Anti-aging composition for activating longevity gene and promoting endogenous glowing and application of anti-aging composition

Abstract

The invention relates to an anti-aging composition for activating longevity genes and promoting endogenous renewal and application thereof, the anti-aging composition comprises ginkgo leaf extract and rice fermentation liquor, and the mass ratio of the ginkgo leaf extract to the rice fermentation liquor is (0.1-4.0): (0.1-10). Compared with the prior art, two efficient anti-aging components including the ginkgo leaf extract and the rice fermentation liquor are combined, the synergistic effect among the components is achieved through scientific and reasonable formula design, and the overall anti-aging effect of the product is improved. Meanwhile, the product disclosed by the invention has the quality characteristic of being easy to control, and the stability and the safety of the product are ensured. According to the scheme, a comprehensive, deep and efficient skin anti-aging effect is achieved through the multi-mechanism synergistic effect, innovative intervention aiming at aging fibroblasts, tight junction protein expression promotion, epidermal renewal and dermal matrix synthesis promotion, anti-inflammatory and anti-oxidation effects, efficient component combination, formula optimization and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and in particular relates to an anti-aging composition capable of activating longevity genes and promoting endogenous rejuvenation, and application thereof. Background Art

[0002] At present, most anti-aging technologies only focus on a specific mechanism or ingredient, such as anti-oxidation, anti-inflammation or a single nutritional supplement, and lack a comprehensive and in-depth understanding of the complex physiological process of skin aging. This one-sidedness leads to the anti-aging effect being often limited to the improvement of surface symptoms, and it is difficult to touch the root cause of aging.

[0003] Chinese patent CN117919133A discloses a baby anti-crack cream, including the following components by mass: 31.64619-78.02302 parts of solvent; 6.1-21 parts of emollient; 6.64898-18.84881 parts of moisturizer; 6.663-22.58 parts of skin conditioner; 1-3 parts of emulsifier; 1.215-2.075 parts of antioxidant; 0.25-0.65 parts of thickener; 0.1-0.2 parts of astringent; the skin conditioner at least includes ginkgo leaf extract, bletilla extract, polygonatum extract, crabapple seed oil, and baobab seed oil. This solution can effectively condition the skin by combining ginkgo leaf extract, bletilla extract, polygonatum extract, crabapple seed oil, and baobab seed oil, so that the skin has better water-locking performance and effectively prevents the skin from cracking and drying.

[0004] Chinese patent CN117582388A discloses an antioxidant composition and its use. The antioxidant composition is composed of olive leaf, persimmon leaf, and ginkgo leaf extracts. When the olive leaf, persimmon leaf, and ginkgo leaf extracts are used in a certain proportion, the antioxidant effect is significantly better than using the above extracts alone.

[0005] Anti-aging is a multi-factor, multi-level biological process, including gene expression, cell metabolism, immune response, etc. However, existing technologies often lack systematic research on the interactions between these factors and the overall regulatory mechanism, making anti-aging strategies appear isolated and of limited effectiveness.

[0006] At present, most of the patents related to Ginkgo biloba extract focus on the extraction and purification technology of active ingredients at the surface level, as well as the exploration of basic functions such as anti-oxidation and anti-inflammatory, striving to efficiently extract key ingredients such as flavonoids and lactones, and verify its effects in single dimensions such as moisturizing, whitening, and anti-wrinkle. However, these research perspectives are generally limited and fail to deeply explore the deep mechanism of Ginkgo biloba extract in the field of anti-aging - especially how it can achieve a more comprehensive and far-reaching anti-aging effect by regulating the immune system and promoting the natural removal of senescent cells. Summary of the invention

[0007] Based on the current situation that the existing skin anti-aging effect is limited, the present invention provides an anti-aging composition for activating longevity genes and promoting endogenous rejuvenation and its application.

[0008] The present application scheme combines multiple physiological mechanisms to achieve a comprehensive anti-aging effect by curbing the immune escape strategy of aging fibroblasts, regulating the expression spectrum of inflammatory factors, promoting epidermal renewal and dermal matrix synthesis, and other comprehensive effects.

[0009] The purpose of the present invention can be achieved by the following technical solutions:

[0010] The invention provides an anti-aging composition for activating longevity genes and promoting endogenous rejuvenation, comprising ginkgo leaf extract and rice fermentation liquid, wherein the mass ratio of the ginkgo leaf extract to the rice fermentation liquid is (0.1-4.0): (0.1-10).

[0011] In one embodiment of the present invention, the mass ratio of the ginkgo leaf extract to the rice fermentation broth is (0.5-2.5):(0.1-5.0).

[0012] In one embodiment of the present invention, the ginkgo leaf extract can be obtained through commercial channels, or can be extracted from ginkgo leaves.

[0013] In one embodiment of the present invention, the rice fermentation liquid is a relatively common additive in the field of cosmetics. It should be noted that the rice fermentation liquid can be purchased commercially or prepared by oneself.

