Cell culture instrument and cell culture method

Abstract

The invention discloses a cell culture instrument and a cell culture method, and relates to the technical field of cell culture.The cell culture instrument comprises a box body, and a plurality of stations are arranged in the box body in the circumferential direction; the plurality of incubators are detachably arranged on the stations respectively; one end of the flow channel penetrates through the incubator, and the other end of the flow channel is communicated with the culture chamber; a one-way valve is arranged at the runner; the plurality of pistons are respectively arranged in the flow channels; the cell culture method comprises the following steps: placing a culture solution and cells to be cultured in a culture device, and adding the culture solution into a flow channel; when a new culture solution needs to be added into the incubator, the piston moves downwards, and the culture solution in the flow channel is added into the incubator; when the culture solution in the flow channel is consumed to a preset value, the piston moves upwards and is opened, and after a new culture solution is added into the flow channel, the piston is closed. According to the scheme, the incubator does not need to be taken out of the box body when a new culture solution is added, so that cells are prevented from being polluted, and the operation is simple and convenient.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a cell culture apparatus and a cell culture method. Background Art

[0002] In cell culture, cells consume nutrients in the culture medium during growth and division, such as amino acids, vitamins, sugars, etc. Over time, these nutrients will gradually become depleted, so new culture medium needs to be added regularly to provide the necessary nutrients.

[0003] At present, in a closed culture environment, there are usually two ways to add new culture medium. One is to directly take the culture dish out of the incubator, which is easy to cause cell contamination in the culture dish. The other is to place the incubator in a sterile box, wear sterile gloves, then take the culture dish out of the incubator and put it in the sterile box to add new culture medium, which is more troublesome. Summary of the invention

[0004] The purpose of the present invention is to provide a cell culture apparatus and a cell culture method, which do not require manual addition of culture fluid and can also prevent cells from being contaminated.

[0005] The present invention is achieved through the following technical solutions:

[0006] In one aspect, the present invention provides a cell culture apparatus, comprising:

[0007] The box body has a plurality of workstations arranged inside along the circumferential direction;

[0008] A plurality of culture vessels are detachably arranged on the workstations; the culture vessels have a culture chamber, and the culture vessels also have a flow channel, one end of the flow channel passes through the culture vessel, and the other end is connected to the culture chamber; a one-way valve is provided at the flow channel, so that the flow channel is connected to the culture chamber in one direction;

[0009] A plurality of pistons are respectively arranged in the flow channel; the pistons can be controlled to open and close, and can also be controlled to slide along the flow channel.

[0010] In a further technical solution, an adding mechanism is also included, and the adding mechanism includes a rotating plate, a mounting plate, a first connecting rod, a second connecting rod, a third connecting rod, a fourth connecting rod, a first telescopic rod and a pipette gun;

[0011] The rotating plate is rotatably arranged on the inner top of the box body; the mounting plate is arranged on the bottom of the rotating plate;

[0012] One end of the first connecting rod is rotatably connected to the mounting plate, the middle portion of the third connecting rod is rotatably connected to the mounting plate, one end of the second connecting rod is rotatably connected to the other end of the first connecting rod, and the other end of the second connecting rod is rotatably connected to one end of the third connecting rod;

[0013] The other end of the third connecting rod is rotatably connected to the middle part of the fourth connecting rod, one end of the fourth connecting rod is slidably connected to the rotating plate, and the other end is connected to the pipette gun;

[0014] Two ends of the first telescopic rod are rotatably connected to the rotating plate and the first connecting rod respectively.

[0015] In a further technical solution, the connecting plate is provided with a first arc groove and a second arc groove; the end of the second connecting rod is embedded in the first arc groove, can move along the first arc groove, and is rotatably connected with the end of the third connecting rod;

[0016] The end of the fourth connecting rod is embedded in the second arc groove and can move along the second arc groove.

[0017] In a further technical solution, the adding mechanism further includes a driving part, and the driving part includes a gear ring, a gear and a motor;

[0018] The gear ring is sleeved on the rotating plate, and the gear is rotatably disposed in the box body and meshes with the gear ring;

[0019] The motor is used to drive the gear to rotate.

