Composition for replacing antibiotics, broiler feed and application of broiler feed in broiler feeding

Abstract

The invention belongs to the field of livestock and poultry breeding, and relates to development of an antibiotic substitute for inhibiting salmonella growth. The invention provides a composition for replacing antibiotics, a broiler feed and application of the broiler feed in broiler feeding. According to the invention, lactobacillus casei and berberine hydrochloride (BBR) are utilized to carry out different proportions, an in-vitro pathogenic bacterium growth inhibition test is utilized, an optimal combination with the best antibacterial effect is selected, and the effectiveness of the product is verified by utilizing broiler feeding, metabolism and challenge tests. According to the aureomycin substitute disclosed by the invention, the diarrhea rate and the death rate of broiler chickens are respectively reduced by 37% and 80%, and the daily gain and the feed efficiency of the broiler chickens are respectively improved by 4% and 3%; the effect is better than that of antibiotics, and a foundation is laid for replacement of the antibiotics and healthy production of livestock and poultry.

Description

Technical Field

[0001] The invention belongs to the field of livestock and poultry breeding, and relates to the development of an antibiotic substitute for inhibiting the growth of Salmonella. Background Art

[0002] Antibiotic resistance, drug tolerance and residues in livestock products pose a serious threat to livestock and poultry health and human health. It is imperative to find feed additives that can replace antibiotics. With the ban on the use of antibiotics as feed additives in the feed industry, the development and application of antibiotic substitutes has become increasingly important.

[0003] In recent years, the research on substitutes for feed antibiotics has become the focus of scientific and technological workers, and it is imperative to develop low-toxic, high-efficiency and harmless antibacterial additives. Antibiotic substitutes generally have the following characteristics: the substance itself or its metabolites do not affect the palatability of feed, have no toxic side effects on animals, kill or inhibit the growth and reproduction of pathogenic microorganisms, are not easy or do not induce the production of drug-resistant bacteria, and are not easy to induce the production of cross-resistant bacteria, and have a good environment. At present, substitutes for feed antibiotics are constantly being innovated. In summary, the current substitutes for feed antibiotics are mainly as follows: acidifiers, enzyme preparations, oligosaccharides, Chinese herbal medicines and plant extracts, microecological preparations, antimicrobial peptides, etc. However, these substitutes have problems such as low efficiency, imperfect production technology, difficult to ensure product quality, unstable feed processing conditions, and destruction of nutrients in feed to varying degrees. Publication No. CN104304675A discloses an antibacterial feed additive and application, which is composed of lactic acid glyceride, mainly used for crucian carp, and the effect on broilers is unknown. In the field of broiler breeding, the antibiotic chlortetracycline is often used to treat broiler diarrhea and reduce mortality; however, there are problems such as drug resistance, drug tolerance, residues and poor effects. In order to find a feed additive to replace antibiotics, our research group conducted in-depth research. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a composition for replacing antibiotics, a broiler feed and application thereof in broiler breeding.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A composition for replacing antibiotics, comprising berberine hydrochloride and Lactobacillus casei.

[0007] The preservation number of the above-mentioned Lactobacillus casei is CGMCC 1.2884.

[0008] Furthermore, the final concentration of the berberine hydrochloride is 0.002% to 0.008%.

[0009] Preferably, the final concentration of the berberine hydrochloride is ≥ 0.004%. 0.004% or more BBR co-cultured with Salmonella for 24 hours can significantly inhibit the growth of Salmonella; antibiotics (CHH) have a significant inhibitory effect on the growth of Salmonella and are superior to berberine hydrochloride.

[0010] The final concentration of the above Lactobacillus casei is ≥1×10 5 .

[0011] Furthermore, the final concentration of the Lactobacillus casei is 1×10 5 ~1×10 7 CFU / mL, Lactobacillus casei (1×10 5 ~1×10 7 CFU / mL) inhibited the growth of Salmonella. 0.002%~0.008% BBR and 1×10 5 ~1×10 7 CFU / mL of Lactobacillus casei can significantly inhibit the growth of Salmonella, and the antibacterial effect becomes better with the extension of culture time, and its antibacterial effect is better than 150mg / kg chlortetracycline.