[0014] As a non-limiting example, yeast Saccharomyces veronae can be used to prepare rice fermentation broth through fermentation and enrichment processes, wherein Alpha-KG is enriched, and the mass proportion of Alpha-KG in the rice fermentation broth is 400ppm-1000ppm. The preparation method of rice fermentation broth includes:

[0015] Step a. Add water to the rice powder and heat and stir to form 30% rice milk;

[0016] Step b. adding β-glucanase to the rice milk, the enzyme added in an amount of 0.001%-0.5% by mass of the rice milk, the enzyme activity is 150-200U / g, and enzymolysis is performed to obtain a rice milk hydrolyzate;

[0017] Step c. The yeast strain is inoculated into the rice milk hydrolyzate, and the fermentation process conditions are: the fermentation temperature is 20-40 ° C, the fermentation time is 24-48 hours, the pH of the fermentation process is controlled between 6-6.5, and the rice fermentation liquid is obtained after fermentation;

[0018] Step d. The fermented rice fermentation liquid is concentrated by reverse osmosis technology to efficiently enrich the active substances, and the concentrated rice fermentation liquid is obtained by heating, sterilizing, cooling and filtering, i.e., the rice fermentation liquid containing the active substances.

[0019] The present invention further provides the use of an anti-aging composition for activating longevity genes and promoting endogenous rejuvenation in the preparation of skin care products, wherein the skin care products may be any form of skin care products or cosmetics, including lotions, creams, freeze-dried powders, skin care water, etc. The skin care products have the anti-aging effects of activating longevity genes and promoting endogenous rejuvenation.

[0020] The present invention also provides an anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation, comprising a matrix auxiliary material and functional ingredients, wherein the functional ingredients comprise an anti-aging composition for activating longevity genes and promoting endogenous rejuvenation.

[0021] In some preferred embodiments, based on 100 parts by weight of the total weight of the skin care product, the anti-aging composition for activating longevity genes and promoting endogenous rejuvenation is 1 to 10 parts, preferably 2 to 7.5 parts, and more preferably 3.5 to 7.5 parts.

[0022] In some embodiments of the present invention, the matrix excipient is selected from any one or more of a solvent, a skin conditioner, a thickener, a moisturizer, an emulsifier, a pH adjuster, a fragrance or a preservative.

[0023] In a specific embodiment of the present invention, the present invention provides an anti-aging essence for activating longevity genes and promoting endogenous rejuvenation, the essence comprising phase A excipients, phase B excipients, phase C excipients, phase D functional ingredients and phase E excipients;

[0024] Based on 100 parts by weight of the essence, the phase A auxiliary material includes 0.02 to 8 parts of thickener A, 0.15 to 10.5 parts of skin conditioner A and 1.5 to 15 parts of moisturizer;

[0025] Based on 100 parts by weight of the essence, the phase B auxiliary material includes 0.3 to 5 parts of thickener B, 0.1 to 5 parts of emulsifier and 0.1 to 5 parts of skin conditioner B;

[0026] Based on 100 parts by weight of the essence, the phase C auxiliary material includes 0.1 to 5 parts of a pH adjuster;

[0027] Based on 100 parts by weight of the essence, the amount of the D phase functional ingredient is 1 to 10 parts, preferably 2 to 7.5 parts, and more preferably 3.5 to 7.5 parts;

[0028] Based on 100 parts by weight of the essence, the phase E auxiliary material includes 0.01 to 2 parts of fragrance and 0.15 to 1 part of preservative;

[0029] The phase A auxiliary material also includes water, and the amount of water used is such that the weight parts of the essence reaches 100 parts.

[0030] The thickener A and the thickener B are independently selected from one or more of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide & C13-14 isoalkane & laureth-7, xanthan gum, propylene glycol, behenyl alcohol, carbomer, polyquaternium-37, cetyl alcohol, stearyl alcohol or hydroxyethyl cellulose;

[0031] The skin conditioning agent A and the skin conditioning agent B are each independently selected from one or more of glycerin, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, butylene glycol, squalane, panthenol, lactic acid or tocopherol;

[0032] The moisturizing agent is selected from one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, ethylhexylglycerin, hydrogenated grape seed oil, olive oil or shea butter;

[0033] The emulsifier is selected from one or more of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl trimethyl ammonium methyl sulfate or cocoglyceryl;

[0034] The pH regulator is selected from one or more of arginine or lactic acid;

[0035] The aromatic agent is selected from essences;

[0036] The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin or pentylene glycol.

[0037] In one embodiment of the present invention, the anti-aging essence for activating longevity genes and promoting endogenous rejuvenation, based on 100 parts by weight of the essence, the phase A auxiliary materials include 0.01 to 5 parts of xanthan gum, 0.01 to 5 parts of carbomer, 0.05 to 1 part of sodium hyaluronate, 0.1 to 10 parts of panthenol, 1 to 10 parts of glycerol and 0.5 to 8 parts of butylene glycol; the phase B auxiliary materials include 0.2 to 4 parts of behenyl alcohol, 0. 1-4 parts of hydroxyethyl cellulose, 0.1-5 parts of cetearyl glucoside (and) cetearyl alcohol, 0.1-5 parts of squalane; the C phase auxiliary material includes 0.1-5 parts of arginine; the D phase effective ingredient is 1-10 parts, preferably 2-7.5 parts, more preferably 3.5-7.5 parts; the E phase auxiliary material includes 0.1-1 part of phenoxyethanol, 0.05-1 part of ethylhexylglycerin and 0.01-2 parts of fragrance.