[0020] In a further technical solution, the piston includes a mounting ring, a rotating disk and a plurality of closing plates;

[0021] The mounting ring is slidably disposed in the flow channel, the rotating plate is rotatably disposed in the mounting ring, and a plurality of closing plates are slidably disposed on the rotating plate and move inward or outward as the rotating plate rotates;

[0022] A slide groove is provided in the flow channel, and the slide groove has a straight structure and a spiral structure, and the spiral structure is located above the straight structure; the side wall of the mounting ring has a through groove, and the rotating disk has a protrusion, and the protrusion passes through the through groove and is embedded in the slide groove and can slide along the slide groove.

[0023] In a further technical solution, it further includes a plurality of second telescopic rods, wherein the plurality of second telescopic rods are arranged in the box body through brackets;

[0024] When the second telescopic rod is extended, the free end may abut against the piston.

[0025] In a further technical solution, a first magnet is provided on the first connecting rod, a second magnet is provided on the mounting ring, and the first magnet and the second magnet attract each other.

[0026] In a further technical solution, a plurality of third arc-shaped grooves are provided on the rotating disk, one end of the closing plate is embedded in the third arc-shaped groove, and can move along the third arc-shaped groove.

[0027] In a further technical solution, the one-way valve includes an elastic member, a valve port and a valve core;

[0028] The valve port is arranged in the flow channel, the elastic member is clamped in the flow channel and connected to the valve core, and the elastic member makes the valve core abut against the valve port;

[0029] Wherein, when the liquid in the flow channel reaches a maximum value, the pressure applied by the culture fluid to the valve core is less than the pressure applied by the elastic member to the valve core.

[0030] On the other hand, the present invention also provides a cell culture method, which is applied to the above-mentioned cell culture apparatus, comprising the following steps:

[0031] placing culture medium and cells to be cultured in a culture vessel, and adding culture medium into the flow channel;

[0032] When new culture fluid needs to be added to the culture vessel, the piston moves downward to add the culture fluid in the flow channel to the culture vessel;

[0033] When the culture fluid in the flow channel is consumed to a preset value, the piston moves upward and opens, and after new culture fluid is added to the flow channel, the piston closes.

[0034] The technical solution of the embodiment of the present invention has at least the following advantages and beneficial effects:

[0035] The cell culture apparatus of the present invention places culture fluid and cells to be cultured in a culture vessel, and adds culture fluid to the flow channel; when new culture fluid needs to be added to the culture vessel, the piston moves downward to add the culture fluid in the flow channel to the culture vessel; when the culture fluid in the flow channel is consumed to a preset value, the piston moves upward and opens, and after adding new culture fluid to the flow channel, the piston closes. Through the above scheme, the present invention does not need to remove the culture vessel from the box when adding new culture fluid, thereby avoiding cell contamination, and at the same time, adding new culture fluid does not require manual operation, which is simple and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a structural schematic diagram of the present invention;

[0037] Figure 2 is a cross-sectional view of the incubator;

[0038] Figure 3 for Figure 2 The enlarged view of point A in the middle;

[0039] Figure 4 is a schematic diagram of the structure of the incubator;

[0040] Figure 5 It is a structural schematic diagram of the piston;

[0041] Figure 6 It is a schematic diagram of the structure of the mounting ring;

[0042] Figure 7 It is a schematic diagram of the connection relationship between several closing plates and rotating plates;

[0043] Figure 8 To add a structural diagram of the mechanism Figure 1 ;

[0044] Fig. 9 To add a structural diagram of the mechanism Figure 2 .

[0045] Icon: 1-box, 2-culture vessel, 2a-culture chamber, 2b-flow channel, 2c-slide, 3-piston, 31-mounting ring, 31a-through groove, 32-rotating disk, 32a-third arc groove, 321-protrusion, 33-closing plate, 4-adding mechanism, 401-rotating plate, 402-mounting plate, 402a-first arc groove, 402b-second arc groove, 403-first connecting rod, 404-second connecting rod, 405-third connecting rod, 406-fourth connecting rod, 407-pipette, 408-first telescopic rod, 409-gear ring, 410-gear, 411-motor, 5-second telescopic rod, 6-bracket, 7-check valve, 71-valve core, 72-valve port, 73-elastic part. DETAILED DESCRIPTION

[0046] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0047] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] It should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as importance. They are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations.

[0049] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0050] In the description of the present invention, “plurality” means two or more than two, unless otherwise clearly and specifically defined.

[0051] Please refer to Figure 1-Figure 9 A cell culture apparatus comprises a box 1, a plurality of culture dishes, a plurality of pistons 3, an adding mechanism 4, a plurality of first telescopic rods 408 and a plurality of brackets 6.

