleeve (29), and two ends of the No. 2 spring (33) respectively abut against the inner side wall of the sleeve (29) and the other end of the sliding column (11).

9. A detection method of a human papillomavirus nucleic acid detection kit based on the RPC-fluorescent probe method as claimed in claim 8, characterized in that: The following steps are involved: Step 1: The operator disinfects and dries both hands first to ensure that the hands are clean and dry to prevent bacteria carried by the hands from affecting the test; Step 2: Place the box body horizontally on the operating table, and then use a tool to drive the slide column to move vertically downward. During this process, the partition will move vertically upward to release the isolation of the sample pad and the gold pad, and the moving plate will drive the sample pad and the gold pad to fit each other. At the same time, the liquid filling port will switch from a sealed state to an open state; Step 3: When the slide column moves down to a certain extent, the slide column will be locked with the box body under the action of the locking structure, so as to prevent the slide column from returning to the vertical position under the action of the No. 1 spring after the external force is removed; Step 4: With the help of tools, inject the sample and auxiliary detection reagents into the box through the liquid filling port and contact them with the sample pad. Then, under the action of absorbent filter paper, the sample and auxiliary detection reagents are transferred to the detection membrane through the gold pad. Finally, observe the phenomenon of the mutual reaction between the sample, auxiliary detection reagents and detection membrane to determine whether the patient is infected.

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