[0038] The present invention also provides a method for preparing the above-mentioned specific anti-aging essence for activating longevity genes and promoting endogenous rejuvenation, comprising the following steps:

[0039] a. Obtaining the aqueous phase: Place water in a container with a stirring device and stir, fully wet and mix the other parts of the A phase excipients, add them to the water, stir until completely dissolved, and heat to 75-80°C;

[0040] b. Obtain the oil phase; mix the B phase excipients and heat to 75-80°C, stirring until completely dissolved;

[0041] c. Emulsification: Add the mixture of step b to the aqueous phase of step a, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes;

[0042] d. Add phase C auxiliary material, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes.

[0043] Stir and cool to 45°C;

[0044] e. Add E phase auxiliary materials, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes;

[0045] f. After the temperature drops to 35-38°C, add phase D, stir at 250-350 rpm and homogenize at 2500-3500 rpm for 3-10 minutes to obtain the desired essence.

[0046] It should be noted that the above steps all require strict control of sterility. The obtained essence is filtered and sterilized before filling, and the bacterial content, toxins, immunogenicity, etc. are tested for quality control.

[0047] Skin aging is a complex process driven by multiple physiological mechanisms, among which the accumulation of senescent fibroblasts and their highly developed immune escape strategies are particularly prominent.

[0048] Fibroblasts are important cellular components of the dermis, responsible for the synthesis of matrix components and maintaining skin elasticity. However, with age, fibroblasts gradually lose their ability to divide and transform into a senescent state.

[0049] These cells significantly interfere with the renewal cycle of the epidermis and the steady-state synthesis of the dermal matrix by secreting senescence-associated secretory phenotype (SASP) mediators, including matrix metalloproteinases (MMPs) and interleukin-6 (IL-6), thereby accelerating the aging process of the skin. At the same time, senescent fibroblasts adopt a strategy of reducing the expression of immune recognition markers such as major histocompatibility complex (MHC) molecules, co-stimulatory molecules and NKG2D ligands (such as MICA, ULBPs), and secreting immunosuppressive cytokines, thus building a strong immune escape barrier and effectively evading the surveillance and clearance of immune effector cells such as natural killer cells (NK cells).

[0050] Ginkgo leaf extract, as an active ingredient derived from nature, has demonstrated powerful intervention capabilities against the above-mentioned aging mechanisms.

[0051] Ginkgo biloba extract effectively curbs the excessive secretion of MMPs, thereby blocking the degradation process of key immune recognition signals on the surface of senescent fibroblasts, making them the target of the immune system again, especially restoring the recognition and clearance ability of NK cells. In addition, Ginkgo biloba extract also significantly downregulated the expression level of immunosuppressive cytokines such as IL-6, further weakened the immune escape ability of senescent fibroblasts, and enhanced the overall effectiveness of the immune system. At the same time, Ginkgo biloba extract can also deeply regulate the expression spectrum of inflammatory factors, effectively reduce the total amount of SASP and its biological activity, thereby optimizing the signal transduction network between cells, reducing unnecessary inflammatory reactions, and providing strong support for the homeostasis of the skin microenvironment. Ginkgo biloba extract not only promotes the healthy renewal of the epidermal stratum corneum, but also significantly enhances the synthetic activity of the dermal matrix, especially promotes the reconstruction and reinforcement of the collagen network, thereby improving the elasticity and firmness of the skin. At the same time, it can also effectively improve the glossiness of the skin color, and enhance the reflectivity and overall beauty of the skin color by regulating the skin microcirculation mechanism.

[0052] Tight junction proteins are key components of the skin barrier. They prevent harmful substances from invading the skin by forming a physical barrier while keeping the moisture and nutrients inside the skin from being lost. Therefore, the expression level of tight junction proteins is directly related to the health and youthfulness of the skin.

[0053] Rice fermentation liquid can significantly promote the expression of tight junction proteins (such as Claudin-1, -4 and Occludin) in epidermal keratinocytes. The increase of these tight junction proteins not only enhances the integrity of the skin barrier, but also improves the skin's water retention capacity and resistance to external stimuli.

[0054] The various growth factors and nutrients in the rice fermented liquid can stimulate the proliferation and differentiation of epidermal keratinocytes and accelerate the repair process of damaged skin. This improvement in self-repair ability not only helps to reduce skin inflammation and damage, but also makes the skin healthier, smoother and more elastic.

[0055] The succinic acid, choline and antioxidant enzymes produced during the fermentation process have significant anti-inflammatory and antioxidant effects. They can neutralize free radicals, reduce oxidative stress and inhibit the occurrence of inflammatory reactions, thereby protecting the skin from external environmental damage and delaying the aging process.

[0056] The present invention combines two highly effective anti-aging ingredients, namely, ginkgo leaf extract and rice fermentation liquid, and achieves a synergistic effect between the ingredients through a scientific and reasonable formula design, thereby improving the overall anti-aging effect of the product. At the same time, the product of the present invention has a quality characteristic that is easy to control, thereby ensuring the stability and safety of the product.