[0012] Preferably, the final concentration of berberine hydrochloride is 0.002%, and the final concentration of Lactobacillus casei is 1×10 6 CFU / mL. From an economic point of view, 0.002% BBR and 1×10 6 CFU / mL combination of Lactobacillus casei is more suitable, and the two have additive effects in inhibiting the growth of Salmonella, and their antibacterial effect is better than that of chlortetracycline.

[0013] The application of the above composition in replacing antibiotics to inhibit Salmonella.

[0014] A method for reducing the diarrhea rate, mortality rate and / or promoting the growth of broiler chickens, comprising the steps of: adding the above-mentioned composition to broiler feed. The combination of berberine hydrochloride and Lactobacillus casei can inhibit the growth and reproduction of Salmonella. Broiler feeding and virus attack experiments show that adding the product to broiler feed can reduce the diarrhea rate and mortality rate of broiler chickens, increase the daily weight gain and feed efficiency of broiler chickens, improve the body's antioxidant capacity and immunity, and maintain the integrity of intestinal tissue structure. Its effect is better than that of antibiotics. It provides a new feed additive for replacing antibiotics, healthy breeding of livestock and poultry, and safe production of livestock products.

[0015] Therefore, the present application also claims protection for a broiler feed comprising the above composition.

[0016] The present invention has the following beneficial effects:

[0017] 1. The present invention uses Lactobacillus casei and berberine hydrochloride (BBR) in different ratios, selects the best combination with the best antibacterial effect by means of in vitro test for inhibiting the growth of pathogenic bacteria, and verifies the effectiveness of the product by using broiler feeding, metabolism and challenge tests. 0.004% or more of BBR co-cultured with Salmonella for 24 hours can significantly inhibit the growth of Salmonella; Chlortetracycline (CHH) has a significant inhibitory effect on the growth of Salmonella and is superior to berberine hydrochloride; Lactobacillus casei (1×10 5 ~1×10 7 CFU / mL) inhibited the growth of Salmonella; 0.002% BBR had no significant inhibitory effect on the growth of Lactobacillus casei, thus laying the foundation for the combination of berberine hydrochloride and Lactobacillus casei; 0.002%-0.008% BBR and 1×10 5 ~1×10 7 CFU / mL Lactobacillus casei can significantly inhibit the growth of Salmonella, and the antibacterial effect is better with the extension of culture time, and its antibacterial effect is better than 150mg / kg chlortetracycline. From an economic point of view, 0.002% BBR and 1×10 6 CFU / mL combination of Lactobacillus casei is more suitable, and the two have additive effects in inhibiting the growth of Salmonella, and their antibacterial effect is better than that of chlortetracycline.

[0018] 2. The antibiotic substitute prepared by combining berberine hydrochloride and Lactobacillus casei disclosed in the present application reduces the diarrhea rate and mortality rate of broiler chickens by 37% and 80%, respectively, and increases the daily weight gain and feed efficiency of broiler chickens by 4% and 3%, respectively; its efficacy is better than that of antibiotics, laying a foundation for the replacement of antibiotics and the healthy production of livestock and poultry. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 The effect of antibiotic substitutes on the jejunal tissue morphology of broiler chickens infected with Salmonella; Group A: blank control group; Group B: negative control group; Group C: 150 mg / kg chlortetracycline group; Group D: 0.002% BBR+1×10 6 CFU / mL Lactobacillus casei group. DETAILED DESCRIPTION

[0021] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Unless otherwise specified, the experimental methods used in the following experimental examples are all conventional methods; the materials and reagents used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0023] Test materials

[0024] Lactobacillus casein (CGMCC 1.2884) was purchased from the General Microbiology Center of China National Microbiological Culture Collection Administration. Salmonella was a commercial product. Berberine hydrochloride (BBR) and chlortetracycline hydrochloride (CHH) were provided by Henan Delin Biological Products Co., Ltd.

[0025] MRS medium: 1.5 g tryptone, 1 g yeast extract, 2 g glucose, 0.1 mL Tween, 0.2 g potassium dihydrogen phosphate, 0.5 g sodium acetate, 0.005 g manganese sulfate, 0.2 g ammonium citrate, 0.02 g magnesium sulfate, 100 mL distilled water; solid medium plus 2% (w / v) agar powder, 121°C, 0.15 MPa high pressure sterilization for 20 min. Used for static culture of Lactobacillus casei.