[0052] like Figure 1 As shown, a plurality of workstations are arranged in the circumferential direction inside the box 1, and a plurality of culture vessels 2 are detachably arranged on the workstations; the culture vessel 2 has a culture chamber 2a, and the culture vessel 2 also has a flow channel 2b, one end of the flow channel 2b passes through the culture vessel 2, and the other end is connected to the culture chamber 2a.

[0053] When culturing cells, first take out the culture vessel 2 from the work station, add the culture medium and the cells to be cultured into the culture chamber 2a, and put the culture vessel 2 back into the work station; add the culture medium into the flow channel 2b, and when the culture medium needs to be supplemented into the culture chamber 2a, the culture medium in the flow channel 2b flows into the culture chamber 2a.

[0054] The flow channel 2b is L-shaped, so that there is no dead corner in the flow channel 2b, and the culture solution is prevented from accumulating at the bottom of the flow channel 2b. All the culture solution in the flow channel 2b can enter the culture chamber 2a along the flow channel 2b.

[0055] It should be noted that Figure 1Only a part of the schematic diagram of the box 1 is shown. The box 1 has a constant temperature function, and since the box 1 is not the improvement of this embodiment, it will not be described in detail here.

[0056] like Figure 2 and Figure 3 As shown, a one-way valve 7 is provided at the flow channel 2b, so that the flow channel 2b is connected to the culture chamber 2a in one direction, that is, the culture fluid in the flow channel 2b can enter the culture chamber 2a through the one-way valve 7, while the culture fluid in the culture chamber 2a cannot enter the flow channel 2b.

[0057] Specifically, the one-way valve 7 includes an elastic member 73, a valve port 72 and a valve core 71. The valve port 72 is arranged in the flow channel 2b. The elastic member 73 is clamped in the flow channel 2b and connected to the valve core 71. The elastic member 73 makes the valve core 71 abut against the valve port 72 to close the valve port 72. When the liquid in the flow channel 2b reaches a maximum value, the pressure applied by the liquid to the valve core 71 is less than the pressure applied by the elastic member 73 to the valve core 71.

[0058] In the absence of external force, the one-way valve 7 is a normally closed valve, which can prevent the culture fluid in the flow channel 2b from spontaneously passing through the one-way valve 7 and entering the culture chamber 2a; only when the culture chamber 2a needs to be supplemented with culture fluid, the culture fluid in the flow channel 2b is acted upon by an external force, pushing the valve core 71 to move and opening the valve port 72, so that the culture fluid in the flow channel 2b passes through the one-way valve 7 and enters the culture chamber 2a.

[0059] In this embodiment, the elastic member 73 is preferably a coil spring.

[0060] like Figure 2 and Figure 4 As shown, a plurality of pistons 3 are respectively arranged in the flow channel 2b and can move along the flow channel 2b; the piston 3 can be opened and closed. When the piston 3 is closed, the piston 3 moves downward to apply pressure to the culture liquid in the flow channel 2b, so that the culture liquid pushes open the valve core 71 and enters the culture chamber 2a through the valve port 72; when the piston 3 is opened, the culture liquid can be supplemented into the flow channel 2b through the piston 3 to prevent the culture liquid in the flow channel 2b from being exhausted.

[0061] Specifically, Figure 5-Figure 7As shown, the piston 3 includes a mounting ring 31, a rotating disk 32 and a plurality of closing plates 33. The mounting ring 31 is slidably arranged in the flow channel 2b, the rotating plate 401 is rotatably arranged in the mounting ring 31, and the plurality of closing plates 33 are slidably arranged on the rotating plate 401. As the rotating plate 401 rotates, it moves inward or outward, so that the piston 3 is opened or closed. A slide groove 2c is provided in the flow channel 2b, and the slide groove 2c has a linear structure and a spiral structure, and the spiral structure is located above the linear structure. The side wall of the mounting ring 31 has a through groove 31a, and the rotating disk 32 has a protrusion 321. The protrusion 321 passes through the through groove 31a and is embedded in the slide groove 2c, and can slide along the slide groove 2c.