[0057] The present invention uses ginkgo leaf extract to effectively inhibit immune escape-related factors such as MMPs and IL-6 secreted by senescent fibroblasts, restore the recognition and removal ability of NK cells on senescent fibroblasts, and reduce the negative impact of senescent fibroblasts on the skin from the root. In addition, ginkgo leaf extract can stimulate the proliferation and differentiation of epidermal keratinocytes, promote healthy renewal of the epidermis, and significantly enhance the synthetic activity of the dermal matrix, especially promote the reconstruction and reinforcement of the collagen network, and improve the elasticity and firmness of the skin.

[0058] At the same time, rice fermentation liquid significantly promotes the expression of tight junction proteins in epidermal keratinocytes, enhances the integrity and water retention capacity of the skin barrier, and improves the skin's resistance to external stimuli, thereby delaying the skin aging process. The ingredients such as succinic acid, choline and antioxidant enzymes produced during the fermentation process have significant anti-inflammatory and antioxidant effects, which can neutralize free radicals, reduce oxidative stress reactions and inhibit inflammatory reactions, protect the skin from external environmental damage, and further delay the aging process.

[0059] Compared with the prior art, the present invention has the following technical effects:

[0060] (1) This application scheme achieves a comprehensive, in-depth and efficient anti-aging effect on the skin through the synergistic effects of multiple mechanisms, innovative intervention targeting senescent fibroblasts, promotion of tight junction protein expression, promotion of epidermal renewal and dermal matrix synthesis, anti-inflammatory and antioxidant effects, and high-efficiency ingredient combination and formula optimization.

[0061] (2) Describes the multiple physiological mechanisms of skin aging, especially the accumulation of senescent fibroblasts and their immune escape strategies. Emphasizes the role of fibroblasts in skin aging and how they accelerate skin aging by secreting SASP mediators and adopting immune escape strategies.

[0062] (3) In-depth studies were conducted on how Ginkgo biloba extract intervenes in multiple aspects of senescent fibroblasts (such as MMPs secretion, expression of immune recognition markers, and expression of immunosuppressive cytokines). DETAILED DESCRIPTION

[0063] The present invention discloses a composition for activating longevity genes and promoting endogenous rejuvenation. The composition comprises ginkgo leaf extract and rice fermentation broth. The mass ratio of the ginkgo leaf extract to the rice fermentation broth is (0.1-4.0):(0.1-10), preferably (0.5-2.5):(0.1-5.0).

[0064] The present invention also provides an anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation, comprising a matrix excipient and an effective component, wherein the effective component comprises an anti-aging composition for activating longevity genes and promoting endogenous rejuvenation. Based on the total weight of the skin care product being 100 parts by weight, the anti-aging composition for activating longevity genes and promoting endogenous rejuvenation is 1 to 10 parts, preferably 2 to 7.5 parts, and more preferably 3.5 to 7.5 parts.

[0065] The following are some specific embodiments to illustrate the present invention. These embodiments are only used to further illustrate the present invention, and are not used to limit the present invention, and do not represent a limitation on the protection scope of the present invention. Some non-essential modifications and adjustments made by others based on the present invention still fall within the protection scope of the present invention.

[0066] The present invention takes essence as an example to set various groups of embodiments and comparative examples, but is not limited to the use of essence. The composition can also be applied to various skin care products such as lotion, cream, freeze-dried powder, skin care water, etc. The essence is the above-mentioned key combination and skin care agent, thickener, skin conditioner and pH regulator. Preferably, the raw materials for the preparation of the essence that enhances the extracellular matrix (ECM) and has barrier repair effect also include any one or a combination of at least two of preservatives and flavors.

[0067] Unless otherwise specified, the percentages (%) below are all by mass. All raw materials of the present invention are commercially available products.

[0068] The present invention is described in detail below with reference to specific embodiments.

[0069] Example 1

[0070] An anti-aging serum that activates longevity genes and promotes endogenous rejuvenation, including a key component with anti-aging effects.

[0071] The raw material component distribution ratio of this embodiment is shown in Table 1.

[0072] Table 1: Ratio of components in Example 1

[0073]

[0074]

[0075] The preparation method of the essence of this embodiment comprises the following steps:

[0076] a. Obtaining the aqueous phase: Place water in a container with a stirring device and stir, fully wet and mix the other parts of the A phase excipients, add them to the water, stir until completely dissolved, and heat to 75-80°C;

[0077] b. Obtain the oil phase; mix the B phase excipients and heat to 75-80°C, stirring until completely dissolved;

[0078] c. Emulsification: Add the mixture of step b to the aqueous phase of step a, stir at 300 rpm for 4 minutes and homogenize at 3000 rpm for 5 minutes;

[0079] d. Add phase C auxiliary material, stir at 300 rpm for 4 minutes and homogenize at 3000 rpm for 5 minutes, and cool to 45°C with stirring;

[0080] e. Add phase E auxiliary material, stir at 300 rpm for 4 minutes and homogenize at 3000 rpm for 5 minutes;

[0081] f. After the temperature drops to 35-38°C, add phase D, stir at 300 rpm and homogenize at 3000 rpm for 5 minutes to obtain the desired essence.

[0082] Examples 2, 3 and Comparative Examples 1, 2, 3

[0083] The raw material composition ratios of Examples 2 and 3 and Comparative Examples 1, 2 and 3 are shown in Table 2. The difference between Example 2 and Example 1 is only the increase of each effective ingredient, and the difference between Example 3 and Example 1 is only the reduction of each effective ingredient.

