A tumor detection system based on minimally invasive probes and Raman imaging technology

Abstract

The present invention discloses a tumor detection system based on minimally invasive probe and Raman imaging technology, belonging to the field of tumor detection technology. The present invention mainly includes a transmission device, a minimally invasive probe device, a Raman imaging device and an imaging device. Based on the probe detection technology and the Raman imaging technology, a tumor detection system based on minimally invasive probe and Raman imaging technology is designed, which provides a practical solution for tumor detection, integrates sampling and detection, realizes a certain degree of automation in the process of tumor detection, reduces the difficulty of operation and detection, and realizes automated tumor detection integrating sampling, analysis and detection.

Description

Technical Field

[0001] The present invention belongs to the technical field of tumor detection, and in particular, relates to a tumor detection system based on minimally invasive probes and Raman imaging technology. Background Art

[0002] Currently, the detection methods for urinary system tumors mainly include: liquid biopsy technology, imaging examination, endoscopic examination, tumor marker detection, etc., but these methods have problems such as cumbersome operation, difficulty in real-time monitoring, and low sensitivity of imaging detection. Therefore, a urinary system tumor detection method with simple operation, real-time monitoring and high sensitivity is urgently needed.

[0003] Raman spectroscopy is a molecular structure analysis technology based on the Raman scattering effect, which can provide molecular composition and structural information of substances. Raman spectroscopy can detect changes in the structure of basic components (such as proteins, nucleic acids, lipids and carbohydrates) in tumor cells, and has the potential to identify tumors at the molecular level.

[0004] Clinically, Raman spectroscopy has been used to distinguish bladder cancer from precancerous lesions, and has shown high accuracy in distinguishing normal kidney tissue from renal cell carcinoma tissue. It has also been used to detect prostate cancer and can distinguish different prostate cancer cell lines. Raman spectroscopy has significant application prospects in tumor detection, especially in providing rapid, non-invasive and highly sensitive detection methods.

[0005] However, in current practical applications, there is no relevant equipment for using Raman imaging technology in the field of cancer cell detection. Most of the sampling and detection work are carried out separately, which is cumbersome to use and requires staff to be able to operate sampling, detection and other equipment more skillfully.

[0006] Therefore, a new integrated design is needed that is simpler to operate and can complete sampling and screening testing at one time. Summary of the invention

[0007] According to the probe detection technology and Raman imaging technology, the present invention designs a tumor detection system based on minimally invasive probes and Raman imaging technology, providing an integrated sampling and detection solution for tumor detection.

[0008] In view of the above problems existing in the prior art, the object of the present invention is to provide a tumor detection system based on minimally invasive probes and Raman imaging technology.

[0009] In order to solve the above problems, the technical solution adopted by the present invention is as follows:

[0010] A tumor detection system based on minimally invasive probe and Raman imaging technology, comprising a transmission device, a minimally invasive probe device, a Raman imaging device and an imaging device;

[0011] The transmission device, minimally invasive probe device, Raman imaging device and imaging device are connected in sequence from left to right;

[0012] The transmission device comprises a driving motor, a transmission gear set and a connecting rod, wherein the driving motor is connected to the connecting rod through the transmission gear set, one end of the connecting rod is connected to the transmission gear set, and the other end is connected to the minimally invasive probe device;

[0013] The minimally invasive probe device comprises a probe, a mounting frame, a probe base, a telescopic rod, a movable plate and a probe motor. The probe base is rotatably arranged above the movable plate. The mounting frame is movably connected to the upper surface of the probe base. The probe is arranged at the top of the mounting frame. The telescopic rod is an electric telescopic rod. One end of the telescopic rod is fixedly connected to the probe, and the other end is connected to the probe base ball. The probe motor is arranged on the lower surface of the probe base. A groove is provided on the movable plate. The probe motor is arranged in the groove provided on the movable plate. The probe base is fixedly connected to the output shaft of the probe motor. Baffles are arranged on both sides of the movable plate.

[0014] The Raman imaging device comprises a loading part and a light source part;

[0015] The object-carrying part and the light source part are connected via an optical path system;

[0016] The object-carrying part includes a glass slide, an object stage, an object stage light source, an objective lens and a camera. The glass slide is mounted on the object stage. A object stage light source is disposed below the object stage. An objective lens is disposed above the object stage. An optical path system is disposed above the objective lens. A camera is disposed above the optical path system.