[0062] The piston 3 can move up and down along the slide groove 2c. When the piston 3 moves downward, pressure is applied to the culture solution in the flow channel 2b, so that the culture solution can enter the culture chamber 2a through the one-way valve 7; when the piston 3 moves upward, the protrusion 321 moves along the spiral structure, driving the rotating disk 32 to rotate, so that the several closing plates 33 move outward, opening the piston 3, making it convenient to replenish the culture solution into the flow channel 2b and avoiding the culture solution in the flow channel 2b from being exhausted; at the same time, since the piston 3 moves upward, there is a certain distance between the piston 3 and the liquid level of the culture solution in the flow channel 2b, making it convenient to replenish the culture solution into the flow channel 2b and avoiding the culture solution from submerging the piston 3.

[0063] The mounting ring 31 has a first through hole extending therethrough, and the rotating disk 32 has a second through hole extending therethrough, and the first through hole and the second through hole are located on the same axis. As the rotating disk 32 rotates, the plurality of closing plates 33 move inward or outward, thereby opening or closing the second through hole, thereby achieving the purpose of opening or closing the piston 3; the middle portion of the mounting plate 402 also has a cavity structure, and the rotating disk 32 and the plurality of closing plates 33 are all arranged in the cavity structure.

[0064] A plurality of third arc grooves 32 a are provided on the rotating disk 32 , one end of the closing plate 33 is embedded in the third arc groove 32 a and can move along the third arc groove 32 a . As the rotating disk 32 rotates, the closing plate 33 slides along the third arc groove 32 a , thereby opening or closing the piston 3 .

[0065] like Figure 1 As shown, a plurality of second telescopic rods 5 are respectively arranged in the box body 1 through the brackets 6, and the second telescopic rods 5 are arranged directly above the flow channel 2b. When the second telescopic rods 5 are extended, the free ends can abut against the piston 3, driving the piston 3 to move downward, thereby applying pressure to the culture fluid in the flow channel 2b, so that the culture fluid enters the culture chamber 2a through the one-way valve 7.

[0066] like Figure 1 , Figure 8 and Fig. 9As shown, the adding mechanism 4 includes a rotating plate 401, a mounting plate 402, a first connecting rod 403, a second connecting rod 404, a third connecting rod 405, a fourth connecting rod 406, a first telescopic rod 408 and a pipette gun 407;

[0067] The rotating plate 401 is rotatably set at the inner top of the box body 1, and the mounting plate 402 is set at the bottom of the rotating plate 401; one end of the first connecting rod 403 is rotatably connected to the mounting plate 402, the middle part of the third connecting rod 405 is rotatably connected to the mounting plate 402, one end of the second connecting rod 404 is rotatably connected to the other end of the first connecting rod 403, and the other end of the second connecting rod 404 is rotatably connected to one end of the third connecting rod 405; the other end of the third connecting rod 405 is rotatably connected to the middle part of the fourth connecting rod 406, one end of the fourth connecting rod 406 is slidably connected to the rotating plate 401, and the other end is connected to the pipette gun 407; the two ends of the first telescopic rod 408 are rotatably connected to the rotating plate 401 and the first connecting rod 403 respectively.

[0068] By extending and shortening the first telescopic rod 408, the first connecting rod 403 rotates, and driven by the second connecting rod 404, the third connecting rod 405 and the fourth connecting rod 406, the pipette gun 407 moves obliquely downward and approaches the culture vessel 2. When it reaches the bottom dead point, the liquid outlet of the pipette gun 407 is just above the flow channel 2b, so that the culture fluid can be added to the flow channel 2b; alternatively, the pipette gun 407 moves obliquely upward and away from the culture vessel 2.

[0069] When the pipette 407 moves obliquely downward and approaches the culture vessel 2, the second telescopic rod 5 contracts upward to the shortest state, and there is a gap between the free end of the second telescopic rod 5 and the top of the culture vessel 2. When the pipette 407 moves obliquely downward and reaches the lowest position, it just passes through the gap, thereby reducing the height difference between the pipette 407 and the culture vessel 2, and can effectively reduce the splashing of culture solution added into the flow channel 2b by the pipette 407; when the pipette 407 moves obliquely upward and away from the culture vessel 2, the horizontal plane where the lowest point of the pipette 407 is located is higher than the horizontal plane where the highest point of the bracket 6 and the second telescopic rod 5 are located, so that the rotating plate 401 can rotate, thereby solving the problem of motion interference between the pipette 407 and the first telescopic rod 408 and the bracket 6, so as to facilitate the pipette 407 to supplement culture solution into other flow channels 2b.