[0084] The difference between Comparative Examples 1-2 and Example 1 is only the lack of one key composition component. The difference between Comparative Example 3 and Example 1 is only the lack of all key composition components.

[0085] The specific ratios are shown in Table 2. The preparation method is the same as that in Example 1, except that in step f, the substances in phase D are adaptively added or not added (Comparative Example 3).

[0086] Table 2 Ratio of raw material components of Examples 1-3 and Comparative Examples 1-3

[0087]

[0088] Efficacy evaluation test

[0089] Table 3 Cell experiment raw material composition ratio

[0090] INCI name of raw material Experimental Example 1 Experimental Example 2 Experimental Example 3 Blank example 1 Blank example 2 Blank example 3 water to 100 to 100 to 100 to 100 to 100 to 100 Ginkgo Biloba Extract 1.5 2.5 1 0 3.5 0 Rice fermentation liquid 2 5 1 3.5 0 0

[0091] 1. Test on the effect of regulating immune escape senescent human fibroblasts

[0092] 1) Senescent human fibroblasts were cultured at 10 3 -10 4 The cells were seeded at a density of 100 cells / well in a 24-well plate, 100 μL of DMEM medium was added, and the cells were cultured at 37°C in a CO2 incubator for 24 hours.

[0093] 2) Add the samples of Experimental Examples 1-3 and Blank Examples 1-3 to the plate and incubate in an incubator for 48 hours.

[0094] 3) Collect the cell culture supernatant, use an ELISA kit, add the supernatant of Experimental Examples 1-3 and Blank Examples 1-3 to the corresponding reaction wells in the ELISA plate, and perform subsequent ELISA analysis.

[0095] The ELISA kit includes: MMP-1, MMP-3, IL-6 and MICA specific antibodies and standards.

[0096] 4) The absorbance at 450 nm was measured using an ELISA reader, and the concentrations of MMP-1, MMP-3, IL-6, and MICA in the sample were calculated based on the standard curve.

[0097] 5) With blank example 3 as a reference, the inhibition rates of experimental examples 1-3 and blank examples 1-3 on the synthesis of MMP-1, MMP-3 and IL-6 were calculated, synthesis inhibition rate (%) = (absorbance of test group - absorbance of blank example 3) / absorbance of blank example 3 * 100%; cleavage inhibition rate (%): (absorbance of blank example 3 - absorbance of test group) / absorbance of blank example 3 * 100%. The test results are shown in Tables 4-7 below.

[0098] Table 4 MMP-1 synthesis inhibition rate

[0099]

[0100]

[0101] Table 5 MMP-3 synthesis inhibition rate

[0102] sample MMP-3 synthesis inhibition rate % Experimental Example 1 20.31 Experimental Example 2 27.93 Experimental Example 3 13.88 Blank example 1 5.63 Blank example 2 22.42 Blank example 3 0.00

[0103] Table 6 IL-6 inhibition rate

[0104] sample IL-6 synthesis inhibition rate % Experimental Example 1 75.69 Experimental Example 2 87.35 Experimental Example 3 64.16 Blank example 1 11.38 Blank example 2 76.24 Blank example 3 0.00

[0105] Table 7 MICA cleavage inhibition rate

[0106]

[0107]

[0108] As shown in Tables 4-7, after cell culture using the essences of Experimental Examples 1-3 and Blank Examples 1-2, the synthesis content of MMP-1, MMP-3, and IL-6 in senescent human fibroblasts was significantly reduced. And the results of Experimental Examples 1-3 and Blank Example 2 were significantly better than those of Blank Example 1, indicating that Ginkgo biloba extract can curb the excessive secretion of MMPs and downregulate the expression level of IL-6 immunosuppressive cytokine, weaken the immune escape ability of senescent fibroblasts, and enhance the overall effectiveness of the immune system.

[0109] 2. Promotion of epidermal tight junction proteins (Claudin-1 and Claudin-4)

[0110] Test method:

[0111] 1) Normal human epidermal keratinocytes (NHEK) were cultured at 75 cm 2 The cells were collected in the logarithmic phase and cultured at a cell density of 1×10 5 Cells / 500 μL were seeded in 8-well culture plates and cultured overnight.

[0112] 2) After the culture, 500 μL of fresh culture medium was taken and added to Experimental Examples 1-3 and Blank Examples 1-3, respectively, and then the cells were cultured for 24 hours and then fixed on each well.

[0113] 3) Claudin-1 was immunofluorescently stained with rabbit anti-Claudin-1 polyclonal antibody, and the cell nuclei were fluorescently stained with DAPI. The promotion of Claudin-1 production was observed using a fluorescence microscope. Claudin-4 was immunofluorescently stained with mouse anti-Claudin-4 monoclonal antibody, and the antibody and cell nuclei were fluorescently stained with DAPI. The promotion of Claudin-4 was observed using a fluorescence microscope.

[0114] 4) Using Blank Example 3 as a reference, calculate the Claudin-1 promotion rate, Claudin-1 promotion rate (%) = (Claudin-1 value of the test group - Claudin-1 value of Blank Example 3) / Claudin-1 value of Blank Example 3 * 100%. The test results are shown in Table 8 below.