[0026] SS medium (g / L): beef extract 5, peptone 5, bile salt 3.5, agar 17, lactose 10, sodium citrate 8.5, sodium thiosulfate 8.5, 10% ferric citrate solution 10mL, 1% neutral red solution 2.5mL, 0.1% brilliant green solution 0.33mL, pH=7; autoclave at 121℃, 0.15MPa for 20min. Used for the culture and identification of Salmonella.

[0027] Example 1: In vitro antibacterial test of berberine hydrochloride and Lactobacillus casei

[0028] 1 Experiment on the inhibition of Salmonella growth by berberine hydrochloride at different concentrations

[0029] Berberine hydrochloride was prepared into MRS culture solution with final concentrations of 0%, 0.002%, 0.004% and 0.008%, inoculated with 1% Salmonella, cultured at 37°C in a shaker at 60r / min, sampled at 8h, 16h and 24h, and counted on plates using SS culture medium. The viable count of microorganisms is expressed as natural logarithm (lg), and the unit is the number of colonies contained in each milliliter of sample (CFU / mL). The results are shown in Table 1:

[0030] Table 1 Inhibitory effect of different concentrations of berberine hydrochloride on the growth of Salmonella (lg, CFU / mL)

[0031]

[0032]

[0033] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), and the same lowercase letters in the same column indicate no significant differences (P>0.05), the same below.

[0034] As shown in Table 1, different concentrations of BBR co-cultured with Salmonella for 8 to 16 hours had no significant inhibitory effect on the growth of Salmonella; 0.004% or more BBR co-cultured with Salmonella for 24 hours could significantly inhibit the growth of Salmonella. Chlortetracycline (CHH) had a significant inhibitory effect on the growth of Salmonella and was superior to berberine hydrochloride.

[0035] 2 Experiment on the inhibition of Salmonella growth by Lactobacillus casei with different viable counts

[0036] The viable counts were 1×10 5 , 1×10 6 and 1×10 7 CFU / mL of Lactobacillus casei was inoculated with 1% Salmonella in MRS culture medium, cultured in a shaker at 37°C at 60 r / min, and samples were taken at 8h, 16h and 24h, respectively, and plate cultured and counted using SS culture medium. The results are shown in Table 2.

[0037] Table 2 Inhibitory effect of different viable counts of Lactobacillus casei on the growth of Salmonella (lg, CFU / mL)

[0038]

[0039] As shown in Table 2, Lactobacillus casei (1×10 5 ~1×10 7 CFU / mL) had the same inhibitory effect on the growth of Salmonella as 150 mg / kg CHH.

[0040] 3 Effect of berberine hydrochloride on the growth of Lactobacillus casei

[0041] The final concentrations of BBR were 0.002%, 0.004% and 0.008%, respectively, and the viable bacterial count was 1×10 5 , 1×10 6 and 1×10 7CFU / mL of Lactobacillus casei was cultured in MRS culture medium at 37°C in an incubator, and samples were taken at 12h, 24h, 36h and 48h, and plate culture was performed using MRS culture medium for counting. The results are shown in Table 3:

[0042] Table 3 Effect of berberine hydrochloride on the growth of different viable counts of Lactobacillus casei (lg, CFU / mL)

[0043]

[0044]

[0045] As shown in Table 3, 0.002% BBR had no significant inhibitory effect on the growth of Lactobacillus casei, thus laying a foundation for the combination of berberine hydrochloride and Lactobacillus casei.

[0046] 4 Experiment on the inhibitory effect of berberine hydrochloride and Lactobacillus casei on the growth of Salmonella

[0047] The final concentration of BBR was 0.002% to 0.008%, and the number of viable bacteria was 1×10 5 , 1×10 6 and 1×10 7 CFU / mL of Lactobacillus casei were mixed, 1% Salmonella was inoculated into MRS culture medium, and cultured at 37°C in a shaker at 60r / min. Samples were taken at 8h, 16h, and 24h, and plate culture was performed on SS solid culture medium for counting. The results are shown in Table 4:

[0048] Table 4 The inhibitory effect of berberine hydrochloride and Lactobacillus casei on the growth of Salmonella (1g, CFU / mL)

[0049]

[0050] As shown in Table 4, 0.002% to 0.008% BBR berberine hydrochloride and 1×10 5 ~1×10 7 CFU / mL Lactobacillus casei can significantly inhibit the growth of Salmonella, and the antibacterial effect increases with the extension of the culture time; its antibacterial effect is better than 150mg / kg chlortetracycline. From an economic point of view, 0.002% BBR and 1×10 6 CFU / mL combination of Lactobacillus casei is more suitable, and the two have additive effects in inhibiting the growth of Salmonella, and their antibacterial effect is better than that of chlortetracycline.