[0070] In this embodiment, a first arc groove 402a and a second arc groove 402b are provided on the connecting plate; the end of the second connecting rod 404 is embedded in the first arc groove 402a, can move along the first arc groove 402a, and is rotatably connected to the end of the third connecting rod 405; the end of the fourth connecting rod 406 is embedded in the second arc groove 402b, and can move along the second arc groove 402b.

[0071] A first magnet is disposed on the first connecting rod 403, and a second magnet is disposed on the mounting ring 31, and the first magnet and the second magnet attract each other. The first connecting rod 403 has an extended end, and the first magnet is disposed at the extended end. When the liquid transfer gun 407 moves obliquely downward to the dead point position, the extended end of the first connecting rod 403 is just located directly above the piston 3. Under the action of the magnetic force, the piston 3 moves upward, and the protrusion 321 moves along the spiral structure, driving the rotating disk 32 to rotate, so that the plurality of closing plates 33 move outward, opening the piston 3, and the liquid transfer gun 407 can add culture fluid into the flow channel 2b.

[0072] It should be noted that the pipette 407 is connected to a hose, through which the culture solution can be added to the pipette 407 .

[0073] The adding mechanism 4 also includes a driving part, which includes a ring gear 409, a gear 410 and a motor 411; the ring gear 409 is sleeved on the rotating plate 401, and the gear 410 is rotatably set on the inner top of the box body 1 and meshes with the ring gear 409; the motor 411 is set on the top of the box body 1, and the output shaft passes through the box body 1 and is connected to the gear 410 to drive the gear 410 to rotate, so that the ring gear 409 drives the rotating plate 401 to rotate, so that the pipette gun 407 can add culture fluid to the flow channel 2b of different culture vessels 2.

[0074] In this embodiment, the motor 411 is preferably a servo motor 411, which can accurately control the rotation of the gear 410 so that the liquid outlet of the pipette gun 407 can stay just above the flow channel 2b of the culture vessel 2.

[0075] The working principle of this embodiment is:

[0076] The culture medium and cells to be cultured are placed in the culture vessel 2, and the culture medium is added to the flow channel 2b; when new culture medium needs to be added to the culture vessel 2, the second telescopic rod 5 drives the piston 3 to move downward, and the culture medium in the flow channel 2b is acted upon by an external force, pushing the valve core 71 to move, opening the valve port 72, so that the culture medium in the flow channel 2b enters the culture chamber 2a through the one-way valve 7.

[0077] When the culture fluid in the flow channel 2b is consumed to a preset value, the free end of the second telescopic rod 5 is shortened to the shortest, and the first telescopic rod 408 is shortened to drive the first connecting rod 403 to rotate. Driven by the second connecting rod 404, the third connecting rod 405 and the fourth connecting rod 406, the pipette gun 407 moves obliquely downward and approaches the culture vessel 2. When it reaches the bottom dead center position, the liquid outlet of the pipette gun 407 is just above the flow channel 2b, and the first magnet is just above the second magnet. Under the attraction between the first magnet and the second magnet, the mounting ring 31 moves upward, and the protrusion 321 moves along the spiral structure, driving the rotating disk 32 to rotate, so that the several closing plates 33 move outward, opening the piston 3, and the pipette gun 407 replenishes the culture fluid into the flow channel 2b again.

[0078] After replenishment is completed, the first telescopic rod 408 extends to drive the first connecting rod 403 to rotate, and driven by the second connecting rod 404, the third connecting rod 405 and the fourth connecting rod 406, the pipette gun 407 moves upward and away from the culture vessel 2, and the driving gear 410 rotates, so that the ring gear 409 drives the rotating plate 401 to rotate, so that the pipette gun 407 can add culture fluid to the flow channel 2b of different culture vessels 2.

[0079] After replenishment is completed, the second telescopic rod 5 is extended again to press against the piston 3, so that the piston 3 moves downward. During the downward movement of the piston 3, the protrusion 321 moves along the spiral structure, driving the rotating disk 32 to rotate, so that the closing plates 33 move inward and close the piston 3.

[0080] Embodiment 2:

[0081] This embodiment is applied to the cell culture instrument in Embodiment 1, and includes the following steps:

[0082] Step 100, placing culture fluid and cells to be cultured in the culture vessel 2, and adding culture fluid into the flow channel 2b;

[0083] Step 200: When it is necessary to add new culture solution to the culture vessel 2, the piston 3 moves downward to add the culture solution in the flow channel 2b to the culture vessel 2;

[0084] Step 300: When the culture solution in the flow channel 2b is consumed to a preset value, the piston 3 moves upward and opens, and after new culture solution is added into the flow channel 2b, the piston 3 closes.