[0115] 5) Using Blank Example 3 as a reference, calculate the Claudin-4 promotion rate, Claudin-4 promotion rate (%) = (Claudin-4 value of the test group - Claudin-4 value of Blank Example 3) / Claudin-4 value of Blank Example 3 * 100%. The test results are shown in Table 9 below.

[0116] Table 8 Claudin-1 promotion rate %

[0117] sample Claudin-1 promotion rate % Experimental Example 1 20.62 Experimental Example 2 24.58 Experimental Example 3 16.59 Blank example 1 9.83 Blank example 2 7.35 Blank example 3 0.00

[0118] Table 9 Claudin-4 promotion rate %

[0119] sample Claudin-4 promotion rate % Experimental Example 1 14.74 Experimental Example 2 17.81 Experimental Example 3 11.53 Blank example 1 7.21 Blank example 2 6.35 Blank example 3 0.00

[0120] As shown in Tables 8 and 9, after cell culture using the essences of Experimental Examples 1-3 and Blank Examples 1-2, the Claudin-1 promotion rate and Claudin-4 promotion rate were significantly improved. However, compared with Blank Examples 1-2, the improvement effect of Experimental Examples 1-3 is more significant. From the comparison of the results, it can be seen that when Ginkgo Biloba Extract and Rice Fermentation Broth are used at the same time, the Claudin-1 promotion rate and Claudin-4 promotion rate are more significantly improved, compared with not using an anti-aging composition that activates longevity genes and promotes endogenous rejuvenation, or only using one of Ginkgo Biloba Extract and Rice Fermentation Broth. This shows that Ginkgo Biloba Extract and Rice Fermentation Broth have a synergistic effect, which can enhance the formation of tight junction protein barriers, enhance the integrity of the skin barrier, and have an anti-aging effect.

[0121] Characterization data and effect data of the products of the embodiments and comparative examples

[0122] 1. Security Testing

[0123] 1) Test sample: the essence of Examples 1-3 and Comparative Examples 1-3 described in Table 2.

[0124] 2) Number of participants: 70, half male and half female, aged between 18 and 45 years old, all meeting the criteria for subjects.

[0125] 3) Test method: 70 subjects who met the test criteria were randomly divided into 7 groups, corresponding to the essences obtained in Examples 1-3 and Comparative Examples 1-3 described in Table 2, and blank group 1 was not applied with any sample, with 10 subjects in each group; safety was tested by a third-party testing agency. The selected area should not exceed 50mm 2 , a suitable patch with a depth of about 1mm, add about 0.02-0.025ml (g) of the test substance into the patch tester by the closed patch test method. The patch tester is attached to the curved side of the subject's forearm, and the test substance is removed after 24 hours. The skin reaction is observed at 0.5h, 24h, and 48h after removal: the results are recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Regulations 2015".

[0126] Table 10 Patch test results

[0127]

[0128]

[0129] Note: Skin reaction grading standards:

[0130] Grade 0: negative reaction;

[0131] Grade 1: Suspicious reaction; only slight erythema;

[0132] Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0133] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes: the reaction may extend beyond the tested area;

[0134] Grade 4: Extremely strong reaction (confluent herpes reaction): Obvious erythema, severe infiltration, edema, confluent herpes, and the reaction extends beyond the tested area.

[0135] 3) Test results: The results in Table 10 show no irritation and are considered safe.

[0136] 2. Stability test

[0137] 1) Test samples: Examples 1-3 described in Table 2.

[0138] 2) Test method: Internal control standard, observe the appearance color, viscosity and pH for three months.

[0139] Table 11 Stability test results

[0140] time 45℃ 40℃ 37℃ 25℃ 5℃ -15℃ CYCLE illumination 1W √ √ √ √ √ √ √ √ 2W √ √ √ √ √ √ √ √ 3W √ √ √ √ √ √ √ √ 4W √ √ √ √ √ √ √ √ 2M √ √ √ √ √ √ √ √ 3M √ √ √ √ √ √ √ √

[0141] Among them, "CYCLE" refers to the cycle, and the cycle setting is: heating from -15℃ to 45℃, cooling from 45℃ to -15℃ for five times.

[0142] Test results: The results in Table 11 show that the essences in Examples 1-3 are stable in properties within 3 months.

[0143] 3. Cuticle moisture content test

[0144] Corneometer CM825 is used to measure the moisture content of the stratum corneum. The higher the measured value, the higher the moisture content of the stratum corneum of the skin.

[0145] 1) Test sample: the essence of Examples 1-3 and Comparative Examples 1-3 described in Table 2.

[0146] 2) Test subjects: 60 healthy women (21-45 years old, average age 36.3 years old) were randomly divided into 6 groups, each with 10 people, regardless of gender, and each group used the same formula product. Written informed consent was signed. Before enrollment, the subjects were asked a series of questions about their medical history and health status according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0147] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0148] 3) Test method: Use the sample twice a day, morning and evening, for 8 weeks. Apply an appropriate amount of the test sample on one cheek, and do not use the test sample on the other cheek. Apply sunscreen products to the entire face every morning.

[0149] The test results were collected at the following four time periods: before using the sample (D0), 14 days after using the sample (D14), 28 days after using the sample (D28), and 56 days after using the sample (D56).