[0017] The light source part includes a grating light source and a laser light source. The light paths of the grating light source and the laser light source are superimposed on each other through a refractor and a reflector and then output to the optical path system. The light output by the light source part is irradiated toward the stage through the optical path system.

[0018] The driving motor, the telescopic rod, the probe motor and the camera are electrically connected to the imaging device respectively.

[0019] Furthermore, the transmission gear group includes: a motor gear, a first-stage reduction gear, a first transmission shaft, a second-stage reduction gear, a third-stage reduction gear and a second transmission shaft. The motor gear is fixedly connected to the output shaft of the driving motor, the first transmission shaft is rotatably arranged beside the first-stage reduction gear, the first-stage reduction gear and the second-stage reduction gear are fixedly installed on the first transmission shaft and can rotate with the first transmission shaft, the first-stage reduction gear is meshed with the motor gear, the second transmission shaft is rotatably arranged beside the first transmission shaft, the third-stage reduction gear is fixedly installed on the second transmission shaft and can rotate with the second transmission shaft, the third-stage reduction gear is meshed with the second-stage reduction gear, and the third-stage reduction gear is located on the side close to the minimally invasive probe device.

[0020] Furthermore, the mounting frame includes a first connecting rod, a second connecting rod and a connecting rod base, the connecting rod base is installed and fixed on the probe base, one end of the first connecting rod is rotatably connected to the connecting rod base, the other end of the first connecting rod is rotatably connected to the second connecting rod, and the other end of the second connecting rod is rotatably connected to the probe.

[0021] Furthermore, one end of the connecting rod close to the three-stage reduction gear is fixedly connected to the three-stage reduction gear, and one end of the connecting rod close to the movable plate is provided with a through groove along the length direction of the connecting rod, and a sliding rod is fixedly connected to the movable plate, and the sliding rod can move in the through groove.

[0022] Furthermore, the optical path system includes an upper optical path and a right optical path, the right optical path includes a first reflector, a second reflector, a lens and a reflector, and the upper optical path includes a third reflector and a fourth reflector.

[0023] Furthermore, the light source part includes a grating light source and a laser light source, and the light paths of the grating light source and the laser light source are output to the upper light path through the right light path, and the light output from the upper light path is finally irradiated to the slide.

[0024] Furthermore, the Raman imaging device also includes a grating.

[0025] Furthermore, a refrigeration unit is provided outside the Raman imaging device.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] Compared with the existing technology, this solution provides a tumor detection system that is simpler to operate. Based on a minimally invasive probe structure and Raman imaging technology, it does not require staff to be proficient in sampling and detection devices to collect samples and detect tumors. In addition, this solution also integrates sampling and detection, further reducing the difficulty of operation and realizing automated tumor detection that integrates sampling, analysis, and detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 A front view of a tumor detection system based on minimally invasive probes and Raman imaging technology;

[0029] Figure 2 A cross-sectional view of a tumor detection system based on minimally invasive probes and Raman imaging technology;

[0030] Figure 3 An axonometric diagram of a tumor detection system based on minimally invasive probes and Raman imaging technology;

[0031] Figure 4 It is a schematic diagram of the structure of each component of the transmission device;

[0032] Figure 5 is a schematic diagram of the structure of a minimally invasive probe device;

[0033] Figure 6 This is a disassembled diagram of the minimally invasive probe device;

[0034] Figure 7 is a side view of a partial structure of a minimally invasive probe device;

[0035] Figure 8 It is a schematic diagram of the structure of a minimally invasive probe device and a Raman imaging device;

[0036] Fig. 9 is a cross-sectional view of a Raman imaging device C;

[0037] In the figure: A, transmission device, B, minimally invasive probe device, C, Raman imaging device, D, imaging device, 1, motor, 2, motor gear, 3, primary reduction gear, 4, first transmission shaft, 5, secondary reduction gear, 6, tertiary reduction gear, 7, second transmission shaft, 8, connecting rod, 9, sliding rod, 10, moving plate, 11, probe base, 12, first connecting rod, 13, second connecting rod, 14, probe, 15, pin, 16, connecting rod base, 17, telescopic rod, 18, probe Needle motor, 19, slide, 20, stage, 21, stage up and down adjustment knob, 22, stage light source, 23, carrier, 24, stage left and right adjustment knob, 25, objective lens, 26, objective lens stage, 28, camera, 29, refrigeration unit, 30, grating, 32, laser, 33, laser housing, 35, first reflector, 36, second reflector, 37, lens, 38, reflector, 39, grating light source, 40, third reflector, 41, fourth reflector. DETAILED DESCRIPTION

[0038] The present invention is further described below in conjunction with specific embodiments.