[0085] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell culture apparatus, characterized in that: include: The box body has a plurality of workstations arranged inside along the circumferential direction; A plurality of culture vessels are detachably arranged on the workstations; the culture vessels have a culture chamber, and the culture vessels also have a flow channel, one end of the flow channel passes through the culture vessel, and the other end is connected to the culture chamber; a one-way valve is provided at the flow channel, so that the flow channel is connected to the culture chamber in one direction; A plurality of pistons are respectively arranged in the flow channel; the pistons can be controlled to open and close, and can also be controlled to slide along the flow channel.

2. A cell culture apparatus according to claim 1, characterized in that: It also includes an adding mechanism, which includes a rotating plate, a mounting plate, a first connecting rod, a second connecting rod, a third connecting rod, a fourth connecting rod, a first telescopic rod and a pipette gun; The rotating plate is rotatably arranged on the inner top of the box body; the mounting plate is arranged on the bottom of the rotating plate; One end of the first connecting rod is rotatably connected to the mounting plate, the middle portion of the third connecting rod is rotatably connected to the mounting plate, one end of the second connecting rod is rotatably connected to the other end of the first connecting rod, and the other end of the second connecting rod is rotatably connected to one end of the third connecting rod; The other end of the third connecting rod is rotatably connected to the middle part of the fourth connecting rod, one end of the fourth connecting rod is slidably connected to the rotating plate, and the other end is connected to the pipette gun; Two ends of the first telescopic rod are rotatably connected to the rotating plate and the first connecting rod respectively.

3. A cell culture apparatus according to claim 2, characterized in that: The connecting plate is provided with a first arc groove and a second arc groove; the end of the second connecting rod is embedded in the first arc groove, can move along the first arc groove, and is rotatably connected with the end of the third connecting rod; The end of the fourth connecting rod is embedded in the second arc groove and can move along the second arc groove.

4. A cell culture apparatus according to claim 2, characterized in that: The adding mechanism further comprises a driving part, and the driving part comprises a gear ring, a gear and a motor; The gear ring is sleeved on the rotating plate, and the gear is rotatably disposed in the box body and meshes with the gear ring; The motor is used to drive the gear to rotate.

5. A cell culture apparatus according to claim 2, characterized in that: The piston includes a mounting ring, a rotating disk and a plurality of closing plates; The mounting ring is slidably disposed in the flow channel, the rotating plate is rotatably disposed in the mounting ring, and a plurality of closing plates are slidably disposed on the rotating plate and move inward or outward as the rotating plate rotates; A slide groove is provided in the flow channel, and the slide groove has a straight structure and a spiral structure, and the spiral structure is located above the straight structure; the side wall of the mounting ring has a through groove, and the rotating disk has a protrusion, and the protrusion passes through the through groove and is embedded in the slide groove and can slide along the slide groove.

6. A cell culture apparatus according to claim 5, characterized in that: It also includes a plurality of second telescopic rods, which are arranged in the box through brackets; When the second telescopic rod is extended, the free end may abut against the piston.

7. A cell culture apparatus according to claim 6, characterized in that: The first connecting rod is provided with a first magnet, the mounting ring is provided with a second magnet, and the first magnet and the second magnet are attracted to each other.

8. A cell culture apparatus according to claim 5, characterized in that: The rotating disk is provided with a plurality of third arc grooves, one end of the closing plate is embedded in the third arc groove and can move along the third arc groove.

9. A cell culture apparatus according to claim 1, characterized in that: The one-way valve comprises an elastic member, a valve port and a valve core; The valve port is arranged in the flow channel, the elastic member is clamped in the flow channel and connected to the valve core, and the elastic member makes the valve core abut against the valve port; Wherein, when the liquid in the flow channel reaches a maximum value, the pressure applied by the culture fluid to the valve core is less than the pressure applied by the elastic member to the valve core.

10. A cell culture method, applied to the cell culture apparatus according to any one of claims 1 to 9, characterized in that: The following steps are involved: placing culture medium and cells to be cultured in a culture vessel, and adding culture medium into the flow channel; When new culture fluid needs to be added to the culture vessel, the piston moves downward to add the culture fluid in the flow channel to the culture vessel; When the culture fluid in the flow channel is consumed to a preset value, the piston moves upward and opens, and after new culture fluid is added to the flow channel, the piston closes.

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