[0150] The test results can be calculated using the following formula:

[0151] Change rate = (average analysis value of 10 people in each group after using the product - average analysis value of 10 people in each group before using the product) ÷ average analysis value of 10 people in each group before using the product × 100%

[0152] Table 12 Change rate of moisture content in stratum corneum

[0153] sample After 14 days of use % After 28 days of use % After 56 days of use % Example 1 17.26 20.83 33.75 Example 2 18.63 23.56 35.23 Example 3 13.75 16.75 26.84 Comparative Example 1 8.38 11.34 18.46 Comparative Example 2 9.94 12.81 21.93 Comparative Example 3 6.72 8.96 15.29

[0154] As shown in Table 12, after using the essence, as the use time increases, the change rate of the water content of the stratum corneum of the skin of Examples 1-3 and Comparative Examples 1-3 increases, indicating that the water content of the stratum corneum of the skin has improved. However, compared with Comparative Examples 1-3, when used for the same time, the change rate of the water content of the stratum corneum of Examples 1-3 is greater, indicating that the improvement effect of the water content of the stratum corneum of the skin after using the essence of Examples 1-3 is more significant.

[0155] From the comparison of the results of Examples 1-3 and Comparative Examples 1-3, it can be seen that when the ginkgo leaf extract and the rice fermentation liquid are used together, the improvement effect of the water content of the stratum corneum of the skin is better than that of not using an anti-aging composition that activates longevity genes and promotes endogenous rejuvenation, or using only one of the ginkgo leaf extract and the rice fermentation liquid. This shows that the ginkgo leaf extract and the rice fermentation liquid have a synergistic effect and can effectively improve the water content of the stratum corneum of the skin.

[0156] 4. Assessment of crow's feet

[0157] The higher the crow's feet score, the more severe the wrinkles.

[0158] 1) Test sample: the essence of Examples 1-3 and Comparative Examples 1-3 described in Table 2.

[0159] 2) Test subjects: 60 healthy women (21-45 years old, average age 36.3 years old) were randomly divided into 6 groups, each with 10 people, regardless of gender, and each group used the same formula product. Written informed consent was signed. Before enrollment, the subjects were asked a series of questions about their medical history and health status according to the inclusion and exclusion criteria. At the same time, the skin of the test site was evaluated for compliance and skin color test screening, and the results were recorded;

[0160] Environmental conditions: During the test, visual assessment and instrument testing are carried out in an environment with a temperature of 21±1℃ and a relative humidity of 50±10% RH. Visual assessment is carried out under constant lighting conditions (fluorescent lamps or LED lighting with a color temperature of 5500-6500K). The subjects need to adapt to this environmental condition for at least 30 minutes before evaluation and testing can be carried out.

[0161] 3) Test method: Use the sample twice a day, morning and evening, for 8 weeks. Apply an appropriate amount of the test sample around one eye, and do not use the test sample around the other eye. Apply sunscreen products to the entire face every morning.

[0162] The test results were collected at the following four time periods: before using the sample (D0), 14 days after using the sample (D14), 28 days after using the sample (D28), and 56 days after using the sample (D56).

[0163] The test results can be calculated using the following formula:

[0164] Change rate = (analysis value after using the product - analysis value before using the product) ÷ analysis value before using the product × 100%

[0165] Table 13 Crow's feet test value change rate

[0166] sample After 14 days of use % After 28 days of use % After 56 days of use % Example 1 -6.87 -12.63 -24.24 Example 2 -7.59 -14.77 -26.53 Example 3 -5.36 -10.14 -20.08 Comparative Example 1 -3.14 -7.28 -14.74 Comparative Example 2 -4.23 -7.65 -15.92 Comparative Example 3 -0.79 -2.82 -7.29

[0167] From the results in Table 13, it can be seen that after using the essence, as the use time increases, the change rate of crow's feet wrinkles in Examples 1-3 and Comparative Examples 1-3 decreases, indicating that crow's feet wrinkles are improved. However, compared with Comparative Examples 1-3, when used for the same time, the change rate of crow's feet wrinkles in Examples 1-3 decreases more, indicating that the improvement effect of crow's feet wrinkles after using the essence of Examples 1-3 is more significant.

[0168] From the comparison of the results of Examples 1-3 and Comparative Examples 1-3, it can be seen that when the ginkgo leaf extract and the rice fermentation liquid are used together, the improvement effect of crow's feet wrinkles is better than that of not using an anti-aging composition that activates longevity genes and promotes endogenous rejuvenation, or using only one of the ginkgo leaf extract and the rice fermentation liquid. This shows that the ginkgo leaf extract and the rice fermentation liquid have a synergistic effect and can effectively slow down skin aging.

[0169] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. An anti-aging composition for activating longevity genes and promoting endogenous rejuvenation, characterized in that: The invention comprises ginkgo leaf extract and rice fermentation liquor, wherein the mass ratio of the ginkgo leaf extract to the rice fermentation liquor is (0.1-4.0):(0.1-10).

2. The anti-aging composition for activating longevity genes and promoting endogenous rejuvenation according to claim 1, characterized in that: The mass ratio of the ginkgo leaf extract to the rice fermentation liquid is (0.5-2.5):(0.1-5.0).