[0039] like Figure 1 , Figure 2 and Figure 3 As shown, this embodiment discloses a tumor detection system based on minimally invasive probe and Raman imaging technology, the detection system consists of a transmission device A, a minimally invasive probe device B, a Raman imaging device C, and an imaging device D, wherein the transmission device, minimally invasive probe device, Raman imaging device and imaging device are arranged from left to right in sequence.

[0040] Under the operation of the transmission system A, the minimally invasive probe device B can be pushed outward to facilitate sampling by the minimally invasive probe device B; the probe in the minimally invasive probe device B can be extended and rotated, and after sampling, it is sent to the Raman imaging device C for subsequent observation and analysis; the imaging device D collects image information and Raman spectrum data of the sample, displays the collected image information, and processes and analyzes the collected Raman spectrum data.

[0041] Transmission device A Figure 4 As shown, the transmission device A includes a driving motor 1, a transmission gear set and a connecting rod 8. The driving motor 1 is connected to the connecting rod 8 through the transmission gear set. One end of the connecting rod 8 is connected to the transmission gear set, and the other end is connected to the minimally invasive probe device B.

[0042] The transmission gear set includes: a motor gear 2, a primary reduction gear 3, a first transmission shaft 4, a secondary reduction gear 5, a tertiary reduction gear 6 and a second transmission shaft 7. The motor gear 2 is fixedly connected to the output shaft of the driving motor 1. The first transmission shaft 4 is rotatably arranged beside the primary reduction gear 3. The primary reduction gear 3 and the secondary reduction gear 5 are fixedly mounted on the first transmission shaft 4 and can rotate with the first transmission shaft 4. The primary reduction gear 3 is meshed with the motor gear 2. The second transmission shaft 7 is rotatably arranged beside the first transmission shaft 4. The tertiary reduction gear 6 is fixedly mounted on the second transmission shaft 7 and can rotate with the second transmission shaft 7. The tertiary reduction gear 6 is meshed with the secondary reduction gear 5. The tertiary reduction gear 6 is located on a side close to the minimally invasive probe device B. One end of the connecting rod 8 is fixedly connected to the tertiary reduction gear 6, and the other end enters into the space of the minimally invasive probe device B.

[0043] like Figure 5 , Figure 6 as well as Figure 7As shown, the minimally invasive probe device B includes a probe 14, a mounting frame, a probe base 11, a telescopic rod 17, a movable plate 10 and a probe motor 18. The probe base 11 is rotatably arranged above the movable plate 10. The mounting frame is movably connected to the upper surface of the probe base 11. The probe 14 is arranged at the top of the mounting frame. One end of the telescopic rod 17 is fixedly connected to the probe 14, and the other end is ball-connected to the probe base 11. The ball connection method can limit its maximum inclination angle under the premise that the telescopic rod 17 can rotate freely, so as to prevent the entire mounting frame structure from falling on the probe base 11 due to lack of support, and the telescopic rod 17 is an electric telescopic rod, and its extension and contraction can be controlled by an electrical signal; the probe motor 18 is arranged on the lower surface of the probe base 11, a groove is provided on the movable plate 10, and the probe motor 18 is arranged in the groove provided on the movable plate 10. The probe base 11 is fixedly connected to the output shaft of the probe motor 18, and the probe base 11 can rotate relative to the movable plate 10 through the drive of the probe motor 18.

[0044] Baffles are provided on both sides of the moving plate 10 to limit the moving direction of the moving plate 10 so that the moving plate 10 can only move along the direction of the baffles. Figure 5 , the minimally invasive probe device B can be pushed out along the moving direction of the baffle.