[0051] Application Example 1

[0052] 1. Feeding experiment design

[0053] 180 healthy male AA broilers at 1 day old were selected and divided into 3 groups, with 6 replicates in each group and 10 chickens in each replicate. The whole feeding experiment was divided into two stages: the early stage (1-21d) and the late stage (22-42d). A multi-layer cage feeding mode was adopted, with free access to food and drinking water and 24h light. Immunization program: 1d chicken Newcastle disease live vaccine, 7d chicken Newcastle disease and infectious bronchitis bivalent live vaccine, drinking water immunization. The experimental design and grouping are as follows:

[0054] Group A: Basic diet

[0055] Group B: basic diet + 150mg / kg chlortetracycline

[0056] Group C: basic diet + 0.002% BBR + 1×10 6 CFU / g Lactobacillus casei

[0057] 2 Diet composition and nutritional level

[0058] The experimental basal diet was formulated based on the NRC (1994) broiler feeding standards, see Table 5.

[0059] Table 5 Composition and nutritional level of broiler diets (%)

[0060]

[0061] Note: Premix includes (per kg of complete feed): VA 12 000IU; VD3 3000IU; VE 20IU; VK31.0mg; VB1 2.0mg; riboflavin (VB2) 6mg; niacin (nicotinic acid) 35mg; choline 1.3g; calcium pantothenate 10mg; VB6 3.5mg; VB 12 0.01mg; Biotin 0.15mg; Folic acid 1.25mg; Copper (copper sulfate) 8mg; Iron (ferrous sulfate) 100mg; Manganese (manganese sulfate) 80mg; Zinc (zinc oxide) 60mg; Iodine I (calcium iodate) 0.45mg; Selenium (sodium selenite) 0.35mg.

[0062] 3. Determination of growth performance indicators

[0063] The initial weight of the broilers, the weight of the broilers after fasting for 12 hours at 21 days and 42 days were recorded. The feed intake of each group of replicate chickens was weighed and recorded every day. The health status, mortality and diarrhea of ​​each group of replicate chickens were observed and recorded. The average daily weight gain (ADG), average daily feed intake (ADFI), feed-to-weight ratio (F / G), mortality rate (MR) and diarrhea rate (DR) of each group of replicate chickens were calculated. The results are shown in Table 6:

[0064] Table 6 Effects of antibiotic alternatives on broiler growth performance (n=6)

[0065]

[0066]

[0067] Note: Group A: blank control group; Group B: 150 mg / kg chlortetracycline group; Group C: 0.002% BBR+1×10 6 CFU / g Lactobacillus casei group

[0068] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), and the same letters indicate no significant differences (P>0.05), the same below.

[0069] As shown in Table 6, compared with the control group, the antibiotic substitute prepared by the combination of berberine hydrochloride and Lactobacillus casei reduced the diarrhea rate and mortality rate of broiler chickens by 37% and 80% respectively, and increased the daily weight gain and feed efficiency of broiler chickens by 4% and 3% respectively; its efficacy is better than that of antibiotics, laying a foundation for the replacement of antibiotics and healthy production of livestock and poultry.

[0070] Example 2: Protective effect of antibiotic alternatives on broiler chickens infected with Salmonella

[0071] 1 Experimental design and grouping

[0072] After the broiler feeding experiment, 60 healthy male AA broilers aged 50 days were selected and divided into 4 groups, with 5 replicates in each group and 3 chickens in each replicate. After each group was fed with the corresponding diet for 1 week, each broiler in the blank control group was gavaged with 2 mL of normal saline, and the broilers in the other groups were gavaged with 2 mL of 2.3×10 9 CFU / mL Salmonella, and then continue to feed for another week, the experimental groups are as follows:

[0073] Group A: basic diet (blank control group)

[0074] Group B: basic diet (negative control group)