3. Use of the anti-aging composition for activating longevity genes and promoting endogenous rejuvenation according to any one of claims 1 to 2 in the preparation of skin care products.

4. An anti-aging skin care product that activates longevity genes and promotes endogenous rejuvenation, characterized in that: The invention comprises matrix excipients and functional ingredients, wherein the functional ingredients comprise the anti-aging composition for activating longevity genes and promoting endogenous rejuvenation as described in any one of claims 1 to 2.

5. The anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation according to claim 4, characterized in that: Based on 100 parts by weight of the total weight of the skin care product, the anti-aging composition for activating longevity genes and promoting endogenous rejuvenation is 1 to 10 parts, preferably 2 to 7.5 parts, and more preferably 3.5 to 7.5 parts.

6. The anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation according to claim 4, characterized in that: The matrix auxiliary material is selected from any one or more of solvents, skin conditioners, thickeners, moisturizers, emulsifiers, pH regulators, fragrances or preservatives.

7. The anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation according to claim 4, characterized in that: The skin care product is an essence, which includes phase A auxiliary materials, phase B auxiliary materials, phase C auxiliary materials, phase D functional ingredients and phase E auxiliary materials; Based on 100 parts by weight of the essence, the phase A auxiliary material includes 0.02 to 8 parts of thickener A, 0.15 to 10.5 parts of skin conditioner A and 1.5 to 15 parts of moisturizer; Based on 100 parts by weight of the essence, the phase B auxiliary material includes 0.3 to 5 parts of thickener B, 0.1 to 5 parts of emulsifier and 0.1 to 5 parts of skin conditioner B; Based on 100 parts by weight of the essence, the phase C auxiliary material includes 0.1 to 5 parts of a pH adjuster; Based on 100 parts by weight of the essence, the amount of the D phase functional ingredient is 1 to 10 parts, preferably 2 to 7.5 parts, and more preferably 3.5 to 7.5 parts; Based on 100 parts by weight of the essence, the phase E auxiliary material includes 0.01 to 2 parts of fragrance and 0.15 to 1 part of preservative; The phase A auxiliary material also includes water, and the amount of water used is such that the weight parts of the essence reaches 100 parts.

8. The anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation according to claim 7, characterized in that: The thickener A and the thickener B are independently selected from one or more of acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, polyacrylamide & C13-14 isoalkane & laureth-7, xanthan gum, propylene glycol, behenyl alcohol, carbomer, polyquaternium-37, cetyl alcohol, stearyl alcohol or hydroxyethyl cellulose; The skin conditioning agent A and the skin conditioning agent B are each independently selected from one or more of glycerin, olive oil, sodium hyaluronate, lauryl alcohol, avocado butter, allantoin, butylene glycol, squalane, panthenol, lactic acid or tocopherol; The moisturizing agent is selected from one or more of hyaluronic acid, allantoin, glycerin, ceramide, sodium lactate, butylene glycol, ethylhexylglycerin, hydrogenated grape seed oil, olive oil or shea butter; The emulsifier is selected from one or more of glyceryl stearate citrate, cetearyl glucoside (and) cetearyl alcohol, behenyl trimethyl ammonium methyl sulfate or cocoglyceryl; The pH regulator is selected from one or more of arginine or lactic acid; The aromatic agent is selected from essences; The preservative is selected from one or more of phenoxyethanol, ethylhexylglycerin or pentylene glycol.

9. The anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation according to claim 8, characterized in that: Based on 100 parts by weight of the essence, the phase A auxiliary materials include 0.01-5 parts of xanthan gum, 0.01-5 parts of carbomer, 0.05-1 parts of sodium hyaluronate, 0.1-10 parts of panthenol, 1-10 parts of glycerin and 0.5-8 parts of butylene glycol; the phase B auxiliary materials include 0.2-4 parts of behenyl alcohol, 0.1-4 parts of hydroxyethyl cellulose, 0.1-5 parts of cetearyl glucoside (and) cetearyl alcohol, and 0.1-5 parts of squalane; the phase C auxiliary materials include 0.1-5 parts of arginine; the phase D effective ingredients are 1 to 10 parts; the phase E auxiliary materials include 0.1-1 parts of phenoxyethanol, 0.05-1 parts of ethylhexylglycerin and 0.01-2 parts of fragrance.

10. The method for preparing an anti-aging skin care product for activating longevity genes and promoting endogenous rejuvenation according to claim 8, characterized in that: The following steps are involved: a. Obtaining the aqueous phase: Place water in a container with a stirring device and stir, fully wet and mix the other parts of the phase A excipients, add them to the water, stir until completely dissolved, and heat to 75-80°C; b. Obtain the oil phase; mix the B phase excipients and heat to 75-80°C, stirring until completely dissolved; c. Emulsification: Add the mixture of step b to the aqueous phase of step a, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes; d. Add phase C auxiliary material, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes, and cool to 45°C with stirring; e. Add E phase auxiliary materials, stir at 250-350 rpm for 3-5 minutes and homogenize at 2500-3500 rpm for 3-10 minutes; f. After the temperature drops to 35-38°C, add phase D, stir at 25-35 rpm and homogenize at 2500-3500 rpm for 3-10 minutes to obtain the desired essence.

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