[0045] like Figure 5 , Figure 6 , Figure 7 As shown, the mounting frame is a movable multi-section structure, and the sections of the mounting frame are movably connected by a pin structure. Specifically, the mounting frame includes a first connecting rod 12, a second connecting rod 13 and a connecting rod base 16. The connecting rod base 16 is installed and fixed on the probe base 11. One end of the first connecting rod 12 is rotatably connected to the connecting rod base 16, the other end of the first connecting rod 12 is rotatably connected to the second connecting rod 13, and the other end of the second connecting rod 13 is rotatably connected to the probe 14. Specifically, the connecting rod base 16, the first connecting rod 12, the second connecting rod 13 and the probe 14 are rotatably connected by a pin 15 and a pin hole.

[0046] The connecting rod 8 passes through the partition between the transmission device A and the minimally invasive probe device B and is connected to the movable plate 10. Specifically, a through groove along the length direction of the connecting rod 8 is provided at one end of the connecting rod 8 close to the movable plate 10, and a sliding rod 9 is fixedly connected to the movable plate 10, and the sliding rod 9 can move in the through groove.

[0047] When the three-stage reduction gear 6 rotates, the connecting rod 8 rotates along with the rotation of the three-stage reduction gear 6, thereby driving the movable plate 10 to move along with the movement of the connecting rod 8. When the connecting rod 8 moves, the through groove and the slide bar 9 provided therein can slide relative to each other, allowing it to rotate normally. The slide bar 9 will not conflict with the connecting rod 8 when it rotates, thereby causing it to be unable to drive the movable plate 10 to move. Finally, the movable plate 10 is moved along the direction of the baffles on both sides by rotating the connecting rod 8.

[0048] The movable plate 10 is pushed out from the space of the minimally invasive probe device B by the movement of the connecting rod 8. When the connecting rod 8 rotates in the opposite direction, the movable plate 10 is retracted into the space of the minimally invasive probe device B. After the movable plate 10 is pushed out, the probe 14 can be controlled to take samples. After the movable plate 10 is retracted, the probe 14 can be controlled to move to the top of the stage 20 of the Raman imaging device C to load the sample onto the slide 19.

[0049] In addition, the drive motor 1, the probe motor 18 and the telescopic rod 17 are all connected to the display device D signal, wherein the drive motor 1, the probe motor 18 and the telescopic rod 17 are all electric structures in the present embodiment, especially the telescopic rod 17, which can control the extension and retraction length of the telescopic rod 17 through signals, so as to better control the vertical and horizontal positions of the probe 14.

[0050] like Figure 8 and Fig. 9 As shown, the Raman imaging device C includes a loading part and a light source part, and the loading part and the light source part are connected through an optical path system, and the optical path system includes an upper optical path and a right optical path.

[0051] The loading part includes a glass slide 19, a stage 20, a stage up and down adjustment knob 21, a stage light source 22, a carrier frame 23, a stage left and right adjustment knob 24, an objective lens 25, an objective lens stage 26 and a camera 28. The glass slide 19 is mounted on the stage 20, the stage 20 is arranged above the carrier frame 23, the stage light source 22 is arranged below the carrier frame 23, the objective lens 25 and the objective lens stage 26 are located above the stage 20, the objective lens 25 is installed and fixed below the objective lens stage 26, the upper optical path is above the objective lens stage 26, and a camera 28 is arranged above the upper optical path.

[0052] The stage 20 is mainly used to place the glass slide 19, and the camera 28 is used to obtain the image of the sample on the glass slide 19. The height position and the left and right position of the glass slide 19 can be adjusted by the stage up and down adjustment knob 21 and the stage left and right adjustment knob 24. By adjusting the stage up and down adjustment knob 21 and the stage left and right adjustment knob 24, the camera 28 can obtain a better imaging effect.

[0053] The light source part includes a grating light source 39 and a laser light source. The light paths of the grating light source 39 and the laser light source are superimposed on each other through a refractor and a reflector and then output to the optical path system. The light finally output by the light source part is irradiated to the stage 20 through the upper optical path. Among them, the laser light source includes a laser 32 and a laser housing 33. The function of the laser light source is to irradiate the sample and excite the sample to generate Raman scattered light. These scattered lights contain information such as molecular vibration and rotation of the sample, which is the basis of analysis.