[0075] Group C: basic diet + 150mg / kg chlortetracycline

[0076] Group D: basic diet + 0.002% BBR + 1×10 6 CFU / mL Lactobacillus casei

[0077] 2. Measurement indicators and methods

[0078] 2.1 Growth performance indicators

[0079] During the entire feeding period, the initial body weight, body weight before the challenge, body weight when diarrhea stopped, and body weight after the end of the experiment were weighed for the broiler chickens. The feed intake of each group of repeated chickens was weighed and recorded every day. The health status, mortality, and diarrhea of ​​each group of repeated chickens were observed and recorded. The average daily weight gain (ADG), average daily feed intake (ADFI), feed-to-weight ratio (F / G), mortality (MR), and diarrhea rate (DR) of each group of repeated chickens were calculated. The diarrhea rate (DR) = total diarrhea days / (total number of chickens × number of experimental days). The results are shown in Table 7:

[0080] Table 7 Effects of antibiotic alternatives on growth performance of broiler chickens challenged with Salmonella

[0081]

[0082] As shown in Table 7, the negative control group (B) had the lowest daily weight gain and the highest diarrhea rate. Compared with the negative control group, the daily weight gain of the antibiotic replacement group (D) increased significantly by 15.02%, the feed-to-meat ratio decreased significantly by 9.15%, and the diarrhea rate decreased significantly by 48.62%. Moreover, all indicators of the antibiotic replacement group were better than those of the antibiotic group (C).

[0083] 2.2 Determination of nutrient metabolic rate

[0084] The full feces collection method was used to collect excrement on the 4th to 6th day after the infection, and the daily feed intake and feces weight were recorded. The collected excrement was thoroughly mixed after removing the debris, and 10% sulfuric acid was added to fix nitrogen, and stored at -20°C. The feed sample and feces sample were dried in a 65°C oven to constant weight and rehydrated for 24 hours before being crushed through a 40-mesh sieve for later use. Samples were taken to determine crude protein, crude fat, calcium and phosphorus. The results are shown in Table 8:

[0085] Table 8 Effects of antibiotic alternatives on nutrient metabolism rates in broilers infected with Salmonella (%)

[0086]

[0087] As shown in Table 8, the metabolic rates of crude protein, crude fat and phosphorus in the negative control group (B) were the lowest; compared with the negative control group, the metabolic rates of crude protein, crude fat and phosphorus in the antibiotic replacement group (D) were significantly increased and higher than those in the antibiotic group (C).

[0088] 2.3 Determination of serum biochemical indicators

[0089] Five chickens with a body weight close to the average were selected from each group for slaughter. Blood was collected from the wing vein of each chicken into a coagulant tube and centrifuged at 3000 rpm for 10 min. The serum was collected and stored at -20°C.

[0090] Determination of serum biochemical indicators: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glucose (GLU), triglyceride (TG), total protein, albumin, globulin, blood urea nitrogen (BUN), and C-reactive protein (CRP) in serum were determined using a biochemical automatic analyzer. The results are shown in Table 9:

[0091] Table 9 Effects of antibiotic substitutes on serum biochemical parameters of broiler chickens infected with Salmonella

[0092]

[0093] Note: Group A: blank control group; Group B: negative control group; Group C: 150 mg / kg chlortetracycline group; Group D: 0.002% BBR+1×10 6 CFU / mL Lactobacillus casei group.

[0094] Note: Data in the same row with different lowercase letters indicate significant differences (P<0.05), and data with the same lowercase letters indicate no significant differences (P>0.05).

[0095] As shown in Table 9, the activities of aspartate transferase, alkaline phosphatase and lactate dehydrogenase in the serum of broilers in the negative control group (B) were significantly higher than those in the other groups, indicating that Salmonella caused damage to organs such as the liver of broilers, and antibiotic substitutes, like antibiotics, have the same effect of protecting tissues and organs from the harm of pathogenic microorganisms. The use of antibiotics significantly increased the albumin content in the serum of broilers, reduced globulin and the albumin / globulin ratio, indicating that the use of antibiotics disrupted the immune system of broilers. The urea nitrogen content in the serum of broilers in the negative control group was significantly higher than that in the other groups, indicating that protein metabolism was affected by Salmonella, which is consistent with the low protein metabolic rate. The C-reactive protein content in the serum of broilers in the negative control group was significantly higher than that in other groups, indicating that Salmonella caused an inflammatory response in the body, while antibiotics and antibiotic substitutes have the effect of reducing the inflammatory response caused by Salmonella.