[0054] In Raman spectroscopy, in addition to the laser light source used to excite the sample to generate Raman scattering, another light source is sometimes used. In this embodiment, the grating light source 39 is used to have the following effects:

[0055] 1. Improve the signal-to-noise ratio: In some cases, the sample may produce a strong fluorescence background, which will drown out the Raman signal and make it difficult to obtain a clear Raman spectrum. Using a grating light source39, especially light with a slightly different wavelength from the original driving light, can help suppress or filter out the effects of fluorescence radiation. By subtracting the two spectral results obtained after irradiating the sample with two beams of light under as similar conditions as possible, most of the wavelength-insensitive fluorescence signals can be eliminated;

[0056] 2. Enhance the Raman signal: In some cases, the Raman scattered signal may be very weak. Using a grating light source 39 can increase the light intensity, thereby enhancing the Raman signal and making the signal easier to be detected by the detector;

[0057] 3. Realize resonant Raman scattering: In certain applications, such as resonant Raman scattering, the wavelength of the grating light source 39 may be close to or coincide with the electronic absorption peak of the sample, which can significantly enhance the intensity of the specific Raman peak and make the detection more sensitive.

[0058] The right optical path includes a first reflector 35, a second reflector 36, a lens 37 and a reflector 38, and the upper optical path includes a third reflector 40 and a fourth reflector 41. The excitation light provided by the laser light source is reflected by the first reflector 35 and the second reflector 36 in turn to direct the laser light to the lens 37, and finally the light of the laser light source is reflected by the lens 37 to the third reflector 40, and then the light is finally output to the glass slide 19 through the reflection of the fourth reflector 41. The light emitted by the grating light source 39 is reflected by the reflector 38 to the lens 37, and then refracted by the lens 37 to the third reflector 40, and then reflected by the third reflector 40 to the fourth reflector 41, and then the light is finally output to the glass slide 19 through the reflection of the fourth reflector 41. At this point, the light overlap of the grating light source 39 and the laser light source is completed to meet the light requirements of Raman imaging. The Raman imaging device C further includes a grating 30, which is used to decompose the collected Raman scattered light by wavelength so as to detect it on different pixels of the detector (the camera 28 in this text). In this way, the light received on each pixel corresponds to a specific wavelength, so that the spectral information of the sample can be obtained.

[0059] The Raman imaging device C further includes a refrigeration unit 29 disposed outside the light source part, and the refrigeration unit 29 is used to dissipate heat from the light source part.

[0060] The imaging device D and the Raman imaging device C are electrically connected. The imaging device D is a relatively common microcomputer and display. Through the imaging device D, various values ​​of the light source can be adjusted, and the image of the slide 19 taken by the camera 28 can also be obtained. The computer and the display of the imaging device D are common equipment. The analysis program that can be used is the spectrum analysis software OPUS developed by Bruker, which supports the collection, processing and analysis of Raman spectrum data. In this embodiment, the wavelength of the laser emitted by the laser 32 is the common wavelength of the Raman spectrum analysis laser, which is 532 nm, 633 nm, and 785 nm respectively. After testing, the imaging device D can normally collect, process and analyze Raman spectrum data under the irradiation of the laser light source of the above specifications.

[0061] The actual operation of this detection system is as follows:

[0062] Sampling process: When it is necessary to sample, the driving motor 1 is started by controlling the imaging device D. After receiving the signal, the driving motor 1 rotates clockwise, driving the motor gear 2 to rotate clockwise, and the first-stage reduction gear 3 rotates counterclockwise; the second-stage reduction gear 5 rotates counterclockwise, driving the third-stage reduction gear 6 meshing with it to rotate clockwise, and then driving the moving plate 10 to move outward through the connecting rod 8. After the moving plate 10 is moved outward, the imaging device D sends a signal to the probe motor 18, and the probe motor 18 rotates after receiving the electrical signal until the probe 14 points to the sample direction, and then sends an electrical signal to the telescopic rod 17 through the imaging device D to control the telescopic rod 17 to extend and retract until the probe 14 is aligned with the sample position, and then sampling is performed. After sampling is completed, the moving plate 10 and the probe 14 return to the minimally invasive probe device B, and then the sample on the probe 14 is placed on the slide 19 by controlling the probe motor 18 and the telescopic rod 17, and then the probe 14 is reset.

[0063] Detection process: After the sample is moved onto the glass slide 19, the Raman imaging device C starts to work, and the camera 28 transmits the acquired image information to the Raman spectrum analysis software in the imaging device D to determine whether the sample information is a cancer cell.

Claims

1. A tumor detection system based on minimally invasive probes and Raman imaging technology, characterized in that: It includes a transmission device, a minimally invasive probe device, a Raman imaging device and a display device; The transmission device, minimally invasive probe device, Raman imaging device and imaging device are connected in sequence from left to right; The transmission device comprises a driving motor, a transmission gear set and a connecting rod, wherein the driving motor is connected to the connecting rod through the transmission gear set, one end of the connecting rod is connected to the transmission gear set, and the other end is connected to the minimally invasive probe device; The minimally invasive probe device comprises a probe, a mounting frame, a probe base, a telescopic rod, a movable plate and a probe motor. The probe base is rotatably arranged above the movable plate. The mounting frame is movably connected to the upper surface of the probe base. The probe is arranged at the top of the mounting frame. The telescopic rod is an electric telescopic rod. One end of the telescopic rod is fixedly connected to the probe, and the other end is connected to the probe base ball. The probe motor is arranged on the lower surface of the probe base. A groove is provided on the movable plate. The probe motor is arranged in the groove provided on the movable plate. The probe base is fixedly connected to the output shaft of the probe motor. Baffles are arranged on both sides of the movable plate. The Raman imaging device comprises a loading part and a light source part; The object-carrying part and the light source part are connected via an optical path system; The object-carrying part includes a glass slide, an object stage, an object stage light source, an objective lens and a camera. The glass slide is mounted on the object stage. A object stage light source is disposed below the object stage. An objective lens is disposed above the object stage. An optical path system is disposed above the objective lens. A camera is disposed above the optical path system. The light source part includes a grating light source and a laser light source. The light paths of the grating light source and the laser light source are superimposed on each other through a refractor and a reflector and then output to the optical path system. The light output by the light source part is irradiated toward the stage through the optical path system. The driving motor, the telescopic rod, the probe motor and the camera are electrically connected to the imaging device respectively.

2. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 1, characterized in that: The transmission gear group includes: a motor gear, a first-stage reduction gear, a first transmission shaft, a second-stage reduction gear, a third-stage reduction gear and a second transmission shaft. The motor gear is fixedly connected to the output shaft of the driving motor. The first transmission shaft is rotatably arranged beside the first-stage reduction gear. The first-stage reduction gear and the second-stage reduction gear are fixedly installed on the first transmission shaft and can rotate with the first transmission shaft. The first-stage reduction gear is meshed with the motor gear. The second transmission shaft is rotatably arranged beside the first transmission shaft. The third-stage reduction gear is fixedly installed on the second transmission shaft and can rotate with the second transmission shaft. The third-stage reduction gear is meshed with the second-stage reduction gear. The third-stage reduction gear is located on a side close to the minimally invasive probe device.

3. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 1, characterized in that: The mounting frame includes a first connecting rod, a second connecting rod and a connecting rod base. The connecting rod base is installed and fixed on the probe base. One end of the first connecting rod is rotatably connected to the connecting rod base, the other end of the first connecting rod is rotatably connected to the second connecting rod, and the other end of the second connecting rod is rotatably connected to the probe.

4. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 2, characterized in that: The end of the connecting rod close to the three-stage reduction gear is fixedly connected to the three-stage reduction gear, and the end of the connecting rod close to the movable plate is provided with a through groove along the length direction of the connecting rod. The movable plate is fixedly connected with a sliding rod, and the sliding rod can move in the through groove.

5. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 1, characterized in that: The optical path system comprises an upper optical path and a right optical path, the right optical path comprises a first reflector, a second reflector, a lens and a reflector, and the upper optical path comprises a third reflector and a fourth reflector.

6. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 5, characterized in that: The light source part includes a grating light source and a laser light source. The light paths of the grating light source and the laser light source are output to the upper light path through the right light path. The light output from the upper light path is finally irradiated to the slide.

7. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 1, characterized in that: The Raman imaging device further comprises a grating.

8. The tumor detection system based on minimally invasive probe and Raman imaging technology according to claim 1, characterized in that: A refrigeration unit is arranged outside the Raman imaging device.

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