[0096] Determination of antioxidant and immune indexes: The contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), total antioxidant capacity (T-AOC), malondialdehyde (MDA) and immune indexes (IgA, IgG, IgM) in broiler serum were determined using ELISA kits from Shanghai ELISA Biotechnology Co., Ltd. (Shanghai, China). The results are shown in Tables 10 and 11:

[0097] Table 10 Effects of antibiotic substitutes on serum antioxidant indexes of broiler chickens infected with Salmonella

[0098]

[0099] Note: Group A: blank control group; Group B: negative control group; Group C: 150 mg / kg chlortetracycline group; Group D: 0.002% BBR+1×10 6 CFU / mL Lactobacillus casei group.

[0100] Note: Data in the same column with different lowercase letters indicate significant differences (P<0.05), and data with the same letters indicate no significant differences (P>0.05), the same below.

[0101] Table 11 Effects of antibiotic substitutes on serum immune parameters of broiler chickens infected with Salmonella (μg / mL)

[0102]

[0103] Note: Group A: blank control group; Group B: negative control group; Group C: 150 mg / kg chlortetracycline group; Group D: 0.002% BBR+1×10 6 CFU / mL Lactobacillus casei group.

[0104] The results in Tables 10 and 11 show that Salmonella infection in broilers can reduce their serum antioxidant indices and the levels of IgG and IgM, while the addition of antibiotics and antibiotic substitutes can improve serum antioxidant indices and immune parameters.

[0105] 2.4 Determination of jejunal tissue morphology

[0106] The middle section of the jejunum, about 2 cm in length, was taken and rinsed with saline and fixed in 10% formalin for tissue sectioning. After paraffin embedding, section dewaxing, hematoxylin staining, washing, dehydration, and sealing, the jejunum morphology was analyzed under an optical microscope, and the villus length and crypt depth were calculated. Figure 1 As shown by Figure 1 It can be seen that the jejunal tissue mucosa of broiler chickens infected with Salmonella was damaged (Group B), and antibiotics (Group C) and antibiotic substitutes (Group D) had the function of slowing down the damage of Salmonella to the jejunal tissue mucosa of broiler chickens.

[0107] From the above, we can see that the antibiotic substitute prepared by the combination of berberine hydrochloride and Lactobacillus casei has a good function of inhibiting the growth of Salmonella and alleviating its intestinal damage to broiler chickens, can reduce the diarrhea rate and mortality rate of broiler chickens, increase daily weight gain and feed efficiency, and improve the body's antioxidant properties and immunity. Its efficacy is better than antibiotics, laying the foundation for the replacement of antibiotics and the healthy production of livestock and poultry.

[0108] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition for replacing antibiotics, characterized in that: The composition comprises berberine hydrochloride and lactobacillus casei.

2. The composition for replacing antibiotics according to claim 1, characterized in that: The preservation number of the Lactobacillus casei is CGMCC 1.2884.

3. The composition for replacing antibiotics according to claim 2, characterized in that: The final concentration of the berberine hydrochloride is 0.002% to 0.008%.

4. The composition for replacing antibiotics according to claim 2, characterized in that: The final concentration of the berberine hydrochloride is ≥0.004%.

5. The composition for replacing antibiotics according to any one of claims 1 to 4, characterized in that: The final concentration of Lactobacillus casei is ≥1×10 5 .

6. The composition for replacing antibiotics according to claim 5, characterized in that: The final concentration of Lactobacillus casei is 1×10 5 ~1×10 7 CFU / mL.

7. The composition for replacing antibiotics according to claim 1 or 2, characterized in that: The final concentration of berberine hydrochloride was 0.002%, and the final concentration of Lactobacillus casei was 1×10 6 CFU / mL.

8. Use of the composition according to any one of claims 1 to 4 and 6 in replacing antibiotics to inhibit Salmonella.

9. A method for reducing the diarrhea rate and mortality rate of broiler chickens and / or promoting the growth of broiler chickens, characterized in that: This is achieved by adding the composition according to any one of claims 1 to 4 and 6 to broiler feed.

10. A broiler feed comprising the composition according to any one of claims 1 to 4 and 6.

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